Rroxscaffold_2G00080510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012020
Physical Location & Seq
Reverse (-)
3486203 .. 3491199
4997 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00080510.1

Sequence Viewer

Length: 1185 bp
ATGGGGAATTTGTTCGACAAGGAGCCGCCGCCGCCTATTGTGCTCGTCCCGCCGCTCTTCGATTATCCGCCTCTCGCCGCTCGCACCAGGATGTTGGAGTCGTCATATGACTTGTTGTTTGGGAAGCTTGCTTTGAAAGGTCTGTTTCAGGACTATTTTGAACAAGCAAGGCATTTCAGCACAATGATCATGCTAAAGCCGATTGATGACCCTCATGTGGATTTAGTTGCAACGCTCTCTGGTCCACTTGATCACAAACCTGAGGAGACCATTGTTGGGAATGCATTTTTCCGCTGGCAAAGTGATGTTCATGATCCTCACACATTTGTGGACCTCTTCGTGTCAAACTCAGATCCGATTTTGCAGACGAGGGCATGTGCTTACTACCCAAAATTTGGATTTGGAGCATTTGGTGTTTTCCCGGTGCTGCTAAAACAAAGAATAACATCTGAGGACTTTGGTGTTATGGGATTGAGATACGGCTCAGCAAACTTATCAATTGGAGCCACAGTCATGCCTTTCTCTGTGAAAGATGATTTGCCAAGAAAGGCATGGTTGGTGACCAAAATGGGAAGTCTAACTGCAGGTGTGCAGTATGAGCCAGAATTTCACTGCCTCGTTCTATCAACATGTGGTGGTCCAAAGACGGGTGAAAAACCCCTCGAAGAAAATCAAGTTGTTGGGATTACGAATTATATCGATTTTGGCTTTGAATTACAATCAAGGGTTGATAGGTTTGATGATACCAAAACATCAGACGATGAATCAAACTCAAACAAAGTACCAGATCCTAAGAATATACCAGATTCCACCTTTCAAATAGCTGCATCTTGGCAAGCCAACAAGAACTTTTTGCTGAAGGGAAAGGTGGGCCCTCTCAGCTCATCGCTAGCATGGGCATTCAAATCATGGTGGAAACCTTCTTTCACATTCAGCATTTCAGCTGTCAGAGATCACGTATTTGGAAATACAGCTTATGGTTTTGGCATTCGTGTTGAGAATATCAGACAAGCCAGCTACCAAAGAGCCGATCCAAATTTTGTTATGTTGACACCAAGTAAGGAGCACCTGGCACAAGGCATTGTTTGGCAAGCTGGGAAACGGCCGATGCTGGAGTCAAATATAAATGCTGGAAATTTTGACGGCGTACCAATGGAATTACAACCCTTGCGAAAAATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

394

Amino Acids

44.05

Weight (kDa)

6.09

Isoelectric Point (pI)

35.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G27930 AT3G27930 AT3G27930
fragaria_vesca FvH4_6g50760
malus_domestica MD09G1032600.v1.1 MD17G1034200.v1.1
prunus_persica Prupe.3G285400_v2.0.a1
pyrus_communis pycom17g03160
rosa_chinensis RchiOBHm_Chr2g0171701
rosa_laevigata RLG00000022030
rosa_multiflora Rmu_ssc0000252.1_g000012
rosa_roxburghii Rroxscaffold_2G00080510
rosa_rugosa Rorug02G0555400
rosa_samantha Rh2BG641000 Rh2BG643500 Rh2CG609200 Rh2DG651900 Rh2DG657200
rosa_wichuraiana Rw2G052180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 575
Acc36I ACCTGC 1 cut(s) 575
AccB7I CCANNNNNTGG 1 cut(s) 395
AccBSI CCGCTC 2 cut(s) 55, 80
AciI CCGC 8 cut(s) 26, 29, 32, 50, 53, 68, 78, 292
AclWI GGATC 4 cut(s) 308, 347, 782, 1025
AcoI YGGCCR 1 cut(s) 1103
AcsI RAATTY 6 cut(s) 7, 392, 605, 1036, 1135, 1176
AcuI CTGAAG 1 cut(s) 878
AfaI GTAC 2 cut(s) 783, 1149
AfiI CCNNNNNNNGG 4 cut(s) 217, 276, 395, 647
AflIII ACRYGT 1 cut(s) 629
AgsI TTSAA 5 cut(s) 136, 161, 713, 818, 904
AjnI CCWGG 2 cut(s) 86, 1068
AleI CACNNNNGTG 1 cut(s) 326
AluBI AGCT 7 cut(s) 127, 824, 882, 944, 974, 1017, 1094
AluI AGCT 7 cut(s) 127, 824, 882, 944, 974, 1017, 1094
Alw21I GWGCWC 2 cut(s) 45, 1068
Alw26I GTCTC 1 cut(s) 260
AlwI GGATC 4 cut(s) 308, 347, 782, 1025
AoxI GGCC 2 cut(s) 871, 1103
ApaI GGGCCC 1 cut(s) 875
ApeKI GCWGC 2 cut(s) 427, 824
ApoI RAATTY 6 cut(s) 7, 392, 605, 1036, 1135, 1176
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
Asp700I GAANNNNTTC 1 cut(s) 11
AspS9I GGNCC 5 cut(s) 242, 331, 638, 871, 872
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 2 cut(s) 571, 662
AsuNHI GCTAGC 1 cut(s) 889
AvaII GGWCC 3 cut(s) 242, 331, 638
AxyI CCTNAGG 1 cut(s) 261
BaeGI GKGCMC 1 cut(s) 875
BanII GRGCYC 1 cut(s) 875
Bbv12I GWGCWC 2 cut(s) 45, 1068
BbvI GCAGC 2 cut(s) 414, 811
BceAI ACGGC 3 cut(s) 496, 1118, 1159
BciT130I CCWGG 2 cut(s) 88, 1070
BclI TGATCA 2 cut(s) 186, 250
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 260
BfaI CTAG 1 cut(s) 890
BfmI CTRYAG 1 cut(s) 582
BfuAI ACCTGC 1 cut(s) 575
BisI GCNGC 7 cut(s) 26, 29, 32, 53, 78, 428, 825
BlpI GCTNAGC 1 cut(s) 484
BlsI GCNGC 7 cut(s) 27, 30, 33, 54, 79, 429, 826
Bme1390I CCNGG 3 cut(s) 88, 422, 1070
Bme18I GGWCC 3 cut(s) 242, 331, 638
BmgT120I GGNCC 5 cut(s) 242, 331, 638, 871, 872
BmiI GGNNCC 3 cut(s) 24, 505, 873
BmrFI CCNGG 3 cut(s) 88, 422, 1070
BmsI GCATC 2 cut(s) 836, 1098
BmtI GCTAGC 1 cut(s) 893
BpmI CTGGAG 1 cut(s) 1133
Bpu1102I GCTNAGC 1 cut(s) 484
BpuMI CCSGG 1 cut(s) 422
Bsa29I ATCGAT 1 cut(s) 699
BsaAI YACGTR 1 cut(s) 958
BsaI GGTCTC 1 cut(s) 260
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 4 cut(s) 217, 276, 395, 647
Bse21I CCTNAGG 1 cut(s) 261
BseBI CCWGG 2 cut(s) 88, 1070
BseCI ATCGAT 1 cut(s) 699
BseGI GGATG 1 cut(s) 96
BseLI CCNNNNNNNGG 4 cut(s) 217, 276, 395, 647
BseMII CTCAG 5 cut(s) 252, 363, 441, 498, 892
BseRI GAGGAG 1 cut(s) 278
BseSI GKGCMC 1 cut(s) 875
BseX3I CGGCCG 1 cut(s) 1103
BseXI GCAGC 2 cut(s) 414, 811
BseYI CCCAGC 1 cut(s) 1094
BsgI GTGCAG 1 cut(s) 611
Bsh1285I CGRYCG 1 cut(s) 1106
BshFI GGCC 2 cut(s) 873, 1105
BshVI ATCGAT 1 cut(s) 699
BsiEI CGRYCG 1 cut(s) 1106
BsiHKAI GWGCWC 2 cut(s) 45, 1068
BsiSI CCGG 1 cut(s) 422
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 4 cut(s) 217, 276, 395, 647
BsmAI GTCTC 1 cut(s) 260
BsmFI GGGAC 1 cut(s) 32
BsmI GAATGC 3 cut(s) 286, 899, 987
BsnI GGCC 2 cut(s) 873, 1105
Bso31I GGTCTC 1 cut(s) 260
Bsp120I GGGCCC 1 cut(s) 871
Bsp1286I GDGCHC 3 cut(s) 45, 875, 1068
Bsp143I GATC 7 cut(s) 186, 250, 313, 352, 787, 952, 1030
Bsp1720I GCTNAGC 1 cut(s) 484
BspACI CCGC 8 cut(s) 26, 29, 32, 50, 53, 68, 78, 292
BspANI GGCC 2 cut(s) 873, 1105
BspCNI CTCAG 5 cut(s) 253, 362, 442, 497, 891
BspDI ATCGAT 1 cut(s) 699
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 3 cut(s) 24, 505, 873
BspMAI CTGCAG 1 cut(s) 586
BspMI ACCTGC 1 cut(s) 575
BspOI GCTAGC 1 cut(s) 893
BspPI GGATC 4 cut(s) 308, 347, 782, 1025
BspQI GCTCTTC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 260
BsrBI CCGCTC 2 cut(s) 55, 80
BssMI GATC 7 cut(s) 186, 250, 313, 352, 787, 952, 1030
Bst2UI CCWGG 2 cut(s) 88, 1070
Bst4CI ACNGT 1 cut(s) 511
Bst6I CTCTTC 2 cut(s) 62, 341
BstBAI YACGTR 1 cut(s) 958
BstC8I GCNNGC 7 cut(s) 82, 129, 296, 837, 891, 1015, 1092
BstDEI CTNAG 6 cut(s) 261, 349, 450, 484, 792, 878
BstEII GGTNACC 1 cut(s) 559
BstF5I GGATG 1 cut(s) 96
BstKTI GATC 7 cut(s) 189, 253, 316, 355, 790, 955, 1033
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 7 cut(s) 186, 250, 313, 352, 787, 952, 1030
BstMCI CGRYCG 1 cut(s) 1106
BstMWI GCNNNNNNNGC 5 cut(s) 31, 40, 49, 598, 879
BstNI CCWGG 2 cut(s) 88, 1070
BstNSI RCATGY 2 cut(s) 378, 633
BstPI GGTNACC 1 cut(s) 559
BstSCI CCNGG 3 cut(s) 86, 420, 1068
BstSFI CTRYAG 1 cut(s) 582
BstSLI GKGCMC 1 cut(s) 875
BstV1I GCAGC 2 cut(s) 414, 811
BstX2I RGATCY 2 cut(s) 352, 787
BstXI CCANNNNNNTGG 1 cut(s) 94
BstYI RGATCY 2 cut(s) 352, 787
BstZI CGGCCG 1 cut(s) 1103
Bsu15I ATCGAT 1 cut(s) 699
Bsu36I CCTNAGG 1 cut(s) 261
BsuRI GGCC 2 cut(s) 873, 1105
BsuTUI ATCGAT 1 cut(s) 699
BtgZI GCGATG 1 cut(s) 870
BtsCI GGATG 1 cut(s) 96
BtsI GCAGTG 1 cut(s) 610
BtsIMutI CAGTG 1 cut(s) 610
BveI ACCTGC 1 cut(s) 575
Cac8I GCNNGC 7 cut(s) 82, 129, 296, 837, 891, 1015, 1092
CciI TCATGA 1 cut(s) 310
Cfr13I GGNCC 5 cut(s) 242, 331, 638, 871, 872
ClaI ATCGAT 1 cut(s) 699
Csp6I GTAC 2 cut(s) 782, 1148
CviAII CATG 9 cut(s) 190, 215, 311, 375, 514, 552, 630, 894, 909
CviQI GTAC 2 cut(s) 782, 1148
DdeI CTNAG 6 cut(s) 261, 349, 450, 484, 792, 878
DpnI GATC 7 cut(s) 188, 252, 315, 354, 789, 954, 1032
DpnII GATC 7 cut(s) 186, 250, 313, 352, 787, 952, 1030
EaeI YGGCCR 1 cut(s) 1103
EagI CGGCCG 1 cut(s) 1103
Eam1104I CTCTTC 2 cut(s) 62, 341
EarI CTCTTC 2 cut(s) 62, 341
EciI GGCGGA 1 cut(s) 57
EclXI CGGCCG 1 cut(s) 1103
Eco24I GRGCYC 1 cut(s) 875
Eco31I GGTCTC 1 cut(s) 260
Eco47I GGWCC 3 cut(s) 242, 331, 638
Eco52I CGGCCG 1 cut(s) 1103
Eco57I CTGAAG 1 cut(s) 878
Eco81I CCTNAGG 1 cut(s) 261
Eco91I GGTNACC 1 cut(s) 559
EcoO109I RGGNCCY 1 cut(s) 872
EcoO65I GGTNACC 1 cut(s) 559
EcoRII CCWGG 2 cut(s) 86, 1068
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 875
FaeI CATG 9 cut(s) 193, 218, 314, 378, 517, 555, 633, 897, 912
FaqI GGGAC 1 cut(s) 32
FatI CATG 9 cut(s) 189, 214, 310, 374, 513, 551, 629, 893, 908
FauI CCCGC 1 cut(s) 57
FauNDI CATATG 1 cut(s) 106
FbaI TGATCA 2 cut(s) 186, 250
Fnu4HI GCNGC 7 cut(s) 26, 29, 32, 53, 78, 428, 825
FokI GGATG 1 cut(s) 103
FriOI GRGCYC 1 cut(s) 875
Fsp4HI GCNGC 7 cut(s) 26, 29, 32, 53, 78, 428, 825
FspBI CTAG 1 cut(s) 890
GluI GCNGC 7 cut(s) 26, 29, 32, 53, 78, 428, 825
GsaI CCCAGC 1 cut(s) 1098
GsuI CTGGAG 1 cut(s) 1133
HaeIII GGCC 2 cut(s) 873, 1105
HapII CCGG 1 cut(s) 422
Hin1II CATG 9 cut(s) 193, 218, 314, 378, 517, 555, 633, 897, 912
HincII GTYRAC 1 cut(s) 1050
HindII GTYRAC 1 cut(s) 1050
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 4 cut(s) 98, 764, 806, 1115
HpaII CCGG 1 cut(s) 422
HphI GGTGA 2 cut(s) 571, 662
Hpy166II GTNNAC 3 cut(s) 245, 331, 1050
Hpy188I TCNGA 6 cut(s) 352, 357, 451, 757, 950, 1007
Hpy188III TCNNGA 2 cut(s) 149, 311
Hpy8I GTNNAC 3 cut(s) 245, 331, 1050
HpyAV CCTTC 2 cut(s) 853, 930
HpyCH4III ACNGT 1 cut(s) 511
HpyCH4IV ACGT 1 cut(s) 957
HpyCH4V TGCA 6 cut(s) 230, 284, 364, 584, 592, 827
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 40, 49, 598, 879
HpyF3I CTNAG 6 cut(s) 261, 349, 450, 484, 792, 878
HpySE526I ACGT 1 cut(s) 957
Hsp92II CATG 9 cut(s) 193, 218, 314, 378, 517, 555, 633, 897, 912
Ksp22I TGATCA 2 cut(s) 186, 250
Kzo9I GATC 7 cut(s) 186, 250, 313, 352, 787, 952, 1030
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 4 cut(s) 22, 404, 503, 1063
Lsp1109I GCAGC 2 cut(s) 414, 811
LweI GCATC 2 cut(s) 836, 1098
MaeI CTAG 1 cut(s) 890
MaeII ACGT 1 cut(s) 957
MaeIII GTNAC 1 cut(s) 559
MalI GATC 7 cut(s) 188, 252, 315, 354, 789, 954, 1032
MbiI CCGCTC 2 cut(s) 55, 80
MboI GATC 7 cut(s) 186, 250, 313, 352, 787, 952, 1030
MboII GAAGA 3 cut(s) 49, 328, 677
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 2 cut(s) 352, 787
MhlI GDGCHC 3 cut(s) 45, 875, 1068
MlyI GAGTC 2 cut(s) 107, 1124
MmeI TCCRAC 1 cut(s) 75
Mph1103I ATGCAT 1 cut(s) 286
MroXI GAANNNNTTC 1 cut(s) 11
MslI CAYNNNNRTG 3 cut(s) 89, 326, 512
MspA1I CMGCKG 2 cut(s) 294, 944
MspI CCGG 1 cut(s) 422
MspR9I CCNGG 3 cut(s) 88, 422, 1070
MunI CAATTG 1 cut(s) 498
Mva1269I GAATGC 3 cut(s) 286, 899, 987
MvaI CCWGG 2 cut(s) 88, 1070
MwoI GCNNNNNNNGC 5 cut(s) 31, 40, 49, 598, 879
NciI CCSGG 1 cut(s) 422
NdeI CATATG 1 cut(s) 106
NdeII GATC 7 cut(s) 186, 250, 313, 352, 787, 952, 1030
NheI GCTAGC 1 cut(s) 889
NlaIII CATG 9 cut(s) 193, 218, 314, 378, 517, 555, 633, 897, 912
NlaIV GGNNCC 3 cut(s) 24, 505, 873
NmuCI GTSAC 1 cut(s) 559
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 2 cut(s) 378, 633
OliI CACNNNNGTG 1 cut(s) 326
PagI TCATGA 1 cut(s) 310
PaqCI CACCTGC 1 cut(s) 575
PciI ACATGT 1 cut(s) 629
PciSI GCTCTTC 1 cut(s) 62
PctI GAATGC 3 cut(s) 286, 899, 987
PdmI GAANNNNTTC 1 cut(s) 11
PfeI GAWTC 2 cut(s) 764, 806
PflMI CCANNNNNTGG 1 cut(s) 395
PkrI GCNGC 7 cut(s) 27, 30, 33, 54, 79, 429, 826
PleI GAGTC 2 cut(s) 106, 1123
PpsI GAGTC 2 cut(s) 106, 1123
Ppu21I YACGTR 1 cut(s) 958
PscI ACATGT 1 cut(s) 629
Psp6I CCWGG 2 cut(s) 86, 1068
PspEI GGTNACC 1 cut(s) 559
PspFI CCCAGC 1 cut(s) 1094
PspGI CCWGG 2 cut(s) 86, 1068
PspN4I GGNNCC 3 cut(s) 24, 505, 873
PspOMI GGGCCC 1 cut(s) 871
PspPI GGNCC 5 cut(s) 242, 331, 638, 871, 872
PstI CTGCAG 1 cut(s) 586
PsuI RGATCY 2 cut(s) 352, 787
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 2 cut(s) 783, 1149
RsaNI GTAC 2 cut(s) 782, 1148
RseI CAYNNNNRTG 3 cut(s) 89, 326, 512
SapI GCTCTTC 1 cut(s) 62
SatI GCNGC 7 cut(s) 26, 29, 32, 53, 78, 428, 825
Sau3AI GATC 7 cut(s) 186, 250, 313, 352, 787, 952, 1030
Sau96I GGNCC 5 cut(s) 242, 331, 638, 871, 872
SchI GAGTC 2 cut(s) 107, 1124
ScrFI CCNGG 3 cut(s) 88, 422, 1070
SduI GDGCHC 3 cut(s) 45, 875, 1068
SfaNI GCATC 2 cut(s) 836, 1098
SfcI CTRYAG 1 cut(s) 582
SinI GGWCC 3 cut(s) 242, 331, 638
SmiMI CAYNNNNRTG 3 cut(s) 89, 326, 512
SsiI CCGC 8 cut(s) 26, 29, 32, 50, 53, 68, 78, 292
SspMI CTAG 1 cut(s) 890
StyD4I CCNGG 3 cut(s) 86, 420, 1068
TaaI ACNGT 1 cut(s) 511
TaiI ACGT 1 cut(s) 960
TaqI TCGA 4 cut(s) 15, 60, 663, 699
TauI GCSGC 5 cut(s) 28, 31, 34, 55, 80
TfiI GAWTC 2 cut(s) 764, 806
TscAI CASTG 1 cut(s) 617
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 2 cut(s) 427, 824
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 2 cut(s) 299, 777
TspRI CASTG 1 cut(s) 617
Van91I CCANNNNNTGG 1 cut(s) 395
VpaK11BI GGWCC 3 cut(s) 242, 331, 638
XapI RAATTY 6 cut(s) 7, 392, 605, 1036, 1135, 1176
XceI RCATGY 2 cut(s) 378, 633
XcmI CCANNNNNNNNNTGG 1 cut(s) 549
XmnI GAANNNNTTC 1 cut(s) 11
XspI CTAG 1 cut(s) 890
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.