RLG00000021404

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016903
Physical Location & Seq
Reverse (-)
74884230 .. 74886063
1834 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021404

Sequence Viewer

Length: 1038 bp
ATGGCAATGGAAGAAGTGGTGGTGCCGAAGCCATTGATGATCTCTTTAGAGGAGGAACACAAAAACTGCGTAAACTATGCATTCAGGTGCAGTTGGGATATTTCAAGAGAGAAGAACCTGGTTTGTGTGACCAGTGGGAATTCTTACCTGGGTTCTCACATAGTTAGGGCTCTTTTGGCTCGCGGATATCTGGTTCGAGTCACCATTCAAAACCAAGAGGATTATGAAGACATGAAGAAGCTAATGAGAGATGAAGAGATAAACCAACTAGAAAGTGTTGTTGTGGCAAAGATGGGAAACTTGGATAGGCTTTGTGATGCTTTTAGGGGCTGCCATGCAATTTTTCATACTTCCTCCTTCATTGATCCGCATGGTGTCTCTGGTTATTCTGGACAAATGGCATTTCTTGAAACTGAAGGGGCAATGAATGTGATCGAAGCTTGTGGCAGGGCAGCGTATGCAAAGAGATGCATCTTCACCTCCTCTCTCCTTGCTTCTATTTGGACAGGTGATAGTGAAAACAAGATCATTGATGAGTTTAGCTGGAGCAATGAAGAATTCTGCAGGGAAAACAAGCTTTGGCTGGCTTTGGGGAAGACCACTGCGGAGATGGCTTGTTGGAGAAAGTCAAAGGAAATGAAAGTCAATCTTGTGACAGTTTGCCCTGGCCTTCTCATGGACTCATGTGCTCACAAGGACAGCTCTATACCCTATCTTAAAGGTGGCAAGGACATGCTCAAAAATGGTCACCTAGCCATTGAGGATGTGAAGAAGGCTGCAGAGGCGCACGTCTATGTATATGAGGCCATGGATAGTGGAGCATGTGGGAGATACCTGTGCTTTGGGAAGGTTGTAAGAACAAGAAGTGAAGCCCTTGAACTAGAAAACATGTTAAGGGTTCAGGGTTTGTTATCCGGAGGAAGCCTTGAAGTCTTTGATGAAGAAGCAGGTGCAGAAATAAACAGCATGCTTAGTAATGCAAAGCTGGTCAGATTGATTCAAGCTTCTCAAAGATTATCGTGCAAGCCAGTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD NAD_binding_10

Protein Analysis

346

Amino Acids

38.47

Weight (kDa)

5.84

Isoelectric Point (pI)

39.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 41 - 221 1.7e-07 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 43 - 189 5.5e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 49 - 167 2.8e-06 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016903)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45521
malus_domestica MD09G1082100.v1.1
prunus_persica Prupe.3G241700_v2.0.a1
pyrus_communis pycom09g00860
rosa_chinensis RchiOBHm_Chr2g0163471
rosa_laevigata RLG00000021404
rosa_multiflora Rmu_co8460151.1_g000001
rosa_roxburghii Rroxscaffold_2G00087290
rosa_rugosa Rorug02G0503000
rosa_samantha Rh2AG570000 Rh2BG582200 Rh2CG552000 Rh2DG592000
rosa_wichuraiana Rw2G047190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 938
Acc36I ACCTGC 1 cut(s) 938
AccB1I GGYRCC 1 cut(s) 22
AccII CGCG 1 cut(s) 183
AccIII TCCGGA 1 cut(s) 914
AciI CCGC 3 cut(s) 183, 368, 605
AclWI GGATC 1 cut(s) 359
AcsI RAATTY 2 cut(s) 139, 557
AcuI CTGAAG 1 cut(s) 435
AfiI CCNNNNNNNGG 1 cut(s) 676
AflIII ACRYGT 1 cut(s) 888
AgsI TTSAA 6 cut(s) 105, 209, 410, 878, 929, 1001
AjiI CACGTC 1 cut(s) 790
AjnI CCWGG 3 cut(s) 117, 147, 664
AloI GAACNNNNNNTCC 2 cut(s) 177, 209
AluBI AGCT 7 cut(s) 241, 440, 543, 577, 702, 985, 1004
AluI AGCT 7 cut(s) 241, 440, 543, 577, 702, 985, 1004
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 1 cut(s) 382
AlwI GGATC 1 cut(s) 359
Aor13HI TCCGGA 1 cut(s) 914
AoxI GGCC 2 cut(s) 667, 804
ApeKI GCWGC 3 cut(s) 330, 452, 776
ApoI RAATTY 2 cut(s) 139, 557
AspLEI GCGC 1 cut(s) 787
AsuHPI GGTGA 4 cut(s) 193, 469, 521, 740
BanI GGYRCC 1 cut(s) 22
BanII GRGCYC 1 cut(s) 172
BbsI GAAGAC 2 cut(s) 234, 602
Bbv12I GWGCWC 1 cut(s) 691
BbvI GCAGC 3 cut(s) 317, 464, 763
BccI CCATC 2 cut(s) 286, 604
BciT130I CCWGG 3 cut(s) 119, 149, 666
BcoDI GTCTC 1 cut(s) 382
BfaI CTAG 3 cut(s) 269, 752, 881
BfmI CTRYAG 2 cut(s) 562, 777
BfuAI ACCTGC 1 cut(s) 938
BisI GCNGC 3 cut(s) 331, 453, 777
BlsI GCNGC 3 cut(s) 332, 454, 778
Bme1390I CCNGG 3 cut(s) 119, 149, 666
BmgBI CACGTC 1 cut(s) 790
BmiI GGNNCC 1 cut(s) 24
BmrFI CCNGG 3 cut(s) 119, 149, 666
BmsI GCATC 3 cut(s) 307, 458, 480
BpiI GAAGAC 2 cut(s) 234, 602
BpmI CTGGAG 1 cut(s) 565
BsaJI CCNNGG 3 cut(s) 148, 664, 807
BsaWI WCCGGW 1 cut(s) 914
Bsc4I CCNNNNNNNGG 1 cut(s) 676
Bse1I ACTGG 2 cut(s) 132, 1028
Bse3DI GCAATG 3 cut(s) 12, 429, 556
BseAI TCCGGA 1 cut(s) 914
BseBI CCWGG 3 cut(s) 119, 149, 666
BseDI CCNNGG 3 cut(s) 148, 664, 807
BseGI GGATG 1 cut(s) 769
BseLI CCNNNNNNNGG 1 cut(s) 676
BseMI GCAATG 3 cut(s) 12, 429, 556
BseNI ACTGG 2 cut(s) 132, 1028
BseRI GAGGAG 2 cut(s) 65, 472
BseXI GCAGC 3 cut(s) 317, 464, 763
BsgI GTGCAG 2 cut(s) 109, 972
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 2 cut(s) 669, 806
BshNI GGYRCC 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 691
BsiSI CCGG 1 cut(s) 915
BslI CCNNNNNNNGG 1 cut(s) 676
BsmAI GTCTC 1 cut(s) 382
BsmI GAATGC 1 cut(s) 80
BsnI GGCC 2 cut(s) 669, 806
Bsp1286I GDGCHC 2 cut(s) 172, 691
Bsp13I TCCGGA 1 cut(s) 914
Bsp143I GATC 4 cut(s) 39, 364, 432, 525
Bsp19I CCATGG 1 cut(s) 807
BspACI CCGC 3 cut(s) 183, 368, 605
BspANI GGCC 2 cut(s) 669, 806
BspEI TCCGGA 1 cut(s) 914
BspFNI CGCG 1 cut(s) 183
BspLI GGNNCC 1 cut(s) 24
BspMAI CTGCAG 2 cut(s) 566, 781
BspMI ACCTGC 1 cut(s) 938
BspPI GGATC 1 cut(s) 359
BspT107I GGYRCC 1 cut(s) 22
BsrDI GCAATG 3 cut(s) 12, 429, 556
BsrI ACTGG 2 cut(s) 132, 1028
BssECI CCNNGG 3 cut(s) 148, 664, 807
BssMI GATC 4 cut(s) 39, 364, 432, 525
BssT1I CCWWGG 1 cut(s) 807
Bst2UI CCWGG 3 cut(s) 119, 149, 666
Bst4CI ACNGT 1 cut(s) 658
Bst6I CTCTTC 1 cut(s) 249
BstAPI GCANNNNNTGC 1 cut(s) 458
BstC8I GCNNGC 4 cut(s) 181, 585, 968, 1025
BstDEI CTNAG 1 cut(s) 971
BstDSI CCRYGG 1 cut(s) 807
BstEII GGTNACC 1 cut(s) 746
BstF5I GGATG 1 cut(s) 769
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 1 cut(s) 787
BstKTI GATC 4 cut(s) 42, 367, 435, 528
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 4 cut(s) 39, 364, 432, 525
BstMWI GCNNNNNNNGC 4 cut(s) 176, 458, 611, 782
BstNI CCWGG 3 cut(s) 119, 149, 666
BstNSI RCATGY 4 cut(s) 736, 825, 892, 970
BstPI GGTNACC 1 cut(s) 746
BstSCI CCNGG 3 cut(s) 117, 147, 664
BstSFI CTRYAG 2 cut(s) 562, 777
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 3 cut(s) 317, 464, 763
BstV2I GAAGAC 2 cut(s) 234, 602
BsuRI GGCC 2 cut(s) 669, 806
BtgI CCRYGG 1 cut(s) 807
BtrI CACGTC 1 cut(s) 790
BtsCI GGATG 1 cut(s) 769
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 2 cut(s) 139, 600
BveI ACCTGC 1 cut(s) 938
Cac8I GCNNGC 4 cut(s) 181, 585, 968, 1025
CfoI GCGC 1 cut(s) 787
CsiI ACCWGGT 1 cut(s) 117
DdeI CTNAG 1 cut(s) 971
DpnI GATC 4 cut(s) 41, 366, 434, 527
DpnII GATC 4 cut(s) 39, 364, 432, 525
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
Eco130I CCWWGG 1 cut(s) 807
Eco24I GRGCYC 1 cut(s) 172
Eco32I GATATC 1 cut(s) 188
Eco57I CTGAAG 1 cut(s) 435
Eco91I GGTNACC 1 cut(s) 746
EcoO65I GGTNACC 1 cut(s) 746
EcoRI GAATTC 2 cut(s) 139, 557
EcoRII CCWGG 3 cut(s) 117, 147, 664
EcoRV GATATC 1 cut(s) 188
EcoT14I CCWWGG 1 cut(s) 807
EcoT22I ATGCAT 2 cut(s) 82, 473
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 807
FalI AAGNNNNNCTT 2 cut(s) 633, 665
Fnu4HI GCNGC 3 cut(s) 331, 453, 777
FokI GGATG 1 cut(s) 776
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 3 cut(s) 331, 453, 777
FspBI CTAG 3 cut(s) 269, 752, 881
GlaI GCGC 1 cut(s) 786
GluI GCNGC 3 cut(s) 331, 453, 777
GsuI CTGGAG 1 cut(s) 565
HaeIII GGCC 2 cut(s) 669, 806
HapII CCGG 1 cut(s) 915
HhaI GCGC 1 cut(s) 787
Hin6I GCGC 1 cut(s) 785
HinP1I GCGC 1 cut(s) 785
HindIII AAGCTT 3 cut(s) 438, 575, 1002
HinfI GANTC 3 cut(s) 198, 680, 997
HpaII CCGG 1 cut(s) 915
HphI GGTGA 4 cut(s) 193, 469, 521, 740
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 1 cut(s) 992
Hpy188III TCNNGA 4 cut(s) 105, 390, 407, 915
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 5 cut(s) 367, 410, 680, 766, 841
HpyCH4III ACNGT 1 cut(s) 658
HpyCH4IV ACGT 1 cut(s) 789
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 458, 611, 782
HpyF3I CTNAG 1 cut(s) 971
HpySE526I ACGT 1 cut(s) 789
HspAI GCGC 1 cut(s) 785
Kpn2I TCCGGA 1 cut(s) 914
Kzo9I GATC 4 cut(s) 39, 364, 432, 525
LmnI GCTCC 2 cut(s) 546, 818
Lsp1109I GCAGC 3 cut(s) 317, 464, 763
LweI GCATC 3 cut(s) 307, 458, 480
MabI ACCWGGT 1 cut(s) 117
MaeI CTAG 3 cut(s) 269, 752, 881
MaeII ACGT 1 cut(s) 789
MaeIII GTNAC 4 cut(s) 127, 199, 652, 746
MalI GATC 4 cut(s) 41, 366, 434, 527
MboI GATC 4 cut(s) 39, 364, 432, 525
MhlI GDGCHC 2 cut(s) 172, 691
MluCI AATT 3 cut(s) 139, 339, 557
MlyI GAGTC 2 cut(s) 207, 674
MmeI TCCRAC 1 cut(s) 599
Mph1103I ATGCAT 2 cut(s) 82, 473
MroI TCCGGA 1 cut(s) 914
MseI TTAA 2 cut(s) 717, 893
MslI CAYNNNNRTG 2 cut(s) 85, 792
MspI CCGG 1 cut(s) 915
MspR9I CCNGG 3 cut(s) 119, 149, 666
Mva1269I GAATGC 1 cut(s) 80
MvaI CCWGG 3 cut(s) 119, 149, 666
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 4 cut(s) 176, 458, 611, 782
NcoI CCATGG 1 cut(s) 807
NdeII GATC 4 cut(s) 39, 364, 432, 525
NlaIV GGNNCC 1 cut(s) 24
NmuCI GTSAC 4 cut(s) 127, 199, 652, 746
NsiI ATGCAT 2 cut(s) 82, 473
NspI RCATGY 4 cut(s) 736, 825, 892, 970
PaeI GCATGC 1 cut(s) 970
PaqCI CACCTGC 1 cut(s) 938
PciI ACATGT 1 cut(s) 888
PctI GAATGC 1 cut(s) 80
PfeI GAWTC 1 cut(s) 997
PkrI GCNGC 3 cut(s) 332, 454, 778
PleI GAGTC 2 cut(s) 206, 674
PpsI GAGTC 2 cut(s) 206, 674
PscI ACATGT 1 cut(s) 888
Psp6I CCWGG 3 cut(s) 117, 147, 664
PspEI GGTNACC 1 cut(s) 746
PspGI CCWGG 3 cut(s) 117, 147, 664
PspN4I GGNNCC 1 cut(s) 24
PstI CTGCAG 2 cut(s) 566, 781
RseI CAYNNNNRTG 2 cut(s) 85, 792
SaqAI TTAA 2 cut(s) 717, 893
SatI GCNGC 3 cut(s) 331, 453, 777
Sau3AI GATC 4 cut(s) 39, 364, 432, 525
SchI GAGTC 2 cut(s) 207, 674
ScrFI CCNGG 3 cut(s) 119, 149, 666
SduI GDGCHC 2 cut(s) 172, 691
SexAI ACCWGGT 1 cut(s) 117
SfaNI GCATC 3 cut(s) 307, 458, 480
SfcI CTRYAG 2 cut(s) 562, 777
SmiMI CAYNNNNRTG 2 cut(s) 85, 792
SphI GCATGC 1 cut(s) 970
Sse9I AATT 3 cut(s) 139, 339, 557
SsiI CCGC 3 cut(s) 183, 368, 605
SspMI CTAG 3 cut(s) 269, 752, 881
StyD4I CCNGG 3 cut(s) 117, 147, 664
StyI CCWWGG 1 cut(s) 807
TaaI ACNGT 1 cut(s) 658
TaiI ACGT 1 cut(s) 792
TaqI TCGA 2 cut(s) 196, 435
TasI AATT 3 cut(s) 139, 339, 557
TfiI GAWTC 1 cut(s) 997
Tru1I TTAA 2 cut(s) 717, 893
Tru9I TTAA 2 cut(s) 717, 893
TscAI CASTG 2 cut(s) 139, 607
TseFI GTSAC 4 cut(s) 127, 199, 652, 746
TseI GCWGC 3 cut(s) 330, 452, 776
Tsp45I GTSAC 4 cut(s) 127, 199, 652, 746
TspDTI ATGAA 9 cut(s) 240, 248, 267, 335, 349, 440, 567, 653, 954
TspRI CASTG 2 cut(s) 139, 607
XapI RAATTY 2 cut(s) 139, 557
XceI RCATGY 4 cut(s) 736, 825, 892, 970
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XspI CTAG 3 cut(s) 269, 752, 881
Zsp2I ATGCAT 2 cut(s) 82, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.