pycom111g05400

Sister chromatid cohesion protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0010273
Physical Location & Seq
Reverse (-)
3457021 .. 3464774
7754 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g05400.10

Sequence Viewer

Length: 4422 bp
ATGTTATGTGCGCTTATCGATCCATCATATCGTGCTTTGCATAGGCCAAGTCAAAATGGGTCGCTCGAAGTTGAGGAAGATGAAGAGCCTGATGCTGAATTTGGTTCAGCAAGCAAGAAGAGACGCAGTATCAAGACTGTTAAAGTGCAGAAATCCTCATTTAACAGGGTCTCTGCTGCTGTGAATAATATTCTTCAGAAAATGTGCACCATTCTTGGTTTACTCAAGGATTTGTTGTTAATAGAAAGGTTGTCAGATGGTTGTATCTTACAACTAGTGAAAACAAGCTTTACAACATTTATGGTGGATAATATCCAGCTCTTGCAACTGAAGGCAATGGGTTTAATCAGTGCGATATTCTATTCATACACTCAGCATCGCACATATGTGATAGATGAATTAATTCAGTTGCTTTGGAAGTTGCCAGTCTCGAAGCGAGCTTTGAGAGCCTATCACCTACCTGACGAAGAGCAACGGCAGATTCAGATGATCACTGCTTTGCTGATTCAGTTGGTTCACTATAGTGCAAACCTTCCTCAATCATTAAGACAGGAATCAAGTGGTAATTCCATCTTAGAACTTTCTGTCAATGCCGATTATCCAACTAAAGGTCATGAGGCAGCAACAGAGGCATGTTGTCACTTTTGGACTCGTGTACTTCAGCGCTTTGCTAGTGCGAAGGCTCAGGAAGCTTCAGAGTTGAAAGTAATGATGGAGAATCTTGTTACTGATCTCTTGACAACATTGAATTTACCGGAATATCCCGCTTCAGCTCCTATTCTGGAGGTTCTTTGTGTCTTGTTACTCCAGAATGCTGGGCTGAAATCTAAAGACATAGCTGCACGTACCATGGCTATTGACCTTCTCGGCACAATTGCTGCAAGGTTGAAGCGTGATTCTGTACTTTGCAGTAGTGACAAAATCTGGATACTTCAGGAGTTGGTTAGTGACGATGGGGTTGATCAGACATATCCAAGAGATGCATGCTCAGTTTGTTTAGATGGTAGGGTTGAAAAAAATGTCTTTGTATGTCAAGGTTGTCAGAGAATGTTTCATGCTGATTGCATGGGAGTGAGAGAATATGAAGTTCCAAATCGAAGTTGGCACTGTCAGATATGCCTCTGCAGGAAGCAACTTCTTGTTTTACAATCCTATTGTAAGTCACAATACAAAGATGAAGGAACCAAGGATCGAAGTCGTTCAGGAAGAAACACTGCAGTTGCTTTTTCAATTACAAAACCTGAGGTTGTCCAACAGATGCTTTTGAATTACCTTCAAGATACCACATCAGCTGATGATGGGCATCTCTTTGTTCGCTGGTTTTATCTCTTGTTGTGGTACAAGGATGATTCAAAATCACAGCAGAAGCTCATGTACTATCTTGCTCGACTGAAATCAAAAGAAATAGTTCGGGATTCTGGGACTGTTTTTTCATTATTGACAAGGGATTCAGTCAAGAAAATTACTTTAGCATTGGGGCAAAAAAACTCTTTCTCCAGAGGGTTTGATAAGATTCTTCATTTGCTTCTGGCAAGCTTAATGGAGAATTCTCCTGTAATCAGGGCCAAGGCTTTACGAGCGGTCAGTATAATTGTAGAAGCTGATCCGCAGGTATTAGGAGATAAACGAGTTCAATCAGCTGTTGAAGGAAGATTTTGTGATTCTGCAATTTCTGCAAGAGAAGCAGCACTGGAACTTGTAGGAAGGCATATTGCTTCTCATCCTGATGTTGGTTTGCAGTATTTTGAAAAGGTTGCTGAGAGAATTAAAGATACAGGAGTTAGTGTTCGAAAGCGATCCATCAAAATCATACGAGATATGTGCATTTCAAATGCAAACTTCTCCGAATTCACAAGTGCCTGCATTGCTATCATCTCCCGTATTAGTGATGATGAATCAAGTATACAGGATATTGTTTGCAAGACATTTTATGAGTTCTGGTTTGAGGAACCTGCTGGTTCACAAACTCATTTCTTTGGAGATGGTAGCTCTGTTACATTGGAGGTGGCTAAAAAAACTGAACAGATCGTTGAGATGTTGAGGAGGATGCCAAGTCATCAACCTCTTGTGACAGTCATTAAACGCAACTTGGCCCTTGATTTTTTTCCACAATCGGCTAAAGCTGTTGGGATCAACCCTGTCTTACTTGCATCTGTACGCAACCGCTGTGAGTTAATGTGCAAGTGCTTACTAGAAAAGATATTGCAGGTAGAGGAAATGAATATCCAGGAGGTAGATAGGCAGGCACTTCCATATGTACTAGCTTTGCATGCATTCTGTGTTGTGGATCCAACACTTTGTGCACCGGCTTCTGACCCTTCACAGTTTGTGGTCACTCTTCAGCCTTATCTAAAGAGTCAGGCTGATAATCGAGTGGTTGCACAGTTAGTGGAGAGTATAATCTTTATAATTGATGCTGTTCTGCCTTTGGTTAGAAAGTTACCACGAACTGTTGTGGAAGAACTTGAACAAGACTTAAAGAACATGATTGTTCGGCATTCCTTCTTGACTGTTGTGCATGCTTGCATAAAGTGCCTCTGTGCTGTGAGTAAAGTGGCAGGAAAAGGTGCTGCTATAGTGGAGAATCTTATCCAGGTGTTCTTCAAACGTTTGGACGCTCAGGCAGTAGATAACAAGCAGCAAGTGGGGCGGTCTCTTTTCTGTCTCGGATTGCTTATCCGTTATGGCAACTCTTTGCCCAGTAACTCCAACAGAAAAATTGATGTTGTTAGCAGTCTCAGCTTGTTTAAAAAATATCTTCTAGTGGATGATTTTGTAATAAAGGTTCGGTCATTACAGGCAATGGGCTTTGTTCTAATAGCTAGGCCCGAATATATGTTGGAAAAAGATATTGGGAAGATAGTAGAGGCAACATTCTCTTCTAGCTCTGACGTTCGTCTTAGGATGCAAGCATTGCAAAACATGTACGACTATCTTCTTGATGCTGAAAGTAAAATGGGAACAGATGCAGCTAGTGATAATGTGATTCATTGTAATGTGGAAGGCGGCAATGCTGTATCTGTTGCTGCGGGTGCCGGAGATACTAATATCTGTGGTGGTATAGTTCAGTTGTATTGGGATAATATGTTGGTGAGATGTTTAGACCTTAATGAACAAGTTCGCCAATCTGCAGTTAAGATTGTTGAAGTTGTGCTGCGCCAAGGGCTCGTACATCCTATTACATGTGTTCCATACCTTATTGCACTCGAAACAGATCGACTGGAGGCCAATTCGAAGTTAGCTCATCATTTGCTGATGCAAATGAATGAAAAGTACCCAGCATTTTTCGAAAGCCGGCTGGGAGATGGCCTTCAAATGTCTTTTAGTTTCATACAGTCCATAAGTACTAGTTCTGAACACGAGAACAAAAAAATTCAAGCAAAGGCTTCTGGAAATGCGAAGGGAAAATCTGATAATGTTTCTCTCGCACAAGCAAGACTAGGGGTTTCTCGAATCTACAAGCTTATTCGTGCTAACCGGACTTCAAGAAACAAATTTATGTCTTCAATTGTTCGCAAGTTTGATAATACTAGCTGGACACCTTCAGTGGTCCCTTTCCTAATGTACTGCACAGAGATACTTGCCTTGCTACCCTTCACAACACCTGATGAGCCACTTTACTTGGTCTATTCTATAAATAGAGTAGTACAAGTGAGGGCTGGGGCGCTCGAAGCAAAACTGAAAGCTCTGACGTTGCATTTGTTACAAAGAAGTGCACCTCATGGGAACGGCATAATTGAAGATGGTTCAGCTGCTCAACCTTTTACGAGAGGAATGGCGTCATTGGATTTGAATGGAACGGTTCAACAAGAACCAGTGTTTCAACCAGTTACAAGTTATATGTCAACGGAGTGGAATGGTATAATACAACAAGAGCCAGCAGATCAGTCTGTCTCAAATCAAGCTACCCCGTTTGAGGCAAACATGCACGTTACGGGCTCCAACAGTTCCGGTGGCTTCTCAATAGATGACGTAAAGAAATTCCAGGCAGACTGCCTGGCTGCTATTGCGGTGCAGCTTCTTTTGAAATTAAAGAGACACCTAAAAATTGTGTACAGCCTGAACGATGCAAGGTGCCAGGCTTTTTCTCCGGGCGAACCCCTTAAACCGGGAGATGTACTCTCCAGGCAGAACATTCCTTTCGACCTCAGTGATACCCGCACCAATTTGCCAACAACTTATCAAGAATTGGTTCAAAGATATCAGGAATTCAAGAATGCGTTGAGGGAGGATACGATTGATTTCTCAACTTACACTGCAAACATCAAGAGAAAACGGCCAGCCCCCAGAAAAGGGCGGAAATCTGTGGGGGGAGATGACGAGGGCGATGATGACGATGAGGATTATTCAGGCGGGGCTCGGAGGCCGAGTAATATTGGAAGGAGAGGTGGCTACGGTATTAGAAGTCGACAGCGGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000070 GO:0000122 GO:0000278 GO:0000280 GO:0000428 GO:0000819 GO:0001654 GO:0001655 GO:0001656 GO:0001822 GO:0003006 GO:0003007 GO:0003008 GO:0003151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007064 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0007423 GO:0007507 GO:0007548 GO:0007600 GO:0007605 GO:0008022 GO:0008104 GO:0008150 GO:0009314 GO:0009628 GO:0009653 GO:0009790 GO:0009887 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010165 GO:0010171 GO:0010212 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010720 GO:0016043 GO:0016591 GO:0019219 GO:0019222 GO:0019899 GO:0019904 GO:0022008 GO:0022402 GO:0022414 GO:0030154 GO:0030326 GO:0030334 GO:0030335 GO:0030880 GO:0031056 GO:0031058 GO:0031063 GO:0031065 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032039 GO:0032116 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0033365 GO:0033554 GO:0034085 GO:0034086 GO:0034088 GO:0034502 GO:0034613 GO:0035107 GO:0035108 GO:0035112 GO:0035113 GO:0035115 GO:0035136 GO:0035239 GO:0035261 GO:0035295 GO:0040007 GO:0040008 GO:0040012 GO:0040017 GO:0042471 GO:0042634 GO:0042826 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043388 GO:0043583 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045597 GO:0045892 GO:0045934 GO:0045995 GO:0047485 GO:0048285 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048546 GO:0048557 GO:0048562 GO:0048565 GO:0048566 GO:0048568 GO:0048589 GO:0048592 GO:0048598 GO:0048608 GO:0048638 GO:0048699 GO:0048731 GO:0048736 GO:0048806 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0050896 GO:0050954 GO:0051094 GO:0051098 GO:0051099 GO:0051101 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051253 GO:0051270 GO:0051272 GO:0051276 GO:0051641 GO:0051716 GO:0051960 GO:0051962 GO:0055029 GO:0055123 GO:0060065 GO:0060173 GO:0060255 GO:0060284 GO:0060322 GO:0060323 GO:0060324 GO:0060325 GO:0061008 GO:0061010 GO:0061038 GO:0061458 GO:0061695 GO:0065007 GO:0065009 GO:0070013 GO:0070087 GO:0070727 GO:0071168 GO:0071214 GO:0071478 GO:0071479 GO:0071481 GO:0071840 GO:0071921 GO:0072001 GO:0072359 GO:0080090 GO:0090311 GO:0090312 GO:0090596 GO:0090694 GO:0098813 GO:0104004 GO:0140014 GO:1902275 GO:1902494 GO:1902679 GO:1903047 GO:1903506 GO:1903507 GO:1905269 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2000145 GO:2000147 GO:2001141 GO:2001222 GO:2001224 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04111 map04111
Pfam Domains
Protein Families
Cohesin_HEAT Nipped-B_C PHD

Protein Analysis

1474

Amino Acids

164.72

Weight (kDa)

8.48

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15540 AT5G15540
fragaria_vesca FvH4_6g47080 FvH4_6g47080 FvH4_6g47080
malus_domestica MD09G1064300.v1.1 MD17G1057600.v1.1
prunus_persica Prupe.3G256700_v2.0.a1
pyrus_communis pycom111g05400 pycom17g05770
rosa_chinensis RchiOBHm_Chr2g0166131
rosa_laevigata RLG00000021588
rosa_multiflora Rmu_sc0006218.1_g000028
rosa_roxburghii Rroxscaffold_2G00085180
rosa_rugosa Rorug02G0520500 Rorug02G0520600
rosa_samantha Rh2AG586600 Rh2BG598400 Rh2CG569100 Rh2DG608300
rosa_wichuraiana Rw2G048900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2408
Acc36I ACCTGC 3 cut(s) 1600, 1960, 2197
AccB1I GGYRCC 2 cut(s) 3032, 4074
AccBSI CCGCTC 1 cut(s) 1580
AccI GTMKAC 2 cut(s) 1903, 4408
AclI AACGTT 1 cut(s) 2608
AclWI GGATC 7 cut(s) 14, 1197, 1598, 1791, 2138, 2282, 2295
AcoI YGGCCR 1 cut(s) 4277
AcsI RAATTY 8 cut(s) 98, 748, 1546, 1847, 3372, 3494, 3980, 4208
AcuI CTGAAG 8 cut(s) 179, 350, 644, 678, 753, 917, 2324, 3528
AcyI GRCGYC 1 cut(s) 3779
AdeI CACNNNGTG 1 cut(s) 2300
AfeI AGCGCT 1 cut(s) 665
AfiI CCNNNNNNNGG 6 cut(s) 608, 1730, 3916, 4108, 4292, 4293
AflIII ACRYGT 2 cut(s) 2922, 3182
AhlI ACTAGT 2 cut(s) 274, 3347
AjnI CCWGG 6 cut(s) 2226, 2592, 3984, 3996, 4077, 4124
AjuI GAANNNNNNNTTGG 2 cut(s) 2835, 2867
AleI CACNNNNGTG 2 cut(s) 386, 522
AloI GAACNNNNNNTCC 2 cut(s) 1770, 1802
Alw21I GWGCWC 3 cut(s) 209, 2305, 3718
Alw26I GTCTC 8 cut(s) 115, 175, 433, 2658, 2669, 2741, 3898, 4030
Alw44I GTGCAC 3 cut(s) 205, 2301, 3714
AlwI GGATC 7 cut(s) 14, 1197, 1598, 1791, 2138, 2282, 2295
Aor51HI AGCGCT 1 cut(s) 665
AoxI GGCC 8 cut(s) 44, 1563, 2091, 2825, 3225, 3307, 4277, 4364
ApaLI GTGCAC 3 cut(s) 205, 2301, 3714
ApoI RAATTY 8 cut(s) 98, 748, 1546, 1847, 3372, 3494, 3980, 4208
ArsI GACNNNNNNTTYG 2 cut(s) 3682, 3714
AseI ATTAAT 1 cut(s) 401
Asp700I GAANNNNTTC 5 cut(s) 402, 1198, 1407, 3117, 4191
AspLEI GCGC 4 cut(s) 13, 666, 3159, 3667
AspS9I GGNCC 4 cut(s) 1563, 2092, 2826, 3550
AsuC2I CCSGG 2 cut(s) 4092, 4110
AsuHPI GGTGA 2 cut(s) 446, 3103
AsuII TTCGAA 3 cut(s) 1789, 3233, 3288
AvaII GGWCC 1 cut(s) 3550
AxyI CCTNAGG 1 cut(s) 1242
BaeGI GKGCMC 3 cut(s) 209, 2305, 3718
BaeI ACNNNNGTAYC 2 cut(s) 1764, 1797
BamHI GGATCC 1 cut(s) 2287
BanI GGYRCC 2 cut(s) 3032, 4074
BanII GRGCYC 3 cut(s) 3168, 3941, 4360
BarI GAAGNNNNNNTAC 2 cut(s) 1358, 1390
BauI CACGAG 2 cut(s) 651, 3359
BbsI GAAGAC 1 cut(s) 3495
Bbv12I GWGCWC 3 cut(s) 209, 2305, 3718
BceAI ACGGC 3 cut(s) 491, 3745, 4292
BcgI CGANNNNNNTGC 2 cut(s) 1803, 1837
BciT130I CCWGG 6 cut(s) 2228, 2594, 3986, 3998, 4079, 4126
BciVI GTATCC 2 cut(s) 921, 4225
BclI TGATCA 2 cut(s) 489, 961
BcnI CCSGG 2 cut(s) 4092, 4110
BcoDI GTCTC 8 cut(s) 115, 175, 433, 2658, 2669, 2741, 3898, 4030
BcuI ACTAGT 2 cut(s) 274, 3347
BfmI CTRYAG 5 cut(s) 520, 1123, 1215, 2574, 3129
BfoI RGCGCY 2 cut(s) 667, 3668
BfuAI ACCTGC 3 cut(s) 1600, 1960, 2197
BfuI GTATCC 2 cut(s) 921, 4225
BmcAI AGTACT 1 cut(s) 3346
Bme1390I CCNGG 8 cut(s) 2228, 2594, 3986, 3998, 4079, 4092, 4110, 4126
Bme18I GGWCC 1 cut(s) 3550
BmgT120I GGNCC 4 cut(s) 1563, 2092, 2826, 3550
BmiI GGNNCC 7 cut(s) 1183, 1950, 2289, 3034, 3552, 3940, 4076
BmrFI CCNGG 8 cut(s) 2228, 2594, 3986, 3998, 4079, 4092, 4110, 4126
BmrI ACTGGG 1 cut(s) 2694
BmuI ACTGGG 1 cut(s) 2694
BoxI GACNNNNGTC 1 cut(s) 2895
BpiI GAAGAC 1 cut(s) 3495
BplI GAGNNNNNCTC 2 cut(s) 4104, 4136
BpmI CTGGAG 5 cut(s) 791, 803, 1480, 3242, 4108
Bpu10I CCTNAGC 2 cut(s) 684, 2619
Bpu14I TTCGAA 3 cut(s) 1789, 3233, 3288
BpuEI CTTGAG 1 cut(s) 209
BpuMI CCSGG 2 cut(s) 4092, 4110
Bsa29I ATCGAT 1 cut(s) 18
BsaAI YACGTR 1 cut(s) 845
BsaBI GATNNNNATC 1 cut(s) 1302
BsaHI GRCGYC 1 cut(s) 3779
BsaI GGTCTC 2 cut(s) 175, 2658
BsaJI CCNNGG 4 cut(s) 849, 1185, 1566, 3160
BsaWI WCCGGW 3 cut(s) 754, 3477, 3950
BsaXI ACNNNNNCTCC 6 cut(s) 1478, 1508, 1770, 1800, 4354, 4384
Bsc4I CCNNNNNNNGG 6 cut(s) 608, 1730, 3916, 4108, 4292, 4293
Bse118I RCCGGY 2 cut(s) 2305, 3294
Bse1I ACTGG 6 cut(s) 425, 1695, 2700, 3225, 3815, 3827
Bse21I CCTNAGG 1 cut(s) 1242
Bse3DI GCAATG 5 cut(s) 342, 1863, 2808, 2912, 3016
Bse8I GATNNNNATC 1 cut(s) 1302
BseBI CCWGG 6 cut(s) 2228, 2594, 3986, 3998, 4079, 4126
BseCI ATCGAT 1 cut(s) 18
BseDI CCNNGG 4 cut(s) 849, 1185, 1566, 3160
BseGI GGATG 6 cut(s) 1351, 1720, 2052, 2773, 2910, 3172
BseJI GATNNNNATC 1 cut(s) 1302
BseLI CCNNNNNNNGG 6 cut(s) 608, 1730, 3916, 4108, 4292, 4293
BseMI GCAATG 5 cut(s) 342, 1863, 2808, 2912, 3016
BseMII CTCAG 8 cut(s) 386, 698, 1002, 1233, 1749, 2633, 2752, 4162
BseNI ACTGG 6 cut(s) 425, 1695, 2700, 3225, 3815, 3827
BseRI GAGGAG 1 cut(s) 2056
BseSI GKGCMC 3 cut(s) 209, 2305, 3718
BseYI CCCAGC 4 cut(s) 815, 3277, 3298, 3659
BsgI GTGCAG 4 cut(s) 167, 825, 3553, 4034
BshFI GGCC 8 cut(s) 46, 1565, 2093, 2827, 3227, 3309, 4279, 4366
BshNI GGYRCC 2 cut(s) 3032, 4074
BshVI ATCGAT 1 cut(s) 18
BsiHKAI GWGCWC 3 cut(s) 209, 2305, 3718
BsiSI CCGG 8 cut(s) 755, 2306, 3036, 3295, 3478, 3951, 4091, 4109
BslFI GGGAC 2 cut(s) 1435, 3536
BslI CCNNNNNNNGG 6 cut(s) 608, 1730, 3916, 4108, 4292, 4293
BsmAI GTCTC 8 cut(s) 115, 175, 433, 2658, 2669, 2741, 3898, 4030
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 2 cut(s) 1435, 3536
BsmI GAATGC 4 cut(s) 817, 2273, 2497, 4222
BsnI GGCC 8 cut(s) 46, 1565, 2093, 2827, 3227, 3309, 4279, 4366
Bso31I GGTCTC 2 cut(s) 175, 2658
Bsp119I TTCGAA 3 cut(s) 1789, 3233, 3288
Bsp1286I GDGCHC 6 cut(s) 209, 2305, 3168, 3718, 3941, 4360
Bsp1407I TGTACA 1 cut(s) 4053
Bsp19I CCATGG 1 cut(s) 849
BspANI GGCC 8 cut(s) 46, 1565, 2093, 2827, 3227, 3309, 4279, 4366
BspCNI CTCAG 8 cut(s) 385, 697, 1001, 1234, 1750, 2632, 2751, 4161
BspDI ATCGAT 1 cut(s) 18
BspHI TCATGA 1 cut(s) 613
BspLI GGNNCC 7 cut(s) 1183, 1950, 2289, 3034, 3552, 3940, 4076
BspMAI CTGCAG 3 cut(s) 1127, 1219, 3133
BspMI ACCTGC 3 cut(s) 1600, 1960, 2197
BspPI GGATC 7 cut(s) 14, 1197, 1598, 1791, 2138, 2282, 2295
BspQI GCTCTTC 2 cut(s) 78, 462
BspT104I TTCGAA 3 cut(s) 1789, 3233, 3288
BspT107I GGYRCC 2 cut(s) 3032, 4074
BspTNI GGTCTC 2 cut(s) 175, 2658
BsrBI CCGCTC 1 cut(s) 1580
BsrDI GCAATG 5 cut(s) 342, 1863, 2808, 2912, 3016
BsrFI RCCGGY 2 cut(s) 2305, 3294
BsrGI TGTACA 1 cut(s) 4053
BsrI ACTGG 6 cut(s) 425, 1695, 2700, 3225, 3815, 3827
BssAI RCCGGY 2 cut(s) 2305, 3294
BssECI CCNNGG 4 cut(s) 849, 1185, 1566, 3160
BssNAI GTATAC 1 cut(s) 1904
BssNI GRCGYC 1 cut(s) 3779
BssSI CACGAG 2 cut(s) 651, 3359
BssT1I CCWWGG 4 cut(s) 849, 1185, 1566, 3160
Bst1107I GTATAC 1 cut(s) 1904
Bst2BI CACGAG 2 cut(s) 651, 3359
Bst2UI CCWGG 6 cut(s) 2228, 2594, 3986, 3998, 4079, 4126
Bst6I CTCTTC 5 cut(s) 78, 113, 462, 2343, 2884
BstACI GRCGYC 1 cut(s) 3779
BstAPI GCANNNNNTGC 3 cut(s) 1673, 2532, 2914
BstAUI TGTACA 1 cut(s) 4053
BstBAI YACGTR 1 cut(s) 845
BstBI TTCGAA 3 cut(s) 1789, 3233, 3288
BstDSI CCRYGG 1 cut(s) 849
BstF5I GGATG 6 cut(s) 1351, 1720, 2052, 2773, 2910, 3172
BstH2I RGCGCY 2 cut(s) 667, 3668
BstHHI GCGC 4 cut(s) 13, 666, 3159, 3667
BstMAI GTCTC 8 cut(s) 115, 175, 433, 2658, 2669, 2741, 3898, 4030
BstNI CCWGG 6 cut(s) 2228, 2594, 3986, 3998, 4079, 4126
BstNSI RCATGY 7 cut(s) 636, 987, 2273, 2522, 2926, 3186, 3928
BstPAI GACNNNNGTC 1 cut(s) 2895
BstSCI CCNGG 8 cut(s) 2226, 2592, 3984, 3996, 4077, 4090, 4108, 4124
BstSFI CTRYAG 5 cut(s) 520, 1123, 1215, 2574, 3129
BstSLI GKGCMC 3 cut(s) 209, 2305, 3718
BstV2I GAAGAC 1 cut(s) 3495
BstX2I RGATCY 1 cut(s) 2287
BstXI CCANNNNNNTGG 1 cut(s) 815
BstYI RGATCY 1 cut(s) 2287
BstZ17I GTATAC 1 cut(s) 1904
Bsu15I ATCGAT 1 cut(s) 18
Bsu36I CCTNAGG 1 cut(s) 1242
BsuI GTATCC 2 cut(s) 921, 4225
BsuRI GGCC 8 cut(s) 46, 1565, 2093, 2827, 3227, 3309, 4279, 4366
BsuTUI ATCGAT 1 cut(s) 18
BtgI CCRYGG 1 cut(s) 849
BtgZI GCGATG 2 cut(s) 362, 4341
BtsCI GGATG 6 cut(s) 1351, 1720, 2052, 2773, 2910, 3172
BtsI GCAGTG 3 cut(s) 492, 1212, 4254
BtsIMutI CAGTG 9 cut(s) 355, 492, 1105, 1212, 1688, 3552, 3822, 4156, 4254
BveI ACCTGC 3 cut(s) 1600, 1960, 2197
CciI TCATGA 1 cut(s) 613
CfoI GCGC 4 cut(s) 13, 666, 3159, 3667
Cfr10I RCCGGY 2 cut(s) 2305, 3294
Cfr13I GGNCC 4 cut(s) 1563, 2092, 2826, 3550
ClaI ATCGAT 1 cut(s) 18
CseI GACGC 3 cut(s) 132, 2624, 3768
CspCI CAANNNNNGTGG 2 cut(s) 3603, 3638
DraI TTTAAA 1 cut(s) 2749
DraIII CACNNNGTG 1 cut(s) 2300
EaeI YGGCCR 1 cut(s) 4277
Eam1104I CTCTTC 5 cut(s) 78, 113, 462, 2343, 2884
EarI CTCTTC 5 cut(s) 78, 113, 462, 2343, 2884
EciI GGCGGA 1 cut(s) 4312
Eco130I CCWWGG 4 cut(s) 849, 1185, 1566, 3160
Eco24I GRGCYC 3 cut(s) 3168, 3941, 4360
Eco31I GGTCTC 2 cut(s) 175, 2658
Eco32I GATATC 1 cut(s) 4202
Eco47I GGWCC 1 cut(s) 3550
Eco47III AGCGCT 1 cut(s) 665
Eco57I CTGAAG 8 cut(s) 179, 350, 644, 678, 753, 917, 2324, 3528
Eco81I CCTNAGG 1 cut(s) 1242
EcoRI GAATTC 3 cut(s) 1546, 1847, 4208
EcoRII CCWGG 6 cut(s) 2226, 2592, 3984, 3996, 4077, 4124
EcoRV GATATC 1 cut(s) 4202
EcoT14I CCWWGG 4 cut(s) 849, 1185, 1566, 3160
EcoT22I ATGCAT 2 cut(s) 985, 2275
EcoT38I GRGCYC 3 cut(s) 3168, 3941, 4360
ErhI CCWWGG 4 cut(s) 849, 1185, 1566, 3160
Esp3I CGTCTC 1 cut(s) 115
FaqI GGGAC 2 cut(s) 1435, 3536
FauI CCCGC 4 cut(s) 772, 3022, 4166, 4346
FauNDI CATATG 2 cut(s) 385, 2254
FbaI TGATCA 2 cut(s) 489, 961
FblI GTMKAC 2 cut(s) 1903, 4408
FokI GGATG 6 cut(s) 1358, 1707, 2059, 2780, 2917, 3159
FriOI GRGCYC 3 cut(s) 3168, 3941, 4360
GlaI GCGC 4 cut(s) 12, 665, 3158, 3666
GsaI CCCAGC 4 cut(s) 819, 3281, 3302, 3663
GsuI CTGGAG 5 cut(s) 791, 803, 1480, 3242, 4108
HaeII RGCGCY 2 cut(s) 667, 3668
HaeIII GGCC 8 cut(s) 46, 1565, 2093, 2827, 3227, 3309, 4279, 4366
HapII CCGG 8 cut(s) 755, 2306, 3036, 3295, 3478, 3951, 4091, 4109
HgaI GACGC 3 cut(s) 132, 2624, 3768
HhaI GCGC 4 cut(s) 13, 666, 3159, 3667
Hin1I GRCGYC 1 cut(s) 3779
Hin6I GCGC 4 cut(s) 11, 664, 3157, 3665
HinP1I GCGC 4 cut(s) 11, 664, 3157, 3665
HincII GTYRAC 2 cut(s) 3846, 4409
HindII GTYRAC 2 cut(s) 3846, 4409
HindIII AAGCTT 4 cut(s) 286, 690, 1534, 3461
HpaII CCGG 8 cut(s) 755, 2306, 3036, 3295, 3478, 3951, 4091, 4109
HphI GGTGA 2 cut(s) 446, 3103
HpyCH4IV ACGT 6 cut(s) 844, 2608, 2892, 3692, 3930, 3972
HpySE526I ACGT 6 cut(s) 844, 2608, 2892, 3692, 3930, 3972
Hsp92I GRCGYC 1 cut(s) 3779
HspAI GCGC 4 cut(s) 11, 664, 3157, 3665
KroI GCCGGC 1 cut(s) 3294
KroNI GCCGGC 1 cut(s) 3296
Ksp22I TGATCA 2 cut(s) 489, 961
LguI GCTCTTC 2 cut(s) 78, 462
LmnI GCTCC 2 cut(s) 778, 3944
MaeII ACGT 6 cut(s) 844, 2608, 2892, 3692, 3930, 3972
MbiI CCGCTC 1 cut(s) 1580
MfeI CAATTG 2 cut(s) 873, 3507
MflI RGATCY 1 cut(s) 2287
MhlI GDGCHC 6 cut(s) 209, 2305, 3168, 3718, 3941, 4360
MlyI GAGTC 2 cut(s) 643, 2365
MmeI TCCRAC 6 cut(s) 626, 1276, 2315, 2733, 2820, 3966
Mph1103I ATGCAT 2 cut(s) 985, 2275
MroNI GCCGGC 1 cut(s) 3294
MroXI GAANNNNTTC 5 cut(s) 402, 1198, 1407, 3117, 4191
MslI CAYNNNNRTG 5 cut(s) 386, 522, 1070, 1725, 2994
MspA1I CMGCKG 5 cut(s) 1292, 1640, 2166, 3752, 4414
MspI CCGG 8 cut(s) 755, 2306, 3036, 3295, 3478, 3951, 4091, 4109
MspR9I CCNGG 8 cut(s) 2228, 2594, 3986, 3998, 4079, 4092, 4110, 4126
MunI CAATTG 2 cut(s) 873, 3507
Mva1269I GAATGC 4 cut(s) 817, 2273, 2497, 4222
MvaI CCWGG 6 cut(s) 2228, 2594, 3986, 3998, 4079, 4126
NaeI GCCGGC 1 cut(s) 3296
NciI CCSGG 2 cut(s) 4092, 4110
NcoI CCATGG 1 cut(s) 849
NdeI CATATG 2 cut(s) 385, 2254
NgoMIV GCCGGC 1 cut(s) 3294
NlaIV GGNNCC 7 cut(s) 1183, 1950, 2289, 3034, 3552, 3940, 4076
NmeAIII GCCGAG 2 cut(s) 846, 4392
NmuCI GTSAC 6 cut(s) 638, 914, 947, 1161, 2068, 2332
NsiI ATGCAT 2 cut(s) 985, 2275
NspI RCATGY 7 cut(s) 636, 987, 2273, 2522, 2926, 3186, 3928
NspV TTCGAA 3 cut(s) 1789, 3233, 3288
OliI CACNNNNGTG 2 cut(s) 386, 522
PaeI GCATGC 3 cut(s) 987, 2273, 2522
PagI TCATGA 1 cut(s) 613
PciI ACATGT 2 cut(s) 2922, 3182
PciSI GCTCTTC 2 cut(s) 78, 462
PctI GAATGC 4 cut(s) 817, 2273, 2497, 4222
PdiI GCCGGC 1 cut(s) 3296
PdmI GAANNNNTTC 5 cut(s) 402, 1198, 1407, 3117, 4191
PfoI TCCNGGA 1 cut(s) 2226
PleI GAGTC 2 cut(s) 643, 2364
PpsI GAGTC 2 cut(s) 643, 2364
Ppu21I YACGTR 1 cut(s) 845
PscI ACATGT 2 cut(s) 2922, 3182
PshAI GACNNNNGTC 1 cut(s) 2895
PshBI ATTAAT 1 cut(s) 401
PsiI TTATAA 1 cut(s) 2408
Psp1406I AACGTT 1 cut(s) 2608
Psp6I CCWGG 6 cut(s) 2226, 2592, 3984, 3996, 4077, 4124
PspFI CCCAGC 4 cut(s) 815, 3277, 3298, 3659
PspGI CCWGG 6 cut(s) 2226, 2592, 3984, 3996, 4077, 4124
PspN4I GGNNCC 7 cut(s) 1183, 1950, 2289, 3034, 3552, 3940, 4076
PspPI GGNCC 4 cut(s) 1563, 2092, 2826, 3550
PsrI GAACNNNNNNTAC 2 cut(s) 2769, 2801
PstI CTGCAG 3 cut(s) 1127, 1219, 3133
PsuI RGATCY 1 cut(s) 2287
PvuII CAGCTG 3 cut(s) 1292, 1640, 3752
RseI CAYNNNNRTG 5 cut(s) 386, 522, 1070, 1725, 2994
SalI GTCGAC 1 cut(s) 4407
SapI GCTCTTC 2 cut(s) 78, 462
Sau96I GGNCC 4 cut(s) 1563, 2092, 2826, 3550
ScaI AGTACT 1 cut(s) 3346
SchI GAGTC 2 cut(s) 643, 2365
ScrFI CCNGG 8 cut(s) 2228, 2594, 3986, 3998, 4079, 4092, 4110, 4126
SduI GDGCHC 6 cut(s) 209, 2305, 3168, 3718, 3941, 4360
SfcI CTRYAG 5 cut(s) 520, 1123, 1215, 2574, 3129
SfuI TTCGAA 3 cut(s) 1789, 3233, 3288
SinI GGWCC 1 cut(s) 3550
SmiMI CAYNNNNRTG 5 cut(s) 386, 522, 1070, 1725, 2994
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
SpeI ACTAGT 2 cut(s) 274, 3347
SphI GCATGC 3 cut(s) 987, 2273, 2522
SspI AATATT 2 cut(s) 190, 4375
StyD4I CCNGG 8 cut(s) 2226, 2592, 3984, 3996, 4077, 4090, 4108, 4124
StyI CCWWGG 4 cut(s) 849, 1185, 1566, 3160
TaiI ACGT 6 cut(s) 847, 2611, 2895, 3695, 3933, 3975
TaqII GACCGA 1 cut(s) 2778
TatI WGTACW 9 cut(s) 655, 901, 1374, 2257, 3344, 3564, 3646, 4053, 4117
TauI GCSGC 1 cut(s) 3009
TscAI CASTG 9 cut(s) 355, 499, 1112, 1219, 1695, 3552, 3822, 4156, 4261
TseFI GTSAC 6 cut(s) 638, 914, 947, 1161, 2068, 2332
Tsp45I GTSAC 6 cut(s) 638, 914, 947, 1161, 2068, 2332
TspGWI ACGGA 2 cut(s) 2669, 3863
TspRI CASTG 9 cut(s) 355, 499, 1112, 1219, 1695, 3552, 3822, 4156, 4261
VneI GTGCAC 3 cut(s) 205, 2301, 3714
VpaK11BI GGWCC 1 cut(s) 3550
VspI ATTAAT 1 cut(s) 401
XapI RAATTY 8 cut(s) 98, 748, 1546, 1847, 3372, 3494, 3980, 4208
XceI RCATGY 7 cut(s) 636, 987, 2273, 2522, 2926, 3186, 3928
XcmI CCANNNNNNNNNTGG 1 cut(s) 1098
XmiI GTMKAC 2 cut(s) 1903, 4408
XmnI GAANNNNTTC 5 cut(s) 402, 1198, 1407, 3117, 4191
ZrmI AGTACT 1 cut(s) 3346
Zsp2I ATGCAT 2 cut(s) 985, 2275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.