MD17G1057600.v1.1

Sister chromatid cohesion protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010273
Physical Location & Seq
Reverse (-)
4695370 .. 4707434
12065 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1057600.v1.1.491

Sequence Viewer

Length: 5424 bp
ATGAGTTATCCGGTAGTCACTTCGAGCTCGAGCTCCGGCTCGGCTCACGGCGGCATCAGCCTCTCCAACACGGTGCACTCGGAGGTCGCGCCTTGCTTGCCTCTGCCATCGCTGCCGGTGTTCTGCGGCGCATCTGATCACCTCCGGCTGTTCGACGAGCCGAGTCGGAACAGCGCGTGGCTGAACCGCCCTGACGCAGCTGTTGCGAGTAGGATTGCTGATCTGCTTCGAGAAACCGACGTGTCGTACTTGAACCTTAGGGAGGATGCTGGGGCAGTTCCATACGGTTATGGAGAACCTTTAAAACTTCACGATGTAGTTCTTCATTACAATCCAGACGCCTTTGAGTACAATAGTCCAGGTCCTATTAAGGGGCAAGTGCCTGTTAGTAGTACGGTAGCTGACAAGAAGCCCTTCAAGCCTAGTATACCTATACTAAAGCCTTCTCAAAGGGACTATTCCGTATCTGACAATAATCAGCTCAATGATACTCCTCCTAATGACATAACTACACCATCTTCTAGAAAACCCAAATCCAAGAAAAAAGCTAGTGATGTTATGTCATCAGTGGGACCAGATTCTGCTGAAATTCAAGATGCTACCATCAGAAGCTTTTGTGAGTTGGTGGAGGAGTTCTGTGGCAGAGCTGAAATTATCAGTGATGATCGGGAAGAAACTGAGTGGTTATCTATGCCTCTTTCGGATCTTAGACTGCTTGCAAATGAAATAATGTCTCTTCGAGCAAAGAAGCTTCTACATTTGATTCCTGCAGATACTTTTGTTAGATTGTTAAGGATTTTAGATCATCAGATACATCGAGCTGAAGGCTTGACTATAATTGAATATGAGCAGTCAAATTCAGATGTGGTTTCATCAATACAATGTGCACTGGAGGCCATTCACGCAGCATTAGCAGTAATGGCTCATAACCAGATGCCAAAACAACTATATAAAGAAGAGATCATTGAAAGGATTCTGGAGTTTTCCAGGCATCAGATAATGGATGTTATGTGTGCTTATGATCCATCTTATCGTGCTTTGCATAGGCCAAGTCAAAATGGGTCGCTTGAAGTTGATGAAGATGAAGAGCCTGATGCTGAATTTGGCTCAGCAAGCAAGAAGAGACGCAGTATCAAGACCGTGAAAGTGCAGAAATCCTCATTTAACAGGGTCTCTGCTGCTGTGAATAATATTCTTCAGAAAATGTGCACCATTCTTGGTTTAATCAAGGATTTGTTGTTAATAGAAAGGTTGTCAGATGGTTGTATCTTACAACTAGTGAAAACATGCTTCACAACATTTATGGTGGATAATATCCAGCTCTTGCAACTGAAGGCAATGGGTTTGATCAGTGGGATATTCTATTCATACACTCAGCATCGCACATATGTGATAGATGAATTAATTCAGTTGCTTTGGAAGTTGCCAGTCTCGAAGCGAGCTTTGAGAGCATATCACCTACCTGACGAAGAGCAAAGGCAGATTCAGATGATCACTGCTTTGCTGATTCAGTTGGTTCACTATAGTGCAAACCTTCCTGAATCATTAAGGCAGGAATCAAGTAGTAATTCTATCTTAGAACTCTCTGTCAATGCCGATTATCCAACTAAAGGTCACGAGGCAGCAACAGAGGCATGTTGTCACTTTTGGACTCGTGTACTTCAGCGCTTTGCTGGTGCAAAGACTCAGGAAGCTTCAGAGTTGAAAGTAATGATGGAGAATCTTGTTACCGATCTCTTGACAACATTGAATTTACCTGAATATCCAGCTTCAGCTCCTATTCTGGAGGTTCTTTGTGTCTTGTTACTCCAGAATGCTGGGCTGAAATCTAAAGACGTAGCCGCACGTTCAATGGCTATAGACCTTCTCGGCACAATTGCTGCGAGATTGAAGCGTGATTCTCTCCTTTGCAGTAGGGACAAAATCTGGATACTTCAGGAGTTGGTTAGTGACGATGGGGTTGATCAGACATATCCAAGAGATGCATGCTCAGTTTGTTTAGATGGTAGGGTTGAAAAAAATGTCTTTGTTTGTCAAGGTTGTCAGAGAATGTTTCATGCTGATTGTATGGGAGTGAGAGAATATGAAGTTCCAAATCGAAGTTGGCACTGTCAGATATGCCTCTGCAGGAAGCAACTTCTTGTTTTACAATCCTACTGTAAGTCACAATGCAAAGATGAAGGAATAAAGGATCGAAGTCGTTCAGGAAGAAACACTGAAGTTGCGTTTTCAATTACGAAGCCTGAGGTAGTCCAACAGATGCTTTTGAATTACCTTCAAGATACCACATCAGCTGATGATGGGCATCTCTTTGTTCGCTGGTTTTATCTCTTGTTGTGGTACAAGGATGATTCGAAATCACAGCAGAAGTTCATGTACTATCTTGCTAGACTGAAATCAAAAGAAATAGTTCGGGATTCTGGGACTGGTTTTTCATTATTGACAAGGGATTCGGTCAAGAAAATTACTTTAGCATTGGCACAAAAAAACTCTTTCTCCAGAGGGTTTGATAAGATTCTTCATTTGCTTCTGGCAAGCTTAATGGAGAATTCTCCTGTAATCAGGGCCAAGGCTTTACGAGCGGTCAGTATCATTGTAGAAGCTGATCCGCAGGTTTTAGGAGACAAACGCGTTCAATCAGCTGTTGAAGGAAGGTTTTGTGATTCCGCAATTTCCGCTAGAGAAGCAGCACTGGAGCTTGTAGGAAGGCATATTGCTTCTCATCCTGATGTTGGTTTGAAGTATTTTGAGAAGGTTGCTGAGAGAATTAAAGATACTGGAGTAAGTGTTCGAAAGCGATCTATCAAAATCATACGAGATATGTGCGTTTCAAATGCAAACTTCTCCGAATTTACAAGTGCTTGCATTGCTATCATCTCCCGTATTAGTGACGATGAATCAAGCATACAGGATATTGTTTGCAAGACATTTTATGAGTTCTGGTTTGAGGAACCTACTGGTTCACAAACTCAATTCTTTGGAGATGGTAGCTCTGTTCCATTGGAGGTGGCTAAAAAAACTGAACAGATCGTTGAGATGTTGAGGAGGATGCCAAGTCACCAACCTCTTGTGACAGTCATTAAACGCAACTTGGCCCTTGATTTTTTTCCACAATCAGCTAAAGCGGTTGGGATCAACCCTTCCTCACTTGCATCAGTACGCAAGCGCTGTGAGTTAATGTGCAAGTGCTTACTGGAAAAGATATTGCAGGTAGAGGAAATGAATATCCAGGAGGTAGAGAGGCGGGCCCTTCCGTATGTGCTAGCTTTGCATGCATTCTGTGTTGTGGATCCAAAGCTTTGTGCACCGGCTTCTGACCCTTCACAGTTTGTGGTCACTCTTCAGCCTTATCTAAAGAGTCAGGCTGATAATCGAGTGGTTGCACAATTAGTGGAGAGTATAATCTTTATAATTGATGCTGTTCTTCCTTTGGTAAGAAAGTTACCGCGAGCTGTTGTGGAAGAACTTGAACAGGACTTAAAGAACATGATTGTTCGGCATTCCTTCTTGACTGTTGTTCATGCTTGCATAAAGTGCCTCTGTGCTGTGAGTAAAGTGGCAGGAAAAGGTGCTGCTATAGTGGAGAATCTTATCCAGGTGTTCTTCAAACGTTTGGATGCTCAGGCAGTAGATAACAAGCAGCAAGTGGGGCGGTCTCTTTTCTGTCTCGGATTGCTTATCCGTTATGGCAACTCTTTGCCCAGTAACTCCGACAAAACAATTGATGTTGTTAGCAGTCTCAGCTTGTTTAAAAAATATCTTCTAGTGGAGGATTTTGTAATAAAGGTTCGGTCATTGCAGGCATTGGGCTTTGTTCTAATAGCTAGGCCCAAATATATGTTGGAAAAAGATATTGGGAAGATAATAGAGGCAACATTCTCTTCTAGCTCTGATGTTCGTCTTAGGATGCAAGCATTGCAAAACATGTACGACTATCTTCTTGATGCCGAAAGTCAAATGGGAACAGATGCGGCTAGTGATAATGTGATTCATTATAATGTGGAAGGCGGCAATGCTGTATCTGTTGCTGCGGGTGCTGGAGATACTAATATCTGTGGTGGTATAGTTCAGTTGTATTGGGATAACATGTTGGCAAGATGTTTAGACCTTAATGAACAAGTTCGCCAATCTGCAATTAAGATTGTTGAAGTTGTACTGCGGCAAGGGCTCGTACATCCTATTACATGCGTTCCCTACCTTATTGCTCTCGAAACAGATCCACTGGAGGCCAATTCGAAGTTAGCTCATCATTTACTGATGCAAATGAATGAAAAGTACCCTGCATTTTTCGAAAGCCGCCTGGGAGATGGCCTTCAAATGTCTTTTACTTTCATACAGTCCATTAGTACTAGTTCTGAACACGAGAACAAAAAAGTTCAAGCAAAGGCTTCTGGAAATGCAAAGGGAAAATCTGATAGTGTTTCTCTCGCACAAGGCAGACTTGGGGTTTCTCGAATCTACAAGCTTATTCGTGCTAACCGAACTTCAAGAAACAAATTTATGTCTTCGATTGTTCGCAAGTTTGATAGTGCTAGCTGGACACCTTCAGTAGTCCCTTTCCTGATGTACTGCACAGAAATACTCGCCTTGCTACCCTTCACAACACCTGATGAACCGCTTTATTTGGTCTATTCTATAAATAGAGTAGTACAAGTTAGGGCTGGGGCACTCGAAGCAAAGCTGAAAGCTCTGACTTTGCATTTGTTACAAAGAGGTGCATCTCATGGCAACGGCATAATTGAAGATGGCTTAGCTGCTCAGCCTCTTACGGGAGGTATGGCATCATTGGACTTGAATGGGACAGTTGAACAAGAACCAGTGTTCCAACCTGTTACAAATTATATGTCAACGGAGTGGAATGGTACATTACGACAAGAGCCAGCATATCAGTCTGTCTCCAATCAAGCTAACCCGTATGAGGCAAACATGCATGTTACGGGCTCTAGCAGTTCCGGTGGCTTCTCAATAGATGACGTACAGAAATTCCAGGCGGACTGCCTTGCAGCTATTGCAGTGCAGCTTCTTTTGAAACTAAAGAGACACCTAAAAATTGTGTATAGCCTGAATGATGCAAGGTGCCAGGCTTTTTCTCCGGGTGAACCCCCTAAACCGGGAGAAGTACTCTCGAGGCAGAACGTCCCTTTTGACCTCAGTGATACGCACACCAATTTGCCTACAACTTATCAAGAATTGGTACAAAGATATCAGGAATTCAAGAATGCATTGAGGGAGGATACGATTGATTTCTCTACTTACACTGCAAACATCAAGAGAAAACGCCCGGCCCCCAGAAAAGGACGGAAATCTGTGGCTGGAGACGATGAGGGCGAAGATGATGATGAGGATTATTCAGGTGGGGCTCGGAGGCCGAGTAATATTGGAAGGAGAGGTGGCTACAGTATCAGAAGTCGACAGCGGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000070 GO:0000122 GO:0000278 GO:0000280 GO:0000428 GO:0000819 GO:0001654 GO:0001655 GO:0001656 GO:0001822 GO:0003006 GO:0003007 GO:0003008 GO:0003151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007064 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0007423 GO:0007507 GO:0007548 GO:0007600 GO:0007605 GO:0008022 GO:0008104 GO:0008150 GO:0009314 GO:0009628 GO:0009653 GO:0009790 GO:0009887 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010165 GO:0010171 GO:0010212 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010720 GO:0016043 GO:0016591 GO:0019219 GO:0019222 GO:0019899 GO:0019904 GO:0022008 GO:0022402 GO:0022414 GO:0030154 GO:0030326 GO:0030334 GO:0030335 GO:0030880 GO:0031056 GO:0031058 GO:0031063 GO:0031065 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032039 GO:0032116 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033044 GO:0033365 GO:0033554 GO:0034085 GO:0034086 GO:0034088 GO:0034502 GO:0034613 GO:0035107 GO:0035108 GO:0035112 GO:0035113 GO:0035115 GO:0035136 GO:0035239 GO:0035261 GO:0035295 GO:0040007 GO:0040008 GO:0040012 GO:0040017 GO:0042471 GO:0042634 GO:0042826 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043388 GO:0043583 GO:0044093 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045597 GO:0045892 GO:0045934 GO:0045995 GO:0047485 GO:0048285 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048546 GO:0048557 GO:0048562 GO:0048565 GO:0048566 GO:0048568 GO:0048589 GO:0048592 GO:0048598 GO:0048608 GO:0048638 GO:0048699 GO:0048731 GO:0048736 GO:0048806 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0050896 GO:0050954 GO:0051094 GO:0051098 GO:0051099 GO:0051101 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051253 GO:0051270 GO:0051272 GO:0051276 GO:0051641 GO:0051716 GO:0051960 GO:0051962 GO:0055029 GO:0055123 GO:0060065 GO:0060173 GO:0060255 GO:0060284 GO:0060322 GO:0060323 GO:0060324 GO:0060325 GO:0061008 GO:0061010 GO:0061038 GO:0061458 GO:0061695 GO:0065007 GO:0065009 GO:0070013 GO:0070087 GO:0070727 GO:0071168 GO:0071214 GO:0071478 GO:0071479 GO:0071481 GO:0071840 GO:0071921 GO:0072001 GO:0072359 GO:0080090 GO:0090311 GO:0090312 GO:0090596 GO:0090694 GO:0098813 GO:0104004 GO:0140014 GO:1902275 GO:1902494 GO:1902679 GO:1903047 GO:1903506 GO:1903507 GO:1905269 GO:1990234 GO:2000026 GO:2000112 GO:2000113 GO:2000145 GO:2000147 GO:2001141 GO:2001222 GO:2001224 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko04111 map04111
Pfam Domains
Protein Families
Cohesin_HEAT Nipped-B_C PHD

Protein Analysis

1808

Amino Acids

201.61

Weight (kDa)

7.18

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 663 - 707 4.5e-06 PHD-finger
Cohesin_HEAT PF12765 860 - 901 2.8e-12 HEAT repeat associated with sister chromatid cohesion
Nipped-B_C PF12830 1352 - 1549 1.3e-52 Sister chromatid cohesion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010273)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15540 AT5G15540
fragaria_vesca FvH4_6g47080 FvH4_6g47080 FvH4_6g47080
malus_domestica MD09G1064300.v1.1 MD17G1057600.v1.1
prunus_persica Prupe.3G256700_v2.0.a1
pyrus_communis pycom111g05400 pycom17g05770
rosa_chinensis RchiOBHm_Chr2g0166131
rosa_laevigata RLG00000021588
rosa_multiflora Rmu_sc0006218.1_g000028
rosa_roxburghii Rroxscaffold_2G00085180
rosa_rugosa Rorug02G0520500 Rorug02G0520600
rosa_samantha Rh2AG586600 Rh2BG598400 Rh2CG569100 Rh2DG608300
rosa_wichuraiana Rw2G048900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3410, 3996
Acc36I ACCTGC 2 cut(s) 2602, 3199
AccB1I GGYRCC 1 cut(s) 5076
AccBSI CCGCTC 1 cut(s) 2582
AccI GTMKAC 2 cut(s) 427, 5410
AccII CGCG 4 cut(s) 89, 176, 2631, 3448
AclI AACGTT 1 cut(s) 3610
AclWI GGATC 8 cut(s) 711, 1016, 2199, 2600, 3140, 3284, 3297, 4211
AcsI RAATTY 9 cut(s) 588, 856, 1100, 1750, 2548, 2849, 4496, 4982, 5210
AcyI GRCGYC 1 cut(s) 339
AfeI AGCGCT 2 cut(s) 1667, 3167
AfiI CCNNNNNNNGG 9 cut(s) 262, 371, 1610, 2732, 4769, 4918, 5110, 5111, 5294
AflIII ACRYGT 4 cut(s) 240, 2629, 3924, 4086
AhlI ACTAGT 2 cut(s) 1276, 4349
AjiI CACGTC 1 cut(s) 241
AjnI CCWGG 7 cut(s) 358, 986, 3228, 3594, 4299, 4986, 5079
AjuI GAANNNNNNNTTGG 2 cut(s) 3837, 3869
AleI CACNNNNGTG 2 cut(s) 1388, 1524
AloI GAACNNNNNNTCC 4 cut(s) 2772, 2804, 4806, 4838
Alw21I GWGCWC 6 cut(s) 29, 35, 78, 889, 1211, 3307
Alw44I GTGCAC 4 cut(s) 74, 885, 1207, 3303
AlwI GGATC 8 cut(s) 711, 1016, 2199, 2600, 3140, 3284, 3297, 4211
AlwNI CAGNNNCTG 1 cut(s) 581
Ama87I CYCGRG 2 cut(s) 28, 5125
Aor51HI AGCGCT 2 cut(s) 1667, 3167
ApaI GGGCCC 1 cut(s) 3250
ApaLI GTGCAC 4 cut(s) 74, 885, 1207, 3303
ApoI RAATTY 9 cut(s) 588, 856, 1100, 1750, 2548, 2849, 4496, 4982, 5210
ArsI GACNNNNNNTTYG 6 cut(s) 2434, 2466, 4684, 4716, 4829, 4861
AseI ATTAAT 1 cut(s) 1403
Asp700I GAANNNNTTC 6 cut(s) 413, 972, 1404, 2200, 2409, 4119
AspLEI GCGC 5 cut(s) 91, 131, 176, 1668, 3168
AspS9I GGNCC 8 cut(s) 362, 572, 2565, 3094, 3246, 3247, 3828, 5284
AsuC2I CCSGG 3 cut(s) 5094, 5112, 5282
AsuHPI GGTGA 4 cut(s) 131, 1448, 3050, 5108
AsuII TTCGAA 4 cut(s) 2354, 2791, 4235, 4290
AsuNHI GCTAGC 2 cut(s) 3262, 4532
AvaI CYCGRG 2 cut(s) 28, 5125
AvaII GGWCC 2 cut(s) 362, 572
AxyI CCTNAGG 2 cut(s) 257, 2244
BaeGI GKGCMC 6 cut(s) 78, 889, 1211, 3250, 3307, 4669
BaeI ACNNNNGTAYC 4 cut(s) 765, 798, 2766, 2799
BamHI GGATCC 1 cut(s) 3289
BanI GGYRCC 1 cut(s) 5076
BanII GRGCYC 6 cut(s) 29, 35, 3250, 4170, 4943, 5362
BarI GAAGNNNNNNTAC 2 cut(s) 2360, 2392
BauI CACGAG 3 cut(s) 1616, 1653, 4361
BbsI GAAGAC 1 cut(s) 4497
Bbv12I GWGCWC 6 cut(s) 29, 35, 78, 889, 1211, 3307
BceAI ACGGC 2 cut(s) 64, 4747
BcgI CGANNNNNNTGC 2 cut(s) 2805, 2839
BciT130I CCWGG 7 cut(s) 360, 988, 3230, 3596, 4301, 4988, 5081
BciVI GTATCC 2 cut(s) 1923, 5227
BclI TGATCA 4 cut(s) 136, 1347, 1491, 1963
BcnI CCSGG 3 cut(s) 5094, 5112, 5282
BcuI ACTAGT 2 cut(s) 1276, 4349
BfmI CTRYAG 6 cut(s) 768, 1522, 1857, 2125, 3576, 5395
BfoI RGCGCY 2 cut(s) 1669, 3169
BfuAI ACCTGC 2 cut(s) 2602, 3199
BfuI GTATCC 2 cut(s) 1923, 5227
BlpI GCTNAGC 3 cut(s) 1108, 4750, 4758
BmcAI AGTACT 2 cut(s) 4348, 5121
Bme18I GGWCC 2 cut(s) 362, 572
BmeT110I CYCGRG 2 cut(s) 28, 5125
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 8 cut(s) 362, 572, 2565, 3094, 3246, 3247, 3828, 5284
BmiI GGNNCC 6 cut(s) 573, 2952, 3248, 3291, 5078, 5286
BmrI ACTGGG 1 cut(s) 3696
BmtI GCTAGC 2 cut(s) 3266, 4536
BmuI ACTGGG 1 cut(s) 3696
BpiI GAAGAC 1 cut(s) 4497
BplI GAGNNNNNCTC 2 cut(s) 5106, 5138
Bpu10I CCTNAGC 1 cut(s) 3621
Bpu1102I GCTNAGC 3 cut(s) 1108, 4750, 4758
Bpu14I TTCGAA 4 cut(s) 2354, 2791, 4235, 4290
BpuMI CCSGG 3 cut(s) 5094, 5112, 5282
BsaBI GATNNNNATC 1 cut(s) 2304
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 2 cut(s) 1177, 3660
BsaJI CCNNGG 2 cut(s) 2568, 4300
BsaWI WCCGGW 2 cut(s) 10, 4952
BsaXI ACNNNNNCTCC 6 cut(s) 2480, 2510, 2772, 2802, 5356, 5386
Bsc4I CCNNNNNNNGG 9 cut(s) 262, 371, 1610, 2732, 4769, 4918, 5110, 5111, 5294
Bse118I RCCGGY 2 cut(s) 115, 3307
Bse21I CCTNAGG 2 cut(s) 257, 2244
Bse3DI GCAATG 5 cut(s) 1344, 2865, 3794, 3914, 4018
Bse8I GATNNNNATC 1 cut(s) 2304
BseBI CCWGG 7 cut(s) 360, 988, 3230, 3596, 4301, 4988, 5081
BseDI CCNNGG 2 cut(s) 2568, 4300
BseGI GGATG 8 cut(s) 271, 1009, 2353, 2722, 3054, 3622, 3912, 4174
BseJI GATNNNNATC 1 cut(s) 2304
BseLI CCNNNNNNNGG 9 cut(s) 262, 371, 1610, 2732, 4769, 4918, 5110, 5111, 5294
BseMI GCAATG 5 cut(s) 1344, 2865, 3794, 3914, 4018
BseRI GAGGAG 3 cut(s) 483, 644, 3058
BseSI GKGCMC 6 cut(s) 78, 889, 1211, 3250, 3307, 4669
BseYI CCCAGC 3 cut(s) 269, 1817, 4661
BsgI GTGCAG 3 cut(s) 1169, 4555, 5036
Bsh1236I CGCG 4 cut(s) 89, 176, 2631, 3448
BshNI GGYRCC 1 cut(s) 5076
BsiHKAI GWGCWC 6 cut(s) 29, 35, 78, 889, 1211, 3307
BsiHKCI CYCGRG 2 cut(s) 28, 5125
BsiSI CCGG 9 cut(s) 11, 36, 116, 145, 3308, 4953, 5093, 5111, 5282
BslFI GGGAC 7 cut(s) 467, 585, 1931, 2437, 4538, 4813, 5123
BslI CCNNNNNNNGG 9 cut(s) 262, 371, 1610, 2732, 4769, 4918, 5110, 5111, 5294
BsmBI CGTCTC 2 cut(s) 1117, 5310
BsmFI GGGAC 7 cut(s) 467, 585, 1931, 2437, 4538, 4813, 5123
BsmI GAATGC 4 cut(s) 1819, 3275, 3499, 5224
Bso31I GGTCTC 2 cut(s) 1177, 3660
BsoBI CYCGRG 2 cut(s) 28, 5125
Bsp119I TTCGAA 4 cut(s) 2354, 2791, 4235, 4290
Bsp120I GGGCCC 1 cut(s) 3246
Bsp1720I GCTNAGC 3 cut(s) 1108, 4750, 4758
BspFNI CGCG 4 cut(s) 89, 176, 2631, 3448
BspLI GGNNCC 6 cut(s) 573, 2952, 3248, 3291, 5078, 5286
BspMAI CTGCAG 2 cut(s) 772, 2129
BspMI ACCTGC 2 cut(s) 2602, 3199
BspOI GCTAGC 2 cut(s) 3266, 4536
BspPI GGATC 8 cut(s) 711, 1016, 2199, 2600, 3140, 3284, 3297, 4211
BspQI GCTCTTC 2 cut(s) 1080, 1464
BspT104I TTCGAA 4 cut(s) 2354, 2791, 4235, 4290
BspT107I GGYRCC 1 cut(s) 5076
BspTNI GGTCTC 2 cut(s) 1177, 3660
BsrBI CCGCTC 1 cut(s) 2582
BsrDI GCAATG 5 cut(s) 1344, 2865, 3794, 3914, 4018
BsrFI RCCGGY 2 cut(s) 115, 3307
BssAI RCCGGY 2 cut(s) 115, 3307
BssECI CCNNGG 2 cut(s) 2568, 4300
BssNAI GTATAC 1 cut(s) 428
BssNI GRCGYC 1 cut(s) 339
BssSI CACGAG 3 cut(s) 1616, 1653, 4361
BssT1I CCWWGG 1 cut(s) 2568
Bst1107I GTATAC 1 cut(s) 428
Bst2BI CACGAG 3 cut(s) 1616, 1653, 4361
Bst2UI CCWGG 7 cut(s) 360, 988, 3230, 3596, 4301, 4988, 5081
Bst6I CTCTTC 7 cut(s) 741, 951, 1080, 1115, 1464, 3345, 3886
BstACI GRCGYC 1 cut(s) 339
BstAPI GCANNNNNTGC 4 cut(s) 203, 3534, 3916, 5009
BstBI TTCGAA 4 cut(s) 2354, 2791, 4235, 4290
BstENI CCTNNNNNAGG 1 cut(s) 260
BstF5I GGATG 8 cut(s) 271, 1009, 2353, 2722, 3054, 3622, 3912, 4174
BstFNI CGCG 4 cut(s) 89, 176, 2631, 3448
BstH2I RGCGCY 2 cut(s) 1669, 3169
BstHHI GCGC 5 cut(s) 91, 131, 176, 1668, 3168
BstNI CCWGG 7 cut(s) 360, 988, 3230, 3596, 4301, 4988, 5081
BstNSI RCATGY 9 cut(s) 1290, 1638, 1989, 3275, 3928, 4090, 4188, 4930, 4934
BstSFI CTRYAG 6 cut(s) 768, 1522, 1857, 2125, 3576, 5395
BstSLI GKGCMC 6 cut(s) 78, 889, 1211, 3250, 3307, 4669
BstUI CGCG 4 cut(s) 89, 176, 2631, 3448
BstV2I GAAGAC 1 cut(s) 4497
BstX2I RGATCY 3 cut(s) 703, 3289, 4216
BstXI CCANNNNNNTGG 1 cut(s) 1817
BstYI RGATCY 3 cut(s) 703, 3289, 4216
BstZ17I GTATAC 1 cut(s) 428
Bsu36I CCTNAGG 2 cut(s) 257, 2244
BsuI GTATCC 2 cut(s) 1923, 5227
BtgZI GCGATG 2 cut(s) 93, 1364
BtrI CACGTC 1 cut(s) 241
BtsCI GGATG 8 cut(s) 271, 1009, 2353, 2722, 3054, 3622, 3912, 4174
BtsI GCAGTG 3 cut(s) 1494, 5019, 5256
BveI ACCTGC 2 cut(s) 2602, 3199
CaiI CAGNNNCTG 1 cut(s) 581
CfoI GCGC 5 cut(s) 91, 131, 176, 1668, 3168
Cfr10I RCCGGY 2 cut(s) 115, 3307
Cfr13I GGNCC 8 cut(s) 362, 572, 2565, 3094, 3246, 3247, 3828, 5284
CseI GACGC 3 cut(s) 203, 347, 1134
DraI TTTAAA 2 cut(s) 303, 3751
Eam1104I CTCTTC 7 cut(s) 741, 951, 1080, 1115, 1464, 3345, 3886
EarI CTCTTC 7 cut(s) 741, 951, 1080, 1115, 1464, 3345, 3886
EciI GGCGGA 1 cut(s) 5006
Ecl136II GAGCTC 2 cut(s) 27, 33
Eco130I CCWWGG 1 cut(s) 2568
Eco24I GRGCYC 6 cut(s) 29, 35, 3250, 4170, 4943, 5362
Eco31I GGTCTC 2 cut(s) 1177, 3660
Eco32I GATATC 1 cut(s) 5204
Eco47I GGWCC 2 cut(s) 362, 572
Eco47III AGCGCT 2 cut(s) 1667, 3167
Eco53kI GAGCTC 2 cut(s) 27, 33
Eco81I CCTNAGG 2 cut(s) 257, 2244
Eco88I CYCGRG 2 cut(s) 28, 5125
EcoICRI GAGCTC 2 cut(s) 27, 33
EcoNI CCTNNNNNAGG 1 cut(s) 260
EcoO109I RGGNCCY 2 cut(s) 362, 3247
EcoRI GAATTC 2 cut(s) 2548, 5210
EcoRII CCWGG 7 cut(s) 358, 986, 3228, 3594, 4299, 4986, 5079
EcoRV GATATC 1 cut(s) 5204
EcoT14I CCWWGG 1 cut(s) 2568
EcoT22I ATGCAT 4 cut(s) 1987, 3277, 4932, 5224
EcoT38I GRGCYC 6 cut(s) 29, 35, 3250, 4170, 4943, 5362
ErhI CCWWGG 1 cut(s) 2568
Esp3I CGTCTC 2 cut(s) 1117, 5310
FalI AAGNNNNNCTT 4 cut(s) 398, 430, 4425, 4457
FaqI GGGAC 7 cut(s) 467, 585, 1931, 2437, 4538, 4813, 5123
FauI CCCGC 2 cut(s) 3237, 4024
FauNDI CATATG 1 cut(s) 1387
FbaI TGATCA 4 cut(s) 136, 1347, 1491, 1963
FblI GTMKAC 2 cut(s) 427, 5410
FokI GGATG 8 cut(s) 278, 1016, 2360, 2709, 3061, 3629, 3919, 4161
FriOI GRGCYC 6 cut(s) 29, 35, 3250, 4170, 4943, 5362
GlaI GCGC 5 cut(s) 90, 130, 175, 1667, 3167
GsaI CCCAGC 3 cut(s) 273, 1821, 4665
HaeII RGCGCY 2 cut(s) 1669, 3169
HapII CCGG 9 cut(s) 11, 36, 116, 145, 3308, 4953, 5093, 5111, 5282
HgaI GACGC 3 cut(s) 203, 347, 1134
HhaI GCGC 5 cut(s) 91, 131, 176, 1668, 3168
Hin1I GRCGYC 1 cut(s) 339
Hin6I GCGC 5 cut(s) 89, 129, 174, 1666, 3166
HinP1I GCGC 5 cut(s) 89, 129, 174, 1666, 3166
HincII GTYRAC 2 cut(s) 4848, 5411
HindII GTYRAC 2 cut(s) 4848, 5411
HindIII AAGCTT 6 cut(s) 610, 749, 1692, 2536, 3296, 4463
HpaII CCGG 9 cut(s) 11, 36, 116, 145, 3308, 4953, 5093, 5111, 5282
HphI GGTGA 4 cut(s) 131, 1448, 3050, 5108
Hpy99I CGWCG 2 cut(s) 158, 242
HpyCH4IV ACGT 6 cut(s) 240, 1836, 1846, 3610, 4974, 5136
HpySE526I ACGT 6 cut(s) 240, 1836, 1846, 3610, 4974, 5136
Hsp92I GRCGYC 1 cut(s) 339
HspAI GCGC 5 cut(s) 89, 129, 174, 1666, 3166
Ksp22I TGATCA 4 cut(s) 136, 1347, 1491, 1963
LguI GCTCTTC 2 cut(s) 1080, 1464
LmnI GCTCC 3 cut(s) 38, 1780, 2695
MaeII ACGT 6 cut(s) 240, 1836, 1846, 3610, 4974, 5136
MbiI CCGCTC 1 cut(s) 2582
MfeI CAATTG 2 cut(s) 1875, 3720
MflI RGATCY 3 cut(s) 703, 3289, 4216
MluI ACGCGT 1 cut(s) 2629
MlyI GAGTC 4 cut(s) 172, 1645, 1678, 3367
MmeI TCCRAC 7 cut(s) 90, 146, 1628, 2278, 3735, 3822, 4849
Mph1103I ATGCAT 4 cut(s) 1987, 3277, 4932, 5224
MroXI GAANNNNTTC 6 cut(s) 413, 972, 1404, 2200, 2409, 4119
MslI CAYNNNNRTG 4 cut(s) 1388, 1524, 2727, 3996
MspA1I CMGCKG 4 cut(s) 200, 2294, 2642, 5416
MspI CCGG 9 cut(s) 11, 36, 116, 145, 3308, 4953, 5093, 5111, 5282
MunI CAATTG 2 cut(s) 1875, 3720
Mva1269I GAATGC 4 cut(s) 1819, 3275, 3499, 5224
MvaI CCWGG 7 cut(s) 360, 988, 3230, 3596, 4301, 4988, 5081
MvnI CGCG 4 cut(s) 89, 176, 2631, 3448
NciI CCSGG 3 cut(s) 5094, 5112, 5282
NdeI CATATG 1 cut(s) 1387
NheI GCTAGC 2 cut(s) 3262, 4532
NlaIV GGNNCC 6 cut(s) 573, 2952, 3248, 3291, 5078, 5286
NmeAIII GCCGAG 4 cut(s) 20, 186, 1848, 5394
NmuCI GTSAC 9 cut(s) 16, 1613, 1640, 1949, 2163, 2888, 3056, 3070, 3334
NsiI ATGCAT 4 cut(s) 1987, 3277, 4932, 5224
NspI RCATGY 9 cut(s) 1290, 1638, 1989, 3275, 3928, 4090, 4188, 4930, 4934
NspV TTCGAA 4 cut(s) 2354, 2791, 4235, 4290
OliI CACNNNNGTG 2 cut(s) 1388, 1524
PaeI GCATGC 2 cut(s) 1989, 3275
PaeR7I CTCGAG 2 cut(s) 28, 5125
PciI ACATGT 2 cut(s) 3924, 4086
PciSI GCTCTTC 2 cut(s) 1080, 1464
PcsI WCGNNNNNNNCGW 2 cut(s) 4477, 5325
PctI GAATGC 4 cut(s) 1819, 3275, 3499, 5224
PdmI GAANNNNTTC 6 cut(s) 413, 972, 1404, 2200, 2409, 4119
PfoI TCCNGGA 1 cut(s) 3228
PleI GAGTC 4 cut(s) 171, 1645, 1678, 3366
PpsI GAGTC 4 cut(s) 171, 1645, 1678, 3366
PpuMI RGGWCCY 1 cut(s) 362
PscI ACATGT 2 cut(s) 3924, 4086
PshBI ATTAAT 1 cut(s) 1403
PsiI TTATAA 2 cut(s) 3410, 3996
Psp124BI GAGCTC 2 cut(s) 29, 35
Psp1406I AACGTT 1 cut(s) 3610
Psp5II RGGWCCY 1 cut(s) 362
Psp6I CCWGG 7 cut(s) 358, 986, 3228, 3594, 4299, 4986, 5079
PspFI CCCAGC 3 cut(s) 269, 1817, 4661
PspGI CCWGG 7 cut(s) 358, 986, 3228, 3594, 4299, 4986, 5079
PspN4I GGNNCC 6 cut(s) 573, 2952, 3248, 3291, 5078, 5286
PspOMI GGGCCC 1 cut(s) 3246
PspPI GGNCC 8 cut(s) 362, 572, 2565, 3094, 3246, 3247, 3828, 5284
PspPPI RGGWCCY 1 cut(s) 362
PspXI VCTCGAGB 1 cut(s) 28
PsrI GAACNNNNNNTAC 2 cut(s) 3771, 3803
PstI CTGCAG 2 cut(s) 772, 2129
PstNI CAGNNNCTG 1 cut(s) 581
PsuI RGATCY 3 cut(s) 703, 3289, 4216
PvuII CAGCTG 3 cut(s) 200, 2294, 2642
RseI CAYNNNNRTG 4 cut(s) 1388, 1524, 2727, 3996
SacI GAGCTC 2 cut(s) 29, 35
SalI GTCGAC 1 cut(s) 5409
SapI GCTCTTC 2 cut(s) 1080, 1464
Sau96I GGNCC 8 cut(s) 362, 572, 2565, 3094, 3246, 3247, 3828, 5284
ScaI AGTACT 2 cut(s) 4348, 5121
SchI GAGTC 4 cut(s) 172, 1645, 1678, 3367
SfcI CTRYAG 6 cut(s) 768, 1522, 1857, 2125, 3576, 5395
Sfr274I CTCGAG 2 cut(s) 28, 5125
SfuI TTCGAA 4 cut(s) 2354, 2791, 4235, 4290
SinI GGWCC 2 cut(s) 362, 572
SlaI CTCGAG 2 cut(s) 28, 5125
SmiMI CAYNNNNRTG 4 cut(s) 1388, 1524, 2727, 3996
SmlI CTYRAG 2 cut(s) 28, 5125
SmoI CTYRAG 2 cut(s) 28, 5125
SpeI ACTAGT 2 cut(s) 1276, 4349
SphI GCATGC 2 cut(s) 1989, 3275
SspI AATATT 2 cut(s) 1192, 5377
SstI GAGCTC 2 cut(s) 29, 35
StyI CCWWGG 1 cut(s) 2568
TaiI ACGT 6 cut(s) 243, 1839, 1849, 3613, 4977, 5139
TaqII GACCGA 2 cut(s) 2443, 3780
TatI WGTACW 8 cut(s) 348, 1657, 2376, 4153, 4346, 4566, 4648, 5119
TauI GCSGC 7 cut(s) 54, 129, 1844, 3974, 4011, 4162, 4299
TseFI GTSAC 9 cut(s) 16, 1613, 1640, 1949, 2163, 2888, 3056, 3070, 3334
Tsp45I GTSAC 9 cut(s) 16, 1613, 1640, 1949, 2163, 2888, 3056, 3070, 3334
TspGWI ACGGA 5 cut(s) 451, 3243, 3671, 4865, 5314
VneI GTGCAC 4 cut(s) 74, 885, 1207, 3303
VpaK11BI GGWCC 2 cut(s) 362, 572
VspI ATTAAT 1 cut(s) 1403
XagI CCTNNNNNAGG 1 cut(s) 260
XapI RAATTY 9 cut(s) 588, 856, 1100, 1750, 2548, 2849, 4496, 4982, 5210
XbaI TCTAGA 1 cut(s) 521
XceI RCATGY 9 cut(s) 1290, 1638, 1989, 3275, 3928, 4090, 4188, 4930, 4934
XcmI CCANNNNNNNNNTGG 2 cut(s) 2100, 3838
XhoI CTCGAG 2 cut(s) 28, 5125
XmiI GTMKAC 2 cut(s) 427, 5410
XmnI GAANNNNTTC 6 cut(s) 413, 972, 1404, 2200, 2409, 4119
ZrmI AGTACT 2 cut(s) 4348, 5121
Zsp2I ATGCAT 4 cut(s) 1987, 3277, 4932, 5224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.