MD17G1129800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014460
Physical Location & Seq
Reverse (-)
11431893 .. 11433143
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1129800.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGCAGTAGAAGATGAAGCATTTTCTCTCACTTCACTCAAACAACAAGAAGAAGAAGAGAGAGAGAGTTTTTTAGCTTTGAGCAGCACTTGCAAAGTGGTGGAGTATCTGCAGCCAGTGATGTCCAAAGAGCTCCTCTGCAAGTTCCCGGACAACTCCGCTTTTGATTTCGACTACACGCAGAGCTCGATATGGTCCCCGTTGGTCCCCCGGGCTTACGCTCCCATGGATTTGGATATGGACTTGGATTTGGATTTCTGGAGACCGCGAAAGCTCAATTTCGAGATGGGGTTGGAGGTGAAGAATCAGAAAAGCAGCCTCGTTGACACTGGAGCCGGTTCGGGCATTAAGAAAAAGAAGATCTCCACAACTGCAACTTGTTTCAATCTGAATCAGAGTGCTCTGAAGAACAAGGTGATGAAGAGGAAGAAAAGCAAGATGGTTTTGGCCTCTGACTTCTTTCCAACTCCTGTGAAGGTTAACTGTAACCCCATTGCATCAAAGGTGTGGAATAAGGTGCTTAAAGCTGCCTCAAAGCATTTCAAGAAAAAGAAGAGAGATCCCATGGATCATGTGAGGCTCTCAAATTATTTGAGATCAGATGGAAATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

23.26

Weight (kDa)

9.33

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014460)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15140
fragaria_vesca FvH4_6g39300
malus_domestica MD09G1143500.v1.1 MD17G1129800.v1.1
prunus_persica Prupe.3G184300_v2.0.a1
pyrus_communis pycom09g06300 pycom17g12240
rosa_chinensis RchiOBHm_Chr2g0153221 RchiOBHm_Chr3g0480451
rosa_multiflora Rmu_sc0000442.1_g000003
rosa_roxburghii Rroxscaffold_2G00095090 Rroxscaffold_5G00335020
rosa_rugosa Rorug02G0437600
rosa_samantha Rh2BG512100 Rh2CG486800 Rh4CG218900
rosa_wichuraiana Rw2G041080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 268
AciI CCGC 2 cut(s) 159, 266
AclWI GGATC 2 cut(s) 554, 576
AcuI CTGAAG 1 cut(s) 425
AgsI TTSAA 2 cut(s) 385, 544
AluBI AGCT 5 cut(s) 77, 133, 186, 274, 527
AluI AGCT 5 cut(s) 77, 133, 186, 274, 527
Alw21I GWGCWC 3 cut(s) 135, 188, 403
Alw26I GTCTC 1 cut(s) 256
AlwI GGATC 2 cut(s) 554, 576
Ama87I CYCGRG 1 cut(s) 210
AoxI GGCC 1 cut(s) 447
ApeKI GCWGC 4 cut(s) 84, 112, 315, 527
AspS9I GGNCC 2 cut(s) 195, 205
AsuC2I CCSGG 3 cut(s) 149, 211, 212
AsuHPI GGTGA 2 cut(s) 310, 427
AvaI CYCGRG 1 cut(s) 210
AvaII GGWCC 2 cut(s) 195, 205
BanII GRGCYC 2 cut(s) 135, 188
Bbv12I GWGCWC 3 cut(s) 135, 188, 403
BbvI GCAGC 4 cut(s) 96, 124, 327, 514
BccI CCATC 3 cut(s) 280, 433, 596
BcnI CCSGG 3 cut(s) 149, 211, 212
BcoDI GTCTC 1 cut(s) 256
BfmI CTRYAG 1 cut(s) 110
BglII AGATCT 1 cut(s) 360
BisI GCNGC 4 cut(s) 85, 113, 316, 528
BlsI GCNGC 4 cut(s) 86, 114, 317, 529
Bme1390I CCNGG 3 cut(s) 149, 211, 212
Bme18I GGWCC 2 cut(s) 195, 205
BmeT110I CYCGRG 1 cut(s) 210
BmgT120I GGNCC 2 cut(s) 195, 205
BmiI GGNNCC 3 cut(s) 197, 207, 334
BmrFI CCNGG 3 cut(s) 149, 211, 212
BmsI GCATC 1 cut(s) 506
BpmI CTGGAG 2 cut(s) 280, 351
BpuMI CCSGG 3 cut(s) 149, 211, 212
BsaI GGTCTC 1 cut(s) 256
BsaJI CCNNGG 4 cut(s) 209, 210, 225, 564
Bse118I RCCGGY 1 cut(s) 335
Bse1I ACTGG 2 cut(s) 116, 334
Bse3DI GCAATG 1 cut(s) 492
BseDI CCNNGG 4 cut(s) 209, 210, 225, 564
BseMI GCAATG 1 cut(s) 492
BseNI ACTGG 2 cut(s) 116, 334
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 4 cut(s) 96, 124, 327, 514
Bsh1236I CGCG 1 cut(s) 268
BshFI GGCC 1 cut(s) 449
BsiHKAI GWGCWC 3 cut(s) 135, 188, 403
BsiHKCI CYCGRG 1 cut(s) 210
BsiSI CCGG 3 cut(s) 149, 211, 336
BslFI GGGAC 2 cut(s) 181, 191
BsmAI GTCTC 1 cut(s) 256
BsmFI GGGAC 2 cut(s) 181, 191
BsnI GGCC 1 cut(s) 449
Bso31I GGTCTC 1 cut(s) 256
BsoBI CYCGRG 1 cut(s) 210
Bsp1286I GDGCHC 3 cut(s) 135, 188, 403
Bsp143I GATC 4 cut(s) 360, 559, 568, 596
Bsp19I CCATGG 2 cut(s) 225, 564
BspACI CCGC 2 cut(s) 159, 266
BspANI GGCC 1 cut(s) 449
BspFNI CGCG 1 cut(s) 268
BspLI GGNNCC 3 cut(s) 197, 207, 334
BspMAI CTGCAG 1 cut(s) 114
BspPI GGATC 2 cut(s) 554, 576
BspTNI GGTCTC 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 492
BsrFI RCCGGY 1 cut(s) 335
BsrI ACTGG 2 cut(s) 116, 334
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 4 cut(s) 209, 210, 225, 564
BssMI GATC 4 cut(s) 360, 559, 568, 596
BssT1I CCWWGG 2 cut(s) 225, 564
Bst4CI ACNGT 1 cut(s) 485
Bst6I CTCTTC 3 cut(s) 51, 416, 548
BstAPI GCANNNNNTGC 1 cut(s) 90
BstDSI CCRYGG 2 cut(s) 225, 564
BstFNI CGCG 1 cut(s) 268
BstKTI GATC 4 cut(s) 363, 562, 571, 599
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 4 cut(s) 360, 559, 568, 596
BstMWI GCNNNNNNNGC 1 cut(s) 90
BstSCI CCNGG 3 cut(s) 147, 209, 210
BstSFI CTRYAG 1 cut(s) 110
BstUI CGCG 1 cut(s) 268
BstV1I GCAGC 4 cut(s) 96, 124, 327, 514
BstX2I RGATCY 2 cut(s) 360, 559
BstXI CCANNNNNNTGG 1 cut(s) 232
BstYI RGATCY 2 cut(s) 360, 559
BsuRI GGCC 1 cut(s) 449
BtgI CCRYGG 2 cut(s) 225, 564
BtsIMutI CAGTG 2 cut(s) 123, 327
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 2 cut(s) 195, 205
Cfr9I CCCGGG 1 cut(s) 210
CviAII CATG 3 cut(s) 226, 565, 572
DpnI GATC 4 cut(s) 362, 561, 570, 598
DpnII GATC 4 cut(s) 360, 559, 568, 596
Eam1104I CTCTTC 3 cut(s) 51, 416, 548
EarI CTCTTC 3 cut(s) 51, 416, 548
Ecl136II GAGCTC 2 cut(s) 133, 186
Eco130I CCWWGG 2 cut(s) 225, 564
Eco24I GRGCYC 2 cut(s) 135, 188
Eco31I GGTCTC 1 cut(s) 256
Eco47I GGWCC 2 cut(s) 195, 205
Eco53kI GAGCTC 2 cut(s) 133, 186
Eco57I CTGAAG 1 cut(s) 425
Eco88I CYCGRG 1 cut(s) 210
EcoICRI GAGCTC 2 cut(s) 133, 186
EcoT14I CCWWGG 2 cut(s) 225, 564
EcoT38I GRGCYC 2 cut(s) 135, 188
ErhI CCWWGG 2 cut(s) 225, 564
FaeI CATG 3 cut(s) 229, 568, 575
FaiI YATR 5 cut(s) 193, 227, 239, 566, 573
FaqI GGGAC 2 cut(s) 181, 191
FatI CATG 3 cut(s) 225, 564, 571
Fnu4HI GCNGC 4 cut(s) 85, 113, 316, 528
FriOI GRGCYC 2 cut(s) 135, 188
Fsp4HI GCNGC 4 cut(s) 85, 113, 316, 528
GluI GCNGC 4 cut(s) 85, 113, 316, 528
GsuI CTGGAG 2 cut(s) 280, 351
HaeIII GGCC 1 cut(s) 449
HapII CCGG 3 cut(s) 149, 211, 336
Hin1II CATG 3 cut(s) 229, 568, 575
HincII GTYRAC 2 cut(s) 325, 481
HindII GTYRAC 2 cut(s) 325, 481
HinfI GANTC 2 cut(s) 304, 391
HpaI GTTAAC 1 cut(s) 481
HpaII CCGG 3 cut(s) 149, 211, 336
HphI GGTGA 2 cut(s) 310, 427
Hpy166II GTNNAC 2 cut(s) 325, 481
Hpy188I TCNGA 6 cut(s) 309, 390, 396, 405, 454, 601
Hpy188III TCNNGA 3 cut(s) 259, 283, 544
Hpy8I GTNNAC 2 cut(s) 325, 481
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4V TGCA 5 cut(s) 93, 112, 141, 374, 497
HpyF10VI GCNNNNNNNGC 1 cut(s) 90
Hsp92II CATG 3 cut(s) 229, 568, 575
KspAI GTTAAC 1 cut(s) 481
Kzo9I GATC 4 cut(s) 360, 559, 568, 596
LmnI GCTCC 3 cut(s) 138, 226, 332
LpnPI CCDG 7 cut(s) 129, 162, 224, 244, 315, 349, 483
Lsp1109I GCAGC 4 cut(s) 96, 124, 327, 514
LweI GCATC 1 cut(s) 506
MaeIII GTNAC 1 cut(s) 485
MalI GATC 4 cut(s) 362, 561, 570, 598
MboI GATC 4 cut(s) 360, 559, 568, 596
MflI RGATCY 2 cut(s) 360, 559
MhlI GDGCHC 3 cut(s) 135, 188, 403
MluCI AATT 2 cut(s) 277, 586
MmeI TCCRAC 2 cut(s) 273, 488
MnlI CCTC 7 cut(s) 146, 289, 329, 417, 460, 541, 570
MseI TTAA 3 cut(s) 348, 480, 522
MspI CCGG 3 cut(s) 149, 211, 336
MspR9I CCNGG 3 cut(s) 149, 211, 212
MvnI CGCG 1 cut(s) 268
MwoI GCNNNNNNNGC 1 cut(s) 90
NciI CCSGG 3 cut(s) 149, 211, 212
NcoI CCATGG 2 cut(s) 225, 564
NdeII GATC 4 cut(s) 360, 559, 568, 596
NlaIII CATG 3 cut(s) 229, 568, 575
NlaIV GGNNCC 3 cut(s) 197, 207, 334
PcsI WCGNNNNNNNCGW 1 cut(s) 185
PfeI GAWTC 2 cut(s) 304, 391
PfoI TCCNGGA 1 cut(s) 147
PkrI GCNGC 4 cut(s) 86, 114, 317, 529
Psp124BI GAGCTC 2 cut(s) 135, 188
PspN4I GGNNCC 3 cut(s) 197, 207, 334
PspPI GGNCC 2 cut(s) 195, 205
PstI CTGCAG 1 cut(s) 114
PsuI RGATCY 2 cut(s) 360, 559
SacI GAGCTC 2 cut(s) 135, 188
SaqAI TTAA 3 cut(s) 348, 480, 522
SatI GCNGC 4 cut(s) 85, 113, 316, 528
Sau3AI GATC 4 cut(s) 360, 559, 568, 596
Sau96I GGNCC 2 cut(s) 195, 205
ScrFI CCNGG 3 cut(s) 149, 211, 212
SduI GDGCHC 3 cut(s) 135, 188, 403
SfaNI GCATC 1 cut(s) 506
SfcI CTRYAG 1 cut(s) 110
SinI GGWCC 2 cut(s) 195, 205
SmaI CCCGGG 1 cut(s) 212
Sse9I AATT 2 cut(s) 277, 586
SsiI CCGC 2 cut(s) 159, 266
SspI AATATT 1 cut(s) 610
SstI GAGCTC 2 cut(s) 135, 188
StyD4I CCNGG 3 cut(s) 147, 209, 210
StyI CCWWGG 2 cut(s) 225, 564
TaaI ACNGT 1 cut(s) 485
TaqI TCGA 3 cut(s) 171, 188, 282
TasI AATT 2 cut(s) 277, 586
TfiI GAWTC 2 cut(s) 304, 391
Tru1I TTAA 3 cut(s) 348, 480, 522
Tru9I TTAA 3 cut(s) 348, 480, 522
TscAI CASTG 2 cut(s) 123, 334
TseI GCWGC 4 cut(s) 84, 112, 315, 527
TspDTI ATGAA 2 cut(s) 30, 434
TspMI CCCGGG 1 cut(s) 210
TspRI CASTG 2 cut(s) 123, 334
VpaK11BI GGWCC 2 cut(s) 195, 205
XmaI CCCGGG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.