pycom09g06300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0014460
Physical Location & Seq
Reverse (-)
4652885 .. 4653906
1022 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g06300.1

Sequence Viewer

Length: 777 bp
ATGTTTGGGCCTAGATCCGACATATCAGAATGTTTTTTGGTCAGACATCACATTGTTTCGGCCGTTGGACATTTTAAAATCTGCTCCAAAACCAAACTTCCCAACACAAAAGATCTTAGCTTTTGCGACCAAAAAACACCAAAGTTGTTAGCTTTTCACTTCGTTAATGGAAGTAGAAGAAGAAACATATTATCTCACTTCACTCAGACAGCAAGAAGAAGAAGAAGAGAGAGAGAGAGTTTTTTGGGTTTGAGCTGCGCTTGCAAAGTGGTGGAGTATTTGCAGCCAGTGATGTCAAACGAGCTGCTCTGCAAGTTCCCCGACAACTCCGCATTTGATTTCGACTACTCGCAGAGCTCGATATGGTCCCCGCTGGTCCCCCGGGCTTACGCTCCCGTGGATTTGGACCTGGATTTGGATTTGGATTTCGCGACGCCGCGTAAGCTCAATTTTGAGATGGGGTTGGAAGTGAAGAATCAGAACAGCCTTGTCGAAGCCGGTTCCGGCATTAAGAAGAAGAAGATCCCCACAACCACAACTTGTTTCAATCTGAATCGGAGTGCTCTGAAGAACAAGCTGATAAAGAGGAAGAAAAGCAAGATGGTTTTGGCCTCTGATTTCTCTCCGACTCCTGTTAACGTTAACTGTAACCCCATAGCCTCGAAGGTGTGGAATAAGGTGCTGAAAGCTGCCTCTAAGCATTTCAAGAAAAAGAAGAAAGATCCAATGGATCATGTGAGGTTCTTCAATTATTTGAGATCGGATGGAAATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

29.5

Weight (kDa)

9.91

Isoelectric Point (pI)

52.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15140
fragaria_vesca FvH4_6g39300
malus_domestica MD09G1143500.v1.1 MD17G1129800.v1.1
prunus_persica Prupe.3G184300_v2.0.a1
pyrus_communis pycom09g06300 pycom17g12240
rosa_chinensis RchiOBHm_Chr2g0153221 RchiOBHm_Chr3g0480451
rosa_multiflora Rmu_sc0000442.1_g000003
rosa_roxburghii Rroxscaffold_2G00095090 Rroxscaffold_5G00335020
rosa_rugosa Rorug02G0437600
rosa_samantha Rh2BG512100 Rh2CG486800 Rh4CG218900
rosa_wichuraiana Rw2G041080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 431, 439
AciI CCGC 3 cut(s) 330, 371, 437
AclI AACGTT 1 cut(s) 639
AclWI GGATC 4 cut(s) 9, 517, 716, 738
AcoI YGGCCR 1 cut(s) 60
AcuI CTGAAG 1 cut(s) 587
AcyI GRCGYC 1 cut(s) 434
AfiI CCNNNNNNNGG 1 cut(s) 415
AgsI TTSAA 3 cut(s) 547, 706, 748
AjnI CCWGG 1 cut(s) 408
AluBI AGCT 8 cut(s) 120, 152, 255, 304, 357, 445, 577, 689
AluI AGCT 8 cut(s) 120, 152, 255, 304, 357, 445, 577, 689
Alw21I GWGCWC 2 cut(s) 359, 565
AlwI GGATC 4 cut(s) 9, 517, 716, 738
Ama87I CYCGRG 1 cut(s) 381
AoxI GGCC 3 cut(s) 8, 60, 609
ApeKI GCWGC 4 cut(s) 255, 283, 304, 689
AspLEI GCGC 1 cut(s) 260
AspS9I GGNCC 4 cut(s) 8, 366, 376, 406
AsuC2I CCSGG 2 cut(s) 382, 383
AvaI CYCGRG 1 cut(s) 381
AvaII GGWCC 3 cut(s) 366, 376, 406
BanII GRGCYC 1 cut(s) 359
Bbv12I GWGCWC 2 cut(s) 359, 565
BbvI GCAGC 4 cut(s) 242, 291, 295, 676
BccI CCATC 3 cut(s) 451, 595, 758
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 1 cut(s) 410
BcnI CCSGG 2 cut(s) 382, 383
BfaI CTAG 1 cut(s) 12
BglII AGATCT 1 cut(s) 112
BisI GCNGC 5 cut(s) 256, 284, 305, 437, 690
BlsI GCNGC 5 cut(s) 257, 285, 306, 438, 691
Bme1390I CCNGG 3 cut(s) 382, 383, 410
Bme18I GGWCC 3 cut(s) 366, 376, 406
BmeT110I CYCGRG 1 cut(s) 381
BmgT120I GGNCC 4 cut(s) 8, 366, 376, 406
BmiI GGNNCC 3 cut(s) 368, 378, 502
BmrFI CCNGG 3 cut(s) 382, 383, 410
BpuMI CCSGG 2 cut(s) 382, 383
BsaHI GRCGYC 1 cut(s) 434
BsaJI CCNNGG 3 cut(s) 380, 381, 396
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 1 cut(s) 287
BseBI CCWGG 1 cut(s) 410
BseDI CCNNGG 3 cut(s) 380, 381, 396
BseGI GGATG 1 cut(s) 769
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 218
BseNI ACTGG 1 cut(s) 287
BseX3I CGGCCG 1 cut(s) 60
BseXI GCAGC 4 cut(s) 242, 291, 295, 676
Bsh1236I CGCG 2 cut(s) 431, 439
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 3 cut(s) 10, 62, 611
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 2 cut(s) 359, 565
BsiHKCI CYCGRG 1 cut(s) 381
BsiSI CCGG 3 cut(s) 382, 498, 504
BslFI GGGAC 2 cut(s) 352, 362
BslI CCNNNNNNNGG 1 cut(s) 415
BsmFI GGGAC 2 cut(s) 352, 362
BsnI GGCC 3 cut(s) 10, 62, 611
BsoBI CYCGRG 1 cut(s) 381
Bsp1286I GDGCHC 2 cut(s) 359, 565
Bsp143I GATC 6 cut(s) 14, 112, 522, 721, 730, 758
Bsp68I TCGCGA 1 cut(s) 431
BspACI CCGC 3 cut(s) 330, 371, 437
BspANI GGCC 3 cut(s) 10, 62, 611
BspCNI CTCAG 1 cut(s) 217
BspFNI CGCG 2 cut(s) 431, 439
BspLI GGNNCC 3 cut(s) 368, 378, 502
BspPI GGATC 4 cut(s) 9, 517, 716, 738
BsrFI RCCGGY 1 cut(s) 497
BsrI ACTGG 1 cut(s) 287
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 3 cut(s) 380, 381, 396
BssMI GATC 6 cut(s) 14, 112, 522, 721, 730, 758
BssNI GRCGYC 1 cut(s) 434
Bst2UI CCWGG 1 cut(s) 410
Bst4CI ACNGT 1 cut(s) 647
Bst6I CTCTTC 1 cut(s) 220
BstACI GRCGYC 1 cut(s) 434
BstC8I GCNNGC 1 cut(s) 262
BstDEI CTNAG 3 cut(s) 116, 204, 696
BstDSI CCRYGG 1 cut(s) 396
BstF5I GGATG 1 cut(s) 769
BstFNI CGCG 2 cut(s) 431, 439
BstHHI GCGC 1 cut(s) 260
BstKTI GATC 6 cut(s) 17, 115, 525, 724, 733, 761
BstMBI GATC 6 cut(s) 14, 112, 522, 721, 730, 758
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 2 cut(s) 261, 442
BstNI CCWGG 1 cut(s) 410
BstSCI CCNGG 3 cut(s) 380, 381, 408
BstUI CGCG 2 cut(s) 431, 439
BstV1I GCAGC 4 cut(s) 242, 291, 295, 676
BstX2I RGATCY 4 cut(s) 14, 112, 522, 721
BstYI RGATCY 4 cut(s) 14, 112, 522, 721
BstZI CGGCCG 1 cut(s) 60
BsuRI GGCC 3 cut(s) 10, 62, 611
BtgI CCRYGG 1 cut(s) 396
BtsCI GGATG 1 cut(s) 769
BtsIMutI CAGTG 1 cut(s) 294
BtuMI TCGCGA 1 cut(s) 431
Cac8I GCNNGC 1 cut(s) 262
CfoI GCGC 1 cut(s) 260
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 4 cut(s) 8, 366, 376, 406
Cfr9I CCCGGG 1 cut(s) 381
CseI GACGC 1 cut(s) 442
CviAII CATG 1 cut(s) 734
DdeI CTNAG 3 cut(s) 116, 204, 696
DpnI GATC 6 cut(s) 16, 114, 524, 723, 732, 760
DpnII GATC 6 cut(s) 14, 112, 522, 721, 730, 758
DraI TTTAAA 1 cut(s) 76
EaeI YGGCCR 1 cut(s) 60
EagI CGGCCG 1 cut(s) 60
Eam1104I CTCTTC 1 cut(s) 220
EarI CTCTTC 1 cut(s) 220
Ecl136II GAGCTC 1 cut(s) 357
EclXI CGGCCG 1 cut(s) 60
Eco24I GRGCYC 1 cut(s) 359
Eco47I GGWCC 3 cut(s) 366, 376, 406
Eco52I CGGCCG 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 357
Eco57I CTGAAG 1 cut(s) 587
Eco88I CYCGRG 1 cut(s) 381
EcoICRI GAGCTC 1 cut(s) 357
EcoRII CCWGG 1 cut(s) 408
EcoT38I GRGCYC 1 cut(s) 359
FaeI CATG 1 cut(s) 737
FaiI YATR 7 cut(s) 23, 188, 364, 656, 735, 773, 775
FaqI GGGAC 2 cut(s) 352, 362
FatI CATG 1 cut(s) 733
FauI CCCGC 1 cut(s) 378
Fnu4HI GCNGC 5 cut(s) 256, 284, 305, 437, 690
FriOI GRGCYC 1 cut(s) 359
Fsp4HI GCNGC 5 cut(s) 256, 284, 305, 437, 690
FspBI CTAG 1 cut(s) 12
GlaI GCGC 1 cut(s) 259
GluI GCNGC 5 cut(s) 256, 284, 305, 437, 690
HaeIII GGCC 3 cut(s) 10, 62, 611
HapII CCGG 3 cut(s) 382, 498, 504
HgaI GACGC 1 cut(s) 442
HhaI GCGC 1 cut(s) 260
Hin1I GRCGYC 1 cut(s) 434
Hin1II CATG 1 cut(s) 737
Hin6I GCGC 1 cut(s) 258
HinP1I GCGC 1 cut(s) 258
HincII GTYRAC 2 cut(s) 637, 643
HindII GTYRAC 2 cut(s) 637, 643
HinfI GANTC 3 cut(s) 475, 553, 628
HpaI GTTAAC 2 cut(s) 637, 643
HpaII CCGG 3 cut(s) 382, 498, 504
Hpy166II GTNNAC 2 cut(s) 637, 643
Hpy188III TCNNGA 2 cut(s) 430, 706
Hpy8I GTNNAC 2 cut(s) 637, 643
Hpy99I CGWCG 1 cut(s) 436
HpyAV CCTTC 1 cut(s) 658
HpyCH4III ACNGT 1 cut(s) 647
HpyCH4IV ACGT 1 cut(s) 639
HpyCH4V TGCA 3 cut(s) 264, 283, 312
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 442
HpyF3I CTNAG 3 cut(s) 116, 204, 696
HpySE526I ACGT 1 cut(s) 639
Hsp92I GRCGYC 1 cut(s) 434
Hsp92II CATG 1 cut(s) 737
HspAI GCGC 1 cut(s) 258
KspAI GTTAAC 2 cut(s) 637, 643
Kzo9I GATC 6 cut(s) 14, 112, 522, 721, 730, 758
LmnI GCTCC 2 cut(s) 89, 397
LpnPI CCDG 8 cut(s) 300, 359, 395, 395, 422, 511, 517, 645
Lsp1109I GCAGC 4 cut(s) 242, 291, 295, 676
MaeI CTAG 1 cut(s) 12
MaeII ACGT 1 cut(s) 639
MaeIII GTNAC 1 cut(s) 647
MalI GATC 6 cut(s) 16, 114, 524, 723, 732, 760
MboI GATC 6 cut(s) 14, 112, 522, 721, 730, 758
MflI RGATCY 4 cut(s) 14, 112, 522, 721
MhlI GDGCHC 2 cut(s) 359, 565
MluCI AATT 2 cut(s) 448, 748
MlyI GAGTC 1 cut(s) 622
MmeI TCCRAC 4 cut(s) 42, 46, 444, 650
MnlI CCTC 5 cut(s) 579, 622, 670, 703, 732
MseI TTAA 5 cut(s) 75, 165, 510, 636, 642
MspA1I CMGCKG 1 cut(s) 373
MspI CCGG 3 cut(s) 382, 498, 504
MspR9I CCNGG 3 cut(s) 382, 383, 410
MvaI CCWGG 1 cut(s) 410
MvnI CGCG 2 cut(s) 431, 439
MwoI GCNNNNNNNGC 2 cut(s) 261, 442
NciI CCSGG 2 cut(s) 382, 383
NdeII GATC 6 cut(s) 14, 112, 522, 721, 730, 758
NlaIII CATG 1 cut(s) 737
NlaIV GGNNCC 3 cut(s) 368, 378, 502
NruI TCGCGA 1 cut(s) 431
PcsI WCGNNNNNNNCGW 1 cut(s) 356
PfeI GAWTC 2 cut(s) 475, 553
PkrI GCNGC 5 cut(s) 257, 285, 306, 438, 691
PleI GAGTC 1 cut(s) 622
PpsI GAGTC 1 cut(s) 622
Psp124BI GAGCTC 1 cut(s) 359
Psp1406I AACGTT 1 cut(s) 639
Psp6I CCWGG 1 cut(s) 408
PspGI CCWGG 1 cut(s) 408
PspN4I GGNNCC 3 cut(s) 368, 378, 502
PspPI GGNCC 4 cut(s) 8, 366, 376, 406
PsuI RGATCY 4 cut(s) 14, 112, 522, 721
RruI TCGCGA 1 cut(s) 431
SacI GAGCTC 1 cut(s) 359
SaqAI TTAA 5 cut(s) 75, 165, 510, 636, 642
SatI GCNGC 5 cut(s) 256, 284, 305, 437, 690
Sau3AI GATC 6 cut(s) 14, 112, 522, 721, 730, 758
Sau96I GGNCC 4 cut(s) 8, 366, 376, 406
SchI GAGTC 1 cut(s) 622
ScrFI CCNGG 3 cut(s) 382, 383, 410
SduI GDGCHC 2 cut(s) 359, 565
SinI GGWCC 3 cut(s) 366, 376, 406
SmaI CCCGGG 1 cut(s) 383
Sse9I AATT 2 cut(s) 448, 748
SsiI CCGC 3 cut(s) 330, 371, 437
SspMI CTAG 1 cut(s) 12
SstI GAGCTC 1 cut(s) 359
StyD4I CCNGG 3 cut(s) 380, 381, 408
TaaI ACNGT 1 cut(s) 647
TaiI ACGT 1 cut(s) 642
TaqI TCGA 4 cut(s) 342, 359, 492, 662
TasI AATT 2 cut(s) 448, 748
TauI GCSGC 1 cut(s) 439
TfiI GAWTC 2 cut(s) 475, 553
Tru1I TTAA 5 cut(s) 75, 165, 510, 636, 642
Tru9I TTAA 5 cut(s) 75, 165, 510, 636, 642
TscAI CASTG 1 cut(s) 294
TseI GCWGC 4 cut(s) 255, 283, 304, 689
TspMI CCCGGG 1 cut(s) 381
TspRI CASTG 1 cut(s) 294
VpaK11BI GGWCC 3 cut(s) 366, 376, 406
XmaI CCCGGG 1 cut(s) 381
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.