Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0008562

Physical Location & Seq

Genomic Coordinates Forward (+)

10249986 .. 10255978

Gene Structure 5993 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00088260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2403 bp

ATGAGATCAATGGGGACGAGAAGATCTGAATTGGAGTACAAGGAGCTGAAGATCTGTGAACAGGTTTTGATTGGGTTTTTATTGATTTGCTTGGTGCAAGTTTCGCTTGGAGCTACTAATATGCAAGATGTTGCGGCGATTAACAGCTTACATGCTGCACTCGGCACGCCCGTTCTTCCTGGGTGGACTTCTGCTGGGGGAGACCCTTGTGGGGAAGCATGGCAAGGGGTTCAGTGCAATGACAGCACAATTATATCAATAACTCTTAATGCTGCCAATTTGGGAGGAGAATTGGGTGAGAACTTAGGATCGTTCACTTCAATGAGAACAATAGATCTGAGCAACAATCATATTGGTGGAAGTATACCTTCCATTTTACCTGTTACCATGCGAACCTTTTTTCTTTCAGCTAATCAATTGACCGGAAGTGTCCCCATTTCTTTGGCTTCATTGGTTGAGCTCACAGATATGTCTCTTAACAATAATCTCTTAACTGGTGAACTACCAGATGTGTTTGAAAGCCTTGCAGCGTTGATCAATTTAGATTTGTCCGGTAACAATTTGAGTGGGCACCTGCCACCCTCTCTAGAAAACGCTAAAGCTTTGACCAGTCTACATTTGCAGAACAATCAGCTATCTGGAACACTTGACATGTTACAGGATCTTCCCTTGACCGATCTGAATATAGAGAATAACCTATTTGCTGGACCCATTCCTCCAAAGTTGCTGAGCATCCCAAATTTTAGAAGTGATGGAAATCCATTAAATTCGTCTATTGCTCCAGTCCCTCCACCCACATCTCCGTTGGCTCCGTCACCTCCTTCGACTACACCACCATCTCCTTATGCCTTTTGGGGACCACCTTCTGGAAAAACACCACCTGGTAAAACACCACCTGGAACACCACCCGCATCTGCACAAGAAAAATCAAATTCTGGAAAAGCAAATAGCTTTTTTACTACTAAAAGGGTAGTTGGGATATCAATTGGAGGGGTGTTTGTACTTATTATTTTGGTACTAGTAGTTTTGTTTCTTATGCCAAGAGGTACCAGACGAAGAGGAGAGTCTGACAGAATTTCCAAGCCACATCAAATAGGAGCTTATCGCGACAGGGAGAATCCACCCATGGATAATGGGTCCTTGGTTCAACCAAACAATCAATTACAGAAAGTTCCAAAAGAGGAAGTTATGAGGCCCAAAGAAGATAATGAAACAGAACCCCGAAGACCATGGGCAATGCCAAAGTCAAATGATGAGCAAGATAGAAATGTCCAAAGAATGAGTGCAGTACCCAAACAACGAGATGATCATGTGGTTGATATGAGTCGACTTAATTCGTTTTTGATGCCTCCTCCCCCTCCTCCTCCGCCACCCCCTCCTCCACCACCATTTCCTGATGAGACGGTCACTGTAAAGCCAATTGTACCAACTGAAGTAAAGACAGTGAAGCCTTCCACCAGAAGTCTAAACCTCACAATTTCTGCCAAGTCTTTCACCATTGCTTCACTTCAGCAATATACAAACAGCTTTTCTCAGGATAATCTTATAGGAGCGGGCATGCTGGGAAGTGTCTATAGGGCACAGCTTCCTGATGGAAAGCTCCTTGCGGTCAAGAAATTGGACAAGAGAGCTTCCAATCAGCAGAAGGATGAGGAGTTTCTTGAACTGGTAAACAACATTGATAGAATCCGGCATGCTAATGTTGTAGAGCTCATAGGTTACTGTGCAGAGCATGGTCAAAGGCTTCTTATCTACGAGTATTGCAGTAATGGTTCACTGCATGATACTCTGCACTTGGAGGATGAATTTAAGAAGAAACTATCGTGGAATACTCGCATAAGAATGGCACTAGGAGCTGCAAGAGCTTTGGAGTATTTGCATGAGGTCTGTCAGCCACGTATTGTACACAGGAATTTCAAGTCTAACAACCTTCTCCTTGATGATGACCTTTCTATCCGTGTTTCTGACTGTGGCTTGGCTTCCTTAATATCATCTGGCTCCGTAAGTCAGTTGTCAGGGCATCTGCTATCAGCTTACGGTTACGGAGCTCCAGAATTTGATTCAGGAATTTATACTCATCAGAGTGATGTTTACAGCTTTGGTGTAGTGATGTTAGAACTATTAACAGGCCGAAAATCCCATGACAGGACACGGACTCGAGCGGAGCAATTCCTGGTTAGATGGGCCATTCCCCAGCTTCATGACATTGATGCATTATCAAGGATGGTCGATCCCTCTCTAAACAGAGAATATCCTACAAAGTCCTTATCACACTTCGCTGACATTATATCTCGATGTGTTCAGTCGGAGCCAGAGTTCAGGCCGCCAATGTCTGAAGTTGTTCAGGACCTATTAGACATGATACGAAGAGAGCCTCGCGGCAGCGGATCAAGTGAAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007623 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030054 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048511 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_8 Pkinase_Tyr

Protein Analysis

800

Amino Acids

87.64

Weight (kDa)

6.06

Isoelectric Point (pI)

42.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	511 - 783	1.1e-38	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	513 - 784	1.6e-44	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008562)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03390 AT4G03390
fragaria_vesca	FvH4_6g44950 FvH4_6g44950 FvH4_6g44950
malus_domestica	MD09G1091500.v1.1 MD17G1081400.v1.1
prunus_persica	Prupe.3G233500_v2.0.a1 Prupe.3G233500_v2.0.a1 Prupe.3G233500_v2.0.a1 Prupe.3G233500_v2.0.a1 Prupe.3G233500_v2.0.a1 Prupe.3G233500_v2.0.a1
pyrus_communis	pycom09g01720 pycom17g07970
rosa_chinensis	RchiOBHm_Chr2g0162181
rosa_laevigata	RLG00000021326
rosa_multiflora	Rmu_sc0004020.1_g000030
rosa_roxburghii	Rroxscaffold_2G00088260
rosa_rugosa	Rorug02G0495000 Rorug02G0495100
rosa_samantha	Rh2BG574600
rosa_wichuraiana	Rw2G046500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	582
Acc36I	ACCTGC	1 cut(s)	582
Acc65I	GGTACC	1 cut(s)	1046
AccB1I	GGYRCC	2 cut(s)	570, 1046
AccB7I	CCANNNNNTGG	1 cut(s)	866
AccBSI	CCGCTC	2 cut(s)	1553, 2162
AccI	GTMKAC	3 cut(s)	364, 613, 1327
AccII	CGCG	2 cut(s)	1107, 2379
AciI	CCGC	9 cut(s)	134, 909, 1367, 1553, 1607, 2162, 2324, 2379, 2385
AclWI	GGATC	4 cut(s)	316, 669, 2225, 2395
AcsI	RAATTY	8 cut(s)	739, 766, 931, 1074, 1805, 1912, 2054, 2067
AcuI	CTGAAG	4 cut(s)	68, 1452, 1493, 2355
AfaI	GTAC	7 cut(s)	38, 1002, 1017, 1048, 1290, 1425, 1905
AfiI	CCNNNNNNNGG	3 cut(s)	211, 866, 2145
AflIII	ACRYGT	1 cut(s)	651
AgsI	TTSAA	5 cut(s)	321, 518, 1148, 1664, 1918
AhlI	ACTAGT	1 cut(s)	1018
AjnI	CCWGG	4 cut(s)	178, 880, 895, 2172
AloI	GAACNNNNNNTCC	2 cut(s)	2300, 2332
Alw21I	GWGCWC	3 cut(s)	462, 1713, 2050
Alw26I	GTCTC	3 cut(s)	195, 477, 1394
AlwI	GGATC	4 cut(s)	316, 669, 2225, 2395
Ama87I	CYCGRG	1 cut(s)	2157
AoxI	GGCC	4 cut(s)	1193, 2128, 2184, 2321
ApeKI	GCWGC	5 cut(s)	155, 272, 527, 1856, 2382
ApoI	RAATTY	8 cut(s)	739, 766, 931, 1074, 1805, 1912, 2054, 2067
Asp700I	GAANNNNTTC	1 cut(s)	2340
Asp718I	GGTACC	1 cut(s)	1046
AspS9I	GGNCC	6 cut(s)	707, 857, 1137, 1194, 2184, 2347
AsuHPI	GGTGA	4 cut(s)	308, 509, 807, 1486
AvaI	CYCGRG	1 cut(s)	2157
AvaII	GGWCC	4 cut(s)	707, 857, 1137, 2347
BaeGI	GKGCMC	2 cut(s)	573, 1582
BanI	GGYRCC	2 cut(s)	570, 1046
BanII	GRGCYC	3 cut(s)	462, 1713, 2050
BbsI	GAAGAC	1 cut(s)	1231
Bbv12I	GWGCWC	3 cut(s)	462, 1713, 2050
BbvI	GCAGC	5 cut(s)	142, 259, 539, 1843, 2394
BccI	CCATC	5 cut(s)	746, 844, 1586, 2175, 2218
BciT130I	CCWGG	4 cut(s)	180, 882, 897, 2174
BclI	TGATCA	2 cut(s)	534, 1306
BcoDI	GTCTC	3 cut(s)	195, 477, 1394
BcuI	ACTAGT	1 cut(s)	1018
BfaI	CTAG	3 cut(s)	587, 1019, 1850
BfmI	CTRYAG	1 cut(s)	1573
BfuAI	ACCTGC	1 cut(s)	582
BglII	AGATCT	3 cut(s)	23, 51, 334
BisI	GCNGC	8 cut(s)	135, 156, 273, 528, 1857, 2324, 2380, 2383
BlpI	GCTNAGC	1 cut(s)	728
BlsI	GCNGC	8 cut(s)	136, 157, 274, 529, 1858, 2325, 2381, 2384
Bme1390I	CCNGG	4 cut(s)	180, 882, 897, 2174
Bme18I	GGWCC	4 cut(s)	707, 857, 1137, 2347
BmeT110I	CYCGRG	1 cut(s)	2157
BmgT120I	GGNCC	6 cut(s)	707, 857, 1137, 1194, 2184, 2347
BmiI	GGNNCC	8 cut(s)	572, 709, 810, 858, 1048, 1138, 1999, 2310
BmrFI	CCNGG	4 cut(s)	180, 882, 897, 2174
BmsI	GCATC	5 cut(s)	741, 920, 1335, 2029, 2200
BpiI	GAAGAC	1 cut(s)	1231
BpmI	CTGGAG	2 cut(s)	765, 2034
Bpu1102I	GCTNAGC	1 cut(s)	728
BsaAI	YACGTR	1 cut(s)	1898
BsaBI	GATNNNNATC	1 cut(s)	756
BsaI	GGTCTC	1 cut(s)	195
BsaJI	CCNNGG	4 cut(s)	179, 1125, 1140, 1229
BsaWI	WCCGGW	2 cut(s)	422, 551
BsaXI	ACNNNNNCTCC	2 cut(s)	2300, 2330
Bsc4I	CCNNNNNNNGG	3 cut(s)	211, 866, 2145
Bse1I	ACTGG	4 cut(s)	499, 609, 782, 1671
Bse3DI	GCAATG	3 cut(s)	244, 1242, 1497
Bse8I	GATNNNNATC	1 cut(s)	756
BseBI	CCWGG	4 cut(s)	180, 882, 897, 2174
BseDI	CCNNGG	4 cut(s)	179, 1125, 1140, 1229
BseGI	GGATG	4 cut(s)	732, 1654, 1807, 2229
BseJI	GATNNNNATC	1 cut(s)	756
BseLI	CCNNNNNNNGG	3 cut(s)	211, 866, 2145
BseMI	GCAATG	3 cut(s)	244, 1242, 1497
BseMII	CTCAG	3 cut(s)	329, 719, 1547
BseNI	ACTGG	4 cut(s)	499, 609, 782, 1671
BseRI	GAGGAG	7 cut(s)	300, 1074, 1341, 1350, 1353, 1368, 1667
BseSI	GKGCMC	2 cut(s)	573, 1582
BseXI	GCAGC	5 cut(s)	142, 259, 539, 1843, 2394
BseYI	CCCAGC	3 cut(s)	194, 1561, 2193
BsgI	GTGCAG	5 cut(s)	141, 900, 1305, 1746, 1775
Bsh1236I	CGCG	2 cut(s)	1107, 2379
BshFI	GGCC	4 cut(s)	1195, 2130, 2186, 2323
BshNI	GGYRCC	2 cut(s)	570, 1046
BsiHKAI	GWGCWC	3 cut(s)	462, 1713, 2050
BsiHKCI	CYCGRG	1 cut(s)	2157
BsiSI	CCGG	3 cut(s)	423, 552, 1690
BslFI	GGGAC	4 cut(s)	28, 416, 770, 870
BslI	CCNNNNNNNGG	3 cut(s)	211, 866, 2145
BsmAI	GTCTC	3 cut(s)	195, 477, 1394
BsmBI	CGTCTC	1 cut(s)	1394
BsmFI	GGGAC	4 cut(s)	28, 416, 770, 870
BsnI	GGCC	4 cut(s)	1195, 2130, 2186, 2323
Bso31I	GGTCTC	1 cut(s)	195
BsoBI	CYCGRG	1 cut(s)	2157
Bsp1286I	GDGCHC	5 cut(s)	462, 573, 1582, 1713, 2050
Bsp1407I	TGTACA	1 cut(s)	1903
Bsp1720I	GCTNAGC	1 cut(s)	728
Bsp19I	CCATGG	2 cut(s)	1125, 1229
Bsp68I	TCGCGA	1 cut(s)	1107
BspACI	CCGC	9 cut(s)	134, 909, 1367, 1553, 1607, 2162, 2324, 2379, 2385
BspANI	GGCC	4 cut(s)	1195, 2130, 2186, 2323
BspCNI	CTCAG	3 cut(s)	330, 720, 1546
BspFNI	CGCG	2 cut(s)	1107, 2379
BspHI	TCATGA	1 cut(s)	2200
BspLI	GGNNCC	8 cut(s)	572, 709, 810, 858, 1048, 1138, 1999, 2310
BspMI	ACCTGC	1 cut(s)	582
BspPI	GGATC	4 cut(s)	316, 669, 2225, 2395
BspT107I	GGYRCC	2 cut(s)	570, 1046
BspTNI	GGTCTC	1 cut(s)	195
BsrBI	CCGCTC	2 cut(s)	1553, 2162
BsrDI	GCAATG	3 cut(s)	244, 1242, 1497
BsrGI	TGTACA	1 cut(s)	1903
BsrI	ACTGG	4 cut(s)	499, 609, 782, 1671
BssECI	CCNNGG	4 cut(s)	179, 1125, 1140, 1229
BssNAI	GTATAC	1 cut(s)	365
BssT1I	CCWWGG	3 cut(s)	1125, 1140, 1229
Bst1107I	GTATAC	1 cut(s)	365
Bst2UI	CCWGG	4 cut(s)	180, 882, 897, 2174
Bst4CI	ACNGT	6 cut(s)	1405, 1411, 1444, 1724, 1970, 2039
Bst6I	CTCTTC	2 cut(s)	1051, 2362
BstAUI	TGTACA	1 cut(s)	1903
BstBAI	YACGTR	1 cut(s)	1898
BstC8I	GCNNGC	4 cut(s)	167, 1555, 1559, 1695
BstDEI	CTNAG	4 cut(s)	304, 338, 728, 1533
BstDSI	CCRYGG	2 cut(s)	1125, 1229
BstF5I	GGATG	4 cut(s)	732, 1654, 1807, 2229
BstFNI	CGCG	2 cut(s)	1107, 2379
BstMAI	GTCTC	3 cut(s)	195, 477, 1394
BstMWI	GCNNNNNNNGC	4 cut(s)	103, 243, 1853, 1862
BstNI	CCWGG	4 cut(s)	180, 882, 897, 2174
BstNSI	RCATGY	4 cut(s)	155, 655, 1561, 1697
BstSCI	CCNGG	4 cut(s)	178, 880, 895, 2172
BstSFI	CTRYAG	1 cut(s)	1573
BstSLI	GKGCMC	2 cut(s)	573, 1582
BstUI	CGCG	2 cut(s)	1107, 2379
BstV1I	GCAGC	5 cut(s)	142, 259, 539, 1843, 2394
BstV2I	GAAGAC	1 cut(s)	1231
BstX2I	RGATCY	4 cut(s)	23, 51, 334, 661
BstXI	CCANNNNNNTGG	1 cut(s)	442
BstYI	RGATCY	4 cut(s)	23, 51, 334, 661
BstZ17I	GTATAC	1 cut(s)	365
BsuRI	GGCC	4 cut(s)	1195, 2130, 2186, 2323
BtgI	CCRYGG	2 cut(s)	1125, 1229
BtsCI	GGATG	4 cut(s)	732, 1654, 1807, 2229
BtsI	GCAGTG	1 cut(s)	1775
BtsIMutI	CAGTG	4 cut(s)	239, 1407, 1449, 1775
BtuMI	TCGCGA	1 cut(s)	1107
BveI	ACCTGC	1 cut(s)	582
Cac8I	GCNNGC	4 cut(s)	167, 1555, 1559, 1695
CciI	TCATGA	1 cut(s)	2200
Cfr13I	GGNCC	6 cut(s)	707, 857, 1137, 1194, 2184, 2347
CsiI	ACCWGGT	1 cut(s)	880
Csp6I	GTAC	7 cut(s)	37, 1001, 1016, 1047, 1289, 1424, 1904
CspCI	CAANNNNNGTGG	2 cut(s)	547, 582
CviQI	GTAC	7 cut(s)	37, 1001, 1016, 1047, 1289, 1424, 1904
DdeI	CTNAG	4 cut(s)	304, 338, 728, 1533
Eam1104I	CTCTTC	2 cut(s)	1051, 2362
EarI	CTCTTC	2 cut(s)	1051, 2362
EciI	GGCGGA	1 cut(s)	1356
Ecl136II	GAGCTC	3 cut(s)	460, 1711, 2048
Eco130I	CCWWGG	3 cut(s)	1125, 1140, 1229
Eco24I	GRGCYC	3 cut(s)	462, 1713, 2050
Eco31I	GGTCTC	1 cut(s)	195
Eco32I	GATATC	1 cut(s)	981
Eco47I	GGWCC	4 cut(s)	707, 857, 1137, 2347
Eco53kI	GAGCTC	3 cut(s)	460, 1711, 2048
Eco57I	CTGAAG	4 cut(s)	68, 1452, 1493, 2355
Eco88I	CYCGRG	1 cut(s)	2157
EcoICRI	GAGCTC	3 cut(s)	460, 1711, 2048
EcoO109I	RGGNCCY	2 cut(s)	1137, 2347
EcoRII	CCWGG	4 cut(s)	178, 880, 895, 2172
EcoRV	GATATC	1 cut(s)	981
EcoT14I	CCWWGG	3 cut(s)	1125, 1140, 1229
EcoT22I	ATGCAT	1 cut(s)	2215
EcoT38I	GRGCYC	3 cut(s)	462, 1713, 2050
ErhI	CCWWGG	3 cut(s)	1125, 1140, 1229
Esp3I	CGTCTC	1 cut(s)	1394
FalI	AAGNNNNNCTT	4 cut(s)	90, 122, 352, 384
FaqI	GGGAC	4 cut(s)	28, 416, 770, 870
FauI	CCCGC	2 cut(s)	916, 1546
FbaI	TGATCA	2 cut(s)	534, 1306
FblI	GTMKAC	3 cut(s)	364, 613, 1327
Fnu4HI	GCNGC	8 cut(s)	135, 156, 273, 528, 1857, 2324, 2380, 2383
FokI	GGATG	4 cut(s)	719, 1661, 1814, 2236
FriOI	GRGCYC	3 cut(s)	462, 1713, 2050
Fsp4HI	GCNGC	8 cut(s)	135, 156, 273, 528, 1857, 2324, 2380, 2383
FspBI	CTAG	3 cut(s)	587, 1019, 1850
GluI	GCNGC	8 cut(s)	135, 156, 273, 528, 1857, 2324, 2380, 2383
GsaI	CCCAGC	3 cut(s)	198, 1565, 2197
GsuI	CTGGAG	2 cut(s)	765, 2034
HaeIII	GGCC	4 cut(s)	1195, 2130, 2186, 2323
HapII	CCGG	3 cut(s)	423, 552, 1690
HincII	GTYRAC	1 cut(s)	1328
HindII	GTYRAC	1 cut(s)	1328
HindIII	AAGCTT	1 cut(s)	600
HinfI	GANTC	6 cut(s)	1064, 1117, 1324, 1686, 2060, 2155
HpaII	CCGG	3 cut(s)	423, 552, 1690
HphI	GGTGA	4 cut(s)	308, 509, 807, 1486
Hpy188I	TCNGA	8 cut(s)	28, 339, 681, 1069, 1966, 2082, 2308, 2335
HpyAV	CCTTC	6 cut(s)	378, 831, 873, 1461, 1639, 1940
HpyCH4III	ACNGT	6 cut(s)	1405, 1411, 1444, 1724, 1970, 2039
HpyCH4IV	ACGT	1 cut(s)	1897
HpyF10VI	GCNNNNNNNGC	4 cut(s)	103, 243, 1853, 1862
HpyF3I	CTNAG	4 cut(s)	304, 338, 728, 1533
HpySE526I	ACGT	1 cut(s)	1897
KpnI	GGTACC	1 cut(s)	1050
Ksp22I	TGATCA	2 cut(s)	534, 1306
Lsp1109I	GCAGC	5 cut(s)	142, 259, 539, 1843, 2394
LweI	GCATC	5 cut(s)	741, 920, 1335, 2029, 2200
MabI	ACCWGGT	1 cut(s)	880
MaeI	CTAG	3 cut(s)	587, 1019, 1850
MaeII	ACGT	1 cut(s)	1897
MaeIII	GTNAC	7 cut(s)	382, 554, 654, 813, 1405, 1718, 2039
MbiI	CCGCTC	2 cut(s)	1553, 2162
MboII	GAAGA	9 cut(s)	33, 61, 167, 656, 1068, 1214, 1236, 1825, 2379
MfeI	CAATTG	3 cut(s)	416, 984, 1419
MflI	RGATCY	4 cut(s)	23, 51, 334, 661
MhlI	GDGCHC	5 cut(s)	462, 573, 1582, 1713, 2050
MlyI	GAGTC	3 cut(s)	1073, 1333, 2149
MmeI	TCCRAC	1 cut(s)	2286
Mph1103I	ATGCAT	1 cut(s)	2215
MroXI	GAANNNNTTC	1 cut(s)	2340
MseI	TTAA	9 cut(s)	141, 267, 477, 491, 764, 1332, 1809, 1985, 2123
MslI	CAYNNNNRTG	6 cut(s)	320, 354, 467, 1698, 1841, 2082
MspA1I	CMGCKG	1 cut(s)	2385
MspI	CCGG	3 cut(s)	423, 552, 1690
MspR9I	CCNGG	4 cut(s)	180, 882, 897, 2174
MunI	CAATTG	3 cut(s)	416, 984, 1419
MvaI	CCWGG	4 cut(s)	180, 882, 897, 2174
MvnI	CGCG	2 cut(s)	1107, 2379
MwoI	GCNNNNNNNGC	4 cut(s)	103, 243, 1853, 1862
NcoI	CCATGG	2 cut(s)	1125, 1229
NlaIV	GGNNCC	8 cut(s)	572, 709, 810, 858, 1048, 1138, 1999, 2310
NmeAIII	GCCGAG	1 cut(s)	141
NmuCI	GTSAC	2 cut(s)	813, 1405
NruI	TCGCGA	1 cut(s)	1107
NsiI	ATGCAT	1 cut(s)	2215
NspI	RCATGY	4 cut(s)	155, 655, 1561, 1697
PaeI	GCATGC	2 cut(s)	1561, 1697
PaeR7I	CTCGAG	1 cut(s)	2157
PagI	TCATGA	1 cut(s)	2200
PaqCI	CACCTGC	1 cut(s)	582
PciI	ACATGT	1 cut(s)	651
PcsI	WCGNNNNNNNCGW	1 cut(s)	168
PdmI	GAANNNNTTC	1 cut(s)	2340
PfeI	GAWTC	3 cut(s)	1117, 1686, 2060
PflMI	CCANNNNNTGG	1 cut(s)	866
PkrI	GCNGC	8 cut(s)	136, 157, 274, 529, 1858, 2325, 2381, 2384
PleI	GAGTC	3 cut(s)	1072, 1332, 2149
PpsI	GAGTC	3 cut(s)	1072, 1332, 2149
Ppu21I	YACGTR	1 cut(s)	1898
PpuMI	RGGWCCY	2 cut(s)	1137, 2347
PscI	ACATGT	1 cut(s)	651
Psp124BI	GAGCTC	3 cut(s)	462, 1713, 2050
Psp5II	RGGWCCY	2 cut(s)	1137, 2347
Psp6I	CCWGG	4 cut(s)	178, 880, 895, 2172
PspFI	CCCAGC	3 cut(s)	194, 1561, 2193
PspGI	CCWGG	4 cut(s)	178, 880, 895, 2172
PspN4I	GGNNCC	8 cut(s)	572, 709, 810, 858, 1048, 1138, 1999, 2310
PspPI	GGNCC	6 cut(s)	707, 857, 1137, 1194, 2184, 2347
PspPPI	RGGWCCY	2 cut(s)	1137, 2347
PspXI	VCTCGAGB	1 cut(s)	2157
PsuI	RGATCY	4 cut(s)	23, 51, 334, 661
RruI	TCGCGA	1 cut(s)	1107
RsaI	GTAC	7 cut(s)	38, 1002, 1017, 1048, 1290, 1425, 1905
RsaNI	GTAC	7 cut(s)	37, 1001, 1016, 1047, 1289, 1424, 1904
RseI	CAYNNNNRTG	6 cut(s)	320, 354, 467, 1698, 1841, 2082
SacI	GAGCTC	3 cut(s)	462, 1713, 2050
SalI	GTCGAC	1 cut(s)	1326
SaqAI	TTAA	9 cut(s)	141, 267, 477, 491, 764, 1332, 1809, 1985, 2123
SatI	GCNGC	8 cut(s)	135, 156, 273, 528, 1857, 2324, 2380, 2383
Sau96I	GGNCC	6 cut(s)	707, 857, 1137, 1194, 2184, 2347
SchI	GAGTC	3 cut(s)	1073, 1333, 2149
ScrFI	CCNGG	4 cut(s)	180, 882, 897, 2174
SduI	GDGCHC	5 cut(s)	462, 573, 1582, 1713, 2050
SexAI	ACCWGGT	1 cut(s)	880
SfaNI	GCATC	5 cut(s)	741, 920, 1335, 2029, 2200
SfcI	CTRYAG	1 cut(s)	1573
Sfr274I	CTCGAG	1 cut(s)	2157
SinI	GGWCC	4 cut(s)	707, 857, 1137, 2347
SlaI	CTCGAG	1 cut(s)	2157
SmiMI	CAYNNNNRTG	6 cut(s)	320, 354, 467, 1698, 1841, 2082
SmlI	CTYRAG	1 cut(s)	2157
SmoI	CTYRAG	1 cut(s)	2157
SpeI	ACTAGT	1 cut(s)	1018
SphI	GCATGC	2 cut(s)	1561, 1697
SsiI	CCGC	9 cut(s)	134, 909, 1367, 1553, 1607, 2162, 2324, 2379, 2385
SspMI	CTAG	3 cut(s)	587, 1019, 1850
SstI	GAGCTC	3 cut(s)	462, 1713, 2050
StyD4I	CCNGG	4 cut(s)	178, 880, 895, 2172
StyI	CCWWGG	3 cut(s)	1125, 1140, 1229
TaaI	ACNGT	6 cut(s)	1405, 1411, 1444, 1724, 1970, 2039
TaiI	ACGT	1 cut(s)	1900
TaqI	TCGA	5 cut(s)	824, 1327, 2158, 2229, 2293
TaqII	GACCGA	1 cut(s)	689
TatI	WGTACW	3 cut(s)	36, 1000, 1903
TauI	GCSGC	3 cut(s)	137, 2326, 2382
TfiI	GAWTC	3 cut(s)	1117, 1686, 2060
Tru1I	TTAA	9 cut(s)	141, 267, 477, 491, 764, 1332, 1809, 1985, 2123
Tru9I	TTAA	9 cut(s)	141, 267, 477, 491, 764, 1332, 1809, 1985, 2123
TscAI	CASTG	4 cut(s)	239, 1414, 1449, 1782
TseFI	GTSAC	2 cut(s)	813, 1405
TseI	GCWGC	5 cut(s)	155, 272, 527, 1856, 2382
Tsp45I	GTSAC	2 cut(s)	813, 1405
TspDTI	ATGAA	4 cut(s)	438, 1224, 1818, 2189
TspGWI	ACGGA	6 cut(s)	792, 801, 1946, 1990, 2058, 2167
TspRI	CASTG	4 cut(s)	239, 1414, 1449, 1782
Van91I	CCANNNNNTGG	1 cut(s)	866
VpaK11BI	GGWCC	4 cut(s)	707, 857, 1137, 2347
XapI	RAATTY	8 cut(s)	739, 766, 931, 1074, 1805, 1912, 2054, 2067
XbaI	TCTAGA	1 cut(s)	586
XceI	RCATGY	4 cut(s)	155, 655, 1561, 1697
XcmI	CCANNNNNNNNNTGG	1 cut(s)	802
XhoI	CTCGAG	1 cut(s)	2157
XmiI	GTMKAC	3 cut(s)	364, 613, 1327
XmnI	GAANNNNTTC	1 cut(s)	2340
XspI	CTAG	3 cut(s)	587, 1019, 1850
Zsp2I	ATGCAT	1 cut(s)	2215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.