Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0010588

Physical Location & Seq

Genomic Coordinates Reverse (-)

3741354 .. 3745516

Gene Structure 4163 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1056600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1587 bp

ATGGCCGCCAAAACCCAAGACTCTGACAGAGTCACAGTTTCAGCACCGCCGCTGCTGCCGACGCAAAGACCGGTGATAACACTCCCCCCGCGGCCGTCCGCAGAGGCCTTTTTCAGCGGCGGATCTGGGGCGAGCCCGGGCCCGATGACTCTGGTCTCGAACTTCTTCGACACCTACCCTGAGTCGGATTTCAGGTCCTTCTCTCAGCTCCTAGCCGGAGCTATGGGCTCGCCGGTGGGTTCCACCAGGGGGATGAGCTTTTTTACCGAGAACCCAGCTGATGGTTTGAGAAAATCAGGCGGAGGTTCCGAAAGCGGGGAGGGTAGTTCCGGGTTTAAGCAGAGCAGGCCGATGAGTTTGGCGGTGGCTCGGCCGCCATTGTTTACTGTTCCTCCGGGGCTCAGCCCCTCTGGGTTGCTCAACTCGCCTGGCTTTTATTCACCTCAGAGTCCATTTGGAATGTCACACCAGCAGGCCTTGGCACAGGTTACTGCTCAAGCTGCACTAGCCCAATCTCGTGTGTACATGCAACCTGAGTATCAACCTTCTTCAGTAGCACCCCCAACAGAGCCAGAGGAACGTCAACTATCCTTAATGCCCAATGAAGCTTCACAGCAGCAGACATTGCCCTCAACATCTAATACTAAAAGTTCTGCAAGGCAATCCCCAGAGGCATCTCATTCTGATAAGAAATACCAACCTTCATCCACTGATAGACCTGCAGACGATAGCTACAACTGGCGGAAATATGGGCAGAAGCAGGTTAAAGGCAGTGAATTTCCTCGAAGCTACTACAAATGCACACATATGAATTGCCCTGTCAAAAAGAAAGTCGAGCGTTCTCCTAATGGCGAAATAACTGAGATTATCTACAAGGGGCAGCATAACCATGAAGTCCCTCAACCGAGTAAACGCCCAAAAGATGGTGATCTGAATGGGCCTAAGCCTGAAAACGGATTACAAAGAAGGATAGGAGATTCAAATAGGTCAAGTGAAAATGTAGCTTCGTACTCACGACGTGAGATGGATCAAGAATCTACTCAAGCCGCTCCTGGACAGTTACCGGGGGAAAATGACAATGAAGAGTTGGGCGATGGGGAAAGTAGAGAAGAGGGAGATGCTGATGAACCTAATGCAAAGAGAAGAAACATAGACGTTGGCGCATCTGAGGTAGCTTTGCCACATAAGACGGTCACAGAACCAAAAATCATTGTGCAAACAAGGAGTGAAGTTGATCTTTTAGATGATGGTTATAGGTGGCGCAAGTACGGGCAAAAGGTGGTCAAAGGGAATCCTCATCCGAGAAGCTATTACAAATGCACTTTTGCGGGATGCAATGTCCGTAAGCATGTTGAGAGAGCTTCCACTGACGCCAAAGCCGTCATCACTACATACGAGGGGAAACACAACCATGATGTTCCAGCAGCAAGAAACAGCAGCCACAACACGGCCAATAATAGTGCATCACAGTTAAAACCACCAGCGTCTGGGGCTTCACAGCATACCCTACTCAAAGGGAGAGAATATGGAAACAATGACCAGAGACCGGTACTTCTTCAGCTAAAAGAAGAACAAATCTTTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

529

Amino Acids

57.61

Weight (kDa)

8.62

Isoelectric Point (pI)

69.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	242 - 298	7e-27	WRKY DNA -binding domain
WRKY	PF03106	415 - 472	4.8e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010588)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g47800 FvH4_6g47800 FvH4_6g47800
malus_domestica	MD09G1056600.v1.1 MD17G1054100.v1.1
prunus_persica	Prupe.3G262100_v2.0.a1
pyrus_communis	pycom111g04740
rosa_chinensis	RchiOBHm_Chr2g0166991
rosa_laevigata	RLG00000021687
rosa_multiflora	Rmu_sc0010966.1_g000003 Rmu_sc0010966.1_g000004 Rmu_sc0025421.1_g000004
rosa_roxburghii	Rroxscaffold_2G00083830
rosa_rugosa	Rorug02G0527200 Rorug02G0527300 Rorug02G0527300
rosa_samantha	Rh2AG594100 Rh2BG605600 Rh2CG576500 Rh2DG617100
rosa_wichuraiana	Rw2G049470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	727, 751
AccB7I	CCANNNNNTGG	3 cut(s)	281, 923, 1487
AccBSI	CCGCTC	1 cut(s)	1049
AccII	CGCG	1 cut(s)	91
AclWI	GGATC	2 cut(s)	130, 1035
AcoI	YGGCCR	4 cut(s)	3, 92, 371, 1449
AcsI	RAATTY	1 cut(s)	776
AcuI	CTGAAG	2 cut(s)	534, 1541
AcyI	GRCGYC	1 cut(s)	1371
AdeI	CACNNNGTG	1 cut(s)	1019
AfaI	GTAC	4 cut(s)	524, 1010, 1268, 1551
AfiI	CCNNNNNNNGG	9 cut(s)	184, 249, 281, 315, 923, 953, 1447, 1487, 1546
AgeI	ACCGGT	2 cut(s)	70, 1546
AgsI	TTSAA	1 cut(s)	981
AjiI	CACGTC	1 cut(s)	1019
AjnI	CCWGG	3 cut(s)	245, 427, 1051
Alw26I	GTCTC	2 cut(s)	160, 1537
AlwI	GGATC	2 cut(s)	130, 1035
AlwNI	CAGNNNCTG	1 cut(s)	1487
Ama87I	CYCGRG	1 cut(s)	136
AoxI	GGCC	9 cut(s)	3, 92, 105, 139, 347, 371, 474, 938, 1449
ApaI	GGGCCC	1 cut(s)	143
ApeKI	GCWGC	7 cut(s)	52, 55, 500, 616, 880, 1424, 1437
ApoI	RAATTY	1 cut(s)	776
ArsI	GACNNNNNNTTYG	2 cut(s)	816, 848
AsiGI	ACCGGT	2 cut(s)	70, 1546
Asp700I	GAANNNNTTC	1 cut(s)	164
AspLEI	GCGC	2 cut(s)	1163, 1263
AspS9I	GGNCC	4 cut(s)	139, 140, 195, 938
AsuC2I	CCSGG	5 cut(s)	137, 138, 331, 396, 1065
AsuHPI	GGTGA	3 cut(s)	85, 432, 938
AvaI	CYCGRG	1 cut(s)	136
AvaII	GGWCC	1 cut(s)	195
BaeGI	GKGCMC	1 cut(s)	143
BaeI	ACNNNNGTAYC	2 cut(s)	521, 554
BanII	GRGCYC	4 cut(s)	137, 143, 230, 402
BauI	CACGAG	1 cut(s)	516
BbvI	GCAGC	7 cut(s)	39, 42, 487, 628, 892, 1436, 1449
BccI	CCATC	5 cut(s)	275, 917, 1018, 1088, 1241
BceAI	ACGGC	3 cut(s)	79, 1364, 1464
BciT130I	CCWGG	3 cut(s)	247, 429, 1053
BcnI	CCSGG	5 cut(s)	137, 138, 331, 396, 1065
BcoDI	GTCTC	2 cut(s)	160, 1537
BfaI	CTAG	2 cut(s)	212, 506
BfmI	CTRYAG	1 cut(s)	720
BfuAI	ACCTGC	2 cut(s)	727, 751
BlpI	GCTNAGC	1 cut(s)	401
Bme1390I	CCNGG	8 cut(s)	137, 138, 247, 331, 396, 429, 1053, 1065
Bme18I	GGWCC	1 cut(s)	195
BmeT110I	CYCGRG	1 cut(s)	136
BmgBI	CACGTC	1 cut(s)	1019
BmgT120I	GGNCC	4 cut(s)	139, 140, 195, 938
BmiI	GGNNCC	3 cut(s)	141, 241, 307
BmrFI	CCNGG	8 cut(s)	137, 138, 247, 331, 396, 429, 1053, 1065
BmsI	GCATC	5 cut(s)	683, 1108, 1172, 1322, 1472
BoxI	GACNNNNGTC	1 cut(s)	152
Bpu10I	CCTNAGC	1 cut(s)	942
Bpu1102I	GCTNAGC	1 cut(s)	401
BpuEI	CTTGAG	2 cut(s)	480, 1026
BpuMI	CCSGG	5 cut(s)	137, 138, 331, 396, 1065
BsaBI	GATNNNNATC	1 cut(s)	927
BsaHI	GRCGYC	1 cut(s)	1371
BsaI	GGTCTC	2 cut(s)	160, 1537
BsaJI	CCNNGG	6 cut(s)	89, 136, 246, 395, 477, 1064
BsaWI	WCCGGW	2 cut(s)	70, 1546
BsaXI	ACNNNNNCTCC	2 cut(s)	376, 406
Bsc4I	CCNNNNNNNGG	9 cut(s)	184, 249, 281, 315, 923, 953, 1447, 1487, 1546
Bse118I	RCCGGY	3 cut(s)	70, 232, 1546
Bse1I	ACTGG	1 cut(s)	743
Bse3DI	GCAATG	2 cut(s)	623, 1342
Bse8I	GATNNNNATC	1 cut(s)	927
BseBI	CCWGG	3 cut(s)	247, 429, 1053
BseDI	CCNNGG	6 cut(s)	89, 136, 246, 395, 477, 1064
BseGI	GGATG	4 cut(s)	258, 704, 1297, 1337
BseJI	GATNNNNATC	1 cut(s)	927
BseLI	CCNNNNNNNGG	9 cut(s)	184, 249, 281, 315, 923, 953, 1447, 1487, 1546
BseMI	GCAATG	2 cut(s)	623, 1342
BseMII	CTCAG	7 cut(s)	171, 218, 415, 458, 525, 852, 1158
BseNI	ACTGG	1 cut(s)	743
BseSI	GKGCMC	1 cut(s)	143
BseX3I	CGGCCG	2 cut(s)	92, 371
BseXI	GCAGC	7 cut(s)	39, 42, 487, 628, 892, 1436, 1449
BseYI	CCCAGC	1 cut(s)	274
BsgI	GTGCAG	1 cut(s)	486
Bsh1236I	CGCG	1 cut(s)	91
Bsh1285I	CGRYCG	2 cut(s)	95, 374
BshFI	GGCC	9 cut(s)	5, 94, 107, 141, 349, 373, 476, 940, 1451
BshTI	ACCGGT	2 cut(s)	70, 1546
BsiEI	CGRYCG	2 cut(s)	95, 374
BsiHKCI	CYCGRG	1 cut(s)	136
BsiSI	CCGG	8 cut(s)	71, 137, 216, 233, 330, 395, 1064, 1547
BslFI	GGGAC	1 cut(s)	881
BslI	CCNNNNNNNGG	9 cut(s)	184, 249, 281, 315, 923, 953, 1447, 1487, 1546
BsmAI	GTCTC	2 cut(s)	160, 1537
BsmFI	GGGAC	1 cut(s)	881
BsnI	GGCC	9 cut(s)	5, 94, 107, 141, 349, 373, 476, 940, 1451
Bso31I	GGTCTC	2 cut(s)	160, 1537
BsoBI	CYCGRG	1 cut(s)	136
Bsp120I	GGGCCC	1 cut(s)	139
Bsp1286I	GDGCHC	4 cut(s)	137, 143, 230, 402
Bsp1407I	TGTACA	1 cut(s)	522
Bsp143I	GATC	4 cut(s)	122, 928, 1027, 1234
Bsp1720I	GCTNAGC	1 cut(s)	401
BspANI	GGCC	9 cut(s)	5, 94, 107, 141, 349, 373, 476, 940, 1451
BspCNI	CTCAG	7 cut(s)	172, 217, 414, 457, 526, 853, 1159
BspFNI	CGCG	1 cut(s)	91
BspLI	GGNNCC	3 cut(s)	141, 241, 307
BspMAI	CTGCAG	1 cut(s)	724
BspMI	ACCTGC	2 cut(s)	727, 751
BspPI	GGATC	2 cut(s)	130, 1035
BspTNI	GGTCTC	2 cut(s)	160, 1537
BsrBI	CCGCTC	1 cut(s)	1049
BsrDI	GCAATG	2 cut(s)	623, 1342
BsrFI	RCCGGY	3 cut(s)	70, 232, 1546
BsrGI	TGTACA	1 cut(s)	522
BsrI	ACTGG	1 cut(s)	743
BssAI	RCCGGY	3 cut(s)	70, 232, 1546
BssECI	CCNNGG	6 cut(s)	89, 136, 246, 395, 477, 1064
BssMI	GATC	4 cut(s)	122, 928, 1027, 1234
BssNI	GRCGYC	1 cut(s)	1371
BssSI	CACGAG	1 cut(s)	516
BssT1I	CCWWGG	1 cut(s)	477
Bst2BI	CACGAG	1 cut(s)	516
Bst2UI	CCWGG	3 cut(s)	247, 429, 1053
Bst4CI	ACNGT	5 cut(s)	37, 388, 1059, 1192, 1470
Bst6I	CTCTTC	2 cut(s)	1077, 1104
BstACI	GRCGYC	1 cut(s)	1371
BstAPI	GCANNNNNTGC	1 cut(s)	625
BstAUI	TGTACA	1 cut(s)	522
BstC8I	GCNNGC	4 cut(s)	133, 230, 347, 474
BstDEI	CTNAG	8 cut(s)	180, 204, 401, 444, 534, 861, 942, 1167
BstDSI	CCRYGG	1 cut(s)	89
BstF5I	GGATG	4 cut(s)	258, 704, 1297, 1337
BstFNI	CGCG	1 cut(s)	91
BstHHI	GCGC	2 cut(s)	1163, 1263
BstKTI	GATC	4 cut(s)	125, 931, 1030, 1237
BstMAI	GTCTC	2 cut(s)	160, 1537
BstMBI	GATC	4 cut(s)	122, 928, 1027, 1234
BstMCI	CGRYCG	2 cut(s)	95, 374
BstMWI	GCNNNNNNNGC	8 cut(s)	55, 61, 346, 424, 500, 506, 625, 1490
BstNI	CCWGG	3 cut(s)	247, 429, 1053
BstNSI	RCATGY	2 cut(s)	529, 1352
BstPAI	GACNNNNGTC	1 cut(s)	152
BstSCI	CCNGG	8 cut(s)	135, 136, 245, 329, 394, 427, 1051, 1063
BstSFI	CTRYAG	1 cut(s)	720
BstSLI	GKGCMC	1 cut(s)	143
BstUI	CGCG	1 cut(s)	91
BstV1I	GCAGC	7 cut(s)	39, 42, 487, 628, 892, 1436, 1449
BstX2I	RGATCY	1 cut(s)	122
BstYI	RGATCY	1 cut(s)	122
BstZI	CGGCCG	2 cut(s)	92, 371
BsuRI	GGCC	9 cut(s)	5, 94, 107, 141, 349, 373, 476, 940, 1451
BtgI	CCRYGG	1 cut(s)	89
BtgZI	GCGATG	1 cut(s)	1107
BtrI	CACGTC	1 cut(s)	1019
BtsCI	GGATG	4 cut(s)	258, 704, 1297, 1337
BtsI	GCAGTG	1 cut(s)	778
BtsIMutI	CAGTG	3 cut(s)	708, 778, 1365
BveI	ACCTGC	2 cut(s)	727, 751
Cac8I	GCNNGC	4 cut(s)	133, 230, 347, 474
CaiI	CAGNNNCTG	1 cut(s)	1487
CfoI	GCGC	2 cut(s)	1163, 1263
Cfr10I	RCCGGY	3 cut(s)	70, 232, 1546
Cfr13I	GGNCC	4 cut(s)	139, 140, 195, 938
Cfr42I	CCGCGG	1 cut(s)	92
Cfr9I	CCCGGG	1 cut(s)	136
CseI	GACGC	3 cut(s)	70, 1379, 1473
Csp6I	GTAC	4 cut(s)	523, 1009, 1267, 1550
CspAI	ACCGGT	2 cut(s)	70, 1546
CviAII	CATG	4 cut(s)	526, 890, 1349, 1412
CviQI	GTAC	4 cut(s)	523, 1009, 1267, 1550
DdeI	CTNAG	8 cut(s)	180, 204, 401, 444, 534, 861, 942, 1167
DpnI	GATC	4 cut(s)	124, 930, 1029, 1236
DpnII	GATC	4 cut(s)	122, 928, 1027, 1234
DraIII	CACNNNGTG	1 cut(s)	1019
EaeI	YGGCCR	4 cut(s)	3, 92, 371, 1449
EagI	CGGCCG	2 cut(s)	92, 371
Eam1104I	CTCTTC	2 cut(s)	1077, 1104
EarI	CTCTTC	2 cut(s)	1077, 1104
EciI	GGCGGA	3 cut(s)	135, 315, 757
EclXI	CGGCCG	2 cut(s)	92, 371
Eco130I	CCWWGG	1 cut(s)	477
Eco147I	AGGCCT	2 cut(s)	107, 476
Eco24I	GRGCYC	4 cut(s)	137, 143, 230, 402
Eco31I	GGTCTC	2 cut(s)	160, 1537
Eco47I	GGWCC	1 cut(s)	195
Eco52I	CGGCCG	2 cut(s)	92, 371
Eco57I	CTGAAG	2 cut(s)	534, 1541
Eco88I	CYCGRG	1 cut(s)	136
EcoO109I	RGGNCCY	1 cut(s)	195
EcoRII	CCWGG	3 cut(s)	245, 427, 1051
EcoT14I	CCWWGG	1 cut(s)	477
EcoT38I	GRGCYC	4 cut(s)	137, 143, 230, 402
ErhI	CCWWGG	1 cut(s)	477
FaeI	CATG	4 cut(s)	529, 893, 1352, 1415
FalI	AAGNNNNNCTT	2 cut(s)	1221, 1253
FaqI	GGGAC	1 cut(s)	881
FatI	CATG	4 cut(s)	525, 889, 1348, 1411
FauI	CCCGC	3 cut(s)	96, 308, 1321
FauNDI	CATATG	1 cut(s)	807
FokI	GGATG	4 cut(s)	265, 691, 1284, 1344
FriOI	GRGCYC	4 cut(s)	137, 143, 230, 402
FspBI	CTAG	2 cut(s)	212, 506
GlaI	GCGC	2 cut(s)	1162, 1262
GsaI	CCCAGC	1 cut(s)	278
HaeIII	GGCC	9 cut(s)	5, 94, 107, 141, 349, 373, 476, 940, 1451
HapII	CCGG	8 cut(s)	71, 137, 216, 233, 330, 395, 1064, 1547
HgaI	GACGC	3 cut(s)	70, 1379, 1473
HhaI	GCGC	2 cut(s)	1163, 1263
Hin1I	GRCGYC	1 cut(s)	1371
Hin1II	CATG	4 cut(s)	529, 893, 1352, 1415
Hin6I	GCGC	2 cut(s)	1161, 1261
HinP1I	GCGC	2 cut(s)	1161, 1261
HincII	GTYRAC	1 cut(s)	584
HindII	GTYRAC	1 cut(s)	584
HindIII	AAGCTT	1 cut(s)	606
HinfI	GANTC	8 cut(s)	20, 30, 148, 182, 448, 977, 1034, 1291
HpaII	CCGG	8 cut(s)	71, 137, 216, 233, 330, 395, 1064, 1547
HphI	GGTGA	3 cut(s)	85, 432, 938
Hpy166II	GTNNAC	4 cut(s)	384, 523, 584, 911
Hpy188I	TCNGA	8 cut(s)	25, 187, 310, 447, 685, 933, 1168, 1302
Hpy188III	TCNNGA	3 cut(s)	157, 1014, 1031
Hpy8I	GTNNAC	4 cut(s)	384, 523, 584, 911
Hpy99I	CGWCG	2 cut(s)	64, 1020
HpyAV	CCTTC	4 cut(s)	208, 555, 711, 960
HpyCH4III	ACNGT	5 cut(s)	37, 388, 1059, 1192, 1470
HpyCH4IV	ACGT	3 cut(s)	580, 1018, 1155
HpyF10VI	GCNNNNNNNGC	8 cut(s)	55, 61, 346, 424, 500, 506, 625, 1490
HpyF3I	CTNAG	8 cut(s)	180, 204, 401, 444, 534, 861, 942, 1167
HpySE526I	ACGT	3 cut(s)	580, 1018, 1155
Hsp92I	GRCGYC	1 cut(s)	1371
Hsp92II	CATG	4 cut(s)	529, 893, 1352, 1415
HspAI	GCGC	2 cut(s)	1161, 1261
KspI	CCGCGG	1 cut(s)	92
Kzo9I	GATC	4 cut(s)	122, 928, 1027, 1234
LmnI	GCTCC	3 cut(s)	213, 218, 1054
Lsp1109I	GCAGC	7 cut(s)	39, 42, 487, 628, 892, 1436, 1449
LweI	GCATC	5 cut(s)	683, 1108, 1172, 1322, 1472
MaeI	CTAG	2 cut(s)	212, 506
MaeII	ACGT	3 cut(s)	580, 1018, 1155
MaeIII	GTNAC	5 cut(s)	31, 462, 487, 1059, 1192
MalI	GATC	4 cut(s)	124, 930, 1029, 1236
MbiI	CCGCTC	1 cut(s)	1049
MboI	GATC	4 cut(s)	122, 928, 1027, 1234
MboII	GAAGA	7 cut(s)	157, 540, 1094, 1121, 1155, 1547, 1580
MflI	RGATCY	1 cut(s)	122
MhlI	GDGCHC	4 cut(s)	137, 143, 230, 402
MluCI	AATT	2 cut(s)	776, 811
MlyI	GAGTC	5 cut(s)	14, 39, 142, 191, 457
MmeI	TCCRAC	1 cut(s)	165
MroXI	GAANNNNTTC	1 cut(s)	164
MseI	TTAA	5 cut(s)	336, 593, 765, 1472, 1585
MslI	CAYNNNNRTG	3 cut(s)	806, 888, 1410
MspA1I	CMGCKG	4 cut(s)	52, 91, 117, 278
MspI	CCGG	8 cut(s)	71, 137, 216, 233, 330, 395, 1064, 1547
MspR9I	CCNGG	8 cut(s)	137, 138, 247, 331, 396, 429, 1053, 1065
MvaI	CCWGG	3 cut(s)	247, 429, 1053
MvnI	CGCG	1 cut(s)	91
MwoI	GCNNNNNNNGC	8 cut(s)	55, 61, 346, 424, 500, 506, 625, 1490
NciI	CCSGG	5 cut(s)	137, 138, 331, 396, 1065
NdeI	CATATG	1 cut(s)	807
NdeII	GATC	4 cut(s)	122, 928, 1027, 1234
NlaIII	CATG	4 cut(s)	529, 893, 1352, 1415
NlaIV	GGNNCC	3 cut(s)	141, 241, 307
NmeAIII	GCCGAG	1 cut(s)	349
NmuCI	GTSAC	3 cut(s)	31, 462, 1192
NspI	RCATGY	2 cut(s)	529, 1352
PceI	AGGCCT	2 cut(s)	107, 476
PcsI	WCGNNNNNNNCGW	1 cut(s)	1377
PdmI	GAANNNNTTC	1 cut(s)	164
PfeI	GAWTC	3 cut(s)	977, 1034, 1291
PflFI	GACNNNGTC	1 cut(s)	29
PflMI	CCANNNNNTGG	3 cut(s)	281, 923, 1487
PfoI	TCCNGGA	1 cut(s)	1051
PinAI	ACCGGT	2 cut(s)	70, 1546
PleI	GAGTC	5 cut(s)	14, 38, 142, 190, 456
PpsI	GAGTC	5 cut(s)	14, 38, 142, 190, 456
PpuMI	RGGWCCY	1 cut(s)	195
PshAI	GACNNNNGTC	1 cut(s)	152
Psp5II	RGGWCCY	1 cut(s)	195
Psp6I	CCWGG	3 cut(s)	245, 427, 1051
PspFI	CCCAGC	1 cut(s)	274
PspGI	CCWGG	3 cut(s)	245, 427, 1051
PspN4I	GGNNCC	3 cut(s)	141, 241, 307
PspOMI	GGGCCC	1 cut(s)	139
PspPI	GGNCC	4 cut(s)	139, 140, 195, 938
PspPPI	RGGWCCY	1 cut(s)	195
PstI	CTGCAG	1 cut(s)	724
PstNI	CAGNNNCTG	1 cut(s)	1487
PsuI	RGATCY	1 cut(s)	122
PsyI	GACNNNGTC	1 cut(s)	29
PvuII	CAGCTG	1 cut(s)	278
RsaI	GTAC	4 cut(s)	524, 1010, 1268, 1551
RsaNI	GTAC	4 cut(s)	523, 1009, 1267, 1550
RseI	CAYNNNNRTG	3 cut(s)	806, 888, 1410
SacII	CCGCGG	1 cut(s)	92
SaqAI	TTAA	5 cut(s)	336, 593, 765, 1472, 1585
Sau3AI	GATC	4 cut(s)	122, 928, 1027, 1234
Sau96I	GGNCC	4 cut(s)	139, 140, 195, 938
SchI	GAGTC	5 cut(s)	14, 39, 142, 191, 457
ScrFI	CCNGG	8 cut(s)	137, 138, 247, 331, 396, 429, 1053, 1065
SduI	GDGCHC	4 cut(s)	137, 143, 230, 402
SfaNI	GCATC	5 cut(s)	683, 1108, 1172, 1322, 1472
SfcI	CTRYAG	1 cut(s)	720
Sfr303I	CCGCGG	1 cut(s)	92
SgrAI	CRCCGGYG	1 cut(s)	232
SgrBI	CCGCGG	1 cut(s)	92
SinI	GGWCC	1 cut(s)	195
SmaI	CCCGGG	1 cut(s)	138
SmiMI	CAYNNNNRTG	3 cut(s)	806, 888, 1410
SmlI	CTYRAG	2 cut(s)	495, 1041
SmoI	CTYRAG	2 cut(s)	495, 1041
SrfI	GCCCGGGC	1 cut(s)	138
Sse9I	AATT	2 cut(s)	776, 811
SseBI	AGGCCT	2 cut(s)	107, 476
SspMI	CTAG	2 cut(s)	212, 506
StuI	AGGCCT	2 cut(s)	107, 476
StyD4I	CCNGG	8 cut(s)	135, 136, 245, 329, 394, 427, 1051, 1063
StyI	CCWWGG	1 cut(s)	477
TaaI	ACNGT	5 cut(s)	37, 388, 1059, 1192, 1470
TaiI	ACGT	3 cut(s)	583, 1021, 1158
TaqI	TCGA	4 cut(s)	158, 168, 784, 834
TasI	AATT	2 cut(s)	776, 811
TatI	WGTACW	1 cut(s)	522
TauI	GCSGC	6 cut(s)	8, 52, 94, 120, 376, 1049
TfiI	GAWTC	3 cut(s)	977, 1034, 1291
Tru1I	TTAA	5 cut(s)	336, 593, 765, 1472, 1585
Tru9I	TTAA	5 cut(s)	336, 593, 765, 1472, 1585
TscAI	CASTG	3 cut(s)	715, 778, 1372
TseFI	GTSAC	3 cut(s)	31, 462, 1192
TseI	GCWGC	7 cut(s)	52, 55, 500, 616, 880, 1424, 1437
Tsp45I	GTSAC	3 cut(s)	31, 462, 1192
TspDTI	ATGAA	6 cut(s)	618, 693, 824, 906, 1095, 1140
TspGWI	ACGGA	2 cut(s)	969, 1331
TspMI	CCCGGG	1 cut(s)	136
TspRI	CASTG	3 cut(s)	715, 778, 1372
Tth111I	GACNNNGTC	1 cut(s)	29
Van91I	CCANNNNNTGG	3 cut(s)	281, 923, 1487
VpaK11BI	GGWCC	1 cut(s)	195
XapI	RAATTY	1 cut(s)	776
XceI	RCATGY	2 cut(s)	529, 1352
XmaI	CCCGGG	1 cut(s)	136
XmnI	GAANNNNTTC	1 cut(s)	164
XspI	CTAG	2 cut(s)	212, 506

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.