RchiOBHm_Chr2g0158451

Protein of unknown function (DUF861)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008518
Physical Location & Seq
Reverse (-)
74732772 .. 74733960
1189 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52713

Sequence Viewer

Length: 438 bp
ATGGCGAGTGTGATTATGGCCCCAACCTTGGGCTCATTCTCTGTAAACAGAAGCAGCAGAGATTGCAGCAAATGGAGTAGTACTAATGGGAAACGCAGTGGTCTAACGGTGAAGGCAATGCGGGTGGAGAAAAGTCTGGAGGAATTGTATGAAGTTAGAGTCGAGCGCAAAGTGTCGCCTGAACGAGTAGCAGAGCTTGGAGTTTCAAGGTGGTCAACATGGAAGACCGGCAAGTGCAAGTTGCCGTGGGACTGGCAGGTGGACCAGCTGGTTTACATTGAGGAAGGAGAGGTAAGGGTTGTGCCGGAAGGAAGCAAGAAGTTCATGCGATTTGTGGCTGGAGATCTTGTTCGTTACCCCAAGTGGTTTGAAGCTGACCTATGGTTCAATGGTCCATACCAAGAGCGTTACAGTTTCAGAGCATATGGAGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

145

Amino Acids

16.86

Weight (kDa)

9.1

Isoelectric Point (pI)

36.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 64 - 129 2.7e-18 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 247
Acc36I ACCTGC 1 cut(s) 247
AciI CCGC 1 cut(s) 121
AfaI GTAC 1 cut(s) 82
AfiI CCNNNNNNNGG 2 cut(s) 28, 29
AgsI TTSAA 3 cut(s) 207, 371, 388
AloI GAACNNNNNNTCC 2 cut(s) 333, 365
AluBI AGCT 3 cut(s) 196, 268, 374
AluI AGCT 3 cut(s) 196, 268, 374
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 2 cut(s) 54, 66
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 3 cut(s) 19, 262, 392
AsuHPI GGTGA 1 cut(s) 121
AvaII GGWCC 2 cut(s) 262, 392
BanII GRGCYC 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 230
BbvI GCAGC 2 cut(s) 66, 78
BceAI ACGGC 1 cut(s) 229
BfuAI ACCTGC 1 cut(s) 247
BglII AGATCT 1 cut(s) 343
BisI GCNGC 2 cut(s) 55, 67
BlsI GCNGC 2 cut(s) 56, 68
BmcAI AGTACT 1 cut(s) 82
Bme18I GGWCC 2 cut(s) 262, 392
BmgT120I GGNCC 3 cut(s) 19, 262, 392
BmiI GGNNCC 1 cut(s) 21
BpiI GAAGAC 1 cut(s) 230
BpmI CTGGAG 2 cut(s) 158, 360
BsaJI CCNNGG 2 cut(s) 27, 245
BsaXI ACNNNNNCTCC 4 cut(s) 131, 161, 333, 363
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 29
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 257
Bse3DI GCAATG 1 cut(s) 123
BseDI CCNNGG 2 cut(s) 27, 245
BseLI CCNNNNNNNGG 2 cut(s) 28, 29
BseMI GCAATG 1 cut(s) 123
BseNI ACTGG 1 cut(s) 257
BseXI GCAGC 2 cut(s) 66, 78
BshFI GGCC 1 cut(s) 20
BsiSI CCGG 2 cut(s) 228, 305
BslFI GGGAC 1 cut(s) 263
BslI CCNNNNNNNGG 2 cut(s) 28, 29
BsmFI GGGAC 1 cut(s) 263
BsnI GGCC 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 35
Bsp143I GATC 1 cut(s) 343
BspACI CCGC 1 cut(s) 121
BspANI GGCC 1 cut(s) 20
BspLI GGNNCC 1 cut(s) 21
BspMI ACCTGC 1 cut(s) 247
BsrDI GCAATG 1 cut(s) 123
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 1 cut(s) 257
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 2 cut(s) 27, 245
BssMI GATC 1 cut(s) 343
BssT1I CCWWGG 1 cut(s) 27
Bst4CI ACNGT 2 cut(s) 109, 413
BstAPI GCANNNNNTGC 1 cut(s) 63
BstDSI CCRYGG 1 cut(s) 245
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 1 cut(s) 346
BstMBI GATC 1 cut(s) 343
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstV1I GCAGC 2 cut(s) 66, 78
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
BsuRI GGCC 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 245
BtsI GCAGTG 1 cut(s) 103
BtsIMutI CAGTG 1 cut(s) 103
BveI ACCTGC 1 cut(s) 247
CfoI GCGC 1 cut(s) 168
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 3 cut(s) 19, 262, 392
Csp6I GTAC 1 cut(s) 81
CspCI CAANNNNNGTGG 2 cut(s) 105, 140
CviAII CATG 2 cut(s) 219, 325
CviJI RGCY 6 cut(s) 20, 33, 196, 268, 338, 374
CviKI_1 RGCY 6 cut(s) 20, 33, 196, 268, 338, 374
CviQI GTAC 1 cut(s) 81
DpnI GATC 1 cut(s) 345
DpnII GATC 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 27
Eco24I GRGCYC 1 cut(s) 35
Eco47I GGWCC 2 cut(s) 262, 392
EcoT14I CCWWGG 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 35
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 2 cut(s) 222, 328
FaiI YATR 8 cut(s) 17, 150, 220, 326, 382, 397, 424, 426
FaqI GGGAC 1 cut(s) 263
FatI CATG 2 cut(s) 218, 324
FauI CCCGC 1 cut(s) 114
FauNDI CATATG 1 cut(s) 424
Fnu4HI GCNGC 2 cut(s) 55, 67
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 2 cut(s) 55, 67
GlaI GCGC 1 cut(s) 167
GluI GCNGC 2 cut(s) 55, 67
GsuI CTGGAG 2 cut(s) 158, 360
HaeIII GGCC 1 cut(s) 20
HapII CCGG 2 cut(s) 228, 305
HhaI GCGC 1 cut(s) 168
Hin1II CATG 2 cut(s) 222, 328
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HinfI GANTC 1 cut(s) 159
HpaII CCGG 2 cut(s) 228, 305
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 4 cut(s) 46, 216, 262, 274
Hpy188I TCNGA 1 cut(s) 419
Hpy188III TCNNGA 1 cut(s) 137
Hpy8I GTNNAC 4 cut(s) 46, 216, 262, 274
HpyAV CCTTC 3 cut(s) 106, 278, 302
HpyCH4III ACNGT 2 cut(s) 109, 413
HpyCH4V TGCA 2 cut(s) 66, 237
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
Hsp92II CATG 2 cut(s) 222, 328
HspAI GCGC 1 cut(s) 166
Kzo9I GATC 1 cut(s) 343
LpnPI CCDG 9 cut(s) 122, 192, 238, 241, 242, 254, 278, 318, 324
Lsp1109I GCAGC 2 cut(s) 66, 78
MaeIII GTNAC 2 cut(s) 353, 407
MalI GATC 1 cut(s) 345
MboI GATC 1 cut(s) 343
MboII GAAGA 1 cut(s) 235
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 1 cut(s) 143
MlyI GAGTC 1 cut(s) 168
MnlI CCTC 3 cut(s) 133, 274, 283
MseI TTAA 1 cut(s) 436
MspA1I CMGCKG 1 cut(s) 268
MspI CCGG 2 cut(s) 228, 305
MwoI GCNNNNNNNGC 1 cut(s) 63
NdeI CATATG 1 cut(s) 424
NdeII GATC 1 cut(s) 343
NlaIII CATG 2 cut(s) 222, 328
NlaIV GGNNCC 1 cut(s) 21
PaqCI CACCTGC 1 cut(s) 247
PkrI GCNGC 2 cut(s) 56, 68
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
PspN4I GGNNCC 1 cut(s) 21
PspPI GGNCC 3 cut(s) 19, 262, 392
PsuI RGATCY 1 cut(s) 343
PvuII CAGCTG 1 cut(s) 268
RsaI GTAC 1 cut(s) 82
RsaNI GTAC 1 cut(s) 81
SaqAI TTAA 1 cut(s) 436
SatI GCNGC 2 cut(s) 55, 67
Sau3AI GATC 1 cut(s) 343
Sau96I GGNCC 3 cut(s) 19, 262, 392
ScaI AGTACT 1 cut(s) 82
SchI GAGTC 1 cut(s) 168
SduI GDGCHC 1 cut(s) 35
SetI ASST 8 cut(s) 29, 198, 212, 261, 270, 294, 376, 381
SinI GGWCC 2 cut(s) 262, 392
Sse9I AATT 1 cut(s) 143
SsiI CCGC 1 cut(s) 121
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 2 cut(s) 109, 413
TaqI TCGA 1 cut(s) 162
TasI AATT 1 cut(s) 143
TatI WGTACW 1 cut(s) 80
Tru1I TTAA 1 cut(s) 436
Tru9I TTAA 1 cut(s) 436
TscAI CASTG 1 cut(s) 103
TseI GCWGC 2 cut(s) 54, 66
TspDTI ATGAA 2 cut(s) 165, 313
TspRI CASTG 1 cut(s) 103
VpaK11BI GGWCC 2 cut(s) 262, 392
ZrmI AGTACT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.