MD09G1143500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014460
Physical Location & Seq
Reverse (-)
11183238 .. 11184338
1101 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1143500.v1.1.491

Sequence Viewer

Length: 603 bp
ATGGACGTAGAAGAAGAAACATATTATCTCACTTCACTCAGACAGCAAGAAGAAGAAGAAGAGAGAGAGAGTTTTTTAGCTTTGAGCTGCGCTTGCAAAGTGGTGGAGTATTTGCAGCCAGTGATGTCAAACGAACTGCTCTGCAAGTTCCCGGACAACTCCGCCTTTGATTTCGACTACTCGCAGAGCTCAATATGGTCCCCGTTGGTCCCCCGGGCTTATGCTCCCATGGATTTGGACCTGGATTTGGATTTCGCGACGCCGCGTAAGCTCAATTTTGAGATGGGGTTGGAAGTGAAAAATCAGAACAGCCTCGTCGAAGCGGGTTCCGGCATTAAGAAGAAGAAGATCCCCACAGCCACAACTTGTTTCAATCTGAATCGGAGTGCTCTGAAGAACAAGCTGATGAAGAGGAAGAAAAGCAAGATGGTCTTGGCCTCTGATTTCTCTCCGACTCCTGTTAACGTTAACTGTAACCCCATAGCCTCGAAGGTGTGGAATAAGGTGCTGAAAGCTGCCTCTAAGCATTTCAAGAAAAAGAAGAGAGATCCAATGGATCATGTGAGGTTCTCCAATTATTTGAGACCAGATGGAAATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

22.9

Weight (kDa)

8.86

Isoelectric Point (pI)

50.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15140
fragaria_vesca FvH4_6g39300
malus_domestica MD09G1143500.v1.1 MD17G1129800.v1.1
prunus_persica Prupe.3G184300_v2.0.a1
pyrus_communis pycom09g06300 pycom17g12240
rosa_chinensis RchiOBHm_Chr2g0153221 RchiOBHm_Chr3g0480451
rosa_multiflora Rmu_sc0000442.1_g000003
rosa_roxburghii Rroxscaffold_2G00095090 Rroxscaffold_5G00335020
rosa_rugosa Rorug02G0437600
rosa_samantha Rh2BG512100 Rh2CG486800 Rh4CG218900
rosa_wichuraiana Rw2G041080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 257, 265
AciI CCGC 3 cut(s) 162, 263, 323
AclI AACGTT 1 cut(s) 465
AclWI GGATC 3 cut(s) 343, 542, 564
AcuI CTGAAG 1 cut(s) 413
AcyI GRCGYC 1 cut(s) 260
AfiI CCNNNNNNNGG 1 cut(s) 247
AgsI TTSAA 2 cut(s) 373, 532
AjnI CCWGG 1 cut(s) 240
AluBI AGCT 6 cut(s) 80, 87, 189, 271, 403, 515
AluI AGCT 6 cut(s) 80, 87, 189, 271, 403, 515
Alw21I GWGCWC 2 cut(s) 191, 391
Alw26I GTCTC 1 cut(s) 577
AlwI GGATC 3 cut(s) 343, 542, 564
Ama87I CYCGRG 1 cut(s) 213
AoxI GGCC 1 cut(s) 435
ApeKI GCWGC 3 cut(s) 87, 115, 515
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 3 cut(s) 198, 208, 238
AsuC2I CCSGG 3 cut(s) 152, 214, 215
AvaI CYCGRG 1 cut(s) 213
AvaII GGWCC 3 cut(s) 198, 208, 238
BanII GRGCYC 1 cut(s) 191
Bbv12I GWGCWC 2 cut(s) 191, 391
BbvI GCAGC 3 cut(s) 74, 127, 502
BccI CCATC 3 cut(s) 277, 421, 584
BciT130I CCWGG 1 cut(s) 242
BcnI CCSGG 3 cut(s) 152, 214, 215
BcoDI GTCTC 1 cut(s) 577
BisI GCNGC 4 cut(s) 88, 116, 263, 516
BlsI GCNGC 4 cut(s) 89, 117, 264, 517
Bme1390I CCNGG 4 cut(s) 152, 214, 215, 242
Bme18I GGWCC 3 cut(s) 198, 208, 238
BmeT110I CYCGRG 1 cut(s) 213
BmgT120I GGNCC 3 cut(s) 198, 208, 238
BmiI GGNNCC 3 cut(s) 200, 210, 328
BmrFI CCNGG 4 cut(s) 152, 214, 215, 242
BpuMI CCSGG 3 cut(s) 152, 214, 215
BsaHI GRCGYC 1 cut(s) 260
BsaI GGTCTC 1 cut(s) 577
BsaJI CCNNGG 3 cut(s) 212, 213, 228
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse1I ACTGG 1 cut(s) 119
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 3 cut(s) 212, 213, 228
BseLI CCNNNNNNNGG 1 cut(s) 247
BseMII CTCAG 1 cut(s) 52
BseNI ACTGG 1 cut(s) 119
BseXI GCAGC 3 cut(s) 74, 127, 502
Bsh1236I CGCG 2 cut(s) 257, 265
BshFI GGCC 1 cut(s) 437
BsiHKAI GWGCWC 2 cut(s) 191, 391
BsiHKCI CYCGRG 1 cut(s) 213
BsiSI CCGG 3 cut(s) 152, 214, 330
BslFI GGGAC 2 cut(s) 184, 194
BslI CCNNNNNNNGG 1 cut(s) 247
BsmAI GTCTC 1 cut(s) 577
BsmFI GGGAC 2 cut(s) 184, 194
BsnI GGCC 1 cut(s) 437
Bso31I GGTCTC 1 cut(s) 577
BsoBI CYCGRG 1 cut(s) 213
Bsp1286I GDGCHC 2 cut(s) 191, 391
Bsp143I GATC 3 cut(s) 348, 547, 556
Bsp19I CCATGG 1 cut(s) 228
Bsp68I TCGCGA 1 cut(s) 257
BspACI CCGC 3 cut(s) 162, 263, 323
BspANI GGCC 1 cut(s) 437
BspCNI CTCAG 1 cut(s) 51
BspFNI CGCG 2 cut(s) 257, 265
BspLI GGNNCC 3 cut(s) 200, 210, 328
BspPI GGATC 3 cut(s) 343, 542, 564
BspTNI GGTCTC 1 cut(s) 577
BsrI ACTGG 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 212, 213, 228
BssMI GATC 3 cut(s) 348, 547, 556
BssNI GRCGYC 1 cut(s) 260
BssT1I CCWWGG 1 cut(s) 228
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 1 cut(s) 473
Bst6I CTCTTC 3 cut(s) 54, 404, 536
BstACI GRCGYC 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 2 cut(s) 38, 522
BstDSI CCRYGG 1 cut(s) 228
BstFNI CGCG 2 cut(s) 257, 265
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 3 cut(s) 351, 550, 559
BstMAI GTCTC 1 cut(s) 577
BstMBI GATC 3 cut(s) 348, 547, 556
BstMWI GCNNNNNNNGC 2 cut(s) 93, 268
BstNI CCWGG 1 cut(s) 242
BstSCI CCNGG 4 cut(s) 150, 212, 213, 240
BstUI CGCG 2 cut(s) 257, 265
BstV1I GCAGC 3 cut(s) 74, 127, 502
BstX2I RGATCY 2 cut(s) 348, 547
BstXI CCANNNNNNTGG 1 cut(s) 235
BstYI RGATCY 2 cut(s) 348, 547
BsuRI GGCC 1 cut(s) 437
BtgI CCRYGG 1 cut(s) 228
BtsIMutI CAGTG 1 cut(s) 126
BtuMI TCGCGA 1 cut(s) 257
Cac8I GCNNGC 1 cut(s) 94
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 3 cut(s) 198, 208, 238
Cfr9I CCCGGG 1 cut(s) 213
CseI GACGC 1 cut(s) 268
CviAII CATG 2 cut(s) 229, 560
DdeI CTNAG 2 cut(s) 38, 522
DpnI GATC 3 cut(s) 350, 549, 558
DpnII GATC 3 cut(s) 348, 547, 556
Eam1104I CTCTTC 3 cut(s) 54, 404, 536
EarI CTCTTC 3 cut(s) 54, 404, 536
EciI GGCGGA 1 cut(s) 151
Ecl136II GAGCTC 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 191
Eco31I GGTCTC 1 cut(s) 577
Eco47I GGWCC 3 cut(s) 198, 208, 238
Eco53kI GAGCTC 1 cut(s) 189
Eco57I CTGAAG 1 cut(s) 413
Eco88I CYCGRG 1 cut(s) 213
EcoICRI GAGCTC 1 cut(s) 189
EcoRII CCWGG 1 cut(s) 240
EcoT14I CCWWGG 1 cut(s) 228
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 2 cut(s) 232, 563
FaiI YATR 7 cut(s) 22, 196, 222, 230, 482, 561, 601
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 2 cut(s) 184, 194
FatI CATG 2 cut(s) 228, 559
FauI CCCGC 1 cut(s) 316
Fnu4HI GCNGC 4 cut(s) 88, 116, 263, 516
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 4 cut(s) 88, 116, 263, 516
GlaI GCGC 1 cut(s) 91
GluI GCNGC 4 cut(s) 88, 116, 263, 516
HaeIII GGCC 1 cut(s) 437
HapII CCGG 3 cut(s) 152, 214, 330
HgaI GACGC 1 cut(s) 268
HhaI GCGC 1 cut(s) 92
Hin1I GRCGYC 1 cut(s) 260
Hin1II CATG 2 cut(s) 232, 563
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 2 cut(s) 463, 469
HindII GTYRAC 2 cut(s) 463, 469
HinfI GANTC 2 cut(s) 379, 454
HpaI GTTAAC 2 cut(s) 463, 469
HpaII CCGG 3 cut(s) 152, 214, 330
Hpy166II GTNNAC 2 cut(s) 463, 469
Hpy188I TCNGA 7 cut(s) 41, 306, 378, 384, 393, 442, 453
Hpy188III TCNNGA 2 cut(s) 256, 532
Hpy8I GTNNAC 2 cut(s) 463, 469
Hpy99I CGWCG 2 cut(s) 262, 320
HpyAV CCTTC 1 cut(s) 484
HpyCH4III ACNGT 1 cut(s) 473
HpyCH4IV ACGT 2 cut(s) 6, 465
HpyCH4V TGCA 3 cut(s) 96, 115, 144
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 268
HpyF3I CTNAG 2 cut(s) 38, 522
HpySE526I ACGT 2 cut(s) 6, 465
Hsp92I GRCGYC 1 cut(s) 260
Hsp92II CATG 2 cut(s) 232, 563
HspAI GCGC 1 cut(s) 90
KspAI GTTAAC 2 cut(s) 463, 469
Kzo9I GATC 3 cut(s) 348, 547, 556
LmnI GCTCC 1 cut(s) 229
LpnPI CCDG 7 cut(s) 132, 165, 227, 227, 254, 343, 471
Lsp1109I GCAGC 3 cut(s) 74, 127, 502
MaeII ACGT 2 cut(s) 6, 465
MaeIII GTNAC 1 cut(s) 473
MalI GATC 3 cut(s) 350, 549, 558
MboI GATC 3 cut(s) 348, 547, 556
MflI RGATCY 2 cut(s) 348, 547
MhlI GDGCHC 2 cut(s) 191, 391
MluCI AATT 2 cut(s) 274, 574
MlyI GAGTC 1 cut(s) 448
MmeI TCCRAC 2 cut(s) 270, 476
MnlI CCTC 6 cut(s) 323, 405, 448, 496, 529, 558
MseI TTAA 3 cut(s) 336, 462, 468
MspI CCGG 3 cut(s) 152, 214, 330
MspR9I CCNGG 4 cut(s) 152, 214, 215, 242
MvaI CCWGG 1 cut(s) 242
MvnI CGCG 2 cut(s) 257, 265
MwoI GCNNNNNNNGC 2 cut(s) 93, 268
NciI CCSGG 3 cut(s) 152, 214, 215
NcoI CCATGG 1 cut(s) 228
NdeII GATC 3 cut(s) 348, 547, 556
NlaIII CATG 2 cut(s) 232, 563
NlaIV GGNNCC 3 cut(s) 200, 210, 328
NruI TCGCGA 1 cut(s) 257
PfeI GAWTC 1 cut(s) 379
PfoI TCCNGGA 1 cut(s) 150
PkrI GCNGC 4 cut(s) 89, 117, 264, 517
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
Psp124BI GAGCTC 1 cut(s) 191
Psp1406I AACGTT 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PspN4I GGNNCC 3 cut(s) 200, 210, 328
PspPI GGNCC 3 cut(s) 198, 208, 238
PsuI RGATCY 2 cut(s) 348, 547
RruI TCGCGA 1 cut(s) 257
SacI GAGCTC 1 cut(s) 191
SaqAI TTAA 3 cut(s) 336, 462, 468
SatI GCNGC 4 cut(s) 88, 116, 263, 516
Sau3AI GATC 3 cut(s) 348, 547, 556
Sau96I GGNCC 3 cut(s) 198, 208, 238
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 4 cut(s) 152, 214, 215, 242
SduI GDGCHC 2 cut(s) 191, 391
SinI GGWCC 3 cut(s) 198, 208, 238
SmaI CCCGGG 1 cut(s) 215
Sse9I AATT 2 cut(s) 274, 574
SsiI CCGC 3 cut(s) 162, 263, 323
SstI GAGCTC 1 cut(s) 191
StyD4I CCNGG 4 cut(s) 150, 212, 213, 240
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 1 cut(s) 473
TaiI ACGT 2 cut(s) 9, 468
TaqI TCGA 3 cut(s) 174, 318, 488
TasI AATT 2 cut(s) 274, 574
TauI GCSGC 1 cut(s) 265
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 3 cut(s) 336, 462, 468
Tru9I TTAA 3 cut(s) 336, 462, 468
TscAI CASTG 1 cut(s) 126
TseI GCWGC 3 cut(s) 87, 115, 515
TspDTI ATGAA 1 cut(s) 422
TspMI CCCGGG 1 cut(s) 213
TspRI CASTG 1 cut(s) 126
VpaK11BI GGWCC 3 cut(s) 198, 208, 238
XmaI CCCGGG 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.