pycom09g03670

Protein of unknown function (DUF861)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0008518
Physical Location & Seq
Forward (+)
2716001 .. 2716438
438 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g03670.1

Sequence Viewer

Length: 438 bp
ATGGCAAGTGTGATCATGGCCCCAACTTTCAGCTCACTCTCATTCAGCAGAAGTGGCAGAGATTGGAGCGAGCGGAGTAGTGCTAATGCACAACCTCGCGGTATGACAGTGAAGGCAATGCGAATAGAGAAACCTCTGGAGGAACTGTACCAAGTAAGAGTGGAAAGAAGGGTGTCACAGGAGAGACTAGCAGAGCTTGGAGTCTCGAGATGGTCAATGTGGAAGACGGGTAAGTGCAAGCTGCCGTGGGACTGGCAGGTGGACCAGCTGGTTTACATTGAGGAAGGAGAGGTGAGAGTCGTGCCTGAAGGCAGCCAGCAGTACATGCAATTTGTGGCAGGAGATCTTGTTCGTTATCCCAAGTGGCTGGAGGCTGACCTCTGGTTCAATGGTCCTTACCAAGAGCGTTACAGTTTCCGAGCCTATGGCGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

146

Amino Acids

17.03

Weight (kDa)

6.38

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 64 - 129 2.3e-18 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 247
Acc36I ACCTGC 1 cut(s) 247
AccBSI CCGCTC 1 cut(s) 73
AccII CGCG 1 cut(s) 99
AciI CCGC 2 cut(s) 73, 99
AcuI CTGAAG 1 cut(s) 327
AfaI GTAC 2 cut(s) 149, 323
AgsI TTSAA 1 cut(s) 388
AloI GAACNNNNNNTCC 2 cut(s) 333, 365
AluBI AGCT 4 cut(s) 33, 196, 241, 268
AluI AGCT 4 cut(s) 33, 196, 241, 268
Alw26I GTCTC 2 cut(s) 178, 208
Ama87I CYCGRG 1 cut(s) 205
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 2 cut(s) 241, 312
AspS9I GGNCC 3 cut(s) 19, 262, 392
AsuHPI GGTGA 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 205
AvaII GGWCC 2 cut(s) 262, 392
BbsI GAAGAC 1 cut(s) 230
BbvI GCAGC 2 cut(s) 228, 324
BccI CCATC 1 cut(s) 204
BceAI ACGGC 1 cut(s) 229
BclI TGATCA 1 cut(s) 12
BcoDI GTCTC 2 cut(s) 178, 208
BfaI CTAG 1 cut(s) 188
BfuAI ACCTGC 1 cut(s) 247
BglII AGATCT 1 cut(s) 343
BisI GCNGC 2 cut(s) 242, 313
BlsI GCNGC 2 cut(s) 243, 314
Bme18I GGWCC 2 cut(s) 262, 392
BmeT110I CYCGRG 1 cut(s) 205
BmgT120I GGNCC 3 cut(s) 19, 262, 392
BmiI GGNNCC 1 cut(s) 21
BpiI GAAGAC 1 cut(s) 230
BpmI CTGGAG 2 cut(s) 158, 389
BsaJI CCNNGG 1 cut(s) 245
BsaXI ACNNNNNCTCC 4 cut(s) 131, 161, 333, 363
Bse1I ACTGG 1 cut(s) 257
Bse3DI GCAATG 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 245
BseMI GCAATG 1 cut(s) 123
BseNI ACTGG 1 cut(s) 257
BseXI GCAGC 2 cut(s) 228, 324
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 20
BsiHKCI CYCGRG 1 cut(s) 205
BslFI GGGAC 1 cut(s) 263
BsmAI GTCTC 2 cut(s) 178, 208
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 205
Bsp143I GATC 2 cut(s) 12, 343
BspACI CCGC 2 cut(s) 73, 99
BspANI GGCC 1 cut(s) 20
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 21
BspMI ACCTGC 1 cut(s) 247
BsrBI CCGCTC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 123
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 1 cut(s) 245
BssMI GATC 2 cut(s) 12, 343
Bst4CI ACNGT 3 cut(s) 109, 147, 413
BstAPI GCANNNNNTGC 1 cut(s) 325
BstC8I GCNNGC 3 cut(s) 71, 239, 317
BstDSI CCRYGG 1 cut(s) 245
BstFNI CGCG 1 cut(s) 99
BstKTI GATC 2 cut(s) 15, 346
BstMAI GTCTC 2 cut(s) 178, 208
BstMBI GATC 2 cut(s) 12, 343
BstMWI GCNNNNNNNGC 2 cut(s) 54, 325
BstNSI RCATGY 1 cut(s) 328
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 228, 324
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 1 cut(s) 343
BstXI CCANNNNNNTGG 1 cut(s) 367
BstYI RGATCY 1 cut(s) 343
BsuRI GGCC 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 245
BtsIMutI CAGTG 1 cut(s) 114
BveI ACCTGC 1 cut(s) 247
Cac8I GCNNGC 3 cut(s) 71, 239, 317
Cfr13I GGNCC 3 cut(s) 19, 262, 392
Csp6I GTAC 2 cut(s) 148, 322
CviAII CATG 2 cut(s) 16, 325
CviJI RGCY 9 cut(s) 20, 33, 196, 241, 268, 315, 367, 374, 422
CviKI_1 RGCY 9 cut(s) 20, 33, 196, 241, 268, 315, 367, 374, 422
CviQI GTAC 2 cut(s) 148, 322
DpnI GATC 2 cut(s) 14, 345
DpnII GATC 2 cut(s) 12, 343
Eco47I GGWCC 2 cut(s) 262, 392
Eco57I CTGAAG 1 cut(s) 327
Eco88I CYCGRG 1 cut(s) 205
FaeI CATG 2 cut(s) 19, 328
FaiI YATR 4 cut(s) 17, 104, 326, 426
FaqI GGGAC 1 cut(s) 263
FatI CATG 2 cut(s) 15, 324
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 2 cut(s) 242, 313
Fsp4HI GCNGC 2 cut(s) 242, 313
FspBI CTAG 1 cut(s) 188
GluI GCNGC 2 cut(s) 242, 313
GsuI CTGGAG 2 cut(s) 158, 389
HaeIII GGCC 1 cut(s) 20
Hin1II CATG 2 cut(s) 19, 328
HinfI GANTC 2 cut(s) 201, 297
HphI GGTGA 1 cut(s) 304
Hpy166II GTNNAC 2 cut(s) 262, 274
Hpy188I TCNGA 1 cut(s) 419
Hpy188III TCNNGA 3 cut(s) 137, 205, 207
Hpy8I GTNNAC 2 cut(s) 262, 274
HpyAV CCTTC 4 cut(s) 106, 162, 278, 302
HpyCH4III ACNGT 3 cut(s) 109, 147, 413
HpyCH4V TGCA 3 cut(s) 89, 237, 328
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 325
Hsp92II CATG 2 cut(s) 19, 328
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 2 cut(s) 12, 343
LmnI GCTCC 1 cut(s) 66
Lsp1109I GCAGC 2 cut(s) 228, 324
MaeI CTAG 1 cut(s) 188
MaeIII GTNAC 2 cut(s) 174, 407
MalI GATC 2 cut(s) 14, 345
MbiI CCGCTC 1 cut(s) 73
MboI GATC 2 cut(s) 12, 343
MboII GAAGA 1 cut(s) 235
MflI RGATCY 1 cut(s) 343
MluCI AATT 1 cut(s) 329
MlyI GAGTC 2 cut(s) 210, 306
MnlI CCTC 7 cut(s) 105, 133, 144, 274, 283, 364, 389
MspA1I CMGCKG 1 cut(s) 268
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 2 cut(s) 54, 325
NdeII GATC 2 cut(s) 12, 343
NlaIII CATG 2 cut(s) 19, 328
NlaIV GGNNCC 1 cut(s) 21
NmuCI GTSAC 1 cut(s) 174
NspI RCATGY 1 cut(s) 328
PaeR7I CTCGAG 1 cut(s) 205
PaqCI CACCTGC 1 cut(s) 247
PkrI GCNGC 2 cut(s) 243, 314
PleI GAGTC 2 cut(s) 209, 305
PpsI GAGTC 2 cut(s) 209, 305
PspN4I GGNNCC 1 cut(s) 21
PspPI GGNCC 3 cut(s) 19, 262, 392
PsuI RGATCY 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 268
RsaI GTAC 2 cut(s) 149, 323
RsaNI GTAC 2 cut(s) 148, 322
SatI GCNGC 2 cut(s) 242, 313
Sau3AI GATC 2 cut(s) 12, 343
Sau96I GGNCC 3 cut(s) 19, 262, 392
SchI GAGTC 2 cut(s) 210, 306
SetI ASST 9 cut(s) 35, 97, 136, 198, 243, 261, 270, 294, 381
Sfr274I CTCGAG 1 cut(s) 205
SinI GGWCC 2 cut(s) 262, 392
SlaI CTCGAG 1 cut(s) 205
SmlI CTYRAG 1 cut(s) 205
SmoI CTYRAG 1 cut(s) 205
Sse9I AATT 1 cut(s) 329
SsiI CCGC 2 cut(s) 73, 99
SspMI CTAG 1 cut(s) 188
TaaI ACNGT 3 cut(s) 109, 147, 413
TaqI TCGA 1 cut(s) 206
TasI AATT 1 cut(s) 329
TatI WGTACW 1 cut(s) 321
TscAI CASTG 1 cut(s) 114
TseFI GTSAC 1 cut(s) 174
TseI GCWGC 2 cut(s) 241, 312
Tsp45I GTSAC 1 cut(s) 174
TspRI CASTG 1 cut(s) 114
VpaK11BI GGWCC 2 cut(s) 262, 392
XceI RCATGY 1 cut(s) 328
XhoI CTCGAG 1 cut(s) 205
XspI CTAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.