Rw2G052180

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012020
Physical Location & Seq
Forward (+)
79986667 .. 79991143
4477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G052180.1

Sequence Viewer

Length: 1308 bp
ATGGGGAATTTGTTCGACAAGGAGCCGCCGCCGCCTATTGTGCTCGTCCCGCCGCTCTTCGATTATCCGCCTCTCGCCGCTCGCACCAGGATGTTGGAGTCGTCATATGACTTGTTGTTTGGGAAGCTTGCTTTGAAAGGTCTGTTTCAGGACTATTTTGAACAAGCAAGGCATTTCAGCACAATGATCATGCTAAAGCCGATTGATGACCCTCATGTGGATTTAGTTGCAACGCTCTCTGGTCCACTTGATCACAAACCTGAGGAGACCATTGTTGGGAATGCATTTTTCCGCTGGCAAAGTGATGTTCATGATCCTCACACATTCGTGGACCTCTTCGTATCAAACTCAGATCCGATTTTGCAGACGAGGGCATGTGCTTACTACCCAAAATTTGGATTTGGAGCATTTGGTGTCTTCCCGGTGCTGCTAAAACAAAGAATAACATCTGAGGACTTTGGTGTTATGGGATTGAGATACGGCTCAGCAAACTTATCAATTGGAGCCACAGTCATGCCTTTCTCTGTGAAAGATGATTTGCCAAGAAAGGCGTGGCTGGTGACCAAAATGGGAAGTCTAACTGCAGGTGTGCAGTATGAGCCAGAATTTGGAAGCAAAGATGGTCAAAAATACAAAGACTTAAAGAATTGGAGTGGTGCGATTGGGTATGGAGTGGGGTCAAGCAGCCCTTTGAGTCCATCGTTCAATTTTGGTCTCGAGCTTTCTAGAAGTAATCAGTTCACTGCCTCGTTCTATCAACATGTGGTGGTCCAAAGACGGGTGAAAAACCCCCTCGAAGAAAATCAAGTTGTTGGGATTACGAATTATATCGATTTTGGCTTTGAATTACAATCAAGGTTTGATGATACCAAAACATCAGACGATGAATCAAACTCAAACAAAGTGCCAGATCCTAAGAATATACCAGATTCCACCTTTCAAATAGCTGCATCTTGGCAAGCCAACAAGAACTTTTTGCTGAAGGGAAAGGTGGGCCCTCTCAGCTCATCGCTAGCATTGGCATTTAAATCCTGGTGGAAACCTTCTTTCACATTCAGCATTTCAGCTGTCAGAGATCACATATTTGGAAATACAGCTTACGGTTTTGGCATTCGTGTTGAGAATATCAGACAAGCCAGCTACCAAAGAGCCGATCCAAATTTTGTAATGTTGACACCAAGTAAGGAGCACCTGGCAGAAGGCATTGTTTGGCAAGCTGGGAAACGGCCGATGCTAGAGTCAAATATAAATGCTGGAAATTTTGACGGCGTACCAATGGAATTACAACCCTTGCGAAAAATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

435

Amino Acids

48.62

Weight (kDa)

6.72

Isoelectric Point (pI)

39.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27930 AT3G27930 AT3G27930
fragaria_vesca FvH4_6g50760
malus_domestica MD09G1032600.v1.1 MD17G1034200.v1.1
prunus_persica Prupe.3G285400_v2.0.a1
pyrus_communis pycom17g03160
rosa_chinensis RchiOBHm_Chr2g0171701
rosa_laevigata RLG00000022030
rosa_multiflora Rmu_ssc0000252.1_g000012
rosa_roxburghii Rroxscaffold_2G00080510
rosa_rugosa Rorug02G0555400
rosa_samantha Rh2BG641000 Rh2BG643500 Rh2CG609200 Rh2DG651900 Rh2DG657200
rosa_wichuraiana Rw2G052180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 575
Acc36I ACCTGC 1 cut(s) 575
AccB7I CCANNNNNTGG 2 cut(s) 395, 608
AccBSI CCGCTC 2 cut(s) 55, 80
AciI CCGC 8 cut(s) 26, 29, 32, 50, 53, 68, 78, 292
AclWI GGATC 4 cut(s) 308, 347, 905, 1148
AcoI YGGCCR 1 cut(s) 1226
AcsI RAATTY 6 cut(s) 7, 392, 605, 1159, 1258, 1299
AcuI CTGAAG 1 cut(s) 1001
AfaI GTAC 1 cut(s) 1272
AfiI CCNNNNNNNGG 5 cut(s) 217, 276, 395, 608, 778
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 5 cut(s) 136, 161, 706, 845, 941
AjnI CCWGG 3 cut(s) 86, 1031, 1191
AleI CACNNNNGTG 1 cut(s) 326
AluBI AGCT 8 cut(s) 127, 721, 947, 1005, 1067, 1097, 1140, 1217
AluI AGCT 8 cut(s) 127, 721, 947, 1005, 1067, 1097, 1140, 1217
Alw21I GWGCWC 2 cut(s) 45, 1191
Alw26I GTCTC 2 cut(s) 260, 719
AlwI GGATC 4 cut(s) 308, 347, 905, 1148
Ama87I CYCGRG 1 cut(s) 716
AoxI GGCC 2 cut(s) 994, 1226
ApaI GGGCCC 1 cut(s) 998
ApeKI GCWGC 3 cut(s) 427, 684, 947
ApoI RAATTY 6 cut(s) 7, 392, 605, 1159, 1258, 1299
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
Asp700I GAANNNNTTC 1 cut(s) 11
AspS9I GGNCC 5 cut(s) 242, 331, 769, 994, 995
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 2 cut(s) 571, 793
AsuNHI GCTAGC 1 cut(s) 1012
AvaI CYCGRG 1 cut(s) 716
AvaII GGWCC 3 cut(s) 242, 331, 769
AxyI CCTNAGG 1 cut(s) 261
BaeGI GKGCMC 1 cut(s) 998
BanII GRGCYC 1 cut(s) 998
BbsI GAAGAC 1 cut(s) 409
Bbv12I GWGCWC 2 cut(s) 45, 1191
BbvI GCAGC 3 cut(s) 414, 696, 934
BccI CCATC 2 cut(s) 614, 706
BceAI ACGGC 3 cut(s) 496, 1241, 1282
BciT130I CCWGG 3 cut(s) 88, 1033, 1193
BclI TGATCA 2 cut(s) 186, 250
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 2 cut(s) 260, 719
BfaI CTAG 3 cut(s) 726, 1013, 1235
BfmI CTRYAG 1 cut(s) 582
BfuAI ACCTGC 1 cut(s) 575
BisI GCNGC 8 cut(s) 26, 29, 32, 53, 78, 428, 685, 948
BlpI GCTNAGC 1 cut(s) 484
BlsI GCNGC 8 cut(s) 27, 30, 33, 54, 79, 429, 686, 949
Bme1390I CCNGG 4 cut(s) 88, 422, 1033, 1193
Bme18I GGWCC 3 cut(s) 242, 331, 769
BmeT110I CYCGRG 1 cut(s) 716
BmgT120I GGNCC 5 cut(s) 242, 331, 769, 994, 995
BmiI GGNNCC 3 cut(s) 24, 505, 996
BmrFI CCNGG 4 cut(s) 88, 422, 1033, 1193
BmsI GCATC 2 cut(s) 959, 1221
BmtI GCTAGC 1 cut(s) 1016
BpiI GAAGAC 1 cut(s) 409
Bpu1102I GCTNAGC 1 cut(s) 484
BpuMI CCSGG 1 cut(s) 422
Bsa29I ATCGAT 1 cut(s) 831
BsaI GGTCTC 2 cut(s) 260, 719
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 5 cut(s) 217, 276, 395, 608, 778
Bse21I CCTNAGG 1 cut(s) 261
BseBI CCWGG 3 cut(s) 88, 1033, 1193
BseCI ATCGAT 1 cut(s) 831
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 5 cut(s) 217, 276, 395, 608, 778
BseMII CTCAG 5 cut(s) 252, 363, 441, 498, 1015
BseRI GAGGAG 1 cut(s) 278
BseSI GKGCMC 1 cut(s) 998
BseX3I CGGCCG 1 cut(s) 1226
BseXI GCAGC 3 cut(s) 414, 696, 934
BseYI CCCAGC 1 cut(s) 1217
BsgI GTGCAG 1 cut(s) 611
Bsh1285I CGRYCG 1 cut(s) 1229
BshFI GGCC 2 cut(s) 996, 1228
BshVI ATCGAT 1 cut(s) 831
BsiEI CGRYCG 1 cut(s) 1229
BsiHKAI GWGCWC 2 cut(s) 45, 1191
BsiHKCI CYCGRG 1 cut(s) 716
BsiSI CCGG 1 cut(s) 422
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 5 cut(s) 217, 276, 395, 608, 778
BsmAI GTCTC 2 cut(s) 260, 719
BsmFI GGGAC 1 cut(s) 32
BsmI GAATGC 2 cut(s) 286, 1110
BsnI GGCC 2 cut(s) 996, 1228
Bso31I GGTCTC 2 cut(s) 260, 719
BsoBI CYCGRG 1 cut(s) 716
Bsp120I GGGCCC 1 cut(s) 994
Bsp1286I GDGCHC 3 cut(s) 45, 998, 1191
Bsp143I GATC 7 cut(s) 186, 250, 313, 352, 910, 1075, 1153
Bsp1720I GCTNAGC 1 cut(s) 484
BspACI CCGC 8 cut(s) 26, 29, 32, 50, 53, 68, 78, 292
BspANI GGCC 2 cut(s) 996, 1228
BspCNI CTCAG 5 cut(s) 253, 362, 442, 497, 1014
BspDI ATCGAT 1 cut(s) 831
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 3 cut(s) 24, 505, 996
BspMAI CTGCAG 1 cut(s) 586
BspMI ACCTGC 1 cut(s) 575
BspOI GCTAGC 1 cut(s) 1016
BspPI GGATC 4 cut(s) 308, 347, 905, 1148
BspQI GCTCTTC 1 cut(s) 62
BspTNI GGTCTC 2 cut(s) 260, 719
BsrBI CCGCTC 2 cut(s) 55, 80
BssMI GATC 7 cut(s) 186, 250, 313, 352, 910, 1075, 1153
Bst2UI CCWGG 3 cut(s) 88, 1033, 1193
Bst4CI ACNGT 2 cut(s) 511, 1103
Bst6I CTCTTC 2 cut(s) 62, 341
BstC8I GCNNGC 7 cut(s) 82, 129, 296, 960, 1014, 1138, 1215
BstDEI CTNAG 6 cut(s) 261, 349, 450, 484, 915, 1001
BstEII GGTNACC 1 cut(s) 559
BstF5I GGATG 1 cut(s) 96
BstKTI GATC 7 cut(s) 189, 253, 316, 355, 913, 1078, 1156
BstMAI GTCTC 2 cut(s) 260, 719
BstMBI GATC 7 cut(s) 186, 250, 313, 352, 910, 1075, 1153
BstMCI CGRYCG 1 cut(s) 1229
BstMWI GCNNNNNNNGC 5 cut(s) 31, 40, 49, 598, 1002
BstNI CCWGG 3 cut(s) 88, 1033, 1193
BstNSI RCATGY 2 cut(s) 378, 764
BstPI GGTNACC 1 cut(s) 559
BstSCI CCNGG 4 cut(s) 86, 420, 1031, 1191
BstSFI CTRYAG 1 cut(s) 582
BstSLI GKGCMC 1 cut(s) 998
BstV1I GCAGC 3 cut(s) 414, 696, 934
BstV2I GAAGAC 1 cut(s) 409
BstX2I RGATCY 2 cut(s) 352, 910
BstXI CCANNNNNNTGG 1 cut(s) 94
BstYI RGATCY 2 cut(s) 352, 910
BstZI CGGCCG 1 cut(s) 1226
Bsu15I ATCGAT 1 cut(s) 831
Bsu36I CCTNAGG 1 cut(s) 261
BsuRI GGCC 2 cut(s) 996, 1228
BsuTUI ATCGAT 1 cut(s) 831
BtgZI GCGATG 1 cut(s) 993
BtsCI GGATG 1 cut(s) 96
BtsI GCAGTG 1 cut(s) 741
BtsIMutI CAGTG 1 cut(s) 741
BveI ACCTGC 1 cut(s) 575
Cac8I GCNNGC 7 cut(s) 82, 129, 296, 960, 1014, 1138, 1215
CciI TCATGA 1 cut(s) 310
Cfr13I GGNCC 5 cut(s) 242, 331, 769, 994, 995
ClaI ATCGAT 1 cut(s) 831
Csp6I GTAC 1 cut(s) 1271
CviAII CATG 6 cut(s) 190, 215, 311, 375, 514, 761
CviQI GTAC 1 cut(s) 1271
DdeI CTNAG 6 cut(s) 261, 349, 450, 484, 915, 1001
DpnI GATC 7 cut(s) 188, 252, 315, 354, 912, 1077, 1155
DpnII GATC 7 cut(s) 186, 250, 313, 352, 910, 1075, 1153
DraI TTTAAA 1 cut(s) 1027
EaeI YGGCCR 1 cut(s) 1226
EagI CGGCCG 1 cut(s) 1226
Eam1104I CTCTTC 2 cut(s) 62, 341
EarI CTCTTC 2 cut(s) 62, 341
EciI GGCGGA 1 cut(s) 57
EclXI CGGCCG 1 cut(s) 1226
Eco24I GRGCYC 1 cut(s) 998
Eco31I GGTCTC 2 cut(s) 260, 719
Eco47I GGWCC 3 cut(s) 242, 331, 769
Eco52I CGGCCG 1 cut(s) 1226
Eco57I CTGAAG 1 cut(s) 1001
Eco81I CCTNAGG 1 cut(s) 261
Eco88I CYCGRG 1 cut(s) 716
Eco91I GGTNACC 1 cut(s) 559
EcoO109I RGGNCCY 1 cut(s) 995
EcoO65I GGTNACC 1 cut(s) 559
EcoRII CCWGG 3 cut(s) 86, 1031, 1191
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 998
FaeI CATG 6 cut(s) 193, 218, 314, 378, 517, 764
FalI AAGNNNNNCTT 2 cut(s) 673, 705
FaqI GGGAC 1 cut(s) 32
FatI CATG 6 cut(s) 189, 214, 310, 374, 513, 760
FauI CCCGC 1 cut(s) 57
FauNDI CATATG 1 cut(s) 106
FbaI TGATCA 2 cut(s) 186, 250
Fnu4HI GCNGC 8 cut(s) 26, 29, 32, 53, 78, 428, 685, 948
FokI GGATG 1 cut(s) 103
FriOI GRGCYC 1 cut(s) 998
Fsp4HI GCNGC 8 cut(s) 26, 29, 32, 53, 78, 428, 685, 948
FspBI CTAG 3 cut(s) 726, 1013, 1235
GluI GCNGC 8 cut(s) 26, 29, 32, 53, 78, 428, 685, 948
GsaI CCCAGC 1 cut(s) 1221
HaeIII GGCC 2 cut(s) 996, 1228
HapII CCGG 1 cut(s) 422
Hin1II CATG 6 cut(s) 193, 218, 314, 378, 517, 764
HincII GTYRAC 1 cut(s) 1173
HindII GTYRAC 1 cut(s) 1173
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 5 cut(s) 98, 694, 887, 929, 1238
HpaII CCGG 1 cut(s) 422
HphI GGTGA 2 cut(s) 571, 793
Hpy166II GTNNAC 4 cut(s) 245, 331, 741, 1173
Hpy188I TCNGA 6 cut(s) 352, 357, 451, 880, 1073, 1130
Hpy188III TCNNGA 4 cut(s) 149, 311, 716, 726
Hpy8I GTNNAC 4 cut(s) 245, 331, 741, 1173
HpyAV CCTTC 3 cut(s) 976, 1053, 1193
HpyCH4III ACNGT 2 cut(s) 511, 1103
HpyCH4V TGCA 6 cut(s) 230, 284, 364, 584, 592, 950
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 40, 49, 598, 1002
HpyF3I CTNAG 6 cut(s) 261, 349, 450, 484, 915, 1001
Hsp92II CATG 6 cut(s) 193, 218, 314, 378, 517, 764
Ksp22I TGATCA 2 cut(s) 186, 250
Kzo9I GATC 7 cut(s) 186, 250, 313, 352, 910, 1075, 1153
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 4 cut(s) 22, 404, 503, 1186
Lsp1109I GCAGC 3 cut(s) 414, 696, 934
LweI GCATC 2 cut(s) 959, 1221
MaeI CTAG 3 cut(s) 726, 1013, 1235
MaeIII GTNAC 1 cut(s) 559
MalI GATC 7 cut(s) 188, 252, 315, 354, 912, 1077, 1155
MbiI CCGCTC 2 cut(s) 55, 80
MboI GATC 7 cut(s) 186, 250, 313, 352, 910, 1075, 1153
MboII GAAGA 4 cut(s) 49, 328, 409, 809
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 2 cut(s) 352, 910
MhlI GDGCHC 3 cut(s) 45, 998, 1191
MlyI GAGTC 3 cut(s) 107, 703, 1247
MmeI TCCRAC 1 cut(s) 75
Mph1103I ATGCAT 1 cut(s) 286
MroXI GAANNNNTTC 1 cut(s) 11
MseI TTAA 2 cut(s) 641, 1026
MslI CAYNNNNRTG 3 cut(s) 89, 326, 512
MspA1I CMGCKG 2 cut(s) 294, 1067
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 4 cut(s) 88, 422, 1033, 1193
MunI CAATTG 1 cut(s) 498
Mva1269I GAATGC 2 cut(s) 286, 1110
MvaI CCWGG 3 cut(s) 88, 1033, 1193
MwoI GCNNNNNNNGC 5 cut(s) 31, 40, 49, 598, 1002
NciI CCSGG 1 cut(s) 422
NdeI CATATG 1 cut(s) 106
NdeII GATC 7 cut(s) 186, 250, 313, 352, 910, 1075, 1153
NheI GCTAGC 1 cut(s) 1012
NlaIII CATG 6 cut(s) 193, 218, 314, 378, 517, 764
NlaIV GGNNCC 3 cut(s) 24, 505, 996
NmuCI GTSAC 1 cut(s) 559
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 2 cut(s) 378, 764
OliI CACNNNNGTG 1 cut(s) 326
PaeR7I CTCGAG 1 cut(s) 716
PagI TCATGA 1 cut(s) 310
PaqCI CACCTGC 1 cut(s) 575
PciI ACATGT 1 cut(s) 760
PciSI GCTCTTC 1 cut(s) 62
PctI GAATGC 2 cut(s) 286, 1110
PdmI GAANNNNTTC 1 cut(s) 11
PfeI GAWTC 2 cut(s) 887, 929
PflMI CCANNNNNTGG 2 cut(s) 395, 608
PkrI GCNGC 8 cut(s) 27, 30, 33, 54, 79, 429, 686, 949
PleI GAGTC 3 cut(s) 106, 702, 1246
PpsI GAGTC 3 cut(s) 106, 702, 1246
PscI ACATGT 1 cut(s) 760
Psp6I CCWGG 3 cut(s) 86, 1031, 1191
PspEI GGTNACC 1 cut(s) 559
PspFI CCCAGC 1 cut(s) 1217
PspGI CCWGG 3 cut(s) 86, 1031, 1191
PspN4I GGNNCC 3 cut(s) 24, 505, 996
PspOMI GGGCCC 1 cut(s) 994
PspPI GGNCC 5 cut(s) 242, 331, 769, 994, 995
PstI CTGCAG 1 cut(s) 586
PsuI RGATCY 2 cut(s) 352, 910
PvuII CAGCTG 1 cut(s) 1067
RsaI GTAC 1 cut(s) 1272
RsaNI GTAC 1 cut(s) 1271
RseI CAYNNNNRTG 3 cut(s) 89, 326, 512
SapI GCTCTTC 1 cut(s) 62
SaqAI TTAA 2 cut(s) 641, 1026
SatI GCNGC 8 cut(s) 26, 29, 32, 53, 78, 428, 685, 948
Sau3AI GATC 7 cut(s) 186, 250, 313, 352, 910, 1075, 1153
Sau96I GGNCC 5 cut(s) 242, 331, 769, 994, 995
SchI GAGTC 3 cut(s) 107, 703, 1247
ScrFI CCNGG 4 cut(s) 88, 422, 1033, 1193
SduI GDGCHC 3 cut(s) 45, 998, 1191
SfaNI GCATC 2 cut(s) 959, 1221
SfcI CTRYAG 1 cut(s) 582
Sfr274I CTCGAG 1 cut(s) 716
SinI GGWCC 3 cut(s) 242, 331, 769
SlaI CTCGAG 1 cut(s) 716
SmiI ATTTAAAT 1 cut(s) 1027
SmiMI CAYNNNNRTG 3 cut(s) 89, 326, 512
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
SsiI CCGC 8 cut(s) 26, 29, 32, 50, 53, 68, 78, 292
SspMI CTAG 3 cut(s) 726, 1013, 1235
StyD4I CCNGG 4 cut(s) 86, 420, 1031, 1191
SwaI ATTTAAAT 1 cut(s) 1027
TaaI ACNGT 2 cut(s) 511, 1103
TaqI TCGA 5 cut(s) 15, 60, 717, 795, 831
TauI GCSGC 5 cut(s) 28, 31, 34, 55, 80
TfiI GAWTC 2 cut(s) 887, 929
Tru1I TTAA 2 cut(s) 641, 1026
Tru9I TTAA 2 cut(s) 641, 1026
TscAI CASTG 1 cut(s) 748
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 3 cut(s) 427, 684, 947
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 2 cut(s) 299, 900
TspRI CASTG 1 cut(s) 748
Van91I CCANNNNNTGG 2 cut(s) 395, 608
VpaK11BI GGWCC 3 cut(s) 242, 331, 769
XapI RAATTY 6 cut(s) 7, 392, 605, 1159, 1258, 1299
XbaI TCTAGA 1 cut(s) 725
XceI RCATGY 2 cut(s) 378, 764
XcmI CCANNNNNNNNNTGG 1 cut(s) 549
XhoI CTCGAG 1 cut(s) 716
XmnI GAANNNNTTC 1 cut(s) 11
XspI CTAG 3 cut(s) 726, 1013, 1235
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.