Rroxscaffold_2G00085180

Sister chromatid cohesion protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010273
Physical Location & Seq
Reverse (-)
7525859 .. 7540403
14545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00085180.1

Sequence Viewer

Length: 5460 bp
ATGGATGATTTGCAGGTAATGCTGGGCACTTTGAAGAGAATGAATGATTCTGAGATTTTACCTTGCTATCCAGTTTTACCAAATACACTGCAATTTTGTGGTTTTGATAAGTGTGATCATAAGTATGAGCTCCTGAACTACACAGTGAGGTGCTCATTCTTGTTTAGGAAGCCTGGAGAGAAGAACAGTGATCAGCTATCAGATGCAAGCAGTTATCTCATTTGCGTAGATGTGTGCTATTTGTTCTTAGCAGCAGATACTGCGGCGGTTCAATATGGCTATGTCCAACCTATGGAACTTCACGATGAAGTTTTACAGTACAATCCAGAAGCCTTTCAGTATACTACTCCAGGTCCTATTATGGGGCAAGTGCCTGGCAGTAGTACAGTACCAGATAAGAAGCCCTTTGTGCCAATTATACCTATAACAAGAACTTCTGAAAGAGATTATGGTGCAACTCACAATAGAGATTATGGTGCAACTCACAATAATCAGCTCAACTATACTCCTCCCAATAACATATCTATACCATCCTCCAGAAAACCAAAAGCAAAGAGAAAAGCTAGTGACGGCGTCACTTCCTCAGTTGGGTCAGATCCCGTTGCAAGTCAAGAAGCCACTGTTGGATGCTTTTGTGAGTTGGTGGAGGATTTTTGCGGCAGAGCTGAAATTTTCAGTGAGGATCGGGACGAAGCACAGTGGTTATCTATGCCTCTTTCTGATCTTAGAATGCTTGCAAATGAAATAATGTCTCTTCGTGCAAAGAGGCTTCTACATATGGTTCCTGTAGATACTTTTGTTAGATTGTTAAGGATTTTAGATCATCAGATACATGGAGCTGAAGGCTTGTCTATAAATGAACAGTCAGATTCAAATGGGGTTTCATCAATCAATTGTGCACTAGAGTCCATTCATGCAGCTTTAGCAGTAATGGCCCATAGCCAGATGCCAAAGCAGCTGTATAAAGAAGAGATCATTGAAAGAATTCTGGAGTTCTCCAGGCATCAGATAATGGATGTTATGTGTGCTTGCGATCCATCATATCGTGCTTTACATAGGCCAAGTGAAAATGGGACACTTGAAGTTGAGGAAGACGAAATTCTTGATGCTGAAACTGGTTCAGCAAGCAAGAGGCGACGTAGTACTAAGACTGTTAAAGTGCAGAAATCATCATATAACAGGCGGTTTCACCTTTCCTTACGTCCTTTTCTTGGTAGGATCTCTGCTGCTGTGAATAATATTCTTCAGAAAATGTGCACAATTCTTGGTTTGCTAAAGGATTTGTTGCTGATAGAAAAGTTGTCAGATAGTTGTATCTTACAACTACTGAAAACAAGCTTTACAACATTTATGGTGGATAATATCCAGCTTTTGCAACTGAAGGCAATTGGTTTGATCAGTGGGATATACTATTCATACACTCAACATCGGACTTATGTTATAGATGAGTTACTTCAGCTGCTTTGGAAGTTGCCTTTCTCGAAGCGAGCTTTGAGAGCCTATCACCTACCTGATGAAGAGCAAACGCAGATTCAAATGATCACTGCTTTGCTGATTCAGTTGGTTCACTGTAGTGCAAACCTTCCTGCACCTTTAAGGCAGGATTCAAGTGTTAATTCCATCTTAGAAGTTTCTGACAATGCTGATTATCCAATTAAAGGCCTTGAGGCAGCACAAGCAGCGTGTTGTCACTTTTGGAAAGGGGTACTTCAGCGGTTTGCTAACGTCAAGAACCAGGAAGCTTCAGAATTCAAAGTTATGATGGAGAATCTTGTTACCGATCTGCTGACAACATTGAATTTACCTGAATATCCCGCTTCAGCCCCTATTCTGGAGGTTCTTTGTGTCTTGTTACTCGCGAATGCTGGGGTGAAATCTAAAGATGTAGCTGCACGTAGCATGGCTATCGACCTTCTTGGAACCATTGCTGCGAGGTTGAAGCGTGATTCTCTCCTTCGCAGTAGGGGCAAATTTTGGATATTACAGGAGTTGGTTAGTGGGGATGCGGCTGATCAGACTTATCCAAAAGATGTATGCTCTGCTTGTTTAGATGGCAGGGTTGAAAAGACTTTCTTTGTATGTCAAGGTTGTCAGAGAATGTTTCACGCGGATTGCATGGGAGTAAGAGAGCATGAAGTTCCTAACCAATCTTGGCACTGTCAGATATGCCTCTGCCGGAAACAACTTCTTGTTTTACAATCTTACTGCAAGTCACAATACAAAGATGATGGATTTAAGGATCATAAAAATTCAGGAAGAAAAACTGAAGTTACTTTTTCAATTACTAAACTTGAGATCGTCCAACAGTTGCTTTTGAATTACCTACAAGATACCGCATCAGCTGATGATGTTCATCTCTTTGTTCGTTGGCTTTATGTCTGCTTGTGGTACAAGGATGATCCACCAAAGTCCCAGCAGAAGTTCATTTACTATCTTGCAAGACTGAACTCAAAAGCAATAGTGCGGGATTCTGGGACTGTTTTTTCCTTGCTGACAAGGGACTTAATCAAGCAGATTACCTTAGTATTGGGACGAAATACTTCTTTTGCCAGAGGATTTGATAAGATTCTACACCTGCTTCTGGCAAGTCTAAGGGAGAATTCTCCTGTAATCAGGGCCAAGGCTTTACGAGCGGTTAGTATAGTTGTAGAGGCTGATCCGGAGGTTTTAGGTGACAAACGTGTGCAACCAGCTGTTGAAGGAAGGTTTTGTGATTCCGCAATTTCTGTTAGAGAAGCAGCACTGGAACTCGTAGGGAGGCATATTGCTTCCCATCCTGATGTTGGTTTGAAGTATTTTGAGAAGGTTGCTGAGAGAATCAAAGATACGGGTGTGAGTGTTCGAAAGCGATCTATTAAAATCATACGAGATATGTGCGTTTCAAATGGGGACTTCTCTGAATTTACTAGTGCTTGCATTGCTATCATCTCCCGTATTGGTGACGACGAATCAAGTATACAGGATCTTGTTTGCAAGACATTTTATGAGTTCTGGTTTGAGGAACATACTGGTTCACATACTCAATTCTTTGGAGATGATAGCTCTGTTCCATTGGAGGTGGCTAAAAAAACTGAACAGATTGTTGAGATGTTGAGGAGGATGCCAACACCACACCATCTTGTTACCGTCATTAAACGCAACTTAGCCCTTGATTTTTTCCCACAAGCAGCTAAAGCTGCGGGAATCAATCCCGTCTTACTTGCATCAGTACGCAATCGGTGTGAGCTAATGTGCAAGTTTTTACTGGAAAGGATATTGCAGGTAGAAGAAATGAATATCCAGGAAGTTGAGATGCGTGCTCTTCCATATGTGCAAGTTCTGCATGCATTTTGTGTTGTGGATCCAACATTATGTGCACCGGTTTCTAACCCTTCGCAGTTTGTGGTCACTCTTCAGCCTTATCTAAAGAGTCAGGATGATAATCGAGTGGTTGCCAAGTTATTGGAGAGCATAATATTCATAATTGATTCTGTCCTCCCTTTAGTAAGAAAGTTGCCACAAAATGTTCTTGAAGAACTTGAACAAGACTTAAAGAGCATGATTGTTCGGCATTCCTTCTTGACAGTTGTTCATGCTTGCATCAAGTGCCTCTGTGCTGTGAGTAAAGTGGCAGGAAAAGGCGCCGCTGTTGTGGAGTATCTAATTCAGGTTTTTTACAAACGGCTGGATGCTGAGGAAGTTGACAACCAGCAGAAAGCGGGGCGATCTCTTTTCTGTCTTGGAATGCTTATCCGTTATGGCAACTCTTTACTGTGTTACTCTGACCAAACAATTGATGTTGCAAGCAGTCTCGGCTTATTTAAAAGATATCTTCTAATGGACGATTTTTTCTTAAAGGCTAGATCATTGCAGGCATTGGGCTTTGTGTTAATTGCTAGGCCTGAATTTATGTTGGAAAAAGATATTGGGAAGATATTAGAGGAGACATTCTCTTCTAGCTCTGATGTCCGTCTCAAGATGCAAGCTTTGCAAAACATGTACGACTATCTTCTTGATGCTGAAACTCAATTGGGAACGGATACAACCAGTAATAATGTGGCTGATTATTCTGTGGAGGGTGGCAATGCTGTACCTGTTGCTGCGGGTGCTGGAGATACTAATATATGTGGCGGAATAGTTCAGTTGTATTGGGATAATATTCTGGAGAGATGTTTGGACTTTAATGAACAAATTCGCAGCTCTGCCCTTAAGATTGTTGAAGTTGTGCTGCGCCAAGGGCTTGTGCATCCTATTACTTGTGTTCCATTCCTTATTGCACTGGAAACAGATCCACTGGAGGCCAATTCGACATTAGCGCATCATTTGCTGATGAATATGAATGAAAAGTACCCTTCATTTTTTGAAAGCCGACTAGGGGATGGCCTTCAAATGTCTTTTGGATTTATACAATCCATACGTCCTGGTACTGAACGTGAGAACACAAAACCACCATCAAAGGCTTCTGGAAATGCAAAGGGAAAAGTTGATGATGTTTCTTTTGCTCAAGCAAGATTGGGAGTCTCTCGAATCTACAAGCTTATTCGTGCTAACCGTGTTTCAAGGAACAAATTTATGTCCTCAATTGTTCGCAAGTTTGATAATCCTAGCTGGACCACTTCAGTAGTCCCTTTCCTCATGTACTGCACGGAAATACTTGCCTTGCTGCCATTCACAACACCTGATGAGCCCCTTTATTTAGTCTATGCTATAAATAGAGTAATACAAGTTAAGGCTGGGCAACTTGAAGCAAAGCTGAAAGCTCTGACATTGCATCTGTTACAAAGAGGTGCAACCCGTGGGAATGGCATAATTAAAGAGGACCTGGCTGCTCCGCCCTTTACAAGAGGAATGGCATCAGTGGATTTGAATGGGACAATTGAACCAGAACCAGCTTATTACATGGCAGCTATGGATTTGAATGGGACAATTGAACAAGACCCAGCTGATCAGTCTGTCTCAAATCAAGATACTGTGTTAGAGGCAAAGATGCACGGTAAAGGTTCTGTCAGTTCCTCTGGCATCTCAATAGATGATGTACAGATAATCCAGGCAGACTGCCTGGCAGCTATTGCATTGCAGCTTCTTTTGAAACTGAAGAGACACCTAAAAATTGTGTATAGCTTGAATGATGCTAGGTGCCAGGCTTTCTCTCCAACCGATCCCATTAAACCTGGAGATGCTTTCTCCAAGCAGAGCATTCCTTTTGATGTCAGTGACACACATACTAACTTGCCTGGAACTTATCAAGAATTGGTACAAAGATATCAGGAATTCAAGAATGCATTGAAGGATGATACAGTTGATTTCTCAACTTACACAGCAAATCTCAAAAGAAAACGTCCGGCCCCTAGGAAAGGAAGGAAATCTGGAGTTAGAGATGATGAGGATGACAATGATGATGATGAAGATTGGTCAGGTGGGGCTCGGAGGCTGAGTTATAGTGGGAGGAGAGGTGGCCACAGTAGAAGTCGACAACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000070 GO:0000122 GO:0000278 GO:0000280 GO:0000428 GO:0000819 GO:0001654 GO:0001655 GO:0001656 GO:0001822 GO:0003006 GO:0003007 GO:0003008 GO:0003151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007064 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0007423 GO:0007507 GO:0007548 GO:0007600 GO:0007605 GO:0008022 GO:0008104 GO:0008150 GO:0009314 GO:0009628 GO:0009653 GO:0009790 GO:0009887 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010165 GO:0010171 GO:0010212 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010720 GO:0016043 GO:0016591 GO:0019219 GO:0019222 GO:0019899 GO:0019904 GO:0022008 GO:0022402 GO:0022414 GO:0030154 GO:0030326 GO:0030334 GO:0030335 GO:0030880 GO:0031056 GO:0031058 GO:0031063 GO:0031065 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032039 GO:0032116 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0033365 GO:0033554 GO:0034085 GO:0034086 GO:0034088 GO:0034502 GO:0034613 GO:0035107 GO:0035108 GO:0035112 GO:0035113 GO:0035115 GO:0035136 GO:0035239 GO:0035261 GO:0035295 GO:0040007 GO:0040008 GO:0040012 GO:0040017 GO:0042471 GO:0042634 GO:0042826 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043388 GO:0043583 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045597 GO:0045892 GO:0045934 GO:0045995 GO:0047485 GO:0048285 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048546 GO:0048557 GO:0048562 GO:0048565 GO:0048566 GO:0048568 GO:0048589 GO:0048592 GO:0048598 GO:0048608 GO:0048638 GO:0048699 GO:0048731 GO:0048736 GO:0048806 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0050896 GO:0050954 GO:0051094 GO:0051098 GO:0051099 GO:0051101 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051253 GO:0051270 GO:0051272 GO:0051276 GO:0051641 GO:0051716 GO:0051960 GO:0051962 GO:0055029 GO:0055123 GO:0060065 GO:0060173 GO:0060255 GO:0060284 GO:0060322 GO:0060323 GO:0060324 GO:0060325 GO:0061008 GO:0061010 GO:0061038 GO:0061458 GO:0061695 GO:0065007 GO:0065009 GO:0070013 GO:0070087 GO:0070727 GO:0071168 GO:0071214 GO:0071478 GO:0071479 GO:0071481 GO:0071840 GO:0071921 GO:0072001 GO:0072359 GO:0080090 GO:0090311 GO:0090312 GO:0090596 GO:0090694 GO:0098813 GO:0104004 GO:0140014 GO:1902275 GO:1902494 GO:1902679 GO:1903047 GO:1903506 GO:1903507 GO:1905269 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2000145 GO:2000147 GO:2001141 GO:2001222 GO:2001224 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04111 map04111
Pfam Domains
Protein Families
Cohesin_HEAT Nipped-B_C PHD

Protein Analysis

1819

Amino Acids

203.99

Weight (kDa)

6.5

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cohesin_HEAT PF12765 877 - 918 1.2e-12 HEAT repeat associated with sister chromatid cohesion
Nipped-B_C PF12830 1369 - 1566 1.2e-51 Sister chromatid cohesion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15540 AT5G15540
fragaria_vesca FvH4_6g47080 FvH4_6g47080 FvH4_6g47080
malus_domestica MD09G1064300.v1.1 MD17G1057600.v1.1
prunus_persica Prupe.3G256700_v2.0.a1
pyrus_communis pycom111g05400 pycom17g05770
rosa_chinensis RchiOBHm_Chr2g0166131
rosa_laevigata RLG00000021588
rosa_multiflora Rmu_sc0006218.1_g000028
rosa_roxburghii Rroxscaffold_2G00085180
rosa_rugosa Rorug02G0520500 Rorug02G0520600
rosa_samantha Rh2AG586600 Rh2BG598400 Rh2CG569100 Rh2DG608300
rosa_wichuraiana Rw2G048900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2583
Acc36I ACCTGC 3 cut(s) 4, 2583, 3250
AccB1I GGYRCC 2 cut(s) 3620, 5115
AccB7I CCANNNNNTGG 1 cut(s) 292
AccBSI CCGCTC 1 cut(s) 2633
AccI GTMKAC 3 cut(s) 341, 2956, 5449
AccII CGCG 2 cut(s) 1859, 2108
AccIII TCCGGA 1 cut(s) 2659
AcoI YGGCCR 1 cut(s) 5434
AcyI GRCGYC 2 cut(s) 573, 3621
AflII CTTAAG 1 cut(s) 4187
AflIII ACRYGT 2 cut(s) 2680, 3975
AgeI ACCGGT 1 cut(s) 3358
AhlI ACTAGT 1 cut(s) 2906
AjuI GAANNNNNNNTTGG 4 cut(s) 606, 638, 3888, 3920
AleI CACNNNNGTG 1 cut(s) 1572
Alw21I GWGCWC 6 cut(s) 132, 155, 901, 1259, 3301, 3358
Alw26I GTCTC 7 cut(s) 756, 3794, 3917, 3956, 4504, 4939, 5071
Alw44I GTGCAC 3 cut(s) 897, 1255, 3354
AlwNI CAGNNNCTG 1 cut(s) 260
Aor13HI TCCGGA 1 cut(s) 2659
AoxI GGCC 9 cut(s) 933, 1058, 1660, 2616, 3878, 4278, 4360, 5322, 5434
ApaLI GTGCAC 3 cut(s) 897, 1255, 3354
AsiGI ACCGGT 1 cut(s) 3358
Asp700I GAANNNNTTC 4 cut(s) 333, 984, 2539, 4170
AspA2I CCTAGG 1 cut(s) 5327
AspLEI GCGC 3 cut(s) 3623, 4212, 4297
AspS9I GGNCC 6 cut(s) 353, 934, 2616, 4590, 4798, 5323
AsuHPI GGTGA 5 cut(s) 1181, 1496, 1882, 2684, 2951
AsuII TTCGAA 1 cut(s) 2842
AvaII GGWCC 3 cut(s) 353, 4590, 4798
AvrII CCTAGG 1 cut(s) 5327
BaeGI GKGCMC 4 cut(s) 29, 901, 1259, 3358
BaeI ACNNNNGTAYC 4 cut(s) 783, 816, 2817, 2850
BalI TGGCCA 1 cut(s) 5436
BamHI GGATCC 1 cut(s) 3340
BanI GGYRCC 2 cut(s) 3620, 5115
BanII GRGCYC 3 cut(s) 132, 4668, 5404
BarI GAAGNNNNNNTAC 2 cut(s) 2411, 2443
BbsI GAAGAC 1 cut(s) 1098
Bbv12I GWGCWC 6 cut(s) 132, 155, 901, 1259, 3301, 3358
BbvCI CCTCAGC 1 cut(s) 3672
BceAI ACGGC 2 cut(s) 586, 3677
BcgI CGANNNNNNTGC 4 cut(s) 1888, 1922, 2856, 2890
BciVI GTATCC 1 cut(s) 4012
BclI TGATCA 6 cut(s) 115, 190, 1395, 1539, 2011, 4924
BcoDI GTCTC 7 cut(s) 756, 3794, 3917, 3956, 4504, 4939, 5071
BcuI ACTAGT 1 cut(s) 2906
BfmI CTRYAG 2 cut(s) 786, 1570
BfoI RGCGCY 1 cut(s) 3624
BfrI CTTAAG 1 cut(s) 4187
BfuAI ACCTGC 3 cut(s) 4, 2583, 3250
BfuI GTATCC 1 cut(s) 4012
BlnI CCTAGG 1 cut(s) 5327
BmcAI AGTACT 1 cut(s) 1144
Bme18I GGWCC 3 cut(s) 353, 4590, 4798
BmgT120I GGNCC 6 cut(s) 353, 934, 2616, 4590, 4798, 5323
BmiI GGNNCC 6 cut(s) 783, 1921, 3342, 3622, 5117, 5325
BpiI GAAGAC 1 cut(s) 1098
BplI GAGNNNNNCTC 2 cut(s) 3914, 3946
Bpu10I CCTNAGC 1 cut(s) 3672
Bpu14I TTCGAA 1 cut(s) 2842
BpuEI CTTGAG 4 cut(s) 1685, 2312, 3938, 4467
BsaAI YACGTR 1 cut(s) 1895
BsaBI GATNNNNATC 2 cut(s) 2352, 3420
BsaHI GRCGYC 2 cut(s) 573, 3621
BsaJI CCNNGG 4 cut(s) 2619, 4213, 4774, 5327
BsaWI WCCGGW 2 cut(s) 2659, 3358
BsaXI ACNNNNNCTCC 4 cut(s) 5398, 5416, 5428, 5446
Bse118I RCCGGY 1 cut(s) 3358
Bse1I ACTGG 8 cut(s) 71, 1120, 2748, 3013, 3249, 4026, 4263, 4278
Bse3DI GCAATG 6 cut(s) 1923, 2916, 3845, 4069, 4745, 5051
Bse8I GATNNNNATC 2 cut(s) 2352, 3420
BseAI TCCGGA 1 cut(s) 2659
BseDI CCNNGG 4 cut(s) 2619, 4213, 4774, 5327
BseJI GATNNNNATC 2 cut(s) 2352, 3420
BseMI GCAATG 6 cut(s) 1923, 2916, 3845, 4069, 4745, 5051
BseMII CTCAG 5 cut(s) 42, 597, 2802, 3663, 5402
BseNI ACTGG 8 cut(s) 71, 1120, 2748, 3013, 3249, 4026, 4263, 4278
BseRI GAGGAG 4 cut(s) 498, 3109, 3935, 5440
BseSI GKGCMC 4 cut(s) 29, 901, 1259, 3358
BseYI CCCAGC 5 cut(s) 22, 1865, 2412, 4712, 4918
BsgI GTGCAG 4 cut(s) 1181, 1572, 1875, 4606
Bsh1236I CGCG 2 cut(s) 1859, 2108
BshFI GGCC 9 cut(s) 935, 1060, 1662, 2618, 3880, 4280, 4362, 5324, 5436
BshNI GGYRCC 2 cut(s) 3620, 5115
BshTI ACCGGT 1 cut(s) 3358
BsiHKAI GWGCWC 6 cut(s) 132, 155, 901, 1259, 3301, 3358
BsiSI CCGG 4 cut(s) 2176, 2660, 3359, 5321
BsmAI GTCTC 7 cut(s) 756, 3794, 3917, 3956, 4504, 4939, 5071
BsmBI CGTCTC 1 cut(s) 3956
BsmI GAATGC 6 cut(s) 735, 1867, 3550, 3729, 5175, 5263
BsnI GGCC 9 cut(s) 935, 1060, 1662, 2618, 3880, 4280, 4362, 5324, 5436
Bsp119I TTCGAA 1 cut(s) 2842
Bsp1286I GDGCHC 9 cut(s) 29, 132, 155, 901, 1259, 3301, 3358, 4668, 5404
Bsp13I TCCGGA 1 cut(s) 2659
Bsp1407I TGTACA 1 cut(s) 5014
Bsp68I TCGCGA 1 cut(s) 1859
BspANI GGCC 9 cut(s) 935, 1060, 1662, 2618, 3880, 4280, 4362, 5324, 5436
BspCNI CTCAG 5 cut(s) 43, 596, 2803, 3664, 5403
BspEI TCCGGA 1 cut(s) 2659
BspFNI CGCG 2 cut(s) 1859, 2108
BspLI GGNNCC 6 cut(s) 783, 1921, 3342, 3622, 5117, 5325
BspMI ACCTGC 3 cut(s) 4, 2583, 3250
BspQI GCTCTTC 2 cut(s) 1512, 3306
BspT104I TTCGAA 1 cut(s) 2842
BspT107I GGYRCC 2 cut(s) 3620, 5115
BspTI CTTAAG 1 cut(s) 4187
BsrBI CCGCTC 1 cut(s) 2633
BsrDI GCAATG 6 cut(s) 1923, 2916, 3845, 4069, 4745, 5051
BsrFI RCCGGY 1 cut(s) 3358
BsrGI TGTACA 1 cut(s) 5014
BsrI ACTGG 8 cut(s) 71, 1120, 2748, 3013, 3249, 4026, 4263, 4278
BssAI RCCGGY 1 cut(s) 3358
BssECI CCNNGG 4 cut(s) 2619, 4213, 4774, 5327
BssNAI GTATAC 2 cut(s) 342, 2957
BssNI GRCGYC 2 cut(s) 573, 3621
BssT1I CCWWGG 3 cut(s) 2619, 4213, 5327
Bst1107I GTATAC 2 cut(s) 342, 2957
Bst6I CTCTTC 8 cut(s) 29, 759, 963, 1512, 3306, 3396, 3937, 5069
BstACI GRCGYC 2 cut(s) 573, 3621
BstAFI CTTAAG 1 cut(s) 4187
BstAPI GCANNNNNTGC 7 cut(s) 19, 260, 3319, 3585, 3967, 4303, 5048
BstAUI TGTACA 1 cut(s) 5014
BstBAI YACGTR 1 cut(s) 1895
BstBI TTCGAA 1 cut(s) 2842
BstDSI CCRYGG 1 cut(s) 4774
BstENI CCTNNNNNAGG 1 cut(s) 5331
BstFNI CGCG 2 cut(s) 1859, 2108
BstH2I RGCGCY 1 cut(s) 3624
BstHHI GCGC 3 cut(s) 3623, 4212, 4297
BstMAI GTCTC 7 cut(s) 756, 3794, 3917, 3956, 4504, 4939, 5071
BstNSI RCATGY 2 cut(s) 3326, 3979
BstSFI CTRYAG 2 cut(s) 786, 1570
BstSLI GKGCMC 4 cut(s) 29, 901, 1259, 3358
BstUI CGCG 2 cut(s) 1859, 2108
BstV2I GAAGAC 1 cut(s) 1098
BstX2I RGATCY 5 cut(s) 595, 1218, 2962, 3340, 4267
BstXI CCANNNNNNTGG 1 cut(s) 3442
BstYI RGATCY 5 cut(s) 595, 1218, 2962, 3340, 4267
BstZ17I GTATAC 2 cut(s) 342, 2957
BsuI GTATCC 1 cut(s) 4012
BsuRI GGCC 9 cut(s) 935, 1060, 1662, 2618, 3880, 4280, 4362, 5324, 5436
BtgI CCRYGG 1 cut(s) 4774
BtsI GCAGTG 2 cut(s) 86, 1542
BtuMI TCGCGA 1 cut(s) 1859
BveI ACCTGC 3 cut(s) 4, 2583, 3250
CaiI CAGNNNCTG 1 cut(s) 260
CfoI GCGC 3 cut(s) 3623, 4212, 4297
Cfr10I RCCGGY 1 cut(s) 3358
Cfr13I GGNCC 6 cut(s) 353, 934, 2616, 4590, 4798, 5323
CseI GACGC 1 cut(s) 562
CspAI ACCGGT 1 cut(s) 3358
DinI GGCGCC 1 cut(s) 3622
DraI TTTAAA 1 cut(s) 3802
EaeI YGGCCR 1 cut(s) 5434
Eam1104I CTCTTC 8 cut(s) 29, 759, 963, 1512, 3306, 3396, 3937, 5069
EarI CTCTTC 8 cut(s) 29, 759, 963, 1512, 3306, 3396, 3937, 5069
EciI GGCGGA 2 cut(s) 4125, 4800
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 3 cut(s) 2619, 4213, 5327
Eco147I AGGCCT 2 cut(s) 1662, 3880
Eco24I GRGCYC 3 cut(s) 132, 4668, 5404
Eco32I GATATC 2 cut(s) 3809, 5243
Eco47I GGWCC 3 cut(s) 353, 4590, 4798
Eco53kI GAGCTC 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 130
EcoNI CCTNNNNNAGG 1 cut(s) 5331
EcoO109I RGGNCCY 2 cut(s) 353, 4798
EcoRI GAATTC 4 cut(s) 984, 1748, 2599, 5249
EcoRV GATATC 2 cut(s) 3809, 5243
EcoT14I CCWWGG 3 cut(s) 2619, 4213, 5327
EcoT22I ATGCAT 2 cut(s) 3328, 5263
EcoT38I GRGCYC 3 cut(s) 132, 4668, 5404
EgeI GGCGCC 1 cut(s) 3622
EheI GGCGCC 1 cut(s) 3622
ErhI CCWWGG 3 cut(s) 2619, 4213, 5327
Esp3I CGTCTC 1 cut(s) 3956
FalI AAGNNNNNCTT 6 cut(s) 389, 421, 1692, 1724, 2057, 2089
FauI CCCGC 5 cut(s) 1822, 2457, 3172, 3691, 4075
FauNDI CATATG 2 cut(s) 777, 3307
FbaI TGATCA 6 cut(s) 115, 190, 1395, 1539, 2011, 4924
FblI GTMKAC 3 cut(s) 341, 2956, 5449
FriOI GRGCYC 3 cut(s) 132, 4668, 5404
GlaI GCGC 3 cut(s) 3622, 4211, 4296
GsaI CCCAGC 5 cut(s) 26, 1869, 2416, 4716, 4922
HaeII RGCGCY 1 cut(s) 3624
HaeIII GGCC 9 cut(s) 935, 1060, 1662, 2618, 3880, 4280, 4362, 5324, 5436
HapII CCGG 4 cut(s) 2176, 2660, 3359, 5321
HgaI GACGC 1 cut(s) 562
HhaI GCGC 3 cut(s) 3623, 4212, 4297
Hin1I GRCGYC 2 cut(s) 573, 3621
Hin6I GCGC 3 cut(s) 3621, 4210, 4295
HinP1I GCGC 3 cut(s) 3621, 4210, 4295
HincII GTYRAC 2 cut(s) 3682, 5450
HindII GTYRAC 2 cut(s) 3682, 5450
HindIII AAGCTT 4 cut(s) 1336, 1740, 3963, 4514
HpaII CCGG 4 cut(s) 2176, 2660, 3359, 5321
HphI GGTGA 5 cut(s) 1181, 1496, 1882, 2684, 2951
Hpy166II GTNNAC 9 cut(s) 342, 899, 1257, 1567, 2957, 3014, 3356, 3682, 5450
Hpy8I GTNNAC 9 cut(s) 342, 899, 1257, 1567, 2957, 3014, 3356, 3682, 5450
Hpy99I CGWCG 2 cut(s) 1140, 2948
HpyCH4IV ACGT 8 cut(s) 1138, 1201, 1725, 1894, 2680, 4396, 4411, 5317
HpySE526I ACGT 8 cut(s) 1138, 1201, 1725, 1894, 2680, 4396, 4411, 5317
Hsp92I GRCGYC 2 cut(s) 573, 3621
HspAI GCGC 3 cut(s) 3621, 4210, 4295
KasI GGCGCC 1 cut(s) 3620
Kpn2I TCCGGA 1 cut(s) 2659
Ksp22I TGATCA 6 cut(s) 115, 190, 1395, 1539, 2011, 4924
LguI GCTCTTC 2 cut(s) 1512, 3306
LmnI GCTCC 3 cut(s) 135, 836, 4813
MaeII ACGT 8 cut(s) 1138, 1201, 1725, 1894, 2680, 4396, 4411, 5317
MbiI CCGCTC 1 cut(s) 2633
MfeI CAATTG 7 cut(s) 892, 1386, 3771, 4007, 4560, 4854, 4905
MflI RGATCY 5 cut(s) 595, 1218, 2962, 3340, 4267
MhlI GDGCHC 9 cut(s) 29, 132, 155, 901, 1259, 3301, 3358, 4668, 5404
MlsI TGGCCA 1 cut(s) 5436
MluNI TGGCCA 1 cut(s) 5436
Mly113I GGCGCC 1 cut(s) 3621
MlyI GAGTC 3 cut(s) 914, 3418, 4506
MmeI TCCRAC 6 cut(s) 310, 604, 2326, 3368, 3873, 5156
Mox20I TGGCCA 1 cut(s) 5436
Mph1103I ATGCAT 2 cut(s) 3328, 5263
MroI TCCGGA 1 cut(s) 2659
MroXI GAANNNNTTC 4 cut(s) 333, 984, 2539, 4170
MscI TGGCCA 1 cut(s) 5436
MslI CAYNNNNRTG 3 cut(s) 123, 1572, 2778
Msp20I TGGCCA 1 cut(s) 5436
MspA1I CMGCKG 7 cut(s) 958, 1459, 1714, 2342, 2693, 3626, 4922
MspCI CTTAAG 1 cut(s) 4187
MspI CCGG 4 cut(s) 2176, 2660, 3359, 5321
MunI CAATTG 7 cut(s) 892, 1386, 3771, 4007, 4560, 4854, 4905
Mva1269I GAATGC 6 cut(s) 735, 1867, 3550, 3729, 5175, 5263
MvnI CGCG 2 cut(s) 1859, 2108
NarI GGCGCC 1 cut(s) 3621
NdeI CATATG 2 cut(s) 777, 3307
NlaIV GGNNCC 6 cut(s) 783, 1921, 3342, 3622, 5117, 5325
NmeAIII GCCGAG 1 cut(s) 3771
NmuCI GTSAC 8 cut(s) 566, 574, 1688, 2211, 2672, 2939, 3385, 5192
NruI TCGCGA 1 cut(s) 1859
NsiI ATGCAT 2 cut(s) 3328, 5263
NspI RCATGY 2 cut(s) 3326, 3979
NspV TTCGAA 1 cut(s) 2842
OliI CACNNNNGTG 1 cut(s) 1572
PaeI GCATGC 1 cut(s) 3326
PaqCI CACCTGC 1 cut(s) 2583
PceI AGGCCT 2 cut(s) 1662, 3880
PciI ACATGT 1 cut(s) 3975
PciSI GCTCTTC 2 cut(s) 1512, 3306
PcsI WCGNNNNNNNCGW 1 cut(s) 4528
PctI GAATGC 6 cut(s) 735, 1867, 3550, 3729, 5175, 5263
PdmI GAANNNNTTC 4 cut(s) 333, 984, 2539, 4170
PflFI GACNNNGTC 1 cut(s) 572
PflMI CCANNNNNTGG 1 cut(s) 292
PfoI TCCNGGA 1 cut(s) 3279
PinAI ACCGGT 1 cut(s) 3358
PleI GAGTC 3 cut(s) 913, 3417, 4505
PluTI GGCGCC 1 cut(s) 3624
PpsI GAGTC 3 cut(s) 913, 3417, 4505
Ppu21I YACGTR 1 cut(s) 1895
PpuMI RGGWCCY 2 cut(s) 353, 4798
PscI ACATGT 1 cut(s) 3975
Psp124BI GAGCTC 1 cut(s) 132
Psp5II RGGWCCY 2 cut(s) 353, 4798
PspFI CCCAGC 5 cut(s) 22, 1865, 2412, 4712, 4918
PspN4I GGNNCC 6 cut(s) 783, 1921, 3342, 3622, 5117, 5325
PspPI GGNCC 6 cut(s) 353, 934, 2616, 4590, 4798, 5323
PspPPI RGGWCCY 2 cut(s) 353, 4798
PstNI CAGNNNCTG 1 cut(s) 260
PsuI RGATCY 5 cut(s) 595, 1218, 2962, 3340, 4267
PsyI GACNNNGTC 1 cut(s) 572
PvuII CAGCTG 5 cut(s) 958, 1459, 2342, 2693, 4922
RruI TCGCGA 1 cut(s) 1859
RseI CAYNNNNRTG 3 cut(s) 123, 1572, 2778
SacI GAGCTC 1 cut(s) 132
SalI GTCGAC 1 cut(s) 5448
SapI GCTCTTC 2 cut(s) 1512, 3306
Sau96I GGNCC 6 cut(s) 353, 934, 2616, 4590, 4798, 5323
ScaI AGTACT 1 cut(s) 1144
SchI GAGTC 3 cut(s) 914, 3418, 4506
SduI GDGCHC 9 cut(s) 29, 132, 155, 901, 1259, 3301, 3358, 4668, 5404
SfcI CTRYAG 2 cut(s) 786, 1570
SfoI GGCGCC 1 cut(s) 3622
SfuI TTCGAA 1 cut(s) 2842
SinI GGWCC 3 cut(s) 353, 4590, 4798
SmiMI CAYNNNNRTG 3 cut(s) 123, 1572, 2778
SmlI CTYRAG 5 cut(s) 1664, 2291, 3953, 4187, 4482
SmoI CTYRAG 5 cut(s) 1664, 2291, 3953, 4187, 4482
SpeI ACTAGT 1 cut(s) 2906
SphI GCATGC 1 cut(s) 3326
SseBI AGGCCT 2 cut(s) 1662, 3880
SspDI GGCGCC 1 cut(s) 3620
SspI AATATT 3 cut(s) 1240, 3456, 4138
SstI GAGCTC 1 cut(s) 132
StuI AGGCCT 2 cut(s) 1662, 3880
StyI CCWWGG 3 cut(s) 2619, 4213, 5327
TaiI ACGT 8 cut(s) 1141, 1204, 1728, 1897, 2683, 4399, 4414, 5320
TaqI TCGA 7 cut(s) 1481, 1908, 2842, 3424, 4286, 4504, 5449
TatI WGTACW 5 cut(s) 318, 383, 1142, 4617, 5014
TauI GCSGC 4 cut(s) 266, 660, 2009, 3626
TseFI GTSAC 8 cut(s) 566, 574, 1688, 2211, 2672, 2939, 3385, 5192
Tsp45I GTSAC 8 cut(s) 566, 574, 1688, 2211, 2672, 2939, 3385, 5192
TspGWI ACGGA 4 cut(s) 3722, 3938, 4031, 4640
Tth111I GACNNNGTC 1 cut(s) 572
Van91I CCANNNNNTGG 1 cut(s) 292
Vha464I CTTAAG 1 cut(s) 4187
VneI GTGCAC 3 cut(s) 897, 1255, 3354
VpaK11BI GGWCC 3 cut(s) 353, 4590, 4798
XagI CCTNNNNNAGG 1 cut(s) 5331
XceI RCATGY 2 cut(s) 3326, 3979
XcmI CCANNNNNNNNNTGG 2 cut(s) 2780, 4033
XmaJI CCTAGG 1 cut(s) 5327
XmiI GTMKAC 3 cut(s) 341, 2956, 5449
XmnI GAANNNNTTC 4 cut(s) 333, 984, 2539, 4170
ZrmI AGTACT 1 cut(s) 1144
Zsp2I ATGCAT 2 cut(s) 3328, 5263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.