MD09G1149600.v1.1

Eukaryotic cytochrome b561

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008301
Physical Location & Seq
Forward (+)
11749329 .. 11752548
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1149600.v1.1.491

Sequence Viewer

Length: 756 bp
ATGAAGTTTCTGCAGAAACTGGGTTTCTTCTGCATTCGTTCAAGTCTTCTTCTTTTTCTTGTTTCGCCGCTCGTCAGCTCCTCTCAAGAACACATGAAGGGTGGAGGCAGTCACAAAGACAACATTCATAAGATGAGTCACAAATTATCGTTTGAAATCAGACTCCACGGGTTTCTTCTTTGGGCTTCGATGGGGTTCTTGATGCCTGTAGGAATACTTGCTATCAGAATGTCACAGAGAGAGGAATGTGGAAGAAGGCTCAGAATTCTTTTCTATGTTCATGGTTTTTCAGAGATGCTCTCTATCCTTCTTGCAACAGCAGCAGCAGTGATGTCCTTCAGAAACTTCAACAACTCCTTCAACAATAAACACCAAAGAGTTGGCTTAGGCCTATATGGCCTCATATGGTTGCAAGCCCTAATAGGGTTTATAAGGCCACAAAGGGGATCCAAGGGAAGAAGCGTATGGTTTTCTGTGCATTGGATTCTTGGAACTGCAGTGTCACTGCTTGGGATCCTCAACATATACACAGGACTACAAGCCTACCATGAGAGGACCTCAAAAGGCATAAAGCTTTGGACCATAATTTTCACTGCTCAAGTCTCTTTCATGACCTTCTTCTACCTATTCCAAGACAAATGGTGGTACATCCAAAAGCAAGGAGTGCTTCTGGGCCATGAACCAGTAAGGCCCACTGCAGATCAAGTCCTTTTGCCAATAGAAAAGCAAAAGCGAGCAGTCACTGATTCTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

252

Amino Acids

28.78

Weight (kDa)

10.26

Isoelectric Point (pI)

61.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 55 - 180 6.7e-09 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 431
AccBSI CCGCTC 1 cut(s) 70
AciI CCGC 1 cut(s) 68
AclWI GGATC 4 cut(s) 441, 454, 508, 521
AcsI RAATTY 1 cut(s) 264
AcuI CTGAAG 1 cut(s) 322
AfaI GTAC 1 cut(s) 647
AfiI CCNNNNNNNGG 2 cut(s) 423, 443
AgsI TTSAA 4 cut(s) 42, 155, 349, 361
AluBI AGCT 2 cut(s) 78, 574
AluI AGCT 2 cut(s) 78, 574
Alw26I GTCTC 1 cut(s) 607
AlwI GGATC 4 cut(s) 441, 454, 508, 521
AlwNI CAGNNNCTG 2 cut(s) 19, 743
AoxI GGCC 5 cut(s) 388, 397, 434, 673, 689
ApeKI GCWGC 2 cut(s) 320, 323
ApoI RAATTY 1 cut(s) 264
AspS9I GGNCC 4 cut(s) 555, 579, 673, 690
AvaII GGWCC 2 cut(s) 555, 579
BamHI GGATCC 2 cut(s) 446, 513
BbsI GAAGAC 1 cut(s) 38
BbvI GCAGC 2 cut(s) 332, 335
BccI CCATC 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 607
BfmI CTRYAG 4 cut(s) 11, 207, 495, 696
BglI GCCNNNNNGGC 1 cut(s) 396
BisI GCNGC 3 cut(s) 68, 321, 324
BlsI GCNGC 3 cut(s) 69, 322, 325
Bme18I GGWCC 2 cut(s) 555, 579
BmgT120I GGNCC 4 cut(s) 555, 579, 673, 690
BmiI GGNNCC 2 cut(s) 448, 515
BmrI ACTGGG 1 cut(s) 29
BmsI GCATC 2 cut(s) 192, 285
BmuI ACTGGG 1 cut(s) 29
BpiI GAAGAC 1 cut(s) 38
BplI GAGNNNNNCTC 4 cut(s) 284, 316, 542, 574
Bpu10I CCTNAGC 1 cut(s) 385
BpuEI CTTGAG 2 cut(s) 69, 582
BsaJI CCNNGG 2 cut(s) 166, 450
Bsc4I CCNNNNNNNGG 2 cut(s) 423, 443
Bse1I ACTGG 2 cut(s) 24, 683
BseDI CCNNGG 2 cut(s) 166, 450
BseGI GGATG 1 cut(s) 648
BseLI CCNNNNNNNGG 2 cut(s) 423, 443
BseMII CTCAG 1 cut(s) 274
BseNI ACTGG 2 cut(s) 24, 683
BseRI GAGGAG 1 cut(s) 70
BseXI GCAGC 2 cut(s) 332, 335
BshFI GGCC 5 cut(s) 390, 399, 436, 675, 691
BslI CCNNNNNNNGG 2 cut(s) 423, 443
BsmAI GTCTC 1 cut(s) 607
BsmI GAATGC 1 cut(s) 33
BsnI GGCC 5 cut(s) 390, 399, 436, 675, 691
Bsp143I GATC 3 cut(s) 446, 513, 700
BspACI CCGC 1 cut(s) 68
BspANI GGCC 5 cut(s) 390, 399, 436, 675, 691
BspCNI CTCAG 1 cut(s) 273
BspHI TCATGA 1 cut(s) 609
BspLI GGNNCC 2 cut(s) 448, 515
BspMAI CTGCAG 3 cut(s) 15, 499, 700
BspPI GGATC 4 cut(s) 441, 454, 508, 521
BsrBI CCGCTC 1 cut(s) 70
BsrI ACTGG 2 cut(s) 24, 683
BssECI CCNNGG 2 cut(s) 166, 450
BssMI GATC 3 cut(s) 446, 513, 700
BssT1I CCWWGG 1 cut(s) 450
BstAPI GCANNNNNTGC 1 cut(s) 664
BstC8I GCNNGC 2 cut(s) 414, 735
BstDEI CTNAG 2 cut(s) 260, 385
BstDSI CCRYGG 1 cut(s) 166
BstF5I GGATG 1 cut(s) 648
BstKTI GATC 3 cut(s) 449, 516, 703
BstMAI GTCTC 1 cut(s) 607
BstMBI GATC 3 cut(s) 446, 513, 700
BstMWI GCNNNNNNNGC 3 cut(s) 320, 396, 664
BstSFI CTRYAG 4 cut(s) 11, 207, 495, 696
BstV1I GCAGC 2 cut(s) 332, 335
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 2 cut(s) 446, 513
BstXI CCANNNNNNTGG 1 cut(s) 380
BstYI RGATCY 2 cut(s) 446, 513
BsuRI GGCC 5 cut(s) 390, 399, 436, 675, 691
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 1 cut(s) 648
BtsI GCAGTG 5 cut(s) 333, 503, 504, 591, 693
BtsIMutI CAGTG 6 cut(s) 333, 503, 504, 591, 693, 741
Cac8I GCNNGC 2 cut(s) 414, 735
CaiI CAGNNNCTG 2 cut(s) 19, 743
CciI TCATGA 1 cut(s) 609
Cfr13I GGNCC 4 cut(s) 555, 579, 673, 690
Csp6I GTAC 1 cut(s) 646
CviAII CATG 5 cut(s) 94, 281, 548, 610, 677
CviQI GTAC 1 cut(s) 646
DdeI CTNAG 2 cut(s) 260, 385
DpnI GATC 3 cut(s) 448, 515, 702
DpnII GATC 3 cut(s) 446, 513, 700
Eco130I CCWWGG 1 cut(s) 450
Eco147I AGGCCT 1 cut(s) 390
Eco47I GGWCC 2 cut(s) 555, 579
Eco57I CTGAAG 1 cut(s) 322
EcoO109I RGGNCCY 1 cut(s) 555
EcoRI GAATTC 1 cut(s) 264
EcoT14I CCWWGG 1 cut(s) 450
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 5 cut(s) 97, 284, 551, 613, 680
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FatI CATG 5 cut(s) 93, 280, 547, 609, 676
FauNDI CATATG 1 cut(s) 404
Fnu4HI GCNGC 3 cut(s) 68, 321, 324
FokI GGATG 1 cut(s) 635
Fsp4HI GCNGC 3 cut(s) 68, 321, 324
GluI GCNGC 3 cut(s) 68, 321, 324
HaeIII GGCC 5 cut(s) 390, 399, 436, 675, 691
Hin1II CATG 5 cut(s) 97, 284, 551, 613, 680
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 4 cut(s) 136, 162, 484, 746
Hpy188I TCNGA 5 cut(s) 161, 227, 263, 292, 341
Hpy188III TCNNGA 3 cut(s) 86, 199, 610
HpyAV CCTTC 6 cut(s) 91, 249, 317, 346, 367, 625
HpyCH4V TGCA 7 cut(s) 13, 33, 314, 412, 478, 497, 698
HpyF10VI GCNNNNNNNGC 3 cut(s) 320, 396, 664
HpyF3I CTNAG 2 cut(s) 260, 385
Hsp92II CATG 5 cut(s) 97, 284, 551, 613, 680
Kzo9I GATC 3 cut(s) 446, 513, 700
LmnI GCTCC 1 cut(s) 83
LpnPI CCDG 5 cut(s) 5, 219, 516, 656, 696
Lsp1109I GCAGC 2 cut(s) 332, 335
LweI GCATC 2 cut(s) 192, 285
MaeIII GTNAC 5 cut(s) 110, 137, 231, 501, 739
MalI GATC 3 cut(s) 448, 515, 702
MbiI CCGCTC 1 cut(s) 70
MboI GATC 3 cut(s) 446, 513, 700
MboII GAAGA 7 cut(s) 19, 38, 41, 167, 264, 468, 610
MflI RGATCY 2 cut(s) 446, 513
MluCI AATT 3 cut(s) 143, 264, 585
MlyI GAGTC 2 cut(s) 145, 156
MnlI CCTC 7 cut(s) 91, 98, 235, 410, 527, 546, 568
Mva1269I GAATGC 1 cut(s) 33
MwoI GCNNNNNNNGC 3 cut(s) 320, 396, 664
NdeI CATATG 1 cut(s) 404
NdeII GATC 3 cut(s) 446, 513, 700
NlaIII CATG 5 cut(s) 97, 284, 551, 613, 680
NlaIV GGNNCC 2 cut(s) 448, 515
NmuCI GTSAC 5 cut(s) 110, 137, 231, 501, 739
PagI TCATGA 1 cut(s) 609
PceI AGGCCT 1 cut(s) 390
PctI GAATGC 1 cut(s) 33
PfeI GAWTC 2 cut(s) 484, 746
PkrI GCNGC 3 cut(s) 69, 322, 325
PleI GAGTC 2 cut(s) 144, 156
PpsI GAGTC 2 cut(s) 144, 156
PpuMI RGGWCCY 1 cut(s) 555
PsiI TTATAA 1 cut(s) 431
Psp5II RGGWCCY 1 cut(s) 555
PspN4I GGNNCC 2 cut(s) 448, 515
PspPI GGNCC 4 cut(s) 555, 579, 673, 690
PspPPI RGGWCCY 1 cut(s) 555
PstI CTGCAG 3 cut(s) 15, 499, 700
PstNI CAGNNNCTG 2 cut(s) 19, 743
PsuI RGATCY 2 cut(s) 446, 513
RsaI GTAC 1 cut(s) 647
RsaNI GTAC 1 cut(s) 646
SatI GCNGC 3 cut(s) 68, 321, 324
Sau3AI GATC 3 cut(s) 446, 513, 700
Sau96I GGNCC 4 cut(s) 555, 579, 673, 690
SchI GAGTC 2 cut(s) 145, 156
SetI ASST 5 cut(s) 80, 560, 576, 617, 627
SfaNI GCATC 2 cut(s) 192, 285
SfcI CTRYAG 4 cut(s) 11, 207, 495, 696
SfiI GGCCNNNNNGGCC 1 cut(s) 396
SinI GGWCC 2 cut(s) 555, 579
SmlI CTYRAG 2 cut(s) 84, 597
SmoI CTYRAG 2 cut(s) 84, 597
Sse9I AATT 3 cut(s) 143, 264, 585
SseBI AGGCCT 1 cut(s) 390
SsiI CCGC 1 cut(s) 68
StuI AGGCCT 1 cut(s) 390
StyI CCWWGG 1 cut(s) 450
TaqI TCGA 1 cut(s) 188
TasI AATT 3 cut(s) 143, 264, 585
TauI GCSGC 1 cut(s) 70
TfiI GAWTC 2 cut(s) 484, 746
TscAI CASTG 6 cut(s) 333, 504, 510, 598, 700, 748
TseFI GTSAC 5 cut(s) 110, 137, 231, 501, 739
TseI GCWGC 2 cut(s) 320, 323
Tsp45I GTSAC 5 cut(s) 110, 137, 231, 501, 739
TspDTI ATGAA 6 cut(s) 17, 110, 116, 269, 598, 693
TspRI CASTG 6 cut(s) 333, 504, 510, 598, 700, 748
VpaK11BI GGWCC 2 cut(s) 555, 579
XapI RAATTY 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.