Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0014465

Physical Location & Seq

Genomic Coordinates Forward (+)

6717726 .. 6720725

Gene Structure 3000 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1091700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3000 bp

ATGGAATTAGGCCAAGAAAGTTTCCAGAGCTCAATTCACAGCCGCACTTTTTCACCAAGTAAGCTTCGCAACCTTCATCATCATCAATGTCTTTTCGGAGCTAGGAATTTTCTAAAATTTAACTTACCCAATTCTCCGAATGTCGAAAACATTGGGAATTCACGAGCTCAGAAGCAGAATTTGAGAGGTTCTAGGGTTTTGGTAGGGTTTAAGCTTCAGTGTCATTCTAAATCGTTGGTTTTTCCCACGAAAGTTTCTTCTATTAATGGTAAGAAGAAGGGGTATGGAGGCGTGTTGCCTTCGATTTTGCGATCTCTAGAGTCCCAAAAAGATGTTGAAAAGACACTTAATTCGTTAGGTGAAAATTTGAATCCGAAAGAACAGACTGTTATTCTCAAGGAACAAAGGAGCTGGGAGAGGGTTTTGCGCGTTTTCGAGTGGTTCAAGTCACAGAAAGAGTATGTTCCCAATGTAATACATTATAATGTGGTGCTTCGGAAACTGGGTAGGGCTCAGAAGTGGGATGAATTGAGGCTTTGTTGGATTGAAATGGCGAAAAGGGGTGTTCTGCCAACAAACAACACATATGCAATGCTCGTTGATGTGTATGGGAAAGCAGGCCTTGTGAAGGAAGCACTTTTGTGGATTAAGCACATGAAACTGAGGGGAATGTTTCCGGATGATGTTACCATGAATACAGTTGTTCGGGCATTGAAGGATGCAGGAGAGTTTGATAGGGCAGACAAGTTTTATAAAGATTGGTGCACTGGGAAGATTGAGCTCGATGAGCTCGATTTGGATTCTATGGGTGACTCTGTAAATGGTTCTGACTCAGAGCCTATTAGTTTTAAGCATTTCTTGTCCACTGAACTTTTCAAGACAGGTGGGAGAATTCCCACGTCAAAGATTACGACCTCATTGGATACACAGAACTCTGATCGAAAACCACGACAGGCATCTACATATAATGCTTTGATTGATTTGTATGGGAAGGCAGGGCGTCTTGATGATGCTGCTAATGTGTTTGGAGAAATGATGAAGTCTGGGGTGCCAATGGATGTTATAACTTTCAACACTATGATCTTTACTTGTGGAAGTCATGGTCATTTGTCGGAAGCGGAAACTTTGCTTAGTAAGATGGAAGAACGTGGAATTTCTCCTGACACGAGAACTTATAACATTTTTCTATCTCTGTATGCTGATGTGGGGAACATTGATGCTGCTCTGAACTGTTATACAAAGATCAGACAGGTGGGTCTTTCCCCAGATATTGTATCTCACAGGACTATTCTTCATGTGTTATGTGAGAGAAACATGGTCCGAGAAGTGGAGAATGTGATTCAGGATATGGAGAAATCTGGTGTGCGTGTCGATGAGCATTCTGTTCCTGGTGTCATCAAGATGTATATTAATGAAGGACAGCTTGTTCGGGCAAAGTTGTTTTACGAGAAATGTCAGTTGAATGGTGAGCTGTCATCAAGGACATGTGCAGCAATTATAGATGCATATGCTGAAAAGAGATTTTGGACTGAAGCTGAGGCAGTATTCTATAGGAAGAAAGACTTGGTAAGACAGAAGAAGGATGTTGTGGAGTATAACGTCATGATCAAAGCTTATGGCAAGGCAAAGCTTTATGATAAAGCATTTTCTCTCTTCAAAGGCATGAGAAATCATGGGACTTGGCCCGATAACTGCACATATAATTCTCTCATTCAGATGTTCTCAGGAGGTGATTTAGTGGACCAGGCAAGGGATGTTTTAACTGAAATGCGGGAAATGGGTTTTAAGCCGCACTCTCTAGCCTTCTCTGCTCTTATTGCATGCTGTGCTCGCCTTGGCCAGCTTTCTGATGCAGTTGATGTGTACCAAGACTTGGTAAATTCGGGGGTCAAACCAAATGAATTTGTATATGGTTCTTTGATCAACGGATTTGTAGAAACTGGCAGAGTTGAGGAAGCATTAAAGTATTTTCGGCACATGGAAGAATCTGGGATTTCTGCAAATCAAATAGTTCTTACTTCCCTCATAAAGGCATATGGTAAGGTGGACTCCCTTGATGGAGCGAAAGTACTCTATGAGAAGTTGAAAGATCTAGAGGGTGCCCTAGATATTGTTGCATCTAACAGCATGATCGATCTCTATGCAGATCGTGGGATGGTGACTGAAGCCGAATTGATTTTTGAAAAACTGAGAGCCAAGGGTTGGGCAAATGAGATAACATATGCGACCATGATATATCTTTACAAGAGTGTGGGCATGCTTGATGAAGCCATTGATATAGCAGAGGAAATGAAACTGTCAGGTCTAGTGAGGGACTGCGGTTCATTTGACAAGGTAATGTCATGTTATGCCATTAACGGGCAGCTACGGGAATGTGGCGAATTATTGCATGAGATGGTAACTCGGAAACTCTTGCCAGACATCGGGACTTTTAAAATATTATTCACGATATTGAAAAAAGGTGTTTCCATTGAAGCAGTAACGCAGTTACAGTCATCCTACCATGAAGGGAAACCTTACTCTAGGCAAGCTATCATCACTTCTGTCTTCTCTATGGTGGGTATGCATGCTTTGGCGCTCGAGTCCTGTGAAAAATTCACAAAAGCTGATGTAAAGCTTGATTCTTTTCTCTGTAATGTTGCAATACATGCTTATGGGGCGGCTGGGGAGATCGACAAGGCTCTGAACATGTTCATGAGAATGCAGGATGAAAACTTGGAACCAGACGTTGTAACGTATATTAACCTAGTACGTTGCTATGGAAAAGCTGGTATGCTTGAAGGTGTGAAGCGAATATACAGCCAGCTGAAATATGAAGAGACAGAGCCAAATCATTCCTTGTACAAAGCCGTTCTTGATGCCTATACAGATGCCAACAGGCATGACCTTGCCAAATTGGTTAGCCAAGAGATGAGATACGCGTTCTACTCAGACCATCAGACGGGTTCCGAAACTAAAGACGAGTCTGCTGAGGCAACTTCAGAGCTAGAGGATTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

1000

Amino Acids

112.74

Weight (kDa)

6.49

Isoelectric Point (pI)

29.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	156 - 203	1.1e-06	PPR repeat family
PPR_long	PF17177	317 - 478	4.4e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	320 - 362	2.8e-12	PPR repeat family
PPR	PF01535	320 - 350	4.2e-08	PPR repeat
PPR_1	PF12854	321 - 345	1.1e-06	PPR repeat
PPR_3	PF13812	321 - 365	5.8e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	349 - 381	2.8e-06	PPR repeat
PPR_2	PF13041	353 - 399	4.1e-12	PPR repeat family
TPR_24	PF23276	361 - 484	2.4e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	376 - 434	3.3e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	387 - 435	4.7e-08	PPR repeat family
PPR_3	PF13812	526 - 575	4.1e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	527 - 574	3.2e-12	PPR repeat family
TPR_24	PF23276	530 - 624	3.8e-11	Fungal tetratrico peptide repeats
PPR	PF01535	532 - 559	1.2e-06	PPR repeat
PPR_long	PF17177	544 - 650	6.4e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	562 - 611	1.4e-07	PPR repeat family
PPR_2	PF13041	598 - 645	1.7e-08	PPR repeat family
PPR_3	PF13812	621 - 681	5.5e-06	Pentatricopeptide repeat domain
TPR_24	PF23276	626 - 727	5.6e-07	Fungal tetratrico peptide repeats
PPR_1	PF12854	629 - 661	1.4e-06	PPR repeat
PPR_2	PF13041	635 - 680	1.4e-07	PPR repeat family
PPR	PF01535	636 - 665	1.1e-06	PPR repeat
PPR_2	PF13041	874 - 920	5e-09	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014465)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73710
fragaria_vesca	FvH4_6g44930
malus_domestica	MD09G1091700.v1.1 MD17G1081600.v1.1
prunus_persica	Prupe.3G233300_v2.0.a1
pyrus_communis	pycom09g01730 pycom17g07990
rosa_chinensis	RchiOBHm_Chr2g0162121
rosa_laevigata	RLG00000021324
rosa_multiflora	Rmu_sc0004020.1_g000031
rosa_roxburghii	Rroxscaffold_2G00088280
rosa_rugosa	Rorug02G0494800
rosa_samantha	Rh2AG561300 Rh2BG574400 Rh2CG544500 Rh2DG584300
rosa_wichuraiana	Rw2G046480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	483, 753, 1064, 1176
AasI	GACNNNNNNGTC	1 cut(s)	1254
AccB1I	GGYRCC	2 cut(s)	1048, 2099
AccB7I	CCANNNNNTGG	2 cut(s)	1873, 2202
AccII	CGCG	2 cut(s)	429, 2921
AccIII	TCCGGA	1 cut(s)	676
AciI	CCGC	6 cut(s)	43, 1118, 1771, 1790, 2319, 2660
AcoI	YGGCCR	1 cut(s)	1837
AcuI	CTGAAG	4 cut(s)	200, 1551, 2184, 2964
AcyI	GRCGYC	1 cut(s)	1000
AfaI	GTAC	4 cut(s)	1865, 2070, 2751, 2843
AfiI	CCNNNNNNNGG	9 cut(s)	628, 1327, 1750, 1873, 2029, 2202, 2358, 2423, 2941
AflIII	ACRYGT	3 cut(s)	1484, 2688, 2919
AjiI	CACGTC	1 cut(s)	900
AjnI	CCWGG	2 cut(s)	1387, 1743
AjuI	GAANNNNNNNTTGG	2 cut(s)	1547, 1579
AleI	CACNNNNGTG	1 cut(s)	640
AloI	GAACNNNNNNTCC	2 cut(s)	1410, 1442
Alw21I	GWGCWC	6 cut(s)	32, 169, 767, 783, 792, 1831
Alw26I	GTCTC	1 cut(s)	2813
Alw44I	GTGCAC	1 cut(s)	763
Ama87I	CYCGRG	1 cut(s)	2579
Aor13HI	TCCGGA	1 cut(s)	676
AoxI	GGCC	4 cut(s)	10, 619, 1682, 1837
ApaLI	GTGCAC	1 cut(s)	763
ApeKI	GCWGC	4 cut(s)	1013, 1220, 1490, 2362
ArsI	GACNNNNNNTTYG	4 cut(s)	334, 366, 1410, 1442
AseI	ATTAAT	2 cut(s)	264, 1410
Asp700I	GAANNNNTTC	1 cut(s)	2690
AspLEI	GCGC	2 cut(s)	429, 2578
AspS9I	GGNCC	3 cut(s)	1318, 1683, 1741
AsuHPI	GGTGA	6 cut(s)	45, 371, 821, 1478, 1742, 2170
AvaI	CYCGRG	1 cut(s)	2579
AvaII	GGWCC	2 cut(s)	1318, 1741
BaeGI	GKGCMC	2 cut(s)	767, 2104
BalI	TGGCCA	1 cut(s)	1839
BanI	GGYRCC	2 cut(s)	1048, 2099
BanII	GRGCYC	5 cut(s)	32, 169, 514, 783, 792
BarI	GAAGNNNNNNTAC	2 cut(s)	2780, 2812
BauI	CACGAG	2 cut(s)	162, 1165
BbsI	GAAGAC	1 cut(s)	2539
Bbv12I	GWGCWC	6 cut(s)	32, 169, 767, 783, 792, 1831
BbvCI	CCTCAGC	2 cut(s)	1536, 2970
BbvI	GCAGC	4 cut(s)	1000, 1207, 1502, 2374
BccI	CCATC	5 cut(s)	1132, 2051, 2149, 2389, 2943
BceAI	ACGGC	1 cut(s)	2834
BcgI	CGANNNNNNTGC	2 cut(s)	2123, 2157
BciT130I	CCWGG	2 cut(s)	1389, 1745
BciVI	GTATCC	1 cut(s)	916
BclI	TGATCA	2 cut(s)	1605, 1920
BcoDI	GTCTC	1 cut(s)	2813
BfmI	CTRYAG	1 cut(s)	1549
BfoI	RGCGCY	1 cut(s)	2579
BfuI	GTATCC	1 cut(s)	916
BglII	AGATCT	1 cut(s)	2089
BisI	GCNGC	7 cut(s)	43, 1014, 1221, 1491, 1790, 2363, 2661
BlsI	GCNGC	7 cut(s)	44, 1015, 1222, 1492, 1791, 2364, 2662
BmcAI	AGTACT	1 cut(s)	2070
Bme1390I	CCNGG	2 cut(s)	1389, 1745
Bme18I	GGWCC	2 cut(s)	1318, 1741
BmeT110I	CYCGRG	1 cut(s)	2579
BmgBI	CACGTC	1 cut(s)	900
BmgT120I	GGNCC	3 cut(s)	1318, 1683, 1741
BmiI	GGNNCC	4 cut(s)	1050, 2101, 2721, 2947
BmrFI	CCNGG	2 cut(s)	1389, 1745
BmrI	ACTGGG	2 cut(s)	512, 777
BmsI	GCATC	9 cut(s)	709, 965, 1000, 1207, 1492, 1840, 2126, 2848, 2860
BmuI	ACTGGG	2 cut(s)	512, 777
BpiI	GAAGAC	1 cut(s)	2539
Bpu10I	CCTNAGC	2 cut(s)	1536, 2970
BpuEI	CTTGAG	1 cut(s)	380
Bsa29I	ATCGAT	1 cut(s)	2133
BsaHI	GRCGYC	1 cut(s)	1000
BsaJI	CCNNGG	2 cut(s)	1834, 2196
BsaWI	WCCGGW	1 cut(s)	676
BsaXI	ACNNNNNCTCC	2 cut(s)	2052, 2082
Bsc4I	CCNNNNNNNGG	9 cut(s)	628, 1327, 1750, 1873, 2029, 2202, 2358, 2423, 2941
Bse1I	ACTGG	3 cut(s)	507, 772, 1945
Bse3DI	GCAATG	1 cut(s)	597
BseAI	TCCGGA	1 cut(s)	676
BseBI	CCWGG	2 cut(s)	1389, 1745
BseCI	ATCGAT	1 cut(s)	2133
BseDI	CCNNGG	2 cut(s)	1834, 2196
BseGI	GGATG	9 cut(s)	529, 685, 724, 1063, 1588, 1759, 2160, 2495, 2713
BseLI	CCNNNNNNNGG	9 cut(s)	628, 1327, 1750, 1873, 2029, 2202, 2358, 2423, 2941
BseMI	GCAATG	1 cut(s)	597
BseMII	CTCAG	9 cut(s)	182, 527, 653, 846, 1527, 1737, 2180, 2943, 2961
BseNI	ACTGG	3 cut(s)	507, 772, 1945
BseSI	GKGCMC	2 cut(s)	767, 2104
BseXI	GCAGC	4 cut(s)	1000, 1207, 1502, 2374
BseYI	CCCAGC	2 cut(s)	411, 2663
BsgI	GTGCAG	2 cut(s)	1509, 1678
Bsh1236I	CGCG	2 cut(s)	429, 2921
BshFI	GGCC	4 cut(s)	12, 621, 1684, 1839
BshNI	GGYRCC	2 cut(s)	1048, 2099
BshVI	ATCGAT	1 cut(s)	2133
BsiHKAI	GWGCWC	6 cut(s)	32, 169, 767, 783, 792, 1831
BsiHKCI	CYCGRG	1 cut(s)	2579
BsiSI	CCGG	1 cut(s)	677
BslFI	GGGAC	4 cut(s)	307, 1690, 2327, 2440
BslI	CCNNNNNNNGG	9 cut(s)	628, 1327, 1750, 1873, 2029, 2202, 2358, 2423, 2941
BsmAI	GTCTC	1 cut(s)	2813
BsmFI	GGGAC	4 cut(s)	307, 1690, 2327, 2440
BsmI	GAATGC	2 cut(s)	1378, 2706
BsnI	GGCC	4 cut(s)	12, 621, 1684, 1839
BsoBI	CYCGRG	1 cut(s)	2579
Bsp1286I	GDGCHC	8 cut(s)	32, 169, 514, 767, 783, 792, 1831, 2104
Bsp13I	TCCGGA	1 cut(s)	676
Bsp1407I	TGTACA	1 cut(s)	2841
BspACI	CCGC	6 cut(s)	43, 1118, 1771, 1790, 2319, 2660
BspANI	GGCC	4 cut(s)	12, 621, 1684, 1839
BspCNI	CTCAG	9 cut(s)	181, 526, 654, 845, 1528, 1736, 2181, 2942, 2962
BspDI	ATCGAT	1 cut(s)	2133
BspEI	TCCGGA	1 cut(s)	676
BspFNI	CGCG	2 cut(s)	429, 2921
BspHI	TCATGA	2 cut(s)	1602, 2694
BspLI	GGNNCC	4 cut(s)	1050, 2101, 2721, 2947
BspT107I	GGYRCC	2 cut(s)	1048, 2099
BsrDI	GCAATG	1 cut(s)	597
BsrGI	TGTACA	1 cut(s)	2841
BsrI	ACTGG	3 cut(s)	507, 772, 1945
BssECI	CCNNGG	2 cut(s)	1834, 2196
BssNI	GRCGYC	1 cut(s)	1000
BssSI	CACGAG	2 cut(s)	162, 1165
BssT1I	CCWWGG	2 cut(s)	1834, 2196
Bst2BI	CACGAG	2 cut(s)	162, 1165
Bst2UI	CCWGG	2 cut(s)	1389, 1745
Bst4CI	ACNGT	5 cut(s)	388, 700, 1232, 2298, 2493
Bst6I	CTCTTC	2 cut(s)	1658, 2811
BstACI	GRCGYC	1 cut(s)	1000
BstAPI	GCANNNNNTGC	2 cut(s)	1826, 2648
BstAUI	TGTACA	1 cut(s)	2841
BstC8I	GCNNGC	8 cut(s)	619, 1822, 1831, 1841, 2258, 2529, 2568, 2804
BstENI	CCTNNNNNAGG	2 cut(s)	626, 2027
BstF5I	GGATG	9 cut(s)	529, 685, 724, 1063, 1588, 1759, 2160, 2495, 2713
BstFNI	CGCG	2 cut(s)	429, 2921
BstH2I	RGCGCY	1 cut(s)	2579
BstHHI	GCGC	2 cut(s)	429, 2578
BstMAI	GTCTC	1 cut(s)	2813
BstMWI	GCNNNNNNNGC	7 cut(s)	787, 1808, 1817, 1826, 1830, 2648, 2657
BstNI	CCWGG	2 cut(s)	1389, 1745
BstNSI	RCATGY	6 cut(s)	1488, 1824, 2260, 2570, 2651, 2692
BstSCI	CCNGG	2 cut(s)	1387, 1743
BstSFI	CTRYAG	1 cut(s)	1549
BstSLI	GKGCMC	2 cut(s)	767, 2104
BstUI	CGCG	2 cut(s)	429, 2921
BstV1I	GCAGC	4 cut(s)	1000, 1207, 1502, 2374
BstV2I	GAAGAC	1 cut(s)	2539
BstX2I	RGATCY	1 cut(s)	2089
BstYI	RGATCY	1 cut(s)	2089
Bsu15I	ATCGAT	1 cut(s)	2133
BsuI	GTATCC	1 cut(s)	916
BsuRI	GGCC	4 cut(s)	12, 621, 1684, 1839
BsuTUI	ATCGAT	1 cut(s)	2133
BtrI	CACGTC	1 cut(s)	900
BtsCI	GGATG	9 cut(s)	529, 685, 724, 1063, 1588, 1759, 2160, 2495, 2713
BtsIMutI	CAGTG	3 cut(s)	224, 765, 864
Cac8I	GCNNGC	8 cut(s)	619, 1822, 1831, 1841, 2258, 2529, 2568, 2804
CciI	TCATGA	2 cut(s)	1602, 2694
CfoI	GCGC	2 cut(s)	429, 2578
Cfr13I	GGNCC	3 cut(s)	1318, 1683, 1741
ClaI	ATCGAT	1 cut(s)	2133
CseI	GACGC	1 cut(s)	989
Csp6I	GTAC	4 cut(s)	1864, 2069, 2750, 2842
CspCI	CAANNNNNGTGG	2 cut(s)	865, 900
CviQI	GTAC	4 cut(s)	1864, 2069, 2750, 2842
DraI	TTTAAA	1 cut(s)	2434
DrdI	GACNNNNNNGTC	1 cut(s)	1254
DseDI	GACNNNNNNGTC	1 cut(s)	1254
EaeI	YGGCCR	1 cut(s)	1837
Eam1104I	CTCTTC	2 cut(s)	1658, 2811
EarI	CTCTTC	2 cut(s)	1658, 2811
Ecl136II	GAGCTC	4 cut(s)	30, 167, 781, 790
Eco130I	CCWWGG	2 cut(s)	1834, 2196
Eco147I	AGGCCT	1 cut(s)	621
Eco24I	GRGCYC	5 cut(s)	32, 169, 514, 783, 792
Eco47I	GGWCC	2 cut(s)	1318, 1741
Eco53kI	GAGCTC	4 cut(s)	30, 167, 781, 790
Eco57I	CTGAAG	4 cut(s)	200, 1551, 2184, 2964
Eco88I	CYCGRG	1 cut(s)	2579
EcoICRI	GAGCTC	4 cut(s)	30, 167, 781, 790
EcoNI	CCTNNNNNAGG	2 cut(s)	626, 2027
EcoRI	GAATTC	2 cut(s)	157, 891
EcoRII	CCWGG	2 cut(s)	1387, 1743
EcoT14I	CCWWGG	2 cut(s)	1834, 2196
EcoT22I	ATGCAT	2 cut(s)	1507, 2568
EcoT38I	GRGCYC	5 cut(s)	32, 169, 514, 783, 792
ErhI	CCWWGG	2 cut(s)	1834, 2196
FaqI	GGGAC	4 cut(s)	307, 1690, 2327, 2440
FauI	CCCGC	1 cut(s)	1764
FauNDI	CATATG	4 cut(s)	586, 1507, 2035, 2221
FbaI	TGATCA	2 cut(s)	1605, 1920
Fnu4HI	GCNGC	7 cut(s)	43, 1014, 1221, 1491, 1790, 2363, 2661
FokI	GGATG	9 cut(s)	536, 692, 731, 1070, 1595, 1766, 2167, 2482, 2720
FriOI	GRGCYC	5 cut(s)	32, 169, 514, 783, 792
Fsp4HI	GCNGC	7 cut(s)	43, 1014, 1221, 1491, 1790, 2363, 2661
GlaI	GCGC	2 cut(s)	428, 2577
GluI	GCNGC	7 cut(s)	43, 1014, 1221, 1491, 1790, 2363, 2661
GsaI	CCCAGC	2 cut(s)	415, 2667
HaeII	RGCGCY	1 cut(s)	2579
HaeIII	GGCC	4 cut(s)	12, 621, 1684, 1839
HapII	CCGG	1 cut(s)	677
HgaI	GACGC	1 cut(s)	989
HhaI	GCGC	2 cut(s)	429, 2578
Hin1I	GRCGYC	1 cut(s)	1000
Hin6I	GCGC	2 cut(s)	427, 2576
HinP1I	GCGC	2 cut(s)	427, 2576
HindIII	AAGCTT	5 cut(s)	62, 212, 1611, 1628, 2615
HpaII	CCGG	1 cut(s)	677
HphI	GGTGA	6 cut(s)	45, 371, 821, 1478, 1742, 2170
Hpy166II	GTNNAC	5 cut(s)	765, 864, 1741, 1864, 2047
Hpy8I	GTNNAC	5 cut(s)	765, 864, 1741, 1864, 2047
HpyCH4III	ACNGT	5 cut(s)	388, 700, 1232, 2298, 2493
HpyCH4IV	ACGT	6 cut(s)	899, 1147, 1599, 2727, 2735, 2752
HpyF10VI	GCNNNNNNNGC	7 cut(s)	787, 1808, 1817, 1826, 1830, 2648, 2657
HpySE526I	ACGT	6 cut(s)	899, 1147, 1599, 2727, 2735, 2752
Hsp92I	GRCGYC	1 cut(s)	1000
HspAI	GCGC	2 cut(s)	427, 2576
Kpn2I	TCCGGA	1 cut(s)	676
Ksp22I	TGATCA	2 cut(s)	1605, 1920
LmnI	GCTCC	3 cut(s)	98, 408, 2060
Lsp1109I	GCAGC	4 cut(s)	1000, 1207, 1502, 2374
LweI	GCATC	9 cut(s)	709, 965, 1000, 1207, 1492, 1840, 2126, 2848, 2860
MaeII	ACGT	6 cut(s)	899, 1147, 1599, 2727, 2735, 2752
MaeIII	GTNAC	8 cut(s)	447, 685, 809, 2158, 2398, 2479, 2487, 2731
MflI	RGATCY	1 cut(s)	2089
MhlI	GDGCHC	8 cut(s)	32, 169, 514, 767, 783, 792, 1831, 2104
MlsI	TGGCCA	1 cut(s)	1839
MluI	ACGCGT	1 cut(s)	2919
MluNI	TGGCCA	1 cut(s)	1839
MlyI	GAGTC	6 cut(s)	329, 806, 824, 2042, 2591, 2972
MmeI	TCCRAC	2 cut(s)	521, 1092
Mox20I	TGGCCA	1 cut(s)	1839
Mph1103I	ATGCAT	2 cut(s)	1507, 2568
MroI	TCCGGA	1 cut(s)	676
MroXI	GAANNNNTTC	1 cut(s)	2690
MscI	TGGCCA	1 cut(s)	1839
MslI	CAYNNNNRTG	7 cut(s)	483, 640, 1400, 1715, 2652, 2693, 2699
Msp20I	TGGCCA	1 cut(s)	1839
MspA1I	CMGCKG	1 cut(s)	2806
MspI	CCGG	1 cut(s)	677
MspR9I	CCNGG	2 cut(s)	1389, 1745
Mva1269I	GAATGC	2 cut(s)	1378, 2706
MvaI	CCWGG	2 cut(s)	1389, 1745
MvnI	CGCG	2 cut(s)	429, 2921
MwoI	GCNNNNNNNGC	7 cut(s)	787, 1808, 1817, 1826, 1830, 2648, 2657
NdeI	CATATG	4 cut(s)	586, 1507, 2035, 2221
NlaIV	GGNNCC	4 cut(s)	1050, 2101, 2721, 2947
NmuCI	GTSAC	3 cut(s)	447, 809, 2158
NsiI	ATGCAT	2 cut(s)	1507, 2568
NspI	RCATGY	6 cut(s)	1488, 1824, 2260, 2570, 2651, 2692
OliI	CACNNNNGTG	1 cut(s)	640
PaeI	GCATGC	3 cut(s)	1824, 2260, 2570
PaeR7I	CTCGAG	1 cut(s)	2579
PagI	TCATGA	2 cut(s)	1602, 2694
PceI	AGGCCT	1 cut(s)	621
PciI	ACATGT	2 cut(s)	1484, 2688
PcsI	WCGNNNNNNNCGW	2 cut(s)	789, 946
PctI	GAATGC	2 cut(s)	1378, 2706
PdmI	GAANNNNTTC	1 cut(s)	2690
PfeI	GAWTC	5 cut(s)	370, 800, 1339, 1985, 2621
PflMI	CCANNNNNTGG	2 cut(s)	1873, 2202
PkrI	GCNGC	7 cut(s)	44, 1015, 1222, 1492, 1791, 2364, 2662
PleI	GAGTC	6 cut(s)	328, 806, 824, 2042, 2590, 2971
PpsI	GAGTC	6 cut(s)	328, 806, 824, 2042, 2590, 2971
PscI	ACATGT	2 cut(s)	1484, 2688
PshBI	ATTAAT	2 cut(s)	264, 1410
PsiI	TTATAA	4 cut(s)	483, 753, 1064, 1176
Psp124BI	GAGCTC	4 cut(s)	32, 169, 783, 792
Psp6I	CCWGG	2 cut(s)	1387, 1743
PspFI	CCCAGC	2 cut(s)	411, 2663
PspGI	CCWGG	2 cut(s)	1387, 1743
PspN4I	GGNNCC	4 cut(s)	1050, 2101, 2721, 2947
PspPI	GGNCC	3 cut(s)	1318, 1683, 1741
PspXI	VCTCGAGB	1 cut(s)	2579
PsuI	RGATCY	1 cut(s)	2089
PvuII	CAGCTG	1 cut(s)	2806
RsaI	GTAC	4 cut(s)	1865, 2070, 2751, 2843
RsaNI	GTAC	4 cut(s)	1864, 2069, 2750, 2842
RseI	CAYNNNNRTG	7 cut(s)	483, 640, 1400, 1715, 2652, 2693, 2699
SacI	GAGCTC	4 cut(s)	32, 169, 783, 792
SatI	GCNGC	7 cut(s)	43, 1014, 1221, 1491, 1790, 2363, 2661
Sau96I	GGNCC	3 cut(s)	1318, 1683, 1741
ScaI	AGTACT	1 cut(s)	2070
SchI	GAGTC	6 cut(s)	329, 806, 824, 2042, 2591, 2972
ScrFI	CCNGG	2 cut(s)	1389, 1745
SduI	GDGCHC	8 cut(s)	32, 169, 514, 767, 783, 792, 1831, 2104
SfaNI	GCATC	9 cut(s)	709, 965, 1000, 1207, 1492, 1840, 2126, 2848, 2860
SfcI	CTRYAG	1 cut(s)	1549
Sfr274I	CTCGAG	1 cut(s)	2579
SinI	GGWCC	2 cut(s)	1318, 1741
SlaI	CTCGAG	1 cut(s)	2579
SmiMI	CAYNNNNRTG	7 cut(s)	483, 640, 1400, 1715, 2652, 2693, 2699
SmlI	CTYRAG	2 cut(s)	395, 2579
SmoI	CTYRAG	2 cut(s)	395, 2579
SphI	GCATGC	3 cut(s)	1824, 2260, 2570
SseBI	AGGCCT	1 cut(s)	621
SsiI	CCGC	6 cut(s)	43, 1118, 1771, 1790, 2319, 2660
SspI	AATATT	1 cut(s)	2439
SstI	GAGCTC	4 cut(s)	32, 169, 783, 792
StuI	AGGCCT	1 cut(s)	621
StyD4I	CCNGG	2 cut(s)	1387, 1743
StyI	CCWWGG	2 cut(s)	1834, 2196
TaaI	ACNGT	5 cut(s)	388, 700, 1232, 2298, 2493
TaiI	ACGT	6 cut(s)	902, 1150, 1602, 2730, 2738, 2755
TatI	WGTACW	2 cut(s)	2068, 2841
TauI	GCSGC	3 cut(s)	45, 1792, 2663
TfiI	GAWTC	5 cut(s)	370, 800, 1339, 1985, 2621
TscAI	CASTG	3 cut(s)	224, 772, 871
TseFI	GTSAC	3 cut(s)	447, 809, 2158
TseI	GCWGC	4 cut(s)	1013, 1220, 1490, 2362
Tsp45I	GTSAC	3 cut(s)	447, 809, 2158
TspGWI	ACGGA	1 cut(s)	1941
TspRI	CASTG	3 cut(s)	224, 772, 871
Van91I	CCANNNNNTGG	2 cut(s)	1873, 2202
VneI	GTGCAC	1 cut(s)	763
VpaK11BI	GGWCC	2 cut(s)	1318, 1741
VspI	ATTAAT	2 cut(s)	264, 1410
XagI	CCTNNNNNAGG	2 cut(s)	626, 2027
XbaI	TCTAGA	2 cut(s)	316, 2092
XceI	RCATGY	6 cut(s)	1488, 1824, 2260, 2570, 2651, 2692
XhoI	CTCGAG	1 cut(s)	2579
XmnI	GAANNNNTTC	1 cut(s)	2690
ZrmI	AGTACT	1 cut(s)	2070
Zsp2I	ATGCAT	2 cut(s)	1507, 2568

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.