MD09G1034900.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011710
Physical Location & Seq
Forward (+)
2129274 .. 2132937
3664 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1034900.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGAAGGAGAAGAGCGACGGCGGCGGAGGCGGAGGCGGGTATTTGAGGGCGGATCAGATAGATCTGAAGAGCTTGGACGAGCAGCTGCAGCGGCACCTCAGCAGGGCATGTACGATGGAGAAGAACAAGAAGCCGGAGGAAGACGAAGAGGCAGTGGCGGCGTCGAGGAGCAACACTCCCAGAAGGCAAGAGTGGGAGATTGATCCCTCCAAGCTCGTCGTCAAGGGCGCTATAGCTCGTGGCACTTTCGGCGCGGTTCACCGTGGCATGTACGACGGCCAAGACGTCGCCGTTAAACTTCTAGATTGGGGAGAAGAGGGGCATAGATCAGATGCTGAAATAGCTTCACTAAGAGCAGCTTTTACTCAAGAAGTTGCTGTCTGGCATAAGCTTGAGCACCCTAATGTAACCAAGTTTATAGGGGCTACAATGGGATCATCGGAGCTACACATACAAACAGATAATGGCCAAGTTGGCATGCCAAGTAATGTTTGTTGTGTTGTTGTTGAATATTGTCCTGGCGGTGCACTGAAATCTTACCTCATAAAGAACCGCAGAAGGAAGCTGGCATTCAAACTAGTTGTCCAACTGGCATTGGATCTTGCAAGAGGTTTGTGCTATCTTCATTCGAAGAAGATTGTTCACAGAGATGTTAAAACAGAAAACATGCTTCTGGACAAGACACGAACTGTAAAAATAGCCGATTTTGGGGTTGCTCGTGTTGAGGCTTCAAATCCTAATGAAATGACTGGGGAAACTGGAACCCTCGGTTACATGGCTCCAGAGGTTCTAAATGGCCACCCATATAACAGAAAATGTGATGTGTACAGTTTCGGAATCTGCTTGTGGGAAATATACTGTTGTGACATGCCGTATCCTGATCTCAGCTTCTCGGAAGTGACATCAGCCGTTGTCCGCCAGAATCTGAGGCCAGAGATACCTCGTTGTTGCCCAAGTGCTCTTGCAAATGTGATGAAGCGATGTTGGGACGCTAGCCCTGACAAGCGGCCTGAGATGGACGAGGTGGTGGCCATGTTGGAGGCCATTGACACATCTAAAGGTGGAGGTATGATCCCCGGGGACCAGCCGCAGGGTTGTTTCTGTTTCCTCAAGTACCGCGGACCTTGA

Protein Analysis

376

Amino Acids

41.55

Weight (kDa)

6.76

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 75 - 346 3.7e-61 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 75 - 344 3.1e-48 Protein kinase domain
Kinase-like PF14531 184 - 292 1.3e-06 Kinase-like
Pkinase_fungal PF17667 204 - 278 7.1e-06 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011710)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01490 AT3G01490
fragaria_vesca FvH4_6g50600 FvH4_6g50600
malus_domestica MD09G1034900.v1.1
prunus_persica Prupe.3G283600_v2.0.a1 Prupe.3G283600_v2.0.a1
pyrus_communis pycom111g02780
rosa_chinensis RchiOBHm_Chr2g0171391
rosa_laevigata RLG00000022009
rosa_multiflora Rmu_sc0007646.1_g000009
rosa_roxburghii Rroxscaffold_2G00080700
rosa_rugosa Rorug02G0553600 Rorug02G0553700
rosa_samantha Rh2AG628100 Rh2BG638500 Rh2DG649500 Rh2DG655600
rosa_wichuraiana Rw2G052020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 288
AccB1I GGYRCC 1 cut(s) 93
AccII CGCG 2 cut(s) 254, 1119
AclWI GGATC 5 cut(s) 60, 197, 442, 606, 1066
AcoI YGGCCR 4 cut(s) 277, 466, 796, 1029
AcuI CTGAAG 1 cut(s) 86
AcyI GRCGYC 2 cut(s) 161, 285
AfaI GTAC 4 cut(s) 112, 272, 827, 1115
AfiI CCNNNNNNNGG 3 cut(s) 103, 708, 1090
AgsI TTSAA 3 cut(s) 509, 574, 732
AhlI ACTAGT 1 cut(s) 577
AjnI CCWGG 1 cut(s) 517
Alw21I GWGCWC 3 cut(s) 399, 529, 961
Alw44I GTGCAC 1 cut(s) 525
AlwI GGATC 5 cut(s) 60, 197, 442, 606, 1066
AlwNI CAGNNNCTG 2 cut(s) 335, 925
Ama87I CYCGRG 1 cut(s) 1076
AoxI GGCC 7 cut(s) 277, 466, 796, 929, 1007, 1029, 1041
ApaLI GTGCAC 1 cut(s) 525
ApeKI GCWGC 4 cut(s) 82, 85, 88, 356
ArsI GACNNNNNNTTYG 2 cut(s) 567, 599
AspLEI GCGC 2 cut(s) 230, 254
AspS9I GGNCC 2 cut(s) 1081, 1121
AsuC2I CCSGG 2 cut(s) 1077, 1078
AsuHPI GGTGA 1 cut(s) 251
AsuII TTCGAA 1 cut(s) 629
AsuNHI GCTAGC 1 cut(s) 992
AvaI CYCGRG 1 cut(s) 1076
AvaII GGWCC 2 cut(s) 1081, 1121
BaeGI GKGCMC 1 cut(s) 529
BalI TGGCCA 3 cut(s) 468, 798, 1031
BanI GGYRCC 1 cut(s) 93
BauI CACGAG 2 cut(s) 237, 717
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 3 cut(s) 399, 529, 961
BbvCI CCTCAGC 1 cut(s) 98
BbvI GCAGC 4 cut(s) 72, 94, 100, 368
BccI CCATC 2 cut(s) 109, 1009
BceAI ACGGC 5 cut(s) 34, 275, 292, 856, 893
BciT130I CCWGG 1 cut(s) 519
BciVI GTATCC 1 cut(s) 885
BcnI CCSGG 2 cut(s) 1077, 1078
BcuI ACTAGT 1 cut(s) 577
BfaI CTAG 3 cut(s) 302, 578, 993
BfmI CTRYAG 2 cut(s) 86, 231
BfoI RGCGCY 1 cut(s) 231
BfuI GTATCC 1 cut(s) 885
BglI GCCNNNNNGGC 1 cut(s) 474
BglII AGATCT 1 cut(s) 61
BisI GCNGC 9 cut(s) 22, 83, 86, 89, 92, 159, 357, 1007, 1088
BlsI GCNGC 9 cut(s) 23, 84, 87, 90, 93, 160, 358, 1008, 1089
Bme1390I CCNGG 3 cut(s) 519, 1077, 1078
Bme18I GGWCC 2 cut(s) 1081, 1121
BmeT110I CYCGRG 1 cut(s) 1076
BmgT120I GGNCC 2 cut(s) 1081, 1121
BmiI GGNNCC 4 cut(s) 95, 763, 780, 1082
BmrFI CCNGG 3 cut(s) 519, 1077, 1078
BmrI ACTGGG 1 cut(s) 759
BmsI GCATC 1 cut(s) 322
BmtI GCTAGC 1 cut(s) 996
BmuI ACTGGG 1 cut(s) 759
BpiI GAAGAC 1 cut(s) 147
BplI GAGNNNNNCTC 2 cut(s) 160, 192
BpmI CTGGAG 1 cut(s) 765
Bpu10I CCTNAGC 1 cut(s) 98
Bpu14I TTCGAA 1 cut(s) 629
BpuEI CTTGAG 3 cut(s) 351, 413, 1094
BpuMI CCSGG 2 cut(s) 1077, 1078
BsaHI GRCGYC 2 cut(s) 161, 285
BsaJI CCNNGG 6 cut(s) 262, 766, 1075, 1076, 1077, 1117
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 708, 1090
Bse1I ACTGG 3 cut(s) 594, 754, 763
BseBI CCWGG 1 cut(s) 519
BseDI CCNNGG 6 cut(s) 262, 766, 1075, 1076, 1077, 1117
BseLI CCNNNNNNNGG 3 cut(s) 103, 708, 1090
BseMII CTCAG 4 cut(s) 112, 898, 917, 1002
BseNI ACTGG 3 cut(s) 594, 754, 763
BseRI GAGGAG 1 cut(s) 181
BseSI GKGCMC 1 cut(s) 529
BseXI GCAGC 4 cut(s) 72, 94, 100, 368
Bsh1236I CGCG 2 cut(s) 254, 1119
BshFI GGCC 7 cut(s) 279, 468, 798, 931, 1009, 1031, 1043
BshNI GGYRCC 1 cut(s) 93
BsiHKAI GWGCWC 3 cut(s) 399, 529, 961
BsiHKCI CYCGRG 1 cut(s) 1076
BsiSI CCGG 2 cut(s) 134, 1077
BslFI GGGAC 2 cut(s) 1001, 1094
BslI CCNNNNNNNGG 3 cut(s) 103, 708, 1090
BsmFI GGGAC 2 cut(s) 1001, 1094
BsmI GAATGC 1 cut(s) 569
BsnI GGCC 7 cut(s) 279, 468, 798, 931, 1009, 1031, 1043
BsoBI CYCGRG 1 cut(s) 1076
Bsp119I TTCGAA 1 cut(s) 629
Bsp1286I GDGCHC 3 cut(s) 399, 529, 961
Bsp1407I TGTACA 1 cut(s) 825
Bsp143I GATC 8 cut(s) 52, 61, 202, 326, 434, 598, 880, 1071
BspANI GGCC 7 cut(s) 279, 468, 798, 931, 1009, 1031, 1043
BspCNI CTCAG 4 cut(s) 111, 897, 918, 1003
BspFNI CGCG 2 cut(s) 254, 1119
BspLI GGNNCC 4 cut(s) 95, 763, 780, 1082
BspMAI CTGCAG 1 cut(s) 90
BspOI GCTAGC 1 cut(s) 996
BspPI GGATC 5 cut(s) 60, 197, 442, 606, 1066
BspQI GCTCTTC 2 cut(s) 5, 62
BspT104I TTCGAA 1 cut(s) 629
BspT107I GGYRCC 1 cut(s) 93
BsrGI TGTACA 1 cut(s) 825
BsrI ACTGG 3 cut(s) 594, 754, 763
BssECI CCNNGG 6 cut(s) 262, 766, 1075, 1076, 1077, 1117
BssMI GATC 8 cut(s) 52, 61, 202, 326, 434, 598, 880, 1071
BssNI GRCGYC 2 cut(s) 161, 285
BssSI CACGAG 2 cut(s) 237, 717
Bst2BI CACGAG 2 cut(s) 237, 717
Bst2UI CCWGG 1 cut(s) 519
Bst4CI ACNGT 4 cut(s) 263, 691, 830, 860
Bst6I CTCTTC 4 cut(s) 5, 62, 141, 309
BstACI GRCGYC 2 cut(s) 161, 285
BstAUI TGTACA 1 cut(s) 825
BstBI TTCGAA 1 cut(s) 629
BstC8I GCNNGC 3 cut(s) 479, 567, 994
BstDEI CTNAG 5 cut(s) 98, 350, 884, 926, 1011
BstDSI CCRYGG 2 cut(s) 262, 1117
BstFNI CGCG 2 cut(s) 254, 1119
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 2 cut(s) 230, 254
BstKTI GATC 8 cut(s) 55, 64, 205, 329, 437, 601, 883, 1074
BstMBI GATC 8 cut(s) 52, 61, 202, 326, 434, 598, 880, 1071
BstMWI GCNNNNNNNGC 8 cut(s) 21, 27, 88, 91, 158, 249, 341, 474
BstNI CCWGG 1 cut(s) 519
BstNSI RCATGY 5 cut(s) 111, 271, 481, 670, 871
BstSCI CCNGG 3 cut(s) 517, 1075, 1076
BstSFI CTRYAG 2 cut(s) 86, 231
BstSLI GKGCMC 1 cut(s) 529
BstUI CGCG 2 cut(s) 254, 1119
BstV1I GCAGC 4 cut(s) 72, 94, 100, 368
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 2 cut(s) 61, 598
BstYI RGATCY 2 cut(s) 61, 598
BsuI GTATCC 1 cut(s) 885
BsuRI GGCC 7 cut(s) 279, 468, 798, 931, 1009, 1031, 1043
BtgI CCRYGG 2 cut(s) 262, 1117
BtgZI GCGATG 1 cut(s) 994
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 2 cut(s) 159, 527
Cac8I GCNNGC 3 cut(s) 479, 567, 994
CaiI CAGNNNCTG 2 cut(s) 335, 925
CfoI GCGC 2 cut(s) 230, 254
Cfr13I GGNCC 2 cut(s) 1081, 1121
Cfr42I CCGCGG 1 cut(s) 1120
Cfr9I CCCGGG 1 cut(s) 1076
CseI GACGC 2 cut(s) 150, 998
Csp6I GTAC 4 cut(s) 111, 271, 826, 1114
CviAII CATG 7 cut(s) 108, 268, 478, 667, 775, 868, 1033
CviQI GTAC 4 cut(s) 111, 271, 826, 1114
DdeI CTNAG 5 cut(s) 98, 350, 884, 926, 1011
DpnI GATC 8 cut(s) 54, 63, 204, 328, 436, 600, 882, 1073
DpnII GATC 8 cut(s) 52, 61, 202, 326, 434, 598, 880, 1071
EaeI YGGCCR 4 cut(s) 277, 466, 796, 1029
Eam1104I CTCTTC 4 cut(s) 5, 62, 141, 309
EarI CTCTTC 4 cut(s) 5, 62, 141, 309
EciI GGCGGA 4 cut(s) 39, 45, 65, 905
Eco47I GGWCC 2 cut(s) 1081, 1121
Eco57I CTGAAG 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 1076
EcoRII CCWGG 1 cut(s) 517
FaeI CATG 7 cut(s) 111, 271, 481, 670, 778, 871, 1036
FalI AAGNNNNNCTT 2 cut(s) 343, 375
FaqI GGGAC 2 cut(s) 1001, 1094
FatI CATG 7 cut(s) 107, 267, 477, 666, 774, 867, 1032
FauI CCCGC 1 cut(s) 29
Fnu4HI GCNGC 9 cut(s) 22, 83, 86, 89, 92, 159, 357, 1007, 1088
Fsp4HI GCNGC 9 cut(s) 22, 83, 86, 89, 92, 159, 357, 1007, 1088
FspBI CTAG 3 cut(s) 302, 578, 993
GlaI GCGC 2 cut(s) 229, 253
GluI GCNGC 9 cut(s) 22, 83, 86, 89, 92, 159, 357, 1007, 1088
GsuI CTGGAG 1 cut(s) 765
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 7 cut(s) 279, 468, 798, 931, 1009, 1031, 1043
HapII CCGG 2 cut(s) 134, 1077
HgaI GACGC 2 cut(s) 150, 998
HhaI GCGC 2 cut(s) 230, 254
Hin1I GRCGYC 2 cut(s) 161, 285
Hin1II CATG 7 cut(s) 111, 271, 481, 670, 778, 871, 1036
Hin6I GCGC 2 cut(s) 228, 252
HinP1I GCGC 2 cut(s) 228, 252
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 2 cut(s) 837, 922
HpaII CCGG 2 cut(s) 134, 1077
HphI GGTGA 1 cut(s) 251
Hpy166II GTNNAC 4 cut(s) 259, 527, 643, 826
Hpy188I TCNGA 7 cut(s) 57, 66, 331, 442, 836, 895, 927
Hpy188III TCNNGA 5 cut(s) 302, 368, 674, 782, 878
Hpy8I GTNNAC 4 cut(s) 259, 527, 643, 826
Hpy99I CGWCG 5 cut(s) 20, 166, 221, 278, 290
HpyAV CCTTC 2 cut(s) 177, 552
HpyCH4III ACNGT 4 cut(s) 263, 691, 830, 860
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 4 cut(s) 88, 527, 605, 965
HpyF10VI GCNNNNNNNGC 8 cut(s) 21, 27, 88, 91, 158, 249, 341, 474
HpyF3I CTNAG 5 cut(s) 98, 350, 884, 926, 1011
HpySE526I ACGT 1 cut(s) 285
Hsp92I GRCGYC 2 cut(s) 161, 285
Hsp92II CATG 7 cut(s) 111, 271, 481, 670, 778, 871, 1036
HspAI GCGC 2 cut(s) 228, 252
KspI CCGCGG 1 cut(s) 1120
Kzo9I GATC 8 cut(s) 52, 61, 202, 326, 434, 598, 880, 1071
LguI GCTCTTC 2 cut(s) 5, 62
LmnI GCTCC 3 cut(s) 168, 442, 784
Lsp1109I GCAGC 4 cut(s) 72, 94, 100, 368
LweI GCATC 1 cut(s) 322
MaeI CTAG 3 cut(s) 302, 578, 993
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 4 cut(s) 406, 770, 863, 898
MalI GATC 8 cut(s) 54, 63, 204, 328, 436, 600, 882, 1073
MboI GATC 8 cut(s) 52, 61, 202, 326, 434, 598, 880, 1071
MboII GAAGA 9 cut(s) 22, 79, 133, 152, 158, 326, 614, 643, 646
MflI RGATCY 2 cut(s) 61, 598
MhlI GDGCHC 3 cut(s) 399, 529, 961
MlsI TGGCCA 3 cut(s) 468, 798, 1031
MluNI TGGCCA 3 cut(s) 468, 798, 1031
MmeI TCCRAC 2 cut(s) 610, 1017
Mox20I TGGCCA 3 cut(s) 468, 798, 1031
MscI TGGCCA 3 cut(s) 468, 798, 1031
MseI TTAA 2 cut(s) 294, 654
MslI CAYNNNNRTG 2 cut(s) 402, 648
Msp20I TGGCCA 3 cut(s) 468, 798, 1031
MspA1I CMGCKG 3 cut(s) 85, 91, 1119
MspI CCGG 2 cut(s) 134, 1077
MspR9I CCNGG 3 cut(s) 519, 1077, 1078
Mva1269I GAATGC 1 cut(s) 569
MvaI CCWGG 1 cut(s) 519
MvnI CGCG 2 cut(s) 254, 1119
MwoI GCNNNNNNNGC 8 cut(s) 21, 27, 88, 91, 158, 249, 341, 474
NciI CCSGG 2 cut(s) 1077, 1078
NdeII GATC 8 cut(s) 52, 61, 202, 326, 434, 598, 880, 1071
NheI GCTAGC 1 cut(s) 992
NlaIII CATG 7 cut(s) 111, 271, 481, 670, 778, 871, 1036
NlaIV GGNNCC 4 cut(s) 95, 763, 780, 1082
NmuCI GTSAC 2 cut(s) 863, 898
NspI RCATGY 5 cut(s) 111, 271, 481, 670, 871
NspV TTCGAA 1 cut(s) 629
PaeI GCATGC 1 cut(s) 481
PciSI GCTCTTC 2 cut(s) 5, 62
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PctI GAATGC 1 cut(s) 569
PfeI GAWTC 2 cut(s) 837, 922
PkrI GCNGC 9 cut(s) 23, 84, 87, 90, 93, 160, 358, 1008, 1089
Psp6I CCWGG 1 cut(s) 517
PspGI CCWGG 1 cut(s) 517
PspN4I GGNNCC 4 cut(s) 95, 763, 780, 1082
PspPI GGNCC 2 cut(s) 1081, 1121
PstI CTGCAG 1 cut(s) 90
PstNI CAGNNNCTG 2 cut(s) 335, 925
PsuI RGATCY 2 cut(s) 61, 598
PvuII CAGCTG 1 cut(s) 85
RsaI GTAC 4 cut(s) 112, 272, 827, 1115
RsaNI GTAC 4 cut(s) 111, 271, 826, 1114
RseI CAYNNNNRTG 2 cut(s) 402, 648
SacII CCGCGG 1 cut(s) 1120
SapI GCTCTTC 2 cut(s) 5, 62
SaqAI TTAA 2 cut(s) 294, 654
SatI GCNGC 9 cut(s) 22, 83, 86, 89, 92, 159, 357, 1007, 1088
Sau3AI GATC 8 cut(s) 52, 61, 202, 326, 434, 598, 880, 1071
Sau96I GGNCC 2 cut(s) 1081, 1121
ScrFI CCNGG 3 cut(s) 519, 1077, 1078
SduI GDGCHC 3 cut(s) 399, 529, 961
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 2 cut(s) 86, 231
Sfr303I CCGCGG 1 cut(s) 1120
SfuI TTCGAA 1 cut(s) 629
SgrBI CCGCGG 1 cut(s) 1120
SinI GGWCC 2 cut(s) 1081, 1121
SmaI CCCGGG 1 cut(s) 1078
SmiMI CAYNNNNRTG 2 cut(s) 402, 648
SmlI CTYRAG 3 cut(s) 366, 392, 1109
SmoI CTYRAG 3 cut(s) 366, 392, 1109
SpeI ACTAGT 1 cut(s) 577
SphI GCATGC 1 cut(s) 481
SspI AATATT 1 cut(s) 512
SspMI CTAG 3 cut(s) 302, 578, 993
StyD4I CCNGG 3 cut(s) 517, 1075, 1076
TaaI ACNGT 4 cut(s) 263, 691, 830, 860
TaiI ACGT 1 cut(s) 288
TaqI TCGA 2 cut(s) 164, 629
TatI WGTACW 1 cut(s) 825
TauI GCSGC 5 cut(s) 24, 94, 161, 1009, 1090
TfiI GAWTC 2 cut(s) 837, 922
Tru1I TTAA 2 cut(s) 294, 654
Tru9I TTAA 2 cut(s) 294, 654
TscAI CASTG 2 cut(s) 159, 534
TseFI GTSAC 2 cut(s) 863, 898
TseI GCWGC 4 cut(s) 82, 85, 88, 356
Tsp45I GTSAC 2 cut(s) 863, 898
TspDTI ATGAA 4 cut(s) 17, 614, 756, 989
TspMI CCCGGG 1 cut(s) 1076
TspRI CASTG 2 cut(s) 159, 534
VneI GTGCAC 1 cut(s) 525
VpaK11BI GGWCC 2 cut(s) 1081, 1121
XbaI TCTAGA 1 cut(s) 301
XceI RCATGY 5 cut(s) 111, 271, 481, 670, 871
XmaI CCCGGG 1 cut(s) 1076
XspI CTAG 3 cut(s) 302, 578, 993
ZraI GACGTC 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.