MD09G1058900.v1.1

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012172
Physical Location & Seq
Forward (+)
3901644 .. 3902501
858 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1058900.v1.1.491

Sequence Viewer

Length: 636 bp
ATGATAAGTAGCCGTGGGGAGTCATCTATGCCGTCCAACGATGATCGCAAACCTTCCATTGGTGCCGATTGGCGATCATTCAGAGCAAAACTGGTAGCTGCAGAGAAATCAAGGCCTAAAGAGCCCACTTCAGCTTCCTCACTTGTTGATCTTCACACTCTGGTGGACCAACCTAATCCCATCACTGTTGGTGACAAATGTGCTCACACAATCCACAAGTCCGAAAAGGGCTGCCTACTCCTTGCAACCAAAAACCACGGCGGGGTCCACATTTTCGAGCGGACGATCATTTGGGTGTTGTCCACTGGACCTTTGGGCCCATCCGGCATCATTCTCAACCGGCCTTCCCTCATGTCGAACAAGGAAACTCGATGGACCGCTCGAGACGTGGCGGGCACGTTCTCGGACCGGCCGTTGTTCTTCGGCGCGCCGTTGGAGGAGGGGTTGTTCTTGGTGAGGCCCAAAGGGGGTGATCATGCGGTTGGGAGGGAACGTGTGGGGTGTGCTGCTGAAATAGTGAAAAGGGATACGGTTGGGCTTGGTGAGTTTCGGTTTTTTTATGGATATTGTGGGTGGGAGCAAGGGCAATTAAAGAATGAAATTAAAGTTGGTTATTGGACTGTGGCAAGCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179

Protein Analysis

212

Amino Acids

23.17

Weight (kDa)

9.1

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 91 - 210 1.5e-16 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29240 AT3G29240
fragaria_vesca FvH4_6g47750
malus_domestica MD09G1058900.v1.1 MD17G1054800.v1.1
prunus_persica Prupe.3G261300_v2.0.a1
pyrus_communis pycom17g05230
rosa_chinensis RchiOBHm_Chr2g0166901
rosa_laevigata RLG00000021677
rosa_multiflora Rmu_sc0002735.1_g000015 Rmu_sc0005344.1_g000008
rosa_roxburghii Rroxscaffold_2G00084030 Rroxscaffold_2G00084430
rosa_rugosa Rorug02G0526200
rosa_samantha Rh2AG593200 Rh2BG604700 Rh2CG575400 Rh2DG616200
rosa_wichuraiana Rw2G049380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 62
AccBSI CCGCTC 2 cut(s) 280, 380
AccII CGCG 1 cut(s) 428
AciI CCGC 5 cut(s) 261, 280, 378, 392, 479
AcoI YGGCCR 1 cut(s) 410
AcuI CTGAAG 1 cut(s) 114
AfiI CCNNNNNNNGG 3 cut(s) 59, 262, 467
AflIII ACRYGT 1 cut(s) 493
AjiI CACGTC 1 cut(s) 388
AjuI GAANNNNNNNTTGG 2 cut(s) 591, 623
AleI CACNNNNGTG 1 cut(s) 161
AloI GAACNNNNNNTCC 2 cut(s) 431, 463
AluBI AGCT 3 cut(s) 98, 134, 630
AluI AGCT 3 cut(s) 98, 134, 630
Alw21I GWGCWC 1 cut(s) 205
Alw26I GTCTC 1 cut(s) 378
Ama87I CYCGRG 1 cut(s) 381
AoxI GGCC 5 cut(s) 113, 316, 341, 410, 458
ApaI GGGCCC 1 cut(s) 320
ApeKI GCWGC 3 cut(s) 98, 231, 506
AscI GGCGCGCC 1 cut(s) 426
AspLEI GCGC 2 cut(s) 428, 430
AspS9I GGNCC 8 cut(s) 166, 265, 308, 316, 317, 375, 406, 459
AsuHPI GGTGA 4 cut(s) 203, 466, 482, 554
AvaI CYCGRG 1 cut(s) 381
AvaII GGWCC 5 cut(s) 166, 265, 308, 375, 406
BaeGI GKGCMC 2 cut(s) 320, 398
BanI GGYRCC 1 cut(s) 62
BanII GRGCYC 2 cut(s) 126, 320
Bbv12I GWGCWC 1 cut(s) 205
BbvI GCAGC 3 cut(s) 85, 218, 493
BccI CCATC 3 cut(s) 188, 328, 366
BceAI ACGGC 4 cut(s) 16, 274, 397, 415
BciVI GTATCC 1 cut(s) 520
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 99
BfuI GTATCC 1 cut(s) 520
BglI GCCNNNNNGGC 1 cut(s) 324
BisI GCNGC 3 cut(s) 99, 232, 507
BlsI GCNGC 3 cut(s) 100, 233, 508
Bme18I GGWCC 5 cut(s) 166, 265, 308, 375, 406
BmeT110I CYCGRG 1 cut(s) 381
BmgBI CACGTC 1 cut(s) 388
BmgT120I GGNCC 8 cut(s) 166, 265, 308, 316, 317, 375, 406, 459
BmiI GGNNCC 3 cut(s) 64, 266, 318
BmsI GCATC 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 13, 256
BsaXI ACNNNNNCTCC 6 cut(s) 428, 431, 458, 461, 478, 508
Bsc4I CCNNNNNNNGG 3 cut(s) 59, 262, 467
Bse118I RCCGGY 2 cut(s) 339, 408
Bse1I ACTGG 2 cut(s) 96, 310
BseDI CCNNGG 2 cut(s) 13, 256
BseGI GGATG 1 cut(s) 320
BseLI CCNNNNNNNGG 3 cut(s) 59, 262, 467
BseNI ACTGG 2 cut(s) 96, 310
BsePI GCGCGC 1 cut(s) 426
BseRI GAGGAG 1 cut(s) 452
BseSI GKGCMC 2 cut(s) 320, 398
BseX3I CGGCCG 1 cut(s) 410
BseXI GCAGC 3 cut(s) 85, 218, 493
Bsh1236I CGCG 1 cut(s) 428
Bsh1285I CGRYCG 1 cut(s) 413
BshFI GGCC 5 cut(s) 115, 318, 343, 412, 460
BshNI GGYRCC 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 205
BsiHKCI CYCGRG 1 cut(s) 381
BsiSI CCGG 3 cut(s) 324, 340, 409
BslI CCNNNNNNNGG 3 cut(s) 59, 262, 467
BsmAI GTCTC 1 cut(s) 378
BsmBI CGTCTC 1 cut(s) 378
BsnI GGCC 5 cut(s) 115, 318, 343, 412, 460
BsoBI CYCGRG 1 cut(s) 381
Bsp120I GGGCCC 1 cut(s) 316
Bsp1286I GDGCHC 4 cut(s) 126, 205, 320, 398
Bsp143I GATC 5 cut(s) 43, 74, 148, 285, 472
BspACI CCGC 5 cut(s) 261, 280, 378, 392, 479
BspANI GGCC 5 cut(s) 115, 318, 343, 412, 460
BspFNI CGCG 1 cut(s) 428
BspLI GGNNCC 3 cut(s) 64, 266, 318
BspMAI CTGCAG 1 cut(s) 103
BspT107I GGYRCC 1 cut(s) 62
BsrBI CCGCTC 2 cut(s) 280, 380
BsrFI RCCGGY 2 cut(s) 339, 408
BsrI ACTGG 2 cut(s) 96, 310
BssAI RCCGGY 2 cut(s) 339, 408
BssECI CCNNGG 2 cut(s) 13, 256
BssHII GCGCGC 1 cut(s) 426
BssMI GATC 5 cut(s) 43, 74, 148, 285, 472
Bst4CI ACNGT 3 cut(s) 187, 532, 622
BstC8I GCNNGC 3 cut(s) 394, 428, 628
BstDSI CCRYGG 2 cut(s) 13, 256
BstF5I GGATG 1 cut(s) 320
BstFNI CGCG 1 cut(s) 428
BstHHI GCGC 2 cut(s) 428, 430
BstKTI GATC 5 cut(s) 46, 77, 151, 288, 475
BstMAI GTCTC 1 cut(s) 378
BstMBI GATC 5 cut(s) 43, 74, 148, 285, 472
BstMCI CGRYCG 1 cut(s) 413
BstMWI GCNNNNNNNGC 2 cut(s) 121, 324
BstSFI CTRYAG 1 cut(s) 99
BstSLI GKGCMC 2 cut(s) 320, 398
BstUI CGCG 1 cut(s) 428
BstV1I GCAGC 3 cut(s) 85, 218, 493
BstZI CGGCCG 1 cut(s) 410
BsuI GTATCC 1 cut(s) 520
BsuRI GGCC 5 cut(s) 115, 318, 343, 412, 460
BtgI CCRYGG 2 cut(s) 13, 256
BtrI CACGTC 1 cut(s) 388
BtsCI GGATG 1 cut(s) 320
BtsIMutI CAGTG 2 cut(s) 183, 303
Cac8I GCNNGC 3 cut(s) 394, 428, 628
CfoI GCGC 2 cut(s) 428, 430
Cfr10I RCCGGY 2 cut(s) 339, 408
Cfr13I GGNCC 8 cut(s) 166, 265, 308, 316, 317, 375, 406, 459
CpoI CGGWCCG 1 cut(s) 406
CspI CGGWCCG 1 cut(s) 406
CviAII CATG 2 cut(s) 352, 476
DpnI GATC 5 cut(s) 45, 76, 150, 287, 474
DpnII GATC 5 cut(s) 43, 74, 148, 285, 472
EaeI YGGCCR 1 cut(s) 410
EagI CGGCCG 1 cut(s) 410
EclXI CGGCCG 1 cut(s) 410
Eco147I AGGCCT 1 cut(s) 115
Eco24I GRGCYC 2 cut(s) 126, 320
Eco47I GGWCC 5 cut(s) 166, 265, 308, 375, 406
Eco52I CGGCCG 1 cut(s) 410
Eco57I CTGAAG 1 cut(s) 114
Eco88I CYCGRG 1 cut(s) 381
EcoT38I GRGCYC 2 cut(s) 126, 320
Esp3I CGTCTC 1 cut(s) 378
FaeI CATG 2 cut(s) 355, 479
FaiI YATR 4 cut(s) 29, 353, 477, 561
FatI CATG 2 cut(s) 351, 475
FauI CCCGC 2 cut(s) 254, 385
FbaI TGATCA 1 cut(s) 472
Fnu4HI GCNGC 3 cut(s) 99, 232, 507
FokI GGATG 1 cut(s) 307
FriOI GRGCYC 2 cut(s) 126, 320
Fsp4HI GCNGC 3 cut(s) 99, 232, 507
GlaI GCGC 2 cut(s) 427, 429
GluI GCNGC 3 cut(s) 99, 232, 507
HaeIII GGCC 5 cut(s) 115, 318, 343, 412, 460
HapII CCGG 3 cut(s) 324, 340, 409
HhaI GCGC 2 cut(s) 428, 430
Hin1II CATG 2 cut(s) 355, 479
Hin6I GCGC 2 cut(s) 426, 428
HinP1I GCGC 2 cut(s) 426, 428
HindIII AAGCTT 1 cut(s) 628
HinfI GANTC 1 cut(s) 20
HpaII CCGG 3 cut(s) 324, 340, 409
HphI GGTGA 4 cut(s) 203, 466, 482, 554
Hpy166II GTNNAC 3 cut(s) 166, 268, 303
Hpy188I TCNGA 3 cut(s) 83, 223, 406
Hpy188III TCNNGA 1 cut(s) 383
Hpy8I GTNNAC 3 cut(s) 166, 268, 303
HpyAV CCTTC 2 cut(s) 63, 354
HpyCH4III ACNGT 3 cut(s) 187, 532, 622
HpyCH4IV ACGT 3 cut(s) 387, 398, 493
HpyCH4V TGCA 2 cut(s) 101, 245
HpyF10VI GCNNNNNNNGC 2 cut(s) 121, 324
HpySE526I ACGT 3 cut(s) 387, 398, 493
Hsp92II CATG 2 cut(s) 355, 479
HspAI GCGC 2 cut(s) 426, 428
Ksp22I TGATCA 1 cut(s) 472
Kzo9I GATC 5 cut(s) 43, 74, 148, 285, 472
LmnI GCTCC 1 cut(s) 577
LpnPI CCDG 6 cut(s) 77, 146, 291, 337, 353, 422
Lsp1109I GCAGC 3 cut(s) 85, 218, 493
LweI GCATC 1 cut(s) 336
MaeII ACGT 3 cut(s) 387, 398, 493
MaeIII GTNAC 1 cut(s) 191
MalI GATC 5 cut(s) 45, 76, 150, 287, 474
MbiI CCGCTC 2 cut(s) 280, 380
MboI GATC 5 cut(s) 43, 74, 148, 285, 472
MboII GAAGA 2 cut(s) 143, 412
MhlI GDGCHC 4 cut(s) 126, 205, 320, 398
MluCI AATT 2 cut(s) 587, 600
MlyI GAGTC 1 cut(s) 29
MmeI TCCRAC 2 cut(s) 60, 414
MnlI CCTC 6 cut(s) 148, 359, 430, 433, 450, 480
MseI TTAA 2 cut(s) 590, 603
MslI CAYNNNNRTG 2 cut(s) 161, 293
MspI CCGG 3 cut(s) 324, 340, 409
MvnI CGCG 1 cut(s) 428
MwoI GCNNNNNNNGC 2 cut(s) 121, 324
NdeII GATC 5 cut(s) 43, 74, 148, 285, 472
NlaIII CATG 2 cut(s) 355, 479
NlaIV GGNNCC 3 cut(s) 64, 266, 318
NmuCI GTSAC 1 cut(s) 191
OliI CACNNNNGTG 1 cut(s) 161
PaeR7I CTCGAG 1 cut(s) 381
PalAI GGCGCGCC 1 cut(s) 426
PauI GCGCGC 1 cut(s) 426
PceI AGGCCT 1 cut(s) 115
PcsI WCGNNNNNNNCGW 1 cut(s) 410
PkrI GCNGC 3 cut(s) 100, 233, 508
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PspN4I GGNNCC 3 cut(s) 64, 266, 318
PspOMI GGGCCC 1 cut(s) 316
PspPI GGNCC 8 cut(s) 166, 265, 308, 316, 317, 375, 406, 459
PstI CTGCAG 1 cut(s) 103
PteI GCGCGC 1 cut(s) 426
RseI CAYNNNNRTG 2 cut(s) 161, 293
Rsr2I CGGWCCG 1 cut(s) 406
RsrII CGGWCCG 1 cut(s) 406
SaqAI TTAA 2 cut(s) 590, 603
SatI GCNGC 3 cut(s) 99, 232, 507
Sau3AI GATC 5 cut(s) 43, 74, 148, 285, 472
Sau96I GGNCC 8 cut(s) 166, 265, 308, 316, 317, 375, 406, 459
SchI GAGTC 1 cut(s) 29
SduI GDGCHC 4 cut(s) 126, 205, 320, 398
SetI ASST 9 cut(s) 55, 100, 136, 175, 313, 390, 401, 496, 632
SfaNI GCATC 1 cut(s) 336
SfcI CTRYAG 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 381
SgsI GGCGCGCC 1 cut(s) 426
SinI GGWCC 5 cut(s) 166, 265, 308, 375, 406
SlaI CTCGAG 1 cut(s) 381
SmiMI CAYNNNNRTG 2 cut(s) 161, 293
SmlI CTYRAG 1 cut(s) 381
SmoI CTYRAG 1 cut(s) 381
Sse9I AATT 2 cut(s) 587, 600
SseBI AGGCCT 1 cut(s) 115
SsiI CCGC 5 cut(s) 261, 280, 378, 392, 479
StuI AGGCCT 1 cut(s) 115
TaaI ACNGT 3 cut(s) 187, 532, 622
TaiI ACGT 3 cut(s) 390, 401, 496
TaqI TCGA 4 cut(s) 276, 356, 370, 382
TasI AATT 2 cut(s) 587, 600
Tru1I TTAA 2 cut(s) 590, 603
Tru9I TTAA 2 cut(s) 590, 603
TscAI CASTG 2 cut(s) 190, 310
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 3 cut(s) 98, 231, 506
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 1 cut(s) 612
TspRI CASTG 2 cut(s) 190, 310
VpaK11BI GGWCC 5 cut(s) 166, 265, 308, 375, 406
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XhoI CTCGAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.