Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0009264

Physical Location & Seq

Genomic Coordinates Reverse (-)

9421880 .. 9427733

Gene Structure 5854 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1122200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2430 bp

ATGCACAGGCTCCCACGACGCTCCGTCTCCGCCATCCTGCGCCACGGCGGGGCACGTTACCGGAACTCTGCGGCTCTGATCTCTTGCGCTTCTACCCACTGCGGCTCGGTGGGAGAGACTGATGGGAAAGTCAGATGGCACTCCGTGTTGGTCCCTGGGAAGTGTAACCCTACCAATTCCACGACTCAGTTTAATTTAAAAAATGGCATGTATTTTGGCAATCGGTATGAGTCCACGGCTGCGGCCTCTGATGCACCACCCGCGGAGACATACGAGTATCAAGCTGAGGTCAGTCGTCTCATGGACCTCATCGTTAACAGCTTGTACAGTAACAAGGAAGTTTTTCTTCGAGAGCTTATCAGCAATGCAAGTGATGCATTGGACAAGCTCCGTTTCCTCAGTGTCACACAGCCTGAGCTTTTGAAGGGTGGAGGTGATCTAGATATCCGTATCCAGACTGATAAAGACAATGGAATCATCAATATCATTGATTCTGGAATCGGTATGACTCGGCAAGAGCTTATTGACTGTCTGGGAACAATTGCACAAAGTGGGACTTCAAAGTTCTTGAAGGCATTGAAGGATAGTAAAGATGCTGGCAGTGATACCAATCTGATTGGTCAGTTTGGGGTCGGCTTTTACTCAGCATTCCTTGTTGCAGATCGGATTGTTGTCTCCACAAAGAGCCCAAAGTCTGACAAGCAATATGTATGGCAAGGAGAGGTGAATGCTAGCTCTTATACAATTCAGGAGGAGACTGATCCCGAGAAGCTTATTCCCAGAGGAACCCGCATCACCTTGTATCTCAAGCGCGATGACAAAGGTTTTGCTCATCCAGAACGGATTGAAAAGCTTGTGAAAAACTATTCACAATTTGTTTCCTTTCCTATATACACTTGGAAGGAAAAAGGATTCACTAAAGAGGTTGAGGTAGATGAAGATCCAGCTGAATCCAAAACGGATGAACAAGATGAGAAAACCGAGAAAAAGAAGAAGACGAAGACTGTTGTTGAGAAATACTGGGATTGGGATCTCACTAATGAGACACAACCAATATGGCTTCGCAATCCTAAGGAAGTTAATACAGAGGATTACAATGAGTTCTACAAGAAGACTTTCAATGAATACTTGGATCCACTAGCATCTTCGCACTTCACAACAGAGGGTGAAGTAGAGTTCAGGTCTATTCTATATGTGCCAGCTGTCCCTCCTATGGGGAAAGATGACATAGTCAATCCCAAGACGAAAAATATAAGGCTCTATGTGAAACGGGTGTTCATTTCAGATGACTTTGATGGAGAACTGTTCCCACGATATTTAAGCTTCGTCAAAGGTGTTGTGGACTCAAATGATCTTCCACTCAATGTCTCACGTGAAATTCTTCAGGAGAGTCGTATTGTACGGATTATGAGGAAGCGCTTGGTTCGGAAGGCATTTGACATGATCCTTGGAATATCAATGAGTGAGAACAGAGAAGATTATGAAAAGTTCTGGGAAAATTTTGGCAAACACTTGAAACTTGGTTGCATTGAAGATCGTGAAAATCATAAGCGTATTGCTCCGTTGCTTAGATTTTTCTCTTCCCAAAGTGAGGATGTGATGATCAGCTTGGATGAATATCTTGAAAACATGAAACCTGAGCAGAAGGATATCTATTATGTTGCTTCTGACAGCGTGACAAGTGCAAGCAATACACCATTCCTGGAGAAACTTCTTGAGAAGGACCTCGAAGTGTTGTACTTAGTTGATCCCATTGATGAGGTTGCCATCCAGAACCTAAAATCATACAAGGAGAAGAATTTCATTGATATTAGCAAGGAAGATCTCGATTTAGGGGATAAGAATGAGGAAAAAGAAAAGGAGATTAAGCAGGAATATGGCCAAACATGTGATTGGATTAAGAAGCGTTTGGGTGACAAAGTTGCCAGTGTTCAGATTTCAAACCGTCTTAGCTCGTCACCCTGTGTTCTTGTGTCTGGGAAGTTTGGCTGGTCTGCCAACATGGAGAGATTGATGAAGGCACAAACTGTTGGTGATACCTCTAGCCTGGAGTATATGAGAGGCAGGAGGGTGTTTGAGATTAATCCCGAGCACCCAATCATTAAAAACTTAAATGCTGCAAGCAAGACCAACCCGGATGATGAGGATGCTATAAGAGCTATTGATCTTCTGTACAATACAGCTTTGGTATCAAGCGGTTTTACTCCTGAAAATCCAGCAGAGCTGGGTGGAAAGATATACGAGATGATGAGTCTGGCACTCTCTGGGAAATGGTCCACACCTGCTGCCGAAATTCAGCAACCCGAACTCCAGCAACACAACCCAGAGATACTAGACGCCGAGGTACAGCACCCAGGACCCCAACACAACCACCCCGAGACGGTAGAGGCCGAGGTGGTTGAGCCCGTTGAAGCTGGCAGCCAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0008144 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0017076 GO:0030312 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04141 ko04151 ko04626 ko04657 ko04915 ko04918 ko05200 ko05215 ko05418 map04141 map04151 map04626 map04657 map04915 map04918 map05200 map05215 map05418

Pfam Domains

Protein Families

HATPase_c HSP90

Protein Analysis

810

Amino Acids

91.89

Weight (kDa)

5.29

Isoelectric Point (pI)

37.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HATPase_c_3	PF13589	113 - 244	2.1e-07	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
HATPase_c	PF02518	113 - 271	1.1e-06	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
HSP90	PF00183	276 - 764	5.7e-192	Hsp90 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009264)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07770 AT3G07770 AT3G07770
fragaria_vesca	FvH4_6g41100 FvH4_6g41100
malus_domestica	MD09G1122200.v1.1 MD17G1113200.v1.1
prunus_persica	Prupe.3G201400_v2.0.a1
pyrus_communis	pycom09g04650 pycom17g10480
rosa_chinensis	RchiOBHm_Chr2g0156661
rosa_laevigata	RLG00000020929
rosa_multiflora	Rmu_sc0001130.1_g000001 Rmu_sc0018228.1_g000001
rosa_roxburghii	Rroxscaffold_2G00092860
rosa_rugosa	Rorug02G0455200
rosa_samantha	Rh2AG520800 Rh2BG534000 Rh2CG505800 Rh2DG542800
rosa_wichuraiana	Rw0G013720 Rw2G042970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2290
Acc36I	ACCTGC	1 cut(s)	2290
AccII	CGCG	2 cut(s)	263, 813
AciI	CCGC	9 cut(s)	30, 48, 71, 102, 242, 261, 263, 790, 2196
AclWI	GGATC	7 cut(s)	755, 935, 1038, 1127, 1140, 1438, 1742
AcoI	YGGCCR	1 cut(s)	1879
AcsI	RAATTY	4 cut(s)	1377, 1498, 1798, 2292
AcuI	CTGAAG	1 cut(s)	1367
AcvI	CACGTG	1 cut(s)	1373
AcyI	GRCGYC	1 cut(s)	2337
AdeI	CACNNNGTG	3 cut(s)	145, 551, 1964
AfaI	GTAC	5 cut(s)	326, 1401, 1739, 2174, 2346
AfeI	AGCGCT	1 cut(s)	1418
AfiI	CCNNNNNNNGG	5 cut(s)	49, 1213, 1214, 1591, 2380
AflIII	ACRYGT	1 cut(s)	1886
AhdI	GACNNNNNGTC	1 cut(s)	23
AjnI	CCWGG	4 cut(s)	154, 1701, 2046, 2353
Alw21I	GWGCWC	1 cut(s)	2094
Alw26I	GTCTC	9 cut(s)	31, 110, 260, 302, 679, 749, 1037, 1372, 2372
AlwI	GGATC	7 cut(s)	755, 935, 1038, 1127, 1140, 1438, 1742
Ama87I	CYCGRG	3 cut(s)	764, 2087, 2375
Aor51HI	AGCGCT	1 cut(s)	1418
AoxI	GGCC	3 cut(s)	243, 1879, 2388
ApeKI	GCWGC	4 cut(s)	239, 2117, 2285, 2418
ApoI	RAATTY	4 cut(s)	1377, 1498, 1798, 2292
ArsI	GACNNNNNNTTYG	2 cut(s)	809, 841
AseI	ATTAAT	1 cut(s)	2082
Asp700I	GAANNNNTTC	4 cut(s)	342, 1115, 1380, 1799
AspLEI	GCGC	4 cut(s)	42, 89, 813, 1419
AspS9I	GGNCC	5 cut(s)	151, 304, 1723, 2274, 2357
AsuC2I	CCSGG	1 cut(s)	2135
AsuHPI	GGTGA	7 cut(s)	446, 736, 787, 1178, 1925, 1950, 2045
AsuNHI	GCTAGC	1 cut(s)	731
AvaI	CYCGRG	3 cut(s)	764, 2087, 2375
AvaII	GGWCC	5 cut(s)	151, 304, 1723, 2274, 2357
AxyI	CCTNAGG	1 cut(s)	1071
BaeGI	GKGCMC	1 cut(s)	55
BalI	TGGCCA	1 cut(s)	1881
BamHI	GGATCC	1 cut(s)	1132
BanII	GRGCYC	2 cut(s)	689, 2406
BbrPI	CACGTG	1 cut(s)	1373
BbsI	GAAGAC	3 cut(s)	1001, 1007, 1118
Bbv12I	GWGCWC	1 cut(s)	2094
BbvCI	CCTCAGC	1 cut(s)	285
BbvI	GCAGC	3 cut(s)	226, 2104, 2272
BccI	CCATC	5 cut(s)	41, 116, 129, 1289, 1775
BceAI	ACGGC	2 cut(s)	61, 252
BciT130I	CCWGG	4 cut(s)	156, 1703, 2048, 2355
BciVI	GTATCC	1 cut(s)	461
BclI	TGATCA	1 cut(s)	1602
BcnI	CCSGG	1 cut(s)	2135
BcoDI	GTCTC	9 cut(s)	31, 110, 260, 302, 679, 749, 1037, 1372, 2372
BfaI	CTAG	5 cut(s)	440, 732, 1139, 2043, 2333
BfoI	RGCGCY	1 cut(s)	1420
BfuAI	ACCTGC	1 cut(s)	2290
BfuI	GTATCC	1 cut(s)	461
BglII	AGATCT	1 cut(s)	1822
BisI	GCNGC	7 cut(s)	72, 103, 240, 243, 2118, 2286, 2419
BlsI	GCNGC	7 cut(s)	73, 104, 241, 244, 2119, 2287, 2420
Bme1390I	CCNGG	5 cut(s)	156, 1703, 2048, 2135, 2355
Bme18I	GGWCC	5 cut(s)	151, 304, 1723, 2274, 2357
BmeRI	GACNNNNNGTC	1 cut(s)	23
BmeT110I	CYCGRG	3 cut(s)	764, 2087, 2375
BmgT120I	GGNCC	5 cut(s)	151, 304, 1723, 2274, 2357
BmiI	GGNNCC	5 cut(s)	11, 153, 787, 1134, 2359
BmrFI	CCNGG	5 cut(s)	156, 1703, 2048, 2135, 2355
BmrI	ACTGGG	1 cut(s)	1030
BmsI	GCATC	6 cut(s)	241, 364, 583, 801, 1151, 2137
BmtI	GCTAGC	1 cut(s)	735
BmuI	ACTGGG	1 cut(s)	1030
BpiI	GAAGAC	3 cut(s)	1001, 1007, 1118
BpmI	CTGGAG	3 cut(s)	1724, 2069, 2294
Bpu10I	CCTNAGC	3 cut(s)	285, 414, 1638
BpuEI	CTTGAG	2 cut(s)	791, 1736
BpuMI	CCSGG	1 cut(s)	2135
BsaAI	YACGTR	1 cut(s)	1373
BsaBI	GATNNNNATC	4 cut(s)	609, 939, 1029, 1617
BsaHI	GRCGYC	1 cut(s)	2337
BsaJI	CCNNGG	9 cut(s)	43, 154, 155, 234, 261, 1447, 2340, 2353, 2391
BsaWI	WCCGGW	1 cut(s)	60
BsaXI	ACNNNNNCTCC	2 cut(s)	2292, 2322
Bsc4I	CCNNNNNNNGG	5 cut(s)	49, 1213, 1214, 1591, 2380
Bse1I	ACTGG	2 cut(s)	1025, 1926
Bse21I	CCTNAGG	1 cut(s)	1071
Bse3DI	GCAATG	1 cut(s)	370
Bse8I	GATNNNNATC	4 cut(s)	609, 939, 1029, 1617
BseBI	CCWGG	4 cut(s)	156, 1703, 2048, 2355
BseDI	CCNNGG	9 cut(s)	43, 154, 155, 234, 261, 1447, 2340, 2353, 2391
BseGI	GGATG	8 cut(s)	33, 832, 967, 1600, 1618, 1767, 2143, 2152
BseJI	GATNNNNATC	4 cut(s)	609, 939, 1029, 1617
BseLI	CCNNNNNNNGG	5 cut(s)	49, 1213, 1214, 1591, 2380
BseMI	GCAATG	1 cut(s)	370
BseMII	CTCAG	6 cut(s)	200, 276, 405, 412, 657, 1629
BseNI	ACTGG	2 cut(s)	1025, 1926
BseRI	GAGGAG	1 cut(s)	767
BseSI	GKGCMC	1 cut(s)	55
BseXI	GCAGC	3 cut(s)	226, 2104, 2272
BseYI	CCCAGC	1 cut(s)	2224
Bsh1236I	CGCG	2 cut(s)	263, 813
BshFI	GGCC	3 cut(s)	245, 1881, 2390
BsiHKAI	GWGCWC	1 cut(s)	2094
BsiHKCI	CYCGRG	3 cut(s)	764, 2087, 2375
BsiSI	CCGG	2 cut(s)	61, 2135
BslFI	GGGAC	3 cut(s)	137, 568, 1190
BslI	CCNNNNNNNGG	5 cut(s)	49, 1213, 1214, 1591, 2380
BsmAI	GTCTC	9 cut(s)	31, 110, 260, 302, 679, 749, 1037, 1372, 2372
BsmBI	CGTCTC	3 cut(s)	31, 302, 2372
BsmFI	GGGAC	3 cut(s)	137, 568, 1190
BsmI	GAATGC	2 cut(s)	647, 733
BsnI	GGCC	3 cut(s)	245, 1881, 2390
BsoBI	CYCGRG	3 cut(s)	764, 2087, 2375
Bsp1286I	GDGCHC	4 cut(s)	55, 689, 2094, 2406
Bsp1407I	TGTACA	2 cut(s)	324, 2172
BspACI	CCGC	9 cut(s)	30, 48, 71, 102, 242, 261, 263, 790, 2196
BspANI	GGCC	3 cut(s)	245, 1881, 2390
BspCNI	CTCAG	6 cut(s)	199, 277, 406, 411, 656, 1630
BspFNI	CGCG	2 cut(s)	263, 813
BspLI	GGNNCC	5 cut(s)	11, 153, 787, 1134, 2359
BspMI	ACCTGC	1 cut(s)	2290
BspOI	GCTAGC	1 cut(s)	735
BspPI	GGATC	7 cut(s)	755, 935, 1038, 1127, 1140, 1438, 1742
BsrDI	GCAATG	1 cut(s)	370
BsrGI	TGTACA	2 cut(s)	324, 2172
BsrI	ACTGG	2 cut(s)	1025, 1926
BssECI	CCNNGG	9 cut(s)	43, 154, 155, 234, 261, 1447, 2340, 2353, 2391
BssNI	GRCGYC	1 cut(s)	2337
BssT1I	CCWWGG	1 cut(s)	1447
Bst2UI	CCWGG	4 cut(s)	156, 1703, 2048, 2355
Bst4CI	ACNGT	7 cut(s)	329, 530, 1006, 1305, 1946, 2029, 2383
Bst6I	CTCTTC	1 cut(s)	1585
BstACI	GRCGYC	1 cut(s)	2337
BstAPI	GCANNNNNTGC	1 cut(s)	374
BstAUI	TGTACA	2 cut(s)	324, 2172
BstBAI	YACGTR	1 cut(s)	1373
BstC8I	GCNNGC	6 cut(s)	598, 733, 1200, 1687, 2122, 2416
BstDSI	CCRYGG	3 cut(s)	43, 234, 261
BstF5I	GGATG	8 cut(s)	33, 832, 967, 1600, 1618, 1767, 2143, 2152
BstFNI	CGCG	2 cut(s)	263, 813
BstH2I	RGCGCY	1 cut(s)	1420
BstHHI	GCGC	4 cut(s)	42, 89, 813, 1419
BstMAI	GTCTC	9 cut(s)	31, 110, 260, 302, 679, 749, 1037, 1372, 2372
BstMWI	GCNNNNNNNGC	4 cut(s)	251, 260, 374, 2156
BstNI	CCWGG	4 cut(s)	156, 1703, 2048, 2355
BstNSI	RCATGY	2 cut(s)	211, 1890
BstSCI	CCNGG	5 cut(s)	154, 1701, 2046, 2133, 2353
BstSLI	GKGCMC	1 cut(s)	55
BstUI	CGCG	2 cut(s)	263, 813
BstV1I	GCAGC	3 cut(s)	226, 2104, 2272
BstV2I	GAAGAC	3 cut(s)	1001, 1007, 1118
BstX2I	RGATCY	4 cut(s)	940, 1030, 1132, 1822
BstYI	RGATCY	4 cut(s)	940, 1030, 1132, 1822
Bsu36I	CCTNAGG	1 cut(s)	1071
BsuI	GTATCC	1 cut(s)	461
BsuRI	GGCC	3 cut(s)	245, 1881, 2390
BtgI	CCRYGG	3 cut(s)	43, 234, 261
BtgZI	GCGATG	1 cut(s)	828
BtsCI	GGATG	8 cut(s)	33, 832, 967, 1600, 1618, 1767, 2143, 2152
BtsI	GCAGTG	2 cut(s)	97, 607
BtsIMutI	CAGTG	4 cut(s)	97, 406, 607, 1933
BveI	ACCTGC	1 cut(s)	2290
Cac8I	GCNNGC	6 cut(s)	598, 733, 1200, 1687, 2122, 2416
CfoI	GCGC	4 cut(s)	42, 89, 813, 1419
Cfr13I	GGNCC	5 cut(s)	151, 304, 1723, 2274, 2357
Cfr42I	CCGCGG	1 cut(s)	264
CseI	GACGC	2 cut(s)	27, 2345
Csp6I	GTAC	5 cut(s)	325, 1400, 1738, 2173, 2345
CviAII	CATG	6 cut(s)	208, 301, 1441, 1630, 1887, 2002
CviQI	GTAC	5 cut(s)	325, 1400, 1738, 2173, 2345
DraI	TTTAAA	1 cut(s)	198
DraIII	CACNNNGTG	3 cut(s)	145, 551, 1964
DriI	GACNNNNNGTC	1 cut(s)	23
EaeI	YGGCCR	1 cut(s)	1879
Eam1104I	CTCTTC	1 cut(s)	1585
Eam1105I	GACNNNNNGTC	1 cut(s)	23
EarI	CTCTTC	1 cut(s)	1585
EciI	GGCGGA	1 cut(s)	19
Eco130I	CCWWGG	1 cut(s)	1447
Eco24I	GRGCYC	2 cut(s)	689, 2406
Eco32I	GATATC	2 cut(s)	445, 1651
Eco47I	GGWCC	5 cut(s)	151, 304, 1723, 2274, 2357
Eco47III	AGCGCT	1 cut(s)	1418
Eco57I	CTGAAG	1 cut(s)	1367
Eco72I	CACGTG	1 cut(s)	1373
Eco81I	CCTNAGG	1 cut(s)	1071
Eco88I	CYCGRG	3 cut(s)	764, 2087, 2375
EcoO109I	RGGNCCY	2 cut(s)	1723, 2357
EcoRII	CCWGG	4 cut(s)	154, 1701, 2046, 2353
EcoRV	GATATC	2 cut(s)	445, 1651
EcoT14I	CCWWGG	1 cut(s)	1447
EcoT22I	ATGCAT	1 cut(s)	379
EcoT38I	GRGCYC	2 cut(s)	689, 2406
ErhI	CCWWGG	1 cut(s)	1447
Esp3I	CGTCTC	3 cut(s)	31, 302, 2372
FaeI	CATG	6 cut(s)	211, 304, 1444, 1633, 1890, 2005
FalI	AAGNNNNNCTT	4 cut(s)	330, 362, 541, 573
FaqI	GGGAC	3 cut(s)	137, 568, 1190
FatI	CATG	6 cut(s)	207, 300, 1440, 1629, 1886, 2001
FauI	CCCGC	3 cut(s)	41, 268, 797
FbaI	TGATCA	1 cut(s)	1602
Fnu4HI	GCNGC	7 cut(s)	72, 103, 240, 243, 2118, 2286, 2419
FokI	GGATG	8 cut(s)	20, 819, 974, 1607, 1625, 1754, 2150, 2159
FriOI	GRGCYC	2 cut(s)	689, 2406
Fsp4HI	GCNGC	7 cut(s)	72, 103, 240, 243, 2118, 2286, 2419
FspBI	CTAG	5 cut(s)	440, 732, 1139, 2043, 2333
GlaI	GCGC	4 cut(s)	41, 88, 812, 1418
GluI	GCNGC	7 cut(s)	72, 103, 240, 243, 2118, 2286, 2419
GsaI	CCCAGC	1 cut(s)	2228
GsuI	CTGGAG	3 cut(s)	1724, 2069, 2294
HaeII	RGCGCY	1 cut(s)	1420
HaeIII	GGCC	3 cut(s)	245, 1881, 2390
HapII	CCGG	2 cut(s)	61, 2135
HgaI	GACGC	2 cut(s)	27, 2345
HhaI	GCGC	4 cut(s)	42, 89, 813, 1419
Hin1I	GRCGYC	1 cut(s)	2337
Hin1II	CATG	6 cut(s)	211, 304, 1444, 1633, 1890, 2005
Hin6I	GCGC	4 cut(s)	40, 87, 811, 1417
HinP1I	GCGC	4 cut(s)	40, 87, 811, 1417
HincII	GTYRAC	1 cut(s)	316
HindII	GTYRAC	1 cut(s)	316
HindIII	AAGCTT	3 cut(s)	770, 851, 1321
HpaI	GTTAAC	1 cut(s)	316
HpaII	CCGG	2 cut(s)	61, 2135
HphI	GGTGA	7 cut(s)	446, 736, 787, 1178, 1925, 1950, 2045
Hpy166II	GTNNAC	4 cut(s)	234, 316, 1342, 2277
Hpy8I	GTNNAC	4 cut(s)	234, 316, 1342, 2277
Hpy99I	CGWCG	1 cut(s)	21
HpyAV	CCTTC	8 cut(s)	418, 565, 574, 895, 1423, 1639, 1714, 2011
HpyCH4III	ACNGT	7 cut(s)	329, 530, 1006, 1305, 1946, 2029, 2383
HpyCH4IV	ACGT	2 cut(s)	55, 1372
HpyCH4V	TGCA	9 cut(s)	4, 254, 368, 377, 545, 659, 1527, 1685, 2120
HpyF10VI	GCNNNNNNNGC	4 cut(s)	251, 260, 374, 2156
HpySE526I	ACGT	2 cut(s)	55, 1372
Hsp92I	GRCGYC	1 cut(s)	2337
Hsp92II	CATG	6 cut(s)	211, 304, 1444, 1633, 1890, 2005
HspAI	GCGC	4 cut(s)	40, 87, 811, 1417
Ksp22I	TGATCA	1 cut(s)	1602
KspAI	GTTAAC	1 cut(s)	316
KspI	CCGCGG	1 cut(s)	264
LmnI	GCTCC	4 cut(s)	15, 26, 393, 1564
Lsp1109I	GCAGC	3 cut(s)	226, 2104, 2272
LweI	GCATC	6 cut(s)	241, 364, 583, 801, 1151, 2137
MaeI	CTAG	5 cut(s)	440, 732, 1139, 2043, 2333
MaeII	ACGT	2 cut(s)	55, 1372
MaeIII	GTNAC	7 cut(s)	56, 164, 329, 403, 1675, 1913, 1956
MfeI	CAATTG	1 cut(s)	540
MflI	RGATCY	4 cut(s)	940, 1030, 1132, 1822
MhlI	GDGCHC	4 cut(s)	55, 689, 2094, 2406
MlsI	TGGCCA	1 cut(s)	1881
MluCI	AATT	9 cut(s)	175, 193, 540, 744, 872, 1377, 1498, 1798, 2292
MluNI	TGGCCA	1 cut(s)	1881
MlyI	GAGTC	6 cut(s)	178, 239, 502, 1337, 1399, 2260
Mox20I	TGGCCA	1 cut(s)	1881
Mph1103I	ATGCAT	1 cut(s)	379
MroXI	GAANNNNTTC	4 cut(s)	342, 1115, 1380, 1799
MscI	TGGCCA	1 cut(s)	1881
Msp20I	TGGCCA	1 cut(s)	1881
MspA1I	CMGCKG	3 cut(s)	263, 947, 1202
MspI	CCGG	2 cut(s)	61, 2135
MspR9I	CCNGG	5 cut(s)	156, 1703, 2048, 2135, 2355
MunI	CAATTG	1 cut(s)	540
Mva1269I	GAATGC	2 cut(s)	647, 733
MvaI	CCWGG	4 cut(s)	156, 1703, 2048, 2355
MvnI	CGCG	2 cut(s)	263, 813
MwoI	GCNNNNNNNGC	4 cut(s)	251, 260, 374, 2156
NciI	CCSGG	1 cut(s)	2135
NheI	GCTAGC	1 cut(s)	731
NlaIII	CATG	6 cut(s)	211, 304, 1444, 1633, 1890, 2005
NlaIV	GGNNCC	5 cut(s)	11, 153, 787, 1134, 2359
NmeAIII	GCCGAG	3 cut(s)	490, 2365, 2416
NmuCI	GTSAC	4 cut(s)	403, 1675, 1913, 1956
NsiI	ATGCAT	1 cut(s)	379
NspI	RCATGY	2 cut(s)	211, 1890
PaqCI	CACCTGC	1 cut(s)	2290
PasI	CCCWGGG	1 cut(s)	155
PciI	ACATGT	1 cut(s)	1886
PctI	GAATGC	2 cut(s)	647, 733
PdmI	GAANNNNTTC	4 cut(s)	342, 1115, 1380, 1799
PfeI	GAWTC	5 cut(s)	474, 491, 498, 912, 950
PflFI	GACNNNGTC	1 cut(s)	1229
PfoI	TCCNGGA	1 cut(s)	1701
PkrI	GCNGC	7 cut(s)	73, 104, 241, 244, 2119, 2287, 2420
PleI	GAGTC	6 cut(s)	178, 238, 502, 1337, 1398, 2259
PmaCI	CACGTG	1 cut(s)	1373
PmlI	CACGTG	1 cut(s)	1373
PpsI	GAGTC	6 cut(s)	178, 238, 502, 1337, 1398, 2259
Ppu21I	YACGTR	1 cut(s)	1373
PpuMI	RGGWCCY	2 cut(s)	1723, 2357
PscI	ACATGT	1 cut(s)	1886
PshBI	ATTAAT	1 cut(s)	2082
Psp5II	RGGWCCY	2 cut(s)	1723, 2357
Psp6I	CCWGG	4 cut(s)	154, 1701, 2046, 2353
PspCI	CACGTG	1 cut(s)	1373
PspFI	CCCAGC	1 cut(s)	2224
PspGI	CCWGG	4 cut(s)	154, 1701, 2046, 2353
PspN4I	GGNNCC	5 cut(s)	11, 153, 787, 1134, 2359
PspPI	GGNCC	5 cut(s)	151, 304, 1723, 2274, 2357
PspPPI	RGGWCCY	2 cut(s)	1723, 2357
PsuI	RGATCY	4 cut(s)	940, 1030, 1132, 1822
PsyI	GACNNNGTC	1 cut(s)	1229
PvuII	CAGCTG	2 cut(s)	947, 1202
RsaI	GTAC	5 cut(s)	326, 1401, 1739, 2174, 2346
RsaNI	GTAC	5 cut(s)	325, 1400, 1738, 2173, 2345
SacII	CCGCGG	1 cut(s)	264
SatI	GCNGC	7 cut(s)	72, 103, 240, 243, 2118, 2286, 2419
Sau96I	GGNCC	5 cut(s)	151, 304, 1723, 2274, 2357
SchI	GAGTC	6 cut(s)	178, 239, 502, 1337, 1399, 2260
ScrFI	CCNGG	5 cut(s)	156, 1703, 2048, 2135, 2355
SduI	GDGCHC	4 cut(s)	55, 689, 2094, 2406
SfaNI	GCATC	6 cut(s)	241, 364, 583, 801, 1151, 2137
Sfr303I	CCGCGG	1 cut(s)	264
SgrBI	CCGCGG	1 cut(s)	264
SinI	GGWCC	5 cut(s)	151, 304, 1723, 2274, 2357
SmlI	CTYRAG	2 cut(s)	806, 1715
SmoI	CTYRAG	2 cut(s)	806, 1715
Sse9I	AATT	9 cut(s)	175, 193, 540, 744, 872, 1377, 1498, 1798, 2292
SsiI	CCGC	9 cut(s)	30, 48, 71, 102, 242, 261, 263, 790, 2196
SspMI	CTAG	5 cut(s)	440, 732, 1139, 2043, 2333
StyD4I	CCNGG	5 cut(s)	154, 1701, 2046, 2133, 2353
StyI	CCWWGG	1 cut(s)	1447
TaaI	ACNGT	7 cut(s)	329, 530, 1006, 1305, 1946, 2029, 2383
TaiI	ACGT	2 cut(s)	58, 1375
TaqI	TCGA	3 cut(s)	349, 1728, 1827
TasI	AATT	9 cut(s)	175, 193, 540, 744, 872, 1377, 1498, 1798, 2292
TatI	WGTACW	3 cut(s)	324, 1737, 2172
TauI	GCSGC	3 cut(s)	74, 105, 245
TfiI	GAWTC	5 cut(s)	474, 491, 498, 912, 950
TscAI	CASTG	4 cut(s)	104, 406, 607, 1933
TseFI	GTSAC	4 cut(s)	403, 1675, 1913, 1956
TseI	GCWGC	4 cut(s)	239, 2117, 2285, 2418
Tsp45I	GTSAC	4 cut(s)	403, 1675, 1913, 1956
TspDTI	ATGAA	9 cut(s)	951, 978, 1137, 1267, 1497, 1629, 1646, 1792, 2030
TspGWI	ACGGA	8 cut(s)	13, 133, 380, 437, 856, 974, 1417, 1551
TspRI	CASTG	4 cut(s)	104, 406, 607, 1933
Tth111I	GACNNNGTC	1 cut(s)	1229
VpaK11BI	GGWCC	5 cut(s)	151, 304, 1723, 2274, 2357
VspI	ATTAAT	1 cut(s)	2082
XapI	RAATTY	4 cut(s)	1377, 1498, 1798, 2292
XbaI	TCTAGA	1 cut(s)	439
XceI	RCATGY	2 cut(s)	211, 1890
XmnI	GAANNNNTTC	4 cut(s)	342, 1115, 1380, 1799
XspI	CTAG	5 cut(s)	440, 732, 1139, 2043, 2333
Zsp2I	ATGCAT	1 cut(s)	379

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.