Rw2G048900

Sister chromatid cohesion protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010273
Physical Location & Seq
Forward (+)
76404001 .. 76415970
11970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G048900.1

Sequence Viewer

Length: 4992 bp
ATGGGGCAAGTGCCTGGCAGTAGTACAGTACCAGATAAGAAGCCCTTTGTGCCAATTATACCTATAACAAGAACTTCTGAAAGAGATTATGGTGCAACTCACAATAATCAGCTCAACTATACTCCCCCAAATGACATATCTATAGCATCCTCCAGAAAACCAAAAGCAAAGAGAAAAGCTAGTGATGGCGTTACTTCCTCAGTTGGGTCAGATCCCGTTGCAAGTCAAGAAGCCACTGTTGGATGCTTCTGTGAGTTGGTGGAGGATTTTTGCGGCAGAGCTGAAATTTTCAGTGAGGATCGGGACGAAGCACAGTGGTTATCTATGCCTCTTTCTGATCTTAGAATGCTTGCAAATGAAATAATGTCTCTTCGCGCAAAGAGGCTTCTACATATGGTTCCTGTAGATACTTTTGTTAGATTGTTAAGGATTTTAGATCATCAGATACATGGAGCTGAAGGCTTGTCTATAAATGAACAGTCAGATTCAAATGGGGTTTCATCAATCAATTGTGCACTAGAGTCCATTCATGCAGCTTTAGCAGTAATGGCCCATAACCAGATGCCAAAGCAGCTGTATAAAGAAGAGATCATTGAAAGAATTCTGGAGTTCTCCAGGCATCAGATAATGGATGTTATGTGTGCTTGCGATCCATCATATCGTGCTTTACATAGGCCAAGCGAAAATGGGACACTTGAAGTTGAGGAAGACGAAATTCTTGATGCTGAAACTGGTTCAGCAAGCAAGAGGCGGCGTAGTACTAAGACTGTTAAAGTGCAGAAATCATCATATAACAGGGTCTCTGCTGCTGTGAATAATATTCTTCAGAAAATGTGCACAATTCTTGGTTTGCTAAAGGATTTGTTGCTGATAGAAAAGTTGTCAGATAGTTGTATCTTACAACTACTGAAAACAAGCTTTACTACATTTATGGTGGATAATATCCAGCTTTTGCAACTGAAGGCAATTGGTTTGATCAGTGGGATATACTATTCATACACTCAACATCGGACTTATGTTATAGATGAGTTACTTCAGCTGCTTTGGAAGTTGCCTTTCTCGAAGCGAGCTTTGAGATCCTATCACCTACCTGATGAAGAGCAAACGCAGATTCAAATGATCACTGCTTTGCTGATTCAGTTGGTTCACTGTAGTGCAAACCTTCCTGCACCTTTAAGGCAGGATTCAAGTGTTAATTCCATCTTAGAAGTTTCTGACAATGCTGATTATCCAATTAAAGGCCTTGAGGCAGCACAAGCAGCATGTTGTCACTTTTGGAAAGGGGTACTTCAGCGGTTTGCTAATGTCAAGAACCAGGAAGCTTCAGAATTCAAAGTTATGATGGAGAATCTTGTTACTGATCTGCTGACAACATTGAATTTACCTGAATATCCCGCTTCAGCCCCTATTCTGGAGGTTCTTTGTGTCTTGTTACTCGCGAATGCTGGGGTGAAATCTAAAGATGTAGCTGCACGTAGCATGGCTATCGATCTTCTAGGAACCATTGCTGCGAGGTTGAAGCGTGATTCTCTCCTTCGCAGTAGGAGCAAATTTTGGATATTACAGGAGTTGGTTAGTGGGGATGCGGCTGATCAGACTTATCCAAAAGATGTATGTTCTGCTTGTTTAGATGGCAGGGTTGAAAAGACTTTCTTTGTATGTCAAGGTTGTCAGAGAATGTTTCACGCAGATTGCATGGGAGTAAGAGAGCATGAAGTTCCTAACCAATCTTGGCACTGTCAGATATGTCTCTGCCGGAAACAACTTCTTGTTTTACAATCTTACTGCAAGTCACAATACAAAGATGATGGATTTAAGGATCATAAAAGTTCAGGAAGAAAAACTGAAGTTACTTTTTCAATTACTAAACTTGAGATCGTCCAACAGTTGCTTTTGAATTACCTACAAGATACCGCATCAGCTGATGATGTTCATCTCTTTATTCGTTGGCTTTATGTCTGCTTGTGGTACAAGGATGATCCACCAAAGTCCCAGCAGAAGTTCATTTACTATCTTGCAAGACTGAACTCAAAAGCAATAGTGCGGGATTCTGGGACTGTTTTTTCCTTGCTGACAAGGGACTTAATCAAGCAGATTACCTTAGTATTGGGACGAAATACTTCTTTTGCCAGAGGATTTGATAAGATTCTACACCTGCTTCTGGCAAGTCTAAGGGAGAATTCTCCTGTAATCAGGGCCAAGGCTTTACGAGCGGTTAGTATAGTTGTAGAGGCTGATCCGGAGGTTTTAGGAGACAAACGTGTGCAACCAGCTGTTGAAGGAAGGTTTTGTGATTCCGCAATTTCTGTTAGAGAAGCAGCACTGGAACTCGTAGGGAGGCATATTGCTTCCCATCCTGATGTTGGTTTGAAGTATTTTGAGAAGGTTGCTGAGAGAATCAAAGATACAGGTGTGAGTGTTCGAAAGCGATCTATTAAAATCATACGAGATATGTGTGTTTCAAATGGGGACTTCTCTGAATTTACTAGTGCTTGCATTGCTATCATCTCCCGTATTGGTGACGACGAATCAAGTATACAGGATCTTGTTTGCAAGACATTTTATGAGTTCTGGTTTGAGGAACATACTGGTTCACATACTCAATTCTTTGGAGATGATAGCTCTGTTCCATTGGAGGTGGCTAAAAAAACTGAACAGATTGTTGAGATGTTGAGGAGGATGCCAACACCACACCATCTTGTTACCGTCATTAAACGCAACTTAGCCCTTGATTTTTTCCCACAAGCAGCTAAAGCTGCGGGAATCAATCCCGTCTTACTTGCATCAGTACGCAATCGGTGTGAGCTAATGTGCAAGTTTTTACTGGAAAGGATATTGCAGGTAGAAGAAATGAATATCCAGGAAGTTGAGATGTGTGCACTTCCATATGTGCAAGTTCTGCATGCATTTTGTGTTGTGGATCCAACATTATGTGCACCGGTTTCTAACCCTTCGCAGTTTGTGGTCACTCTTCAGCCTTATCTAAAGAGTCAGGATGATAATCGAGTGGTTGCCAAGTTATTGGAGAGCATAATATTCATAATTGATTCTGTCCTGCCTTTAGTAAGAAAGTTGCCACAAAATGTTCTTGAAGAACTTGAACAAGACTTAAAGAGCATGATTGTTCGGCATTCCTTCTTGTCAGTTGTTCATGCTTGCATCAAGTGCCTCTGTGCTGTGAGTAAAGTGGCAGGAAAAGGCGCTGCTGTTGTGGAGTATCTAATTCAGGTTTTTTACAAACGGCTGGATGCTGAGGAAGTTGACAACCAGCAGAAAGCGGGGCGATCTCTTTTCTGTCTTGGAATGCTTATCCGTTATGGCAACTCTTTACTGTGTTACTCTGATCAAACAATTGATGTTGCAAGCAGTCTCGGCTTATTTAAAAGATATCTTCTAATGGACGATTTTTTCTTAAAGGCTAGATCATTGCAGGCATTGGGCTTTGTGTTAATTGCTAGGCCTGAATTTATGTTGGAAAAAGATATTGGGAAGATATTAGAGGAGACATTCTCTTCTAGCTCTGATGTTCGTCTCAAGATGCAAGCTTTGCAAAACATGTACGACTATCTTCTTGATGCTGAAACTCAATTGGGAACGGATACAACCGGTAATAATGTGGCTGATTATTCTGTGGAGGGTGGCAATGCTGTACCTGTTGCTGCGGGTGCTGGAGATACTAATATATGTGGCGGAATAGTTCAGTTGTATTGGGATAATATTCTGGAGAGATGTTTGGACTTTAATGAACAAATTCGCAGCTCTGCCCTTAAGATTGTTGAAGTTGTGCTGCGCCAAGGGCTTGTGCACCCTATTACTTGTGTTCCATTCCTCATTGCACTGGAAACAGATCCACTGGAGGCCAATTCGACATTAGCGCATCATTTGCTGATGAATATGAATGAAAAGTACCCTTCATTTTTTGAAAGCCGACTAGGGGATGGCCTTCAAATGTCTTTTGGATTTATACAATCCATACGTCCAAGTACTGAACGTGAGAACACAAAACCACCATCAAAGGCTTCTGGAAATGCAAAGGGAAAAGTTGATGATGTTTCTTTTGCTCAAGCAAGATTGGGAGTCTCTCGAATCTACAAGCTTATTCGTGCTAACCGTGTTTCAAGGAACAAATTTATGTCCTCAATTGTTCGCAAGTTTGATAATCCTATCTGGACCACTTCAGTAGTCCCTTTCCTCATGTACTGCACGGAAATACTTGCCTTGCTGCCATTCACAACACCTGATGAGCCCCTTTATTTAGTCTATGCTATAAATAGAGTAATACAAGTTAAGGCTGGGCAACTTGAAGCAAAGCTGAAAGCTCTGACATTGCATCTGTTACAAAGAGGTGTACCCCGTGGGAATGGCGTAATTAAAGAGGACCCAGCTGCTCCGCCCTTTACAAGAGGAATGGCATCAGTGGATTTGAATGGGACAATTGAACCAGAATCAGCTTATTACATGGCAGCTATGGATTTGAATGGGACGATTGAACAAGACCCAGCTGATCAGTCTGTCTCAAATCATGATACCGTGTTAGAGGCAAAGATGCACGGTAAAGGTTCTGTCAGTTCCTCTGGCATCTCAATAGATGATGTACAGATAATCCAGGCAGACTGCCTGGCAGCTATTGCATTGCAGCTTCTTTTGAAACTGAAGAGACACCTAAAAATTGTGTATAGCTTGAATGATGCTAGGTGCCAGGCTTTCTCTCCAACCGATCCCATTAAACCTGGAGATGCTTTCTCCAAGCAGAGCATTCCTTTTGACGTCAGTGACACACATACTAACTTGCCTGGAACTTACCAAGAATTGGTACAAAGATATCAGGAATTCAAGAATGCGTTGAAGGATGATACAGTTGATTTCTCAACTTACACAGCAAATCTCAAAAGAAAACGTCCGGCCCCTAGGAAAGGAAGGAAATCTGGAGCAAGAGATGATGAGGATGACAATGATGATGATGAAGATTGGTCAGGTGGGCTCGGAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000070 GO:0000122 GO:0000278 GO:0000280 GO:0000428 GO:0000819 GO:0001654 GO:0001655 GO:0001656 GO:0001822 GO:0003006 GO:0003007 GO:0003008 GO:0003151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007064 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0007423 GO:0007507 GO:0007548 GO:0007600 GO:0007605 GO:0008022 GO:0008104 GO:0008150 GO:0009314 GO:0009628 GO:0009653 GO:0009790 GO:0009887 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010165 GO:0010171 GO:0010212 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010720 GO:0016043 GO:0016591 GO:0019219 GO:0019222 GO:0019899 GO:0019904 GO:0022008 GO:0022402 GO:0022414 GO:0030154 GO:0030326 GO:0030334 GO:0030335 GO:0030880 GO:0031056 GO:0031058 GO:0031063 GO:0031065 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032039 GO:0032116 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0033365 GO:0033554 GO:0034085 GO:0034086 GO:0034088 GO:0034502 GO:0034613 GO:0035107 GO:0035108 GO:0035112 GO:0035113 GO:0035115 GO:0035136 GO:0035239 GO:0035261 GO:0035295 GO:0040007 GO:0040008 GO:0040012 GO:0040017 GO:0042471 GO:0042634 GO:0042826 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043388 GO:0043583 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045597 GO:0045892 GO:0045934 GO:0045995 GO:0047485 GO:0048285 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048546 GO:0048557 GO:0048562 GO:0048565 GO:0048566 GO:0048568 GO:0048589 GO:0048592 GO:0048598 GO:0048608 GO:0048638 GO:0048699 GO:0048731 GO:0048736 GO:0048806 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0050896 GO:0050954 GO:0051094 GO:0051098 GO:0051099 GO:0051101 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051253 GO:0051270 GO:0051272 GO:0051276 GO:0051641 GO:0051716 GO:0051960 GO:0051962 GO:0055029 GO:0055123 GO:0060065 GO:0060173 GO:0060255 GO:0060284 GO:0060322 GO:0060323 GO:0060324 GO:0060325 GO:0061008 GO:0061010 GO:0061038 GO:0061458 GO:0061695 GO:0065007 GO:0065009 GO:0070013 GO:0070087 GO:0070727 GO:0071168 GO:0071214 GO:0071478 GO:0071479 GO:0071481 GO:0071840 GO:0071921 GO:0072001 GO:0072359 GO:0080090 GO:0090311 GO:0090312 GO:0090596 GO:0090694 GO:0098813 GO:0104004 GO:0140014 GO:1902275 GO:1902494 GO:1902679 GO:1903047 GO:1903506 GO:1903507 GO:1905269 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2000145 GO:2000147 GO:2001141 GO:2001222 GO:2001224 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04111 map04111
Pfam Domains
Protein Families
Cohesin_HEAT Nipped-B_C PHD

Protein Analysis

1663

Amino Acids

185.82

Weight (kDa)

6.26

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cohesin_HEAT PF12765 737 - 778 1.1e-12 HEAT repeat associated with sister chromatid cohesion
Nipped-B_C PF12830 1229 - 1426 2.1e-51 Sister chromatid cohesion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15540 AT5G15540
fragaria_vesca FvH4_6g47080 FvH4_6g47080 FvH4_6g47080
malus_domestica MD09G1064300.v1.1 MD17G1057600.v1.1
prunus_persica Prupe.3G256700_v2.0.a1
pyrus_communis pycom111g05400 pycom17g05770
rosa_chinensis RchiOBHm_Chr2g0166131
rosa_laevigata RLG00000021588
rosa_multiflora Rmu_sc0006218.1_g000028
rosa_roxburghii Rroxscaffold_2G00085180
rosa_rugosa Rorug02G0520500 Rorug02G0520600
rosa_samantha Rh2AG586600 Rh2BG598400 Rh2CG569100 Rh2DG608300
rosa_wichuraiana Rw2G048900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2163
AatII GACGTC 1 cut(s) 4770
Acc36I ACCTGC 2 cut(s) 2163, 2830
AccB1I GGYRCC 1 cut(s) 4695
AccB7I CCANNNNNTGG 1 cut(s) 4810
AccBSI CCGCTC 1 cut(s) 2213
AccI GTMKAC 1 cut(s) 2536
AccII CGCG 2 cut(s) 375, 1439
AccIII TCCGGA 1 cut(s) 2239
AcyI GRCGYC 1 cut(s) 4767
AfiI CCNNNNNNNGG 8 cut(s) 204, 1238, 1411, 2161, 2363, 3934, 4810, 4913
AflII CTTAAG 1 cut(s) 3767
AflIII ACRYGT 2 cut(s) 2260, 3555
AgeI ACCGGT 2 cut(s) 2938, 3605
AhlI ACTAGT 1 cut(s) 2486
AjnI CCWGG 9 cut(s) 13, 614, 1314, 2859, 4605, 4617, 4698, 4729, 4792
AjuI GAANNNNNNNTTGG 4 cut(s) 222, 254, 3468, 3500
AleI CACNNNNGTG 1 cut(s) 1152
Alw21I GWGCWC 5 cut(s) 517, 839, 2881, 2938, 3807
Alw44I GTGCAC 5 cut(s) 513, 835, 2877, 2934, 3803
Aor13HI TCCGGA 1 cut(s) 2239
AoxI GGCC 8 cut(s) 549, 674, 1240, 2196, 3458, 3858, 3940, 4902
ApaLI GTGCAC 5 cut(s) 513, 835, 2877, 2934, 3803
AsiGI ACCGGT 2 cut(s) 2938, 3605
Asp700I GAANNNNTTC 3 cut(s) 600, 2119, 3750
AspA2I CCTAGG 1 cut(s) 4907
AspLEI GCGC 4 cut(s) 377, 3203, 3792, 3877
AspS9I GGNCC 5 cut(s) 550, 2196, 4170, 4378, 4903
AsuHPI GGTGA 3 cut(s) 1076, 1462, 2531
AsuII TTCGAA 1 cut(s) 2422
AvaII GGWCC 2 cut(s) 4170, 4378
AvrII CCTAGG 1 cut(s) 4907
BaeGI GKGCMC 5 cut(s) 517, 839, 2881, 2938, 3807
BaeI ACNNNNGTAYC 4 cut(s) 399, 432, 2397, 2430
BamHI GGATCC 1 cut(s) 2920
BanI GGYRCC 1 cut(s) 4695
BanII GRGCYC 2 cut(s) 4248, 4983
BarI GAAGNNNNNNTAC 2 cut(s) 1991, 2023
BbsI GAAGAC 1 cut(s) 714
Bbv12I GWGCWC 5 cut(s) 517, 839, 2881, 2938, 3807
BbvCI CCTCAGC 1 cut(s) 3252
BceAI ACGGC 1 cut(s) 3257
BcgI CGANNNNNNTGC 2 cut(s) 1468, 1502
BciT130I CCWGG 9 cut(s) 15, 616, 1316, 2861, 4607, 4619, 4700, 4731, 4794
BciVI GTATCC 1 cut(s) 3592
BclI TGATCA 5 cut(s) 975, 1119, 1591, 3343, 4504
BcuI ACTAGT 1 cut(s) 2486
BfmI CTRYAG 3 cut(s) 141, 402, 1150
BfoI RGCGCY 1 cut(s) 3204
BfrI CTTAAG 1 cut(s) 3767
BfuAI ACCTGC 2 cut(s) 2163, 2830
BfuI GTATCC 1 cut(s) 3592
BlnI CCTAGG 1 cut(s) 4907
BmcAI AGTACT 2 cut(s) 760, 3985
Bme1390I CCNGG 9 cut(s) 15, 616, 1316, 2861, 4607, 4619, 4700, 4731, 4794
Bme18I GGWCC 2 cut(s) 4170, 4378
BmgT120I GGNCC 5 cut(s) 550, 2196, 4170, 4378, 4903
BmiI GGNNCC 6 cut(s) 399, 1501, 2922, 4380, 4697, 4905
BmrFI CCNGG 9 cut(s) 15, 616, 1316, 2861, 4607, 4619, 4700, 4731, 4794
BpiI GAAGAC 1 cut(s) 714
BplI GAGNNNNNCTC 2 cut(s) 3494, 3526
BpmI CTGGAG 9 cut(s) 136, 598, 626, 1433, 3690, 3743, 3875, 4752, 4947
Bpu10I CCTNAGC 1 cut(s) 3252
Bpu14I TTCGAA 1 cut(s) 2422
BpuEI CTTGAG 4 cut(s) 1265, 1892, 3518, 4047
Bsa29I ATCGAT 1 cut(s) 1488
BsaAI YACGTR 1 cut(s) 1475
BsaBI GATNNNNATC 2 cut(s) 1932, 3000
BsaHI GRCGYC 1 cut(s) 4767
BsaI GGTCTC 1 cut(s) 805
BsaJI CCNNGG 4 cut(s) 2199, 3793, 4354, 4907
BsaWI WCCGGW 3 cut(s) 2239, 2938, 3605
Bsc4I CCNNNNNNNGG 8 cut(s) 204, 1238, 1411, 2161, 2363, 3934, 4810, 4913
Bse118I RCCGGY 2 cut(s) 2938, 3605
Bse1I ACTGG 6 cut(s) 736, 2328, 2593, 2829, 3843, 3858
Bse3DI GCAATG 7 cut(s) 1503, 2496, 3425, 3649, 3831, 4325, 4631
Bse8I GATNNNNATC 2 cut(s) 1932, 3000
BseAI TCCGGA 1 cut(s) 2239
BseBI CCWGG 9 cut(s) 15, 616, 1316, 2861, 4607, 4619, 4700, 4731, 4794
BseCI ATCGAT 1 cut(s) 1488
BseDI CCNNGG 4 cut(s) 2199, 3793, 4354, 4907
BseJI GATNNNNATC 2 cut(s) 1932, 3000
BseLI CCNNNNNNNGG 8 cut(s) 204, 1238, 1411, 2161, 2363, 3934, 4810, 4913
BseMI GCAATG 7 cut(s) 1503, 2496, 3425, 3649, 3831, 4325, 4631
BseMII CTCAG 3 cut(s) 213, 2382, 3243
BseNI ACTGG 6 cut(s) 736, 2328, 2593, 2829, 3843, 3858
BseRI GAGGAG 2 cut(s) 2689, 3515
BseSI GKGCMC 5 cut(s) 517, 839, 2881, 2938, 3807
BseYI CCCAGC 5 cut(s) 1445, 1992, 4292, 4381, 4498
BsgI GTGCAG 4 cut(s) 797, 1152, 1455, 4186
Bsh1236I CGCG 2 cut(s) 375, 1439
BshFI GGCC 8 cut(s) 551, 676, 1242, 2198, 3460, 3860, 3942, 4904
BshNI GGYRCC 1 cut(s) 4695
BshTI ACCGGT 2 cut(s) 2938, 3605
BshVI ATCGAT 1 cut(s) 1488
BsiHKAI GWGCWC 5 cut(s) 517, 839, 2881, 2938, 3807
BsiSI CCGG 5 cut(s) 1756, 2240, 2939, 3606, 4901
BslI CCNNNNNNNGG 8 cut(s) 204, 1238, 1411, 2161, 2363, 3934, 4810, 4913
BsmBI CGTCTC 1 cut(s) 3536
BsmI GAATGC 6 cut(s) 351, 1447, 3130, 3309, 4755, 4843
BsnI GGCC 8 cut(s) 551, 676, 1242, 2198, 3460, 3860, 3942, 4904
Bso31I GGTCTC 1 cut(s) 805
Bsp119I TTCGAA 1 cut(s) 2422
Bsp1286I GDGCHC 7 cut(s) 517, 839, 2881, 2938, 3807, 4248, 4983
Bsp13I TCCGGA 1 cut(s) 2239
Bsp1407I TGTACA 1 cut(s) 4594
Bsp68I TCGCGA 1 cut(s) 1439
BspANI GGCC 8 cut(s) 551, 676, 1242, 2198, 3460, 3860, 3942, 4904
BspCNI CTCAG 3 cut(s) 212, 2383, 3244
BspDI ATCGAT 1 cut(s) 1488
BspEI TCCGGA 1 cut(s) 2239
BspFNI CGCG 2 cut(s) 375, 1439
BspHI TCATGA 1 cut(s) 4522
BspLI GGNNCC 6 cut(s) 399, 1501, 2922, 4380, 4697, 4905
BspMI ACCTGC 2 cut(s) 2163, 2830
BspQI GCTCTTC 1 cut(s) 1092
BspT104I TTCGAA 1 cut(s) 2422
BspT107I GGYRCC 1 cut(s) 4695
BspTI CTTAAG 1 cut(s) 3767
BspTNI GGTCTC 1 cut(s) 805
BsrBI CCGCTC 1 cut(s) 2213
BsrDI GCAATG 7 cut(s) 1503, 2496, 3425, 3649, 3831, 4325, 4631
BsrFI RCCGGY 2 cut(s) 2938, 3605
BsrGI TGTACA 1 cut(s) 4594
BsrI ACTGG 6 cut(s) 736, 2328, 2593, 2829, 3843, 3858
BssAI RCCGGY 2 cut(s) 2938, 3605
BssECI CCNNGG 4 cut(s) 2199, 3793, 4354, 4907
BssNAI GTATAC 1 cut(s) 2537
BssNI GRCGYC 1 cut(s) 4767
BssT1I CCWWGG 3 cut(s) 2199, 3793, 4907
Bst1107I GTATAC 1 cut(s) 2537
Bst2UI CCWGG 9 cut(s) 15, 616, 1316, 2861, 4607, 4619, 4700, 4731, 4794
Bst6I CTCTTC 6 cut(s) 375, 579, 1092, 2976, 3517, 4649
BstACI GRCGYC 1 cut(s) 4767
BstAFI CTTAAG 1 cut(s) 3767
BstAPI GCANNNNNTGC 5 cut(s) 2899, 3165, 3547, 3883, 4628
BstAUI TGTACA 1 cut(s) 4594
BstBAI YACGTR 1 cut(s) 1475
BstBI TTCGAA 1 cut(s) 2422
BstDEI CTNAG 9 cut(s) 199, 341, 762, 1204, 2101, 2171, 2391, 2722, 3252
BstDSI CCRYGG 1 cut(s) 4354
BstENI CCTNNNNNAGG 1 cut(s) 4911
BstFNI CGCG 2 cut(s) 375, 1439
BstH2I RGCGCY 1 cut(s) 3204
BstHHI GCGC 4 cut(s) 377, 3203, 3792, 3877
BstNI CCWGG 9 cut(s) 15, 616, 1316, 2861, 4607, 4619, 4700, 4731, 4794
BstNSI RCATGY 3 cut(s) 1266, 2906, 3559
BstSCI CCNGG 9 cut(s) 13, 614, 1314, 2859, 4605, 4617, 4698, 4729, 4792
BstSFI CTRYAG 3 cut(s) 141, 402, 1150
BstSLI GKGCMC 5 cut(s) 517, 839, 2881, 2938, 3807
BstUI CGCG 2 cut(s) 375, 1439
BstV2I GAAGAC 1 cut(s) 714
BstX2I RGATCY 5 cut(s) 211, 1076, 2542, 2920, 3847
BstXI CCANNNNNNTGG 1 cut(s) 3022
BstYI RGATCY 5 cut(s) 211, 1076, 2542, 2920, 3847
BstZ17I GTATAC 1 cut(s) 2537
Bsu15I ATCGAT 1 cut(s) 1488
BsuI GTATCC 1 cut(s) 3592
BsuRI GGCC 8 cut(s) 551, 676, 1242, 2198, 3460, 3860, 3942, 4904
BsuTUI ATCGAT 1 cut(s) 1488
BtgI CCRYGG 1 cut(s) 4354
BtsI GCAGTG 1 cut(s) 1122
BtuMI TCGCGA 1 cut(s) 1439
BveI ACCTGC 2 cut(s) 2163, 2830
CciI TCATGA 1 cut(s) 4522
CfoI GCGC 4 cut(s) 377, 3203, 3792, 3877
Cfr10I RCCGGY 2 cut(s) 2938, 3605
Cfr13I GGNCC 5 cut(s) 550, 2196, 4170, 4378, 4903
ClaI ATCGAT 1 cut(s) 1488
CspAI ACCGGT 2 cut(s) 2938, 3605
DdeI CTNAG 9 cut(s) 199, 341, 762, 1204, 2101, 2171, 2391, 2722, 3252
DraI TTTAAA 1 cut(s) 3382
Eam1104I CTCTTC 6 cut(s) 375, 579, 1092, 2976, 3517, 4649
EarI CTCTTC 6 cut(s) 375, 579, 1092, 2976, 3517, 4649
EciI GGCGGA 2 cut(s) 3705, 4380
Eco130I CCWWGG 3 cut(s) 2199, 3793, 4907
Eco147I AGGCCT 2 cut(s) 1242, 3460
Eco24I GRGCYC 2 cut(s) 4248, 4983
Eco31I GGTCTC 1 cut(s) 805
Eco32I GATATC 2 cut(s) 3389, 4823
Eco47I GGWCC 2 cut(s) 4170, 4378
EcoNI CCTNNNNNAGG 1 cut(s) 4911
EcoO109I RGGNCCY 1 cut(s) 4378
EcoRI GAATTC 4 cut(s) 600, 1328, 2179, 4829
EcoRII CCWGG 9 cut(s) 13, 614, 1314, 2859, 4605, 4617, 4698, 4729, 4792
EcoRV GATATC 2 cut(s) 3389, 4823
EcoT14I CCWWGG 3 cut(s) 2199, 3793, 4907
EcoT22I ATGCAT 1 cut(s) 2908
EcoT38I GRGCYC 2 cut(s) 4248, 4983
ErhI CCWWGG 3 cut(s) 2199, 3793, 4907
Esp3I CGTCTC 1 cut(s) 3536
FalI AAGNNNNNCTT 6 cut(s) 29, 61, 1272, 1304, 1637, 1669
FauI CCCGC 5 cut(s) 1402, 2037, 2752, 3271, 3655
FauNDI CATATG 2 cut(s) 393, 2887
FbaI TGATCA 5 cut(s) 975, 1119, 1591, 3343, 4504
FblI GTMKAC 1 cut(s) 2536
FriOI GRGCYC 2 cut(s) 4248, 4983
GlaI GCGC 4 cut(s) 376, 3202, 3791, 3876
GsaI CCCAGC 5 cut(s) 1449, 1996, 4296, 4385, 4502
GsuI CTGGAG 9 cut(s) 136, 598, 626, 1433, 3690, 3743, 3875, 4752, 4947
HaeII RGCGCY 1 cut(s) 3204
HaeIII GGCC 8 cut(s) 551, 676, 1242, 2198, 3460, 3860, 3942, 4904
HapII CCGG 5 cut(s) 1756, 2240, 2939, 3606, 4901
HhaI GCGC 4 cut(s) 377, 3203, 3792, 3877
Hin1I GRCGYC 1 cut(s) 4767
Hin6I GCGC 4 cut(s) 375, 3201, 3790, 3875
HinP1I GCGC 4 cut(s) 375, 3201, 3790, 3875
HincII GTYRAC 1 cut(s) 3262
HindII GTYRAC 1 cut(s) 3262
HindIII AAGCTT 4 cut(s) 916, 1320, 3543, 4094
HpaII CCGG 5 cut(s) 1756, 2240, 2939, 3606, 4901
HphI GGTGA 3 cut(s) 1076, 1462, 2531
Hpy99I CGWCG 1 cut(s) 2528
HpyCH4IV ACGT 6 cut(s) 1474, 2260, 3976, 3991, 4767, 4897
HpyF3I CTNAG 9 cut(s) 199, 341, 762, 1204, 2101, 2171, 2391, 2722, 3252
HpySE526I ACGT 6 cut(s) 1474, 2260, 3976, 3991, 4767, 4897
Hsp92I GRCGYC 1 cut(s) 4767
HspAI GCGC 4 cut(s) 375, 3201, 3790, 3875
Kpn2I TCCGGA 1 cut(s) 2239
Ksp22I TGATCA 5 cut(s) 975, 1119, 1591, 3343, 4504
LguI GCTCTTC 1 cut(s) 1092
LmnI GCTCC 4 cut(s) 452, 1545, 4393, 4928
MaeII ACGT 6 cut(s) 1474, 2260, 3976, 3991, 4767, 4897
MbiI CCGCTC 1 cut(s) 2213
MfeI CAATTG 6 cut(s) 508, 966, 3351, 3587, 4140, 4434
MflI RGATCY 5 cut(s) 211, 1076, 2542, 2920, 3847
MhlI GDGCHC 7 cut(s) 517, 839, 2881, 2938, 3807, 4248, 4983
MlyI GAGTC 3 cut(s) 530, 2998, 4086
MmeI TCCRAC 5 cut(s) 220, 1906, 2948, 3453, 4736
Mph1103I ATGCAT 1 cut(s) 2908
MroI TCCGGA 1 cut(s) 2239
MroXI GAANNNNTTC 3 cut(s) 600, 2119, 3750
MslI CAYNNNNRTG 2 cut(s) 1152, 2358
MspA1I CMGCKG 7 cut(s) 574, 1039, 1294, 1922, 2273, 4385, 4502
MspCI CTTAAG 1 cut(s) 3767
MspI CCGG 5 cut(s) 1756, 2240, 2939, 3606, 4901
MspR9I CCNGG 9 cut(s) 15, 616, 1316, 2861, 4607, 4619, 4700, 4731, 4794
MunI CAATTG 6 cut(s) 508, 966, 3351, 3587, 4140, 4434
Mva1269I GAATGC 6 cut(s) 351, 1447, 3130, 3309, 4755, 4843
MvaI CCWGG 9 cut(s) 15, 616, 1316, 2861, 4607, 4619, 4700, 4731, 4794
MvnI CGCG 2 cut(s) 375, 1439
NdeI CATATG 2 cut(s) 393, 2887
NlaIV GGNNCC 6 cut(s) 399, 1501, 2922, 4380, 4697, 4905
NmeAIII GCCGAG 1 cut(s) 3351
NmuCI GTSAC 5 cut(s) 1268, 1791, 2519, 2965, 4772
NruI TCGCGA 1 cut(s) 1439
NsiI ATGCAT 1 cut(s) 2908
NspI RCATGY 3 cut(s) 1266, 2906, 3559
NspV TTCGAA 1 cut(s) 2422
OliI CACNNNNGTG 1 cut(s) 1152
PaeI GCATGC 1 cut(s) 2906
PagI TCATGA 1 cut(s) 4522
PaqCI CACCTGC 1 cut(s) 2163
PceI AGGCCT 2 cut(s) 1242, 3460
PciI ACATGT 1 cut(s) 3555
PciSI GCTCTTC 1 cut(s) 1092
PcsI WCGNNNNNNNCGW 1 cut(s) 4108
PctI GAATGC 6 cut(s) 351, 1447, 3130, 3309, 4755, 4843
PdmI GAANNNNTTC 3 cut(s) 600, 2119, 3750
PflMI CCANNNNNTGG 1 cut(s) 4810
PfoI TCCNGGA 1 cut(s) 2859
PinAI ACCGGT 2 cut(s) 2938, 3605
PleI GAGTC 3 cut(s) 529, 2997, 4085
PpsI GAGTC 3 cut(s) 529, 2997, 4085
Ppu21I YACGTR 1 cut(s) 1475
PpuMI RGGWCCY 1 cut(s) 4378
PscI ACATGT 1 cut(s) 3555
Psp5II RGGWCCY 1 cut(s) 4378
Psp6I CCWGG 9 cut(s) 13, 614, 1314, 2859, 4605, 4617, 4698, 4729, 4792
PspFI CCCAGC 5 cut(s) 1445, 1992, 4292, 4381, 4498
PspGI CCWGG 9 cut(s) 13, 614, 1314, 2859, 4605, 4617, 4698, 4729, 4792
PspN4I GGNNCC 6 cut(s) 399, 1501, 2922, 4380, 4697, 4905
PspPI GGNCC 5 cut(s) 550, 2196, 4170, 4378, 4903
PspPPI RGGWCCY 1 cut(s) 4378
PsuI RGATCY 5 cut(s) 211, 1076, 2542, 2920, 3847
PvuII CAGCTG 6 cut(s) 574, 1039, 1922, 2273, 4385, 4502
RruI TCGCGA 1 cut(s) 1439
RseI CAYNNNNRTG 2 cut(s) 1152, 2358
SapI GCTCTTC 1 cut(s) 1092
Sau96I GGNCC 5 cut(s) 550, 2196, 4170, 4378, 4903
ScaI AGTACT 2 cut(s) 760, 3985
SchI GAGTC 3 cut(s) 530, 2998, 4086
ScrFI CCNGG 9 cut(s) 15, 616, 1316, 2861, 4607, 4619, 4700, 4731, 4794
SduI GDGCHC 7 cut(s) 517, 839, 2881, 2938, 3807, 4248, 4983
SfcI CTRYAG 3 cut(s) 141, 402, 1150
SfuI TTCGAA 1 cut(s) 2422
SinI GGWCC 2 cut(s) 4170, 4378
SmiMI CAYNNNNRTG 2 cut(s) 1152, 2358
SmlI CTYRAG 5 cut(s) 1244, 1871, 3533, 3767, 4062
SmoI CTYRAG 5 cut(s) 1244, 1871, 3533, 3767, 4062
SpeI ACTAGT 1 cut(s) 2486
SphI GCATGC 1 cut(s) 2906
SseBI AGGCCT 2 cut(s) 1242, 3460
SspI AATATT 3 cut(s) 820, 3036, 3718
StuI AGGCCT 2 cut(s) 1242, 3460
StyD4I CCNGG 9 cut(s) 13, 614, 1314, 2859, 4605, 4617, 4698, 4729, 4792
StyI CCWWGG 3 cut(s) 2199, 3793, 4907
TaiI ACGT 6 cut(s) 1477, 2263, 3979, 3994, 4770, 4900
TaqI TCGA 6 cut(s) 1061, 1488, 2422, 3004, 3866, 4084
TatI WGTACW 5 cut(s) 23, 758, 3983, 4197, 4594
TauI GCSGC 3 cut(s) 276, 754, 1589
TseFI GTSAC 5 cut(s) 1268, 1791, 2519, 2965, 4772
Tsp45I GTSAC 5 cut(s) 1268, 1791, 2519, 2965, 4772
TspGWI ACGGA 3 cut(s) 3302, 3611, 4220
Van91I CCANNNNNTGG 1 cut(s) 4810
Vha464I CTTAAG 1 cut(s) 3767
VneI GTGCAC 5 cut(s) 513, 835, 2877, 2934, 3803
VpaK11BI GGWCC 2 cut(s) 4170, 4378
XagI CCTNNNNNAGG 1 cut(s) 4911
XceI RCATGY 3 cut(s) 1266, 2906, 3559
XcmI CCANNNNNNNNNTGG 1 cut(s) 2360
XmaJI CCTAGG 1 cut(s) 4907
XmiI GTMKAC 1 cut(s) 2536
XmnI GAANNNNTTC 3 cut(s) 600, 2119, 3750
ZraI GACGTC 1 cut(s) 4768
ZrmI AGTACT 2 cut(s) 760, 3985
Zsp2I ATGCAT 1 cut(s) 2908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.