Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0010588

Physical Location & Seq

Genomic Coordinates Reverse (-)

4198534 .. 4202033

Gene Structure 3500 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1054100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

ATGACCGCCAAAACCCAAGACTCGAACAGGGTCGCAGTTTCAGCTCCGCCGCCACAGAAGCAGCCACAAAGACCAGTGATAACACTCCCGCCGCGGCCGTCCGCGGAGGCCCTTTTCAGCGGCGGATCTGGGGCGAGCCCGGGTCCCATGACTCTTGTCTCGAGCTTCTTCGAGAACTACCCTGAGTCGGATTTCAGGTCCTTCTCTCAGCTCCTCGCCGGAGCCATGGGCTCGCCGGTGGGGTCCACAAGGGGGATGAACTTTTTTAGCGATAGCCCAGCTGATGGTTTGGGAAAATCAGGCGGAGGTTCCGAATATGGCGGGGAGGGCAGTTCTGGGTTTAAGCAGAGTAGGCCGATGAGTTTGGCGGTGGCTCGGCCGCCATTGTTTACTGTCCCGCCGGGACTCAGCCCCTCTGGGTTGCTTAACTCGCCTGGCTTCTATTCACCTCAGAGTCCGTTTGGAATGTCACACCAGCAGGCCTTGGCACAGGTTACTGCTCAAGCTGCATTAGCCCAATCTCGTGTGTACATGCAACCTGATTACCAACCTTCTTCAGTAGCAGCCCCGACAGAGCCACAGGCATGTCAACCATCTTTAATGCCCGAGGAAGCTTCTCGGCAGCTTACATTGCCCTCAACATCTGATACTAAAAGTTCTGTGAGGCAATCCTCAGAGGCATCTCATTCTGATGGGAAATACCAACCTTCATCCACTGGTAGGCCTGCGGACGATAGCTACAACTGGCGGAAATATGGGCAGAAGCTGGTTAAGGGCAGTGAATTTCCTCGAAGCTACTACAAGTGCACACATATGAATTGCCCTGTCAAAAAGAAAGTCGAGCGTATTCCTAGTGGCGAAATAACTGAGATTATCTACAAAGGTCAGCATAACCATGAAGTCCCTCAACCGAGTAAACGCCCAAAAGATGGTGATCTGAATGGGCCTAAGCCTGAAAACGGATTACAAAGAAGGGTAGGAGATTCAAATAGGTTGAGTGAAAATATAGCTTCTCACTCACAACCTGAAAGGGATCAGGAATCTACTGAAGCCGCTTCTGGACAGTTACCAGGGGGAATTGACAATGAAGAGTTGGGCGATGGGGAAACTAGAGAAGAGGGAGATGCTGATGAACCAAATGCAAAGAGAAGGAACATAGACGTTGGCGCATCTGAGGTAGCTTTGGCACACAAGATGGTCACAGAACCAAAAATCATTGTGCAGACAAGGAGTGAAGTTGATCTTCTCGATGATGGCTATAGGTGGCGCAAGTACGGGCAAAAGGTGGTCAAAGGGAACCCTCATCCAAGGAGCTATTACAAATGCACGTTTGCGGGGTGCAATGTCCGTAAGCATGTTGAGAGAGCTTCCACTGACGCTGAAGCTGTCATCACTACATATGAAGGCAAACACAACCATGATGTTCCAGCAGCAAGAAACAGCAGCCACAGCACGGCCAATAATAGTGCATCACTGTTAAAACCACCCGCGTCCAGGGCTCCTCAGCATACCCTACAGAAGGGCAGAGAATATGGAAACAATGATCAGAGACCGGTACTTCTTCAGCTAAAAGAAGAACAAATAGTTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

531

Amino Acids

57.38

Weight (kDa)

8.39

Isoelectric Point (pI)

68.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	244 - 300	5e-26	WRKY DNA -binding domain
WRKY	PF03106	417 - 474	3.4e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010588)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g47800 FvH4_6g47800 FvH4_6g47800
malus_domestica	MD09G1056600.v1.1 MD17G1054100.v1.1
prunus_persica	Prupe.3G262100_v2.0.a1
pyrus_communis	pycom111g04740
rosa_chinensis	RchiOBHm_Chr2g0166991
rosa_laevigata	RLG00000021687
rosa_multiflora	Rmu_sc0010966.1_g000003 Rmu_sc0010966.1_g000004 Rmu_sc0025421.1_g000004
rosa_roxburghii	Rroxscaffold_2G00083830
rosa_rugosa	Rorug02G0527200 Rorug02G0527300 Rorug02G0527300
rosa_samantha	Rh2AG594100 Rh2BG605600 Rh2CG576500 Rh2DG617100
rosa_wichuraiana	Rw2G049470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	284, 929
AccII	CGCG	3 cut(s)	94, 104, 1490
AclWI	GGATC	2 cut(s)	133, 1041
AcoI	YGGCCR	3 cut(s)	95, 377, 1455
AcsI	RAATTY	1 cut(s)	782
AcuI	CTGAAG	4 cut(s)	540, 1068, 1401, 1547
AfaI	GTAC	3 cut(s)	530, 1274, 1557
AfiI	CCNNNNNNNGG	9 cut(s)	187, 252, 284, 720, 929, 959, 1453, 1494, 1519
AgeI	ACCGGT	1 cut(s)	1552
AgsI	TTSAA	1 cut(s)	987
AjnI	CCWGG	3 cut(s)	433, 1069, 1493
Alw21I	GWGCWC	1 cut(s)	809
Alw26I	GTCTC	2 cut(s)	163, 1543
Alw44I	GTGCAC	1 cut(s)	805
AlwI	GGATC	2 cut(s)	133, 1041
AlwNI	CAGNNNCTG	1 cut(s)	766
Ama87I	CYCGRG	3 cut(s)	139, 160, 605
AoxI	GGCC	8 cut(s)	95, 108, 353, 377, 480, 722, 944, 1455
ApaLI	GTGCAC	1 cut(s)	805
ApeKI	GCWGC	6 cut(s)	61, 506, 563, 622, 1430, 1443
ApoI	RAATTY	1 cut(s)	782
ArsI	GACNNNNNNTTYG	2 cut(s)	822, 854
AsiGI	ACCGGT	1 cut(s)	1552
AspLEI	GCGC	2 cut(s)	1169, 1269
AspS9I	GGNCC	5 cut(s)	109, 143, 198, 243, 944
AsuC2I	CCSGG	3 cut(s)	140, 141, 402
AsuHPI	GGTGA	2 cut(s)	438, 944
AvaI	CYCGRG	3 cut(s)	139, 160, 605
AvaII	GGWCC	3 cut(s)	143, 198, 243
BaeGI	GKGCMC	1 cut(s)	809
BanII	GRGCYC	3 cut(s)	140, 233, 1501
BauI	CACGAG	1 cut(s)	522
Bbv12I	GWGCWC	1 cut(s)	809
BbvCI	CCTCAGC	1 cut(s)	1503
BbvI	GCAGC	6 cut(s)	73, 493, 575, 634, 1442, 1455
BccI	CCATC	7 cut(s)	278, 601, 686, 923, 1094, 1189, 1247
BceAI	ACGGC	2 cut(s)	82, 1470
BciT130I	CCWGG	3 cut(s)	435, 1071, 1495
BclI	TGATCA	1 cut(s)	1543
BcnI	CCSGG	3 cut(s)	140, 141, 402
BcoDI	GTCTC	2 cut(s)	163, 1543
BfaI	CTAG	2 cut(s)	852, 1110
BfmI	CTRYAG	2 cut(s)	1258, 1514
Bme1390I	CCNGG	6 cut(s)	140, 141, 402, 435, 1071, 1495
Bme18I	GGWCC	3 cut(s)	143, 198, 243
BmeT110I	CYCGRG	3 cut(s)	139, 160, 605
BmgT120I	GGNCC	5 cut(s)	109, 143, 198, 243, 944
BmiI	GGNNCC	7 cut(s)	144, 145, 223, 244, 310, 1298, 1500
BmrFI	CCNGG	6 cut(s)	140, 141, 402, 435, 1071, 1495
BmsI	GCATC	4 cut(s)	689, 1114, 1178, 1478
BoxI	GACNNNNGTC	1 cut(s)	155
Bpu10I	CCTNAGC	2 cut(s)	948, 1503
BpuEI	CTTGAG	1 cut(s)	486
BpuMI	CCSGG	3 cut(s)	140, 141, 402
BsaBI	GATNNNNATC	1 cut(s)	933
BsaI	GGTCTC	1 cut(s)	1543
BsaJI	CCNNGG	9 cut(s)	92, 102, 139, 225, 483, 606, 1070, 1307, 1494
BsaWI	WCCGGW	1 cut(s)	1552
Bsc4I	CCNNNNNNNGG	9 cut(s)	187, 252, 284, 720, 929, 959, 1453, 1494, 1519
Bse118I	RCCGGY	2 cut(s)	235, 1552
Bse1I	ACTGG	3 cut(s)	74, 721, 749
Bse3DI	GCAATG	2 cut(s)	629, 1348
Bse8I	GATNNNNATC	1 cut(s)	933
BseBI	CCWGG	3 cut(s)	435, 1071, 1495
BseDI	CCNNGG	9 cut(s)	92, 102, 139, 225, 483, 606, 1070, 1307, 1494
BseGI	GGATG	3 cut(s)	261, 710, 1303
BseJI	GATNNNNATC	1 cut(s)	933
BseLI	CCNNNNNNNGG	9 cut(s)	187, 252, 284, 720, 929, 959, 1453, 1494, 1519
BseMI	GCAATG	2 cut(s)	629, 1348
BseMII	CTCAG	8 cut(s)	174, 221, 421, 464, 687, 858, 1164, 1517
BseNI	ACTGG	3 cut(s)	74, 721, 749
BseRI	GAGGAG	2 cut(s)	203, 1491
BseSI	GKGCMC	1 cut(s)	809
BseX3I	CGGCCG	2 cut(s)	95, 377
BseXI	GCAGC	6 cut(s)	73, 493, 575, 634, 1442, 1455
BseYI	CCCAGC	1 cut(s)	277
BsgI	GTGCAG	1 cut(s)	1241
Bsh1236I	CGCG	3 cut(s)	94, 104, 1490
Bsh1285I	CGRYCG	2 cut(s)	98, 380
BshFI	GGCC	8 cut(s)	97, 110, 355, 379, 482, 724, 946, 1457
BshTI	ACCGGT	1 cut(s)	1552
BsiEI	CGRYCG	2 cut(s)	98, 380
BsiHKAI	GWGCWC	1 cut(s)	809
BsiHKCI	CYCGRG	3 cut(s)	139, 160, 605
BsiSI	CCGG	5 cut(s)	140, 219, 236, 401, 1553
BslFI	GGGAC	4 cut(s)	129, 380, 417, 887
BslI	CCNNNNNNNGG	9 cut(s)	187, 252, 284, 720, 929, 959, 1453, 1494, 1519
BsmAI	GTCTC	2 cut(s)	163, 1543
BsmFI	GGGAC	4 cut(s)	129, 380, 417, 887
BsnI	GGCC	8 cut(s)	97, 110, 355, 379, 482, 724, 946, 1457
Bso31I	GGTCTC	1 cut(s)	1543
BsoBI	CYCGRG	3 cut(s)	139, 160, 605
Bsp1286I	GDGCHC	4 cut(s)	140, 233, 809, 1501
Bsp1407I	TGTACA	1 cut(s)	528
Bsp143I	GATC	5 cut(s)	125, 934, 1033, 1240, 1543
Bsp19I	CCATGG	1 cut(s)	225
BspANI	GGCC	8 cut(s)	97, 110, 355, 379, 482, 724, 946, 1457
BspCNI	CTCAG	8 cut(s)	175, 220, 420, 463, 686, 859, 1165, 1516
BspFNI	CGCG	3 cut(s)	94, 104, 1490
BspLI	GGNNCC	7 cut(s)	144, 145, 223, 244, 310, 1298, 1500
BspPI	GGATC	2 cut(s)	133, 1041
BspTNI	GGTCTC	1 cut(s)	1543
BsrDI	GCAATG	2 cut(s)	629, 1348
BsrFI	RCCGGY	2 cut(s)	235, 1552
BsrGI	TGTACA	1 cut(s)	528
BsrI	ACTGG	3 cut(s)	74, 721, 749
BssAI	RCCGGY	2 cut(s)	235, 1552
BssECI	CCNNGG	9 cut(s)	92, 102, 139, 225, 483, 606, 1070, 1307, 1494
BssMI	GATC	5 cut(s)	125, 934, 1033, 1240, 1543
BssSI	CACGAG	1 cut(s)	522
BssT1I	CCWWGG	3 cut(s)	225, 483, 1307
Bst2BI	CACGAG	1 cut(s)	522
Bst2UI	CCWGG	3 cut(s)	435, 1071, 1495
Bst4CI	ACNGT	3 cut(s)	394, 1065, 1476
Bst6I	CTCTTC	2 cut(s)	1083, 1110
BstAUI	TGTACA	1 cut(s)	528
BstC8I	GCNNGC	4 cut(s)	136, 233, 480, 726
BstDEI	CTNAG	9 cut(s)	183, 207, 407, 450, 673, 867, 948, 1173, 1503
BstDSI	CCRYGG	3 cut(s)	92, 102, 225
BstENI	CCTNNNNNAGG	1 cut(s)	1517
BstF5I	GGATG	3 cut(s)	261, 710, 1303
BstFNI	CGCG	3 cut(s)	94, 104, 1490
BstHHI	GCGC	2 cut(s)	1169, 1269
BstKTI	GATC	5 cut(s)	128, 937, 1036, 1243, 1546
BstMAI	GTCTC	2 cut(s)	163, 1543
BstMBI	GATC	5 cut(s)	125, 934, 1033, 1240, 1543
BstMCI	CGRYCG	2 cut(s)	98, 380
BstNI	CCWGG	3 cut(s)	435, 1071, 1495
BstNSI	RCATGY	3 cut(s)	535, 588, 1358
BstPAI	GACNNNNGTC	1 cut(s)	155
BstSCI	CCNGG	6 cut(s)	138, 139, 400, 433, 1069, 1493
BstSFI	CTRYAG	2 cut(s)	1258, 1514
BstSLI	GKGCMC	1 cut(s)	809
BstUI	CGCG	3 cut(s)	94, 104, 1490
BstV1I	GCAGC	6 cut(s)	73, 493, 575, 634, 1442, 1455
BstX2I	RGATCY	1 cut(s)	125
BstYI	RGATCY	1 cut(s)	125
BstZI	CGGCCG	2 cut(s)	95, 377
BsuRI	GGCC	8 cut(s)	97, 110, 355, 379, 482, 724, 946, 1457
BtgI	CCRYGG	3 cut(s)	92, 102, 225
BtgZI	GCGATG	1 cut(s)	1113
BtsCI	GGATG	3 cut(s)	261, 710, 1303
BtsI	GCAGTG	1 cut(s)	784
BtsIMutI	CAGTG	5 cut(s)	81, 714, 784, 1371, 1472
Cac8I	GCNNGC	4 cut(s)	136, 233, 480, 726
CaiI	CAGNNNCTG	1 cut(s)	766
CfoI	GCGC	2 cut(s)	1169, 1269
Cfr10I	RCCGGY	2 cut(s)	235, 1552
Cfr13I	GGNCC	5 cut(s)	109, 143, 198, 243, 944
Cfr42I	CCGCGG	2 cut(s)	95, 105
Cfr9I	CCCGGG	1 cut(s)	139
CseI	GACGC	2 cut(s)	1385, 1479
Csp6I	GTAC	3 cut(s)	529, 1273, 1556
CspAI	ACCGGT	1 cut(s)	1552
CviAII	CATG	7 cut(s)	148, 226, 532, 585, 896, 1355, 1418
CviQI	GTAC	3 cut(s)	529, 1273, 1556
DdeI	CTNAG	9 cut(s)	183, 207, 407, 450, 673, 867, 948, 1173, 1503
DpnI	GATC	5 cut(s)	127, 936, 1035, 1242, 1545
DpnII	GATC	5 cut(s)	125, 934, 1033, 1240, 1543
EaeI	YGGCCR	3 cut(s)	95, 377, 1455
EagI	CGGCCG	2 cut(s)	95, 377
Eam1104I	CTCTTC	2 cut(s)	1083, 1110
EarI	CTCTTC	2 cut(s)	1083, 1110
EciI	GGCGGA	4 cut(s)	36, 138, 318, 763
EclXI	CGGCCG	2 cut(s)	95, 377
Eco130I	CCWWGG	3 cut(s)	225, 483, 1307
Eco147I	AGGCCT	2 cut(s)	482, 724
Eco24I	GRGCYC	3 cut(s)	140, 233, 1501
Eco31I	GGTCTC	1 cut(s)	1543
Eco47I	GGWCC	3 cut(s)	143, 198, 243
Eco52I	CGGCCG	2 cut(s)	95, 377
Eco57I	CTGAAG	4 cut(s)	540, 1068, 1401, 1547
Eco88I	CYCGRG	3 cut(s)	139, 160, 605
EcoNI	CCTNNNNNAGG	1 cut(s)	1517
EcoO109I	RGGNCCY	3 cut(s)	109, 143, 198
EcoRII	CCWGG	3 cut(s)	433, 1069, 1493
EcoT14I	CCWWGG	3 cut(s)	225, 483, 1307
EcoT38I	GRGCYC	3 cut(s)	140, 233, 1501
ErhI	CCWWGG	3 cut(s)	225, 483, 1307
FaeI	CATG	7 cut(s)	151, 229, 535, 588, 899, 1358, 1421
FalI	AAGNNNNNCTT	2 cut(s)	1227, 1259
FaqI	GGGAC	4 cut(s)	129, 380, 417, 887
FatI	CATG	7 cut(s)	147, 225, 531, 584, 895, 1354, 1417
FauI	CCCGC	5 cut(s)	96, 314, 405, 1327, 1495
FauNDI	CATATG	2 cut(s)	813, 1399
FbaI	TGATCA	1 cut(s)	1543
FokI	GGATG	3 cut(s)	268, 697, 1290
FriOI	GRGCYC	3 cut(s)	140, 233, 1501
FspBI	CTAG	2 cut(s)	852, 1110
GlaI	GCGC	2 cut(s)	1168, 1268
GsaI	CCCAGC	1 cut(s)	281
HaeIII	GGCC	8 cut(s)	97, 110, 355, 379, 482, 724, 946, 1457
HapII	CCGG	5 cut(s)	140, 219, 236, 401, 1553
HgaI	GACGC	2 cut(s)	1385, 1479
HhaI	GCGC	2 cut(s)	1169, 1269
Hin1II	CATG	7 cut(s)	151, 229, 535, 588, 899, 1358, 1421
Hin6I	GCGC	2 cut(s)	1167, 1267
HinP1I	GCGC	2 cut(s)	1167, 1267
HincII	GTYRAC	1 cut(s)	590
HindII	GTYRAC	1 cut(s)	590
HindIII	AAGCTT	1 cut(s)	612
HinfI	GANTC	7 cut(s)	20, 151, 185, 405, 454, 983, 1040
HpaII	CCGG	5 cut(s)	140, 219, 236, 401, 1553
HphI	GGTGA	2 cut(s)	438, 944
Hpy166II	GTNNAC	6 cut(s)	246, 390, 529, 590, 807, 917
Hpy188I	TCNGA	9 cut(s)	190, 313, 453, 646, 676, 691, 939, 1174, 1548
Hpy188III	TCNNGA	5 cut(s)	160, 172, 1037, 1059, 1247
Hpy8I	GTNNAC	6 cut(s)	246, 390, 529, 590, 807, 917
HpyAV	CCTTC	7 cut(s)	211, 561, 717, 966, 1143, 1397, 1513
HpyCH4III	ACNGT	3 cut(s)	394, 1065, 1476
HpyCH4IV	ACGT	2 cut(s)	1161, 1328
HpyCH4V	TGCA	8 cut(s)	509, 535, 807, 1142, 1222, 1326, 1341, 1469
HpyF3I	CTNAG	9 cut(s)	183, 207, 407, 450, 673, 867, 948, 1173, 1503
HpySE526I	ACGT	2 cut(s)	1161, 1328
Hsp92II	CATG	7 cut(s)	151, 229, 535, 588, 899, 1358, 1421
HspAI	GCGC	2 cut(s)	1167, 1267
KflI	GGGWCCC	1 cut(s)	143
Ksp22I	TGATCA	1 cut(s)	1543
KspI	CCGCGG	2 cut(s)	95, 105
Kzo9I	GATC	5 cut(s)	125, 934, 1033, 1240, 1543
LmnI	GCTCC	5 cut(s)	49, 216, 221, 1311, 1504
Lsp1109I	GCAGC	6 cut(s)	73, 493, 575, 634, 1442, 1455
LweI	GCATC	4 cut(s)	689, 1114, 1178, 1478
MaeI	CTAG	2 cut(s)	852, 1110
MaeII	ACGT	2 cut(s)	1161, 1328
MaeIII	GTNAC	4 cut(s)	468, 493, 1065, 1198
MalI	GATC	5 cut(s)	127, 936, 1035, 1242, 1545
MboI	GATC	5 cut(s)	125, 934, 1033, 1240, 1543
MboII	GAAGA	7 cut(s)	160, 546, 1100, 1127, 1235, 1553, 1586
MflI	RGATCY	1 cut(s)	125
MhlI	GDGCHC	4 cut(s)	140, 233, 809, 1501
MluCI	AATT	3 cut(s)	782, 817, 1077
MlyI	GAGTC	5 cut(s)	14, 145, 194, 399, 463
MmeI	TCCRAC	1 cut(s)	168
MseI	TTAA	6 cut(s)	342, 426, 599, 771, 1478, 1591
MslI	CAYNNNNRTG	5 cut(s)	583, 690, 812, 894, 1416
MspA1I	CMGCKG	4 cut(s)	94, 104, 120, 281
MspI	CCGG	5 cut(s)	140, 219, 236, 401, 1553
MspR9I	CCNGG	6 cut(s)	140, 141, 402, 435, 1071, 1495
MvaI	CCWGG	3 cut(s)	435, 1071, 1495
MvnI	CGCG	3 cut(s)	94, 104, 1490
NciI	CCSGG	3 cut(s)	140, 141, 402
NcoI	CCATGG	1 cut(s)	225
NdeI	CATATG	2 cut(s)	813, 1399
NdeII	GATC	5 cut(s)	125, 934, 1033, 1240, 1543
NlaIII	CATG	7 cut(s)	151, 229, 535, 588, 899, 1358, 1421
NlaIV	GGNNCC	7 cut(s)	144, 145, 223, 244, 310, 1298, 1500
NmeAIII	GCCGAG	2 cut(s)	355, 598
NmuCI	GTSAC	2 cut(s)	468, 1198
NspI	RCATGY	3 cut(s)	535, 588, 1358
PaeR7I	CTCGAG	1 cut(s)	160
PceI	AGGCCT	2 cut(s)	482, 724
PfeI	GAWTC	2 cut(s)	983, 1040
PflMI	CCANNNNNTGG	2 cut(s)	284, 929
PinAI	ACCGGT	1 cut(s)	1552
PleI	GAGTC	5 cut(s)	14, 145, 193, 399, 462
PpsI	GAGTC	5 cut(s)	14, 145, 193, 399, 462
PpuMI	RGGWCCY	2 cut(s)	143, 198
PshAI	GACNNNNGTC	1 cut(s)	155
Psp5II	RGGWCCY	2 cut(s)	143, 198
Psp6I	CCWGG	3 cut(s)	433, 1069, 1493
PspFI	CCCAGC	1 cut(s)	277
PspGI	CCWGG	3 cut(s)	433, 1069, 1493
PspN4I	GGNNCC	7 cut(s)	144, 145, 223, 244, 310, 1298, 1500
PspPI	GGNCC	5 cut(s)	109, 143, 198, 243, 944
PspPPI	RGGWCCY	2 cut(s)	143, 198
PstNI	CAGNNNCTG	1 cut(s)	766
PsuI	RGATCY	1 cut(s)	125
PvuII	CAGCTG	1 cut(s)	281
RsaI	GTAC	3 cut(s)	530, 1274, 1557
RsaNI	GTAC	3 cut(s)	529, 1273, 1556
RseI	CAYNNNNRTG	5 cut(s)	583, 690, 812, 894, 1416
SacII	CCGCGG	2 cut(s)	95, 105
SaqAI	TTAA	6 cut(s)	342, 426, 599, 771, 1478, 1591
Sau3AI	GATC	5 cut(s)	125, 934, 1033, 1240, 1543
Sau96I	GGNCC	5 cut(s)	109, 143, 198, 243, 944
SchI	GAGTC	5 cut(s)	14, 145, 194, 399, 463
ScrFI	CCNGG	6 cut(s)	140, 141, 402, 435, 1071, 1495
SduI	GDGCHC	4 cut(s)	140, 233, 809, 1501
SfaNI	GCATC	4 cut(s)	689, 1114, 1178, 1478
SfcI	CTRYAG	2 cut(s)	1258, 1514
Sfr274I	CTCGAG	1 cut(s)	160
Sfr303I	CCGCGG	2 cut(s)	95, 105
SgrAI	CRCCGGYG	1 cut(s)	235
SgrBI	CCGCGG	2 cut(s)	95, 105
SinI	GGWCC	3 cut(s)	143, 198, 243
SlaI	CTCGAG	1 cut(s)	160
SmaI	CCCGGG	1 cut(s)	141
SmiMI	CAYNNNNRTG	5 cut(s)	583, 690, 812, 894, 1416
SmlI	CTYRAG	2 cut(s)	160, 501
SmoI	CTYRAG	2 cut(s)	160, 501
Sse9I	AATT	3 cut(s)	782, 817, 1077
SseBI	AGGCCT	2 cut(s)	482, 724
SspMI	CTAG	2 cut(s)	852, 1110
StuI	AGGCCT	2 cut(s)	482, 724
StyD4I	CCNGG	6 cut(s)	138, 139, 400, 433, 1069, 1493
StyI	CCWWGG	3 cut(s)	225, 483, 1307
TaaI	ACNGT	3 cut(s)	394, 1065, 1476
TaiI	ACGT	2 cut(s)	1164, 1331
TaqI	TCGA	6 cut(s)	23, 161, 171, 790, 840, 1248
TasI	AATT	3 cut(s)	782, 817, 1077
TatI	WGTACW	1 cut(s)	528
TauI	GCSGC	6 cut(s)	52, 94, 97, 123, 382, 1055
TfiI	GAWTC	2 cut(s)	983, 1040
Tru1I	TTAA	6 cut(s)	342, 426, 599, 771, 1478, 1591
Tru9I	TTAA	6 cut(s)	342, 426, 599, 771, 1478, 1591
TscAI	CASTG	5 cut(s)	81, 721, 784, 1378, 1479
TseFI	GTSAC	2 cut(s)	468, 1198
TseI	GCWGC	6 cut(s)	61, 506, 563, 622, 1430, 1443
Tsp45I	GTSAC	2 cut(s)	468, 1198
TspDTI	ATGAA	7 cut(s)	272, 699, 830, 912, 1101, 1146, 1416
TspGWI	ACGGA	3 cut(s)	447, 975, 1337
TspMI	CCCGGG	1 cut(s)	139
TspRI	CASTG	5 cut(s)	81, 721, 784, 1378, 1479
Van91I	CCANNNNNTGG	2 cut(s)	284, 929
VneI	GTGCAC	1 cut(s)	805
VpaK11BI	GGWCC	3 cut(s)	143, 198, 243
XagI	CCTNNNNNAGG	1 cut(s)	1517
XapI	RAATTY	1 cut(s)	782
XceI	RCATGY	3 cut(s)	535, 588, 1358
XhoI	CTCGAG	1 cut(s)	160
XmaI	CCCGGG	1 cut(s)	139
XspI	CTAG	2 cut(s)	852, 1110

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.