MD17G1102300.v1.1

Protein of unknown function (DUF861)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008518
Physical Location & Seq
Forward (+)
8703120 .. 8705388
2269 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1102300.v1.1.491

Sequence Viewer

Length: 423 bp
ATGGCCCCAACTTTCTGCTCAATCTCAGTCAGAAGAAGCGGCAAAGATTGGAGCGAGCGCAGTAGTACTAACGGGCAACATCGTGGTATGAAATTGAAGGCAATGCGGATAGAGAAACCTCTGGAGGAATTGTACCAAGTGAGAGTCGAGAGAAAGGTGTCACAGGAGAGACTAGCAGAGCTTGGAGTCTCCAGATGGTCAATGTGGAAGACGGGCAAGTGCAAGCTGCCGTGGGACTGGCAGGTCGACCAGCTGGTTTACATTGAGGAAGGAGAGGTGAGAGTCGTGTCCGAAGGCAGCCGGCAGTACATGCAATTCGTGGCTGGGGATCTTATTCGTTACCCCAAGTGGTTGGAGGCTGACCTCTGGTTCAACGGTCCTTACCAAGAGCGTTACAGTTTCCGAGCCTTTGGCGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

141

Amino Acids

16.62

Weight (kDa)

8.9

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 59 - 124 1.4e-16 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 232
AccI GTMKAC 1 cut(s) 246
AciI CCGC 2 cut(s) 39, 106
AclWI GGATC 1 cut(s) 336
AfaI GTAC 3 cut(s) 67, 134, 308
AgsI TTSAA 2 cut(s) 97, 373
AluBI AGCT 3 cut(s) 181, 226, 253
AluI AGCT 3 cut(s) 181, 226, 253
Alw26I GTCTC 2 cut(s) 163, 193
AlwI GGATC 1 cut(s) 336
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 226, 297
AspLEI GCGC 1 cut(s) 60
AspS9I GGNCC 2 cut(s) 4, 377
AsuHPI GGTGA 1 cut(s) 289
AvaII GGWCC 1 cut(s) 377
BbsI GAAGAC 1 cut(s) 215
BbvI GCAGC 2 cut(s) 213, 309
BccI CCATC 1 cut(s) 189
BceAI ACGGC 1 cut(s) 214
BcoDI GTCTC 2 cut(s) 163, 193
BfaI CTAG 1 cut(s) 173
BfuAI ACCTGC 1 cut(s) 232
BisI GCNGC 3 cut(s) 40, 227, 298
BlsI GCNGC 3 cut(s) 41, 228, 299
BmcAI AGTACT 1 cut(s) 67
Bme18I GGWCC 1 cut(s) 377
BmgT120I GGNCC 2 cut(s) 4, 377
BmiI GGNNCC 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 215
BpmI CTGGAG 2 cut(s) 143, 175
BsaJI CCNNGG 1 cut(s) 230
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bse118I RCCGGY 1 cut(s) 300
Bse1I ACTGG 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 230
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 1 cut(s) 39
BseNI ACTGG 1 cut(s) 242
BseXI GCAGC 2 cut(s) 213, 309
BseYI CCCAGC 1 cut(s) 323
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 301
BslFI GGGAC 1 cut(s) 248
BsmAI GTCTC 2 cut(s) 163, 193
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 328
BspACI CCGC 2 cut(s) 39, 106
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 38
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 232
BspPI GGATC 1 cut(s) 336
BsrDI GCAATG 1 cut(s) 108
BsrFI RCCGGY 1 cut(s) 300
BsrI ACTGG 1 cut(s) 242
BssAI RCCGGY 1 cut(s) 300
BssECI CCNNGG 1 cut(s) 230
BssMI GATC 1 cut(s) 328
Bst4CI ACNGT 2 cut(s) 377, 398
BstAPI GCANNNNNTGC 1 cut(s) 310
BstC8I GCNNGC 3 cut(s) 56, 224, 302
BstDEI CTNAG 1 cut(s) 25
BstDSI CCRYGG 1 cut(s) 230
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 1 cut(s) 331
BstMAI GTCTC 2 cut(s) 163, 193
BstMBI GATC 1 cut(s) 328
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstNSI RCATGY 1 cut(s) 313
BstV1I GCAGC 2 cut(s) 213, 309
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 1 cut(s) 328
BstXI CCANNNNNNTGG 1 cut(s) 352
BstYI RGATCY 1 cut(s) 328
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 230
BveI ACCTGC 1 cut(s) 232
Cac8I GCNNGC 3 cut(s) 56, 224, 302
CfoI GCGC 1 cut(s) 60
Cfr10I RCCGGY 1 cut(s) 300
Cfr13I GGNCC 2 cut(s) 4, 377
Csp6I GTAC 3 cut(s) 66, 133, 307
CviAII CATG 1 cut(s) 310
CviJI RGCY 8 cut(s) 5, 181, 226, 253, 300, 323, 359, 407
CviKI_1 RGCY 8 cut(s) 5, 181, 226, 253, 300, 323, 359, 407
CviQI GTAC 3 cut(s) 66, 133, 307
DdeI CTNAG 1 cut(s) 25
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 377
FaeI CATG 1 cut(s) 313
FaiI YATR 2 cut(s) 89, 311
FaqI GGGAC 1 cut(s) 248
FatI CATG 1 cut(s) 309
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 3 cut(s) 40, 227, 298
Fsp4HI GCNGC 3 cut(s) 40, 227, 298
FspBI CTAG 1 cut(s) 173
GlaI GCGC 1 cut(s) 59
GluI GCNGC 3 cut(s) 40, 227, 298
GsaI CCCAGC 1 cut(s) 327
GsuI CTGGAG 2 cut(s) 143, 175
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 301
HhaI GCGC 1 cut(s) 60
Hin1II CATG 1 cut(s) 313
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 3 cut(s) 144, 186, 282
HpaII CCGG 1 cut(s) 301
HphI GGTGA 1 cut(s) 289
Hpy166II GTNNAC 2 cut(s) 247, 259
Hpy188I TCNGA 3 cut(s) 32, 292, 404
Hpy188III TCNNGA 3 cut(s) 122, 148, 192
Hpy8I GTNNAC 2 cut(s) 247, 259
HpyAV CCTTC 3 cut(s) 91, 263, 287
HpyCH4III ACNGT 2 cut(s) 377, 398
HpyCH4V TGCA 2 cut(s) 222, 313
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpyF3I CTNAG 1 cut(s) 25
Hsp92II CATG 1 cut(s) 313
HspAI GCGC 1 cut(s) 58
KroI GCCGGC 1 cut(s) 300
KroNI GCCGGC 1 cut(s) 302
Kzo9I GATC 1 cut(s) 328
LmnI GCTCC 1 cut(s) 51
Lsp1109I GCAGC 2 cut(s) 213, 309
MaeI CTAG 1 cut(s) 173
MaeIII GTNAC 3 cut(s) 159, 338, 392
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 2 cut(s) 45, 220
MflI RGATCY 1 cut(s) 328
MluCI AATT 3 cut(s) 92, 128, 314
MlyI GAGTC 3 cut(s) 153, 195, 291
MmeI TCCRAC 1 cut(s) 333
MnlI CCTC 6 cut(s) 118, 129, 259, 268, 349, 374
MroNI GCCGGC 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 253
MspI CCGG 1 cut(s) 301
MwoI GCNNNNNNNGC 1 cut(s) 310
NaeI GCCGGC 1 cut(s) 302
NdeII GATC 1 cut(s) 328
NgoMIV GCCGGC 1 cut(s) 300
NlaIII CATG 1 cut(s) 313
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 159
NspI RCATGY 1 cut(s) 313
PdiI GCCGGC 1 cut(s) 302
PkrI GCNGC 3 cut(s) 41, 228, 299
PleI GAGTC 3 cut(s) 152, 194, 290
PpsI GAGTC 3 cut(s) 152, 194, 290
PspFI CCCAGC 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 4, 377
PsuI RGATCY 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 253
RsaI GTAC 3 cut(s) 67, 134, 308
RsaNI GTAC 3 cut(s) 66, 133, 307
SalI GTCGAC 1 cut(s) 245
SatI GCNGC 3 cut(s) 40, 227, 298
Sau3AI GATC 1 cut(s) 328
Sau96I GGNCC 2 cut(s) 4, 377
ScaI AGTACT 1 cut(s) 67
SchI GAGTC 3 cut(s) 153, 195, 291
SetI ASST 8 cut(s) 121, 159, 183, 228, 246, 255, 279, 366
SinI GGWCC 1 cut(s) 377
Sse9I AATT 3 cut(s) 92, 128, 314
SsiI CCGC 2 cut(s) 39, 106
SspMI CTAG 1 cut(s) 173
TaaI ACNGT 2 cut(s) 377, 398
TaqI TCGA 2 cut(s) 147, 246
TasI AATT 3 cut(s) 92, 128, 314
TatI WGTACW 2 cut(s) 65, 306
TauI GCSGC 1 cut(s) 42
TseFI GTSAC 1 cut(s) 159
TseI GCWGC 2 cut(s) 226, 297
Tsp45I GTSAC 1 cut(s) 159
TspDTI ATGAA 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 377
XceI RCATGY 1 cut(s) 313
XmiI GTMKAC 1 cut(s) 246
XspI CTAG 1 cut(s) 173
ZrmI AGTACT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.