Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0014465

Physical Location & Seq

Genomic Coordinates Forward (+)

1327303 .. 1333890

Gene Structure 6588 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g01730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3837 bp

ATGCCATACAACGAGTGCAAACGAAATCCCAATCCATCTACAGAGGATCCAGTATGTTGCGTTCACAACATATTTGGGTTTGATTGTGTGTCTGCTGTGGAATTTTCTGGCAGTTACCGTAGCCTGGATCAAGGGAGAAGTGTAATAATTTGGCTTCTGGCTATAATCTACTTCCTAGCAAGTGTTCCTGTAGCCTATTTCGTCTGGTATCGCCCTCTTTATCGTGCTATGAGGACTGATAGTGCTCTGAGTTTTGTTGGGTTTTTCTTGTCTTATCTGCTCCATATTTTGTTTTGTATTTACGCTGCAATTCACATCCGGTCGATAAAATTTAACTCAGAAGTGTTTATACCTCACAAAAGCACACTAAGATTAAGCAGGTATCAAATAATATGCGGTCGGTTTTATCCCCGAGGCTCATCTGGTTCCTACATGAGAGGACAAACTTCTGAAAAAACCGGCGAGCCCCCGCCGCCGAAGAGTCCGAGTAGAAAGCAGGCAATGATGCTCCACAGCCAGAGTTACAGCCCTATGGAATTAGGCCAAGAAAGTTTCCAGGGCTTAATTCACAGCCGCACTTTTTCACCAAGTAAGCTTCGAAACCCCCATCAATATCCTTTCAGAGCTAGGGCTTTTCTAGCATTTAGCTTACCCAATTCTCCGAATGTCGAAAACATTGGGAATTCACGGGCTCAGAAGCAGAATTTGAGAGGTTCTAGGGTTTTGGTAGGGTTTAAGCTTCAGTGTCATTCTAAATCGTTGGTTTTGCCCACGAAAGTTTCTTCTGTTAATGGTAAGAAGAAGGGGTATGGAGGCATGTTGCCTTCGATTTTACGGTCTTTAGAGTCCGAAAACGATGTTGAAAAGACCCTTAATTCGTTAGGTGAAAATTTGAATCCGAAAGAACAGACTGTGATTCTCAAGGAACAAAAGAGCTGGGAAAGGGTTTTGCGCGTTTTTGACTGGTTCAAGTCACAGAAAGAGTATGTTCCCAATGTAATACATTATAATGTGGTGCTTCGGAAACTGGGTAGGGCTCAGAAGTGGGATGAATTGAGGCTGTGTTGGATTGAAATGGCGAAAATGGGTGTTCTGCCAACGAACAACACATATGCAATGCTCGTTGATGTGTATGGGAAAGCAGGCCTTGTGAAGGAAGCGCTTTTGTGGATTAAGCACATGAAACTGAGGGGAATGTTTCCGGATGATGTTACTATGAATACAGTTGTTCGGGCATTGAAGGATGCAGGAGAGTTTGACAGGGCAGACAAGTTTTATAAGGATTGGTGCACTGGAAAGGTTGAGCTCGATGAGCTTGATTTGGATTCTATGGGTGACTCTGTGAATGGTTCTGACTCAGAGCCTATTAGTTTTAAGCACTTCTTGTCCACTGAACTTTTCAAGACAGGTGGGAGAATTCCCACGTCAAAGATTACGACCTCATTGGATACACAGAACATTGGTCGAAAACCACGACAGGCATCTACATATAATGCTTTGATTGATTTGTACGGGAAGGCAGGGCGTCTTGATGATGCTGCTAATGTGTTTGGAGAAATGATGAAGTCTGGGGTGCCAATGGATGTTATAACTTTCAACACTATGATCTTTACTTGTGGAAGTCATGGTCATTTGTTGGAAGCGGAAACTTTGCTTGGTAAGATGGAAGAAAGGGGAATTTCTCCTGACACAAGAACTTATAACATTTTTCTATCTTTGTATGCTGATGTGGGGAACATTGATGCTGCTCTGAACTGTTATATGAAGATCAGAGAGTTGGGTCTTTCCCCAGACATCGTATCTCACAGGACTATTCTTCATGTGTTATGTGAGAGAAACATGGTCCGAGAAGTGGAGACTGTGATTCAGGATATGGAGAAATCTGGTGTGCGCGTCGATGAGCATTCTGTTCCTGGTGTCATCAAGATGTATATTAACGAAGGACAGCTTGTTCGGGCAAAGTTGTTTTACGAGAAATGTCAGTTGATTGGTGAGCTGTCATCAAAGACATGTGCAGCAATTATAGATGCATATGCTGAAAAGAGATTTTGGACTGAAGCTGAGGCGGTATTCTATAGGAAGAAAGACTTGGTGAGACAGAAGAAGGATGTTGTGGAGTATAACGTCATGATCAAAGCTTATGGCAAGGCAAAGCTTTATGATAAAGCTTTTTCTCTCTTCAAGGGCATGAGAAATCATGGGACTTGGCCCGATAACTGCACATATAATTCTCTCATTCAGATGTTCTCAGGAGGTGATTTAGTGGACCAGGCAAGGGATGTTTTAACAGAAATGCGGGAAATGGGTTTTAAGCCACACTCTCTAGCCTTCTCTGCTCTAATTGCATGCTGCGCTCGCCTTGGCCAGCTTTCTGATGCAGTTGATGTGTACCAAGACTTGGTAAATTCAGGGGTCAAACCAAATGAATTTGTATATGGTTCTTTGATCAACGGATTTGTAGAAACTGGCAGAGTTGAGGAAGCATTAAAGTATTTTCGGCACATGGAAGAATCTGGGATTTCTGCAAATCAAATAATTCTTACTTCCCTCATAAAGGCATATGGTAAGGTGGACTCCCTTGATGGAGCGAAAGTACTCTATGAGAAGTTGAAAGATCTAGAGGGTGCCCGAGATATTGTTGCATCTAACAGCATGATCGATCTCTATGCAGATCGTGGGATGGTGACTGAAGCCGAATTGGTTTTTGAAAAACTGAGAGCCAAGGGTTGGGCAAATGAGATAACATATGCGACCATGATATATCTTTACAAGAGTGTGGGCATGCTTGATGAAGCCATTGATATAGCAGAGGAAATGAAACTGTCAGGTCTAGTGAGGGACTGCGGTTCATTTAACAAGGTAATGTCATGTTATGCCATTAACGGGCAGCTACGGGAATGTGGCGAATTATTGCATGAGATGATAACTTGGAAACTCTTGCCAGACATCGGGACTTTTAAAGTATTATTCACGATATTGAAAAAAGGTGTTTCAGTTGAAGCCATAACGCAGTTAGAGTCATCCTACCATGAAGGGAAACCTTACTCTAGGCAAGCGATCATCACCTCTGTCTTCTCTATGGTGGGTATGCATGCTTTGGCGCTCGAATCCTGTGAAAATTTCACAAAAGCTGATGTAAAGCTTGATTCTTTTCTCTATAATGTTGCTCTGAACATGTTCATGAGAATGCAAGATGAAAACTTGGAACCAGACGTTGTAACGTATATTAACTTAGTACGTTGCTATGGAAAAGCTGGTATGCTTGAAGGTGTGAAGCGAATATACAGCCAGCTGAAATATGAAGAGACACAGCCAAATCATTCCTTGTACAAAGCCGTTCTTGATGCCTATACAGATGCCAACAGGCATGACCTTGCCAAATTGGTTAGCCAAGAGATGAGATACGCCTTCTACTCAGACCAGCGGACGGGTTCTGAAACTAAAGACGAGTCTGATGAGAAACTCCATGTGGTGTTACACTGTACAGAAACACATTTGTTCATGCATCTAATACCGTATCGTATAAATGTACCAGGTTCGACGGAACATGTACTGTATTTCCCATTTGTTAAATTAAATATCAATAATATTCCAACACTAAGCGCAAATTCTCTTATCCGATTTAGTAGGGAAAAATGGGAACCATTCAAATTTCACGGAATGAAATTTGATTCTACTACGAATTTCCACATCTCCGACATGGAGATTAGAACCCAATTTACCGTTGGATTCAATCCAGCTCTTAGCCGGCATCTTTTTAACCCCTCTCTGTTGCATTATCCTCCCAATCTCCGAGGCGTCATTCCATCGGCCTACTGTTCTATACACATTAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

1279

Amino Acids

145.12

Weight (kDa)

8.33

Isoelectric Point (pI)

35.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SCAMP	PF04144	40 - 107	5.9e-16	SCAMP family
PPR_2	PF13041	331 - 378	6.8e-06	PPR repeat family
PPR_2	PF13041	495 - 539	3.2e-12	PPR repeat family
PPR_long	PF17177	495 - 658	1.9e-07	Pentacotripeptide-repeat region of PRORP
PPR	PF01535	495 - 525	5.6e-08	PPR repeat
PPR_3	PF13812	496 - 540	7.7e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	496 - 520	1.5e-06	PPR repeat
PPR_2	PF13041	528 - 574	6.8e-11	PPR repeat family
TPR_24	PF23276	536 - 659	5.3e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	551 - 609	1.3e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	566 - 610	7e-07	PPR repeat family
PPR_3	PF13812	701 - 750	5.5e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	702 - 749	4.3e-12	PPR repeat family
TPR_24	PF23276	704 - 799	5e-11	Fungal tetratrico peptide repeats
PPR	PF01535	707 - 734	1.6e-06	PPR repeat
PPR_long	PF17177	718 - 825	8.7e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	737 - 786	1.8e-07	PPR repeat family
PPR_2	PF13041	773 - 820	2.2e-08	PPR repeat family
PPR_3	PF13812	796 - 856	8.8e-06	Pentatricopeptide repeat domain
TPR_24	PF23276	801 - 902	4.4e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	804 - 836	1.9e-06	PPR repeat
PPR	PF01535	811 - 840	1.4e-06	PPR repeat
PPR_2	PF13041	811 - 855	2e-06	PPR repeat family
PPR_2	PF13041	950 - 992	1e-05	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014465)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73710
fragaria_vesca	FvH4_6g44930
malus_domestica	MD09G1091700.v1.1 MD17G1081600.v1.1
prunus_persica	Prupe.3G233300_v2.0.a1
pyrus_communis	pycom09g01730 pycom17g07990
rosa_chinensis	RchiOBHm_Chr2g0162121
rosa_laevigata	RLG00000021324
rosa_multiflora	Rmu_sc0004020.1_g000031
rosa_roxburghii	Rroxscaffold_2G00088280
rosa_rugosa	Rorug02G0494800
rosa_samantha	Rh2AG561300 Rh2BG574400 Rh2CG544500 Rh2DG584300
rosa_wichuraiana	Rw2G046480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	1008, 1278, 1589, 1701
Acc36I	ACCTGC	1 cut(s)	369
AccB1I	GGYRCC	2 cut(s)	1573, 2624
AccB7I	CCANNNNNTGG	2 cut(s)	2398, 2727
AccII	CGCG	2 cut(s)	954, 1893
AccIII	TCCGGA	1 cut(s)	1201
AciI	CCGC	9 cut(s)	396, 470, 473, 574, 1643, 2066, 2296, 2844, 3424
AclWI	GGATC	3 cut(s)	41, 54, 135
AcoI	YGGCCR	1 cut(s)	2362
AcuI	CTGAAG	3 cut(s)	725, 2076, 2709
AcyI	GRCGYC	2 cut(s)	1525, 3798
AfaI	GTAC	8 cut(s)	1511, 2390, 2595, 3237, 3329, 3484, 3531, 3552
AfeI	AGCGCT	1 cut(s)	1161
AflIII	ACRYGT	3 cut(s)	2009, 3174, 3547
AjiI	CACGTC	1 cut(s)	1425
AjnI	CCWGG	5 cut(s)	123, 555, 1912, 2268, 3532
AjuI	GAANNNNNNNTTGG	4 cut(s)	1638, 1670, 2072, 2104
AloI	GAACNNNNNNTCC	2 cut(s)	1935, 1967
Alw21I	GWGCWC	3 cut(s)	247, 1292, 1308
Alw26I	GTCTC	3 cut(s)	1850, 2089, 3299
Alw44I	GTGCAC	1 cut(s)	1288
AlwI	GGATC	3 cut(s)	41, 54, 135
Ama87I	CYCGRG	2 cut(s)	411, 2628
Aor13HI	TCCGGA	1 cut(s)	1201
Aor51HI	AGCGCT	1 cut(s)	1161
AoxI	GGCC	5 cut(s)	541, 1144, 2207, 2362, 3810
ApaLI	GTGCAC	1 cut(s)	1288
ApeKI	GCWGC	6 cut(s)	305, 1538, 1745, 2015, 2349, 2887
ArsI	GACNNNNNNTTYG	4 cut(s)	859, 891, 1935, 1967
Asp700I	GAANNNNTTC	1 cut(s)	3176
AspLEI	GCGC	6 cut(s)	954, 1162, 1893, 2354, 3103, 3605
AspS9I	GGNCC	3 cut(s)	1843, 2208, 2266
AsuHPI	GGTGA	8 cut(s)	576, 896, 1346, 2003, 2104, 2267, 2695, 3055
AsuII	TTCGAA	1 cut(s)	598
AvaI	CYCGRG	2 cut(s)	411, 2628
AvaII	GGWCC	2 cut(s)	1843, 2266
BaeGI	GKGCMC	2 cut(s)	1292, 2629
BalI	TGGCCA	1 cut(s)	2364
BamHI	GGATCC	1 cut(s)	46
BanI	GGYRCC	2 cut(s)	1573, 2624
BanII	GRGCYC	4 cut(s)	468, 694, 1039, 1308
BarI	GAAGNNNNNNTAC	4 cut(s)	333, 365, 3266, 3298
BbsI	GAAGAC	1 cut(s)	3064
Bbv12I	GWGCWC	3 cut(s)	247, 1292, 1308
BbvCI	CCTCAGC	1 cut(s)	2061
BbvI	GCAGC	6 cut(s)	292, 1525, 1732, 2027, 2336, 2899
BccI	CCATC	6 cut(s)	43, 615, 1657, 2576, 2674, 3814
BceAI	ACGGC	1 cut(s)	3320
BcgI	CGANNNNNNTGC	2 cut(s)	2648, 2682
BciT130I	CCWGG	5 cut(s)	125, 557, 1914, 2270, 3534
BciVI	GTATCC	1 cut(s)	1441
BclI	TGATCA	2 cut(s)	2130, 2445
BcoDI	GTCTC	3 cut(s)	1850, 2089, 3299
BfaI	CTAG	9 cut(s)	176, 627, 638, 717, 2324, 2618, 2831, 3048, 3835
BfmI	CTRYAG	3 cut(s)	39, 189, 2074
BfoI	RGCGCY	2 cut(s)	1163, 3104
BfuAI	ACCTGC	1 cut(s)	369
BfuI	GTATCC	1 cut(s)	1441
BglII	AGATCT	1 cut(s)	2614
BisI	GCNGC	8 cut(s)	306, 473, 574, 1539, 1746, 2016, 2350, 2888
BlsI	GCNGC	8 cut(s)	307, 474, 575, 1540, 1747, 2017, 2351, 2889
BmcAI	AGTACT	1 cut(s)	2595
Bme1390I	CCNGG	5 cut(s)	125, 557, 1914, 2270, 3534
Bme18I	GGWCC	2 cut(s)	1843, 2266
BmeT110I	CYCGRG	2 cut(s)	411, 2628
BmgBI	CACGTC	1 cut(s)	1425
BmgT120I	GGNCC	3 cut(s)	1843, 2208, 2266
BmiI	GGNNCC	6 cut(s)	48, 427, 1575, 2626, 3207, 3642
BmrFI	CCNGG	5 cut(s)	125, 557, 1914, 2270, 3534
BmrI	ACTGGG	1 cut(s)	1037
BmuI	ACTGGG	1 cut(s)	1037
BpiI	GAAGAC	1 cut(s)	3064
Bpu10I	CCTNAGC	1 cut(s)	2061
Bpu14I	TTCGAA	1 cut(s)	598
BpuEI	CTTGAG	1 cut(s)	905
Bsa29I	ATCGAT	1 cut(s)	2658
BsaHI	GRCGYC	2 cut(s)	1525, 3798
BsaJI	CCNNGG	5 cut(s)	412, 556, 2359, 2721, 3793
BsaWI	WCCGGW	2 cut(s)	318, 1201
BsaXI	ACNNNNNCTCC	2 cut(s)	2577, 2607
Bse118I	RCCGGY	2 cut(s)	458, 3747
Bse1I	ACTGG	5 cut(s)	50, 968, 1032, 1297, 2470
Bse3DI	GCAATG	2 cut(s)	507, 1122
BseAI	TCCGGA	1 cut(s)	1201
BseBI	CCWGG	5 cut(s)	125, 557, 1914, 2270, 3534
BseCI	ATCGAT	1 cut(s)	2658
BseDI	CCNNGG	5 cut(s)	412, 556, 2359, 2721, 3793
BseGI	GGATG	9 cut(s)	315, 1054, 1210, 1249, 1588, 2113, 2284, 2685, 3020
BseMI	GCAATG	2 cut(s)	507, 1122
BseNI	ACTGG	5 cut(s)	50, 968, 1032, 1297, 2470
BseSI	GKGCMC	2 cut(s)	1292, 2629
BseXI	GCAGC	6 cut(s)	292, 1525, 1732, 2027, 2336, 2899
BseYI	CCCAGC	1 cut(s)	936
BsgI	GTGCAG	2 cut(s)	2034, 2203
Bsh1236I	CGCG	2 cut(s)	954, 1893
Bsh1285I	CGRYCG	2 cut(s)	323, 400
BshFI	GGCC	5 cut(s)	543, 1146, 2209, 2364, 3812
BshNI	GGYRCC	2 cut(s)	1573, 2624
BshVI	ATCGAT	1 cut(s)	2658
BsiEI	CGRYCG	2 cut(s)	323, 400
BsiHKAI	GWGCWC	3 cut(s)	247, 1292, 1308
BsiHKCI	CYCGRG	2 cut(s)	411, 2628
BsiSI	CCGG	4 cut(s)	319, 459, 1202, 3748
BslFI	GGGAC	3 cut(s)	2215, 2852, 2965
BsmAI	GTCTC	3 cut(s)	1850, 2089, 3299
BsmFI	GGGAC	3 cut(s)	2215, 2852, 2965
BsmI	GAATGC	2 cut(s)	1903, 3192
BsnI	GGCC	5 cut(s)	543, 1146, 2209, 2364, 3812
BsoBI	CYCGRG	2 cut(s)	411, 2628
Bsp119I	TTCGAA	1 cut(s)	598
Bsp1286I	GDGCHC	7 cut(s)	247, 468, 694, 1039, 1292, 1308, 2629
Bsp13I	TCCGGA	1 cut(s)	1201
Bsp1407I	TGTACA	2 cut(s)	3327, 3482
BspACI	CCGC	9 cut(s)	396, 470, 473, 574, 1643, 2066, 2296, 2844, 3424
BspANI	GGCC	5 cut(s)	543, 1146, 2209, 2364, 3812
BspDI	ATCGAT	1 cut(s)	2658
BspEI	TCCGGA	1 cut(s)	1201
BspFNI	CGCG	2 cut(s)	954, 1893
BspHI	TCATGA	2 cut(s)	2127, 3180
BspLI	GGNNCC	6 cut(s)	48, 427, 1575, 2626, 3207, 3642
BspMI	ACCTGC	1 cut(s)	369
BspPI	GGATC	3 cut(s)	41, 54, 135
BspT104I	TTCGAA	1 cut(s)	598
BspT107I	GGYRCC	2 cut(s)	1573, 2624
BsrDI	GCAATG	2 cut(s)	507, 1122
BsrFI	RCCGGY	2 cut(s)	458, 3747
BsrGI	TGTACA	2 cut(s)	3327, 3482
BsrI	ACTGG	5 cut(s)	50, 968, 1032, 1297, 2470
BssAI	RCCGGY	2 cut(s)	458, 3747
BssECI	CCNNGG	5 cut(s)	412, 556, 2359, 2721, 3793
BssNI	GRCGYC	2 cut(s)	1525, 3798
BssT1I	CCWWGG	2 cut(s)	2359, 2721
Bst2UI	CCWGG	5 cut(s)	125, 557, 1914, 2270, 3534
Bst6I	CTCTTC	3 cut(s)	473, 2183, 3297
BstACI	GRCGYC	2 cut(s)	1525, 3798
BstAUI	TGTACA	2 cut(s)	3327, 3482
BstBI	TTCGAA	1 cut(s)	598
BstENI	CCTNNNNNAGG	2 cut(s)	1151, 2552
BstF5I	GGATG	9 cut(s)	315, 1054, 1210, 1249, 1588, 2113, 2284, 2685, 3020
BstFNI	CGCG	2 cut(s)	954, 1893
BstH2I	RGCGCY	2 cut(s)	1163, 3104
BstHHI	GCGC	6 cut(s)	954, 1162, 1893, 2354, 3103, 3605
BstMAI	GTCTC	3 cut(s)	1850, 2089, 3299
BstMCI	CGRYCG	2 cut(s)	323, 400
BstMWI	GCNNNNNNNGC	7 cut(s)	472, 638, 1312, 2333, 2342, 2351, 2355
BstNI	CCWGG	5 cut(s)	125, 557, 1914, 2270, 3534
BstNSI	RCATGY	7 cut(s)	820, 2013, 2349, 2785, 3095, 3178, 3551
BstSCI	CCNGG	5 cut(s)	123, 555, 1912, 2268, 3532
BstSFI	CTRYAG	3 cut(s)	39, 189, 2074
BstSLI	GKGCMC	2 cut(s)	1292, 2629
BstUI	CGCG	2 cut(s)	954, 1893
BstV1I	GCAGC	6 cut(s)	292, 1525, 1732, 2027, 2336, 2899
BstV2I	GAAGAC	1 cut(s)	3064
BstX2I	RGATCY	2 cut(s)	46, 2614
BstYI	RGATCY	2 cut(s)	46, 2614
Bsu15I	ATCGAT	1 cut(s)	2658
BsuI	GTATCC	1 cut(s)	1441
BsuRI	GGCC	5 cut(s)	543, 1146, 2209, 2364, 3812
BsuTUI	ATCGAT	1 cut(s)	2658
BtrI	CACGTC	1 cut(s)	1425
BtsCI	GGATG	9 cut(s)	315, 1054, 1210, 1249, 1588, 2113, 2284, 2685, 3020
BtsIMutI	CAGTG	4 cut(s)	749, 1290, 1389, 3478
BveI	ACCTGC	1 cut(s)	369
CciI	TCATGA	2 cut(s)	2127, 3180
CfoI	GCGC	6 cut(s)	954, 1162, 1893, 2354, 3103, 3605
Cfr10I	RCCGGY	2 cut(s)	458, 3747
Cfr13I	GGNCC	3 cut(s)	1843, 2208, 2266
ClaI	ATCGAT	1 cut(s)	2658
CseI	GACGC	3 cut(s)	1514, 1882, 3787
CsiI	ACCWGGT	1 cut(s)	3532
Csp6I	GTAC	8 cut(s)	1510, 2389, 2594, 3236, 3328, 3483, 3530, 3551
CspCI	CAANNNNNGTGG	2 cut(s)	1390, 1425
CviQI	GTAC	8 cut(s)	1510, 2389, 2594, 3236, 3328, 3483, 3530, 3551
DraI	TTTAAA	1 cut(s)	2959
EaeI	YGGCCR	1 cut(s)	2362
Eam1104I	CTCTTC	3 cut(s)	473, 2183, 3297
EarI	CTCTTC	3 cut(s)	473, 2183, 3297
Ecl136II	GAGCTC	1 cut(s)	1306
Eco130I	CCWWGG	2 cut(s)	2359, 2721
Eco147I	AGGCCT	1 cut(s)	1146
Eco24I	GRGCYC	4 cut(s)	468, 694, 1039, 1308
Eco47I	GGWCC	2 cut(s)	1843, 2266
Eco47III	AGCGCT	1 cut(s)	1161
Eco53kI	GAGCTC	1 cut(s)	1306
Eco57I	CTGAAG	3 cut(s)	725, 2076, 2709
Eco88I	CYCGRG	2 cut(s)	411, 2628
EcoICRI	GAGCTC	1 cut(s)	1306
EcoNI	CCTNNNNNAGG	2 cut(s)	1151, 2552
EcoRI	GAATTC	2 cut(s)	682, 1416
EcoRII	CCWGG	5 cut(s)	123, 555, 1912, 2268, 3532
EcoT14I	CCWWGG	2 cut(s)	2359, 2721
EcoT22I	ATGCAT	3 cut(s)	2032, 3093, 3507
EcoT38I	GRGCYC	4 cut(s)	468, 694, 1039, 1308
ErhI	CCWWGG	2 cut(s)	2359, 2721
FaqI	GGGAC	3 cut(s)	2215, 2852, 2965
FauI	CCCGC	2 cut(s)	477, 2289
FauNDI	CATATG	4 cut(s)	1111, 2032, 2560, 2746
FbaI	TGATCA	2 cut(s)	2130, 2445
Fnu4HI	GCNGC	8 cut(s)	306, 473, 574, 1539, 1746, 2016, 2350, 2888
FokI	GGATG	9 cut(s)	302, 1061, 1217, 1256, 1595, 2120, 2291, 2692, 3007
FriOI	GRGCYC	4 cut(s)	468, 694, 1039, 1308
Fsp4HI	GCNGC	8 cut(s)	306, 473, 574, 1539, 1746, 2016, 2350, 2888
FspBI	CTAG	9 cut(s)	176, 627, 638, 717, 2324, 2618, 2831, 3048, 3835
GlaI	GCGC	6 cut(s)	953, 1161, 1892, 2353, 3102, 3604
GluI	GCNGC	8 cut(s)	306, 473, 574, 1539, 1746, 2016, 2350, 2888
GsaI	CCCAGC	1 cut(s)	940
HaeII	RGCGCY	2 cut(s)	1163, 3104
HaeIII	GGCC	5 cut(s)	543, 1146, 2209, 2364, 3812
HapII	CCGG	4 cut(s)	319, 459, 1202, 3748
HgaI	GACGC	3 cut(s)	1514, 1882, 3787
HhaI	GCGC	6 cut(s)	954, 1162, 1893, 2354, 3103, 3605
Hin1I	GRCGYC	2 cut(s)	1525, 3798
Hin6I	GCGC	6 cut(s)	952, 1160, 1891, 2352, 3101, 3603
HinP1I	GCGC	6 cut(s)	952, 1160, 1891, 2352, 3101, 3603
HindIII	AAGCTT	6 cut(s)	593, 737, 2136, 2153, 2166, 3140
HpaII	CCGG	4 cut(s)	319, 459, 1202, 3748
HphI	GGTGA	8 cut(s)	576, 896, 1346, 2003, 2104, 2267, 2695, 3055
Hpy166II	GTNNAC	6 cut(s)	64, 1290, 1389, 2266, 2389, 2572
Hpy8I	GTNNAC	6 cut(s)	64, 1290, 1389, 2266, 2389, 2572
Hpy99I	CGWCG	2 cut(s)	1898, 3544
HpyCH4IV	ACGT	5 cut(s)	1424, 2124, 3213, 3221, 3238
HpyF10VI	GCNNNNNNNGC	7 cut(s)	472, 638, 1312, 2333, 2342, 2351, 2355
HpySE526I	ACGT	5 cut(s)	1424, 2124, 3213, 3221, 3238
Hsp92I	GRCGYC	2 cut(s)	1525, 3798
HspAI	GCGC	6 cut(s)	952, 1160, 1891, 2352, 3101, 3603
Kpn2I	TCCGGA	1 cut(s)	1201
KroI	GCCGGC	1 cut(s)	3747
KroNI	GCCGGC	1 cut(s)	3749
Ksp22I	TGATCA	2 cut(s)	2130, 2445
LmnI	GCTCC	3 cut(s)	285, 513, 2585
Lsp1109I	GCAGC	6 cut(s)	292, 1525, 1732, 2027, 2336, 2899
MabI	ACCWGGT	1 cut(s)	3532
MaeI	CTAG	9 cut(s)	176, 627, 638, 717, 2324, 2618, 2831, 3048, 3835
MaeII	ACGT	5 cut(s)	1424, 2124, 3213, 3221, 3238
MaeIII	GTNAC	8 cut(s)	113, 521, 972, 1210, 1334, 2683, 3217, 3474
MflI	RGATCY	2 cut(s)	46, 2614
MhlI	GDGCHC	7 cut(s)	247, 468, 694, 1039, 1292, 1308, 2629
MlsI	TGGCCA	1 cut(s)	2364
MluNI	TGGCCA	1 cut(s)	2364
MlyI	GAGTC	7 cut(s)	490, 854, 1331, 1349, 2567, 3026, 3458
MmeI	TCCRAC	5 cut(s)	1046, 1617, 3617, 3706, 3720
Mox20I	TGGCCA	1 cut(s)	2364
Mph1103I	ATGCAT	3 cut(s)	2032, 3093, 3507
MroI	TCCGGA	1 cut(s)	1201
MroNI	GCCGGC	1 cut(s)	3747
MroXI	GAANNNNTTC	1 cut(s)	3176
MscI	TGGCCA	1 cut(s)	2364
MslI	CAYNNNNRTG	5 cut(s)	1008, 1925, 2240, 3179, 3185
Msp20I	TGGCCA	1 cut(s)	2364
MspA1I	CMGCKG	2 cut(s)	3292, 3424
MspI	CCGG	4 cut(s)	319, 459, 1202, 3748
MspR9I	CCNGG	5 cut(s)	125, 557, 1914, 2270, 3534
Mva1269I	GAATGC	2 cut(s)	1903, 3192
MvaI	CCWGG	5 cut(s)	125, 557, 1914, 2270, 3534
MvnI	CGCG	2 cut(s)	954, 1893
MwoI	GCNNNNNNNGC	7 cut(s)	472, 638, 1312, 2333, 2342, 2351, 2355
NaeI	GCCGGC	1 cut(s)	3749
NdeI	CATATG	4 cut(s)	1111, 2032, 2560, 2746
NgoMIV	GCCGGC	1 cut(s)	3747
NlaIV	GGNNCC	6 cut(s)	48, 427, 1575, 2626, 3207, 3642
NmuCI	GTSAC	3 cut(s)	972, 1334, 2683
NsiI	ATGCAT	3 cut(s)	2032, 3093, 3507
NspI	RCATGY	7 cut(s)	820, 2013, 2349, 2785, 3095, 3178, 3551
NspV	TTCGAA	1 cut(s)	598
PaeI	GCATGC	3 cut(s)	2349, 2785, 3095
PagI	TCATGA	2 cut(s)	2127, 3180
PceI	AGGCCT	1 cut(s)	1146
PciI	ACATGT	3 cut(s)	2009, 3174, 3547
PcsI	WCGNNNNNNNCGW	1 cut(s)	1471
PctI	GAATGC	2 cut(s)	1903, 3192
PdiI	GCCGGC	1 cut(s)	3749
PdmI	GAANNNNTTC	1 cut(s)	3176
PfeI	GAWTC	9 cut(s)	895, 916, 1325, 1864, 2510, 3107, 3146, 3671, 3729
PflMI	CCANNNNNTGG	2 cut(s)	2398, 2727
PkrI	GCNGC	8 cut(s)	307, 474, 575, 1540, 1747, 2017, 2351, 2889
PleI	GAGTC	7 cut(s)	489, 853, 1331, 1349, 2567, 3025, 3457
PpsI	GAGTC	7 cut(s)	489, 853, 1331, 1349, 2567, 3025, 3457
PscI	ACATGT	3 cut(s)	2009, 3174, 3547
PsiI	TTATAA	4 cut(s)	1008, 1278, 1589, 1701
Psp124BI	GAGCTC	1 cut(s)	1308
Psp6I	CCWGG	5 cut(s)	123, 555, 1912, 2268, 3532
PspFI	CCCAGC	1 cut(s)	936
PspGI	CCWGG	5 cut(s)	123, 555, 1912, 2268, 3532
PspN4I	GGNNCC	6 cut(s)	48, 427, 1575, 2626, 3207, 3642
PspPI	GGNCC	3 cut(s)	1843, 2208, 2266
PsrI	GAACNNNNNNTAC	4 cut(s)	45, 77, 3703, 3735
PsuI	RGATCY	2 cut(s)	46, 2614
PvuII	CAGCTG	1 cut(s)	3292
RsaI	GTAC	8 cut(s)	1511, 2390, 2595, 3237, 3329, 3484, 3531, 3552
RsaNI	GTAC	8 cut(s)	1510, 2389, 2594, 3236, 3328, 3483, 3530, 3551
RseI	CAYNNNNRTG	5 cut(s)	1008, 1925, 2240, 3179, 3185
SacI	GAGCTC	1 cut(s)	1308
SatI	GCNGC	8 cut(s)	306, 473, 574, 1539, 1746, 2016, 2350, 2888
Sau96I	GGNCC	3 cut(s)	1843, 2208, 2266
ScaI	AGTACT	1 cut(s)	2595
SchI	GAGTC	7 cut(s)	490, 854, 1331, 1349, 2567, 3026, 3458
ScrFI	CCNGG	5 cut(s)	125, 557, 1914, 2270, 3534
SduI	GDGCHC	7 cut(s)	247, 468, 694, 1039, 1292, 1308, 2629
SexAI	ACCWGGT	1 cut(s)	3532
SfcI	CTRYAG	3 cut(s)	39, 189, 2074
SfuI	TTCGAA	1 cut(s)	598
SinI	GGWCC	2 cut(s)	1843, 2266
SmiMI	CAYNNNNRTG	5 cut(s)	1008, 1925, 2240, 3179, 3185
SmlI	CTYRAG	1 cut(s)	920
SmoI	CTYRAG	1 cut(s)	920
SphI	GCATGC	3 cut(s)	2349, 2785, 3095
SseBI	AGGCCT	1 cut(s)	1146
SsiI	CCGC	9 cut(s)	396, 470, 473, 574, 1643, 2066, 2296, 2844, 3424
SspI	AATATT	1 cut(s)	3589
SspMI	CTAG	9 cut(s)	176, 627, 638, 717, 2324, 2618, 2831, 3048, 3835
SstI	GAGCTC	1 cut(s)	1308
StuI	AGGCCT	1 cut(s)	1146
StyD4I	CCNGG	5 cut(s)	123, 555, 1912, 2268, 3532
StyI	CCWWGG	2 cut(s)	2359, 2721
TaiI	ACGT	5 cut(s)	1427, 2127, 3216, 3224, 3241
TatI	WGTACW	4 cut(s)	2593, 3327, 3482, 3550
TauI	GCSGC	2 cut(s)	475, 576
TfiI	GAWTC	9 cut(s)	895, 916, 1325, 1864, 2510, 3107, 3146, 3671, 3729
TscAI	CASTG	4 cut(s)	749, 1297, 1396, 3485
TseFI	GTSAC	3 cut(s)	972, 1334, 2683
TseI	GCWGC	6 cut(s)	305, 1538, 1745, 2015, 2349, 2887
Tsp45I	GTSAC	3 cut(s)	972, 1334, 2683
TspGWI	ACGGA	3 cut(s)	2466, 3557, 3672
TspRI	CASTG	4 cut(s)	749, 1297, 1396, 3485
Van91I	CCANNNNNTGG	2 cut(s)	2398, 2727
VneI	GTGCAC	1 cut(s)	1288
VpaK11BI	GGWCC	2 cut(s)	1843, 2266
XagI	CCTNNNNNAGG	2 cut(s)	1151, 2552
XbaI	TCTAGA	1 cut(s)	2617
XceI	RCATGY	7 cut(s)	820, 2013, 2349, 2785, 3095, 3178, 3551
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3722
XmnI	GAANNNNTTC	1 cut(s)	3176
XspI	CTAG	9 cut(s)	176, 627, 638, 717, 2324, 2618, 2831, 3048, 3835
ZrmI	AGTACT	1 cut(s)	2595
Zsp2I	ATGCAT	3 cut(s)	2032, 3093, 3507

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.