Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008562

Physical Location & Seq

Genomic Coordinates Forward (+)

6706862 .. 6712417

Gene Structure 5556 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1091500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2403 bp

ATGGGAAGGAAGAGATCTGTCCTGGAGCACAAGAGCTTGAAGATCTATGCTGAGGTTTCAGTGGGGTTGCTTTTAATCTACTTTGCTCAAGTTTCTCTTGGAGCAACTAGTTCTAGTGATGTTGCTGCAATTAACAACTTACACGCTGCGCTTGGCACGCCTGTGCTTCCCGGGTGGACTTCTGCTGGAGGAGACCCGTGTGGTGATGCATGGCAAGGTGTTCAATGCAACGATTCTGTAATATTGGGAATAGTTCTTAATGGCGCTAATTTGGGAGGAGAACTGGGTGAAAACTTGGGAATGTTTGCTTCAATACGGGCTATAGATCTAAGCAACAATCATATCGGAGGAAGTATTCCATCCAATTTACCTGCCACCCTGCGCAGCTTTTTTCTTTCAGCTAACCAATTTACCGGAAGCATCCCAGATACTGTAGCCTCGTTAACTGTGCTCACAGCAATGTCTCTTAATAATAATCAATTAACCGGAGAAATACCAGATTCCTTCCAAGGCATTCCAGGGTTGACCAACTTAGATTTTTCCGGTAACAATTTGAGCGGCCAACTGCCACCCTCTCTAAGAAGCCTAATAACCCTGACCACTCTACATTTGCAGAACAATCTGCTGTCTGGAACCCTCGACGTTTTGCAGGATCTTCCCCTAAGTGACTTGAATATAGAGAACAACCTTTTTGCTGGACCCATACCTGAAAAGTTGCTCAGCATCCCGAATTTCAAAAAAGATGGGAATCCATTCAATTCTTCTCTTGCTCCAGTCGCTTCACCAACATCCCCGTTGACTCCACCCACATCCACGTCGAGCCCGCCAGCACCTCCACTGCCATTTTGGGGACCACCTACTGGGAAAACACCTGGCAGAAGTCCTCCAAAAAATGGGACGGTTGATGGACCATCTTCACAAGATACATCAAATTCTGGAAAGCAGAAGAAATTTCTAACTAAGAAAAGGGTGGTTGGAATATCAATTGGAGGGGCTTTAGTGTTTATTATTTTTATACTAGCGATTCTGTTTTTTATGCCAAGACGTAGTAGACGAGGAGAAGAGGCTGACGGAATTTCCAAGCAGCATCAAATAGGTGCATATACAGGTGACAGGGAAAACCCTCCCAGGGACAATGGGTCCTTGGTGGTTCTACCGGCCAATCCAATACAGAAAGTTCCAAAAGAAGCAGTTGTGACGCCCAAAGAGGATAATGAACCAGAAACCAGAAGAACATGGGCAATTCCAAAGCCAAATGACGAGCAAGATAGAAAGGTACATAAAATTGCTGCAGCACCAAAGCGAGAGGATAGTTTGAGTGCACTTGATTTGTATTCAATGCCTGCTCCTCCTCCTCCTCCACGTCCCCCTCCTCCACCACCGCCTGTTCCGAAGGTCATTGTGAAGCCAATTGTGCCTACGGAACTGCCTACACAGAAGCCTGCCACCAAAAGTCCAAACCGTACAATTTCTGCAAAGCGGTTTACCATTGCTTCCCTTCAGCAACATACAAACAGCTTTTCTCCGGATAACCTTCTAGGATCAGGCATGCTGGGAAGTGTCTATAGGGCACAGCTACCTAGCGGAAAGCTACTTGCAGTCAAGAAATTGGACAAAAGAGCTTCTAGTCAGCAGAAAGAGGACGAGTTTCTTGACCTGGTAAACAGCATTGACAGAATCCGGCATGCTAATGTTGTCGAGCTTATGGGTTACTGTGCAGAGCATGGTCAAAGGCTTCTTATCTACGAGTATTGCAGTAATGGTTCACTGCATGATGCTCTGCACATAGATGATGAATTCAAGAAGAAACTCCCATGGAATGCTCGTATTAGAATGGCACTTGAAGCTGCAAGAGCCTTAGAGTATTTACATGAAGTCTGCCAGCCACCTATTGTACACAGAAATTTCAAGGCTGCCAACCTTCTCCTTGATGATGACCTTTCTGTGCGTGTTTCTGACTGTGGCTTGGCTCCCTTGATATCATCGGGATCTGTGAGTCAGTTGTCAGGACAGCTTCTATCGGCCTATGGTTATGGAGCTCCAGAATTTGAGTCAGGAATTTATACTCATCAGAGTGATGTTTACAGCTTTGGGGTGGTAATGTTGGAACTATTAACTGGCCGGAAGTCCCATGACAGCACAAGGAACCGAGGGGAGCAATTCCTGGTTAGATGGGCCACTACCCAGCTTCATGACATTGATGCACTATCGAGGATGGTTGATCCTTCTCTAAATGGACAATATCCTACAAAGTCTTTATCGCATTTTGCAGACATTATATCTCGATGCATTCAGTGGGAGCCAGAGTTCAGGCCACCAATGTCTGGAGTTGTCCAAGACCTTTTAGACATGATACGGCGAGAGCCTAATGGCAGTGGATCAAGTGAAGACTCACTCTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007623 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030054 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048511 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_8 Pkinase_Tyr

Protein Analysis

801

Amino Acids

86.53

Weight (kDa)

7.0

Isoelectric Point (pI)

42.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	126 - 185	6.2e-08	Leucine rich repeat
Pkinase	PF00069	510 - 779	3.9e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	511 - 781	3.6e-43	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008562)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03390 AT4G03390
fragaria_vesca	FvH4_6g44950 FvH4_6g44950 FvH4_6g44950
malus_domestica	MD09G1091500.v1.1 MD17G1081400.v1.1
prunus_persica	Prupe.3G233500_v2.0.a1 Prupe.3G233500_v2.0.a1 Prupe.3G233500_v2.0.a1 Prupe.3G233500_v2.0.a1 Prupe.3G233500_v2.0.a1 Prupe.3G233500_v2.0.a1
pyrus_communis	pycom09g01720 pycom17g07970
rosa_chinensis	RchiOBHm_Chr2g0162181
rosa_laevigata	RLG00000021326
rosa_multiflora	Rmu_sc0004020.1_g000030
rosa_roxburghii	Rroxscaffold_2G00088260
rosa_rugosa	Rorug02G0495000 Rorug02G0495100
rosa_samantha	Rh2BG574600
rosa_wichuraiana	Rw2G046500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	383
Acc36I	ACCTGC	1 cut(s)	379
AccB7I	CCANNNNNTGG	3 cut(s)	860, 893, 2324
AccBSI	CCGCTC	1 cut(s)	558
AccI	GTMKAC	1 cut(s)	1051
AccIII	TCCGGA	1 cut(s)	1525
AciI	CCGC	5 cut(s)	558, 824, 1382, 1480, 1584
AclWI	GGATC	5 cut(s)	660, 1549, 1996, 2216, 2386
AcoI	YGGCCR	3 cut(s)	559, 1158, 2119
AcsI	RAATTY	8 cut(s)	730, 931, 950, 1074, 1796, 1903, 2045, 2058
AcuI	CTGAAG	1 cut(s)	1484
AcyI	GRCGYC	1 cut(s)	1199
AfaI	GTAC	3 cut(s)	1278, 1465, 1896
AfiI	CCNNNNNNNGG	5 cut(s)	848, 860, 893, 1129, 2324
AhdI	GACNNNNNGTC	1 cut(s)	1138
AhlI	ACTAGT	1 cut(s)	107
AjiI	CACGTC	2 cut(s)	816, 1364
AjnI	CCWGG	6 cut(s)	21, 517, 871, 1127, 1656, 2163
AleI	CACNNNNGTG	1 cut(s)	161
AloI	GAACNNNNNNTCC	2 cut(s)	2291, 2323
Alw21I	GWGCWC	4 cut(s)	30, 453, 1324, 2041
Alw26I	GTCTC	2 cut(s)	186, 468
Alw44I	GTGCAC	1 cut(s)	1320
AlwI	GGATC	5 cut(s)	660, 1549, 1996, 2216, 2386
AlwNI	CAGNNNCTG	1 cut(s)	431
Ama87I	CYCGRG	1 cut(s)	170
Aor13HI	TCCGGA	1 cut(s)	1525
AoxI	GGCC	6 cut(s)	559, 1158, 2022, 2119, 2175, 2312
ApaLI	GTGCAC	1 cut(s)	1320
ApeKI	GCWGC	8 cut(s)	125, 146, 384, 1084, 1289, 1292, 1847, 1913
ApoI	RAATTY	8 cut(s)	730, 931, 950, 1074, 1796, 1903, 2045, 2058
Asp700I	GAANNNNTTC	1 cut(s)	752
AspLEI	GCGC	3 cut(s)	151, 266, 384
AspS9I	GGNCC	5 cut(s)	698, 851, 908, 1140, 2175
AsuC2I	CCSGG	2 cut(s)	171, 172
AsuHPI	GGTGA	4 cut(s)	215, 299, 774, 1121
AvaI	CYCGRG	1 cut(s)	170
AvaII	GGWCC	4 cut(s)	698, 851, 908, 1140
BaeGI	GKGCMC	2 cut(s)	1324, 1573
BanII	GRGCYC	2 cut(s)	824, 2041
BbsI	GAAGAC	1 cut(s)	2394
Bbv12I	GWGCWC	4 cut(s)	30, 453, 1324, 2041
BbvCI	CCTCAGC	1 cut(s)	51
BbvI	GCAGC	8 cut(s)	112, 133, 396, 1096, 1276, 1304, 1834, 1900
BccI	CCATC	6 cut(s)	367, 737, 899, 919, 2166, 2209
BceAI	ACGGC	1 cut(s)	2372
BciT130I	CCWGG	6 cut(s)	23, 519, 873, 1129, 1658, 2165
BcnI	CCSGG	2 cut(s)	171, 172
BcoDI	GTCTC	2 cut(s)	186, 468
BcuI	ACTAGT	1 cut(s)	107
BfaI	CTAG	6 cut(s)	108, 114, 1019, 1538, 1581, 1626
BfmI	CTRYAG	4 cut(s)	321, 432, 1290, 1564
BfoI	RGCGCY	1 cut(s)	267
BfuAI	ACCTGC	1 cut(s)	379
BglII	AGATCT	3 cut(s)	14, 42, 325
BisI	GCNGC	9 cut(s)	126, 147, 385, 559, 1085, 1290, 1293, 1848, 1914
BlpI	GCTNAGC	1 cut(s)	719
BlsI	GCNGC	9 cut(s)	127, 148, 386, 560, 1086, 1291, 1294, 1849, 1915
Bme1390I	CCNGG	8 cut(s)	23, 171, 172, 519, 873, 1129, 1658, 2165
Bme18I	GGWCC	4 cut(s)	698, 851, 908, 1140
BmeRI	GACNNNNNGTC	1 cut(s)	1138
BmeT110I	CYCGRG	1 cut(s)	170
BmgBI	CACGTC	2 cut(s)	816, 1364
BmgT120I	GGNCC	5 cut(s)	698, 851, 908, 1140, 2175
BmiI	GGNNCC	7 cut(s)	634, 700, 852, 1141, 1971, 2147, 2301
BmrFI	CCNGG	8 cut(s)	23, 171, 172, 519, 873, 1129, 1658, 2165
BmrI	ACTGGG	2 cut(s)	293, 870
BmsI	GCATC	7 cut(s)	196, 429, 732, 1096, 1765, 2191, 2276
BmuI	ACTGGG	2 cut(s)	293, 870
BpiI	GAAGAC	1 cut(s)	2394
BpmI	CTGGAG	5 cut(s)	44, 207, 756, 2025, 2346
Bpu10I	CCTNAGC	1 cut(s)	51
Bpu1102I	GCTNAGC	1 cut(s)	719
BpuEI	CTTGAG	1 cut(s)	72
BpuMI	CCSGG	2 cut(s)	171, 172
BsaBI	GATNNNNATC	1 cut(s)	747
BsaHI	GRCGYC	1 cut(s)	1199
BsaI	GGTCTC	1 cut(s)	186
BsaJI	CCNNGG	8 cut(s)	170, 508, 518, 1127, 1128, 1143, 1814, 2149
BsaWI	WCCGGW	4 cut(s)	413, 485, 542, 1525
BsaXI	ACNNNNNCTCC	2 cut(s)	2291, 2321
Bsc4I	CCNNNNNNNGG	5 cut(s)	848, 860, 893, 1129, 2324
Bse118I	RCCGGY	1 cut(s)	1156
Bse1I	ACTGG	4 cut(s)	288, 773, 865, 2122
Bse3DI	GCAATG	2 cut(s)	465, 1488
Bse8I	GATNNNNATC	1 cut(s)	747
BseAI	TCCGGA	1 cut(s)	1525
BseBI	CCWGG	6 cut(s)	23, 519, 873, 1129, 1658, 2165
BseDI	CCNNGG	8 cut(s)	170, 508, 518, 1127, 1128, 1143, 1814, 2149
BseGI	GGATG	6 cut(s)	359, 420, 723, 788, 809, 2220
BseJI	GATNNNNATC	1 cut(s)	747
BseLI	CCNNNNNNNGG	5 cut(s)	848, 860, 893, 1129, 2324
BseMI	GCAATG	2 cut(s)	465, 1488
BseMII	CTCAG	2 cut(s)	42, 733
BseNI	ACTGG	4 cut(s)	288, 773, 865, 2122
BseRI	GAGGAG	8 cut(s)	204, 291, 1071, 1338, 1341, 1344, 1347, 1362
BseSI	GKGCMC	2 cut(s)	1324, 1573
BseXI	GCAGC	8 cut(s)	112, 133, 396, 1096, 1276, 1304, 1834, 1900
BseYI	CCCAGC	2 cut(s)	1552, 2184
BsgI	GTGCAG	2 cut(s)	1737, 1766
BshFI	GGCC	6 cut(s)	561, 1160, 2024, 2121, 2177, 2314
BsiHKAI	GWGCWC	4 cut(s)	30, 453, 1324, 2041
BsiHKCI	CYCGRG	1 cut(s)	170
BsiSI	CCGG	8 cut(s)	171, 414, 486, 543, 1157, 1526, 1681, 2122
BslFI	GGGAC	5 cut(s)	864, 910, 1145, 1350, 2113
BslI	CCNNNNNNNGG	5 cut(s)	848, 860, 893, 1129, 2324
BsmAI	GTCTC	2 cut(s)	186, 468
BsmFI	GGGAC	5 cut(s)	864, 910, 1145, 1350, 2113
BsmI	GAATGC	3 cut(s)	513, 1825, 2289
BsnI	GGCC	6 cut(s)	561, 1160, 2024, 2121, 2177, 2314
Bso31I	GGTCTC	1 cut(s)	186
BsoBI	CYCGRG	1 cut(s)	170
Bsp1286I	GDGCHC	6 cut(s)	30, 453, 824, 1324, 1573, 2041
Bsp13I	TCCGGA	1 cut(s)	1525
Bsp1407I	TGTACA	1 cut(s)	1894
Bsp143I	GATC	8 cut(s)	14, 42, 325, 652, 1541, 1988, 2221, 2378
Bsp1720I	GCTNAGC	1 cut(s)	719
Bsp19I	CCATGG	1 cut(s)	1814
BspACI	CCGC	5 cut(s)	558, 824, 1382, 1480, 1584
BspANI	GGCC	6 cut(s)	561, 1160, 2024, 2121, 2177, 2314
BspCNI	CTCAG	2 cut(s)	43, 732
BspEI	TCCGGA	1 cut(s)	1525
BspHI	TCATGA	1 cut(s)	2191
BspLI	GGNNCC	7 cut(s)	634, 700, 852, 1141, 1971, 2147, 2301
BspMAI	CTGCAG	1 cut(s)	1294
BspMI	ACCTGC	1 cut(s)	379
BspPI	GGATC	5 cut(s)	660, 1549, 1996, 2216, 2386
BspTNI	GGTCTC	1 cut(s)	186
BsrBI	CCGCTC	1 cut(s)	558
BsrDI	GCAATG	2 cut(s)	465, 1488
BsrFI	RCCGGY	1 cut(s)	1156
BsrGI	TGTACA	1 cut(s)	1894
BsrI	ACTGG	4 cut(s)	288, 773, 865, 2122
BssAI	RCCGGY	1 cut(s)	1156
BssECI	CCNNGG	8 cut(s)	170, 508, 518, 1127, 1128, 1143, 1814, 2149
BssMI	GATC	8 cut(s)	14, 42, 325, 652, 1541, 1988, 2221, 2378
BssNI	GRCGYC	1 cut(s)	1199
BssT1I	CCWWGG	3 cut(s)	508, 1143, 1814
Bst2UI	CCWGG	6 cut(s)	23, 519, 873, 1129, 1658, 2165
Bst4CI	ACNGT	6 cut(s)	433, 448, 901, 1463, 1715, 1961
Bst6I	CTCTTC	2 cut(s)	5, 1056
BstACI	GRCGYC	1 cut(s)	1199
BstAUI	TGTACA	1 cut(s)	1894
BstC8I	GCNNGC	8 cut(s)	158, 824, 828, 1344, 1443, 1550, 1686, 1883
BstDEI	CTNAG	8 cut(s)	51, 329, 532, 578, 662, 719, 960, 1858
BstDSI	CCRYGG	1 cut(s)	1814
BstF5I	GGATG	6 cut(s)	359, 420, 723, 788, 809, 2220
BstH2I	RGCGCY	1 cut(s)	267
BstHHI	GCGC	3 cut(s)	151, 266, 384
BstKTI	GATC	8 cut(s)	17, 45, 328, 655, 1544, 1991, 2224, 2381
BstMAI	GTCTC	2 cut(s)	186, 468
BstMBI	GATC	8 cut(s)	14, 42, 325, 652, 1541, 1988, 2221, 2378
BstMWI	GCNNNNNNNGC	5 cut(s)	157, 776, 1414, 1844, 1853
BstNI	CCWGG	6 cut(s)	23, 519, 873, 1129, 1658, 2165
BstNSI	RCATGY	2 cut(s)	1552, 1688
BstSCI	CCNGG	8 cut(s)	21, 169, 170, 517, 871, 1127, 1656, 2163
BstSFI	CTRYAG	4 cut(s)	321, 432, 1290, 1564
BstSLI	GKGCMC	2 cut(s)	1324, 1573
BstV1I	GCAGC	8 cut(s)	112, 133, 396, 1096, 1276, 1304, 1834, 1900
BstV2I	GAAGAC	1 cut(s)	2394
BstX2I	RGATCY	5 cut(s)	14, 42, 325, 652, 1988
BstYI	RGATCY	5 cut(s)	14, 42, 325, 652, 1988
BsuRI	GGCC	6 cut(s)	561, 1160, 2024, 2121, 2177, 2314
BtgI	CCRYGG	1 cut(s)	1814
BtrI	CACGTC	2 cut(s)	816, 1364
BtsCI	GGATG	6 cut(s)	359, 420, 723, 788, 809, 2220
BtsI	GCAGTG	3 cut(s)	836, 1766, 2380
BtsIMutI	CAGTG	5 cut(s)	66, 836, 1766, 2300, 2380
BveI	ACCTGC	1 cut(s)	379
Cac8I	GCNNGC	8 cut(s)	158, 824, 828, 1344, 1443, 1550, 1686, 1883
CaiI	CAGNNNCTG	1 cut(s)	431
CciI	TCATGA	1 cut(s)	2191
CfoI	GCGC	3 cut(s)	151, 266, 384
Cfr10I	RCCGGY	1 cut(s)	1156
Cfr13I	GGNCC	5 cut(s)	698, 851, 908, 1140, 2175
Cfr9I	CCCGGG	1 cut(s)	170
CseI	GACGC	1 cut(s)	1207
CsiI	ACCWGGT	1 cut(s)	1656
Csp6I	GTAC	3 cut(s)	1277, 1464, 1895
CviQI	GTAC	3 cut(s)	1277, 1464, 1895
DdeI	CTNAG	8 cut(s)	51, 329, 532, 578, 662, 719, 960, 1858
DpnI	GATC	8 cut(s)	16, 44, 327, 654, 1543, 1990, 2223, 2380
DpnII	GATC	8 cut(s)	14, 42, 325, 652, 1541, 1988, 2221, 2378
DriI	GACNNNNNGTC	1 cut(s)	1138
EaeI	YGGCCR	3 cut(s)	559, 1158, 2119
Eam1104I	CTCTTC	2 cut(s)	5, 1056
Eam1105I	GACNNNNNGTC	1 cut(s)	1138
EarI	CTCTTC	2 cut(s)	5, 1056
Ecl136II	GAGCTC	1 cut(s)	2039
Eco130I	CCWWGG	3 cut(s)	508, 1143, 1814
Eco24I	GRGCYC	2 cut(s)	824, 2041
Eco31I	GGTCTC	1 cut(s)	186
Eco32I	GATATC	1 cut(s)	1980
Eco47I	GGWCC	4 cut(s)	698, 851, 908, 1140
Eco53kI	GAGCTC	1 cut(s)	2039
Eco57I	CTGAAG	1 cut(s)	1484
Eco88I	CYCGRG	1 cut(s)	170
EcoICRI	GAGCTC	1 cut(s)	2039
EcoO109I	RGGNCCY	1 cut(s)	1140
EcoRI	GAATTC	1 cut(s)	1796
EcoRII	CCWGG	6 cut(s)	21, 517, 871, 1127, 1656, 2163
EcoRV	GATATC	1 cut(s)	1980
EcoT14I	CCWWGG	3 cut(s)	508, 1143, 1814
EcoT22I	ATGCAT	2 cut(s)	211, 2291
EcoT38I	GRGCYC	2 cut(s)	824, 2041
ErhI	CCWWGG	3 cut(s)	508, 1143, 1814
FalI	AAGNNNNNCTT	2 cut(s)	81, 113
FaqI	GGGAC	5 cut(s)	864, 910, 1145, 1350, 2113
FauI	CCCGC	1 cut(s)	831
FblI	GTMKAC	1 cut(s)	1051
Fnu4HI	GCNGC	9 cut(s)	126, 147, 385, 559, 1085, 1290, 1293, 1848, 1914
FokI	GGATG	6 cut(s)	346, 407, 710, 775, 796, 2227
FriOI	GRGCYC	2 cut(s)	824, 2041
Fsp4HI	GCNGC	9 cut(s)	126, 147, 385, 559, 1085, 1290, 1293, 1848, 1914
FspBI	CTAG	6 cut(s)	108, 114, 1019, 1538, 1581, 1626
FspI	TGCGCA	1 cut(s)	383
GlaI	GCGC	3 cut(s)	150, 265, 383
GluI	GCNGC	9 cut(s)	126, 147, 385, 559, 1085, 1290, 1293, 1848, 1914
GsaI	CCCAGC	2 cut(s)	1556, 2188
GsuI	CTGGAG	5 cut(s)	44, 207, 756, 2025, 2346
HaeII	RGCGCY	1 cut(s)	267
HaeIII	GGCC	6 cut(s)	561, 1160, 2024, 2121, 2177, 2314
HapII	CCGG	8 cut(s)	171, 414, 486, 543, 1157, 1526, 1681, 2122
HgaI	GACGC	1 cut(s)	1207
HhaI	GCGC	3 cut(s)	151, 266, 384
Hin1I	GRCGYC	1 cut(s)	1199
Hin6I	GCGC	3 cut(s)	149, 264, 382
HinP1I	GCGC	3 cut(s)	149, 264, 382
HincII	GTYRAC	3 cut(s)	444, 525, 798
HindII	GTYRAC	3 cut(s)	444, 525, 798
HinfI	GANTC	9 cut(s)	233, 500, 748, 799, 1024, 1677, 1996, 2051, 2390
HpaI	GTTAAC	1 cut(s)	444
HpaII	CCGG	8 cut(s)	171, 414, 486, 543, 1157, 1526, 1681, 2122
HphI	GGTGA	4 cut(s)	215, 299, 774, 1121
Hpy188I	TCNGA	4 cut(s)	347, 1392, 1957, 2073
Hpy99I	CGWCG	2 cut(s)	644, 820
HpyAV	CCTTC	6 cut(s)	514, 1387, 1508, 1544, 1931, 2235
HpyCH4III	ACNGT	6 cut(s)	433, 448, 901, 1463, 1715, 1961
HpyCH4IV	ACGT	4 cut(s)	642, 815, 1045, 1363
HpyF10VI	GCNNNNNNNGC	5 cut(s)	157, 776, 1414, 1844, 1853
HpyF3I	CTNAG	8 cut(s)	51, 329, 532, 578, 662, 719, 960, 1858
HpySE526I	ACGT	4 cut(s)	642, 815, 1045, 1363
Hsp92I	GRCGYC	1 cut(s)	1199
HspAI	GCGC	3 cut(s)	149, 264, 382
Kpn2I	TCCGGA	1 cut(s)	1525
KspAI	GTTAAC	1 cut(s)	444
Kzo9I	GATC	8 cut(s)	14, 42, 325, 652, 1541, 1988, 2221, 2378
LmnI	GCTCC	9 cut(s)	25, 101, 775, 1351, 1975, 2036, 2044, 2155, 2299
Lsp1109I	GCAGC	8 cut(s)	112, 133, 396, 1096, 1276, 1304, 1834, 1900
LweI	GCATC	7 cut(s)	196, 429, 732, 1096, 1765, 2191, 2276
MabI	ACCWGGT	1 cut(s)	1656
MaeI	CTAG	6 cut(s)	108, 114, 1019, 1538, 1581, 1626
MaeII	ACGT	4 cut(s)	642, 815, 1045, 1363
MaeIII	GTNAC	5 cut(s)	545, 665, 1109, 1195, 1709
MalI	GATC	8 cut(s)	16, 44, 327, 654, 1543, 1990, 2223, 2380
MbiI	CCGCTC	1 cut(s)	558
MboI	GATC	8 cut(s)	14, 42, 325, 652, 1541, 1988, 2221, 2378
MfeI	CAATTG	2 cut(s)	984, 1410
MflI	RGATCY	5 cut(s)	14, 42, 325, 652, 1988
MhlI	GDGCHC	6 cut(s)	30, 453, 824, 1324, 1573, 2041
MlyI	GAGTC	4 cut(s)	793, 2005, 2060, 2384
MmeI	TCCRAC	2 cut(s)	955, 2085
Mph1103I	ATGCAT	2 cut(s)	211, 2291
MroI	TCCGGA	1 cut(s)	1525
MroXI	GAANNNNTTC	1 cut(s)	752
MseI	TTAA	7 cut(s)	74, 132, 258, 443, 468, 482, 2114
MslI	CAYNNNNRTG	6 cut(s)	161, 458, 1689, 1788, 2073, 2398
MspI	CCGG	8 cut(s)	171, 414, 486, 543, 1157, 1526, 1681, 2122
MspR9I	CCNGG	8 cut(s)	23, 171, 172, 519, 873, 1129, 1658, 2165
MunI	CAATTG	2 cut(s)	984, 1410
Mva1269I	GAATGC	3 cut(s)	513, 1825, 2289
MvaI	CCWGG	6 cut(s)	23, 519, 873, 1129, 1658, 2165
MwoI	GCNNNNNNNGC	5 cut(s)	157, 776, 1414, 1844, 1853
NciI	CCSGG	2 cut(s)	171, 172
NcoI	CCATGG	1 cut(s)	1814
NdeII	GATC	8 cut(s)	14, 42, 325, 652, 1541, 1988, 2221, 2378
NlaIV	GGNNCC	7 cut(s)	634, 700, 852, 1141, 1971, 2147, 2301
NmuCI	GTSAC	3 cut(s)	665, 1109, 1195
NsbI	TGCGCA	1 cut(s)	383
NsiI	ATGCAT	2 cut(s)	211, 2291
NspI	RCATGY	2 cut(s)	1552, 1688
OliI	CACNNNNGTG	1 cut(s)	161
PaeI	GCATGC	2 cut(s)	1552, 1688
PagI	TCATGA	1 cut(s)	2191
PasI	CCCWGGG	1 cut(s)	1128
PcsI	WCGNNNNNNNCGW	1 cut(s)	1051
PctI	GAATGC	3 cut(s)	513, 1825, 2289
PdmI	GAANNNNTTC	1 cut(s)	752
PfeI	GAWTC	5 cut(s)	233, 500, 748, 1024, 1677
PflMI	CCANNNNNTGG	3 cut(s)	860, 893, 2324
PfoI	TCCNGGA	1 cut(s)	21
PkrI	GCNGC	9 cut(s)	127, 148, 386, 560, 1086, 1291, 1294, 1849, 1915
PleI	GAGTC	4 cut(s)	793, 2004, 2059, 2384
PpsI	GAGTC	4 cut(s)	793, 2004, 2059, 2384
PpuMI	RGGWCCY	1 cut(s)	1140
Psp124BI	GAGCTC	1 cut(s)	2041
Psp5II	RGGWCCY	1 cut(s)	1140
Psp6I	CCWGG	6 cut(s)	21, 517, 871, 1127, 1656, 2163
PspFI	CCCAGC	2 cut(s)	1552, 2184
PspGI	CCWGG	6 cut(s)	21, 517, 871, 1127, 1656, 2163
PspN4I	GGNNCC	7 cut(s)	634, 700, 852, 1141, 1971, 2147, 2301
PspPI	GGNCC	5 cut(s)	698, 851, 908, 1140, 2175
PspPPI	RGGWCCY	1 cut(s)	1140
PstI	CTGCAG	1 cut(s)	1294
PstNI	CAGNNNCTG	1 cut(s)	431
PsuI	RGATCY	5 cut(s)	14, 42, 325, 652, 1988
RsaI	GTAC	3 cut(s)	1278, 1465, 1896
RsaNI	GTAC	3 cut(s)	1277, 1464, 1895
RseI	CAYNNNNRTG	6 cut(s)	161, 458, 1689, 1788, 2073, 2398
SacI	GAGCTC	1 cut(s)	2041
SaqAI	TTAA	7 cut(s)	74, 132, 258, 443, 468, 482, 2114
SatI	GCNGC	9 cut(s)	126, 147, 385, 559, 1085, 1290, 1293, 1848, 1914
Sau3AI	GATC	8 cut(s)	14, 42, 325, 652, 1541, 1988, 2221, 2378
Sau96I	GGNCC	5 cut(s)	698, 851, 908, 1140, 2175
SchI	GAGTC	4 cut(s)	793, 2005, 2060, 2384
ScrFI	CCNGG	8 cut(s)	23, 171, 172, 519, 873, 1129, 1658, 2165
SduI	GDGCHC	6 cut(s)	30, 453, 824, 1324, 1573, 2041
SexAI	ACCWGGT	1 cut(s)	1656
SfaNI	GCATC	7 cut(s)	196, 429, 732, 1096, 1765, 2191, 2276
SfcI	CTRYAG	4 cut(s)	321, 432, 1290, 1564
SinI	GGWCC	4 cut(s)	698, 851, 908, 1140
SmaI	CCCGGG	1 cut(s)	172
SmiMI	CAYNNNNRTG	6 cut(s)	161, 458, 1689, 1788, 2073, 2398
SmlI	CTYRAG	1 cut(s)	87
SmoI	CTYRAG	1 cut(s)	87
SpeI	ACTAGT	1 cut(s)	107
SphI	GCATGC	2 cut(s)	1552, 1688
SsiI	CCGC	5 cut(s)	558, 824, 1382, 1480, 1584
SspI	AATATT	1 cut(s)	243
SspMI	CTAG	6 cut(s)	108, 114, 1019, 1538, 1581, 1626
SstI	GAGCTC	1 cut(s)	2041
StyD4I	CCNGG	8 cut(s)	21, 169, 170, 517, 871, 1127, 1656, 2163
StyI	CCWWGG	3 cut(s)	508, 1143, 1814
TaaI	ACNGT	6 cut(s)	433, 448, 901, 1463, 1715, 1961
TaiI	ACGT	4 cut(s)	645, 818, 1048, 1366
TaqI	TCGA	5 cut(s)	639, 818, 1698, 2210, 2284
TatI	WGTACW	1 cut(s)	1894
TauI	GCSGC	1 cut(s)	561
TfiI	GAWTC	5 cut(s)	233, 500, 748, 1024, 1677
Tru1I	TTAA	7 cut(s)	74, 132, 258, 443, 468, 482, 2114
Tru9I	TTAA	7 cut(s)	74, 132, 258, 443, 468, 482, 2114
TscAI	CASTG	5 cut(s)	66, 843, 1773, 2300, 2380
TseFI	GTSAC	3 cut(s)	665, 1109, 1195
TseI	GCWGC	8 cut(s)	125, 146, 384, 1084, 1289, 1292, 1847, 1913
Tsp45I	GTSAC	3 cut(s)	665, 1109, 1195
TspDTI	ATGAA	4 cut(s)	1230, 1809, 1887, 2180
TspGWI	ACGGA	2 cut(s)	1086, 1436
TspMI	CCCGGG	1 cut(s)	170
TspRI	CASTG	5 cut(s)	66, 843, 1773, 2300, 2380
Van91I	CCANNNNNTGG	3 cut(s)	860, 893, 2324
VneI	GTGCAC	1 cut(s)	1320
VpaK11BI	GGWCC	4 cut(s)	698, 851, 908, 1140
XapI	RAATTY	8 cut(s)	730, 931, 950, 1074, 1796, 1903, 2045, 2058
XceI	RCATGY	2 cut(s)	1552, 1688
XcmI	CCANNNNNNNNNTGG	1 cut(s)	843
XmaI	CCCGGG	1 cut(s)	170
XmiI	GTMKAC	1 cut(s)	1051
XmnI	GAANNNNTTC	1 cut(s)	752
XspI	CTAG	6 cut(s)	108, 114, 1019, 1538, 1581, 1626
Zsp2I	ATGCAT	2 cut(s)	211, 2291

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.