Rmu_sc0006218.1_g000028

Sister chromatid cohesion protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006218.1
OG0010273
Physical Location & Seq
Forward (+)
116766 .. 131669
14904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006218.1_g000028.1.cds

Sequence Viewer

Length: 5808 bp
atgccttctacttgtccttgtccacctcctggtttcaggtttcagccctctgatgctgagattattgggttttacttgatcaacaagatactgggcagaccgaacgcttttgatgtcatcagcaccgttgatgtctactccgacagccctgatcagcttccactcgaccctcatcgcacttccccgccatttgcctcacttaacttctcttccaaatctattcctctctccccaaaactcttcacctccctccctctctctctctctctccaacaaaaccctaattccatccgaaatacccctcgatttccgcttatttacaacccctgccactcccctccgccctcccttcatgcccttgcaccgccgttcgtcgcgccctcgccggtgaacctaccgatgagctatccggtcagttcgagctccggcgccggtgccagtcaccaaggcatccggctctccaatactactcactccgaggtcgctccttgcttgcctttgccttctcttccggtgttctgcggcgcttccgatcaggacctccggttgttcgatgagccgagtcggagcagtgcgaggttgaattactacgacgccgctcaggccagtaggattgctgatctgcttcgagctactgacgtctcgtacttgaacctgagggaggatactgcggcggttcaatatggctatgtccaacctatggaacttcacgatgaagttttacagtacaatccagaagcctttcagtatactactccaggtcctattatggggcaagtgcctggcagtagtacagtaccagataagaagccctttgtgccaattatacctataacaagaacttctgaaagagattatggtgcaactcacaataatcagctcaactatactcccccaaatgacatatctatagcatcctccagaaaaccaaaagcaaagagaaaagctagtgatggcgttacttcctcagttgggtcagatcccgttgcaagtcaagaagccactgttggatgcttctgtgagttggtggaggatttttgcggcagagctgaaattttcagtgaggatcgggacgaagcacagtggttatctatgcctctttctgatcttagaatgcttgcaaatgaaataatgtctcttcgcgcaaagaggcttctacatatggttcctgtagatacttttgttagattgttaaggattttagatcatcagatacatggagctgaaggcttgtctataaatgaacagtcagattcaaatggggtttcatcaatcaattgtgcactagagtccattcatgcagctttagcagtaatggcccataaccagatgccaaagcagctgtataaagaagagatcattgaaagaattctggagttctccaggcatcagataatggatgttatgtgtgcttgcgatccatcatatcgtgctttacataggccaagtgaaaatgggacacttgaagttgaggaagacgaaattcttgatgctgaaactggttcagcaagcaagaggcggcgtagtactaagactgttaaagtgcagaaatcatcatataacagggtctctgctgctgtgaataatattcttcagaaaatgtgcacaattcttggtttgctaaaggatttgttgctgatagaaaagttgtcagatagttgtatcttacaactactgaaaacaagctttactacatttatggtggataatatccagcttttgcaactgaaggcaattggtttgatcagtgggatatactattcatacactcaacatcggacttatgttatagatgagttacttcagctgctttggaagttgcctttctcgaagcgagctttgagatcctatcacctacctgatgaagagcaaacgcagattcaaatgatcactgctttgctgattcagttggttcactgtagtgcaaaccttcctgcacctttaaggcaggattcaagtgttaattccatcttagaagtttctgacaatgctgattatccaattaaaggccttgaggcagcacaagcagcatgttgtcacttttggaaaggggtacttcagcggtttgctaatgtcaagaaccaggaagcttcagaattcaaagttatgatggagaatcttgttactgatctgctgacaacattgaatttacctgaatatcccgcttcagcccctattctggaggttctttgtgtcttgttactcgcgaatgctggggtgaaatctaaagatgtagctgcacgtagcatggctatcgatcttctaggaaccattgctgcaaggttgaagcgtgattctctccttcgcagtaggagcaaattttggatattacaggagttggttagtggggatgcggctgatcagacttatccaaaagatgtatgttctgcttgtttagatggcagggttgaaaagactttctttgtatgtcaaggttgtcagagaatgtttcacgcagattgcatgggagtaagagagcatgaagttcctaaccaatcttggcactgtcagatatgtctctgccggaaacaacttcttgttttacaatcttactgcaagtcacaatacaaagatgatggatttaaggatcataaaagttcaggaagaaaaactgaagttactttttcaattactaaacttgagatcgtccaacagttgcttttgaattacctacaagataccgcatcagctgatgatgttcatctctttattcgttggctttatgtctgcttgtggtacaaggatgatccaccaaagtcccagcagaagttcatttactatcttgcaagactgaactcaaaagcaatagtgcgggattctgggactgttttttccttgctgacaagggacttaatcaagcagattaccttagtattgggacgaaatacttcttttgccagaggatttgataagattctacacctgcttctggcaagtctaagggagaattctcctgtaatcagggccaaggctttacgagcggttagtatagttgtagaggctgatccggaggttttaggagacaaacgtgtgcaaccagctgttgaaggaaggttttgtgattccgcaatttctgttagagaagcagcactggaactcgtagggaggcatattgcttcccatcctgatgttggtttgaagtattttgagaaggttgctgagagaatcaaagatacgggtgtgagtgttcgaaagcgatctattaaaatcatacgagatatgtgtgtttcaaatggggacttctctgaatttactagtgcttgcattgctatcatctcccgtattggtgacgacgaatcaagtatacaggatcttgtttgcaagacattttatgagttctggtttgaggaacatactggttcacatactcaattctttggagatgatagctctgttccattggaggtggctaaaaaaactgaacagattgttgagatgttgaggaggatgccaacaccacaccatcttgttaccgtcattaaacgcaacttagcccttgattttttcccacaagcagctaaagctgcgggaatcaatcccgtcttacttgcatcagtacgcaatcggtgtgagctaatgtgcaagtttttactggaaaggatattgcaggtagaagaaatgaatatccaggaagttgagatgtgtgcacttccatatgtgcaagttctgcatgcattttgtgttgtggatccaacattatgtgcaccggtttctaacccttcgcagtttgtggtcactcttcagccttatctaaagagtcaggatgataatcgagtggttgccaagttattggagagcataatattcataattgattctgtcctgcctttagtaagaaagttgccacaaaatgttcttgaagaacttgaacaagacttaaagagcatgattgttcggcattccttcttgtcagttgttcatgcttgcatcaagtgcctctgtgctgtgagtaaagtggcaggaaaaggcgctgctgttgtggagtatctaattcaggttttttacaaacggctggatgctgaggaagttgacaaccagcagaaagcggggcgatctcttttctgtcttggaatgcttatccgttatggcaactctttactgtgttactctgatcaaacaattgatgttgcaagcagtctcggcttatttaaaagatatcttctaatggacgattttttcttaaaggctagatcattgcaggcattgggctttgtgttaattgctaggcctgaatttatgttggaaaaagatattgggaagatattagaggagacattctcttctagctctgatgttcgtctcaagatgcaagctttgcaaaacatgtacgactatcttcttgatgctgaaactcaattgggaacggatacaaccagtaataatgtggctgattattctgtggagggtggcaatgctgtacctgttgctgcgggtgctggagatactaatatatgtggcggaatagttcagttgtattgggataatattctggagagatgtttggactttaatgaacaaattcgcagctctgcccttaagattgttgaagttgtgctgcgccaagggcttgtgcaccctattacttgtgttccattcctcattgcactggaaacagatccactggaggccaattcgacattagcgcatcatttgctgatgaatatgaatgaaaagtacccttcattttttgaaagccgactaggggatggccttcaaatgtcttttggatttatacaatccatacgtccaagtactgaacgtgagaacacaaaaccaccatcaaaggcttctggaaatgcaaagggaaaagttgatgatgtttcttttgctcaagcaagattgggagtctctcgaatctacaagcttattcgtgctaaccgtgtttcaaggaacaaatttatgtcctcaattgttcgcaagtttgataatcctatctggaccacttcagtagtccctttcctcatgtactgcacggaaatacttgccttgctgccattcacaacacctgatgagcccctttatttagtctatgctataaatagagtaatacaagttaaggctgggcaacttgaagcaaagctgaaagctctgacattgcatctgttacaaagaggtgcaccccgtgggaatggcgtaattaaagaggacccagctgctccgccctttacaagaggaatggcatcagtggatttgaatgggacaattgaaccagaatcagcttattacatggcagctatggatttgaatgggacgattgaacaagacccagctgatcagtctgtctcaaatcaagataccgtgttagaggcaaagatgcacggtaaaggttctgtcagttcctctggcatctcaatagatgatgtacagataatccaggcagactgcctggcagctattgcattgcagcttcttttgaaactgaagagacacctaaaaattgtgtatagcttgaatgatgctaggtgccaggctttctctccaaccgatcccattaaacctggagatgctttctccaagcagagcattccttttgacgtcagtgacacacatactaacttgcctggaacttatcaagaattggtacaaagatatcaggaattcaagaatgcgttgaaggatgatacagttgatttctcaacttacacagcaaatctcaaaagaaaacgtccggcccctaggaaaggaaggaaatctggagttagagatgatgaggatgacaatgatgatgatgaagattggtcaggtggggctcggaggctgagttatagtgggaggagaggtggccacagtagaagtcgacaacgatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000070 GO:0000122 GO:0000278 GO:0000280 GO:0000428 GO:0000819 GO:0001654 GO:0001655 GO:0001656 GO:0001822 GO:0003006 GO:0003007 GO:0003008 GO:0003151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007064 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0007423 GO:0007507 GO:0007548 GO:0007600 GO:0007605 GO:0008022 GO:0008104 GO:0008150 GO:0009314 GO:0009628 GO:0009653 GO:0009790 GO:0009887 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010165 GO:0010171 GO:0010212 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010720 GO:0016043 GO:0016591 GO:0019219 GO:0019222 GO:0019899 GO:0019904 GO:0022008 GO:0022402 GO:0022414 GO:0030154 GO:0030326 GO:0030334 GO:0030335 GO:0030880 GO:0031056 GO:0031058 GO:0031063 GO:0031065 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032039 GO:0032116 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0033365 GO:0033554 GO:0034085 GO:0034086 GO:0034088 GO:0034502 GO:0034613 GO:0035107 GO:0035108 GO:0035112 GO:0035113 GO:0035115 GO:0035136 GO:0035239 GO:0035261 GO:0035295 GO:0040007 GO:0040008 GO:0040012 GO:0040017 GO:0042471 GO:0042634 GO:0042826 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043388 GO:0043583 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045597 GO:0045892 GO:0045934 GO:0045995 GO:0047485 GO:0048285 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048546 GO:0048557 GO:0048562 GO:0048565 GO:0048566 GO:0048568 GO:0048589 GO:0048592 GO:0048598 GO:0048608 GO:0048638 GO:0048699 GO:0048731 GO:0048736 GO:0048806 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0050896 GO:0050954 GO:0051094 GO:0051098 GO:0051099 GO:0051101 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051253 GO:0051270 GO:0051272 GO:0051276 GO:0051641 GO:0051716 GO:0051960 GO:0051962 GO:0055029 GO:0055123 GO:0060065 GO:0060173 GO:0060255 GO:0060284 GO:0060322 GO:0060323 GO:0060324 GO:0060325 GO:0061008 GO:0061010 GO:0061038 GO:0061458 GO:0061695 GO:0065007 GO:0065009 GO:0070013 GO:0070087 GO:0070727 GO:0071168 GO:0071214 GO:0071478 GO:0071479 GO:0071481 GO:0071840 GO:0071921 GO:0072001 GO:0072359 GO:0080090 GO:0090311 GO:0090312 GO:0090596 GO:0090694 GO:0098813 GO:0104004 GO:0140014 GO:1902275 GO:1902494 GO:1902679 GO:1903047 GO:1903506 GO:1903507 GO:1905269 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2000145 GO:2000147 GO:2001141 GO:2001222 GO:2001224 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04111 map04111
Pfam Domains
Protein Families
Cohesin_HEAT Nipped-B_C PHD

Protein Analysis

1935

Amino Acids

215.73

Weight (kDa)

6.38

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15540 AT5G15540
fragaria_vesca FvH4_6g47080 FvH4_6g47080 FvH4_6g47080
malus_domestica MD09G1064300.v1.1 MD17G1057600.v1.1
prunus_persica Prupe.3G256700_v2.0.a1
pyrus_communis pycom111g05400 pycom17g05770
rosa_chinensis RchiOBHm_Chr2g0166131
rosa_laevigata RLG00000021588
rosa_multiflora Rmu_sc0006218.1_g000028
rosa_roxburghii Rroxscaffold_2G00085180
rosa_rugosa Rorug02G0520500 Rorug02G0520600
rosa_samantha Rh2AG586600 Rh2BG598400 Rh2CG569100 Rh2DG608300
rosa_wichuraiana Rw2G048900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2931
AatII GACGTC 2 cut(s) 642, 5538
Acc36I ACCTGC 2 cut(s) 2931, 3598
AccB1I GGYRCC 3 cut(s) 428, 434, 5463
AccB7I CCANNNNNTGG 2 cut(s) 29, 700
AccBSI CCGCTC 2 cut(s) 599, 2981
AccI GTMKAC 4 cut(s) 135, 749, 3304, 5797
AccII CGCG 3 cut(s) 377, 1143, 2207
AccIII TCCGGA 1 cut(s) 3007
AcoI YGGCCR 1 cut(s) 5782
AcyI GRCGYC 4 cut(s) 429, 594, 639, 5535
AdeI CACNNNGTG 1 cut(s) 5123
AflII CTTAAG 1 cut(s) 4535
AflIII ACRYGT 2 cut(s) 3028, 4323
AgeI ACCGGT 1 cut(s) 3706
AhlI ACTAGT 1 cut(s) 3254
AjuI GAANNNNNNNTTGG 4 cut(s) 990, 1022, 4236, 4268
AleI CACNNNNGTG 1 cut(s) 1920
Alw21I GWGCWC 7 cut(s) 425, 1285, 1607, 3649, 3706, 4575, 5119
Alw44I GTGCAC 6 cut(s) 1281, 1603, 3645, 3702, 4571, 5115
Aor13HI TCCGGA 1 cut(s) 3007
ApaLI GTGCAC 6 cut(s) 1281, 1603, 3645, 3702, 4571, 5115
AsiGI ACCGGT 1 cut(s) 3706
Asp700I GAANNNNTTC 4 cut(s) 741, 1368, 2887, 4518
AspA2I CCTAGG 1 cut(s) 5675
AspLEI GCGC 7 cut(s) 379, 431, 527, 1145, 3971, 4560, 4645
AspS9I GGNCC 7 cut(s) 538, 761, 1318, 2964, 4938, 5146, 5671
AsuHPI GGTGA 6 cut(s) 235, 400, 434, 1844, 2230, 3299
AsuII TTCGAA 1 cut(s) 3190
AvaII GGWCC 4 cut(s) 538, 761, 4938, 5146
AvrII CCTAGG 1 cut(s) 5675
AxyI CCTNAGG 1 cut(s) 656
BaeGI GKGCMC 6 cut(s) 1285, 1607, 3649, 3706, 4575, 5119
BaeI ACNNNNGTAYC 4 cut(s) 1167, 1200, 3165, 3198
BalI TGGCCA 1 cut(s) 5784
BamHI GGATCC 1 cut(s) 3688
BanI GGYRCC 3 cut(s) 428, 434, 5463
BanII GRGCYC 3 cut(s) 425, 5016, 5752
BarI GAAGNNNNNNTAC 2 cut(s) 2759, 2791
BbsI GAAGAC 1 cut(s) 1482
Bbv12I GWGCWC 7 cut(s) 425, 1285, 1607, 3649, 3706, 4575, 5119
BbvCI CCTCAGC 1 cut(s) 4020
BceAI ACGGC 2 cut(s) 352, 4025
BcgI CGANNNNNNTGC 2 cut(s) 2236, 2270
BciVI GTATCC 2 cut(s) 658, 4360
BclI TGATCA 7 cut(s) 78, 151, 1743, 1887, 2359, 4111, 5272
BcuI ACTAGT 1 cut(s) 3254
BfmI CTRYAG 3 cut(s) 909, 1170, 1918
BfoI RGCGCY 3 cut(s) 432, 528, 3972
BfrI CTTAAG 1 cut(s) 4535
BfuAI ACCTGC 2 cut(s) 2931, 3598
BfuI GTATCC 2 cut(s) 658, 4360
BglI GCCNNNNNGGC 1 cut(s) 602
BlnI CCTAGG 1 cut(s) 5675
BmcAI AGTACT 2 cut(s) 1528, 4753
Bme18I GGWCC 4 cut(s) 538, 761, 4938, 5146
BmgT120I GGNCC 7 cut(s) 538, 761, 1318, 2964, 4938, 5146, 5671
BmiI GGNNCC 8 cut(s) 430, 436, 1167, 2269, 3690, 5148, 5465, 5673
BmrI ACTGGG 1 cut(s) 101
BmuI ACTGGG 1 cut(s) 101
BpiI GAAGAC 1 cut(s) 1482
BplI GAGNNNNNCTC 2 cut(s) 4262, 4294
Bpu10I CCTNAGC 2 cut(s) 600, 4020
Bpu14I TTCGAA 1 cut(s) 3190
BpuEI CTTGAG 4 cut(s) 2033, 2660, 4286, 4815
Bsa29I ATCGAT 1 cut(s) 2256
BsaAI YACGTR 1 cut(s) 2243
BsaBI GATNNNNATC 2 cut(s) 2700, 3768
BsaHI GRCGYC 4 cut(s) 429, 594, 639, 5535
BsaI GGTCTC 1 cut(s) 1573
BsaJI CCNNGG 6 cut(s) 445, 477, 2967, 4561, 5122, 5675
BsaWI WCCGGW 5 cut(s) 409, 511, 543, 3007, 3706
BsaXI ACNNNNNCTCC 4 cut(s) 5746, 5764, 5776, 5794
Bse118I RCCGGY 3 cut(s) 385, 431, 3706
Bse21I CCTNAGG 1 cut(s) 656
Bse3DI GCAATG 7 cut(s) 2271, 3264, 4193, 4417, 4599, 5093, 5399
Bse8I GATNNNNATC 2 cut(s) 2700, 3768
BseAI TCCGGA 1 cut(s) 3007
BseCI ATCGAT 1 cut(s) 2256
BseDI CCNNGG 6 cut(s) 445, 477, 2967, 4561, 5122, 5675
BseJI GATNNNNATC 2 cut(s) 2700, 3768
BseMI GCAATG 7 cut(s) 2271, 3264, 4193, 4417, 4599, 5093, 5399
BseMII CTCAG 7 cut(s) 48, 614, 647, 981, 3150, 4011, 5750
BseRI GAGGAG 3 cut(s) 3457, 4283, 5788
BseSI GKGCMC 6 cut(s) 1285, 1607, 3649, 3706, 4575, 5119
BseYI CCCAGC 5 cut(s) 2213, 2760, 5060, 5149, 5266
BsgI GTGCAG 4 cut(s) 1565, 1920, 2223, 4954
Bsh1236I CGCG 3 cut(s) 377, 1143, 2207
BshNI GGYRCC 3 cut(s) 428, 434, 5463
BshTI ACCGGT 1 cut(s) 3706
BshVI ATCGAT 1 cut(s) 2256
BsiHKAI GWGCWC 7 cut(s) 425, 1285, 1607, 3649, 3706, 4575, 5119
BsmBI CGTCTC 2 cut(s) 646, 4304
BsmI GAATGC 6 cut(s) 1119, 2215, 3898, 4077, 5523, 5611
Bso31I GGTCTC 1 cut(s) 1573
Bsp119I TTCGAA 1 cut(s) 3190
Bsp1286I GDGCHC 9 cut(s) 425, 1285, 1607, 3649, 3706, 4575, 5016, 5119, 5752
Bsp13I TCCGGA 1 cut(s) 3007
Bsp1407I TGTACA 1 cut(s) 5362
Bsp68I TCGCGA 1 cut(s) 2207
BspCNI CTCAG 7 cut(s) 49, 613, 648, 980, 3151, 4012, 5751
BspDI ATCGAT 1 cut(s) 2256
BspEI TCCGGA 1 cut(s) 3007
BspFNI CGCG 3 cut(s) 377, 1143, 2207
BspLI GGNNCC 8 cut(s) 430, 436, 1167, 2269, 3690, 5148, 5465, 5673
BspMI ACCTGC 2 cut(s) 2931, 3598
BspQI GCTCTTC 1 cut(s) 1860
BspT104I TTCGAA 1 cut(s) 3190
BspT107I GGYRCC 3 cut(s) 428, 434, 5463
BspTI CTTAAG 1 cut(s) 4535
BspTNI GGTCTC 1 cut(s) 1573
BsrBI CCGCTC 2 cut(s) 599, 2981
BsrDI GCAATG 7 cut(s) 2271, 3264, 4193, 4417, 4599, 5093, 5399
BsrFI RCCGGY 3 cut(s) 385, 431, 3706
BsrGI TGTACA 1 cut(s) 5362
BssAI RCCGGY 3 cut(s) 385, 431, 3706
BssECI CCNNGG 6 cut(s) 445, 477, 2967, 4561, 5122, 5675
BssNAI GTATAC 2 cut(s) 750, 3305
BssNI GRCGYC 4 cut(s) 429, 594, 639, 5535
BssT1I CCWWGG 4 cut(s) 445, 2967, 4561, 5675
Bst1107I GTATAC 2 cut(s) 750, 3305
Bst6I CTCTTC 9 cut(s) 214, 245, 513, 1143, 1347, 1860, 3744, 4285, 5417
BstACI GRCGYC 4 cut(s) 429, 594, 639, 5535
BstAFI CTTAAG 1 cut(s) 4535
BstAPI GCANNNNNTGC 5 cut(s) 3667, 3933, 4315, 4651, 5396
BstAUI TGTACA 1 cut(s) 5362
BstBAI YACGTR 1 cut(s) 2243
BstBI TTCGAA 1 cut(s) 3190
BstDSI CCRYGG 1 cut(s) 5122
BstENI CCTNNNNNAGG 2 cut(s) 659, 5679
BstFNI CGCG 3 cut(s) 377, 1143, 2207
BstH2I RGCGCY 3 cut(s) 432, 528, 3972
BstHHI GCGC 7 cut(s) 379, 431, 527, 1145, 3971, 4560, 4645
BstNSI RCATGY 3 cut(s) 2034, 3674, 4327
BstSFI CTRYAG 3 cut(s) 909, 1170, 1918
BstSLI GKGCMC 6 cut(s) 1285, 1607, 3649, 3706, 4575, 5119
BstUI CGCG 3 cut(s) 377, 1143, 2207
BstV2I GAAGAC 1 cut(s) 1482
BstX2I RGATCY 5 cut(s) 979, 1844, 3310, 3688, 4615
BstXI CCANNNNNNTGG 1 cut(s) 3790
BstYI RGATCY 5 cut(s) 979, 1844, 3310, 3688, 4615
BstZ17I GTATAC 2 cut(s) 750, 3305
Bsu15I ATCGAT 1 cut(s) 2256
Bsu36I CCTNAGG 1 cut(s) 656
BsuI GTATCC 2 cut(s) 658, 4360
BsuTUI ATCGAT 1 cut(s) 2256
BtgI CCRYGG 1 cut(s) 5122
BtgZI GCGATG 1 cut(s) 158
BtsI GCAGTG 2 cut(s) 577, 1890
BtuMI TCGCGA 1 cut(s) 2207
BveI ACCTGC 2 cut(s) 2931, 3598
CfoI GCGC 7 cut(s) 379, 431, 527, 1145, 3971, 4560, 4645
Cfr10I RCCGGY 3 cut(s) 385, 431, 3706
Cfr13I GGNCC 7 cut(s) 538, 761, 1318, 2964, 4938, 5146, 5671
ClaI ATCGAT 1 cut(s) 2256
CseI GACGC 1 cut(s) 602
CspAI ACCGGT 1 cut(s) 3706
DinI GGCGCC 1 cut(s) 430
DraI TTTAAA 1 cut(s) 4150
DraIII CACNNNGTG 1 cut(s) 5123
EaeI YGGCCR 1 cut(s) 5782
Eam1104I CTCTTC 9 cut(s) 214, 245, 513, 1143, 1347, 1860, 3744, 4285, 5417
EarI CTCTTC 9 cut(s) 214, 245, 513, 1143, 1347, 1860, 3744, 4285, 5417
EciI GGCGGA 3 cut(s) 330, 4473, 5148
Ecl136II GAGCTC 1 cut(s) 423
Eco130I CCWWGG 4 cut(s) 445, 2967, 4561, 5675
Eco147I AGGCCT 2 cut(s) 2010, 4228
Eco24I GRGCYC 3 cut(s) 425, 5016, 5752
Eco31I GGTCTC 1 cut(s) 1573
Eco32I GATATC 2 cut(s) 4157, 5591
Eco47I GGWCC 4 cut(s) 538, 761, 4938, 5146
Eco53kI GAGCTC 1 cut(s) 423
Eco81I CCTNAGG 1 cut(s) 656
EcoICRI GAGCTC 1 cut(s) 423
EcoNI CCTNNNNNAGG 2 cut(s) 659, 5679
EcoO109I RGGNCCY 3 cut(s) 538, 761, 5146
EcoRI GAATTC 4 cut(s) 1368, 2096, 2947, 5597
EcoRV GATATC 2 cut(s) 4157, 5591
EcoT14I CCWWGG 4 cut(s) 445, 2967, 4561, 5675
EcoT22I ATGCAT 1 cut(s) 3676
EcoT38I GRGCYC 3 cut(s) 425, 5016, 5752
EgeI GGCGCC 1 cut(s) 430
EheI GGCGCC 1 cut(s) 430
ErhI CCWWGG 4 cut(s) 445, 2967, 4561, 5675
Esp3I CGTCTC 2 cut(s) 646, 4304
FalI AAGNNNNNCTT 6 cut(s) 797, 829, 2040, 2072, 2405, 2437
FauI CCCGC 6 cut(s) 192, 2170, 2805, 3520, 4039, 4423
FauNDI CATATG 2 cut(s) 1161, 3655
FbaI TGATCA 7 cut(s) 78, 151, 1743, 1887, 2359, 4111, 5272
FblI GTMKAC 4 cut(s) 135, 749, 3304, 5797
FriOI GRGCYC 3 cut(s) 425, 5016, 5752
GlaI GCGC 7 cut(s) 378, 430, 526, 1144, 3970, 4559, 4644
GsaI CCCAGC 5 cut(s) 2217, 2764, 5064, 5153, 5270
HaeII RGCGCY 3 cut(s) 432, 528, 3972
HgaI GACGC 1 cut(s) 602
HhaI GCGC 7 cut(s) 379, 431, 527, 1145, 3971, 4560, 4645
Hin1I GRCGYC 4 cut(s) 429, 594, 639, 5535
Hin6I GCGC 7 cut(s) 377, 429, 525, 1143, 3969, 4558, 4643
HinP1I GCGC 7 cut(s) 377, 429, 525, 1143, 3969, 4558, 4643
HincII GTYRAC 2 cut(s) 4030, 5798
HindII GTYRAC 2 cut(s) 4030, 5798
HindIII AAGCTT 4 cut(s) 1684, 2088, 4311, 4862
HphI GGTGA 6 cut(s) 235, 400, 434, 1844, 2230, 3299
Hpy99I CGWCG 3 cut(s) 377, 596, 3296
HpyCH4IV ACGT 7 cut(s) 639, 2242, 3028, 4744, 4759, 5535, 5665
HpySE526I ACGT 7 cut(s) 639, 2242, 3028, 4744, 4759, 5535, 5665
Hsp92I GRCGYC 4 cut(s) 429, 594, 639, 5535
HspAI GCGC 7 cut(s) 377, 429, 525, 1143, 3969, 4558, 4643
KasI GGCGCC 1 cut(s) 428
Kpn2I TCCGGA 1 cut(s) 3007
Ksp22I TGATCA 7 cut(s) 78, 151, 1743, 1887, 2359, 4111, 5272
LguI GCTCTTC 1 cut(s) 1860
LmnI GCTCC 6 cut(s) 428, 490, 567, 1220, 2313, 5161
MaeII ACGT 7 cut(s) 639, 2242, 3028, 4744, 4759, 5535, 5665
MbiI CCGCTC 2 cut(s) 599, 2981
MfeI CAATTG 6 cut(s) 1276, 1734, 4119, 4355, 4908, 5202
MflI RGATCY 5 cut(s) 979, 1844, 3310, 3688, 4615
MhlI GDGCHC 9 cut(s) 425, 1285, 1607, 3649, 3706, 4575, 5016, 5119, 5752
MlsI TGGCCA 1 cut(s) 5784
MluNI TGGCCA 1 cut(s) 5784
Mly113I GGCGCC 1 cut(s) 429
MlyI GAGTC 4 cut(s) 571, 1298, 3766, 4854
MmeI TCCRAC 9 cut(s) 165, 295, 545, 718, 988, 2674, 3716, 4221, 5504
Mox20I TGGCCA 1 cut(s) 5784
Mph1103I ATGCAT 1 cut(s) 3676
MroI TCCGGA 1 cut(s) 3007
MroXI GAANNNNTTC 4 cut(s) 741, 1368, 2887, 4518
MscI TGGCCA 1 cut(s) 5784
MslI CAYNNNNRTG 2 cut(s) 1920, 3126
Msp20I TGGCCA 1 cut(s) 5784
MspA1I CMGCKG 7 cut(s) 1342, 1807, 2062, 2690, 3041, 5153, 5270
MspCI CTTAAG 1 cut(s) 4535
MunI CAATTG 6 cut(s) 1276, 1734, 4119, 4355, 4908, 5202
Mva1269I GAATGC 6 cut(s) 1119, 2215, 3898, 4077, 5523, 5611
MvnI CGCG 3 cut(s) 377, 1143, 2207
NarI GGCGCC 1 cut(s) 429
NdeI CATATG 2 cut(s) 1161, 3655
NlaIV GGNNCC 8 cut(s) 430, 436, 1167, 2269, 3690, 5148, 5465, 5673
NmeAIII GCCGAG 2 cut(s) 585, 4119
NmuCI GTSAC 6 cut(s) 440, 2036, 2559, 3287, 3733, 5540
NruI TCGCGA 1 cut(s) 2207
NsiI ATGCAT 1 cut(s) 3676
NspI RCATGY 3 cut(s) 2034, 3674, 4327
NspV TTCGAA 1 cut(s) 3190
OliI CACNNNNGTG 1 cut(s) 1920
PaeI GCATGC 1 cut(s) 3674
PaqCI CACCTGC 1 cut(s) 2931
PceI AGGCCT 2 cut(s) 2010, 4228
PciI ACATGT 1 cut(s) 4323
PciSI GCTCTTC 1 cut(s) 1860
PcsI WCGNNNNNNNCGW 1 cut(s) 4876
PctI GAATGC 6 cut(s) 1119, 2215, 3898, 4077, 5523, 5611
PdmI GAANNNNTTC 4 cut(s) 741, 1368, 2887, 4518
PflMI CCANNNNNTGG 2 cut(s) 29, 700
PfoI TCCNGGA 1 cut(s) 3627
PinAI ACCGGT 1 cut(s) 3706
PleI GAGTC 4 cut(s) 570, 1297, 3765, 4853
PluTI GGCGCC 1 cut(s) 432
PpsI GAGTC 4 cut(s) 570, 1297, 3765, 4853
Ppu21I YACGTR 1 cut(s) 2243
PpuMI RGGWCCY 3 cut(s) 538, 761, 5146
PscI ACATGT 1 cut(s) 4323
Psp124BI GAGCTC 1 cut(s) 425
Psp5II RGGWCCY 3 cut(s) 538, 761, 5146
PspFI CCCAGC 5 cut(s) 2213, 2760, 5060, 5149, 5266
PspN4I GGNNCC 8 cut(s) 430, 436, 1167, 2269, 3690, 5148, 5465, 5673
PspPI GGNCC 7 cut(s) 538, 761, 1318, 2964, 4938, 5146, 5671
PspPPI RGGWCCY 3 cut(s) 538, 761, 5146
PsuI RGATCY 5 cut(s) 979, 1844, 3310, 3688, 4615
PvuII CAGCTG 6 cut(s) 1342, 1807, 2690, 3041, 5153, 5270
RruI TCGCGA 1 cut(s) 2207
RseI CAYNNNNRTG 2 cut(s) 1920, 3126
SacI GAGCTC 1 cut(s) 425
SalI GTCGAC 1 cut(s) 5796
SapI GCTCTTC 1 cut(s) 1860
Sau96I GGNCC 7 cut(s) 538, 761, 1318, 2964, 4938, 5146, 5671
ScaI AGTACT 2 cut(s) 1528, 4753
SchI GAGTC 4 cut(s) 571, 1298, 3766, 4854
SduI GDGCHC 9 cut(s) 425, 1285, 1607, 3649, 3706, 4575, 5016, 5119, 5752
SfcI CTRYAG 3 cut(s) 909, 1170, 1918
SfoI GGCGCC 1 cut(s) 430
SfuI TTCGAA 1 cut(s) 3190
SgrAI CRCCGGYG 2 cut(s) 385, 431
SinI GGWCC 4 cut(s) 538, 761, 4938, 5146
SmiMI CAYNNNNRTG 2 cut(s) 1920, 3126
SmlI CTYRAG 5 cut(s) 2012, 2639, 4301, 4535, 4830
SmoI CTYRAG 5 cut(s) 2012, 2639, 4301, 4535, 4830
SpeI ACTAGT 1 cut(s) 3254
SphI GCATGC 1 cut(s) 3674
SseBI AGGCCT 2 cut(s) 2010, 4228
SspDI GGCGCC 1 cut(s) 428
SspI AATATT 3 cut(s) 1588, 3804, 4486
SstI GAGCTC 1 cut(s) 425
StuI AGGCCT 2 cut(s) 2010, 4228
StyI CCWWGG 4 cut(s) 445, 2967, 4561, 5675
TaiI ACGT 7 cut(s) 642, 2245, 3031, 4747, 4762, 5538, 5668
TaqII GACCGA 1 cut(s) 115
TatI WGTACW 6 cut(s) 726, 791, 1526, 4751, 4965, 5362
TauI GCSGC 6 cut(s) 525, 599, 674, 1044, 1522, 2357
TseFI GTSAC 6 cut(s) 440, 2036, 2559, 3287, 3733, 5540
Tsp45I GTSAC 6 cut(s) 440, 2036, 2559, 3287, 3733, 5540
TspGWI ACGGA 3 cut(s) 4070, 4379, 4988
Van91I CCANNNNNTGG 2 cut(s) 29, 700
Vha464I CTTAAG 1 cut(s) 4535
VneI GTGCAC 6 cut(s) 1281, 1603, 3645, 3702, 4571, 5115
VpaK11BI GGWCC 4 cut(s) 538, 761, 4938, 5146
XagI CCTNNNNNAGG 2 cut(s) 659, 5679
XceI RCATGY 3 cut(s) 2034, 3674, 4327
XcmI CCANNNNNNNNNTGG 2 cut(s) 3128, 4381
XmaJI CCTAGG 1 cut(s) 5675
XmiI GTMKAC 4 cut(s) 135, 749, 3304, 5797
XmnI GAANNNNTTC 4 cut(s) 741, 1368, 2887, 4518
ZraI GACGTC 2 cut(s) 640, 5536
ZrmI AGTACT 2 cut(s) 1528, 4753
Zsp2I ATGCAT 1 cut(s) 3676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.