Rw2G047190

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016903
Physical Location & Seq
Reverse (-)
74565350 .. 74567166
1817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G047190.1

Sequence Viewer

Length: 1038 bp
ATGGCAATGGAAGAAGTGGTGGTGCCAAAGCCATTGATGATCTCTTTAGAGGAGGAACACAAAAACTGCGTAAACTACGCATTCAGGTGCAGTTGGGATATTTCAACAGAGAAGAATCTGGTTTGTGTGACCAGTGGGAATTCTTGCCTGGGTTCTCACATAGTTAGGGCTCTTTTGGCTCGCGGATATCGGATTCGAGTCACCATTCAAAACCAAGAGGATTATGAAGACATGAAGAAGCTAATGAGAGATGAAGAGATAAACCAACTAGAAAGTGTTGTTGTGGCAAAGATGGGAAACTTGGATAGGCTTTGTGATGCTTTTAGGGGCTGCCACGCAATTTTTCATACTTCTTCCTTCATTGATCCACATGGTGTCTCTGGTTATTCTGGACAAATGGCATTTCTTGAAACTGAAGGGGCAAAGAATGTGATCGAAGCTTGTGGCAGGGCAGCGCATGCAAAGAGATGCATCTTCACTTCCTCTCTTCTTGCTTCTATTTGGACAGGTGATAGTGAAAACAAGATCATTGATGAGTTTAGCTGGAGCAATGAAGAATTCTGCAGGGAAAACAAGCTTTGGCTGGCCTTGGGGAAGACCACTGCAGAGATGGCTTGTTGGAGAAAGTCAAAGGAAATGAAAGTCAATCTTGTGACAGTTTGCCCTGGCCTTCTCATGGACTCATATGCTCACAAGGACAGCTCTATACCCTATCTTAAAGGTGGCAAGGACATGCTCAAAAATGGTCACCTAGCCATTGAGGATGTGAAGAAGGCTGCAGAGGCGCACGTCTATGTATATGAGGCAATGGATAGTGGAGCATGTGGGAGATACCTGTGCTTTGGGAAGGTTGTAAGAACAAGAAGTGAAGCCCTTGAACTAGAAAACATGTTAAGGGTTCAGGGTTTGTTATCAGGAGGAAGCCTTGAAGTCTTTGATGAAGAAGCAGGTGCAGAAATAAACAGCATGCTTAGTAATGCAAAGCTGGTCAGATTGATTCAAGCTTCTCAAAGATTATCGTGCAAGCCATTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD NAD_binding_10

Protein Analysis

345

Amino Acids

38.46

Weight (kDa)

6.04

Isoelectric Point (pI)

38.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 41 - 222 7.1e-08 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 43 - 191 7.3e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 50 - 167 2.4e-07 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016903)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45521
malus_domestica MD09G1082100.v1.1
prunus_persica Prupe.3G241700_v2.0.a1
pyrus_communis pycom09g00860
rosa_chinensis RchiOBHm_Chr2g0163471
rosa_laevigata RLG00000021404
rosa_multiflora Rmu_co8460151.1_g000001
rosa_roxburghii Rroxscaffold_2G00087290
rosa_rugosa Rorug02G0503000
rosa_samantha Rh2AG570000 Rh2BG582200 Rh2CG552000 Rh2DG592000
rosa_wichuraiana Rw2G047190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 938
Acc36I ACCTGC 1 cut(s) 938
AccB1I GGYRCC 1 cut(s) 22
AccII CGCG 1 cut(s) 183
AciI CCGC 1 cut(s) 183
AclWI GGATC 1 cut(s) 359
AcsI RAATTY 2 cut(s) 139, 557
AcuI CTGAAG 1 cut(s) 435
AdeI CACNNNGTG 1 cut(s) 374
AfiI CCNNNNNNNGG 1 cut(s) 676
AflIII ACRYGT 1 cut(s) 888
AgsI TTSAA 6 cut(s) 105, 209, 410, 878, 929, 1001
AjiI CACGTC 1 cut(s) 790
AjnI CCWGG 2 cut(s) 147, 664
AluBI AGCT 7 cut(s) 241, 440, 543, 577, 702, 985, 1004
AluI AGCT 7 cut(s) 241, 440, 543, 577, 702, 985, 1004
Alw26I GTCTC 1 cut(s) 382
AlwI GGATC 1 cut(s) 359
AoxI GGCC 2 cut(s) 585, 667
ApeKI GCWGC 3 cut(s) 330, 452, 776
ApoI RAATTY 2 cut(s) 139, 557
AspLEI GCGC 2 cut(s) 457, 787
AsuHPI GGTGA 3 cut(s) 193, 521, 740
BanI GGYRCC 1 cut(s) 22
BanII GRGCYC 1 cut(s) 172
BbsI GAAGAC 2 cut(s) 234, 602
BbvI GCAGC 3 cut(s) 317, 464, 763
BccI CCATC 2 cut(s) 286, 604
BciT130I CCWGG 2 cut(s) 149, 666
BcoDI GTCTC 1 cut(s) 382
BfaI CTAG 3 cut(s) 269, 752, 881
BfmI CTRYAG 3 cut(s) 562, 603, 777
BfuAI ACCTGC 1 cut(s) 938
BisI GCNGC 3 cut(s) 331, 453, 777
BlsI GCNGC 3 cut(s) 332, 454, 778
Bme1390I CCNGG 2 cut(s) 149, 666
BmgBI CACGTC 1 cut(s) 790
BmiI GGNNCC 1 cut(s) 24
BmrFI CCNGG 2 cut(s) 149, 666
BmsI GCATC 3 cut(s) 307, 458, 480
BpiI GAAGAC 2 cut(s) 234, 602
BpmI CTGGAG 1 cut(s) 565
BsaJI CCNNGG 3 cut(s) 148, 588, 664
Bsc4I CCNNNNNNNGG 1 cut(s) 676
Bse1I ACTGG 1 cut(s) 132
Bse3DI GCAATG 3 cut(s) 12, 556, 813
BseBI CCWGG 2 cut(s) 149, 666
BseDI CCNNGG 3 cut(s) 148, 588, 664
BseGI GGATG 1 cut(s) 769
BseLI CCNNNNNNNGG 1 cut(s) 676
BseMI GCAATG 3 cut(s) 12, 556, 813
BseNI ACTGG 1 cut(s) 132
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 3 cut(s) 317, 464, 763
BsgI GTGCAG 2 cut(s) 109, 972
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 2 cut(s) 587, 669
BshNI GGYRCC 1 cut(s) 22
BslI CCNNNNNNNGG 1 cut(s) 676
BsmAI GTCTC 1 cut(s) 382
BsmI GAATGC 1 cut(s) 80
BsnI GGCC 2 cut(s) 587, 669
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 4 cut(s) 39, 364, 432, 525
BspACI CCGC 1 cut(s) 183
BspANI GGCC 2 cut(s) 587, 669
BspFNI CGCG 1 cut(s) 183
BspLI GGNNCC 1 cut(s) 24
BspMAI CTGCAG 3 cut(s) 566, 607, 781
BspMI ACCTGC 1 cut(s) 938
BspPI GGATC 1 cut(s) 359
BspT107I GGYRCC 1 cut(s) 22
BsrDI GCAATG 3 cut(s) 12, 556, 813
BsrI ACTGG 1 cut(s) 132
BssECI CCNNGG 3 cut(s) 148, 588, 664
BssMI GATC 4 cut(s) 39, 364, 432, 525
BssT1I CCWWGG 1 cut(s) 588
Bst2UI CCWGG 2 cut(s) 149, 666
Bst4CI ACNGT 1 cut(s) 658
Bst6I CTCTTC 2 cut(s) 249, 492
BstAPI GCANNNNNTGC 1 cut(s) 458
BstC8I GCNNGC 5 cut(s) 181, 459, 585, 968, 1025
BstDEI CTNAG 1 cut(s) 971
BstEII GGTNACC 1 cut(s) 746
BstF5I GGATG 1 cut(s) 769
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 2 cut(s) 457, 787
BstKTI GATC 4 cut(s) 42, 367, 435, 528
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 4 cut(s) 39, 364, 432, 525
BstMWI GCNNNNNNNGC 4 cut(s) 176, 458, 611, 782
BstNI CCWGG 2 cut(s) 149, 666
BstNSI RCATGY 5 cut(s) 461, 736, 825, 892, 970
BstPI GGTNACC 1 cut(s) 746
BstSCI CCNGG 2 cut(s) 147, 664
BstSFI CTRYAG 3 cut(s) 562, 603, 777
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 3 cut(s) 317, 464, 763
BstV2I GAAGAC 2 cut(s) 234, 602
BsuRI GGCC 2 cut(s) 587, 669
BtrI CACGTC 1 cut(s) 790
BtsCI GGATG 1 cut(s) 769
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 2 cut(s) 139, 600
BveI ACCTGC 1 cut(s) 938
Cac8I GCNNGC 5 cut(s) 181, 459, 585, 968, 1025
CfoI GCGC 2 cut(s) 457, 787
CviAII CATG 8 cut(s) 232, 371, 458, 676, 733, 822, 889, 967
DdeI CTNAG 1 cut(s) 971
DpnI GATC 4 cut(s) 41, 366, 434, 527
DpnII GATC 4 cut(s) 39, 364, 432, 525
DraIII CACNNNGTG 1 cut(s) 374
Eam1104I CTCTTC 2 cut(s) 249, 492
EarI CTCTTC 2 cut(s) 249, 492
Eco130I CCWWGG 1 cut(s) 588
Eco24I GRGCYC 1 cut(s) 172
Eco32I GATATC 1 cut(s) 188
Eco57I CTGAAG 1 cut(s) 435
Eco91I GGTNACC 1 cut(s) 746
EcoO65I GGTNACC 1 cut(s) 746
EcoRI GAATTC 2 cut(s) 139, 557
EcoRII CCWGG 2 cut(s) 147, 664
EcoRV GATATC 1 cut(s) 188
EcoT14I CCWWGG 1 cut(s) 588
EcoT22I ATGCAT 1 cut(s) 473
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 588
FaeI CATG 8 cut(s) 235, 374, 461, 679, 736, 825, 892, 970
FalI AAGNNNNNCTT 2 cut(s) 633, 665
FatI CATG 8 cut(s) 231, 370, 457, 675, 732, 821, 888, 966
FauNDI CATATG 1 cut(s) 685
Fnu4HI GCNGC 3 cut(s) 331, 453, 777
FokI GGATG 1 cut(s) 776
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 3 cut(s) 331, 453, 777
FspBI CTAG 3 cut(s) 269, 752, 881
GlaI GCGC 2 cut(s) 456, 786
GluI GCNGC 3 cut(s) 331, 453, 777
GsuI CTGGAG 1 cut(s) 565
HaeIII GGCC 2 cut(s) 587, 669
HhaI GCGC 2 cut(s) 457, 787
Hin1II CATG 8 cut(s) 235, 374, 461, 679, 736, 825, 892, 970
Hin6I GCGC 2 cut(s) 455, 785
HinP1I GCGC 2 cut(s) 455, 785
HindIII AAGCTT 3 cut(s) 438, 575, 1002
HinfI GANTC 5 cut(s) 115, 193, 198, 680, 997
HphI GGTGA 3 cut(s) 193, 521, 740
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 2 cut(s) 192, 992
Hpy188III TCNNGA 3 cut(s) 390, 407, 915
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 5 cut(s) 367, 410, 680, 766, 841
HpyCH4III ACNGT 1 cut(s) 658
HpyCH4IV ACGT 1 cut(s) 789
HpyCH4V TGCA 9 cut(s) 90, 461, 471, 564, 605, 779, 953, 980, 1023
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 458, 611, 782
HpyF3I CTNAG 1 cut(s) 971
HpySE526I ACGT 1 cut(s) 789
Hsp92II CATG 8 cut(s) 235, 374, 461, 679, 736, 825, 892, 970
HspAI GCGC 2 cut(s) 455, 785
Kzo9I GATC 4 cut(s) 39, 364, 432, 525
LmnI GCTCC 2 cut(s) 546, 818
Lsp1109I GCAGC 3 cut(s) 317, 464, 763
LweI GCATC 3 cut(s) 307, 458, 480
MaeI CTAG 3 cut(s) 269, 752, 881
MaeII ACGT 1 cut(s) 789
MaeIII GTNAC 4 cut(s) 127, 199, 652, 746
MalI GATC 4 cut(s) 41, 366, 434, 527
MboI GATC 4 cut(s) 39, 364, 432, 525
MhlI GDGCHC 1 cut(s) 172
MluCI AATT 3 cut(s) 139, 339, 557
MlyI GAGTC 2 cut(s) 207, 674
MmeI TCCRAC 1 cut(s) 599
MnlI CCTC 8 cut(s) 43, 46, 211, 493, 754, 775, 796, 911
Mph1103I ATGCAT 1 cut(s) 473
MseI TTAA 2 cut(s) 717, 893
MslI CAYNNNNRTG 2 cut(s) 85, 792
MspR9I CCNGG 2 cut(s) 149, 666
Mva1269I GAATGC 1 cut(s) 80
MvaI CCWGG 2 cut(s) 149, 666
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 4 cut(s) 176, 458, 611, 782
NdeI CATATG 1 cut(s) 685
NdeII GATC 4 cut(s) 39, 364, 432, 525
NlaIII CATG 8 cut(s) 235, 374, 461, 679, 736, 825, 892, 970
NlaIV GGNNCC 1 cut(s) 24
NmuCI GTSAC 4 cut(s) 127, 199, 652, 746
NsiI ATGCAT 1 cut(s) 473
NspI RCATGY 5 cut(s) 461, 736, 825, 892, 970
PaeI GCATGC 2 cut(s) 461, 970
PaqCI CACCTGC 1 cut(s) 938
PciI ACATGT 1 cut(s) 888
PctI GAATGC 1 cut(s) 80
PfeI GAWTC 3 cut(s) 115, 193, 997
PkrI GCNGC 3 cut(s) 332, 454, 778
PleI GAGTC 2 cut(s) 206, 674
PpsI GAGTC 2 cut(s) 206, 674
PscI ACATGT 1 cut(s) 888
Psp6I CCWGG 2 cut(s) 147, 664
PspEI GGTNACC 1 cut(s) 746
PspGI CCWGG 2 cut(s) 147, 664
PspN4I GGNNCC 1 cut(s) 24
PstI CTGCAG 3 cut(s) 566, 607, 781
RseI CAYNNNNRTG 2 cut(s) 85, 792
SaqAI TTAA 2 cut(s) 717, 893
SatI GCNGC 3 cut(s) 331, 453, 777
Sau3AI GATC 4 cut(s) 39, 364, 432, 525
SchI GAGTC 2 cut(s) 207, 674
ScrFI CCNGG 2 cut(s) 149, 666
SduI GDGCHC 1 cut(s) 172
SfaNI GCATC 3 cut(s) 307, 458, 480
SfcI CTRYAG 3 cut(s) 562, 603, 777
SmiMI CAYNNNNRTG 2 cut(s) 85, 792
SphI GCATGC 2 cut(s) 461, 970
Sse9I AATT 3 cut(s) 139, 339, 557
SsiI CCGC 1 cut(s) 183
SspMI CTAG 3 cut(s) 269, 752, 881
StyD4I CCNGG 2 cut(s) 147, 664
StyI CCWWGG 1 cut(s) 588
TaaI ACNGT 1 cut(s) 658
TaiI ACGT 1 cut(s) 792
TaqI TCGA 2 cut(s) 196, 435
TasI AATT 3 cut(s) 139, 339, 557
TfiI GAWTC 3 cut(s) 115, 193, 997
Tru1I TTAA 2 cut(s) 717, 893
Tru9I TTAA 2 cut(s) 717, 893
TscAI CASTG 2 cut(s) 139, 607
TseFI GTSAC 4 cut(s) 127, 199, 652, 746
TseI GCWGC 3 cut(s) 330, 452, 776
Tsp45I GTSAC 4 cut(s) 127, 199, 652, 746
TspDTI ATGAA 8 cut(s) 240, 248, 267, 335, 349, 567, 653, 954
TspRI CASTG 2 cut(s) 139, 607
XapI RAATTY 2 cut(s) 139, 557
XceI RCATGY 5 cut(s) 461, 736, 825, 892, 970
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XspI CTAG 3 cut(s) 269, 752, 881
Zsp2I ATGCAT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.