MD17G1054800.v1.1

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012172
Physical Location & Seq
Forward (+)
4227375 .. 4228678
1304 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1054800.v1.1.491

Sequence Viewer

Length: 873 bp
ATGGAAGCTTGTTTTCTTGCCTCAAATTCCTTCACCAAAACTGTTACTTCTCCCAACAGAAATTTCACTCAATTTCAATCCAAAAGAGTTGGGATTCCATCTTCAATAACATGTAGCCGCGGGGAGTCATCAACGCCGTCCAACGATGATCACAAACCTTCCATTGATGCAGATTGGCGATCATTCAGAGCAAGACTGGTAGCTGCAGAGAAATCAAGGCCAAAAGAGCCCACTTCAACTTCCTCACTTGATCTTGACACTGTGGTGGATCAGCCTAAGCCCGTCACCGTCGGTGACAAATGGGCTCACGCAATCCACGAGCCTGAAAAGGGCTGCCTACTCCTTGCAACCGAAAAGCTCGACGGGGTCCACATATTCGAACGGACGGTCATTCTGGTGTTGTCCACTGGGCCATTGGGACCGTGGGGCATCATACTCAACCGGCCCTCCCTCATGTCGATCAAGGAAACGCGATCAACCGCTCTGGACGTGGCGGGCACGTTCTCGGACCGGCCGTTGTTCTTCGGCGGGCCGTTGGAGGAGGGGTTGTTCTTGGTGAGGCCCAAAGGGGGTGATGGTGTAGTTGGGAGGAGTGGAGTTTTTGATGAGGTGATGAAGGGGTTGTACTATGGGACAAAGGAAAGTGTGGGGTGTGCGGCTGAGATGGTGAAGAGGAACATGGTTGGGCTTGGGGAGTTTAGGTTTTTTGATGGGTATTGTGGGTGGGAGAAAGAGCAGTTGAAGGATGAAATAACATCTGGTTATTGGACTGTGGCTGCTTGTAGCCCAAGTGTGATTGATTTGAGGAGTGTGGGAAGTGTTGGCCTTTGGGAGAAGGTCCTTGGGCTTATGGGCCGTCGGAAGGTCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179

Protein Analysis

291

Amino Acids

31.75

Weight (kDa)

8.17

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 125 - 285 1.3e-25 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29240 AT3G29240
fragaria_vesca FvH4_6g47750
malus_domestica MD09G1058900.v1.1 MD17G1054800.v1.1
prunus_persica Prupe.3G261300_v2.0.a1
pyrus_communis pycom17g05230
rosa_chinensis RchiOBHm_Chr2g0166901
rosa_laevigata RLG00000021677
rosa_multiflora Rmu_sc0002735.1_g000015 Rmu_sc0005344.1_g000008
rosa_roxburghii Rroxscaffold_2G00084030 Rroxscaffold_2G00084430
rosa_rugosa Rorug02G0526200
rosa_samantha Rh2AG593200 Rh2BG604700 Rh2CG575400 Rh2DG616200
rosa_wichuraiana Rw2G049380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 482
AccII CGCG 2 cut(s) 120, 472
AciI CCGC 6 cut(s) 118, 120, 480, 494, 528, 656
AclWI GGATC 1 cut(s) 276
AcoI YGGCCR 1 cut(s) 512
AcsI RAATTY 2 cut(s) 25, 61
AfaI GTAC 1 cut(s) 626
AfiI CCNNNNNNNGG 3 cut(s) 329, 569, 862
AflIII ACRYGT 1 cut(s) 110
AgsI TTSAA 4 cut(s) 77, 105, 237, 742
AjiI CACGTC 1 cut(s) 490
AjuI GAANNNNNNNTTGG 2 cut(s) 47, 79
AleI CACNNNNGTG 1 cut(s) 263
AloI GAACNNNNNNTCC 2 cut(s) 533, 565
AluBI AGCT 3 cut(s) 8, 203, 358
AluI AGCT 3 cut(s) 8, 203, 358
AlwI GGATC 1 cut(s) 276
AoxI GGCC 8 cut(s) 218, 410, 443, 512, 530, 560, 823, 853
ApeKI GCWGC 3 cut(s) 203, 333, 776
ApoI RAATTY 2 cut(s) 25, 61
ArsI GACNNNNNNTTYG 2 cut(s) 372, 404
AsuHPI GGTGA 7 cut(s) 25, 277, 305, 568, 584, 622, 679
AsuII TTCGAA 1 cut(s) 378
AvaII GGWCC 5 cut(s) 367, 419, 508, 838, 865
BaeGI GKGCMC 1 cut(s) 500
BanII GRGCYC 2 cut(s) 231, 307
BarI GAAGNNNNNNTAC 2 cut(s) 608, 640
BauI CACGAG 1 cut(s) 317
BbvI GCAGC 3 cut(s) 190, 320, 763
BccI CCATC 4 cut(s) 106, 569, 658, 704
BceAI ACGGC 4 cut(s) 121, 499, 517, 840
BclI TGATCA 1 cut(s) 148
BfmI CTRYAG 1 cut(s) 204
BisI GCNGC 5 cut(s) 118, 204, 334, 657, 777
BlsI GCNGC 5 cut(s) 119, 205, 335, 658, 778
Bme18I GGWCC 5 cut(s) 367, 419, 508, 838, 865
BmgBI CACGTC 1 cut(s) 490
BmiI GGNNCC 2 cut(s) 368, 420
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 2 cut(s) 157, 438
BmuI ACTGGG 1 cut(s) 417
Bpu10I CCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 378
BsaJI CCNNGG 3 cut(s) 118, 422, 841
BsaWI WCCGGW 1 cut(s) 867
BsaXI ACNNNNNCTCC 8 cut(s) 431, 461, 530, 533, 560, 563, 686, 716
Bsc4I CCNNNNNNNGG 3 cut(s) 329, 569, 862
Bse118I RCCGGY 2 cut(s) 441, 510
Bse1I ACTGG 2 cut(s) 201, 412
BseDI CCNNGG 3 cut(s) 118, 422, 841
BseGI GGATG 1 cut(s) 751
BseLI CCNNNNNNNGG 3 cut(s) 329, 569, 862
BseMII CTCAG 1 cut(s) 651
BseNI ACTGG 2 cut(s) 201, 412
BseRI GAGGAG 3 cut(s) 554, 604, 820
BseSI GKGCMC 1 cut(s) 500
BseX3I CGGCCG 1 cut(s) 512
BseXI GCAGC 3 cut(s) 190, 320, 763
Bsh1236I CGCG 2 cut(s) 120, 472
Bsh1285I CGRYCG 1 cut(s) 515
BshFI GGCC 8 cut(s) 220, 412, 445, 514, 532, 562, 825, 855
BsiEI CGRYCG 1 cut(s) 515
BsiSI CCGG 3 cut(s) 442, 511, 868
BslFI GGGAC 2 cut(s) 432, 646
BslI CCNNNNNNNGG 3 cut(s) 329, 569, 862
BsmFI GGGAC 2 cut(s) 432, 646
BsnI GGCC 8 cut(s) 220, 412, 445, 514, 532, 562, 825, 855
Bsp119I TTCGAA 1 cut(s) 378
Bsp1286I GDGCHC 3 cut(s) 231, 307, 500
Bsp143I GATC 6 cut(s) 148, 179, 250, 268, 459, 473
BspACI CCGC 6 cut(s) 118, 120, 480, 494, 528, 656
BspANI GGCC 8 cut(s) 220, 412, 445, 514, 532, 562, 825, 855
BspCNI CTCAG 1 cut(s) 652
BspFNI CGCG 2 cut(s) 120, 472
BspLI GGNNCC 2 cut(s) 368, 420
BspMAI CTGCAG 1 cut(s) 208
BspPI GGATC 1 cut(s) 276
BspT104I TTCGAA 1 cut(s) 378
BsrBI CCGCTC 1 cut(s) 482
BsrFI RCCGGY 2 cut(s) 441, 510
BsrI ACTGG 2 cut(s) 201, 412
BssAI RCCGGY 2 cut(s) 441, 510
BssECI CCNNGG 3 cut(s) 118, 422, 841
BssMI GATC 6 cut(s) 148, 179, 250, 268, 459, 473
BssSI CACGAG 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 841
Bst2BI CACGAG 1 cut(s) 317
Bst4CI ACNGT 6 cut(s) 43, 262, 289, 388, 423, 772
Bst6I CTCTTC 1 cut(s) 665
BstBI TTCGAA 1 cut(s) 378
BstC8I GCNNGC 2 cut(s) 496, 530
BstDEI CTNAG 2 cut(s) 276, 660
BstDSI CCRYGG 2 cut(s) 118, 422
BstF5I GGATG 1 cut(s) 751
BstFNI CGCG 2 cut(s) 120, 472
BstKTI GATC 6 cut(s) 151, 182, 253, 271, 462, 476
BstMBI GATC 6 cut(s) 148, 179, 250, 268, 459, 473
BstMCI CGRYCG 1 cut(s) 515
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNSI RCATGY 1 cut(s) 114
BstSFI CTRYAG 1 cut(s) 204
BstSLI GKGCMC 1 cut(s) 500
BstUI CGCG 2 cut(s) 120, 472
BstV1I GCAGC 3 cut(s) 190, 320, 763
BstZI CGGCCG 1 cut(s) 512
BsuRI GGCC 8 cut(s) 220, 412, 445, 514, 532, 562, 825, 855
BtgI CCRYGG 2 cut(s) 118, 422
BtrI CACGTC 1 cut(s) 490
BtsCI GGATG 1 cut(s) 751
BtsIMutI CAGTG 2 cut(s) 258, 405
Cac8I GCNNGC 2 cut(s) 496, 530
Cfr10I RCCGGY 2 cut(s) 441, 510
Cfr42I CCGCGG 1 cut(s) 121
CpoI CGGWCCG 1 cut(s) 508
Csp6I GTAC 1 cut(s) 625
CspI CGGWCCG 1 cut(s) 508
CviAII CATG 3 cut(s) 111, 454, 679
CviQI GTAC 1 cut(s) 625
DdeI CTNAG 2 cut(s) 276, 660
DpnI GATC 6 cut(s) 150, 181, 252, 270, 461, 475
DpnII GATC 6 cut(s) 148, 179, 250, 268, 459, 473
EaeI YGGCCR 1 cut(s) 512
EagI CGGCCG 1 cut(s) 512
Eam1104I CTCTTC 1 cut(s) 665
EarI CTCTTC 1 cut(s) 665
EclXI CGGCCG 1 cut(s) 512
Eco130I CCWWGG 1 cut(s) 841
Eco24I GRGCYC 2 cut(s) 231, 307
Eco47I GGWCC 5 cut(s) 367, 419, 508, 838, 865
Eco52I CGGCCG 1 cut(s) 512
EcoO109I RGGNCCY 1 cut(s) 838
EcoT14I CCWWGG 1 cut(s) 841
EcoT38I GRGCYC 2 cut(s) 231, 307
ErhI CCWWGG 1 cut(s) 841
FaeI CATG 3 cut(s) 114, 457, 682
FaiI YATR 7 cut(s) 112, 374, 434, 455, 630, 680, 851
FaqI GGGAC 2 cut(s) 432, 646
FatI CATG 3 cut(s) 110, 453, 678
FauI CCCGC 3 cut(s) 113, 487, 521
FbaI TGATCA 1 cut(s) 148
Fnu4HI GCNGC 5 cut(s) 118, 204, 334, 657, 777
FokI GGATG 1 cut(s) 758
FriOI GRGCYC 2 cut(s) 231, 307
Fsp4HI GCNGC 5 cut(s) 118, 204, 334, 657, 777
GluI GCNGC 5 cut(s) 118, 204, 334, 657, 777
HaeIII GGCC 8 cut(s) 220, 412, 445, 514, 532, 562, 825, 855
HapII CCGG 3 cut(s) 442, 511, 868
Hin1II CATG 3 cut(s) 114, 457, 682
HindIII AAGCTT 1 cut(s) 6
HinfI GANTC 2 cut(s) 94, 125
HpaII CCGG 3 cut(s) 442, 511, 868
HphI GGTGA 7 cut(s) 25, 277, 305, 568, 584, 622, 679
Hpy166II GTNNAC 2 cut(s) 370, 405
Hpy188I TCNGA 3 cut(s) 188, 508, 861
Hpy188III TCNNGA 2 cut(s) 254, 485
Hpy8I GTNNAC 2 cut(s) 370, 405
Hpy99I CGWCG 3 cut(s) 293, 365, 861
HpyAV CCTTC 6 cut(s) 40, 168, 610, 736, 829, 856
HpyCH4III ACNGT 6 cut(s) 43, 262, 289, 388, 423, 772
HpyCH4IV ACGT 2 cut(s) 489, 500
HpyCH4V TGCA 3 cut(s) 170, 206, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 2 cut(s) 276, 660
HpySE526I ACGT 2 cut(s) 489, 500
Hsp92II CATG 3 cut(s) 114, 457, 682
Ksp22I TGATCA 1 cut(s) 148
KspI CCGCGG 1 cut(s) 121
Kzo9I GATC 6 cut(s) 148, 179, 250, 268, 459, 473
LpnPI CCDG 8 cut(s) 182, 336, 380, 393, 455, 470, 524, 744
Lsp1109I GCAGC 3 cut(s) 190, 320, 763
LweI GCATC 2 cut(s) 157, 438
MaeII ACGT 2 cut(s) 489, 500
MaeIII GTNAC 3 cut(s) 43, 283, 293
MalI GATC 6 cut(s) 150, 181, 252, 270, 461, 475
MbiI CCGCTC 1 cut(s) 482
MboI GATC 6 cut(s) 148, 179, 250, 268, 459, 473
MboII GAAGA 3 cut(s) 93, 514, 682
MhlI GDGCHC 3 cut(s) 231, 307, 500
MluCI AATT 3 cut(s) 25, 61, 71
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 3 cut(s) 165, 516, 839
MslI CAYNNNNRTG 2 cut(s) 263, 395
MspA1I CMGCKG 1 cut(s) 120
MspI CCGG 3 cut(s) 442, 511, 868
MvnI CGCG 2 cut(s) 120, 472
MwoI GCNNNNNNNGC 1 cut(s) 226
NdeII GATC 6 cut(s) 148, 179, 250, 268, 459, 473
NlaIII CATG 3 cut(s) 114, 457, 682
NlaIV GGNNCC 2 cut(s) 368, 420
NmuCI GTSAC 2 cut(s) 283, 293
NspI RCATGY 1 cut(s) 114
NspV TTCGAA 1 cut(s) 378
OliI CACNNNNGTG 1 cut(s) 263
PciI ACATGT 1 cut(s) 110
PcsI WCGNNNNNNNCGW 2 cut(s) 315, 512
PfeI GAWTC 1 cut(s) 94
PflFI GACNNNGTC 1 cut(s) 365
PkrI GCNGC 5 cut(s) 119, 205, 335, 658, 778
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
PpuMI RGGWCCY 1 cut(s) 838
PscI ACATGT 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 838
PspN4I GGNNCC 2 cut(s) 368, 420
PspPPI RGGWCCY 1 cut(s) 838
PstI CTGCAG 1 cut(s) 208
PsyI GACNNNGTC 1 cut(s) 365
RsaI GTAC 1 cut(s) 626
RsaNI GTAC 1 cut(s) 625
RseI CAYNNNNRTG 2 cut(s) 263, 395
Rsr2I CGGWCCG 1 cut(s) 508
RsrII CGGWCCG 1 cut(s) 508
SacII CCGCGG 1 cut(s) 121
SatI GCNGC 5 cut(s) 118, 204, 334, 657, 777
Sau3AI GATC 6 cut(s) 148, 179, 250, 268, 459, 473
SchI GAGTC 1 cut(s) 134
SduI GDGCHC 3 cut(s) 231, 307, 500
SfaNI GCATC 2 cut(s) 157, 438
SfcI CTRYAG 1 cut(s) 204
Sfr303I CCGCGG 1 cut(s) 121
SfuI TTCGAA 1 cut(s) 378
SgrBI CCGCGG 1 cut(s) 121
SinI GGWCC 5 cut(s) 367, 419, 508, 838, 865
SmiMI CAYNNNNRTG 2 cut(s) 263, 395
Sse9I AATT 3 cut(s) 25, 61, 71
SsiI CCGC 6 cut(s) 118, 120, 480, 494, 528, 656
StyI CCWWGG 1 cut(s) 841
TaaI ACNGT 6 cut(s) 43, 262, 289, 388, 423, 772
TaiI ACGT 2 cut(s) 492, 503
TaqI TCGA 3 cut(s) 360, 378, 458
TasI AATT 3 cut(s) 25, 61, 71
TatI WGTACW 1 cut(s) 624
TauI GCSGC 2 cut(s) 120, 659
TfiI GAWTC 1 cut(s) 94
TscAI CASTG 2 cut(s) 265, 412
TseFI GTSAC 2 cut(s) 283, 293
TseI GCWGC 3 cut(s) 203, 333, 776
Tsp45I GTSAC 2 cut(s) 283, 293
TspDTI ATGAA 2 cut(s) 629, 762
TspGWI ACGGA 1 cut(s) 397
TspRI CASTG 2 cut(s) 265, 412
Tth111I GACNNNGTC 1 cut(s) 365
VpaK11BI GGWCC 5 cut(s) 367, 419, 508, 838, 865
XapI RAATTY 2 cut(s) 25, 61
XceI RCATGY 1 cut(s) 114
XcmI CCANNNNNNNNNTGG 2 cut(s) 412, 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.