MD17G1136300.v1.1

Eukaryotic cytochrome b561

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008301
Physical Location & Seq
Forward (+)
12217753 .. 12219881
2129 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1136300.v1.1.491

Sequence Viewer

Length: 759 bp
ATGGGGTTTCTACAGAAATTGGGTTTCTTCTGCATTCGTTTAAGTTTTCTTCTTTTCCTTGTTTCGCCGCTCGTAAGCTCGTCCCAAGAACAAATGAAAGGTGCAGGCAGTCACAAAGAGAACATTCATAAGCAGATGAGTCACAAACTGTCATCGGAAATCAGACTCCACGGGTTTCTTCTTTGGGCTTCAATGGGGTTCTTGATGCCTGTAGGAATACTTGCTATCAGAATGTCACACGGAGAGGAATGTGGAAGAAGGCTCAGAATTCTTTTCTATGTTCATGGGTTATCAGAGATGCTCTCTGTCCTTCTTGCAACAGCAGGAGCAGTGATGTCCTTCAGAAACTTCAACAACTCCTTCAACAATAAACACCAAAGGGTTGGGATAGGCTTATATGGCCTAATATGGTTGCAGGCCCTAATTGGGTTTGTAAGGCCACAGAGGGGATCCAAGGGAAGAAGCATATGGTTTTCTGTACATTGGATACTTGGAACTGCAGTGTCTCTGCTTGGGGTCCTCAACATATACACAGGACTACAAGCCTACCACGAGAAGACCTCAAAAGGCATAAAGCTTTGGACCATAATTTTCACTGCTCAAGTCAGTGCCATCACCTTCTTCTACCTGTTCCAAGACAAATGGTTGTACATCCAAAAGCAAGGAGTGCTTCTGGGCAATGAACCAGTACGGCCCACCGCGGATCAAGTGCTTTTGCCAATTGAAAAGCAAAAGCAGTCGGTGACTGATTCTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

253

Amino Acids

28.45

Weight (kDa)

10.09

Isoelectric Point (pI)

57.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 56 - 181 6.4e-09 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 70
AccII CGCG 1 cut(s) 701
AciI CCGC 3 cut(s) 68, 699, 701
AclWI GGATC 3 cut(s) 444, 457, 711
AcsI RAATTY 1 cut(s) 267
AcuI CTGAAG 1 cut(s) 325
AfaI GTAC 3 cut(s) 480, 650, 690
AfiI CCNNNNNNNGG 2 cut(s) 426, 446
AgsI TTSAA 4 cut(s) 192, 352, 364, 725
AluBI AGCT 3 cut(s) 78, 577, 756
AluI AGCT 3 cut(s) 78, 577, 756
Alw26I GTCTC 1 cut(s) 510
AlwI GGATC 3 cut(s) 444, 457, 711
AoxI GGCC 4 cut(s) 400, 417, 437, 692
ApoI RAATTY 1 cut(s) 267
AspS9I GGNCC 4 cut(s) 418, 517, 582, 693
AsuHPI GGTGA 2 cut(s) 607, 754
AvaII GGWCC 2 cut(s) 517, 582
BamHI GGATCC 1 cut(s) 449
BauI CACGAG 1 cut(s) 551
BbsI GAAGAC 1 cut(s) 563
BccI CCATC 1 cut(s) 620
BceAI ACGGC 1 cut(s) 707
BciVI GTATCC 1 cut(s) 480
BcoDI GTCTC 1 cut(s) 510
BfaI CTAG 1 cut(s) 753
BfmI CTRYAG 3 cut(s) 11, 210, 498
BfuI GTATCC 1 cut(s) 480
BisI GCNGC 1 cut(s) 68
BlsI GCNGC 1 cut(s) 69
Bme18I GGWCC 2 cut(s) 517, 582
BmgT120I GGNCC 4 cut(s) 418, 517, 582, 693
BmiI GGNNCC 2 cut(s) 451, 518
BmsI GCATC 2 cut(s) 195, 288
BpiI GAAGAC 1 cut(s) 563
BplI GAGNNNNNCTC 4 cut(s) 287, 319, 545, 577
BpuEI CTTGAG 1 cut(s) 585
BsaJI CCNNGG 3 cut(s) 169, 453, 699
Bsc4I CCNNNNNNNGG 2 cut(s) 426, 446
Bse1I ACTGG 1 cut(s) 686
Bse3DI GCAATG 1 cut(s) 685
BseDI CCNNGG 3 cut(s) 169, 453, 699
BseGI GGATG 1 cut(s) 651
BseLI CCNNNNNNNGG 2 cut(s) 426, 446
BseMI GCAATG 1 cut(s) 685
BseMII CTCAG 1 cut(s) 277
BseNI ACTGG 1 cut(s) 686
BsgI GTGCAG 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 701
BshFI GGCC 4 cut(s) 402, 419, 439, 694
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 2 cut(s) 426, 446
BsmAI GTCTC 1 cut(s) 510
BsmFI GGGAC 1 cut(s) 67
BsmI GAATGC 1 cut(s) 33
BsnI GGCC 4 cut(s) 402, 419, 439, 694
Bsp1407I TGTACA 2 cut(s) 478, 648
Bsp143I GATC 2 cut(s) 449, 703
BspACI CCGC 3 cut(s) 68, 699, 701
BspANI GGCC 4 cut(s) 402, 419, 439, 694
BspCNI CTCAG 1 cut(s) 276
BspFNI CGCG 1 cut(s) 701
BspLI GGNNCC 2 cut(s) 451, 518
BspMAI CTGCAG 1 cut(s) 502
BspPI GGATC 3 cut(s) 444, 457, 711
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 1 cut(s) 685
BsrGI TGTACA 2 cut(s) 478, 648
BsrI ACTGG 1 cut(s) 686
BssECI CCNNGG 3 cut(s) 169, 453, 699
BssMI GATC 2 cut(s) 449, 703
BssSI CACGAG 1 cut(s) 551
BssT1I CCWWGG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 551
Bst4CI ACNGT 1 cut(s) 150
BstAPI GCANNNNNTGC 1 cut(s) 667
BstAUI TGTACA 2 cut(s) 478, 648
BstC8I GCNNGC 2 cut(s) 106, 417
BstDEI CTNAG 1 cut(s) 263
BstDSI CCRYGG 2 cut(s) 169, 699
BstF5I GGATG 1 cut(s) 651
BstFNI CGCG 1 cut(s) 701
BstKTI GATC 2 cut(s) 452, 706
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 2 cut(s) 449, 703
BstMWI GCNNNNNNNGC 2 cut(s) 399, 667
BstSFI CTRYAG 3 cut(s) 11, 210, 498
BstUI CGCG 1 cut(s) 701
BstV2I GAAGAC 1 cut(s) 563
BstX2I RGATCY 1 cut(s) 449
BstXI CCANNNNNNTGG 1 cut(s) 383
BstYI RGATCY 1 cut(s) 449
BsuI GTATCC 1 cut(s) 480
BsuRI GGCC 4 cut(s) 402, 419, 439, 694
BtgI CCRYGG 2 cut(s) 169, 699
BtsCI GGATG 1 cut(s) 651
BtsI GCAGTG 3 cut(s) 336, 507, 594
BtsIMutI CAGTG 4 cut(s) 336, 507, 594, 613
Cac8I GCNNGC 2 cut(s) 106, 417
Cfr13I GGNCC 4 cut(s) 418, 517, 582, 693
Cfr42I CCGCGG 1 cut(s) 702
Csp6I GTAC 3 cut(s) 479, 649, 689
CviAII CATG 1 cut(s) 284
CviQI GTAC 3 cut(s) 479, 649, 689
DdeI CTNAG 1 cut(s) 263
DpnI GATC 2 cut(s) 451, 705
DpnII GATC 2 cut(s) 449, 703
Eco130I CCWWGG 1 cut(s) 453
Eco47I GGWCC 2 cut(s) 517, 582
Eco57I CTGAAG 1 cut(s) 325
EcoO109I RGGNCCY 2 cut(s) 418, 517
EcoRI GAATTC 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 1 cut(s) 287
FalI AAGNNNNNCTT 2 cut(s) 654, 686
FaqI GGGAC 1 cut(s) 67
FatI CATG 1 cut(s) 283
FauNDI CATATG 1 cut(s) 467
Fnu4HI GCNGC 1 cut(s) 68
FokI GGATG 1 cut(s) 638
Fsp4HI GCNGC 1 cut(s) 68
FspBI CTAG 1 cut(s) 753
GluI GCNGC 1 cut(s) 68
HaeIII GGCC 4 cut(s) 402, 419, 439, 694
Hin1II CATG 1 cut(s) 287
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 3 cut(s) 139, 165, 749
HphI GGTGA 2 cut(s) 607, 754
Hpy188I TCNGA 6 cut(s) 157, 164, 230, 266, 295, 344
Hpy188III TCNNGA 1 cut(s) 202
HpyAV CCTTC 5 cut(s) 252, 320, 349, 370, 628
HpyCH4III ACNGT 1 cut(s) 150
HpyCH4V TGCA 5 cut(s) 33, 104, 317, 415, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 399, 667
HpyF3I CTNAG 1 cut(s) 263
Hsp92II CATG 1 cut(s) 287
KspI CCGCGG 1 cut(s) 702
Kzo9I GATC 2 cut(s) 449, 703
LmnI GCTCC 1 cut(s) 326
LpnPI CCDG 8 cut(s) 90, 222, 309, 401, 519, 641, 659, 699
LweI GCATC 2 cut(s) 195, 288
MaeI CTAG 1 cut(s) 753
MaeIII GTNAC 4 cut(s) 110, 140, 234, 742
MalI GATC 2 cut(s) 451, 705
MbiI CCGCTC 1 cut(s) 70
MboI GATC 2 cut(s) 449, 703
MboII GAAGA 7 cut(s) 19, 41, 170, 267, 471, 568, 613
MfeI CAATTG 1 cut(s) 720
MflI RGATCY 1 cut(s) 449
MluCI AATT 5 cut(s) 17, 267, 423, 588, 720
MlyI GAGTC 2 cut(s) 148, 159
MnlI CCTC 4 cut(s) 238, 438, 530, 571
MseI TTAA 1 cut(s) 41
MspA1I CMGCKG 1 cut(s) 701
MunI CAATTG 1 cut(s) 720
Mva1269I GAATGC 1 cut(s) 33
MvnI CGCG 1 cut(s) 701
MwoI GCNNNNNNNGC 2 cut(s) 399, 667
NdeI CATATG 1 cut(s) 467
NdeII GATC 2 cut(s) 449, 703
NlaIII CATG 1 cut(s) 287
NlaIV GGNNCC 2 cut(s) 451, 518
NmuCI GTSAC 4 cut(s) 110, 140, 234, 742
PctI GAATGC 1 cut(s) 33
PfeI GAWTC 1 cut(s) 749
PkrI GCNGC 1 cut(s) 69
PleI GAGTC 2 cut(s) 147, 159
PpsI GAGTC 2 cut(s) 147, 159
PpuMI RGGWCCY 1 cut(s) 517
Psp5II RGGWCCY 1 cut(s) 517
PspN4I GGNNCC 2 cut(s) 451, 518
PspPI GGNCC 4 cut(s) 418, 517, 582, 693
PspPPI RGGWCCY 1 cut(s) 517
PstI CTGCAG 1 cut(s) 502
PsuI RGATCY 1 cut(s) 449
RsaI GTAC 3 cut(s) 480, 650, 690
RsaNI GTAC 3 cut(s) 479, 649, 689
SacII CCGCGG 1 cut(s) 702
SaqAI TTAA 1 cut(s) 41
SatI GCNGC 1 cut(s) 68
Sau3AI GATC 2 cut(s) 449, 703
Sau96I GGNCC 4 cut(s) 418, 517, 582, 693
SchI GAGTC 2 cut(s) 148, 159
SetI ASST 7 cut(s) 80, 103, 563, 579, 620, 630, 758
SfaNI GCATC 2 cut(s) 195, 288
SfcI CTRYAG 3 cut(s) 11, 210, 498
Sfr303I CCGCGG 1 cut(s) 702
SgrBI CCGCGG 1 cut(s) 702
SinI GGWCC 2 cut(s) 517, 582
SmlI CTYRAG 1 cut(s) 600
SmoI CTYRAG 1 cut(s) 600
Sse9I AATT 5 cut(s) 17, 267, 423, 588, 720
SsiI CCGC 3 cut(s) 68, 699, 701
SspMI CTAG 1 cut(s) 753
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 1 cut(s) 150
TasI AATT 5 cut(s) 17, 267, 423, 588, 720
TatI WGTACW 2 cut(s) 478, 648
TauI GCSGC 1 cut(s) 70
TfiI GAWTC 1 cut(s) 749
Tru1I TTAA 1 cut(s) 41
Tru9I TTAA 1 cut(s) 41
TscAI CASTG 4 cut(s) 336, 507, 601, 613
TseFI GTSAC 4 cut(s) 110, 140, 234, 742
Tsp45I GTSAC 4 cut(s) 110, 140, 234, 742
TspDTI ATGAA 4 cut(s) 110, 116, 272, 696
TspGWI ACGGA 1 cut(s) 255
TspRI CASTG 4 cut(s) 336, 507, 601, 613
VpaK11BI GGWCC 2 cut(s) 517, 582
XapI RAATTY 1 cut(s) 267
XspI CTAG 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.