Rroxscaffold_2G00087290

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016903
Physical Location & Seq
Forward (+)
9374463 .. 9376882
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00087290.1

Sequence Viewer

Length: 948 bp
ATGGCAATGGAAGAAGTGGTGGTGCCAAAGCCATTGATGATCTCTTCAGAGGAGGAACACAAAAACTGCGTAAACTACGCATTCAGGTGCAGTTGGGATATTTCAAGAGAGAAGAATCTGGTTTGTGTGACCAGTGGGAATTCTTACCTGGGTTCTCACATAGTTAGGGCTCTTTTGGCTCGCGGATATCGGGTTCGAGTCACCATTCAAAACCAAGAGGATTATGAAGACATGAAGAAGCTAATGAGAGATGAAGAGATAAACCAACTAGAAAGTGTTGTTGTGGCAAAGATGGGAAACTTGGATAGGCTTTGTGATGCTTTTAGGGGCTGCCATGCAATTTTTCATACTTCCTCCTTCATTGATCCACATGGTGTCTCTGGTTATTCTGGACAAATGGCATTTCTTGAAACTGAAGGGGCAAAGAATGTGATCGAAGCTTGTGGCAGGGCAGCGTATGCAAAGAGATGCATCTTCACTTCCTCTCTTCTTGCTTCTATTTGGACAGGTGATAGTGAAAACAAGATCATTGATGAGTTTAGCTGGAGCAATGAAGAATTCTGCAGGGAAAACAAGCTTTGGCTGGCTTTGGGGAAGACCACTGCAGAGATGGCTTGTGGCAAGGACATGCTCAAAAATGGTCACCTAGCCATTGAGGATGTGCAGAAGGCCGCAGAGGCGCACGTCTGTGTATATGAGGCCATGGATAATGGAGCATGTGGAAGATACCTGTGCTTTGGGAAGGTTGTAAGAACAAGAAGTGAAGCCCTTGAACTAGAAAACATGTTAAGGGTTCAGGGTTTGTTATCAGGAGGAAGCCTTGAAGTCTTTGATGAAGAAACAGGTGCAGAAATAAACAGCATGCTTAGTAATGCAAAGCTGGTCAGATTGATTCAAGCTTCTCAGAGATTATTGTGCAAGCGGCAAGCCAGTAGTTTAGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD NAD_binding_10

Protein Analysis

315

Amino Acids

35.14

Weight (kDa)

5.52

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 41 - 173 3.1e-07 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 43 - 165 2.3e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 49 - 166 2.8e-07 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016903)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g45521
malus_domestica MD09G1082100.v1.1
prunus_persica Prupe.3G241700_v2.0.a1
pyrus_communis pycom09g00860
rosa_chinensis RchiOBHm_Chr2g0163471
rosa_laevigata RLG00000021404
rosa_multiflora Rmu_co8460151.1_g000001
rosa_roxburghii Rroxscaffold_2G00087290
rosa_rugosa Rorug02G0503000
rosa_samantha Rh2AG570000 Rh2BG582200 Rh2CG552000 Rh2DG592000
rosa_wichuraiana Rw2G047190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 22
AccII CGCG 1 cut(s) 183
AciI CCGC 3 cut(s) 183, 672, 922
AclWI GGATC 1 cut(s) 359
AcsI RAATTY 2 cut(s) 139, 557
AcuI CTGAAG 2 cut(s) 30, 435
AdeI CACNNNGTG 1 cut(s) 374
AflIII ACRYGT 1 cut(s) 783
AgsI TTSAA 6 cut(s) 105, 209, 410, 773, 824, 896
AjiI CACGTC 1 cut(s) 685
AjnI CCWGG 1 cut(s) 147
AleI CACNNNNGTG 1 cut(s) 687
AloI GAACNNNNNNTCC 2 cut(s) 177, 209
AluBI AGCT 6 cut(s) 241, 440, 543, 577, 880, 899
AluI AGCT 6 cut(s) 241, 440, 543, 577, 880, 899
Alw26I GTCTC 1 cut(s) 382
AlwI GGATC 1 cut(s) 359
AoxI GGCC 2 cut(s) 669, 699
ApeKI GCWGC 2 cut(s) 330, 452
ApoI RAATTY 2 cut(s) 139, 557
AspLEI GCGC 1 cut(s) 682
AsuHPI GGTGA 3 cut(s) 193, 521, 635
BanI GGYRCC 1 cut(s) 22
BanII GRGCYC 1 cut(s) 172
BbsI GAAGAC 2 cut(s) 234, 602
BbvI GCAGC 2 cut(s) 317, 464
BccI CCATC 2 cut(s) 286, 604
BciT130I CCWGG 1 cut(s) 149
BcoDI GTCTC 1 cut(s) 382
BfaI CTAG 3 cut(s) 269, 647, 776
BfmI CTRYAG 2 cut(s) 562, 603
BglI GCCNNNNNGGC 1 cut(s) 677
BisI GCNGC 4 cut(s) 331, 453, 672, 923
BlsI GCNGC 4 cut(s) 332, 454, 673, 924
Bme1390I CCNGG 1 cut(s) 149
BmgBI CACGTC 1 cut(s) 685
BmiI GGNNCC 1 cut(s) 24
BmrFI CCNGG 1 cut(s) 149
BmsI GCATC 3 cut(s) 307, 458, 480
BpiI GAAGAC 2 cut(s) 234, 602
BpmI CTGGAG 1 cut(s) 565
BsaJI CCNNGG 2 cut(s) 148, 702
Bse1I ACTGG 2 cut(s) 132, 930
Bse3DI GCAATG 2 cut(s) 12, 556
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 2 cut(s) 148, 702
BseGI GGATG 1 cut(s) 664
BseMI GCAATG 2 cut(s) 12, 556
BseMII CTCAG 1 cut(s) 917
BseNI ACTGG 2 cut(s) 132, 930
BseRI GAGGAG 1 cut(s) 65
BseXI GCAGC 2 cut(s) 317, 464
BsgI GTGCAG 3 cut(s) 109, 683, 867
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 2 cut(s) 671, 701
BshNI GGYRCC 1 cut(s) 22
BsmAI GTCTC 1 cut(s) 382
BsmI GAATGC 1 cut(s) 80
BsnI GGCC 2 cut(s) 671, 701
Bsp1286I GDGCHC 1 cut(s) 172
Bsp143I GATC 4 cut(s) 39, 364, 432, 525
Bsp19I CCATGG 1 cut(s) 702
BspACI CCGC 3 cut(s) 183, 672, 922
BspANI GGCC 2 cut(s) 671, 701
BspCNI CTCAG 1 cut(s) 916
BspFNI CGCG 1 cut(s) 183
BspHI TCATGA 1 cut(s) 944
BspLI GGNNCC 1 cut(s) 24
BspMAI CTGCAG 2 cut(s) 566, 607
BspPI GGATC 1 cut(s) 359
BspT107I GGYRCC 1 cut(s) 22
BsrDI GCAATG 2 cut(s) 12, 556
BsrI ACTGG 2 cut(s) 132, 930
BssECI CCNNGG 2 cut(s) 148, 702
BssMI GATC 4 cut(s) 39, 364, 432, 525
BssT1I CCWWGG 1 cut(s) 702
Bst2UI CCWGG 1 cut(s) 149
Bst6I CTCTTC 3 cut(s) 49, 249, 492
BstAPI GCANNNNNTGC 1 cut(s) 458
BstC8I GCNNGC 5 cut(s) 181, 585, 863, 920, 927
BstDEI CTNAG 2 cut(s) 866, 903
BstDSI CCRYGG 1 cut(s) 702
BstEII GGTNACC 1 cut(s) 641
BstF5I GGATG 1 cut(s) 664
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 1 cut(s) 682
BstKTI GATC 4 cut(s) 42, 367, 435, 528
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 4 cut(s) 39, 364, 432, 525
BstMWI GCNNNNNNNGC 4 cut(s) 176, 458, 611, 677
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 4 cut(s) 631, 720, 787, 865
BstPI GGTNACC 1 cut(s) 641
BstSCI CCNGG 1 cut(s) 147
BstSFI CTRYAG 2 cut(s) 562, 603
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 317, 464
BstV2I GAAGAC 2 cut(s) 234, 602
BsuRI GGCC 2 cut(s) 671, 701
BtgI CCRYGG 1 cut(s) 702
BtrI CACGTC 1 cut(s) 685
BtsCI GGATG 1 cut(s) 664
BtsI GCAGTG 1 cut(s) 600
BtsIMutI CAGTG 2 cut(s) 139, 600
Cac8I GCNNGC 5 cut(s) 181, 585, 863, 920, 927
CciI TCATGA 1 cut(s) 944
CfoI GCGC 1 cut(s) 682
CviAII CATG 9 cut(s) 232, 335, 371, 628, 703, 717, 784, 862, 945
DdeI CTNAG 2 cut(s) 866, 903
DpnI GATC 4 cut(s) 41, 366, 434, 527
DpnII GATC 4 cut(s) 39, 364, 432, 525
DraIII CACNNNGTG 1 cut(s) 374
Eam1104I CTCTTC 3 cut(s) 49, 249, 492
EarI CTCTTC 3 cut(s) 49, 249, 492
Eco130I CCWWGG 1 cut(s) 702
Eco24I GRGCYC 1 cut(s) 172
Eco32I GATATC 1 cut(s) 188
Eco57I CTGAAG 2 cut(s) 30, 435
Eco91I GGTNACC 1 cut(s) 641
EcoO65I GGTNACC 1 cut(s) 641
EcoRI GAATTC 2 cut(s) 139, 557
EcoRII CCWGG 1 cut(s) 147
EcoRV GATATC 1 cut(s) 188
EcoT14I CCWWGG 1 cut(s) 702
EcoT22I ATGCAT 1 cut(s) 473
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 702
FaeI CATG 9 cut(s) 235, 338, 374, 631, 706, 720, 787, 865, 948
FatI CATG 9 cut(s) 231, 334, 370, 627, 702, 716, 783, 861, 944
Fnu4HI GCNGC 4 cut(s) 331, 453, 672, 923
FokI GGATG 1 cut(s) 671
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 4 cut(s) 331, 453, 672, 923
FspBI CTAG 3 cut(s) 269, 647, 776
GlaI GCGC 1 cut(s) 681
GluI GCNGC 4 cut(s) 331, 453, 672, 923
GsuI CTGGAG 1 cut(s) 565
HaeIII GGCC 2 cut(s) 671, 701
HhaI GCGC 1 cut(s) 682
Hin1II CATG 9 cut(s) 235, 338, 374, 631, 706, 720, 787, 865, 948
Hin6I GCGC 1 cut(s) 680
HinP1I GCGC 1 cut(s) 680
HindIII AAGCTT 3 cut(s) 438, 575, 897
HinfI GANTC 4 cut(s) 115, 198, 892, 941
HphI GGTGA 3 cut(s) 193, 521, 635
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 3 cut(s) 49, 887, 906
Hpy188III TCNNGA 5 cut(s) 105, 390, 407, 810, 945
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 4 cut(s) 367, 410, 661, 736
HpyCH4IV ACGT 1 cut(s) 684
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 458, 611, 677
HpyF3I CTNAG 2 cut(s) 866, 903
HpySE526I ACGT 1 cut(s) 684
Hsp92II CATG 9 cut(s) 235, 338, 374, 631, 706, 720, 787, 865, 948
HspAI GCGC 1 cut(s) 680
Kzo9I GATC 4 cut(s) 39, 364, 432, 525
LmnI GCTCC 2 cut(s) 546, 713
Lsp1109I GCAGC 2 cut(s) 317, 464
LweI GCATC 3 cut(s) 307, 458, 480
MaeI CTAG 3 cut(s) 269, 647, 776
MaeII ACGT 1 cut(s) 684
MaeIII GTNAC 3 cut(s) 127, 199, 641
MalI GATC 4 cut(s) 41, 366, 434, 527
MboI GATC 4 cut(s) 39, 364, 432, 525
MhlI GDGCHC 1 cut(s) 172
MluCI AATT 3 cut(s) 139, 339, 557
MlyI GAGTC 1 cut(s) 207
MnlI CCTC 9 cut(s) 43, 46, 211, 364, 493, 649, 670, 691, 806
Mph1103I ATGCAT 1 cut(s) 473
MseI TTAA 1 cut(s) 788
MslI CAYNNNNRTG 2 cut(s) 85, 687
MspR9I CCNGG 1 cut(s) 149
Mva1269I GAATGC 1 cut(s) 80
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 4 cut(s) 176, 458, 611, 677
NcoI CCATGG 1 cut(s) 702
NdeII GATC 4 cut(s) 39, 364, 432, 525
NlaIII CATG 9 cut(s) 235, 338, 374, 631, 706, 720, 787, 865, 948
NlaIV GGNNCC 1 cut(s) 24
NmuCI GTSAC 3 cut(s) 127, 199, 641
NsiI ATGCAT 1 cut(s) 473
NspI RCATGY 4 cut(s) 631, 720, 787, 865
OliI CACNNNNGTG 1 cut(s) 687
PaeI GCATGC 1 cut(s) 865
PagI TCATGA 1 cut(s) 944
PciI ACATGT 1 cut(s) 783
PctI GAATGC 1 cut(s) 80
PfeI GAWTC 3 cut(s) 115, 892, 941
PkrI GCNGC 4 cut(s) 332, 454, 673, 924
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
PscI ACATGT 1 cut(s) 783
Psp6I CCWGG 1 cut(s) 147
PspEI GGTNACC 1 cut(s) 641
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 1 cut(s) 24
PstI CTGCAG 2 cut(s) 566, 607
RseI CAYNNNNRTG 2 cut(s) 85, 687
SaqAI TTAA 1 cut(s) 788
SatI GCNGC 4 cut(s) 331, 453, 672, 923
Sau3AI GATC 4 cut(s) 39, 364, 432, 525
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 1 cut(s) 149
SduI GDGCHC 1 cut(s) 172
SfaNI GCATC 3 cut(s) 307, 458, 480
SfcI CTRYAG 2 cut(s) 562, 603
SmiMI CAYNNNNRTG 2 cut(s) 85, 687
SphI GCATGC 1 cut(s) 865
Sse9I AATT 3 cut(s) 139, 339, 557
SsiI CCGC 3 cut(s) 183, 672, 922
SspMI CTAG 3 cut(s) 269, 647, 776
StyD4I CCNGG 1 cut(s) 147
StyI CCWWGG 1 cut(s) 702
TaiI ACGT 1 cut(s) 687
TaqI TCGA 2 cut(s) 196, 435
TasI AATT 3 cut(s) 139, 339, 557
TauI GCSGC 2 cut(s) 674, 925
TfiI GAWTC 3 cut(s) 115, 892, 941
Tru1I TTAA 1 cut(s) 788
Tru9I TTAA 1 cut(s) 788
TscAI CASTG 2 cut(s) 139, 607
TseFI GTSAC 3 cut(s) 127, 199, 641
TseI GCWGC 2 cut(s) 330, 452
Tsp45I GTSAC 3 cut(s) 127, 199, 641
TspDTI ATGAA 8 cut(s) 240, 248, 267, 335, 349, 567, 849, 933
TspRI CASTG 2 cut(s) 139, 607
XapI RAATTY 2 cut(s) 139, 557
XceI RCATGY 4 cut(s) 631, 720, 787, 865
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XspI CTAG 3 cut(s) 269, 647, 776
Zsp2I ATGCAT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.