MD09G1127700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014123
Physical Location & Seq
Reverse (-)
9838112 .. 9838907
796 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1127700.v1.1.491

Sequence Viewer

Length: 753 bp
ATGGAGAGGCACATCGAGATATTCCTGAAGAGGCTCTCCTACGTCGCCATCCTCATCGGCACCGTCACCTTCGTCTTTCTCCTCCTCAAAAACCCCGACACCTGCGTCGCACCAGGTTCTCCCCCCAAGCCCCACCTCCGCTTCCCCAAATCCTCCTGCGACGCCTCGCCGCGCCTGATCCTCCCTCTCCACAAGAAGAACAACCGCCTCTGGTCCTCCAAGACCTGGCAGAACAAGGTCCGCTCCTTCTCCAACTTCTTTCGCGCATTTTATCAAATGGGTCTCCTGCACAACGAGTCCAAGGTCCTCTGCGCCTCTGCCGGCGCCGGCCACGAGGTCATGGCGCTGACCGAGTTGGGCGTCGCCGACGTCACCGGTATTGAGCTCCTCGAATCGCCTCCGCTGGTGAGCCGGGCCGATCCCCACAACTTGCCTTATTTCGATAATGTGTTTGATTTGGGTTTCAGTGCTCATTTTGCCGAGGCATTGTTTCCGAACCGGTTCGCGGCGGAGATGGAGAGGACGGTGAGGGCCGGTGGGGTGTGTGTGGTGGTTGTGGATGAATGTGGGGATGAGGAGATTAGGGAGATTGTGGATTTGTTTTCGAATTCGAGGTTTCTTGGTGCTCTTAATGTTACATTGACTGGATTGAGAATGACAAGGATTATTATGAAAGTGTTTATTGCATTTGTTTTCGATTTCGAATTCCTGTTGGTGTGTGAATTAGTAATTCTCTTTAGGATCTGGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

251

Amino Acids

28.23

Weight (kDa)

7.04

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 105 - 184 5.9e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014123)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G16030 AT4G26730
fragaria_vesca FvH4_6g43920 FvH4_6g43920
malus_domestica MD09G1102000.v1.1 MD09G1127700.v1.1
prunus_persica Prupe.3G223700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0160701
rosa_laevigata RLG00000021234
rosa_multiflora Rmu_co8474907.1_g000001
rosa_roxburghii Rroxscaffold_2G00089470
rosa_rugosa Rorug02G0485100
rosa_samantha Rh2AG550100 Rh2BG564600 Rh2CG534500 Rh2DG573400
rosa_wichuraiana Rw2G045580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 110
AasI GACNNNNNNGTC 1 cut(s) 104
AatII GACGTC 1 cut(s) 372
Acc36I ACCTGC 1 cut(s) 110
AccB1I GGYRCC 2 cut(s) 59, 323
AccB7I CCANNNNNTGG 1 cut(s) 225
AccBSI CCGCTC 1 cut(s) 243
AccII CGCG 3 cut(s) 172, 264, 506
AciI CCGC 7 cut(s) 139, 170, 205, 241, 401, 506, 509
AclWI GGATC 3 cut(s) 172, 413, 749
AcoI YGGCCR 1 cut(s) 328
AcsI RAATTY 2 cut(s) 607, 704
AcuI CTGAAG 1 cut(s) 47
AcyI GRCGYC 4 cut(s) 162, 324, 360, 369
AfiI CCNNNNNNNGG 2 cut(s) 225, 505
AgeI ACCGGT 2 cut(s) 374, 498
AjnI CCWGG 2 cut(s) 112, 224
AluBI AGCT 1 cut(s) 385
AluI AGCT 1 cut(s) 385
Alw21I GWGCWC 3 cut(s) 387, 472, 628
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 3 cut(s) 172, 413, 749
AoxI GGCC 3 cut(s) 328, 414, 531
ApoI RAATTY 2 cut(s) 607, 704
AsiGI ACCGGT 2 cut(s) 374, 498
Asp700I GAANNNNTTC 1 cut(s) 500
AspLEI GCGC 5 cut(s) 174, 266, 314, 326, 346
AspS9I GGNCC 5 cut(s) 213, 238, 304, 414, 531
AsuC2I CCSGG 1 cut(s) 413
AsuHPI GGTGA 4 cut(s) 58, 364, 418, 538
AsuII TTCGAA 2 cut(s) 605, 702
AvaII GGWCC 3 cut(s) 213, 238, 304
BanI GGYRCC 2 cut(s) 59, 323
BanII GRGCYC 1 cut(s) 387
BauI CACGAG 1 cut(s) 332
Bbv12I GWGCWC 3 cut(s) 387, 472, 628
BccI CCATC 2 cut(s) 56, 508
BciT130I CCWGG 2 cut(s) 114, 226
BcnI CCSGG 1 cut(s) 413
BcoDI GTCTC 1 cut(s) 287
BfoI RGCGCY 2 cut(s) 327, 347
BfuAI ACCTGC 1 cut(s) 110
BisI GCNGC 2 cut(s) 170, 507
BlsI GCNGC 2 cut(s) 171, 508
Bme1390I CCNGG 3 cut(s) 114, 226, 413
Bme18I GGWCC 3 cut(s) 213, 238, 304
BmgT120I GGNCC 5 cut(s) 213, 238, 304, 414, 531
BmiI GGNNCC 2 cut(s) 61, 325
BmrFI CCNGG 3 cut(s) 114, 226, 413
Bpu14I TTCGAA 2 cut(s) 605, 702
BpuMI CCSGG 1 cut(s) 413
BsaHI GRCGYC 4 cut(s) 162, 324, 360, 369
BsaI GGTCTC 1 cut(s) 287
BsaJI CCNNGG 2 cut(s) 300, 480
BsaWI WCCGGW 2 cut(s) 374, 498
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 505
Bse118I RCCGGY 5 cut(s) 320, 326, 374, 498, 533
Bse1I ACTGG 1 cut(s) 649
BseBI CCWGG 2 cut(s) 114, 226
BseDI CCNNGG 2 cut(s) 300, 480
BseGI GGATG 3 cut(s) 48, 565, 577
BseLI CCNNNNNNNGG 2 cut(s) 225, 505
BseNI ACTGG 1 cut(s) 649
BseRI GAGGAG 4 cut(s) 71, 74, 377, 590
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 3 cut(s) 172, 264, 506
BshFI GGCC 3 cut(s) 330, 416, 533
BshNI GGYRCC 2 cut(s) 59, 323
BshTI ACCGGT 2 cut(s) 374, 498
BsiHKAI GWGCWC 3 cut(s) 387, 472, 628
BsiSI CCGG 6 cut(s) 321, 327, 375, 412, 499, 534
BslI CCNNNNNNNGG 2 cut(s) 225, 505
BsmAI GTCTC 1 cut(s) 287
BsnI GGCC 3 cut(s) 330, 416, 533
Bso31I GGTCTC 1 cut(s) 287
Bsp119I TTCGAA 2 cut(s) 605, 702
Bsp1286I GDGCHC 3 cut(s) 387, 472, 628
Bsp143I GATC 3 cut(s) 177, 418, 741
BspACI CCGC 7 cut(s) 139, 170, 205, 241, 401, 506, 509
BspANI GGCC 3 cut(s) 330, 416, 533
BspFNI CGCG 3 cut(s) 172, 264, 506
BspLI GGNNCC 2 cut(s) 61, 325
BspMI ACCTGC 1 cut(s) 110
BspPI GGATC 3 cut(s) 172, 413, 749
BspT104I TTCGAA 2 cut(s) 605, 702
BspT107I GGYRCC 2 cut(s) 59, 323
BspTNI GGTCTC 1 cut(s) 287
BsrBI CCGCTC 1 cut(s) 243
BsrFI RCCGGY 5 cut(s) 320, 326, 374, 498, 533
BsrI ACTGG 1 cut(s) 649
BssAI RCCGGY 5 cut(s) 320, 326, 374, 498, 533
BssECI CCNNGG 2 cut(s) 300, 480
BssMI GATC 3 cut(s) 177, 418, 741
BssNI GRCGYC 4 cut(s) 162, 324, 360, 369
BssSI CACGAG 1 cut(s) 332
BssT1I CCWWGG 1 cut(s) 300
Bst2BI CACGAG 1 cut(s) 332
Bst2UI CCWGG 2 cut(s) 114, 226
Bst4CI ACNGT 2 cut(s) 64, 526
Bst6I CTCTTC 1 cut(s) 23
BstACI GRCGYC 4 cut(s) 162, 324, 360, 369
BstBI TTCGAA 2 cut(s) 605, 702
BstC8I GCNNGC 2 cut(s) 322, 328
BstF5I GGATG 3 cut(s) 48, 565, 577
BstFNI CGCG 3 cut(s) 172, 264, 506
BstH2I RGCGCY 2 cut(s) 327, 347
BstHHI GCGC 5 cut(s) 174, 266, 314, 326, 346
BstKTI GATC 3 cut(s) 180, 421, 744
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 3 cut(s) 177, 418, 741
BstMWI GCNNNNNNNGC 1 cut(s) 476
BstNI CCWGG 2 cut(s) 114, 226
BstSCI CCNGG 3 cut(s) 112, 224, 411
BstUI CGCG 3 cut(s) 172, 264, 506
BstX2I RGATCY 1 cut(s) 741
BstYI RGATCY 1 cut(s) 741
BsuRI GGCC 3 cut(s) 330, 416, 533
BtsCI GGATG 3 cut(s) 48, 565, 577
BtsIMutI CAGTG 1 cut(s) 472
BveI ACCTGC 1 cut(s) 110
Cac8I GCNNGC 2 cut(s) 322, 328
CfoI GCGC 5 cut(s) 174, 266, 314, 326, 346
Cfr10I RCCGGY 5 cut(s) 320, 326, 374, 498, 533
Cfr13I GGNCC 5 cut(s) 213, 238, 304, 414, 531
CseI GACGC 3 cut(s) 94, 170, 349
CsiI ACCWGGT 1 cut(s) 112
CspAI ACCGGT 2 cut(s) 374, 498
CviAII CATG 1 cut(s) 340
CviJI RGCY 7 cut(s) 34, 130, 330, 385, 411, 416, 533
CviKI_1 RGCY 7 cut(s) 34, 130, 330, 385, 411, 416, 533
DinI GGCGCC 1 cut(s) 325
DpnI GATC 3 cut(s) 179, 420, 743
DpnII GATC 3 cut(s) 177, 418, 741
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 1 cut(s) 328
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 1 cut(s) 524
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 387
Eco31I GGTCTC 1 cut(s) 287
Eco47I GGWCC 3 cut(s) 213, 238, 304
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 47
EcoICRI GAGCTC 1 cut(s) 385
EcoO109I RGGNCCY 1 cut(s) 304
EcoRI GAATTC 2 cut(s) 607, 704
EcoRII CCWGG 2 cut(s) 112, 224
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 387
EgeI GGCGCC 1 cut(s) 325
EheI GGCGCC 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 1 cut(s) 343
FaiI YATR 2 cut(s) 341, 671
FatI CATG 1 cut(s) 339
Fnu4HI GCNGC 2 cut(s) 170, 507
FokI GGATG 3 cut(s) 35, 572, 584
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 2 cut(s) 170, 507
GlaI GCGC 5 cut(s) 173, 265, 313, 325, 345
GluI GCNGC 2 cut(s) 170, 507
HaeII RGCGCY 2 cut(s) 327, 347
HaeIII GGCC 3 cut(s) 330, 416, 533
HapII CCGG 6 cut(s) 321, 327, 375, 412, 499, 534
HgaI GACGC 3 cut(s) 94, 170, 349
HhaI GCGC 5 cut(s) 174, 266, 314, 326, 346
Hin1I GRCGYC 4 cut(s) 162, 324, 360, 369
Hin1II CATG 1 cut(s) 343
Hin6I GCGC 5 cut(s) 172, 264, 312, 324, 344
HinP1I GCGC 5 cut(s) 172, 264, 312, 324, 344
HinfI GANTC 2 cut(s) 296, 392
HpaII CCGG 6 cut(s) 321, 327, 375, 412, 499, 534
HphI GGTGA 4 cut(s) 58, 364, 418, 538
Hpy188I TCNGA 1 cut(s) 495
Hpy188III TCNNGA 2 cut(s) 16, 25
Hpy99I CGWCG 5 cut(s) 47, 110, 164, 365, 371
HpyAV CCTTC 2 cut(s) 79, 256
HpyCH4III ACNGT 2 cut(s) 64, 526
HpyCH4IV ACGT 2 cut(s) 42, 369
HpyCH4V TGCA 2 cut(s) 289, 686
HpyF10VI GCNNNNNNNGC 1 cut(s) 476
HpySE526I ACGT 2 cut(s) 42, 369
Hsp92I GRCGYC 4 cut(s) 162, 324, 360, 369
Hsp92II CATG 1 cut(s) 343
HspAI GCGC 5 cut(s) 172, 264, 312, 324, 344
KasI GGCGCC 1 cut(s) 323
KroI GCCGGC 2 cut(s) 320, 326
KroNI GCCGGC 2 cut(s) 322, 328
Kzo9I GATC 3 cut(s) 177, 418, 741
LmnI GCTCC 2 cut(s) 248, 390
MabI ACCWGGT 1 cut(s) 112
MaeII ACGT 2 cut(s) 42, 369
MaeIII GTNAC 3 cut(s) 64, 370, 634
MalI GATC 3 cut(s) 179, 420, 743
MbiI CCGCTC 1 cut(s) 243
MboI GATC 3 cut(s) 177, 418, 741
MboII GAAGA 2 cut(s) 40, 208
MflI RGATCY 1 cut(s) 741
MhlI GDGCHC 3 cut(s) 387, 472, 628
MluCI AATT 4 cut(s) 607, 704, 722, 729
Mly113I GGCGCC 1 cut(s) 324
MlyI GAGTC 1 cut(s) 305
MmeI TCCRAC 1 cut(s) 276
MroNI GCCGGC 2 cut(s) 320, 326
MroXI GAANNNNTTC 1 cut(s) 500
MseI TTAA 1 cut(s) 630
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 6 cut(s) 321, 327, 375, 412, 499, 534
MspR9I CCNGG 3 cut(s) 114, 226, 413
MvaI CCWGG 2 cut(s) 114, 226
MvnI CGCG 3 cut(s) 172, 264, 506
MwoI GCNNNNNNNGC 1 cut(s) 476
NaeI GCCGGC 2 cut(s) 322, 328
NarI GGCGCC 1 cut(s) 324
NciI CCSGG 1 cut(s) 413
NdeII GATC 3 cut(s) 177, 418, 741
NgoMIV GCCGGC 2 cut(s) 320, 326
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 2 cut(s) 61, 325
NmeAIII GCCGAG 1 cut(s) 505
NmuCI GTSAC 2 cut(s) 64, 370
NspV TTCGAA 2 cut(s) 605, 702
PaqCI CACCTGC 1 cut(s) 110
PdiI GCCGGC 2 cut(s) 322, 328
PdmI GAANNNNTTC 1 cut(s) 500
PfeI GAWTC 1 cut(s) 392
PflMI CCANNNNNTGG 1 cut(s) 225
PinAI ACCGGT 2 cut(s) 374, 498
PkrI GCNGC 2 cut(s) 171, 508
PleI GAGTC 1 cut(s) 304
PluTI GGCGCC 1 cut(s) 327
PpsI GAGTC 1 cut(s) 304
PpuMI RGGWCCY 1 cut(s) 304
Psp124BI GAGCTC 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 304
Psp6I CCWGG 2 cut(s) 112, 224
PspGI CCWGG 2 cut(s) 112, 224
PspN4I GGNNCC 2 cut(s) 61, 325
PspPI GGNCC 5 cut(s) 213, 238, 304, 414, 531
PspPPI RGGWCCY 1 cut(s) 304
PsuI RGATCY 1 cut(s) 741
SacI GAGCTC 1 cut(s) 387
SaqAI TTAA 1 cut(s) 630
SatI GCNGC 2 cut(s) 170, 507
Sau3AI GATC 3 cut(s) 177, 418, 741
Sau96I GGNCC 5 cut(s) 213, 238, 304, 414, 531
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 3 cut(s) 114, 226, 413
SduI GDGCHC 3 cut(s) 387, 472, 628
SexAI ACCWGGT 1 cut(s) 112
SfoI GGCGCC 1 cut(s) 325
SfuI TTCGAA 2 cut(s) 605, 702
SinI GGWCC 3 cut(s) 213, 238, 304
Sse9I AATT 4 cut(s) 607, 704, 722, 729
SsiI CCGC 7 cut(s) 139, 170, 205, 241, 401, 506, 509
SspDI GGCGCC 1 cut(s) 323
SstI GAGCTC 1 cut(s) 387
StyD4I CCNGG 3 cut(s) 112, 224, 411
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 2 cut(s) 64, 526
TaiI ACGT 2 cut(s) 45, 372
TaqI TCGA 7 cut(s) 15, 390, 441, 605, 611, 696, 702
TaqII GACCGA 1 cut(s) 365
TasI AATT 4 cut(s) 607, 704, 722, 729
TauI GCSGC 2 cut(s) 172, 509
TfiI GAWTC 1 cut(s) 392
Tru1I TTAA 1 cut(s) 630
Tru9I TTAA 1 cut(s) 630
TscAI CASTG 1 cut(s) 472
TseFI GTSAC 2 cut(s) 64, 370
Tsp45I GTSAC 2 cut(s) 64, 370
TspDTI ATGAA 2 cut(s) 576, 686
TspRI CASTG 1 cut(s) 472
Van91I CCANNNNNTGG 1 cut(s) 225
VpaK11BI GGWCC 3 cut(s) 213, 238, 304
XapI RAATTY 2 cut(s) 607, 704
XmnI GAANNNNTTC 1 cut(s) 500
ZraI GACGTC 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.