MD09G1082100.v1.1

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0016903
Physical Location & Seq
Forward (+)
5770846 .. 5772798
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1082100.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGCAATGGAAGAAATGGAAGTCCCAAAACATTTGATGTCCTCTTCAGGGGAGGAACATAAAATCTGTGTCAAAAATGCATTCAGCTGCAGTTTAGATGCAAGAGATAGGAGGCTGGTTTGTGTGACCAGTGGGAATTCTCACTTGGGTTCTAACATAGTTAGGGCTCTATTGGCCCGGGGTTATTTGGTCCGAGTTACCATTCAAAACGAAGTGGATTTTGAGGACATGAAGAAGCTAATGAGAGGTGAAGAGATAAGCCAGTTGGAGTGCGTGGTTGTGGCAAAGATGGGTGATTTGAATAGTCTCTGTGATGCCTTTAGAGGTTGCCATGCAATTTTTCACACCTCCTCTTTTATTGATCCACATGGTATCTCCGGTTATTCGGAAAAAATGGCATTTCTTGAAACTGAAGGGGCAAGAAATGTAATTGAAGCTTGTGGGAGGGCAGCATATGCAAAGAGATGCATCTTCACTTCCTCTCTCCTTGCTGCCATCTGGACAGGTGAAAACTATGAAGAAAAGAAGATCATCGATGAGTATAGCTGGACCAATGTGGATTTCTGCAGAGAAAACAAGCTTTGGTTAGCCCTAGGGAAGACCACAGCAGAGATGGCTTGTTGGAGGAAGTCCAAGGAAATGAAAGTCAATCTTGTTACTGTGTGCCCTGGCCTCCTAATGGATGCATCTCATCCAAATGCTCACATATCCACTTCCATACCATATCTTAAAGGTGGCAAGGCAATGTTCAAACACGGTCGCCTAGCTGTGGAGGACGTGAAGAAGGCGGCTGAGGCGCATGTCTGCGTTTATGAAGCGATGGACCATGGCGCCGGCGGGCGATACCTCAGCTTTGGGAAGGTTGTAACAAAAATGGCTGAAGCCATTGAGTTAGAGAATGGATTGAAGATTCATGGGTTGTTATCAGGAGAAGGACTTGAGGACTTGGGTGAAGAAACAGATGAAGAAGTACAAAGCATGCTTAGCAATGCAAAGCTTGCCAGATTGATTGGAGCTTCACAAAAGTTGTCTTGCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD NAD_binding_10

Protein Analysis

347

Amino Acids

38.17

Weight (kDa)

6.2

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 42 - 182 8.7e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016903)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45521
malus_domestica MD09G1082100.v1.1
prunus_persica Prupe.3G241700_v2.0.a1
pyrus_communis pycom09g00860
rosa_chinensis RchiOBHm_Chr2g0163471
rosa_laevigata RLG00000021404
rosa_multiflora Rmu_co8460151.1_g000001
rosa_roxburghii Rroxscaffold_2G00087290
rosa_rugosa Rorug02G0503000
rosa_samantha Rh2AG570000 Rh2BG582200 Rh2CG552000 Rh2DG592000
rosa_wichuraiana Rw2G047190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 830
AciI CCGC 2 cut(s) 788, 837
AclWI GGATC 1 cut(s) 356
AcsI RAATTY 1 cut(s) 136
AcuI CTGAAG 3 cut(s) 30, 432, 900
AcyI GRCGYC 1 cut(s) 831
AfaI GTAC 1 cut(s) 972
AfiI CCNNNNNNNGG 3 cut(s) 48, 679, 769
AgsI TTSAA 6 cut(s) 206, 301, 407, 434, 751, 907
AjiI CACGTC 1 cut(s) 778
AjnI CCWGG 1 cut(s) 667
AjuI GAANNNNNNNTTGG 4 cut(s) 128, 160, 545, 577
AluBI AGCT 9 cut(s) 87, 238, 437, 546, 580, 767, 852, 997, 1016
AluI AGCT 9 cut(s) 87, 238, 437, 546, 580, 767, 852, 997, 1016
Alw26I GTCTC 1 cut(s) 311
AlwI GGATC 1 cut(s) 356
Ama87I CYCGRG 1 cut(s) 177
AoxI GGCC 2 cut(s) 174, 670
ApeKI GCWGC 3 cut(s) 87, 449, 491
ApoI RAATTY 1 cut(s) 136
AspA2I CCTAGG 1 cut(s) 592
AspLEI GCGC 2 cut(s) 799, 833
AspS9I GGNCC 4 cut(s) 175, 190, 549, 823
AsuC2I CCSGG 2 cut(s) 178, 179
AsuHPI GGTGA 4 cut(s) 260, 305, 518, 962
AvaI CYCGRG 1 cut(s) 177
AvaII GGWCC 3 cut(s) 190, 549, 823
AvrII CCTAGG 1 cut(s) 592
BaeGI GKGCMC 1 cut(s) 668
BanI GGYRCC 1 cut(s) 830
BanII GRGCYC 1 cut(s) 169
BbsI GAAGAC 1 cut(s) 605
BbvCI CCTCAGC 2 cut(s) 792, 848
BbvI GCAGC 3 cut(s) 74, 461, 478
BccI CCATC 4 cut(s) 283, 503, 607, 814
BciT130I CCWGG 1 cut(s) 669
BcnI CCSGG 2 cut(s) 178, 179
BcoDI GTCTC 1 cut(s) 311
BfaI CTAG 2 cut(s) 593, 764
BfmI CTRYAG 2 cut(s) 88, 565
BfoI RGCGCY 1 cut(s) 834
BisI GCNGC 4 cut(s) 88, 450, 492, 789
BlnI CCTAGG 1 cut(s) 592
BlpI GCTNAGC 1 cut(s) 983
BlsI GCNGC 4 cut(s) 89, 451, 493, 790
Bme1390I CCNGG 3 cut(s) 178, 179, 669
Bme18I GGWCC 3 cut(s) 190, 549, 823
BmeT110I CYCGRG 1 cut(s) 177
BmgBI CACGTC 1 cut(s) 778
BmgT120I GGNCC 4 cut(s) 175, 190, 549, 823
BmiI GGNNCC 1 cut(s) 832
BmrFI CCNGG 3 cut(s) 178, 179, 669
BmsI GCATC 6 cut(s) 88, 304, 455, 477, 673, 695
BpiI GAAGAC 1 cut(s) 605
Bpu10I CCTNAGC 2 cut(s) 792, 848
Bpu1102I GCTNAGC 1 cut(s) 983
BpuEI CTTGAG 1 cut(s) 959
BpuMI CCSGG 2 cut(s) 178, 179
Bsa29I ATCGAT 1 cut(s) 534
BsaHI GRCGYC 1 cut(s) 831
BsaJI CCNNGG 6 cut(s) 177, 178, 592, 633, 667, 826
BsaWI WCCGGW 1 cut(s) 377
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 679, 769
Bse118I RCCGGY 1 cut(s) 833
Bse1I ACTGG 2 cut(s) 129, 262
Bse3DI GCAATG 3 cut(s) 12, 750, 994
BseBI CCWGG 1 cut(s) 669
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 6 cut(s) 177, 178, 592, 633, 667, 826
BseGI GGATG 2 cut(s) 688, 691
BseLI CCNNNNNNNGG 3 cut(s) 48, 679, 769
BseMI GCAATG 3 cut(s) 12, 750, 994
BseMII CTCAG 2 cut(s) 783, 862
BseNI ACTGG 2 cut(s) 129, 262
BseRI GAGGAG 1 cut(s) 340
BseSI GKGCMC 1 cut(s) 668
BseXI GCAGC 3 cut(s) 74, 461, 478
Bsh1285I CGRYCG 1 cut(s) 760
BshFI GGCC 2 cut(s) 176, 672
BshNI GGYRCC 1 cut(s) 830
BshVI ATCGAT 1 cut(s) 534
BsiEI CGRYCG 1 cut(s) 760
BsiHKCI CYCGRG 1 cut(s) 177
BsiSI CCGG 3 cut(s) 178, 378, 834
BslFI GGGAC 1 cut(s) 8
BslI CCNNNNNNNGG 3 cut(s) 48, 679, 769
BsmAI GTCTC 1 cut(s) 311
BsmFI GGGAC 1 cut(s) 8
BsmI GAATGC 1 cut(s) 80
BsnI GGCC 2 cut(s) 176, 672
BsoBI CYCGRG 1 cut(s) 177
Bsp1286I GDGCHC 2 cut(s) 169, 668
Bsp143I GATC 2 cut(s) 361, 528
Bsp1720I GCTNAGC 1 cut(s) 983
Bsp19I CCATGG 1 cut(s) 826
BspACI CCGC 2 cut(s) 788, 837
BspANI GGCC 2 cut(s) 176, 672
BspCNI CTCAG 2 cut(s) 784, 861
BspDI ATCGAT 1 cut(s) 534
BspLI GGNNCC 1 cut(s) 832
BspMAI CTGCAG 2 cut(s) 92, 569
BspPI GGATC 1 cut(s) 356
BspT107I GGYRCC 1 cut(s) 830
BsrDI GCAATG 3 cut(s) 12, 750, 994
BsrFI RCCGGY 1 cut(s) 833
BsrI ACTGG 2 cut(s) 129, 262
BssAI RCCGGY 1 cut(s) 833
BssECI CCNNGG 6 cut(s) 177, 178, 592, 633, 667, 826
BssMI GATC 2 cut(s) 361, 528
BssNI GRCGYC 1 cut(s) 831
BssT1I CCWWGG 3 cut(s) 592, 633, 826
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 2 cut(s) 661, 758
Bst6I CTCTTC 2 cut(s) 49, 246
BstACI GRCGYC 1 cut(s) 831
BstAPI GCANNNNNTGC 2 cut(s) 455, 998
BstC8I GCNNGC 4 cut(s) 835, 839, 980, 999
BstDEI CTNAG 3 cut(s) 792, 848, 983
BstDSI CCRYGG 1 cut(s) 826
BstF5I GGATG 2 cut(s) 688, 691
BstH2I RGCGCY 1 cut(s) 834
BstHHI GCGC 2 cut(s) 799, 833
BstKTI GATC 2 cut(s) 364, 531
BstMAI GTCTC 1 cut(s) 311
BstMBI GATC 2 cut(s) 361, 528
BstMCI CGRYCG 1 cut(s) 760
BstMWI GCNNNNNNNGC 6 cut(s) 173, 455, 614, 794, 984, 998
BstNI CCWGG 1 cut(s) 669
BstNSI RCATGY 2 cut(s) 803, 982
BstSCI CCNGG 3 cut(s) 176, 177, 667
BstSFI CTRYAG 2 cut(s) 88, 565
BstSLI GKGCMC 1 cut(s) 668
BstV1I GCAGC 3 cut(s) 74, 461, 478
BstV2I GAAGAC 1 cut(s) 605
Bsu15I ATCGAT 1 cut(s) 534
BsuRI GGCC 2 cut(s) 176, 672
BsuTUI ATCGAT 1 cut(s) 534
BtgI CCRYGG 1 cut(s) 826
BtgZI GCGATG 1 cut(s) 833
BtrI CACGTC 1 cut(s) 778
BtsCI GGATG 2 cut(s) 688, 691
BtsIMutI CAGTG 1 cut(s) 136
Cac8I GCNNGC 4 cut(s) 835, 839, 980, 999
CfoI GCGC 2 cut(s) 799, 833
Cfr10I RCCGGY 1 cut(s) 833
Cfr13I GGNCC 4 cut(s) 175, 190, 549, 823
Cfr9I CCCGGG 1 cut(s) 177
ClaI ATCGAT 1 cut(s) 534
Csp6I GTAC 1 cut(s) 971
CviAII CATG 7 cut(s) 229, 332, 368, 800, 827, 914, 979
CviQI GTAC 1 cut(s) 971
DdeI CTNAG 3 cut(s) 792, 848, 983
DinI GGCGCC 1 cut(s) 832
DpnI GATC 2 cut(s) 363, 530
DpnII GATC 2 cut(s) 361, 528
Eam1104I CTCTTC 2 cut(s) 49, 246
EarI CTCTTC 2 cut(s) 49, 246
Eco130I CCWWGG 3 cut(s) 592, 633, 826
Eco24I GRGCYC 1 cut(s) 169
Eco47I GGWCC 3 cut(s) 190, 549, 823
Eco57I CTGAAG 3 cut(s) 30, 432, 900
Eco88I CYCGRG 1 cut(s) 177
EcoRI GAATTC 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 667
EcoT14I CCWWGG 3 cut(s) 592, 633, 826
EcoT22I ATGCAT 3 cut(s) 82, 470, 688
EcoT38I GRGCYC 1 cut(s) 169
EgeI GGCGCC 1 cut(s) 832
EheI GGCGCC 1 cut(s) 832
ErhI CCWWGG 3 cut(s) 592, 633, 826
FaeI CATG 7 cut(s) 232, 335, 371, 803, 830, 917, 982
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FaqI GGGAC 1 cut(s) 8
FatI CATG 7 cut(s) 228, 331, 367, 799, 826, 913, 978
FauI CCCGC 1 cut(s) 830
FauNDI CATATG 1 cut(s) 454
Fnu4HI GCNGC 4 cut(s) 88, 450, 492, 789
FokI GGATG 2 cut(s) 678, 695
FriOI GRGCYC 1 cut(s) 169
Fsp4HI GCNGC 4 cut(s) 88, 450, 492, 789
FspBI CTAG 2 cut(s) 593, 764
GlaI GCGC 2 cut(s) 798, 832
GluI GCNGC 4 cut(s) 88, 450, 492, 789
HaeII RGCGCY 1 cut(s) 834
HaeIII GGCC 2 cut(s) 176, 672
HapII CCGG 3 cut(s) 178, 378, 834
HhaI GCGC 2 cut(s) 799, 833
Hin1I GRCGYC 1 cut(s) 831
Hin1II CATG 7 cut(s) 232, 335, 371, 803, 830, 917, 982
Hin6I GCGC 2 cut(s) 797, 831
HinP1I GCGC 2 cut(s) 797, 831
HindIII AAGCTT 3 cut(s) 435, 578, 995
HinfI GANTC 1 cut(s) 910
HpaII CCGG 3 cut(s) 178, 378, 834
HphI GGTGA 4 cut(s) 260, 305, 518, 962
Hpy188I TCNGA 2 cut(s) 194, 388
Hpy188III TCNNGA 3 cut(s) 404, 499, 927
HpyAV CCTTC 4 cut(s) 407, 778, 853, 926
HpyCH4III ACNGT 2 cut(s) 661, 758
HpyCH4IV ACGT 1 cut(s) 777
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 455, 614, 794, 984, 998
HpyF3I CTNAG 3 cut(s) 792, 848, 983
HpySE526I ACGT 1 cut(s) 777
Hsp92I GRCGYC 1 cut(s) 831
Hsp92II CATG 7 cut(s) 232, 335, 371, 803, 830, 917, 982
HspAI GCGC 2 cut(s) 797, 831
KasI GGCGCC 1 cut(s) 830
KroI GCCGGC 1 cut(s) 833
KroNI GCCGGC 1 cut(s) 835
Kzo9I GATC 2 cut(s) 361, 528
LmnI GCTCC 1 cut(s) 1013
Lsp1109I GCAGC 3 cut(s) 74, 461, 478
LweI GCATC 6 cut(s) 88, 304, 455, 477, 673, 695
MaeI CTAG 2 cut(s) 593, 764
MaeII ACGT 1 cut(s) 777
MaeIII GTNAC 4 cut(s) 124, 196, 655, 865
MalI GATC 2 cut(s) 363, 530
MboI GATC 2 cut(s) 361, 528
MhlI GDGCHC 2 cut(s) 169, 668
MluCI AATT 3 cut(s) 136, 336, 429
Mly113I GGCGCC 1 cut(s) 831
MmeI TCCRAC 2 cut(s) 246, 602
Mph1103I ATGCAT 3 cut(s) 82, 470, 688
MreI CGCCGGCG 1 cut(s) 833
MroNI GCCGGC 1 cut(s) 833
MseI TTAA 1 cut(s) 729
MslI CAYNNNNRTG 1 cut(s) 696
MspA1I CMGCKG 1 cut(s) 87
MspI CCGG 3 cut(s) 178, 378, 834
MspR9I CCNGG 3 cut(s) 178, 179, 669
Mva1269I GAATGC 1 cut(s) 80
MvaI CCWGG 1 cut(s) 669
MwoI GCNNNNNNNGC 6 cut(s) 173, 455, 614, 794, 984, 998
NaeI GCCGGC 1 cut(s) 835
NarI GGCGCC 1 cut(s) 831
NciI CCSGG 2 cut(s) 178, 179
NcoI CCATGG 1 cut(s) 826
NdeI CATATG 1 cut(s) 454
NdeII GATC 2 cut(s) 361, 528
NgoMIV GCCGGC 1 cut(s) 833
NlaIII CATG 7 cut(s) 232, 335, 371, 803, 830, 917, 982
NlaIV GGNNCC 1 cut(s) 832
NmuCI GTSAC 1 cut(s) 124
NsiI ATGCAT 3 cut(s) 82, 470, 688
NspI RCATGY 2 cut(s) 803, 982
PaeI GCATGC 1 cut(s) 982
PctI GAATGC 1 cut(s) 80
PdiI GCCGGC 1 cut(s) 835
PfeI GAWTC 1 cut(s) 910
PkrI GCNGC 4 cut(s) 89, 451, 493, 790
PluTI GGCGCC 1 cut(s) 834
Psp6I CCWGG 1 cut(s) 667
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 1 cut(s) 832
PspPI GGNCC 4 cut(s) 175, 190, 549, 823
PstI CTGCAG 2 cut(s) 92, 569
PvuII CAGCTG 1 cut(s) 87
RsaI GTAC 1 cut(s) 972
RsaNI GTAC 1 cut(s) 971
RseI CAYNNNNRTG 1 cut(s) 696
SaqAI TTAA 1 cut(s) 729
SatI GCNGC 4 cut(s) 88, 450, 492, 789
Sau3AI GATC 2 cut(s) 361, 528
Sau96I GGNCC 4 cut(s) 175, 190, 549, 823
ScrFI CCNGG 3 cut(s) 178, 179, 669
SduI GDGCHC 2 cut(s) 169, 668
SfaNI GCATC 6 cut(s) 88, 304, 455, 477, 673, 695
SfcI CTRYAG 2 cut(s) 88, 565
SfoI GGCGCC 1 cut(s) 832
SgrAI CRCCGGYG 1 cut(s) 833
SinI GGWCC 3 cut(s) 190, 549, 823
SmaI CCCGGG 1 cut(s) 179
SmiMI CAYNNNNRTG 1 cut(s) 696
SmlI CTYRAG 1 cut(s) 938
SmoI CTYRAG 1 cut(s) 938
SphI GCATGC 1 cut(s) 982
Sse9I AATT 3 cut(s) 136, 336, 429
SsiI CCGC 2 cut(s) 788, 837
SspDI GGCGCC 1 cut(s) 830
SspMI CTAG 2 cut(s) 593, 764
StyD4I CCNGG 3 cut(s) 176, 177, 667
StyI CCWWGG 3 cut(s) 592, 633, 826
TaaI ACNGT 2 cut(s) 661, 758
TaiI ACGT 1 cut(s) 780
TaqI TCGA 1 cut(s) 534
TasI AATT 3 cut(s) 136, 336, 429
TatI WGTACW 1 cut(s) 970
TauI GCSGC 1 cut(s) 791
TfiI GAWTC 1 cut(s) 910
Tru1I TTAA 1 cut(s) 729
Tru9I TTAA 1 cut(s) 729
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 3 cut(s) 87, 449, 491
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 6 cut(s) 245, 531, 656, 828, 902, 978
TspMI CCCGGG 1 cut(s) 177
TspRI CASTG 1 cut(s) 136
VpaK11BI GGWCC 3 cut(s) 190, 549, 823
XapI RAATTY 1 cut(s) 136
XceI RCATGY 2 cut(s) 803, 982
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XmaI CCCGGG 1 cut(s) 177
XmaJI CCTAGG 1 cut(s) 592
XspI CTAG 2 cut(s) 593, 764
Zsp2I ATGCAT 3 cut(s) 82, 470, 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.