Rw0G020200

Eukaryotic cytochrome b561

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00908
OG0008301
Physical Location & Seq
Forward (+)
6206 .. 7626
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G020200.1

Sequence Viewer

Length: 741 bp
ATGAGGTTTCTGCACAACTTGGGTTTCTTCTGTATTCTTTTTCTTGTTTCACCACTTTTCACTTCATCCCAAGAACATGTGAAAGGTGTAAGCAATCACAAAGAACACATTCAGAAGCACACGAGTCGCAAACTATCATTCGAAATTAAGCTTCATGGGTTTCTGCTTTGGGCTTCAATGGGGTTCTTGATGCCGGTAGGACTACTGGCCATTAGAATGTCACACAGAGAGCAATGTAGAAGAAGACTCAGAATTCTATTCTACGTTCATGCTTTATCAGAGATACTCTCTCTACTTCTTTCAACAGCAGGAGCTGTAATGTCCTTCAGAAACTTCTCCTTCAACAATAGGCACCAAAGGATTGGGGTTGGCCTCTATGGTCTGATATGGCTGCAAGCTCTAATTGGCATTGTAAGGCCACAAAGGGGGTCCAAAGGAAGAAGCATGTGGTTTTTGGTGCATTGGATGCTTGGAACCGCAATATCTCTGCTTGGGGTACTCAACATATACACAGGTTTAGTAGCCTACCAAGAGAAGACATCAAAAGGCATAAAGTTTTGGACCATAGTTTTCACAGCTGAAGTATGTTTCATTACCTTCTTCTACCTGTTCCAAGACAAATGGGTTTATATACAAAAGCAAGGAGTGAGTTTGGGTAATGAATCAAAAGGGGCCACCACAGATCAAGTGGTTTTGCCAAGAGACAAGCAAAAGGAAGTTGCCCCGGCAGAGTCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

246

Amino Acids

28.21

Weight (kDa)

10.1

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 51 - 174 2.8e-07 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 351
AciI CCGC 1 cut(s) 477
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 1 cut(s) 252
AcuI CTGAAG 2 cut(s) 310, 600
AfaI GTAC 1 cut(s) 498
AfiI CCNNNNNNNGG 1 cut(s) 425
AflIII ACRYGT 1 cut(s) 76
AgsI TTSAA 3 cut(s) 177, 303, 343
AluBI AGCT 4 cut(s) 151, 314, 398, 578
AluI AGCT 4 cut(s) 151, 314, 398, 578
Alw26I GTCTC 1 cut(s) 696
AlwNI CAGNNNCTG 1 cut(s) 314
AoxI GGCC 4 cut(s) 207, 370, 416, 672
ApeKI GCWGC 1 cut(s) 391
ApoI RAATTY 1 cut(s) 252
Asp700I GAANNNNTTC 1 cut(s) 108
AspS9I GGNCC 3 cut(s) 429, 561, 672
AsuC2I CCSGG 1 cut(s) 725
AsuHPI GGTGA 1 cut(s) 42
AsuII TTCGAA 1 cut(s) 141
AvaII GGWCC 2 cut(s) 429, 561
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 1 cut(s) 351
BauI CACGAG 1 cut(s) 121
BbsI GAAGAC 2 cut(s) 250, 542
BbvI GCAGC 1 cut(s) 378
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BcnI CCSGG 1 cut(s) 725
BcoDI GTCTC 1 cut(s) 696
BisI GCNGC 1 cut(s) 392
BlsI GCNGC 1 cut(s) 393
Bme1390I CCNGG 1 cut(s) 725
Bme18I GGWCC 2 cut(s) 429, 561
BmgT120I GGNCC 3 cut(s) 429, 561, 672
BmiI GGNNCC 4 cut(s) 353, 430, 475, 673
BmrFI CCNGG 1 cut(s) 725
BmsI GCATC 2 cut(s) 180, 456
BpiI GAAGAC 2 cut(s) 250, 542
BplI GAGNNNNNCTC 2 cut(s) 272, 304
Bpu14I TTCGAA 1 cut(s) 141
BpuMI CCSGG 1 cut(s) 725
BsaJI CCNNGG 1 cut(s) 723
Bsc4I CCNNNNNNNGG 1 cut(s) 425
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 210
Bse3DI GCAATG 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 723
BseGI GGATG 2 cut(s) 65, 471
BseLI CCNNNNNNNGG 1 cut(s) 425
BseMI GCAATG 1 cut(s) 239
BseMII CTCAG 1 cut(s) 262
BseNI ACTGG 1 cut(s) 210
BseXI GCAGC 1 cut(s) 378
BshFI GGCC 4 cut(s) 209, 372, 418, 674
BshNI GGYRCC 1 cut(s) 351
BsiSI CCGG 2 cut(s) 194, 725
BslI CCNNNNNNNGG 1 cut(s) 425
BsmAI GTCTC 1 cut(s) 696
BsnI GGCC 4 cut(s) 209, 372, 418, 674
Bsp119I TTCGAA 1 cut(s) 141
Bsp143I GATC 1 cut(s) 682
BspACI CCGC 1 cut(s) 477
BspANI GGCC 4 cut(s) 209, 372, 418, 674
BspCNI CTCAG 1 cut(s) 261
BspLI GGNNCC 4 cut(s) 353, 430, 475, 673
BspT104I TTCGAA 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 351
BsrDI GCAATG 1 cut(s) 239
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 1 cut(s) 210
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 1 cut(s) 723
BssMI GATC 1 cut(s) 682
BssSI CACGAG 1 cut(s) 121
Bst2BI CACGAG 1 cut(s) 121
BstAPI GCANNNNNTGC 1 cut(s) 466
BstBI TTCGAA 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 396
BstDEI CTNAG 1 cut(s) 248
BstF5I GGATG 2 cut(s) 65, 471
BstKTI GATC 1 cut(s) 685
BstMAI GTCTC 1 cut(s) 696
BstMBI GATC 1 cut(s) 682
BstMWI GCNNNNNNNGC 1 cut(s) 466
BstNSI RCATGY 2 cut(s) 80, 448
BstSCI CCNGG 1 cut(s) 723
BstV1I GCAGC 1 cut(s) 378
BstV2I GAAGAC 2 cut(s) 250, 542
BstXI CCANNNNNNTGG 1 cut(s) 362
BsuRI GGCC 4 cut(s) 209, 372, 418, 674
BtsCI GGATG 2 cut(s) 65, 471
Cac8I GCNNGC 1 cut(s) 396
CaiI CAGNNNCTG 1 cut(s) 314
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 3 cut(s) 429, 561, 672
Csp6I GTAC 1 cut(s) 497
CviAII CATG 4 cut(s) 77, 155, 269, 445
CviQI GTAC 1 cut(s) 497
DdeI CTNAG 1 cut(s) 248
DpnI GATC 1 cut(s) 684
DpnII GATC 1 cut(s) 682
EaeI YGGCCR 1 cut(s) 207
Eco47I GGWCC 2 cut(s) 429, 561
Eco57I CTGAAG 2 cut(s) 310, 600
EcoRI GAATTC 1 cut(s) 252
FaeI CATG 4 cut(s) 80, 158, 272, 448
FatI CATG 4 cut(s) 76, 154, 268, 444
Fnu4HI GCNGC 1 cut(s) 392
FokI GGATG 2 cut(s) 52, 478
Fsp4HI GCNGC 1 cut(s) 392
GluI GCNGC 1 cut(s) 392
HaeIII GGCC 4 cut(s) 209, 372, 418, 674
HapII CCGG 2 cut(s) 194, 725
Hin1II CATG 4 cut(s) 80, 158, 272, 448
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 4 cut(s) 124, 246, 662, 731
HpaII CCGG 2 cut(s) 194, 725
HphI GGTGA 1 cut(s) 42
Hpy188I TCNGA 5 cut(s) 114, 251, 280, 329, 384
Hpy188III TCNNGA 1 cut(s) 187
HpyAV CCTTC 3 cut(s) 334, 349, 607
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 3 cut(s) 13, 394, 460
HpyF10VI GCNNNNNNNGC 1 cut(s) 466
HpyF3I CTNAG 1 cut(s) 248
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 4 cut(s) 80, 158, 272, 448
Kzo9I GATC 1 cut(s) 682
LmnI GCTCC 1 cut(s) 311
LpnPI CCDG 5 cut(s) 191, 207, 294, 498, 620
Lsp1109I GCAGC 1 cut(s) 378
LweI GCATC 2 cut(s) 180, 456
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 1 cut(s) 219
MalI GATC 1 cut(s) 684
MboI GATC 1 cut(s) 682
MboII GAAGA 6 cut(s) 19, 252, 255, 450, 547, 592
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 3 cut(s) 144, 252, 402
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 3 cut(s) 133, 240, 740
MnlI CCTC 1 cut(s) 383
Mox20I TGGCCA 1 cut(s) 209
MroXI GAANNNNTTC 1 cut(s) 108
MscI TGGCCA 1 cut(s) 209
MseI TTAA 2 cut(s) 147, 739
MslI CAYNNNNRTG 1 cut(s) 215
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 578
MspI CCGG 2 cut(s) 194, 725
MspR9I CCNGG 1 cut(s) 725
MwoI GCNNNNNNNGC 1 cut(s) 466
NciI CCSGG 1 cut(s) 725
NdeII GATC 1 cut(s) 682
NlaIII CATG 4 cut(s) 80, 158, 272, 448
NlaIV GGNNCC 4 cut(s) 353, 430, 475, 673
NmuCI GTSAC 1 cut(s) 219
NspI RCATGY 2 cut(s) 80, 448
NspV TTCGAA 1 cut(s) 141
PciI ACATGT 1 cut(s) 76
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 1 cut(s) 662
PkrI GCNGC 1 cut(s) 393
PleI GAGTC 3 cut(s) 132, 240, 739
PpsI GAGTC 3 cut(s) 132, 240, 739
PscI ACATGT 1 cut(s) 76
PspN4I GGNNCC 4 cut(s) 353, 430, 475, 673
PspPI GGNCC 3 cut(s) 429, 561, 672
PstNI CAGNNNCTG 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 2 cut(s) 147, 739
SatI GCNGC 1 cut(s) 392
Sau3AI GATC 1 cut(s) 682
Sau96I GGNCC 3 cut(s) 429, 561, 672
SchI GAGTC 3 cut(s) 133, 240, 740
ScrFI CCNGG 1 cut(s) 725
SfaNI GCATC 2 cut(s) 180, 456
SfuI TTCGAA 1 cut(s) 141
SinI GGWCC 2 cut(s) 429, 561
SmiMI CAYNNNNRTG 1 cut(s) 215
Sse9I AATT 3 cut(s) 144, 252, 402
SsiI CCGC 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 723
TaiI ACGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 141
TasI AATT 3 cut(s) 144, 252, 402
TfiI GAWTC 1 cut(s) 662
Tru1I TTAA 2 cut(s) 147, 739
Tru9I TTAA 2 cut(s) 147, 739
TseFI GTSAC 1 cut(s) 219
TseI GCWGC 1 cut(s) 391
Tsp45I GTSAC 1 cut(s) 219
TspDTI ATGAA 5 cut(s) 54, 143, 257, 580, 675
VpaK11BI GGWCC 2 cut(s) 429, 561
XapI RAATTY 1 cut(s) 252
XceI RCATGY 2 cut(s) 80, 448
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XmnI GAANNNNTTC 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.