Rh2DG592000

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0016903
Physical Location & Seq
Reverse (-)
82443313 .. 82445126
1814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG592000.1

Sequence Viewer

Length: 1032 bp
ATGGAAGAAGTGGTGGTGCCAAAGCCATTGATAATCTCTTTAGAGGAGGAACACAAAAACTGCGTAAACTACGCATTCAGGTGCGGTTGGGATATTTCAAGAGAGAAGAATCTGGTTTGTGTGACCAGTGGGAATTCTTACCTGGGTTCTCACATAGTTAGGGCTCTTTTGGCTCGCGGATATCGGATTCGAGTCACCATTCAAAACCAAGAGGATTATGAAGACATGAAGAAGCTAATGAGAGATGAAGAGATTAACCAACTAGAAAGTGTTGTTGTGGCAAAGATGGGAAACTTGGATAGGCTTTGTGATGCTTTTAGGGGCTGCCATGCAATTTTTCATACTTCTTCCTTCATTGATCCACATGGTGTCTCTGGTTATTCTGGACAAATGGCATTTCTTGAAACTGAAGGGGCAAAGAATGTGATCGAAGCTTGTGGCAGGGCAGCGCATGCAAAGAGATGCATCTTCACTTCCTCTCTTCTTGCTTCTATTTGGACAGGTGATAGTGAAAACAAGATCATTGATGAGTTTAGCTGGAGCAATGAAGAATTCTGCAGGGAAAACAAGCTTTGGCTGGCCTTAGGGAAGACCACTGCAGAGATGGCTTGTTGGAGAAAGTCAAAGGAAATGAAAGTCAATCTTGTGACAGTTTGCCCTGGCCTTCTCATGGACTCATATGCTCACAAGGACAGCTCTATACCCTATCTTAAAGGTGGCAAGGACATGCTCAAAAATGGTCACCTAGCCATTGAGGATGTGAAGAAGGCTGCAGAGGCGCACGTCTATGTATATGAGGCCATGGATAGTGGAGCATGTGGGAGATACCTGTGCTTTGGGAAGGTTGTAAGAACAAGAAGTGAAGCCCTTGAACTAGAAAACATGTTAAGGGTTCAGGGTTTGTTATCAGGAGGAAGCCTTGAAGTCTTTGATGAAGAAGCAGGTGCAGAAATAAACAGCATGCTTAGTAATGCAAAGCTGGTCAGATTGATTCAAGCTTCTCAAAGATTAACGTGCAAGCCAGTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD NAD_binding_10

Protein Analysis

343

Amino Acids

38.32

Weight (kDa)

6.18

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 39 - 220 2.4e-08 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 41 - 189 1.2e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 47 - 165 1.7e-07 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016903)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45521
malus_domestica MD09G1082100.v1.1
prunus_persica Prupe.3G241700_v2.0.a1
pyrus_communis pycom09g00860
rosa_chinensis RchiOBHm_Chr2g0163471
rosa_laevigata RLG00000021404
rosa_multiflora Rmu_co8460151.1_g000001
rosa_roxburghii Rroxscaffold_2G00087290
rosa_rugosa Rorug02G0503000
rosa_samantha Rh2AG570000 Rh2BG582200 Rh2CG552000 Rh2DG592000
rosa_wichuraiana Rw2G047190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 932
Acc36I ACCTGC 1 cut(s) 932
AccB1I GGYRCC 1 cut(s) 16
AccII CGCG 1 cut(s) 177
AciI CCGC 2 cut(s) 84, 177
AclWI GGATC 1 cut(s) 353
AcsI RAATTY 2 cut(s) 133, 551
AcuI CTGAAG 1 cut(s) 429
AdeI CACNNNGTG 1 cut(s) 368
AfiI CCNNNNNNNGG 1 cut(s) 670
AflIII ACRYGT 1 cut(s) 882
AgsI TTSAA 6 cut(s) 99, 203, 404, 872, 923, 995
AjiI CACGTC 1 cut(s) 784
AjnI CCWGG 2 cut(s) 141, 658
AluBI AGCT 7 cut(s) 235, 434, 537, 571, 696, 979, 998
AluI AGCT 7 cut(s) 235, 434, 537, 571, 696, 979, 998
Alw26I GTCTC 1 cut(s) 376
AlwI GGATC 1 cut(s) 353
AoxI GGCC 3 cut(s) 579, 661, 798
ApeKI GCWGC 3 cut(s) 324, 446, 770
ApoI RAATTY 2 cut(s) 133, 551
AspLEI GCGC 2 cut(s) 451, 781
AsuHPI GGTGA 3 cut(s) 187, 515, 734
AxyI CCTNAGG 1 cut(s) 583
BanI GGYRCC 1 cut(s) 16
BanII GRGCYC 1 cut(s) 166
BbsI GAAGAC 2 cut(s) 228, 596
BbvI GCAGC 3 cut(s) 311, 458, 757
BccI CCATC 2 cut(s) 280, 598
BciT130I CCWGG 2 cut(s) 143, 660
BcoDI GTCTC 1 cut(s) 376
BfaI CTAG 3 cut(s) 263, 746, 875
BfmI CTRYAG 3 cut(s) 556, 597, 771
BfuAI ACCTGC 1 cut(s) 932
BisI GCNGC 3 cut(s) 325, 447, 771
BlsI GCNGC 3 cut(s) 326, 448, 772
Bme1390I CCNGG 2 cut(s) 143, 660
BmgBI CACGTC 1 cut(s) 784
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 2 cut(s) 143, 660
BmsI GCATC 3 cut(s) 301, 452, 474
BpiI GAAGAC 2 cut(s) 228, 596
BpmI CTGGAG 1 cut(s) 559
BsaJI CCNNGG 3 cut(s) 142, 658, 801
Bsc4I CCNNNNNNNGG 1 cut(s) 670
Bse1I ACTGG 2 cut(s) 126, 1022
Bse21I CCTNAGG 1 cut(s) 583
Bse3DI GCAATG 1 cut(s) 550
BseBI CCWGG 2 cut(s) 143, 660
BseDI CCNNGG 3 cut(s) 142, 658, 801
BseGI GGATG 1 cut(s) 763
BseLI CCNNNNNNNGG 1 cut(s) 670
BseMI GCAATG 1 cut(s) 550
BseNI ACTGG 2 cut(s) 126, 1022
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 3 cut(s) 311, 458, 757
BsgI GTGCAG 1 cut(s) 966
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 3 cut(s) 581, 663, 800
BshNI GGYRCC 1 cut(s) 16
BslI CCNNNNNNNGG 1 cut(s) 670
BsmAI GTCTC 1 cut(s) 376
BsmI GAATGC 1 cut(s) 74
BsnI GGCC 3 cut(s) 581, 663, 800
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 3 cut(s) 358, 426, 519
Bsp19I CCATGG 1 cut(s) 801
BspACI CCGC 2 cut(s) 84, 177
BspANI GGCC 3 cut(s) 581, 663, 800
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 1 cut(s) 18
BspMAI CTGCAG 3 cut(s) 560, 601, 775
BspMI ACCTGC 1 cut(s) 932
BspPI GGATC 1 cut(s) 353
BspT107I GGYRCC 1 cut(s) 16
BsrDI GCAATG 1 cut(s) 550
BsrI ACTGG 2 cut(s) 126, 1022
BssECI CCNNGG 3 cut(s) 142, 658, 801
BssMI GATC 3 cut(s) 358, 426, 519
BssT1I CCWWGG 1 cut(s) 801
Bst2UI CCWGG 2 cut(s) 143, 660
Bst4CI ACNGT 1 cut(s) 652
Bst6I CTCTTC 2 cut(s) 243, 486
BstAPI GCANNNNNTGC 1 cut(s) 452
BstC8I GCNNGC 5 cut(s) 175, 453, 579, 962, 1019
BstDEI CTNAG 2 cut(s) 583, 965
BstDSI CCRYGG 1 cut(s) 801
BstEII GGTNACC 1 cut(s) 740
BstF5I GGATG 1 cut(s) 763
BstFNI CGCG 1 cut(s) 177
BstHHI GCGC 2 cut(s) 451, 781
BstKTI GATC 3 cut(s) 361, 429, 522
BstMAI GTCTC 1 cut(s) 376
BstMBI GATC 3 cut(s) 358, 426, 519
BstMWI GCNNNNNNNGC 4 cut(s) 170, 452, 605, 776
BstNI CCWGG 2 cut(s) 143, 660
BstNSI RCATGY 5 cut(s) 455, 730, 819, 886, 964
BstPI GGTNACC 1 cut(s) 740
BstSCI CCNGG 2 cut(s) 141, 658
BstSFI CTRYAG 3 cut(s) 556, 597, 771
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 3 cut(s) 311, 458, 757
BstV2I GAAGAC 2 cut(s) 228, 596
Bsu36I CCTNAGG 1 cut(s) 583
BsuRI GGCC 3 cut(s) 581, 663, 800
BtgI CCRYGG 1 cut(s) 801
BtrI CACGTC 1 cut(s) 784
BtsCI GGATG 1 cut(s) 763
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 2 cut(s) 133, 594
BveI ACCTGC 1 cut(s) 932
Cac8I GCNNGC 5 cut(s) 175, 453, 579, 962, 1019
CfoI GCGC 2 cut(s) 451, 781
DdeI CTNAG 2 cut(s) 583, 965
DpnI GATC 3 cut(s) 360, 428, 521
DpnII GATC 3 cut(s) 358, 426, 519
DraIII CACNNNGTG 1 cut(s) 368
Eam1104I CTCTTC 2 cut(s) 243, 486
EarI CTCTTC 2 cut(s) 243, 486
Eco130I CCWWGG 1 cut(s) 801
Eco24I GRGCYC 1 cut(s) 166
Eco32I GATATC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 429
Eco81I CCTNAGG 1 cut(s) 583
Eco91I GGTNACC 1 cut(s) 740
EcoO65I GGTNACC 1 cut(s) 740
EcoRI GAATTC 2 cut(s) 133, 551
EcoRII CCWGG 2 cut(s) 141, 658
EcoRV GATATC 1 cut(s) 182
EcoT14I CCWWGG 1 cut(s) 801
EcoT22I ATGCAT 1 cut(s) 467
EcoT38I GRGCYC 1 cut(s) 166
ErhI CCWWGG 1 cut(s) 801
FalI AAGNNNNNCTT 2 cut(s) 627, 659
FauNDI CATATG 1 cut(s) 679
Fnu4HI GCNGC 3 cut(s) 325, 447, 771
FokI GGATG 1 cut(s) 770
FriOI GRGCYC 1 cut(s) 166
Fsp4HI GCNGC 3 cut(s) 325, 447, 771
FspBI CTAG 3 cut(s) 263, 746, 875
GlaI GCGC 2 cut(s) 450, 780
GluI GCNGC 3 cut(s) 325, 447, 771
GsuI CTGGAG 1 cut(s) 559
HaeIII GGCC 3 cut(s) 581, 663, 800
HhaI GCGC 2 cut(s) 451, 781
Hin6I GCGC 2 cut(s) 449, 779
HinP1I GCGC 2 cut(s) 449, 779
HindIII AAGCTT 3 cut(s) 432, 569, 996
HinfI GANTC 5 cut(s) 109, 187, 192, 674, 991
HphI GGTGA 3 cut(s) 187, 515, 734
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 2 cut(s) 186, 986
Hpy188III TCNNGA 4 cut(s) 99, 384, 401, 909
Hpy8I GTNNAC 1 cut(s) 67
HpyAV CCTTC 5 cut(s) 361, 404, 674, 760, 835
HpyCH4III ACNGT 1 cut(s) 652
HpyCH4IV ACGT 2 cut(s) 783, 1013
HpyCH4V TGCA 9 cut(s) 332, 455, 465, 558, 599, 773, 947, 974, 1017
HpyF10VI GCNNNNNNNGC 4 cut(s) 170, 452, 605, 776
HpyF3I CTNAG 2 cut(s) 583, 965
HpySE526I ACGT 2 cut(s) 783, 1013
HspAI GCGC 2 cut(s) 449, 779
Kzo9I GATC 3 cut(s) 358, 426, 519
LmnI GCTCC 2 cut(s) 540, 812
Lsp1109I GCAGC 3 cut(s) 311, 458, 757
LweI GCATC 3 cut(s) 301, 452, 474
MaeI CTAG 3 cut(s) 263, 746, 875
MaeII ACGT 2 cut(s) 783, 1013
MaeIII GTNAC 4 cut(s) 121, 193, 646, 740
MalI GATC 3 cut(s) 360, 428, 521
MboI GATC 3 cut(s) 358, 426, 519
MhlI GDGCHC 1 cut(s) 166
MluCI AATT 3 cut(s) 133, 333, 551
MlyI GAGTC 2 cut(s) 201, 668
MmeI TCCRAC 1 cut(s) 593
MnlI CCTC 8 cut(s) 37, 40, 205, 487, 748, 769, 790, 905
Mph1103I ATGCAT 1 cut(s) 467
MseI TTAA 4 cut(s) 255, 711, 887, 1010
MslI CAYNNNNRTG 2 cut(s) 79, 786
MspR9I CCNGG 2 cut(s) 143, 660
Mva1269I GAATGC 1 cut(s) 74
MvaI CCWGG 2 cut(s) 143, 660
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 4 cut(s) 170, 452, 605, 776
NcoI CCATGG 1 cut(s) 801
NdeI CATATG 1 cut(s) 679
NdeII GATC 3 cut(s) 358, 426, 519
NlaIV GGNNCC 1 cut(s) 18
NmuCI GTSAC 4 cut(s) 121, 193, 646, 740
NsiI ATGCAT 1 cut(s) 467
NspI RCATGY 5 cut(s) 455, 730, 819, 886, 964
PaeI GCATGC 2 cut(s) 455, 964
PaqCI CACCTGC 1 cut(s) 932
PciI ACATGT 1 cut(s) 882
PctI GAATGC 1 cut(s) 74
PfeI GAWTC 3 cut(s) 109, 187, 991
PkrI GCNGC 3 cut(s) 326, 448, 772
PleI GAGTC 2 cut(s) 200, 668
PpsI GAGTC 2 cut(s) 200, 668
PscI ACATGT 1 cut(s) 882
Psp6I CCWGG 2 cut(s) 141, 658
PspEI GGTNACC 1 cut(s) 740
PspGI CCWGG 2 cut(s) 141, 658
PspN4I GGNNCC 1 cut(s) 18
PstI CTGCAG 3 cut(s) 560, 601, 775
RseI CAYNNNNRTG 2 cut(s) 79, 786
SaqAI TTAA 4 cut(s) 255, 711, 887, 1010
SatI GCNGC 3 cut(s) 325, 447, 771
Sau3AI GATC 3 cut(s) 358, 426, 519
SchI GAGTC 2 cut(s) 201, 668
ScrFI CCNGG 2 cut(s) 143, 660
SduI GDGCHC 1 cut(s) 166
SfaNI GCATC 3 cut(s) 301, 452, 474
SfcI CTRYAG 3 cut(s) 556, 597, 771
SmiMI CAYNNNNRTG 2 cut(s) 79, 786
SphI GCATGC 2 cut(s) 455, 964
Sse9I AATT 3 cut(s) 133, 333, 551
SsiI CCGC 2 cut(s) 84, 177
SspMI CTAG 3 cut(s) 263, 746, 875
StyD4I CCNGG 2 cut(s) 141, 658
StyI CCWWGG 1 cut(s) 801
TaaI ACNGT 1 cut(s) 652
TaiI ACGT 2 cut(s) 786, 1016
TaqI TCGA 2 cut(s) 190, 429
TasI AATT 3 cut(s) 133, 333, 551
TfiI GAWTC 3 cut(s) 109, 187, 991
Tru1I TTAA 4 cut(s) 255, 711, 887, 1010
Tru9I TTAA 4 cut(s) 255, 711, 887, 1010
TscAI CASTG 2 cut(s) 133, 601
TseFI GTSAC 4 cut(s) 121, 193, 646, 740
TseI GCWGC 3 cut(s) 324, 446, 770
Tsp45I GTSAC 4 cut(s) 121, 193, 646, 740
TspDTI ATGAA 8 cut(s) 234, 242, 261, 329, 343, 561, 647, 948
TspRI CASTG 2 cut(s) 133, 601
XapI RAATTY 2 cut(s) 133, 551
XceI RCATGY 5 cut(s) 455, 730, 819, 886, 964
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XspI CTAG 3 cut(s) 263, 746, 875
Zsp2I ATGCAT 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.