Rh2CG477200

Eukaryotic cytochrome b561

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0008301
Physical Location & Seq
Reverse (-)
64260301 .. 64261728
1428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG477200.1

Sequence Viewer

Length: 747 bp
ATGAGGTTTCTGCCCAACTTGGGTTTCTTCTGTATTCTTTTTCTTGTTTCACCACTTTTCACTTCATCCCAAGAACATGTGAAAGGTGTAAGCAATCACAAAGAACACATTCATAAGCACATGAGTCGCAAACTATCATTCGAAATTAAGCTTCATGGGTTTCTGCTTTGGGCTTCAATGGGGTTCTTGACGCCGGTAGGACTACTGGCCATTAGAATGTCACACAGAGTGCAATGTAGAAGAAGACTCAGAATTCTATTCTATGTTCATGCTTTATCAGAGATACTCTCTCTACTTCTTTCAACAGCAGGAGCTGTAATGTCCTTCAGAAACTTCAACAACTCCTTCAACAATAGGCACCAAAGGATTGGGGTTGGCCTCTATGGTCTGATATGGCTGCAAGCTCTAATTGGCATTGTAAGGCCACAAAGGGGGTCCAAAGGAAGAAGCATGTGGTTTTTGGTGCATTGGATGCTTGGAACTGCAATATCTCTGCTTGGGGTACTCAACATATACACAGGTTTAGTAGCCTACCAAGAGAAGACATCAAAAGGCATAAAGTTTTGGACCATAGTTTTCACAGCTGAAGTATGTTTCATTACCTTCTTCTACCTGTTCCAAGACAAATGGGTTTATATACATAAGCAAGGAGTGAGTTTGGGTAATGAATCAAAAGGGGCCACCACAGATCAAGTGGTTTTGCCAAGAGACAAGCAAAAGGAAGTTGCCCCGGCAGAGTCTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

248

Amino Acids

28.39

Weight (kDa)

10.18

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 51 - 176 1.6e-08 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 357
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 1 cut(s) 252
AcuI CTGAAG 2 cut(s) 310, 606
AcyI GRCGYC 1 cut(s) 191
AdeI CACNNNGTG 1 cut(s) 229
AfaI GTAC 1 cut(s) 504
AfiI CCNNNNNNNGG 2 cut(s) 20, 431
AflIII ACRYGT 1 cut(s) 76
AgsI TTSAA 4 cut(s) 177, 303, 337, 349
AjuI GAANNNNNNNTTGG 2 cut(s) 8, 40
AluBI AGCT 4 cut(s) 151, 314, 404, 584
AluI AGCT 4 cut(s) 151, 314, 404, 584
Alw26I GTCTC 1 cut(s) 702
AlwNI CAGNNNCTG 1 cut(s) 314
AoxI GGCC 4 cut(s) 207, 376, 422, 678
ApeKI GCWGC 1 cut(s) 397
ApoI RAATTY 1 cut(s) 252
Asp700I GAANNNNTTC 1 cut(s) 108
AspS9I GGNCC 3 cut(s) 435, 567, 678
AsuC2I CCSGG 1 cut(s) 731
AsuHPI GGTGA 1 cut(s) 42
AsuII TTCGAA 1 cut(s) 141
AvaII GGWCC 2 cut(s) 435, 567
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 1 cut(s) 357
BbsI GAAGAC 2 cut(s) 250, 548
BbvI GCAGC 1 cut(s) 384
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BcnI CCSGG 1 cut(s) 731
BcoDI GTCTC 1 cut(s) 702
BisI GCNGC 1 cut(s) 398
BlsI GCNGC 1 cut(s) 399
Bme1390I CCNGG 1 cut(s) 731
Bme18I GGWCC 2 cut(s) 435, 567
BmgT120I GGNCC 3 cut(s) 435, 567, 678
BmiI GGNNCC 3 cut(s) 359, 436, 679
BmrFI CCNGG 1 cut(s) 731
BmsI GCATC 1 cut(s) 462
BpiI GAAGAC 2 cut(s) 250, 548
BplI GAGNNNNNCTC 2 cut(s) 272, 304
Bpu14I TTCGAA 1 cut(s) 141
BpuMI CCSGG 1 cut(s) 731
BsaHI GRCGYC 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 729
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 431
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 210
Bse3DI GCAATG 1 cut(s) 239
BseDI CCNNGG 1 cut(s) 729
BseGI GGATG 2 cut(s) 65, 477
BseLI CCNNNNNNNGG 2 cut(s) 20, 431
BseMI GCAATG 1 cut(s) 239
BseMII CTCAG 1 cut(s) 262
BseNI ACTGG 1 cut(s) 210
BseXI GCAGC 1 cut(s) 384
BshFI GGCC 4 cut(s) 209, 378, 424, 680
BshNI GGYRCC 1 cut(s) 357
BsiSI CCGG 2 cut(s) 194, 731
BslI CCNNNNNNNGG 2 cut(s) 20, 431
BsmAI GTCTC 1 cut(s) 702
BsnI GGCC 4 cut(s) 209, 378, 424, 680
Bsp119I TTCGAA 1 cut(s) 141
Bsp143I GATC 1 cut(s) 688
BspANI GGCC 4 cut(s) 209, 378, 424, 680
BspCNI CTCAG 1 cut(s) 261
BspLI GGNNCC 3 cut(s) 359, 436, 679
BspT104I TTCGAA 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 357
BsrDI GCAATG 1 cut(s) 239
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 1 cut(s) 210
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 1 cut(s) 729
BssMI GATC 1 cut(s) 688
BssNI GRCGYC 1 cut(s) 191
BstACI GRCGYC 1 cut(s) 191
BstAPI GCANNNNNTGC 1 cut(s) 472
BstBI TTCGAA 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 402
BstDEI CTNAG 1 cut(s) 248
BstF5I GGATG 2 cut(s) 65, 477
BstKTI GATC 1 cut(s) 691
BstMAI GTCTC 1 cut(s) 702
BstMBI GATC 1 cut(s) 688
BstMWI GCNNNNNNNGC 1 cut(s) 472
BstNSI RCATGY 2 cut(s) 80, 454
BstSCI CCNGG 1 cut(s) 729
BstV1I GCAGC 1 cut(s) 384
BstV2I GAAGAC 2 cut(s) 250, 548
BstXI CCANNNNNNTGG 1 cut(s) 368
BsuRI GGCC 4 cut(s) 209, 378, 424, 680
BtsCI GGATG 2 cut(s) 65, 477
Cac8I GCNNGC 1 cut(s) 402
CaiI CAGNNNCTG 1 cut(s) 314
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 3 cut(s) 435, 567, 678
CseI GACGC 1 cut(s) 199
Csp6I GTAC 1 cut(s) 503
CviAII CATG 5 cut(s) 77, 121, 155, 269, 451
CviQI GTAC 1 cut(s) 503
DdeI CTNAG 1 cut(s) 248
DpnI GATC 1 cut(s) 690
DpnII GATC 1 cut(s) 688
DraIII CACNNNGTG 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 207
Eco47I GGWCC 2 cut(s) 435, 567
Eco57I CTGAAG 2 cut(s) 310, 606
EcoRI GAATTC 1 cut(s) 252
FaeI CATG 5 cut(s) 80, 124, 158, 272, 454
FatI CATG 5 cut(s) 76, 120, 154, 268, 450
Fnu4HI GCNGC 1 cut(s) 398
FokI GGATG 2 cut(s) 52, 484
Fsp4HI GCNGC 1 cut(s) 398
GluI GCNGC 1 cut(s) 398
HaeIII GGCC 4 cut(s) 209, 378, 424, 680
HapII CCGG 2 cut(s) 194, 731
HgaI GACGC 1 cut(s) 199
Hin1I GRCGYC 1 cut(s) 191
Hin1II CATG 5 cut(s) 80, 124, 158, 272, 454
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 4 cut(s) 124, 246, 668, 737
HpaII CCGG 2 cut(s) 194, 731
HphI GGTGA 1 cut(s) 42
Hpy188I TCNGA 4 cut(s) 251, 280, 329, 390
Hpy188III TCNNGA 1 cut(s) 187
HpyAV CCTTC 3 cut(s) 334, 355, 613
HpyCH4V TGCA 4 cut(s) 232, 400, 466, 485
HpyF10VI GCNNNNNNNGC 1 cut(s) 472
HpyF3I CTNAG 1 cut(s) 248
Hsp92I GRCGYC 1 cut(s) 191
Hsp92II CATG 5 cut(s) 80, 124, 158, 272, 454
Kzo9I GATC 1 cut(s) 688
LmnI GCTCC 1 cut(s) 311
LpnPI CCDG 5 cut(s) 191, 207, 294, 504, 626
Lsp1109I GCAGC 1 cut(s) 384
LweI GCATC 1 cut(s) 462
MaeIII GTNAC 1 cut(s) 219
MalI GATC 1 cut(s) 690
MboI GATC 1 cut(s) 688
MboII GAAGA 6 cut(s) 19, 252, 255, 456, 553, 598
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 3 cut(s) 144, 252, 408
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 3 cut(s) 133, 240, 746
MnlI CCTC 1 cut(s) 389
Mox20I TGGCCA 1 cut(s) 209
MroXI GAANNNNTTC 1 cut(s) 108
MscI TGGCCA 1 cut(s) 209
MseI TTAA 2 cut(s) 147, 745
MslI CAYNNNNRTG 1 cut(s) 215
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 2 cut(s) 194, 731
MspR9I CCNGG 1 cut(s) 731
MwoI GCNNNNNNNGC 1 cut(s) 472
NciI CCSGG 1 cut(s) 731
NdeII GATC 1 cut(s) 688
NlaIII CATG 5 cut(s) 80, 124, 158, 272, 454
NlaIV GGNNCC 3 cut(s) 359, 436, 679
NmuCI GTSAC 1 cut(s) 219
NspI RCATGY 2 cut(s) 80, 454
NspV TTCGAA 1 cut(s) 141
PciI ACATGT 1 cut(s) 76
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 1 cut(s) 668
PkrI GCNGC 1 cut(s) 399
PleI GAGTC 3 cut(s) 132, 240, 745
PpsI GAGTC 3 cut(s) 132, 240, 745
PscI ACATGT 1 cut(s) 76
PspN4I GGNNCC 3 cut(s) 359, 436, 679
PspPI GGNCC 3 cut(s) 435, 567, 678
PstNI CAGNNNCTG 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 584
RsaI GTAC 1 cut(s) 504
RsaNI GTAC 1 cut(s) 503
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 2 cut(s) 147, 745
SatI GCNGC 1 cut(s) 398
Sau3AI GATC 1 cut(s) 688
Sau96I GGNCC 3 cut(s) 435, 567, 678
SchI GAGTC 3 cut(s) 133, 240, 746
ScrFI CCNGG 1 cut(s) 731
SetI ASST 9 cut(s) 8, 88, 153, 316, 406, 523, 586, 605, 615
SfaNI GCATC 1 cut(s) 462
SfuI TTCGAA 1 cut(s) 141
SinI GGWCC 2 cut(s) 435, 567
SmiMI CAYNNNNRTG 1 cut(s) 215
Sse9I AATT 3 cut(s) 144, 252, 408
StyD4I CCNGG 1 cut(s) 729
TaqI TCGA 1 cut(s) 141
TasI AATT 3 cut(s) 144, 252, 408
TfiI GAWTC 1 cut(s) 668
Tru1I TTAA 2 cut(s) 147, 745
Tru9I TTAA 2 cut(s) 147, 745
TseFI GTSAC 1 cut(s) 219
TseI GCWGC 1 cut(s) 397
Tsp45I GTSAC 1 cut(s) 219
TspDTI ATGAA 6 cut(s) 54, 101, 143, 257, 586, 681
VpaK11BI GGWCC 2 cut(s) 435, 567
XapI RAATTY 1 cut(s) 252
XceI RCATGY 2 cut(s) 80, 454
XcmI CCANNNNNNNNNTGG 1 cut(s) 691
XmnI GAANNNNTTC 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.