MD09G1064300.v1.1

Sister chromatid cohesion protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0010273
Physical Location & Seq
Reverse (-)
4401579 .. 4413614
12036 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1064300.v1.1.491

Sequence Viewer

Length: 5427 bp
ATGAGTTATCCGGTAGCCACTTCGAGCTCGAGCTCCGGCTCGGCTCACGGAGGTATCAGCCTCTCGAACACGGTGCACTCGGAGGTCGCGCCTTGCTTGCCTCTGCCGTCGCTGCCGGTGTTCTGCGGGGCATCTGATCACCTCCGGCTGTTCGACGAACCCAGTCGGAACAGTGCGTGGCTGAACCGCCCTGACGCAGCTGTTGCGAGTAGGATTGCTGATCTGCTTCGAGAAACCGACGTGTCGTACTTGAACCTTAGGGAGGATGCTAGGGCAATTCCATATGGTTATGGAGAACCTTTAAAACTTCACGATGAAGTTCTTCGGTACAATCCAGAAGCATTTGAGTACTATAGACCAGGTCCTATTAAGGGGCAAGTGCCTGTCAGTAGTACAGTATCTGACAAGAAGCCCTTCAAGCCTATTGTACCTATACCAAAGCTTTCTCAAAGAGACTATTCCGTATCTGACAATAATCAGCTCAATGATATTCCTCCTAATGACATATCTCCACCATCTTCTAGAAAACCAAAATCCAAGAAAAAAGCTAGTGATACCGTTGTGTCAACAGTCGGACTCGATTCTGCTGAAATTCAAGATGCCACCATCAGAAGCTTTTGTGAGTTGGTGGAGGATTTCTGTGGCAGAGCTGAAATTTTCAGTGATGACAGGGAAGAAACTGAGTGGTTATCTATGCCTCTTTCTGATCTTAGAGTGCTTGCAAATGAAATAATGTCTCTTCGAGCAAAGAAGCTTCTACATTTGGTTCCTGCAGATACTTTTGTTAGATTGTTAAGGATTTTAGATCATCAGATACATCGAGCTGAAGGCTTGACTATAATTGAATATGACCAGTCAAATTCAGATGTGGTTTCATCAATACAATGTGCACTGGAGTCCATTCACGCAGCATTAGCAGTAATGGCTCATAACCAGATGCCAAAACAACTATATAAAGAAGAGATCATTGAAAGAATTCTGGAGTTTTCCAGGCATCAGATAATGGATGTTATGTGCGCTTATGATCCATCATATCGTGCTTTGCATAGGCCAAGTCAAAATGGGTCGCTCGAAGTTGAGGAAGATGAAGAGCCTGATGCTGAATTTGGTTCAGCAAGCAAGAAGAGACGCAGTATCAAGACTGTTAAAGTGCAGAAATCCTCATTTAACAGGGTCTCTGCTGCTGTGAATAATATTCTTCAGAAAATGTGCACCATTCTTGGTTTACTCAAGGATTTGTTGTTAATAGAAAGGTTGTCAGATGGTTGTATCTTACAACTAGTGAAAACAAGCTTTACAACATTTATGGTGGATAATATCCAGCTCTTGCAACTGAAGGCAATGGGTTTAATCAGCGCGATATTCTATTCATACACTCAGCATCGCACATATGTGATAGATGAATTAATTCAGTTGCTTTGGAAGTTGCCGGTCTCGAAGCGAGCTTTGAGAGCCTATCACCTACCTGATGAAGAGCAACGGCAGATTCAGATGATCACTGCTTTGCTGATTCAGTTGGTTCACTATAGTGCAAACCTTCCTGAATCATTAAGACAGGAATCAAGTGGTAATTCCATCTTAGAACTTTCTGTCAATGCCGATTATCCAACTAAAGGTCATGAGGCAGCAACAGAGGCATGTTGTCACTTTTGGACTCGTGTACTTCAGCGCTTTGCTAGTGCAAAGGCTCAGGAAGCTTCAGAGTTGAAAGTAATGATGGAGAATCTTGTTACTGATCTATTGACAACATTGAATTTACCGGAATATCCCGCTTCAGCTCCTATTCTGGAGGTTCTTTGTGTCTTGTTACTCCAGAATGCTGGACTGAAATCTAAAGACATAGCTGCACGTACCATGGCTATTGACCTTCTCGGCACAATTGCTGCAAGGTTGAAGCGTGATTCTGTACTTTGCAGTAGTGACAAAATCTGGATACTTCAGGAGTTGGTTAGTGACGATGGGGTTGATCAGACATATCCAAGAGATGCATGCTCAGTTTGTTTAGATGGTAGGGTTGATACAAATGTCTTTGTATGTCAAGGTTGTCAGAGAATGTTTCATGCTGATTGCATGGGAGTGAGAGAATATGAAGTTCCAAATCGAAGTTGGCACTGTCAGATATGCCTCTGCAGGAAGCAACTTCTTGTTTTACAATCCTATTGCAAGTCTCAATGCAAAGATGAAGGAATCAAGGATCGAAGTCGTTCAGGAAGAAACACTGAAGTTGCTTTTTCAATTACAAAACCTGAGGTTGTCCAACAGATGCTTTTGAATTACCTTCAAGATACCACATCAGCTGATGATGGGCATCTCTTTGTTCGCTGGTTTTATCTCTTGTTGTGGTACAAGGATGATTCAAAATCACAGCAGAAGCTCATGTACTATCTTGCTCGACTGAAATCAAAAGAAATAGTTCGGGATTCTGGGACTGTTTTTTCATTATTGACAAGGGATTCAGTCAAGAAAATTACTTTAGCATTGGGACAAAAAAACTCTTTCTCCAGAGGGTTTGATAAGATTCTTCATTTGCTTCTGGCAAGCTTAATGGAGAATTCTCCTGTAATCAGGGCCAAGGCTTTACGAGCGGTCAGTATAATTGTAGAAGCTGATCCACAGGTATTAGGAGATAAACTCGTTCAATCAGCTGTTGAAGGAAGATTTTGTGATTCTGCAATTTCTGCAAGAGAAGCAGCACTGGAACTTGTAGGAAGGCATATTGCTTCTCATCCTGATGTTGGTTTGCAGTATTTTGAGAAGGTTGCTGAGAGAATTAAAGATACAGGAGTAAGTGTTCGAAAGCGATCCATCAAAATCATACGAGATATGTGCATTTCAAATGCAAACTTCTCCGAATTTACAAGTGCCTGCATTGCTATCATCTCCCGTATTAGTGATGATGAATCAAGTATACAGGATATTGTTTGCAAGACATTTTATGAGTTCTGGTTTGAGGAACCTGCTGGTTCACAAACTCATTTCTTTGGAGATGGTAGCTCTGTTCCATTGGAGGTGACTAAAAAAACTGAACAGATCGTTGAGATGTTGAGGAGGATGCCAAGTCATCAACCTCTTGTGACAGTCATTAAACGCAACTTGGCCCTTGATTTTTTTCCACAATCGGCTAAAGCTATTGGGATCAACCCTGTCTTGCTTGCATCTGTACGCAACCGCTGTGAGTTAATGTGCAAGTGCTTACTGGAAAAGATATTGCAGGTAGAGGAAATGAATATCCAGGAGGTAGATAGGCAGGCACTTCCGTATGTGCTAGCTTTGCATGCATTCTGTGTTGTGGATCCAACACTTTGTGCACCGGCTTCTGACCCTTCACAGTTTGTGGTCACTCTTCAGCCTTATCTAAAGAGTCAGGCTGATAATCGAGTGGTTGCACAGTTAGTGGAGAGTATAATCTTTATAATTGATGCTGTTCTGCCTTTGGTAAGAAAGTTACCACAAACTGTTGTGGAAGAACTTGAACAAGACTTAAAGAACATGATTGTTCGGCATTCCTTCTTGACTGTTGTGCATGCTTGCATAAAGTGCCTCTGTGCTGTGAGTAAAGTGGCAGGAAAAGGTGCTGCTATAGTGGAGAATCTTATCCAGGTGTTCTTCAAACGTTTGGATGCTCAGGCAGTAGATAACAAGCAGCAAGTGGGGCGGTCTCTTTTCTGTCTCGGATTGCTTATCCGTTACGGCAACTCTTTGCCCAGTAACTCCGACAGAACAATTGATGTTGTTAGCAGTCTCAGCTTGTTTAAAAAATATCTTCTAGTGGATGATTTTGTAATAAAGGTTCGGTCATTACAGGCATTGGGCTTTGTTCTAATAGCTAGGCCCGAATATATGTTGGAAAAAGATATTGGGAAGATAGTAGAGGCAACATTCTCTTCTAGCTCTGATGTTCGTCTTAGGATGCAAGCATTGCAAAACATGTACGACTATCTTCTTGATGCTGAAAGTAAAATGGGAACAGATGCAGCCAGTGATAATGTGATTCATTGTAATGTGGAAGGCGGCAATGCTGTATCTGTTGCTGCGGGTGCTGGAGATACTAATATCTGTGGTGGTATAGTTCAGTTGTATTGGGATAATATGTTGGTGAGATGTTTAGACCTTAATGAACAAGTTCGCCAATCTGCAGTTAAGATTGTTGAAGTTGTGCTGCGCCAAGGGCTTGTACATCCTATTACATGTGTTCCATACCTTATTGCACTCGAAACAGATCCACTGGAGGCCAACTCGAAGTTAGCTCATCATTTACTGATGCAAATGAATGAAAAGTACCCCGCATTTTTCGAAAGCCGCCTGGGAGATGGCCTTCAAATGTCTTTTAGTTTCATACAGTCCATAAGTTCTAGTTCTGAACATGAGAACAAAAAAATTCAAGCAAAGGCTTCTGGAAATGCGAAGGGAAAATCTGATAATGTTTCTCTCGAACAAGCAAGACTAGGGGTTTCTCGAATCTACAAGCTTATTCGTGCTAACCGGACTTCAAGAAACAAATTTATGTCTTCAATTGTTCGCAAGTTCGATAATACTAGCTGGACACCTTCAGTAGTCCCTTTCCTGATGTACTGCACAGAGATACTCGCCTTGCTACCCTTCACAACACCTGATGAGCCGCTTTACTTGGTCTATTCCATAAATAGAGTAGTACAAGTTAGGGCTGGGGCGCTCGAAGCAAAACTTAAAGCTCTGACTTTGCATTTGTTACAAAGAAGCGCACCTCATGGGAACGGCATAATTGAAGATGGTTCAGCTGCTCAACCTTTTACGAGAGGAATGGCGTCATTCGATTTGAATGGGACAGTTCAACAAGAACCGGTGTTTCAACCATTTACAAATTATATGTCAATGGAGTGGAATGGTATAATACAACAAGAGCCAGCAGATCAGTCTGTCTCAAATCAAGCTACCCCGTTTGAGGCAAACATGAACGTTACGGGCTCCAACAGTTCTGGTGGCTTCTCAATAGATGACGTACAGAAATTCCAGGCAGACTGCCTGGCGGCTATTGCGGTGCAGCTTCTTTTGAAATTAAAGAGACACCTAAAAATTGTGTACAGCCTGAACGATGCAAGGTGCCAGGCTTTTTCTCCGGGCGAACCCCTTAAACCGGGAGATGTACTCTCCAGGCAGAACATTCCTTTTGACCTCAGTGATACCCGCACCAATTTGCCTACAACTTATCAAGAATTGGTTCAAAGATATCAGGAATTCAAGAATGCGTTGAGGGAGGATACGATTGATTTCTCAACTTACACTGCAAACATCAAGAGAAAACGCCCAGCCCCGAGAAAAGGGCGGAAATCTGTGGGTGGAGATGACGAGGGCGATGATGACGACGAGGATTATTCAGGTGGGGCTCGGAGGCCGAGTAATATTGGAAGGAGAGGTGGCTACGGTATCAGAAGTCGACAGCGGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000070 GO:0000122 GO:0000278 GO:0000280 GO:0000428 GO:0000819 GO:0001654 GO:0001655 GO:0001656 GO:0001822 GO:0003006 GO:0003007 GO:0003008 GO:0003151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007064 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0007423 GO:0007507 GO:0007548 GO:0007600 GO:0007605 GO:0008022 GO:0008104 GO:0008150 GO:0009314 GO:0009628 GO:0009653 GO:0009790 GO:0009887 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010165 GO:0010171 GO:0010212 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010720 GO:0016043 GO:0016591 GO:0019219 GO:0019222 GO:0019899 GO:0019904 GO:0022008 GO:0022402 GO:0022414 GO:0030154 GO:0030326 GO:0030334 GO:0030335 GO:0030880 GO:0031056 GO:0031058 GO:0031063 GO:0031065 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032039 GO:0032116 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0033365 GO:0033554 GO:0034085 GO:0034086 GO:0034088 GO:0034502 GO:0034613 GO:0035107 GO:0035108 GO:0035112 GO:0035113 GO:0035115 GO:0035136 GO:0035239 GO:0035261 GO:0035295 GO:0040007 GO:0040008 GO:0040012 GO:0040017 GO:0042471 GO:0042634 GO:0042826 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043388 GO:0043583 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045597 GO:0045892 GO:0045934 GO:0045995 GO:0047485 GO:0048285 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048546 GO:0048557 GO:0048562 GO:0048565 GO:0048566 GO:0048568 GO:0048589 GO:0048592 GO:0048598 GO:0048608 GO:0048638 GO:0048699 GO:0048731 GO:0048736 GO:0048806 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0050896 GO:0050954 GO:0051094 GO:0051098 GO:0051099 GO:0051101 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051253 GO:0051270 GO:0051272 GO:0051276 GO:0051641 GO:0051716 GO:0051960 GO:0051962 GO:0055029 GO:0055123 GO:0060065 GO:0060173 GO:0060255 GO:0060284 GO:0060322 GO:0060323 GO:0060324 GO:0060325 GO:0061008 GO:0061010 GO:0061038 GO:0061458 GO:0061695 GO:0065007 GO:0065009 GO:0070013 GO:0070087 GO:0070727 GO:0071168 GO:0071214 GO:0071478 GO:0071479 GO:0071481 GO:0071840 GO:0071921 GO:0072001 GO:0072359 GO:0080090 GO:0090311 GO:0090312 GO:0090596 GO:0090694 GO:0098813 GO:0104004 GO:0140014 GO:1902275 GO:1902494 GO:1902679 GO:1903047 GO:1903506 GO:1903507 GO:1905269 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2000145 GO:2000147 GO:2001141 GO:2001222 GO:2001224 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04111 map04111
Pfam Domains
Protein Families
Cohesin_HEAT Nipped-B_C PHD

Protein Analysis

1809

Amino Acids

202.07

Weight (kDa)

6.56

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 664 - 708 4e-06 PHD-finger
Cohesin_HEAT PF12765 861 - 902 5.9e-12 HEAT repeat associated with sister chromatid cohesion
Nipped-B_C PF12830 1353 - 1550 1.2e-52 Sister chromatid cohesion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15540 AT5G15540
fragaria_vesca FvH4_6g47080 FvH4_6g47080 FvH4_6g47080
malus_domestica MD09G1064300.v1.1 MD17G1057600.v1.1
prunus_persica Prupe.3G256700_v2.0.a1
pyrus_communis pycom111g05400 pycom17g05770
rosa_chinensis RchiOBHm_Chr2g0166131
rosa_laevigata RLG00000021588
rosa_multiflora Rmu_sc0006218.1_g000028
rosa_roxburghii Rroxscaffold_2G00085180
rosa_rugosa Rorug02G0520500 Rorug02G0520600
rosa_samantha Rh2AG586600 Rh2BG598400 Rh2CG569100 Rh2DG608300
rosa_wichuraiana Rw2G048900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3413
Acc36I ACCTGC 2 cut(s) 2965, 3202
AccB1I GGYRCC 1 cut(s) 5079
AccBSI CCGCTC 1 cut(s) 2585
AccI GTMKAC 2 cut(s) 2908, 5413
AccII CGCG 2 cut(s) 89, 1358
AclI AACGTT 2 cut(s) 3613, 4935
AclWI GGATC 8 cut(s) 1019, 2202, 2603, 2796, 3143, 3287, 3300, 4214
AcyI GRCGYC 1 cut(s) 4784
AdeI CACNNNGTG 1 cut(s) 3305
AfeI AGCGCT 1 cut(s) 1670
AfiI CCNNNNNNNGG 7 cut(s) 262, 371, 1613, 2735, 4921, 5113, 5297
AflIII ACRYGT 3 cut(s) 240, 3927, 4187
AgeI ACCGGT 1 cut(s) 4819
AhlI ACTAGT 1 cut(s) 1279
AjiI CACGTC 1 cut(s) 241
AjnI CCWGG 9 cut(s) 358, 989, 3231, 3597, 4302, 4989, 5001, 5082, 5129
AjuI GAANNNNNNNTTGG 2 cut(s) 3840, 3872
AleI CACNNNNGTG 2 cut(s) 1391, 1527
AloI GAACNNNNNNTCC 2 cut(s) 2775, 2807
Alw21I GWGCWC 6 cut(s) 29, 35, 78, 892, 1214, 3310
Alw44I GTGCAC 4 cut(s) 74, 888, 1210, 3306
AlwI GGATC 8 cut(s) 1019, 2202, 2603, 2796, 3143, 3287, 3300, 4214
AlwNI CAGNNNCTG 1 cut(s) 401
Ama87I CYCGRG 2 cut(s) 28, 5290
Aor51HI AGCGCT 1 cut(s) 1670
AoxI GGCC 7 cut(s) 1049, 2568, 3096, 3830, 4230, 4312, 5369
ApaLI GTGCAC 4 cut(s) 74, 888, 1210, 3306
ArsI GACNNNNNNTTYG 4 cut(s) 4687, 4719, 4832, 4864
AseI ATTAAT 1 cut(s) 1406
AsiGI ACCGGT 1 cut(s) 4819
Asp700I GAANNNNTTC 8 cut(s) 321, 413, 975, 1407, 2203, 2412, 4122, 5196
AspLEI GCGC 7 cut(s) 91, 1019, 1358, 1671, 4164, 4672, 4721
AspS9I GGNCC 4 cut(s) 362, 2568, 3097, 3831
AsuC2I CCSGG 2 cut(s) 5097, 5115
AsuHPI GGTGA 4 cut(s) 131, 1451, 3022, 4108
AsuII TTCGAA 2 cut(s) 2794, 4293
AsuNHI GCTAGC 1 cut(s) 3265
AvaI CYCGRG 2 cut(s) 28, 5290
AvaII GGWCC 1 cut(s) 362
AxyI CCTNAGG 2 cut(s) 257, 2247
BaeGI GKGCMC 4 cut(s) 78, 892, 1214, 3310
BamHI GGATCC 1 cut(s) 3292
BanI GGYRCC 1 cut(s) 5079
BanII GRGCYC 4 cut(s) 29, 35, 4946, 5365
BarI GAAGNNNNNNTAC 2 cut(s) 2363, 2395
BauI CACGAG 1 cut(s) 1656
BbsI GAAGAC 1 cut(s) 4500
Bbv12I GWGCWC 6 cut(s) 29, 35, 78, 892, 1214, 3310
BceAI ACGGC 4 cut(s) 91, 1496, 3706, 4750
BcgI CGANNNNNNTGC 4 cut(s) 55, 89, 2808, 2842
BciT130I CCWGG 9 cut(s) 360, 991, 3233, 3599, 4304, 4991, 5003, 5084, 5131
BciVI GTATCC 2 cut(s) 1926, 5230
BclI TGATCA 3 cut(s) 136, 1494, 1966
BcnI CCSGG 2 cut(s) 5097, 5115
BcuI ACTAGT 1 cut(s) 1279
BfmI CTRYAG 6 cut(s) 352, 771, 1525, 2128, 3579, 4134
BfoI RGCGCY 2 cut(s) 1672, 4673
BfuAI ACCTGC 2 cut(s) 2965, 3202
BfuI GTATCC 2 cut(s) 1926, 5230
BmcAI AGTACT 1 cut(s) 350
Bme18I GGWCC 1 cut(s) 362
BmeT110I CYCGRG 2 cut(s) 28, 5290
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 4 cut(s) 362, 2568, 3097, 3831
BmiI GGNNCC 5 cut(s) 768, 2955, 3294, 4945, 5081
BmrI ACTGGG 2 cut(s) 156, 3699
BmtI GCTAGC 1 cut(s) 3269
BmuI ACTGGG 2 cut(s) 156, 3699
BpiI GAAGAC 1 cut(s) 4500
BplI GAGNNNNNCTC 6 cut(s) 2616, 2648, 4220, 4252, 5109, 5141
BpmI CTGGAG 8 cut(s) 914, 1001, 1796, 1808, 2485, 4062, 4247, 5113
Bpu10I CCTNAGC 2 cut(s) 1689, 3624
Bpu14I TTCGAA 2 cut(s) 2794, 4293
BpuEI CTTGAG 1 cut(s) 1214
BpuMI CCSGG 2 cut(s) 5097, 5115
BsaAI YACGTR 1 cut(s) 1850
BsaBI GATNNNNATC 1 cut(s) 2307
BsaHI GRCGYC 1 cut(s) 4784
BsaI GGTCTC 3 cut(s) 1180, 1438, 3663
BsaJI CCNNGG 4 cut(s) 1854, 2571, 4165, 4303
BsaWI WCCGGW 4 cut(s) 10, 1759, 4482, 4819
BsaXI ACNNNNNCTCC 6 cut(s) 2483, 2513, 2775, 2805, 5359, 5389
Bsc4I CCNNNNNNNGG 7 cut(s) 262, 371, 1613, 2735, 4921, 5113, 5297
Bse118I RCCGGY 4 cut(s) 115, 1429, 3310, 4819
Bse1I ACTGG 8 cut(s) 162, 853, 897, 2700, 3201, 3705, 3978, 4230
Bse21I CCTNAGG 2 cut(s) 257, 2247
Bse3DI GCAATG 4 cut(s) 1347, 2868, 3917, 4021
Bse8I GATNNNNATC 1 cut(s) 2307
BseBI CCWGG 9 cut(s) 360, 991, 3233, 3599, 4304, 4991, 5003, 5084, 5131
BseDI CCNNGG 4 cut(s) 1854, 2571, 4165, 4303
BseGI GGATG 9 cut(s) 271, 1012, 2356, 2725, 3057, 3625, 3778, 3915, 4177
BseJI GATNNNNATC 1 cut(s) 2307
BseLI CCNNNNNNNGG 7 cut(s) 262, 371, 1613, 2735, 4921, 5113, 5297
BseMI GCAATG 4 cut(s) 1347, 2868, 3917, 4021
BseMII CTCAG 9 cut(s) 672, 1391, 1703, 2007, 2238, 2754, 3638, 3757, 5167
BseNI ACTGG 8 cut(s) 162, 853, 897, 2700, 3201, 3705, 3978, 4230
BseRI GAGGAG 1 cut(s) 3061
BseSI GKGCMC 4 cut(s) 78, 892, 1214, 3310
BseYI CCCAGC 2 cut(s) 4664, 5284
BsgI GTGCAG 4 cut(s) 1172, 1830, 4558, 5039
Bsh1236I CGCG 2 cut(s) 89, 1358
BshFI GGCC 7 cut(s) 1051, 2570, 3098, 3832, 4232, 4314, 5371
BshNI GGYRCC 1 cut(s) 5079
BshTI ACCGGT 1 cut(s) 4819
BsiHKAI GWGCWC 6 cut(s) 29, 35, 78, 892, 1214, 3310
BsiHKCI CYCGRG 2 cut(s) 28, 5290
BslFI GGGAC 4 cut(s) 2440, 2496, 4541, 4816
BslI CCNNNNNNNGG 7 cut(s) 262, 371, 1613, 2735, 4921, 5113, 5297
BsmBI CGTCTC 1 cut(s) 1120
BsmFI GGGAC 4 cut(s) 2440, 2496, 4541, 4816
BsmI GAATGC 4 cut(s) 1822, 3278, 3502, 5227
BsnI GGCC 7 cut(s) 1051, 2570, 3098, 3832, 4232, 4314, 5371
Bso31I GGTCTC 3 cut(s) 1180, 1438, 3663
BsoBI CYCGRG 2 cut(s) 28, 5290
Bsp119I TTCGAA 2 cut(s) 2794, 4293
Bsp1286I GDGCHC 8 cut(s) 29, 35, 78, 892, 1214, 3310, 4946, 5365
Bsp1407I TGTACA 2 cut(s) 4174, 5058
Bsp19I CCATGG 1 cut(s) 1854
BspANI GGCC 7 cut(s) 1051, 2570, 3098, 3832, 4232, 4314, 5371
BspCNI CTCAG 9 cut(s) 673, 1390, 1702, 2006, 2239, 2755, 3637, 3756, 5166
BspFNI CGCG 2 cut(s) 89, 1358
BspHI TCATGA 1 cut(s) 1618
BspLI GGNNCC 5 cut(s) 768, 2955, 3294, 4945, 5081
BspMAI CTGCAG 3 cut(s) 775, 2132, 4138
BspMI ACCTGC 2 cut(s) 2965, 3202
BspOI GCTAGC 1 cut(s) 3269
BspPI GGATC 8 cut(s) 1019, 2202, 2603, 2796, 3143, 3287, 3300, 4214
BspQI GCTCTTC 2 cut(s) 1083, 1467
BspT104I TTCGAA 2 cut(s) 2794, 4293
BspT107I GGYRCC 1 cut(s) 5079
BspTNI GGTCTC 3 cut(s) 1180, 1438, 3663
BsrBI CCGCTC 1 cut(s) 2585
BsrDI GCAATG 4 cut(s) 1347, 2868, 3917, 4021
BsrFI RCCGGY 4 cut(s) 115, 1429, 3310, 4819
BsrGI TGTACA 2 cut(s) 4174, 5058
BsrI ACTGG 8 cut(s) 162, 853, 897, 2700, 3201, 3705, 3978, 4230
BssAI RCCGGY 4 cut(s) 115, 1429, 3310, 4819
BssECI CCNNGG 4 cut(s) 1854, 2571, 4165, 4303
BssNAI GTATAC 1 cut(s) 2909
BssNI GRCGYC 1 cut(s) 4784
BssSI CACGAG 1 cut(s) 1656
BssT1I CCWWGG 3 cut(s) 1854, 2571, 4165
Bst1107I GTATAC 1 cut(s) 2909
Bst2BI CACGAG 1 cut(s) 1656
Bst2UI CCWGG 9 cut(s) 360, 991, 3233, 3599, 4304, 4991, 5003, 5084, 5131
Bst6I CTCTTC 7 cut(s) 744, 954, 1083, 1118, 1467, 3348, 3889
BstACI GRCGYC 1 cut(s) 4784
BstAPI GCANNNNNTGC 4 cut(s) 203, 2678, 3537, 3919
BstAUI TGTACA 2 cut(s) 4174, 5058
BstBAI YACGTR 1 cut(s) 1850
BstBI TTCGAA 2 cut(s) 2794, 4293
BstDSI CCRYGG 1 cut(s) 1854
BstENI CCTNNNNNAGG 1 cut(s) 260
BstF5I GGATG 9 cut(s) 271, 1012, 2356, 2725, 3057, 3625, 3778, 3915, 4177
BstFNI CGCG 2 cut(s) 89, 1358
BstH2I RGCGCY 2 cut(s) 1672, 4673
BstHHI GCGC 7 cut(s) 91, 1019, 1358, 1671, 4164, 4672, 4721
BstNI CCWGG 9 cut(s) 360, 991, 3233, 3599, 4304, 4991, 5003, 5084, 5131
BstNSI RCATGY 6 cut(s) 1641, 1992, 3278, 3527, 3931, 4191
BstSFI CTRYAG 6 cut(s) 352, 771, 1525, 2128, 3579, 4134
BstSLI GKGCMC 4 cut(s) 78, 892, 1214, 3310
BstUI CGCG 2 cut(s) 89, 1358
BstV2I GAAGAC 1 cut(s) 4500
BstX2I RGATCY 2 cut(s) 3292, 4219
BstXI CCANNNNNNTGG 1 cut(s) 1820
BstYI RGATCY 2 cut(s) 3292, 4219
BstZ17I GTATAC 1 cut(s) 2909
Bsu36I CCTNAGG 2 cut(s) 257, 2247
BsuI GTATCC 2 cut(s) 1926, 5230
BsuRI GGCC 7 cut(s) 1051, 2570, 3098, 3832, 4232, 4314, 5371
BtgI CCRYGG 1 cut(s) 1854
BtgZI GCGATG 2 cut(s) 1367, 5346
BtrI CACGTC 1 cut(s) 241
BtsCI GGATG 9 cut(s) 271, 1012, 2356, 2725, 3057, 3625, 3778, 3915, 4177
BtsI GCAGTG 2 cut(s) 1497, 5259
BveI ACCTGC 2 cut(s) 2965, 3202
CaiI CAGNNNCTG 1 cut(s) 401
CciI TCATGA 1 cut(s) 1618
CfoI GCGC 7 cut(s) 91, 1019, 1358, 1671, 4164, 4672, 4721
Cfr10I RCCGGY 4 cut(s) 115, 1429, 3310, 4819
Cfr13I GGNCC 4 cut(s) 362, 2568, 3097, 3831
CseI GACGC 3 cut(s) 203, 1137, 4773
CsiI ACCWGGT 1 cut(s) 358
CspAI ACCGGT 1 cut(s) 4819
DraI TTTAAA 2 cut(s) 303, 3754
DraIII CACNNNGTG 1 cut(s) 3305
Eam1104I CTCTTC 7 cut(s) 744, 954, 1083, 1118, 1467, 3348, 3889
EarI CTCTTC 7 cut(s) 744, 954, 1083, 1118, 1467, 3348, 3889
EciI GGCGGA 1 cut(s) 5317
Ecl136II GAGCTC 2 cut(s) 27, 33
Eco130I CCWWGG 3 cut(s) 1854, 2571, 4165
Eco24I GRGCYC 4 cut(s) 29, 35, 4946, 5365
Eco31I GGTCTC 3 cut(s) 1180, 1438, 3663
Eco32I GATATC 1 cut(s) 5207
Eco47I GGWCC 1 cut(s) 362
Eco47III AGCGCT 1 cut(s) 1670
Eco53kI GAGCTC 2 cut(s) 27, 33
Eco81I CCTNAGG 2 cut(s) 257, 2247
Eco88I CYCGRG 2 cut(s) 28, 5290
EcoICRI GAGCTC 2 cut(s) 27, 33
EcoNI CCTNNNNNAGG 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 362
EcoRI GAATTC 3 cut(s) 975, 2551, 5213
EcoRII CCWGG 9 cut(s) 358, 989, 3231, 3597, 4302, 4989, 5001, 5082, 5129
EcoRV GATATC 1 cut(s) 5207
EcoT14I CCWWGG 3 cut(s) 1854, 2571, 4165
EcoT22I ATGCAT 2 cut(s) 1990, 3280
EcoT38I GRGCYC 4 cut(s) 29, 35, 4946, 5365
ErhI CCWWGG 3 cut(s) 1854, 2571, 4165
Esp3I CGTCTC 1 cut(s) 1120
FalI AAGNNNNNCTT 4 cut(s) 398, 430, 4668, 4700
FaqI GGGAC 4 cut(s) 2440, 2496, 4541, 4816
FauI CCCGC 5 cut(s) 119, 1777, 4027, 4291, 5171
FauNDI CATATG 2 cut(s) 283, 1390
FbaI TGATCA 3 cut(s) 136, 1494, 1966
FblI GTMKAC 2 cut(s) 2908, 5413
FokI GGATG 9 cut(s) 278, 1019, 2363, 2712, 3064, 3632, 3785, 3922, 4164
FriOI GRGCYC 4 cut(s) 29, 35, 4946, 5365
GlaI GCGC 7 cut(s) 90, 1018, 1357, 1670, 4163, 4671, 4720
GsaI CCCAGC 2 cut(s) 4668, 5288
GsuI CTGGAG 8 cut(s) 914, 1001, 1796, 1808, 2485, 4062, 4247, 5113
HaeII RGCGCY 2 cut(s) 1672, 4673
HaeIII GGCC 7 cut(s) 1051, 2570, 3098, 3832, 4232, 4314, 5371
HgaI GACGC 3 cut(s) 203, 1137, 4773
HhaI GCGC 7 cut(s) 91, 1019, 1358, 1671, 4164, 4672, 4721
Hin1I GRCGYC 1 cut(s) 4784
Hin6I GCGC 7 cut(s) 89, 1017, 1356, 1669, 4162, 4670, 4719
HinP1I GCGC 7 cut(s) 89, 1017, 1356, 1669, 4162, 4670, 4719
HincII GTYRAC 2 cut(s) 567, 5414
HindII GTYRAC 2 cut(s) 567, 5414
HindIII AAGCTT 7 cut(s) 440, 613, 752, 1291, 1695, 2539, 4466
HphI GGTGA 4 cut(s) 131, 1451, 3022, 4108
Hpy99I CGWCG 4 cut(s) 112, 158, 242, 5345
HpyCH4IV ACGT 5 cut(s) 240, 1849, 3613, 4935, 4977
HpySE526I ACGT 5 cut(s) 240, 1849, 3613, 4935, 4977
Hsp92I GRCGYC 1 cut(s) 4784
HspAI GCGC 7 cut(s) 89, 1017, 1356, 1669, 4162, 4670, 4719
Ksp22I TGATCA 3 cut(s) 136, 1494, 1966
LguI GCTCTTC 2 cut(s) 1083, 1467
LmnI GCTCC 3 cut(s) 38, 1783, 4949
MabI ACCWGGT 1 cut(s) 358
MaeII ACGT 5 cut(s) 240, 1849, 3613, 4935, 4977
MbiI CCGCTC 1 cut(s) 2585
MfeI CAATTG 3 cut(s) 1878, 3723, 4512
MflI RGATCY 2 cut(s) 3292, 4219
MhlI GDGCHC 8 cut(s) 29, 35, 78, 892, 1214, 3310, 4946, 5365
MlyI GAGTC 4 cut(s) 570, 905, 1648, 3370
MmeI TCCRAC 8 cut(s) 146, 553, 1631, 2281, 3320, 3738, 3825, 4971
Mph1103I ATGCAT 2 cut(s) 1990, 3280
MroXI GAANNNNTTC 8 cut(s) 321, 413, 975, 1407, 2203, 2412, 4122, 5196
MslI CAYNNNNRTG 5 cut(s) 1391, 1527, 2075, 2730, 3999
MspA1I CMGCKG 6 cut(s) 200, 2297, 2645, 3171, 4757, 5419
MunI CAATTG 3 cut(s) 1878, 3723, 4512
Mva1269I GAATGC 4 cut(s) 1822, 3278, 3502, 5227
MvaI CCWGG 9 cut(s) 360, 991, 3233, 3599, 4304, 4991, 5003, 5084, 5131
MvnI CGCG 2 cut(s) 89, 1358
NciI CCSGG 2 cut(s) 5097, 5115
NcoI CCATGG 1 cut(s) 1854
NdeI CATATG 2 cut(s) 283, 1390
NheI GCTAGC 1 cut(s) 3265
NlaIV GGNNCC 5 cut(s) 768, 2955, 3294, 4945, 5081
NmeAIII GCCGAG 3 cut(s) 20, 1851, 5397
NmuCI GTSAC 6 cut(s) 1643, 1919, 1952, 3010, 3073, 3337
NsiI ATGCAT 2 cut(s) 1990, 3280
NspI RCATGY 6 cut(s) 1641, 1992, 3278, 3527, 3931, 4191
NspV TTCGAA 2 cut(s) 2794, 4293
OliI CACNNNNGTG 2 cut(s) 1391, 1527
PaeI GCATGC 3 cut(s) 1992, 3278, 3527
PaeR7I CTCGAG 1 cut(s) 28
PagI TCATGA 1 cut(s) 1618
PciI ACATGT 2 cut(s) 3927, 4187
PciSI GCTCTTC 2 cut(s) 1083, 1467
PctI GAATGC 4 cut(s) 1822, 3278, 3502, 5227
PdmI GAANNNNTTC 8 cut(s) 321, 413, 975, 1407, 2203, 2412, 4122, 5196
PflFI GACNNNGTC 1 cut(s) 360
PfoI TCCNGGA 1 cut(s) 3231
PinAI ACCGGT 1 cut(s) 4819
PleI GAGTC 4 cut(s) 570, 904, 1648, 3369
PpsI GAGTC 4 cut(s) 570, 904, 1648, 3369
Ppu21I YACGTR 1 cut(s) 1850
PpuMI RGGWCCY 1 cut(s) 362
PscI ACATGT 2 cut(s) 3927, 4187
PshBI ATTAAT 1 cut(s) 1406
PsiI TTATAA 1 cut(s) 3413
Psp124BI GAGCTC 2 cut(s) 29, 35
Psp1406I AACGTT 2 cut(s) 3613, 4935
Psp5II RGGWCCY 1 cut(s) 362
Psp6I CCWGG 9 cut(s) 358, 989, 3231, 3597, 4302, 4989, 5001, 5082, 5129
PspFI CCCAGC 2 cut(s) 4664, 5284
PspGI CCWGG 9 cut(s) 358, 989, 3231, 3597, 4302, 4989, 5001, 5082, 5129
PspN4I GGNNCC 5 cut(s) 768, 2955, 3294, 4945, 5081
PspPI GGNCC 4 cut(s) 362, 2568, 3097, 3831
PspPPI RGGWCCY 1 cut(s) 362
PspXI VCTCGAGB 1 cut(s) 28
PsrI GAACNNNNNNTAC 2 cut(s) 3774, 3806
PstI CTGCAG 3 cut(s) 775, 2132, 4138
PstNI CAGNNNCTG 1 cut(s) 401
PsuI RGATCY 2 cut(s) 3292, 4219
PsyI GACNNNGTC 1 cut(s) 360
PvuII CAGCTG 4 cut(s) 200, 2297, 2645, 4757
RseI CAYNNNNRTG 5 cut(s) 1391, 1527, 2075, 2730, 3999
SacI GAGCTC 2 cut(s) 29, 35
SalI GTCGAC 1 cut(s) 5412
SapI GCTCTTC 2 cut(s) 1083, 1467
Sau96I GGNCC 4 cut(s) 362, 2568, 3097, 3831
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 4 cut(s) 570, 905, 1648, 3370
SduI GDGCHC 8 cut(s) 29, 35, 78, 892, 1214, 3310, 4946, 5365
SexAI ACCWGGT 1 cut(s) 358
SfcI CTRYAG 6 cut(s) 352, 771, 1525, 2128, 3579, 4134
Sfr274I CTCGAG 1 cut(s) 28
SfuI TTCGAA 2 cut(s) 2794, 4293
SinI GGWCC 1 cut(s) 362
SlaI CTCGAG 1 cut(s) 28
SmiMI CAYNNNNRTG 5 cut(s) 1391, 1527, 2075, 2730, 3999
SmlI CTYRAG 2 cut(s) 28, 1229
SmoI CTYRAG 2 cut(s) 28, 1229
SpeI ACTAGT 1 cut(s) 1279
SphI GCATGC 3 cut(s) 1992, 3278, 3527
SspI AATATT 2 cut(s) 1195, 5380
SstI GAGCTC 2 cut(s) 29, 35
StyI CCWWGG 3 cut(s) 1854, 2571, 4165
TaiI ACGT 5 cut(s) 243, 1852, 3616, 4938, 4980
TaqII GACCGA 1 cut(s) 3783
TauI GCSGC 4 cut(s) 4014, 4302, 4621, 5009
TseFI GTSAC 6 cut(s) 1643, 1919, 1952, 3010, 3073, 3337
Tsp45I GTSAC 6 cut(s) 1643, 1919, 1952, 3010, 3073, 3337
TspGWI ACGGA 4 cut(s) 63, 451, 3246, 3674
Tth111I GACNNNGTC 1 cut(s) 360
VneI GTGCAC 4 cut(s) 74, 888, 1210, 3306
VpaK11BI GGWCC 1 cut(s) 362
VspI ATTAAT 1 cut(s) 1406
XagI CCTNNNNNAGG 1 cut(s) 260
XbaI TCTAGA 1 cut(s) 521
XceI RCATGY 6 cut(s) 1641, 1992, 3278, 3527, 3931, 4191
XcmI CCANNNNNNNNNTGG 1 cut(s) 2103
XhoI CTCGAG 1 cut(s) 28
XmiI GTMKAC 2 cut(s) 2908, 5413
XmnI GAANNNNTTC 8 cut(s) 321, 413, 975, 1407, 2203, 2412, 4122, 5196
ZrmI AGTACT 1 cut(s) 350
Zsp2I ATGCAT 2 cut(s) 1990, 3280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.