Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0007463

Physical Location & Seq

Genomic Coordinates Forward (+)

11742780 .. 11746728

Gene Structure 3949 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1149500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1383 bp

ATGTGGTGGTGGGTAGATATTAGACGCATTTGCTCGCTTTCTCTCTACCAAACCAAAACGTATGCTCTCTCTCTACCAAACCAAATCATATGCTCTCTCTCTCTCTCTCTCTCTCTCTCTACCAGACCAGTTACTTGGGATGCTATCAACAACAACACATTAATAACCCCATCACCTTCTCTTCACAACCCAACTTTCCTCTTTGCAACTTCTTCTTCTCCTGATAACGAAGTTGTAACTTCTGATGAGGTTCTTGTTGGAGAAGAAATAGAGCGGGTGAGGTTGATCACACTGAACAGGCCTCGTCAATTGAATGTCATCTCATCCAAAGTGGTTTCTTTGCTAGCTGAGTACTTGGAAAAGTGGGAAAAAGATGACAATGCTGAGCTTGTAATTATCAAGGGATCAGGCCGAGCTTTTTCAGCTGGCGGGGACTTGAAAATGTTCTATGACGGCAGGAAATCAAAGGATTCCTGCCTTGAAGTTGTTTACAGAATGTATTGGCTTTGCTACCACATTCATACTTACAAGAAAACACAGGTGGCTCTGGTCCACGGAATTTCAATGGGTGGAGGCGCATCTTTGATGGTCCCAATGAAGTTCTCAGTAGTCACTGAAAAAACTGTTTTTTCCACTCCAGAAGCTAGTATTGGATTTCATACAGATTGCGGTTTCTCATATATGCATTCCCATCTTCCTGGGTTTCTCGGGGAGTTTTTGGGCTTAACAGGAGCAAGACTGAATGGTAAGGAACTGGTTGCGGTTGGACTGGCAACCCATTTTGTCCCTCTTGAGAAATTATCCGATCTAGAGAAGCGTTTGATAAGTTTGAACTCCGGAAATGAGAACGCGGTCAAAGCTGCAATTGAAGAATTTTCTGTGGAAGTCAAGCCTGATGAAGAAAGTGTTTTAAACAAGCAGTCAATAATTGACGAGTGCTTCTCCAAAGATACAGTGGCTGAGATTATACAATCATTTGAAGTAGAGGCAAAGAAAGAAGGAAATGGATGGATAGGTCCACATCTCAAGGGCTTAAAAAGATCATCCCCTACGGGATTGAGAATAACACTGAGATCGATTCGAGAAGGCAGGAAGCAAACGTTGCCTGAATCTCTGAAGAAGGAATTTAGACTCACAATAAATATACTGCGGTCCAAAATTTCTGAGGATGTTTATGAGGGTATTAGAGCTCTCACTATTGACAAGGATAATGCTCCCAAGTGGAACCCGCCGTCTCTTGACAATGTGGTGGATAAGAAACTAGACCTGGTCTTCCAGCCGTTCGAAGAAGATTTGGAGCTCCAGATTAAGGAACAGGAAGAAAACAGGTGGGATGGCAAGTATGAGAATTCAGCTTATGCATCCCTGAAGGTGACCGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0008150 GO:0009628 GO:0050896 GO:0080167

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

461

Amino Acids

51.7

Weight (kDa)

5.95

Isoelectric Point (pI)

44.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	91 - 326	2.6e-29	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	93 - 425	8.2e-112	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007463)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G06550
fragaria_vesca	FvH4_6g38591 FvH4_6g38591 FvH4_6g38591
malus_domestica	MD09G1149500.v1.1
prunus_persica	Prupe.3G176700_v2.0.a1 Prupe.3G176700_v2.0.a1 Prupe.3G176700_v2.0.a1 Prupe.3G176700_v2.0.a1 Prupe.3G176700_v2.0.a1 Prupe.3G176900_v2.0.a1 Prupe.3G176900_v2.0.a1 Prupe.3G176900_v2.0.a1 Prupe.3G176900_v2.0.a1
pyrus_communis	pycom09g06880
rosa_chinensis	RchiOBHm_Chr2g0152011
rosa_laevigata	RLG00000020600
rosa_multiflora	Rmu_sc0017048.1_g000001
rosa_roxburghii	Rroxscaffold_2G00096050
rosa_rugosa	Rorug02G0430400
rosa_samantha	Rh2AG491000 Rh2BG503300 Rh2CG477400 Rh2DG514600
rosa_wichuraiana	Rw2G040360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	274
AccII	CGCG	1 cut(s)	851
AccIII	TCCGGA	1 cut(s)	836
AciI	CCGC	7 cut(s)	274, 429, 669, 761, 851, 1150, 1229
AclI	AACGTT	1 cut(s)	1100
AclWI	GGATC	1 cut(s)	412
AcsI	RAATTY	5 cut(s)	558, 872, 1124, 1158, 1348
AcuI	CTGAAG	1 cut(s)	1136
AfaI	GTAC	1 cut(s)	353
AfiI	CCNNNNNNNGG	1 cut(s)	1309
AgsI	TTSAA	7 cut(s)	313, 439, 482, 564, 832, 869, 980
AjnI	CCWGG	2 cut(s)	697, 1266
AjuI	GAANNNNNNNTTGG	2 cut(s)	633, 665
AluBI	AGCT	9 cut(s)	347, 388, 416, 425, 644, 860, 1190, 1300, 1355
AluI	AGCT	9 cut(s)	347, 388, 416, 425, 644, 860, 1190, 1300, 1355
Alw21I	GWGCWC	2 cut(s)	1192, 1302
Alw26I	GTCTC	1 cut(s)	1239
AlwI	GGATC	1 cut(s)	412
AlwNI	CAGNNNCTG	1 cut(s)	959
Ama87I	CYCGRG	1 cut(s)	707
Aor13HI	TCCGGA	1 cut(s)	836
AoxI	GGCC	2 cut(s)	299, 409
ApeKI	GCWGC	1 cut(s)	860
ApoI	RAATTY	5 cut(s)	558, 872, 1124, 1158, 1348
AseI	ATTAAT	1 cut(s)	161
Asp700I	GAANNNNTTC	1 cut(s)	443
AspLEI	GCGC	1 cut(s)	578
AspS9I	GGNCC	4 cut(s)	550, 589, 1016, 1152
AsuC2I	CCSGG	1 cut(s)	1378
AsuHPI	GGTGA	2 cut(s)	165, 289
AsuII	TTCGAA	1 cut(s)	1284
AsuNHI	GCTAGC	1 cut(s)	343
AvaI	CYCGRG	1 cut(s)	707
AvaII	GGWCC	4 cut(s)	550, 589, 1016, 1152
BanII	GRGCYC	2 cut(s)	1192, 1302
BbsI	GAAGAC	1 cut(s)	1264
Bbv12I	GWGCWC	2 cut(s)	1192, 1302
BbvI	GCAGC	1 cut(s)	847
BccI	CCATC	5 cut(s)	178, 580, 699, 1002, 1328
BceAI	ACGGC	3 cut(s)	469, 1216, 1264
BciT130I	CCWGG	2 cut(s)	699, 1268
BclI	TGATCA	1 cut(s)	285
BcnI	CCSGG	1 cut(s)	1378
BcoDI	GTCTC	1 cut(s)	1239
BfaI	CTAG	4 cut(s)	344, 645, 809, 1262
BisI	GCNGC	1 cut(s)	861
BlpI	GCTNAGC	1 cut(s)	384
BlsI	GCNGC	1 cut(s)	862
BmcAI	AGTACT	1 cut(s)	353
Bme1390I	CCNGG	3 cut(s)	699, 1268, 1378
Bme18I	GGWCC	4 cut(s)	550, 589, 1016, 1152
BmeT110I	CYCGRG	1 cut(s)	707
BmgT120I	GGNCC	4 cut(s)	550, 589, 1016, 1152
BmiI	GGNNCC	2 cut(s)	591, 1226
BmrFI	CCNGG	3 cut(s)	699, 1268, 1378
BmsI	GCATC	3 cut(s)	130, 587, 1370
BmtI	GCTAGC	1 cut(s)	347
BpiI	GAAGAC	1 cut(s)	1264
BplI	GAGNNNNNCTC	2 cut(s)	926, 958
BpmI	CTGGAG	2 cut(s)	621, 1286
Bpu1102I	GCTNAGC	1 cut(s)	384
Bpu14I	TTCGAA	1 cut(s)	1284
BpuEI	CTTGAG	2 cut(s)	812, 1010
BpuMI	CCSGG	1 cut(s)	1378
Bsa29I	ATCGAT	1 cut(s)	1076
BsaJI	CCNNGG	2 cut(s)	553, 698
BsaWI	WCCGGW	1 cut(s)	836
Bsc4I	CCNNNNNNNGG	1 cut(s)	1309
Bse1I	ACTGG	3 cut(s)	128, 759, 774
BseAI	TCCGGA	1 cut(s)	836
BseBI	CCWGG	2 cut(s)	699, 1268
BseCI	ATCGAT	1 cut(s)	1076
BseDI	CCNNGG	2 cut(s)	553, 698
BseGI	GGATG	7 cut(s)	145, 323, 1013, 1043, 1174, 1339, 1361
BseLI	CCNNNNNNNGG	1 cut(s)	1309
BseMII	CTCAG	6 cut(s)	339, 375, 618, 951, 1061, 1155
BseNI	ACTGG	3 cut(s)	128, 759, 774
BseXI	GCAGC	1 cut(s)	847
Bsh1236I	CGCG	1 cut(s)	851
BshFI	GGCC	2 cut(s)	301, 411
BshVI	ATCGAT	1 cut(s)	1076
BsiHKAI	GWGCWC	2 cut(s)	1192, 1302
BsiHKCI	CYCGRG	1 cut(s)	707
BsiSI	CCGG	2 cut(s)	837, 1377
BslFI	GGGAC	3 cut(s)	446, 575, 770
BslI	CCNNNNNNNGG	1 cut(s)	1309
BsmAI	GTCTC	1 cut(s)	1239
BsmBI	CGTCTC	1 cut(s)	1239
BsmFI	GGGAC	3 cut(s)	446, 575, 770
BsmI	GAATGC	1 cut(s)	685
BsnI	GGCC	2 cut(s)	301, 411
BsoBI	CYCGRG	1 cut(s)	707
Bsp119I	TTCGAA	1 cut(s)	1284
Bsp1286I	GDGCHC	2 cut(s)	1192, 1302
Bsp13I	TCCGGA	1 cut(s)	836
Bsp143I	GATC	5 cut(s)	285, 404, 805, 1040, 1073
Bsp1720I	GCTNAGC	1 cut(s)	384
BspACI	CCGC	7 cut(s)	274, 429, 669, 761, 851, 1150, 1229
BspANI	GGCC	2 cut(s)	301, 411
BspCNI	CTCAG	6 cut(s)	340, 376, 617, 952, 1062, 1156
BspDI	ATCGAT	1 cut(s)	1076
BspEI	TCCGGA	1 cut(s)	836
BspFNI	CGCG	1 cut(s)	851
BspLI	GGNNCC	2 cut(s)	591, 1226
BspOI	GCTAGC	1 cut(s)	347
BspPI	GGATC	1 cut(s)	412
BspT104I	TTCGAA	1 cut(s)	1284
BsrBI	CCGCTC	1 cut(s)	274
BsrI	ACTGG	3 cut(s)	128, 759, 774
BssECI	CCNNGG	2 cut(s)	553, 698
BssMI	GATC	5 cut(s)	285, 404, 805, 1040, 1073
Bst2UI	CCWGG	2 cut(s)	699, 1268
Bst4CI	ACNGT	2 cut(s)	625, 955
Bst6I	CTCTTC	1 cut(s)	186
BstAPI	GCANNNNNTGC	1 cut(s)	1102
BstBI	TTCGAA	1 cut(s)	1284
BstC8I	GCNNGC	3 cut(s)	35, 345, 427
BstDEI	CTNAG	6 cut(s)	348, 384, 604, 960, 1070, 1164
BstDSI	CCRYGG	1 cut(s)	553
BstEII	GGTNACC	1 cut(s)	1372
BstF5I	GGATG	7 cut(s)	145, 323, 1013, 1043, 1174, 1339, 1361
BstFNI	CGCG	1 cut(s)	851
BstHHI	GCGC	1 cut(s)	578
BstKTI	GATC	5 cut(s)	288, 407, 808, 1043, 1076
BstMAI	GTCTC	1 cut(s)	1239
BstMBI	GATC	5 cut(s)	285, 404, 805, 1040, 1073
BstMWI	GCNNNNNNNGC	3 cut(s)	422, 857, 1102
BstNI	CCWGG	2 cut(s)	699, 1268
BstPI	GGTNACC	1 cut(s)	1372
BstSCI	CCNGG	3 cut(s)	697, 1266, 1376
BstUI	CGCG	1 cut(s)	851
BstV1I	GCAGC	1 cut(s)	847
BstV2I	GAAGAC	1 cut(s)	1264
BstXI	CCANNNNNNTGG	2 cut(s)	135, 698
Bsu15I	ATCGAT	1 cut(s)	1076
BsuRI	GGCC	2 cut(s)	301, 411
BsuTUI	ATCGAT	1 cut(s)	1076
BtgI	CCRYGG	1 cut(s)	553
BtsCI	GGATG	7 cut(s)	145, 323, 1013, 1043, 1174, 1339, 1361
BtsIMutI	CAGTG	4 cut(s)	290, 612, 960, 1067
Cac8I	GCNNGC	3 cut(s)	35, 345, 427
CaiI	CAGNNNCTG	1 cut(s)	959
CfoI	GCGC	1 cut(s)	578
Cfr13I	GGNCC	4 cut(s)	550, 589, 1016, 1152
ClaI	ATCGAT	1 cut(s)	1076
CseI	GACGC	1 cut(s)	33
CsiI	ACCWGGT	1 cut(s)	1266
Csp6I	GTAC	1 cut(s)	352
CviQI	GTAC	1 cut(s)	352
DdeI	CTNAG	6 cut(s)	348, 384, 604, 960, 1070, 1164
DpnI	GATC	5 cut(s)	287, 406, 807, 1042, 1075
DpnII	GATC	5 cut(s)	285, 404, 805, 1040, 1073
DraI	TTTAAA	1 cut(s)	912
Eam1104I	CTCTTC	1 cut(s)	186
EarI	CTCTTC	1 cut(s)	186
Ecl136II	GAGCTC	2 cut(s)	1190, 1300
Eco147I	AGGCCT	1 cut(s)	301
Eco24I	GRGCYC	2 cut(s)	1192, 1302
Eco47I	GGWCC	4 cut(s)	550, 589, 1016, 1152
Eco53kI	GAGCTC	2 cut(s)	1190, 1300
Eco57I	CTGAAG	1 cut(s)	1136
Eco88I	CYCGRG	1 cut(s)	707
Eco91I	GGTNACC	1 cut(s)	1372
EcoICRI	GAGCTC	2 cut(s)	1190, 1300
EcoO65I	GGTNACC	1 cut(s)	1372
EcoRI	GAATTC	1 cut(s)	1348
EcoRII	CCWGG	2 cut(s)	697, 1266
EcoT22I	ATGCAT	2 cut(s)	687, 1363
EcoT38I	GRGCYC	2 cut(s)	1192, 1302
Esp3I	CGTCTC	1 cut(s)	1239
FaqI	GGGAC	3 cut(s)	446, 575, 770
FauI	CCCGC	3 cut(s)	267, 422, 1236
FauNDI	CATATG	1 cut(s)	89
FbaI	TGATCA	1 cut(s)	285
Fnu4HI	GCNGC	1 cut(s)	861
FokI	GGATG	7 cut(s)	152, 310, 1020, 1030, 1181, 1346, 1348
FriOI	GRGCYC	2 cut(s)	1192, 1302
Fsp4HI	GCNGC	1 cut(s)	861
FspBI	CTAG	4 cut(s)	344, 645, 809, 1262
GlaI	GCGC	1 cut(s)	577
GluI	GCNGC	1 cut(s)	861
GsuI	CTGGAG	2 cut(s)	621, 1286
HaeIII	GGCC	2 cut(s)	301, 411
HapII	CCGG	2 cut(s)	837, 1377
HgaI	GACGC	1 cut(s)	33
HhaI	GCGC	1 cut(s)	578
Hin6I	GCGC	1 cut(s)	576
HinP1I	GCGC	1 cut(s)	576
HinfI	GANTC	4 cut(s)	470, 1078, 1109, 1131
HpaII	CCGG	2 cut(s)	837, 1377
HphI	GGTGA	2 cut(s)	165, 289
Hpy166II	GTNNAC	3 cut(s)	490, 553, 1019
Hpy188I	TCNGA	4 cut(s)	244, 805, 1116, 1165
Hpy188III	TCNNGA	8 cut(s)	221, 638, 791, 809, 837, 1082, 1238, 1303
Hpy8I	GTNNAC	3 cut(s)	490, 553, 1019
HpyAV	CCTTC	5 cut(s)	186, 992, 1079, 1114, 1363
HpyCH4III	ACNGT	2 cut(s)	625, 955
HpyCH4IV	ACGT	2 cut(s)	59, 1100
HpyCH4V	TGCA	4 cut(s)	206, 685, 863, 1361
HpyF10VI	GCNNNNNNNGC	3 cut(s)	422, 857, 1102
HpyF3I	CTNAG	6 cut(s)	348, 384, 604, 960, 1070, 1164
HpySE526I	ACGT	2 cut(s)	59, 1100
HspAI	GCGC	1 cut(s)	576
Kpn2I	TCCGGA	1 cut(s)	836
Ksp22I	TGATCA	1 cut(s)	285
Kzo9I	GATC	5 cut(s)	285, 404, 805, 1040, 1073
LmnI	GCTCC	4 cut(s)	731, 1219, 1297, 1305
Lsp1109I	GCAGC	1 cut(s)	847
LweI	GCATC	3 cut(s)	130, 587, 1370
MabI	ACCWGGT	1 cut(s)	1266
MaeI	CTAG	4 cut(s)	344, 645, 809, 1262
MaeII	ACGT	2 cut(s)	59, 1100
MaeIII	GTNAC	4 cut(s)	130, 235, 610, 1372
MalI	GATC	5 cut(s)	287, 406, 807, 1042, 1075
MbiI	CCGCTC	1 cut(s)	274
MboI	GATC	5 cut(s)	285, 404, 805, 1040, 1073
MfeI	CAATTG	2 cut(s)	308, 864
MhlI	GDGCHC	2 cut(s)	1192, 1302
MlyI	GAGTC	1 cut(s)	1125
MmeI	TCCRAC	2 cut(s)	238, 745
MnlI	CCTC	9 cut(s)	209, 241, 273, 312, 566, 798, 979, 1159, 1171
Mph1103I	ATGCAT	2 cut(s)	687, 1363
MroI	TCCGGA	1 cut(s)	836
MroXI	GAANNNNTTC	1 cut(s)	443
MseI	TTAA	5 cut(s)	161, 725, 911, 1034, 1308
MspA1I	CMGCKG	1 cut(s)	425
MspI	CCGG	2 cut(s)	837, 1377
MspR9I	CCNGG	3 cut(s)	699, 1268, 1378
MunI	CAATTG	2 cut(s)	308, 864
Mva1269I	GAATGC	1 cut(s)	685
MvaI	CCWGG	2 cut(s)	699, 1268
MvnI	CGCG	1 cut(s)	851
MwoI	GCNNNNNNNGC	3 cut(s)	422, 857, 1102
NciI	CCSGG	1 cut(s)	1378
NdeI	CATATG	1 cut(s)	89
NdeII	GATC	5 cut(s)	285, 404, 805, 1040, 1073
NheI	GCTAGC	1 cut(s)	343
NlaIV	GGNNCC	2 cut(s)	591, 1226
NmeAIII	GCCGAG	1 cut(s)	437
NmuCI	GTSAC	2 cut(s)	610, 1372
NsiI	ATGCAT	2 cut(s)	687, 1363
NspV	TTCGAA	1 cut(s)	1284
PceI	AGGCCT	1 cut(s)	301
PctI	GAATGC	1 cut(s)	685
PdmI	GAANNNNTTC	1 cut(s)	443
PfeI	GAWTC	3 cut(s)	470, 1078, 1109
PflFI	GACNNNGTC	1 cut(s)	1268
PkrI	GCNGC	1 cut(s)	862
PleI	GAGTC	1 cut(s)	1125
PpsI	GAGTC	1 cut(s)	1125
PshBI	ATTAAT	1 cut(s)	161
Psp124BI	GAGCTC	2 cut(s)	1192, 1302
Psp1406I	AACGTT	1 cut(s)	1100
Psp6I	CCWGG	2 cut(s)	697, 1266
PspEI	GGTNACC	1 cut(s)	1372
PspGI	CCWGG	2 cut(s)	697, 1266
PspN4I	GGNNCC	2 cut(s)	591, 1226
PspPI	GGNCC	4 cut(s)	550, 589, 1016, 1152
PstNI	CAGNNNCTG	1 cut(s)	959
PsyI	GACNNNGTC	1 cut(s)	1268
PvuII	CAGCTG	1 cut(s)	425
RsaI	GTAC	1 cut(s)	353
RsaNI	GTAC	1 cut(s)	352
SacI	GAGCTC	2 cut(s)	1192, 1302
SaqAI	TTAA	5 cut(s)	161, 725, 911, 1034, 1308
SatI	GCNGC	1 cut(s)	861
Sau3AI	GATC	5 cut(s)	285, 404, 805, 1040, 1073
Sau96I	GGNCC	4 cut(s)	550, 589, 1016, 1152
ScaI	AGTACT	1 cut(s)	353
SchI	GAGTC	1 cut(s)	1125
ScrFI	CCNGG	3 cut(s)	699, 1268, 1378
SduI	GDGCHC	2 cut(s)	1192, 1302
SexAI	ACCWGGT	1 cut(s)	1266
SfaNI	GCATC	3 cut(s)	130, 587, 1370
SfuI	TTCGAA	1 cut(s)	1284
SinI	GGWCC	4 cut(s)	550, 589, 1016, 1152
SmlI	CTYRAG	2 cut(s)	791, 1025
SmoI	CTYRAG	2 cut(s)	791, 1025
SseBI	AGGCCT	1 cut(s)	301
SsiI	CCGC	7 cut(s)	274, 429, 669, 761, 851, 1150, 1229
SspMI	CTAG	4 cut(s)	344, 645, 809, 1262
SstI	GAGCTC	2 cut(s)	1192, 1302
StuI	AGGCCT	1 cut(s)	301
StyD4I	CCNGG	3 cut(s)	697, 1266, 1376
TaaI	ACNGT	2 cut(s)	625, 955
TaiI	ACGT	2 cut(s)	62, 1103
TaqI	TCGA	3 cut(s)	1076, 1081, 1284
TatI	WGTACW	1 cut(s)	351
TfiI	GAWTC	3 cut(s)	470, 1078, 1109
Tru1I	TTAA	5 cut(s)	161, 725, 911, 1034, 1308
Tru9I	TTAA	5 cut(s)	161, 725, 911, 1034, 1308
TscAI	CASTG	4 cut(s)	297, 619, 960, 1074
TseFI	GTSAC	2 cut(s)	610, 1372
TseI	GCWGC	1 cut(s)	860
Tsp45I	GTSAC	2 cut(s)	610, 1372
TspDTI	ATGAA	4 cut(s)	509, 611, 647, 912
TspGWI	ACGGA	1 cut(s)	570
TspRI	CASTG	4 cut(s)	297, 619, 960, 1074
Tth111I	GACNNNGTC	1 cut(s)	1268
VpaK11BI	GGWCC	4 cut(s)	550, 589, 1016, 1152
VspI	ATTAAT	1 cut(s)	161
XapI	RAATTY	5 cut(s)	558, 872, 1124, 1158, 1348
XbaI	TCTAGA	1 cut(s)	808
XcmI	CCANNNNNNNNNTGG	1 cut(s)	952
XmnI	GAANNNNTTC	1 cut(s)	443
XspI	CTAG	4 cut(s)	344, 645, 809, 1262
ZrmI	AGTACT	1 cut(s)	353
Zsp2I	ATGCAT	2 cut(s)	687, 1363

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.