RLG00000021588

Sister chromatid cohesion protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010273
Physical Location & Seq
Forward (+)
76612569 .. 76624187
11619 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021588

Sequence Viewer

Length: 5409 bp
ATGAGCTATCCGGTCAGTTCGAGCTCTGGCTCCGGCGCCAGCCACCAAGGCATCCGGCTCTCCAATACTACTCACTCCGAGGTCGCTCCTTGCTTGCCTTTGCCTTCGCTTCCGGTGTTCTGCGGCGCTTCCGATCAGGACCTCCGGTTGTTCGATGAGCCGAGTCGGAGCAGTGCGAGGTTGAATTACTACGACGCAGCTCAGGCCAGTAGGATTGCTGATCTGCTTCGAGCTACTGACGTCTCTTACTTGAACCTGAGGGAGGATACTGCGGCGGTTCAATATGGCTATGTCCAACCTATGGAACTTCACGATGAAGTTTTACAGTACAATCCAGAAGCCTTTCAGTATACTACTCCAGGTCCTATTATGGGGCAAGTGCCTGGCAGTAGTACAGTACCAGATAAGAAGCCCTTTGTGCCAGTTATACCTATAACAAGAACTTCTGAAAGAGATTATGGTGCAACTCACAATAATCAGCTCAACTATACTCCTCCCAATGACATATCTATACCATCCTCCAGAAAACCAAAAGCAAAGAGAAAAGCTAGTGATGGCGTTACTTCCTCAGTTGGGTCAGATCCCGTTGCAAGTCAAGAAGCCACTATTGGATGCTTCTGTGAGATGGTGGAGGATTTTTGCGGCAGAGCTGAAATTTTCAGTGAGGATCGGGACGAAGCACAGTGGTTATCTATGCCTCTTTCTGATCTTAGAATGCTTGCAAATGAAATAATGTCTCTTCGTGCAAAGAGGCTTCTACATATGGTTCCTGTAGATACTTTTGTTAGATTGTTGAGGATTTTAGATCATCAGATACATGGAGCTGAAGGCTTGTCTATAAATGAACAGTCAGATTCAAATGGGGTTTCATCAATCAATTGTGCACTAGAGTCCATTCATGCAGCTTTAGCAGTAATGGCCCATAACCAGATGCCAAAGCAGCTGTATAAAGAAGAGATCATTGAAAGAATTCTGGAGTTCTCCAGGCATCAGATAATGGATGTTATGTGTGCTTGCGATCCATCATATCGTGCTTTACATAGGCCAAGTGAAAATGGGACACTTGAAGTTGAGGAAGACGAAATTCTTGATGCTGAAACTGGTTCAGCAAGCAAGAGGCGACGTGGTACTAAGACTGTTAAAGTGCAGAAATCATCATATAACAGGGTCTCTGCTGCTGTGAATAATATTCTTCAGAAAATGTGCACAATTCTTGGTTTGCTAAAGGATTTGTTGCTGATAGAAAAGTTGTCAGATAGTTGTATCTTACAACTACTGAAAACAAGCTTTACAACATTTATGGTGGATAATATCCAGCTTCTGCAACTGAAGGCAATTGGTTTGATCAGTGGGATATACTATTCATACACTCAACATCGGACTTATGTTATAGATGAGTTACTTCAGCTGCTTTGGAAGTTGCCTTTCTCGAAGCGAGCTTTGAGAGCCTATCACCTACCTGATGAAGAGCAAACGCAGATTCAAATGATCACTGCTTTGCTGATTCAGTTGGTTCACTGTAGTGCAAACCTTCCTGCACCTTTAAGGCAGGATTCAAGTGTTAATTCCATCTTAGAAGTTTCTGACAATGCTGATTATCCAATTAAAGGCCTTGAGGCAGCACAAGCAGCATGTTGTCACTTTTGGAAAGGGGTACTTCAGCGGTTTGCTAATGTCAAGAACCAGGAAGCTTCAGAATTCAAAGTTATGATGGAGAATCTTGTTACTGATCTGCTGACAACATTGAATTTACCTGAATATCCCGCTTCAGCCCCTATTCTGGAGGTTCTTTGTGTCTTGTTACTCGCGAATGCTGGGGTGAAATCTAAAGATGTAGCTGCACGTAGCATGGCTATCGACCTTCTTGGAACCATTGCTGCGAGGTTGAAGCGTGACTCTCTCCTTCGCATTAGGGGCAAATTTTGGATATTACAGGAGTTGGTTAGTGGGGATGCGGCTGATCAGACTTATCCAAAAGATGTATGCTCTGCTTGTTTAGATGGCAGGGTTGAAAAGACTTTCTTTGTATGTCAAGGTTGTCAGAGAATGTTTCACGCAGATTGCATGGGAGTAAGAGAGCATGAAGTTCCTAACCAATCTTGGCACTGTCAGATATGCCTCTGCCAGAAACAACTTCTTGTTTTACAATCTTACTGCAAGTCACAATACAAAGATGATGGATTTAAGGATCATAAAAGTTCAGGAAGAAAAACTGAAGTTACTTTTTCAATTACTAAACTTGAGATCGTCCAACAGTTGCTTTTGAATTACCTACAAGATACCGCATCAGCTGATGATGTTCATCTCTTTGTTCGTTGGCTTTATGTCTGCTTGTGGTACAAGGATGATCCACCAAAGTCCCAGCAGAAGTTCATTTACTATCTTGCAAGACTGAACTCAAAAGCAATAGTGCGGGATTCTGGGACTGTTTTTTCCTTGCTGACAAGGGACTTAATCAAGCAGATTACCTTAGTATTGGGACGAAATACTTCTTTTGCCAGAGGATTTGATAAGATTCTACACCTGCTTCTGGCAAGTCTAAGGGAGAATTCTCCTGTAATCAGGGCCAAGGCTTTACGAGCGGTTAGTATAGTTGTAGAGGCTGATCCGGAGGTTTTAGGTGACAAACGTGTGCAACCAGCTGTTGAAGGAAGGTTTTGTGATTCTGCAATTTCTGTTAGAGAAGCAGCACTGGAACTCGTAGGGAGGCATATTGCTTCCCATCCTGATGTTGGTTTGAAGTATTTTGAGAAGGTTGCTGAGAGAATCAAAGATACGGGTGTGAGTGTTCGAAAGCGATCTATTAAAATCATACGAGATATGTGCGTTTCAAATGGGGACTTCTCTGAATTTACTAGTGCTTGCATTGCTATCATCTCCCGTATTGGTGACGATGAATCAAGTATACAGGATCTTGTTTGCAAGACATTTTATGAGTTCTGGTTTGAGGAACATACTGGTTCACATACTCAATTCTTTGGAGATGATAGCTCTGTTCCATTGGAGGTGGCTAAAAAAACTGAACAGATTGTTGAGATGTTGAGGAGGATGCCAACACCACACCATCTTGTTACCGTCATTAAACGCAACTTAGCCCTTGATTTTTTCCCACAAGCAGCTAAAGCTGCGGGAATCAATCCCGTCTTACTTGCATCAGTACGCAATCGGTGTGAGCTAATGTGCAAGTTTTTACTGGAAAGGATATTGCAGGTAGAAGAAATGAATATCCAGGAAGTTGAGATGCGTGCACTTCCATATGTGCAAGTTCTGCATGCATTTTGTGTTGTGGATCCAACATTATGTGCACCGGTTTCTAACCCTTCGCAGTTTGTGGTCACTCTTCAGCCTTATCTAAAGAGTCAGGATGATAATCGAGTGGTTGCCAAGTTATTGGAGAGCATAATATTCATAATTGATTCTGTCCTCCCTTTAGTAAGAAAGTTGCCACAAAATGTTCTTGAAGAACTTGAACAAGACTTAAAGAGCATGATTGTTCGGCATTCCTTCTTGACAGTTGTTCATGCTTGCATCAAGTGCCTCTGTGCTGTGAGTAAAGTGGCAGGAAAAGGCGCTGCTGTTGTGGAGTATCTAATTCAGGTTTTTTACAAACGGCTGGATGCTGAGGAAGTTGACAACCAGCAGAAAGCGGGGCGATCTCTTTTCTGTCTTGGAATGCTTATCCGTTATGGCAACTCTTTACTGTGTTACTCTGACCAAACAATTGATGTTGCAAGCAGTCTCAGCTTATTTAAAAGATATCTTCTAATGGATGATTTTTTCTTAAAGGCTAGATCATTGCAGGCATTGGGCTTTGTGTTAATTGCTAGGCCTGAATTTATGTTGGAAAAAGATATTGGGAAGATATTAGAGGAGACATTCTCTTCTAGCTCTGATGTCCGTCTCAAGATGCAAGCTTTGCAAAACATGTACGACTATCTTCTTGATGCTGAAACTCAATTGGGAACGGATACAACCAGTAATAATGTGGCTGATTATTCTGTGGAGGGTGGCAATGCTGTACCTGTTGCTGCGGGTGCTGGAGATACTAATATATGTGGCGGAATAGTTCAGTTGTATTGGGATAATATTCTGGAGAGATGTTTGGACTTTAATGAACAAATTCGCAGCTCTGCCCTTAAGATTGTTGAAGTTGTGCTGCGCCAAGGGCTTGTGCATCCTATTACTTGTGTTCCCTTCCTTATTGCACTGGAAACAGATCCACTGGAGGCCAATTCGACATTAGCACATCATTTGCTGATGAATATGAATGAAAAGTACCCTTCATTTTTTGAAAGCCGACTAGGGGATGGCCTTCAAATGTCTTTTGGATTTATACAATCCATACGTCCTGGTACTGAATGTGAGAACACAAAACCACCATCAAAGGCTTCTGGAAATGCAAAGGGAAAAGTTGATGATGTTTCTTTTGCTCAAGCAAGATTGGGAGTCTCTCGAATCTACAAGCTTATTCGTGCTAACCGTGTTTCAAGGAACAAATTTATGTCCTCAATTGTTCGCAAGTTTGATAATCCTAGCTGGACCACTTCAGTAGTCCCTTTCCTCATGTACTGCACGGAAATACTTGCCTTGCTGCCATTCACAACACCTGATGAGCCCCTTTATTTAGTCTATGCTATAAATAGAGTAATACAAGTTAAGGCTGGGCAACTTGAAGCAAAGCTGAAAGCTCTGACATTGCATCTGTTACAAAGAGGTGCACCCCGTGGGAATGGCATAATTAAAGAGGACCCAGCTGCTCCGCCCTTTACAAGAGGAATGGCATCAGTGGATTTGAATGGGACAATTGAATCAGAACCAGCTTATCACATGGCAGCTATGGATTTGAATGGGATGATTGAACAAGACCCAGCTGATCAGTCTGTCTCAAATCAAGATACCGTGTTAGAGGGAAAGATGCCCGGTAAAGGTTCTGTTAGTTCCTCTGGCATCTCAATAGATGATGTACAGATAATCCAGGCAGACTGCCTGGCAGCTATTGCATTGCAGCTTCTTTTGAAACTGAAGAGACACCTAAAAATTGTTTATAGCTTGAATGATGCTAGGTGCCAGGCTTTCTCTCCAACCGATCCCATTAAACCTGGAGATGCTTTCTCCAAGCAGAGCATTCCTTTTGACGTCAGTGACACGCATACTAACTTGCCTGGAACTTATCAAGAATTGGTACAAAGATATCAGGAATTCAAGAATGCGTTGAAGGATGATACAGTTGATTTCTCAACTTACACAGCAAATCTCAAAAGAAAACGTCCGGCCCCTAGGAAAGGAAGGAAATCTGGAGTTAGAGATGATGAGGATGACAATGATGATGATGAAGATTGGTCAGGTGGGGCTCGGAGGCTGAGTTATAGTGGGAGGAGAGGTGGCCACAGTAGAAGTCGACAACGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000070 GO:0000122 GO:0000278 GO:0000280 GO:0000428 GO:0000819 GO:0001654 GO:0001655 GO:0001656 GO:0001822 GO:0003006 GO:0003007 GO:0003008 GO:0003151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007064 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0007423 GO:0007507 GO:0007548 GO:0007600 GO:0007605 GO:0008022 GO:0008104 GO:0008150 GO:0009314 GO:0009628 GO:0009653 GO:0009790 GO:0009887 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010165 GO:0010171 GO:0010212 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010720 GO:0016043 GO:0016591 GO:0019219 GO:0019222 GO:0019899 GO:0019904 GO:0022008 GO:0022402 GO:0022414 GO:0030154 GO:0030326 GO:0030334 GO:0030335 GO:0030880 GO:0031056 GO:0031058 GO:0031063 GO:0031065 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032039 GO:0032116 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0033365 GO:0033554 GO:0034085 GO:0034086 GO:0034088 GO:0034502 GO:0034613 GO:0035107 GO:0035108 GO:0035112 GO:0035113 GO:0035115 GO:0035136 GO:0035239 GO:0035261 GO:0035295 GO:0040007 GO:0040008 GO:0040012 GO:0040017 GO:0042471 GO:0042634 GO:0042826 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043388 GO:0043583 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045597 GO:0045892 GO:0045934 GO:0045995 GO:0047485 GO:0048285 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048546 GO:0048557 GO:0048562 GO:0048565 GO:0048566 GO:0048568 GO:0048589 GO:0048592 GO:0048598 GO:0048608 GO:0048638 GO:0048699 GO:0048731 GO:0048736 GO:0048806 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0050896 GO:0050954 GO:0051094 GO:0051098 GO:0051099 GO:0051101 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051253 GO:0051270 GO:0051272 GO:0051276 GO:0051641 GO:0051716 GO:0051960 GO:0051962 GO:0055029 GO:0055123 GO:0060065 GO:0060173 GO:0060255 GO:0060284 GO:0060322 GO:0060323 GO:0060324 GO:0060325 GO:0061008 GO:0061010 GO:0061038 GO:0061458 GO:0061695 GO:0065007 GO:0065009 GO:0070013 GO:0070087 GO:0070727 GO:0071168 GO:0071214 GO:0071478 GO:0071479 GO:0071481 GO:0071840 GO:0071921 GO:0072001 GO:0072359 GO:0080090 GO:0090311 GO:0090312 GO:0090596 GO:0090694 GO:0098813 GO:0104004 GO:0140014 GO:1902275 GO:1902494 GO:1902679 GO:1903047 GO:1903506 GO:1903507 GO:1905269 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2000145 GO:2000147 GO:2001141 GO:2001222 GO:2001224 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04111 map04111
Pfam Domains
Protein Families
Cohesin_HEAT Nipped-B_C PHD

Protein Analysis

1803

Amino Acids

201.19

Weight (kDa)

6.26

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cohesin_HEAT PF12765 860 - 901 1.2e-12 HEAT repeat associated with sister chromatid cohesion
Nipped-B_C PF12830 1352 - 1549 2.4e-51 Sister chromatid cohesion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15540 AT5G15540
fragaria_vesca FvH4_6g47080 FvH4_6g47080 FvH4_6g47080
malus_domestica MD09G1064300.v1.1 MD17G1057600.v1.1
prunus_persica Prupe.3G256700_v2.0.a1
pyrus_communis pycom111g05400 pycom17g05770
rosa_chinensis RchiOBHm_Chr2g0166131
rosa_laevigata RLG00000021588
rosa_multiflora Rmu_sc0006218.1_g000028
rosa_roxburghii Rroxscaffold_2G00085180
rosa_rugosa Rorug02G0520500 Rorug02G0520600
rosa_samantha Rh2AG586600 Rh2BG598400 Rh2CG569100 Rh2DG608300
rosa_wichuraiana Rw2G048900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2532
AatII GACGTC 2 cut(s) 243, 5139
Acc36I ACCTGC 2 cut(s) 2532, 3199
AccB1I GGYRCC 2 cut(s) 35, 5064
AccB7I CCANNNNNTGG 1 cut(s) 301
AccBSI CCGCTC 1 cut(s) 2582
AccI GTMKAC 3 cut(s) 350, 2905, 5398
AccII CGCG 1 cut(s) 1808
AccIII TCCGGA 1 cut(s) 2608
AcoI YGGCCR 1 cut(s) 5383
AcyI GRCGYC 3 cut(s) 36, 240, 5136
AdeI CACNNNGTG 1 cut(s) 4724
AflII CTTAAG 1 cut(s) 4136
AflIII ACRYGT 2 cut(s) 2629, 3924
AgeI ACCGGT 1 cut(s) 3307
AhlI ACTAGT 1 cut(s) 2855
AjiI CACGTC 1 cut(s) 1124
AjuI GAANNNNNNNTTGG 4 cut(s) 591, 623, 3837, 3869
AleI CACNNNNGTG 1 cut(s) 1521
Alw21I GWGCWC 6 cut(s) 26, 886, 1208, 3250, 3307, 4720
Alw26I GTCTC 9 cut(s) 247, 741, 1174, 3743, 3866, 3905, 4453, 4888, 5020
Alw44I GTGCAC 5 cut(s) 882, 1204, 3246, 3303, 4716
AlwNI CAGNNNCTG 1 cut(s) 1321
Aor13HI TCCGGA 1 cut(s) 2608
ApaLI GTGCAC 5 cut(s) 882, 1204, 3246, 3303, 4716
AsiGI ACCGGT 1 cut(s) 3307
Asp700I GAANNNNTTC 4 cut(s) 342, 969, 2488, 4119
AspA2I CCTAGG 1 cut(s) 5276
AspLEI GCGC 4 cut(s) 38, 128, 3572, 4161
AspS9I GGNCC 7 cut(s) 139, 362, 919, 2565, 4539, 4747, 5272
AsuC2I CCSGG 1 cut(s) 4920
AsuHPI GGTGA 4 cut(s) 1445, 1831, 2633, 2900
AsuII TTCGAA 1 cut(s) 2791
AvaII GGWCC 4 cut(s) 139, 362, 4539, 4747
AvrII CCTAGG 1 cut(s) 5276
AxyI CCTNAGG 1 cut(s) 257
BaeGI GKGCMC 5 cut(s) 886, 1208, 3250, 3307, 4720
BaeI ACNNNNGTAYC 4 cut(s) 768, 801, 2766, 2799
BalI TGGCCA 1 cut(s) 5385
BamHI GGATCC 1 cut(s) 3289
BanI GGYRCC 2 cut(s) 35, 5064
BanII GRGCYC 3 cut(s) 26, 4617, 5353
BarI GAAGNNNNNNTAC 2 cut(s) 2360, 2392
BbsI GAAGAC 1 cut(s) 1083
Bbv12I GWGCWC 6 cut(s) 26, 886, 1208, 3250, 3307, 4720
BbvCI CCTCAGC 1 cut(s) 3621
BceAI ACGGC 1 cut(s) 3626
BcgI CGANNNNNNTGC 4 cut(s) 1837, 1871, 2805, 2839
BciVI GTATCC 2 cut(s) 259, 3961
BclI TGATCA 4 cut(s) 1344, 1488, 1960, 4873
BcnI CCSGG 1 cut(s) 4920
BcoDI GTCTC 9 cut(s) 247, 741, 1174, 3743, 3866, 3905, 4453, 4888, 5020
BcuI ACTAGT 1 cut(s) 2855
BfmI CTRYAG 2 cut(s) 771, 1519
BfoI RGCGCY 3 cut(s) 39, 129, 3573
BfrI CTTAAG 1 cut(s) 4136
BfuAI ACCTGC 2 cut(s) 2532, 3199
BfuI GTATCC 2 cut(s) 259, 3961
BglI GCCNNNNNGGC 1 cut(s) 48
BlnI CCTAGG 1 cut(s) 5276
Bme18I GGWCC 4 cut(s) 139, 362, 4539, 4747
BmgBI CACGTC 1 cut(s) 1124
BmgT120I GGNCC 7 cut(s) 139, 362, 919, 2565, 4539, 4747, 5272
BmiI GGNNCC 8 cut(s) 31, 37, 768, 1870, 3291, 4749, 5066, 5274
BpiI GAAGAC 1 cut(s) 1083
BplI GAGNNNNNCTC 2 cut(s) 3863, 3895
Bpu10I CCTNAGC 2 cut(s) 201, 3621
Bpu14I TTCGAA 1 cut(s) 2791
BpuEI CTTGAG 4 cut(s) 1634, 2261, 3887, 4416
BpuMI CCSGG 1 cut(s) 4920
BsaAI YACGTR 1 cut(s) 1844
BsaBI GATNNNNATC 2 cut(s) 2301, 3369
BsaHI GRCGYC 3 cut(s) 36, 240, 5136
BsaI GGTCTC 1 cut(s) 1174
BsaJI CCNNGG 6 cut(s) 46, 78, 2568, 4162, 4723, 5276
BsaWI WCCGGW 5 cut(s) 10, 112, 144, 2608, 3307
BsaXI ACNNNNNCTCC 4 cut(s) 5347, 5365, 5377, 5395
Bse118I RCCGGY 1 cut(s) 3307
Bse1I ACTGG 9 cut(s) 207, 422, 1105, 2697, 2962, 3198, 3975, 4212, 4227
Bse21I CCTNAGG 1 cut(s) 257
Bse3DI GCAATG 6 cut(s) 1872, 2865, 3794, 4018, 4694, 5000
Bse8I GATNNNNATC 2 cut(s) 2301, 3369
BseAI TCCGGA 1 cut(s) 2608
BseDI CCNNGG 6 cut(s) 46, 78, 2568, 4162, 4723, 5276
BseJI GATNNNNATC 2 cut(s) 2301, 3369
BseMI GCAATG 6 cut(s) 1872, 2865, 3794, 4018, 4694, 5000
BseMII CTCAG 7 cut(s) 215, 248, 582, 2751, 3612, 3754, 5351
BseNI ACTGG 9 cut(s) 207, 422, 1105, 2697, 2962, 3198, 3975, 4212, 4227
BseRI GAGGAG 4 cut(s) 483, 3058, 3884, 5389
BseSI GKGCMC 5 cut(s) 886, 1208, 3250, 3307, 4720
BseYI CCCAGC 5 cut(s) 1814, 2361, 4661, 4750, 4867
BsgI GTGCAG 4 cut(s) 1166, 1521, 1824, 4555
Bsh1236I CGCG 1 cut(s) 1808
BshNI GGYRCC 2 cut(s) 35, 5064
BshTI ACCGGT 1 cut(s) 3307
BsiHKAI GWGCWC 6 cut(s) 26, 886, 1208, 3250, 3307, 4720
BsiSI CCGG 9 cut(s) 11, 33, 55, 113, 145, 2609, 3308, 4920, 5270
BslFI GGGAC 9 cut(s) 686, 1072, 2344, 2437, 2462, 2493, 2852, 4538, 4813
BsmAI GTCTC 9 cut(s) 247, 741, 1174, 3743, 3866, 3905, 4453, 4888, 5020
BsmBI CGTCTC 2 cut(s) 247, 3905
BsmFI GGGAC 9 cut(s) 686, 1072, 2344, 2437, 2462, 2493, 2852, 4538, 4813
BsmI GAATGC 6 cut(s) 720, 1816, 3499, 3678, 5124, 5212
Bso31I GGTCTC 1 cut(s) 1174
Bsp119I TTCGAA 1 cut(s) 2791
Bsp1286I GDGCHC 8 cut(s) 26, 886, 1208, 3250, 3307, 4617, 4720, 5353
Bsp13I TCCGGA 1 cut(s) 2608
Bsp1407I TGTACA 1 cut(s) 4963
Bsp68I TCGCGA 1 cut(s) 1808
BspCNI CTCAG 7 cut(s) 214, 249, 581, 2752, 3613, 3753, 5352
BspEI TCCGGA 1 cut(s) 2608
BspFNI CGCG 1 cut(s) 1808
BspLI GGNNCC 8 cut(s) 31, 37, 768, 1870, 3291, 4749, 5066, 5274
BspMI ACCTGC 2 cut(s) 2532, 3199
BspQI GCTCTTC 1 cut(s) 1461
BspT104I TTCGAA 1 cut(s) 2791
BspT107I GGYRCC 2 cut(s) 35, 5064
BspTI CTTAAG 1 cut(s) 4136
BspTNI GGTCTC 1 cut(s) 1174
BsrBI CCGCTC 1 cut(s) 2582
BsrDI GCAATG 6 cut(s) 1872, 2865, 3794, 4018, 4694, 5000
BsrFI RCCGGY 1 cut(s) 3307
BsrGI TGTACA 1 cut(s) 4963
BsrI ACTGG 9 cut(s) 207, 422, 1105, 2697, 2962, 3198, 3975, 4212, 4227
BssAI RCCGGY 1 cut(s) 3307
BssECI CCNNGG 6 cut(s) 46, 78, 2568, 4162, 4723, 5276
BssNAI GTATAC 2 cut(s) 351, 2906
BssNI GRCGYC 3 cut(s) 36, 240, 5136
BssT1I CCWWGG 4 cut(s) 46, 2568, 4162, 5276
Bst1107I GTATAC 2 cut(s) 351, 2906
Bst6I CTCTTC 6 cut(s) 744, 948, 1461, 3345, 3886, 5018
BstACI GRCGYC 3 cut(s) 36, 240, 5136
BstAFI CTTAAG 1 cut(s) 4136
BstAPI GCANNNNNTGC 4 cut(s) 3268, 3534, 3916, 4997
BstAUI TGTACA 1 cut(s) 4963
BstBAI YACGTR 1 cut(s) 1844
BstBI TTCGAA 1 cut(s) 2791
BstDSI CCRYGG 1 cut(s) 4723
BstENI CCTNNNNNAGG 2 cut(s) 260, 5280
BstFNI CGCG 1 cut(s) 1808
BstH2I RGCGCY 3 cut(s) 39, 129, 3573
BstHHI GCGC 4 cut(s) 38, 128, 3572, 4161
BstMAI GTCTC 9 cut(s) 247, 741, 1174, 3743, 3866, 3905, 4453, 4888, 5020
BstNSI RCATGY 3 cut(s) 1635, 3275, 3928
BstSFI CTRYAG 2 cut(s) 771, 1519
BstSLI GKGCMC 5 cut(s) 886, 1208, 3250, 3307, 4720
BstUI CGCG 1 cut(s) 1808
BstV2I GAAGAC 1 cut(s) 1083
BstX2I RGATCY 4 cut(s) 580, 2911, 3289, 4216
BstXI CCANNNNNNTGG 1 cut(s) 3391
BstYI RGATCY 4 cut(s) 580, 2911, 3289, 4216
BstZ17I GTATAC 2 cut(s) 351, 2906
Bsu36I CCTNAGG 1 cut(s) 257
BsuI GTATCC 2 cut(s) 259, 3961
BtgI CCRYGG 1 cut(s) 4723
BtrI CACGTC 1 cut(s) 1124
BtsI GCAGTG 2 cut(s) 178, 1491
BtuMI TCGCGA 1 cut(s) 1808
BveI ACCTGC 2 cut(s) 2532, 3199
CaiI CAGNNNCTG 1 cut(s) 1321
CfoI GCGC 4 cut(s) 38, 128, 3572, 4161
Cfr10I RCCGGY 1 cut(s) 3307
Cfr13I GGNCC 7 cut(s) 139, 362, 919, 2565, 4539, 4747, 5272
CseI GACGC 1 cut(s) 203
CspAI ACCGGT 1 cut(s) 3307
DinI GGCGCC 1 cut(s) 37
DraI TTTAAA 1 cut(s) 3751
DraIII CACNNNGTG 1 cut(s) 4724
EaeI YGGCCR 1 cut(s) 5383
Eam1104I CTCTTC 6 cut(s) 744, 948, 1461, 3345, 3886, 5018
EarI CTCTTC 6 cut(s) 744, 948, 1461, 3345, 3886, 5018
EciI GGCGGA 2 cut(s) 4074, 4749
Ecl136II GAGCTC 1 cut(s) 24
Eco130I CCWWGG 4 cut(s) 46, 2568, 4162, 5276
Eco147I AGGCCT 2 cut(s) 1611, 3829
Eco24I GRGCYC 3 cut(s) 26, 4617, 5353
Eco31I GGTCTC 1 cut(s) 1174
Eco32I GATATC 2 cut(s) 3758, 5192
Eco47I GGWCC 4 cut(s) 139, 362, 4539, 4747
Eco53kI GAGCTC 1 cut(s) 24
Eco81I CCTNAGG 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 24
EcoNI CCTNNNNNAGG 2 cut(s) 260, 5280
EcoO109I RGGNCCY 3 cut(s) 139, 362, 4747
EcoRI GAATTC 4 cut(s) 969, 1697, 2548, 5198
EcoRV GATATC 2 cut(s) 3758, 5192
EcoT14I CCWWGG 4 cut(s) 46, 2568, 4162, 5276
EcoT22I ATGCAT 1 cut(s) 3277
EcoT38I GRGCYC 3 cut(s) 26, 4617, 5353
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 4 cut(s) 46, 2568, 4162, 5276
Esp3I CGTCTC 2 cut(s) 247, 3905
FalI AAGNNNNNCTT 6 cut(s) 398, 430, 1641, 1673, 2006, 2038
FaqI GGGAC 9 cut(s) 686, 1072, 2344, 2437, 2462, 2493, 2852, 4538, 4813
FauI CCCGC 5 cut(s) 1771, 2406, 3121, 3640, 4024
FauNDI CATATG 2 cut(s) 762, 3256
FbaI TGATCA 4 cut(s) 1344, 1488, 1960, 4873
FblI GTMKAC 3 cut(s) 350, 2905, 5398
FriOI GRGCYC 3 cut(s) 26, 4617, 5353
GlaI GCGC 4 cut(s) 37, 127, 3571, 4160
GsaI CCCAGC 5 cut(s) 1818, 2365, 4665, 4754, 4871
HaeII RGCGCY 3 cut(s) 39, 129, 3573
HapII CCGG 9 cut(s) 11, 33, 55, 113, 145, 2609, 3308, 4920, 5270
HgaI GACGC 1 cut(s) 203
HhaI GCGC 4 cut(s) 38, 128, 3572, 4161
Hin1I GRCGYC 3 cut(s) 36, 240, 5136
Hin6I GCGC 4 cut(s) 36, 126, 3570, 4159
HinP1I GCGC 4 cut(s) 36, 126, 3570, 4159
HincII GTYRAC 2 cut(s) 3631, 5399
HindII GTYRAC 2 cut(s) 3631, 5399
HindIII AAGCTT 4 cut(s) 1285, 1689, 3912, 4463
HpaII CCGG 9 cut(s) 11, 33, 55, 113, 145, 2609, 3308, 4920, 5270
HphI GGTGA 4 cut(s) 1445, 1831, 2633, 2900
Hpy99I CGWCG 2 cut(s) 197, 1125
HpyCH4IV ACGT 7 cut(s) 240, 1123, 1843, 2629, 4345, 5136, 5266
HpySE526I ACGT 7 cut(s) 240, 1123, 1843, 2629, 4345, 5136, 5266
Hsp92I GRCGYC 3 cut(s) 36, 240, 5136
HspAI GCGC 4 cut(s) 36, 126, 3570, 4159
KasI GGCGCC 1 cut(s) 35
Kpn2I TCCGGA 1 cut(s) 2608
Ksp22I TGATCA 4 cut(s) 1344, 1488, 1960, 4873
LguI GCTCTTC 1 cut(s) 1461
LmnI GCTCC 5 cut(s) 35, 91, 168, 821, 4762
MaeII ACGT 7 cut(s) 240, 1123, 1843, 2629, 4345, 5136, 5266
MbiI CCGCTC 1 cut(s) 2582
MfeI CAATTG 6 cut(s) 877, 1335, 3720, 3956, 4509, 4803
MflI RGATCY 4 cut(s) 580, 2911, 3289, 4216
MhlI GDGCHC 8 cut(s) 26, 886, 1208, 3250, 3307, 4617, 4720, 5353
MlsI TGGCCA 1 cut(s) 5385
MluNI TGGCCA 1 cut(s) 5385
Mly113I GGCGCC 1 cut(s) 36
MlyI GAGTC 5 cut(s) 172, 899, 1889, 3367, 4455
MmeI TCCRAC 6 cut(s) 146, 319, 2275, 3317, 3822, 5105
Mox20I TGGCCA 1 cut(s) 5385
Mph1103I ATGCAT 1 cut(s) 3277
MroI TCCGGA 1 cut(s) 2608
MroXI GAANNNNTTC 4 cut(s) 342, 969, 2488, 4119
MscI TGGCCA 1 cut(s) 5385
MslI CAYNNNNRTG 2 cut(s) 1521, 2727
Msp20I TGGCCA 1 cut(s) 5385
MspA1I CMGCKG 7 cut(s) 943, 1408, 1663, 2291, 2642, 4754, 4871
MspCI CTTAAG 1 cut(s) 4136
MspI CCGG 9 cut(s) 11, 33, 55, 113, 145, 2609, 3308, 4920, 5270
MunI CAATTG 6 cut(s) 877, 1335, 3720, 3956, 4509, 4803
Mva1269I GAATGC 6 cut(s) 720, 1816, 3499, 3678, 5124, 5212
MvnI CGCG 1 cut(s) 1808
NarI GGCGCC 1 cut(s) 36
NciI CCSGG 1 cut(s) 4920
NdeI CATATG 2 cut(s) 762, 3256
NlaIV GGNNCC 8 cut(s) 31, 37, 768, 1870, 3291, 4749, 5066, 5274
NmeAIII GCCGAG 1 cut(s) 186
NmuCI GTSAC 7 cut(s) 1637, 1892, 2160, 2621, 2888, 3334, 5141
NruI TCGCGA 1 cut(s) 1808
NsiI ATGCAT 1 cut(s) 3277
NspI RCATGY 3 cut(s) 1635, 3275, 3928
NspV TTCGAA 1 cut(s) 2791
OliI CACNNNNGTG 1 cut(s) 1521
PaeI GCATGC 1 cut(s) 3275
PaqCI CACCTGC 1 cut(s) 2532
PceI AGGCCT 2 cut(s) 1611, 3829
PciI ACATGT 1 cut(s) 3924
PciSI GCTCTTC 1 cut(s) 1461
PcsI WCGNNNNNNNCGW 1 cut(s) 4477
PctI GAATGC 6 cut(s) 720, 1816, 3499, 3678, 5124, 5212
PdmI GAANNNNTTC 4 cut(s) 342, 969, 2488, 4119
PflMI CCANNNNNTGG 1 cut(s) 301
PfoI TCCNGGA 1 cut(s) 3228
PinAI ACCGGT 1 cut(s) 3307
PleI GAGTC 5 cut(s) 171, 898, 1889, 3366, 4454
PluTI GGCGCC 1 cut(s) 39
PpsI GAGTC 5 cut(s) 171, 898, 1889, 3366, 4454
Ppu21I YACGTR 1 cut(s) 1844
PpuMI RGGWCCY 3 cut(s) 139, 362, 4747
PscI ACATGT 1 cut(s) 3924
Psp124BI GAGCTC 1 cut(s) 26
Psp5II RGGWCCY 3 cut(s) 139, 362, 4747
PspFI CCCAGC 5 cut(s) 1814, 2361, 4661, 4750, 4867
PspN4I GGNNCC 8 cut(s) 31, 37, 768, 1870, 3291, 4749, 5066, 5274
PspPI GGNCC 7 cut(s) 139, 362, 919, 2565, 4539, 4747, 5272
PspPPI RGGWCCY 3 cut(s) 139, 362, 4747
PstNI CAGNNNCTG 1 cut(s) 1321
PsuI RGATCY 4 cut(s) 580, 2911, 3289, 4216
PvuII CAGCTG 6 cut(s) 943, 1408, 2291, 2642, 4754, 4871
RruI TCGCGA 1 cut(s) 1808
RseI CAYNNNNRTG 2 cut(s) 1521, 2727
SacI GAGCTC 1 cut(s) 26
SalI GTCGAC 1 cut(s) 5397
SapI GCTCTTC 1 cut(s) 1461
Sau96I GGNCC 7 cut(s) 139, 362, 919, 2565, 4539, 4747, 5272
SchI GAGTC 5 cut(s) 172, 899, 1889, 3367, 4455
SduI GDGCHC 8 cut(s) 26, 886, 1208, 3250, 3307, 4617, 4720, 5353
SfcI CTRYAG 2 cut(s) 771, 1519
SfoI GGCGCC 1 cut(s) 37
SfuI TTCGAA 1 cut(s) 2791
SinI GGWCC 4 cut(s) 139, 362, 4539, 4747
SmiMI CAYNNNNRTG 2 cut(s) 1521, 2727
SmlI CTYRAG 5 cut(s) 1613, 2240, 3902, 4136, 4431
SmoI CTYRAG 5 cut(s) 1613, 2240, 3902, 4136, 4431
SpeI ACTAGT 1 cut(s) 2855
SphI GCATGC 1 cut(s) 3275
SseBI AGGCCT 2 cut(s) 1611, 3829
SspDI GGCGCC 1 cut(s) 35
SspI AATATT 3 cut(s) 1189, 3405, 4087
SstI GAGCTC 1 cut(s) 26
StuI AGGCCT 2 cut(s) 1611, 3829
StyI CCWWGG 4 cut(s) 46, 2568, 4162, 5276
TaiI ACGT 7 cut(s) 243, 1126, 1846, 2632, 4348, 5139, 5269
TatI WGTACW 4 cut(s) 327, 392, 4566, 4963
TauI GCSGC 4 cut(s) 126, 275, 645, 1958
TseFI GTSAC 7 cut(s) 1637, 1892, 2160, 2621, 2888, 3334, 5141
Tsp45I GTSAC 7 cut(s) 1637, 1892, 2160, 2621, 2888, 3334, 5141
TspGWI ACGGA 4 cut(s) 3671, 3887, 3980, 4589
Van91I CCANNNNNTGG 1 cut(s) 301
Vha464I CTTAAG 1 cut(s) 4136
VneI GTGCAC 5 cut(s) 882, 1204, 3246, 3303, 4716
VpaK11BI GGWCC 4 cut(s) 139, 362, 4539, 4747
XagI CCTNNNNNAGG 2 cut(s) 260, 5280
XceI RCATGY 3 cut(s) 1635, 3275, 3928
XcmI CCANNNNNNNNNTGG 2 cut(s) 2729, 3982
XmaJI CCTAGG 1 cut(s) 5276
XmiI GTMKAC 3 cut(s) 350, 2905, 5398
XmnI GAANNNNTTC 4 cut(s) 342, 969, 2488, 4119
ZraI GACGTC 2 cut(s) 241, 5137
Zsp2I ATGCAT 1 cut(s) 3277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.