RLG00000021677

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012172
Physical Location & Seq
Reverse (-)
77220959 .. 77221711
753 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021677

Sequence Viewer

Length: 753 bp
ATGGGGTCATCTTCACCGTCCGGTGATGACCACAAACGTTCCACTAATACCGATTGGCGATCATTCAGAGCAAAACTGGTAGCTGCAGAAAAAGCATCACTGCCCATAGTGCCTACTTCCCCAGTTGTTGATCCAGACACTGTGGTCGATCAACCTCAACCTATCACTGTTGGTGACAAGTGGGCTCACAAAATATACGAGCCCGAAAGAGGCTGCGTGCTCCTTGCCACCGAAAAGCTCGACGGGGTCCATATTTTCGAACGGACAGTTATTTTGGTTTTGTCCACTGGGCCTTTGGGGCTGTCAGGCATCATACTCAACCGGCCCTCCCTAATGTCGATCAAGGAAACAAGGTCGACGGCTTTAGATGTCGCCGGGACATTCTCAGACAGGCCGCTGTTCTTCGGTGGGCCTTTGGAGGAAGGGCTGTTCTTAGTGAGGCCCAAGAAGGGTGATGTTATAGTTGGGAGGAGTGGTGTTTTTGATGAGGTGATGAAGGGATTGTATTATGGGACAAAGGAAAGTGTGGGTTGTGCAGCTGAAATGGTGAAGAGGAATATGGTTGGGCTTGGGGACTTCAGGTTCTTTGATGGGCATTGTGGGTGGGAAAAGGAGCAATTGAAGAATGAGATAAGAGCTGGTTATTGGACTGTAGCAGCTTGTAGCCCAAGTGTAATTGACTTGAGTAATGTGGGAAGTGTTGGGCTTTGGGAGAAGGTTCTTGGGCTTATGGGCCGTAGAAAGGTTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179

Protein Analysis

251

Amino Acids

27.29

Weight (kDa)

8.3

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 85 - 245 4.6e-25 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29240 AT3G29240
fragaria_vesca FvH4_6g47750
malus_domestica MD09G1058900.v1.1 MD17G1054800.v1.1
prunus_persica Prupe.3G261300_v2.0.a1
pyrus_communis pycom17g05230
rosa_chinensis RchiOBHm_Chr2g0166901
rosa_laevigata RLG00000021677
rosa_multiflora Rmu_sc0002735.1_g000015 Rmu_sc0005344.1_g000008
rosa_roxburghii Rroxscaffold_2G00084030 Rroxscaffold_2G00084430
rosa_rugosa Rorug02G0526200
rosa_samantha Rh2AG593200 Rh2BG604700 Rh2CG575400 Rh2DG616200
rosa_wichuraiana Rw2G049380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 143
AccI GTMKAC 1 cut(s) 356
AciI CCGC 1 cut(s) 395
AclI AACGTT 1 cut(s) 37
AclWI GGATC 1 cut(s) 125
AcuI CTGAAG 1 cut(s) 562
AfiI CCNNNNNNNGG 3 cut(s) 209, 449, 742
AgsI TTSAA 1 cut(s) 622
AloI GAACNNNNNNTCC 4 cut(s) 413, 445, 566, 598
AluBI AGCT 5 cut(s) 83, 238, 539, 638, 659
AluI AGCT 5 cut(s) 83, 238, 539, 638, 659
Alw21I GWGCWC 1 cut(s) 222
AlwI GGATC 1 cut(s) 125
AlwNI CAGNNNCTG 1 cut(s) 140
AoxI GGCC 6 cut(s) 290, 323, 392, 410, 440, 733
ApeKI GCWGC 4 cut(s) 83, 213, 536, 656
ArsI GACNNNNNNTTYG 2 cut(s) 256, 288
AspS9I GGNCC 6 cut(s) 247, 290, 324, 410, 441, 733
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 6 cut(s) 6, 35, 185, 464, 502, 559
AsuII TTCGAA 1 cut(s) 258
AvaII GGWCC 1 cut(s) 247
BanII GRGCYC 2 cut(s) 187, 204
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 4 cut(s) 70, 200, 548, 668
BccI CCATC 1 cut(s) 584
BceAI ACGGC 2 cut(s) 375, 720
BcnI CCSGG 1 cut(s) 376
BfmI CTRYAG 2 cut(s) 84, 651
BglI GCCNNNNNGGC 1 cut(s) 298
BisI GCNGC 5 cut(s) 84, 214, 395, 537, 657
BlsI GCNGC 5 cut(s) 85, 215, 396, 538, 658
Bme1390I CCNGG 1 cut(s) 376
Bme18I GGWCC 1 cut(s) 247
BmgT120I GGNCC 6 cut(s) 247, 290, 324, 410, 441, 733
BmiI GGNNCC 1 cut(s) 248
BmrFI CCNGG 1 cut(s) 376
BmrI ACTGGG 2 cut(s) 116, 297
BmsI GCATC 2 cut(s) 104, 318
BmuI ACTGGG 2 cut(s) 116, 297
Bpu14I TTCGAA 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 703
BpuMI CCSGG 1 cut(s) 376
BsaWI WCCGGW 1 cut(s) 20
BsaXI ACNNNNNCTCC 4 cut(s) 311, 341, 460, 490
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 449, 742
Bse118I RCCGGY 1 cut(s) 321
Bse1I ACTGG 3 cut(s) 81, 122, 292
BseLI CCNNNNNNNGG 3 cut(s) 209, 449, 742
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 3 cut(s) 81, 122, 292
BseRI GAGGAG 1 cut(s) 484
BseXI GCAGC 4 cut(s) 70, 200, 548, 668
BsgI GTGCAG 1 cut(s) 555
BshFI GGCC 6 cut(s) 292, 325, 394, 412, 442, 735
BsiHKAI GWGCWC 1 cut(s) 222
BsiSI CCGG 3 cut(s) 21, 322, 375
BslFI GGGAC 3 cut(s) 391, 526, 587
BslI CCNNNNNNNGG 3 cut(s) 209, 449, 742
BsmFI GGGAC 3 cut(s) 391, 526, 587
BsnI GGCC 6 cut(s) 292, 325, 394, 412, 442, 735
Bsp119I TTCGAA 1 cut(s) 258
Bsp1286I GDGCHC 3 cut(s) 187, 204, 222
Bsp143I GATC 4 cut(s) 59, 130, 148, 339
BspACI CCGC 1 cut(s) 395
BspANI GGCC 6 cut(s) 292, 325, 394, 412, 442, 735
BspCNI CTCAG 1 cut(s) 398
BspLI GGNNCC 1 cut(s) 248
BspMAI CTGCAG 1 cut(s) 88
BspPI GGATC 1 cut(s) 125
BspT104I TTCGAA 1 cut(s) 258
BsrFI RCCGGY 1 cut(s) 321
BsrI ACTGG 3 cut(s) 81, 122, 292
BssAI RCCGGY 1 cut(s) 321
BssMI GATC 4 cut(s) 59, 130, 148, 339
Bst4CI ACNGT 5 cut(s) 18, 142, 169, 268, 652
Bst6I CTCTTC 1 cut(s) 545
BstBI TTCGAA 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 218
BstDEI CTNAG 2 cut(s) 385, 433
BstKTI GATC 4 cut(s) 62, 133, 151, 342
BstMBI GATC 4 cut(s) 59, 130, 148, 339
BstMWI GCNNNNNNNGC 3 cut(s) 92, 109, 298
BstSCI CCNGG 1 cut(s) 374
BstSFI CTRYAG 2 cut(s) 84, 651
BstV1I GCAGC 4 cut(s) 70, 200, 548, 668
BsuRI GGCC 6 cut(s) 292, 325, 394, 412, 442, 735
BtsI GCAGTG 1 cut(s) 98
BtsIMutI CAGTG 4 cut(s) 98, 138, 165, 285
Cac8I GCNNGC 1 cut(s) 218
CaiI CAGNNNCTG 1 cut(s) 140
Cfr10I RCCGGY 1 cut(s) 321
Cfr13I GGNCC 6 cut(s) 247, 290, 324, 410, 441, 733
DdeI CTNAG 2 cut(s) 385, 433
DpnI GATC 4 cut(s) 61, 132, 150, 341
DpnII GATC 4 cut(s) 59, 130, 148, 339
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
Eco24I GRGCYC 2 cut(s) 187, 204
Eco47I GGWCC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 562
EcoT38I GRGCYC 2 cut(s) 187, 204
FaiI YATR 8 cut(s) 107, 196, 252, 314, 461, 510, 560, 731
FaqI GGGAC 3 cut(s) 391, 526, 587
FblI GTMKAC 1 cut(s) 356
Fnu4HI GCNGC 5 cut(s) 84, 214, 395, 537, 657
FriOI GRGCYC 2 cut(s) 187, 204
Fsp4HI GCNGC 5 cut(s) 84, 214, 395, 537, 657
GluI GCNGC 5 cut(s) 84, 214, 395, 537, 657
HaeIII GGCC 6 cut(s) 292, 325, 394, 412, 442, 735
HapII CCGG 3 cut(s) 21, 322, 375
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HpaII CCGG 3 cut(s) 21, 322, 375
HphI GGTGA 6 cut(s) 6, 35, 185, 464, 502, 559
Hpy166II GTNNAC 2 cut(s) 285, 357
Hpy188I TCNGA 2 cut(s) 68, 388
Hpy188III TCNNGA 1 cut(s) 134
Hpy8I GTNNAC 2 cut(s) 285, 357
Hpy99I CGWCG 2 cut(s) 245, 361
HpyAV CCTTC 4 cut(s) 416, 442, 490, 709
HpyCH4III ACNGT 5 cut(s) 18, 142, 169, 268, 652
HpyCH4IV ACGT 1 cut(s) 37
HpyCH4V TGCA 2 cut(s) 86, 536
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 109, 298
HpyF3I CTNAG 2 cut(s) 385, 433
HpySE526I ACGT 1 cut(s) 37
Kzo9I GATC 4 cut(s) 59, 130, 148, 339
LmnI GCTCC 2 cut(s) 225, 613
Lsp1109I GCAGC 4 cut(s) 70, 200, 548, 668
LweI GCATC 2 cut(s) 104, 318
MaeII ACGT 1 cut(s) 37
MaeIII GTNAC 1 cut(s) 173
MalI GATC 4 cut(s) 61, 132, 150, 341
MboI GATC 4 cut(s) 59, 130, 148, 339
MboII GAAGA 4 cut(s) 3, 394, 562, 634
MfeI CAATTG 1 cut(s) 617
MhlI GDGCHC 3 cut(s) 187, 204, 222
MluCI AATT 2 cut(s) 617, 675
MnlI CCTC 8 cut(s) 165, 203, 337, 412, 432, 462, 481, 546
MspA1I CMGCKG 2 cut(s) 397, 539
MspI CCGG 3 cut(s) 21, 322, 375
MspR9I CCNGG 1 cut(s) 376
MunI CAATTG 1 cut(s) 617
MwoI GCNNNNNNNGC 3 cut(s) 92, 109, 298
NciI CCSGG 1 cut(s) 376
NdeII GATC 4 cut(s) 59, 130, 148, 339
NlaIV GGNNCC 1 cut(s) 248
NmuCI GTSAC 1 cut(s) 173
NspV TTCGAA 1 cut(s) 258
PflFI GACNNNGTC 1 cut(s) 245
PkrI GCNGC 5 cut(s) 85, 215, 396, 538, 658
Psp1406I AACGTT 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 248
PspPI GGNCC 6 cut(s) 247, 290, 324, 410, 441, 733
PstI CTGCAG 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 140
PsyI GACNNNGTC 1 cut(s) 245
PvuII CAGCTG 1 cut(s) 539
SalI GTCGAC 1 cut(s) 355
SatI GCNGC 5 cut(s) 84, 214, 395, 537, 657
Sau3AI GATC 4 cut(s) 59, 130, 148, 339
Sau96I GGNCC 6 cut(s) 247, 290, 324, 410, 441, 733
ScrFI CCNGG 1 cut(s) 376
SduI GDGCHC 3 cut(s) 187, 204, 222
SfaNI GCATC 2 cut(s) 104, 318
SfcI CTRYAG 2 cut(s) 84, 651
SfuI TTCGAA 1 cut(s) 258
SinI GGWCC 1 cut(s) 247
SmlI CTYRAG 1 cut(s) 682
SmoI CTYRAG 1 cut(s) 682
Sse9I AATT 2 cut(s) 617, 675
SsiI CCGC 1 cut(s) 395
StyD4I CCNGG 1 cut(s) 374
TaaI ACNGT 5 cut(s) 18, 142, 169, 268, 652
TaiI ACGT 1 cut(s) 40
TaqI TCGA 5 cut(s) 147, 240, 258, 338, 356
TasI AATT 2 cut(s) 617, 675
TauI GCSGC 1 cut(s) 397
TscAI CASTG 4 cut(s) 105, 145, 172, 292
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 4 cut(s) 83, 213, 536, 656
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 1 cut(s) 509
TspGWI ACGGA 1 cut(s) 277
TspRI CASTG 4 cut(s) 105, 145, 172, 292
Tth111I GACNNNGTC 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 247
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
XmiI GTMKAC 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.