MD09G1112200.v1.1

Protein of unknown function (DUF861)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008518
Physical Location & Seq
Forward (+)
8534954 .. 8536205
1252 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1112200.v1.1.491

Sequence Viewer

Length: 438 bp
ATGGCAAGTGTGATCATGGCCCCAACTTTCAGCTCACTCTCAGTCAGCAGAAATGGCAGAGATTGGAGCGAGCGGAGTGGTGCTAATGCACAACCTCGTGGTATGAAAGTGAAGGCAATGCGAGTAGAGAAACCTCTGGAGGAACTGTACCAAGTAAGAGTGGAAAGAAAGGTGTCACAGGAGAGACTAGCTGAGCTTGGAGTCTCGAGATGGTCAATGTGGAAGACGGGCAAGTGCAAGCTGCCGTGGGACTGGCAGGTGGACCAGCTGGTTTACATTGAGGAAGGAGAGGTGAGAGTCGTGCCTGAAGGCAGCCAGCAGTACATGCAATTTGTGGCCGGAGATCTTGTTCGTTATCCCAAGTGGCTGGAGGCTGACCTCTGGTTCAACGGTCCTTACCAAGAGCGTTACAGTTTCCGAGCCTACGGCGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

146

Amino Acids

16.96

Weight (kDa)

7.78

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 64 - 129 2.3e-18 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 247
Acc36I ACCTGC 1 cut(s) 247
AccBSI CCGCTC 1 cut(s) 73
AciI CCGC 1 cut(s) 73
AcoI YGGCCR 1 cut(s) 336
AcuI CTGAAG 1 cut(s) 327
AfaI GTAC 2 cut(s) 149, 323
AgsI TTSAA 1 cut(s) 388
AloI GAACNNNNNNTCC 2 cut(s) 333, 365
AluBI AGCT 5 cut(s) 33, 191, 196, 241, 268
AluI AGCT 5 cut(s) 33, 191, 196, 241, 268
Alw26I GTCTC 2 cut(s) 178, 208
Ama87I CYCGRG 1 cut(s) 205
AoxI GGCC 2 cut(s) 18, 336
ApeKI GCWGC 2 cut(s) 241, 312
AspS9I GGNCC 3 cut(s) 19, 262, 392
AsuHPI GGTGA 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 205
AvaII GGWCC 2 cut(s) 262, 392
BauI CACGAG 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 230
BbvI GCAGC 2 cut(s) 228, 324
BccI CCATC 1 cut(s) 204
BceAI ACGGC 1 cut(s) 229
BclI TGATCA 1 cut(s) 12
BcoDI GTCTC 2 cut(s) 178, 208
BfaI CTAG 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 247
BglII AGATCT 1 cut(s) 343
BisI GCNGC 2 cut(s) 242, 313
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 2 cut(s) 243, 314
Bme18I GGWCC 2 cut(s) 262, 392
BmeT110I CYCGRG 1 cut(s) 205
BmgT120I GGNCC 3 cut(s) 19, 262, 392
BmiI GGNNCC 1 cut(s) 21
BpiI GAAGAC 1 cut(s) 230
BpmI CTGGAG 2 cut(s) 158, 389
Bpu1102I GCTNAGC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 245
BsaXI ACNNNNNCTCC 4 cut(s) 131, 161, 333, 363
Bse1I ACTGG 1 cut(s) 257
Bse3DI GCAATG 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 245
BseMI GCAATG 1 cut(s) 123
BseMII CTCAG 2 cut(s) 54, 183
BseNI ACTGG 1 cut(s) 257
BseXI GCAGC 2 cut(s) 228, 324
BshFI GGCC 2 cut(s) 20, 338
BsiHKCI CYCGRG 1 cut(s) 205
BsiSI CCGG 1 cut(s) 339
BslFI GGGAC 1 cut(s) 263
BsmAI GTCTC 2 cut(s) 178, 208
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 2 cut(s) 20, 338
BsoBI CYCGRG 1 cut(s) 205
Bsp143I GATC 2 cut(s) 12, 343
Bsp1720I GCTNAGC 1 cut(s) 192
BspACI CCGC 1 cut(s) 73
BspANI GGCC 2 cut(s) 20, 338
BspCNI CTCAG 2 cut(s) 53, 184
BspLI GGNNCC 1 cut(s) 21
BspMI ACCTGC 1 cut(s) 247
BsrBI CCGCTC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 123
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 1 cut(s) 245
BssMI GATC 2 cut(s) 12, 343
BssSI CACGAG 1 cut(s) 96
Bst2BI CACGAG 1 cut(s) 96
Bst4CI ACNGT 3 cut(s) 147, 392, 413
BstAPI GCANNNNNTGC 1 cut(s) 325
BstC8I GCNNGC 3 cut(s) 71, 239, 317
BstDEI CTNAG 2 cut(s) 40, 192
BstDSI CCRYGG 1 cut(s) 245
BstKTI GATC 2 cut(s) 15, 346
BstMAI GTCTC 2 cut(s) 178, 208
BstMBI GATC 2 cut(s) 12, 343
BstMWI GCNNNNNNNGC 2 cut(s) 54, 325
BstNSI RCATGY 1 cut(s) 328
BstV1I GCAGC 2 cut(s) 228, 324
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 1 cut(s) 343
BstXI CCANNNNNNTGG 1 cut(s) 367
BstYI RGATCY 1 cut(s) 343
BsuRI GGCC 2 cut(s) 20, 338
BtgI CCRYGG 1 cut(s) 245
BveI ACCTGC 1 cut(s) 247
Cac8I GCNNGC 3 cut(s) 71, 239, 317
Cfr13I GGNCC 3 cut(s) 19, 262, 392
Csp6I GTAC 2 cut(s) 148, 322
CviAII CATG 2 cut(s) 16, 325
CviQI GTAC 2 cut(s) 148, 322
DdeI CTNAG 2 cut(s) 40, 192
DpnI GATC 2 cut(s) 14, 345
DpnII GATC 2 cut(s) 12, 343
EaeI YGGCCR 1 cut(s) 336
Eco47I GGWCC 2 cut(s) 262, 392
Eco57I CTGAAG 1 cut(s) 327
Eco88I CYCGRG 1 cut(s) 205
FaeI CATG 2 cut(s) 19, 328
FaiI YATR 3 cut(s) 17, 104, 326
FaqI GGGAC 1 cut(s) 263
FatI CATG 2 cut(s) 15, 324
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 2 cut(s) 242, 313
Fsp4HI GCNGC 2 cut(s) 242, 313
FspBI CTAG 1 cut(s) 188
GluI GCNGC 2 cut(s) 242, 313
GsuI CTGGAG 2 cut(s) 158, 389
HaeIII GGCC 2 cut(s) 20, 338
HapII CCGG 1 cut(s) 339
Hin1II CATG 2 cut(s) 19, 328
HinfI GANTC 2 cut(s) 201, 297
HpaII CCGG 1 cut(s) 339
HphI GGTGA 1 cut(s) 304
Hpy166II GTNNAC 2 cut(s) 262, 274
Hpy188I TCNGA 1 cut(s) 419
Hpy188III TCNNGA 3 cut(s) 137, 205, 207
Hpy8I GTNNAC 2 cut(s) 262, 274
HpyAV CCTTC 3 cut(s) 106, 278, 302
HpyCH4III ACNGT 3 cut(s) 147, 392, 413
HpyCH4V TGCA 3 cut(s) 89, 237, 328
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 325
HpyF3I CTNAG 2 cut(s) 40, 192
Hsp92II CATG 2 cut(s) 19, 328
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 2 cut(s) 12, 343
LmnI GCTCC 1 cut(s) 66
Lsp1109I GCAGC 2 cut(s) 228, 324
MaeI CTAG 1 cut(s) 188
MaeIII GTNAC 2 cut(s) 174, 407
MalI GATC 2 cut(s) 14, 345
MbiI CCGCTC 1 cut(s) 73
MboI GATC 2 cut(s) 12, 343
MboII GAAGA 1 cut(s) 235
MflI RGATCY 1 cut(s) 343
MluCI AATT 1 cut(s) 329
MlyI GAGTC 2 cut(s) 210, 306
MnlI CCTC 7 cut(s) 105, 133, 144, 274, 283, 364, 389
MspA1I CMGCKG 1 cut(s) 268
MspI CCGG 1 cut(s) 339
MwoI GCNNNNNNNGC 2 cut(s) 54, 325
NdeII GATC 2 cut(s) 12, 343
NlaIII CATG 2 cut(s) 19, 328
NlaIV GGNNCC 1 cut(s) 21
NmuCI GTSAC 1 cut(s) 174
NspI RCATGY 1 cut(s) 328
PaeR7I CTCGAG 1 cut(s) 205
PaqCI CACCTGC 1 cut(s) 247
PkrI GCNGC 2 cut(s) 243, 314
PleI GAGTC 2 cut(s) 209, 305
PpsI GAGTC 2 cut(s) 209, 305
PspN4I GGNNCC 1 cut(s) 21
PspPI GGNCC 3 cut(s) 19, 262, 392
PsuI RGATCY 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 268
RsaI GTAC 2 cut(s) 149, 323
RsaNI GTAC 2 cut(s) 148, 322
SatI GCNGC 2 cut(s) 242, 313
Sau3AI GATC 2 cut(s) 12, 343
Sau96I GGNCC 3 cut(s) 19, 262, 392
SchI GAGTC 2 cut(s) 210, 306
Sfr274I CTCGAG 1 cut(s) 205
SinI GGWCC 2 cut(s) 262, 392
SlaI CTCGAG 1 cut(s) 205
SmlI CTYRAG 1 cut(s) 205
SmoI CTYRAG 1 cut(s) 205
Sse9I AATT 1 cut(s) 329
SsiI CCGC 1 cut(s) 73
SspMI CTAG 1 cut(s) 188
TaaI ACNGT 3 cut(s) 147, 392, 413
TaqI TCGA 1 cut(s) 206
TasI AATT 1 cut(s) 329
TatI WGTACW 1 cut(s) 321
TseFI GTSAC 1 cut(s) 174
TseI GCWGC 2 cut(s) 241, 312
Tsp45I GTSAC 1 cut(s) 174
TspDTI ATGAA 1 cut(s) 119
VpaK11BI GGWCC 2 cut(s) 262, 392
XceI RCATGY 1 cut(s) 328
XhoI CTCGAG 1 cut(s) 205
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.