Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008228

Physical Location & Seq

Genomic Coordinates Reverse (-)

12885078 .. 12894739

Gene Structure 9662 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1160300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3303 bp

ATGGCTGCGTCACGTGGTTCCTCGTCTTCCACGAACGGGTTGTTGCCAACGTCGTTGTCGGAGCAGAACCAACACGACATGGAGGCAGGGGGAGGAGGGGCGAGGGTGGGACTGGAGACCGAGTTCGACTACGACGCTGCCGCGGCGAACGACATGCCGACGGATCCCTTCGACATTGCCCACACCAAGAATGCGCCTCACGAGACGCTCAGGAGATGGAGGCAAGCGGCATTAGTGCTAAATGCTTCCAGACGGTTCAGGTACACTATGGACTTGAAAGCGGAAGAAGAAAAAGAAAAAAGGAGGCGGATGATTAGATCCCATGCACAAGTCATACGAGCAGCATTGCTTTTCAAATTGGCAGGAGAACGGGAACTTGGGATAGGCACAACAGTTGCGCCCCCAACTCCCAGTGGTGATTACGGAATTGCACTAGACCAACTTGTTACATTGACCAGGGAGAACAATATTACTGCTTTACAGCAATATGGAGGGATTAAAGGCATATCAGCGTTGCTAAAATCGAACGTGGAGAAGGGAATTCGTGGAGATGAAACAGATGTAGTAAAACGAAAGGAAGTGTTTGGATCAAATACATATCCTCGGAAAAAAGGGCGGAGTTTCTGGAGGTTCCTTTGGGAAGCTTGGCAAGACTTGACTCTTATAATCTTGATCATCGCAGCAGTGGTGTCATTGGCACTCGGATTAAAAACTGAGGGTTTGGAGGAAGGATGGTATGACGGAGCAAGCATTTTCTTTGCTGTGTTTCTTGTTATAGTTGTCACAGCTGTTAGTGATTATCGGCAATCTCTTCAGTTCCAAAATCTAAACGCGGAAAAACAAAATATACAATTGGAGGTCATGAGAGGCGGTAGATCAGTAACGATTTCAATATTTGATATTGTGGTTGGTGATGTTGTACCTCTCAGAATAGGTGATCAAGTCCCTGCTGATGGAATCGTAATCACTGGTCATTCACTTGCTATTGATGAATCTAGCATGACTGGTGAAAGCAAGATTGTTCACAAGGATCAGAAAGCACCTTTCTTAATGTCAGGTTGCAAAGTGGCTGATGGAGTGGGTACTATGCTGGTAACTGGTGTTGGAATCAATACTGAATGGGGATTATTGATGGCGAGCATCTCAGAGGATACTGGTGAAGAAACTCCTTTGCAGGTGCGGTTGAATGGAGTTGCAACTTTTATAGGCATAGTTGGGCTTTCAGTGGCTGCTTTGGTGCTAGCCGTCCTTTGGGGCAGATACTTTACCGGAAATACAAAAGATCTAGAGGGAAACGTACAGTTTAAAGCTGGTCAGACTAGCATTAGCAAAACCTTTGATGGAGCAGTGAAAATTTTCACCATTGCTGTCACCATTGTGGTTGTTGCAGTGCCTGAAGGGCTACCTTTGGCCGTTACCTTGACGCTAGCATACTCAATGAAGAAAATGATGGCAGATAAGGCATTGGTGCGTAGACTGTCAGCGTGTGAAACTATGGGCTCAGCAACAACAATTTGCAGTGATAAAACTGGGACATTGACTTTAAATCAGATGACTGTGGTTGAGGCATATGTTGGGAGAAAAAAGATAAATCCGCCAGATGATACCTCACAGTTGCATCCGCTGGTTTCCACTCTCCTAAGTGAGGGCGTTGCGCACAATACCACTGGCAACGTATTTGAGCCAACGGGTGGTAAAGTCGAGATTTCTGGATCTCCTACGGAAAAGGCTATACTTTCATGGGCATTCAAGTTGGGGATGAAGTTTGATGTTATCAAATTGGAGTCTACAGTCCTTCATGTTTTCCCTTTCAACTCGGAGAAAAAGCGAGGTGGTGTTGCACTAAAACAGGCTGACTCTAAAGTTCATATACATTGGAAAGGAGCAGCGGAGATAGTTCTAGCCTCGTGCACAGAATATCTTGACTCAGATGGTTGCTCGCAGAATATTGAAGATGACAAGGAGTTTTTCAAGACGGCTATTGATGACATGGCAGAACGCAGTTTGCGGTGTGTTGCCATTGCATACAGACCATATGAACAGGACAAAGTTCCTACTGATGAAGAGCAATTAAGTCAATGGGCTTTACCAGAAGATAACCTTGTCCTGCTTGCTATTGTTGGTATAAAGGATCCTTGTCGTCCTGGTGTCAAAGATGCAGTGCAACTATGCACTGAAGCTGGTGTTAAGGTACGCATGGTCACTGGAGACAATCTTCAAACAGCAAAAGCCATCGCGTTAGAGTGTGGAATACTACTTTCACTCGAAGATGCAACTGAGCCTACTATCATCGAAGGAAAAACATTCCGTGAATTGTCTGAAAAGGAAAGGGAACAAACCGCCAAGAAAATAACAGTAATGGGCAGATCTTCCCCTAATGACAAGTTGTTGCTTGTGCAAGCATTACGTAAGGGGGGTGAAGTTGTTGCAGTAACAGGAGATGGCACTAATGATGCTCCAGCACTTCATGAGGCAGATATTGGTCTTTCTATGGGCATTCAAGGAACTGAAGTTGCAAAAGAAAGTTCGGATATCGTTATCTTGGATGACAATTTTGCCTCTGTTGTAAAGGTTGTTCGCTGGGGACGTTCTGTGTATGCAAATATTCAGAAATTTATCCAATTTCAACTTACTGTTAATGTTGCCGCTCTTGTAATTAATGTTGTGGCTGCAATCTCTTCTGGTAGTGTTCCGTTAAATGCAGTACAGCTTCTGTGGGTTAACTTGATCATGGATACTCTAGGAGCACTTGCATTGGCTACCGAACCACCTACAGACAACCTTATGCAGAGAACACCAGTTGGCCGGAGGGCACCTCTTATAACGAACATCATGTGGAGGAACCTACTCGTTCAGGCTATGTATCAAGTTACCGTCCTCCTTGTCCTCAACTTTAAGGGAAATCGCATTCTTGGTCTGCAGAAGGATGTCCAAAAGCATGCCATTGGTGTGAAGAATACTCTCATATTCAATGCATTCGTCTTTTGCCAAATTTTCAATGAGTTCAATGCTCGAAAACCTGAAGAAATAAATGTGTTCATCGGAGTGACCAAAAACTACCTCTTCATGGGAATAATAGGAATCACATTGGTACTTCAGATAATCATCGTTATGTTCCTCGGAAAATTTACAAAAACAGTGAGACTATCATGGCAGCAATGGCTCATATGCTTGGGAATTGCCATTGTCAGTTGGCCTCTTGCTGTTATTGGAAAACTAATTCCGGTCCCAAAGACTCCGTTTTCCCAGTACTTCAGTCGGCCTATTCAGCGGTACAGAAATTCTCGTAATCGTAATACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007275 GO:0007338 GO:0008150 GO:0008324 GO:0009555 GO:0009566 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019953 GO:0022414 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044703 GO:0046873 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0051704 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1101

Amino Acids

120.23

Weight (kDa)

7.54

Isoelectric Point (pI)

35.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	55 - 97	4.3e-16	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	163 - 231	1.7e-15	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	284 - 380	4.5e-19	P-type ATPase actuator domain
Hydrolase	PF00702	504 - 826	5.3e-16	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	544 - 715	1.1e-30	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	897 - 1074	9.2e-45	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008228)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21180
fragaria_vesca	FvH4_6g37190 FvH4_6g37190 FvH4_6g37190 FvH4_6g37190
malus_domestica	MD09G1160300.v1.1 MD17G1146100.v1.1
prunus_persica	Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1
pyrus_communis	pycom09g07890 pycom17g13890
rosa_chinensis	RchiOBHm_Chr2g0149841
rosa_laevigata	RLG00000020450
rosa_multiflora	Rmu_ssc0000308.1_g000029
rosa_roxburghii	Rroxscaffold_2G00097600
rosa_rugosa	Rorug02G0417000
rosa_samantha	Rh2BG487700 Rh2CG461600 Rh2DG496500
rosa_wichuraiana	Rw2G038820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	665, 2822
AarI	CACCTGC	1 cut(s)	1165
Acc16I	TGCGCA	1 cut(s)	1656
Acc36I	ACCTGC	1 cut(s)	1165
AccB1I	GGYRCC	1 cut(s)	2812
AccB7I	CCANNNNNTGG	1 cut(s)	1691
AccBSI	CCGCTC	1 cut(s)	2648
AccI	GTMKAC	2 cut(s)	1474, 1787
AccII	CGCG	3 cut(s)	143, 833, 2237
AclWI	GGATC	8 cut(s)	158, 171, 312, 595, 1038, 1720, 2126, 2139
AcoI	YGGCCR	2 cut(s)	1410, 2803
AcsI	RAATTY	6 cut(s)	540, 1353, 2612, 2991, 3125, 3280
AcuI	CTGAAG	7 cut(s)	797, 1416, 2196, 2529, 3042, 3080, 3238
AcvI	CACGTG	1 cut(s)	14
AfaI	GTAC	9 cut(s)	263, 921, 1084, 1299, 2193, 2706, 3093, 3251, 3275
AfiI	CCNNNNNNNGG	6 cut(s)	36, 953, 1251, 1645, 1691, 3067
AjnI	CCWGG	2 cut(s)	455, 2143
AjuI	GAANNNNNNNTTGG	2 cut(s)	360, 392
AleI	CACNNNNGTG	1 cut(s)	1376
AloI	GAACNNNNNNTCC	2 cut(s)	107, 139
AluBI	AGCT	5 cut(s)	644, 788, 1310, 2180, 2710
AluI	AGCT	5 cut(s)	644, 788, 1310, 2180, 2710
Alw21I	GWGCWC	2 cut(s)	1913, 2749
Alw26I	GTCTC	4 cut(s)	110, 197, 2202, 3136
Alw44I	GTGCAC	1 cut(s)	1909
AlwI	GGATC	8 cut(s)	158, 171, 312, 595, 1038, 1720, 2126, 2139
AlwNI	CAGNNNCTG	3 cut(s)	1229, 1394, 2713
AoxI	GGCC	4 cut(s)	1410, 2803, 3194, 3260
ApaLI	GTGCAC	1 cut(s)	1909
ApeKI	GCWGC	8 cut(s)	5, 137, 341, 680, 1229, 1886, 2669, 3154
ApoI	RAATTY	6 cut(s)	540, 1353, 2612, 2991, 3125, 3280
AseI	ATTAAT	1 cut(s)	2658
Asp700I	GAANNNNTTC	1 cut(s)	1355
AspLEI	GCGC	3 cut(s)	196, 400, 1657
AspS9I	GGNCC	1 cut(s)	3226
AsuHPI	GGTGA	8 cut(s)	428, 923, 947, 1019, 1169, 1351, 1363, 2429
AsuNHI	GCTAGC	2 cut(s)	1240, 1426
AvaII	GGWCC	1 cut(s)	3226
BaeGI	GKGCMC	2 cut(s)	1913, 2815
BamHI	GGATCC	2 cut(s)	163, 2131
BanI	GGYRCC	1 cut(s)	2812
BanII	GRGCYC	1 cut(s)	1502
BauI	CACGAG	2 cut(s)	200, 1906
BbrPI	CACGTG	1 cut(s)	14
BbsI	GAAGAC	1 cut(s)	18
Bbv12I	GWGCWC	2 cut(s)	1913, 2749
BbvI	GCAGC	7 cut(s)	124, 353, 692, 1216, 1898, 2656, 3166
BceAI	ACGGC	3 cut(s)	1229, 1397, 1992
BcgI	CGANNNNNNTGC	2 cut(s)	136, 170
BciT130I	CCWGG	2 cut(s)	457, 2145
BciVI	GTATCC	2 cut(s)	1144, 2728
BclI	TGATCA	3 cut(s)	672, 937, 2727
BcoDI	GTCTC	4 cut(s)	110, 197, 2202, 3136
BfaI	CTAG	8 cut(s)	434, 996, 1241, 1286, 1320, 1427, 1901, 2741
BfmI	CTRYAG	3 cut(s)	1788, 2772, 2918
BfuAI	ACCTGC	1 cut(s)	1165
BfuI	GTATCC	2 cut(s)	1144, 2728
BglI	GCCNNNNNGGC	1 cut(s)	1399
BglII	AGATCT	2 cut(s)	1282, 2366
BlpI	GCTNAGC	1 cut(s)	1501
BmcAI	AGTACT	1 cut(s)	3251
Bme1390I	CCNGG	2 cut(s)	457, 2145
Bme18I	GGWCC	1 cut(s)	3226
BmgT120I	GGNCC	1 cut(s)	3226
BmiI	GGNNCC	7 cut(s)	19, 165, 632, 2133, 2814, 2843, 3228
BmrFI	CCNGG	2 cut(s)	457, 2145
BmrI	ACTGGG	3 cut(s)	405, 1539, 3241
BmsI	GCATC	5 cut(s)	1149, 1627, 2146, 2260, 2443
BmtI	GCTAGC	2 cut(s)	1244, 1430
BmuI	ACTGGG	3 cut(s)	405, 1539, 3241
BpiI	GAAGAC	1 cut(s)	18
BplI	GAGNNNNNCTC	2 cut(s)	2800, 2832
BpmI	CTGGAG	4 cut(s)	134, 646, 2226, 2442
Bpu10I	CCTNAGC	1 cut(s)	209
Bpu1102I	GCTNAGC	1 cut(s)	1501
BsaAI	YACGTR	2 cut(s)	14, 2408
BsaBI	GATNNNNATC	1 cut(s)	3104
BsaI	GGTCTC	1 cut(s)	110
BsaJI	CCNNGG	4 cut(s)	141, 456, 602, 3118
BsaWI	WCCGGW	2 cut(s)	1268, 3223
BsaXI	ACNNNNNCTCC	2 cut(s)	107, 137
Bsc4I	CCNNNNNNNGG	6 cut(s)	36, 953, 1251, 1645, 1691, 3067
Bse3DI	GCAATG	5 cut(s)	174, 344, 1362, 2019, 3164
Bse8I	GATNNNNATC	1 cut(s)	3104
BseBI	CCWGG	2 cut(s)	457, 2145
BseDI	CCNNGG	4 cut(s)	141, 456, 602, 3118
BseGI	GGATG	6 cut(s)	315, 737, 1618, 1764, 2551, 2932
BseJI	GATNNNNATC	1 cut(s)	3104
BseLI	CCNNNNNNNGG	6 cut(s)	36, 953, 1251, 1645, 1691, 3067
BseMI	GCAATG	5 cut(s)	174, 344, 1362, 2019, 3164
BseMII	CTCAG	7 cut(s)	223, 705, 940, 1158, 1515, 1941, 2268
BseRI	GAGGAG	1 cut(s)	108
BseSI	GKGCMC	2 cut(s)	1913, 2815
BseXI	GCAGC	7 cut(s)	124, 353, 692, 1216, 1898, 2656, 3166
BseYI	CCCAGC	1 cut(s)	2580
Bsh1236I	CGCG	3 cut(s)	143, 833, 2237
BshFI	GGCC	4 cut(s)	1412, 2805, 3196, 3262
BshNI	GGYRCC	1 cut(s)	2812
BsiHKAI	GWGCWC	2 cut(s)	1913, 2749
BsiSI	CCGG	3 cut(s)	1269, 2806, 3224
BslFI	GGGAC	5 cut(s)	123, 929, 1546, 2598, 3212
BslI	CCNNNNNNNGG	6 cut(s)	36, 953, 1251, 1645, 1691, 3067
BsmAI	GTCTC	4 cut(s)	110, 197, 2202, 3136
BsmBI	CGTCTC	1 cut(s)	197
BsmFI	GGGAC	5 cut(s)	123, 929, 1546, 2598, 3212
BsmI	GAATGC	5 cut(s)	196, 1745, 2496, 2907, 2975
BsnI	GGCC	4 cut(s)	1412, 2805, 3196, 3262
Bso31I	GGTCTC	1 cut(s)	110
Bsp1286I	GDGCHC	4 cut(s)	1502, 1913, 2749, 2815
Bsp1720I	GCTNAGC	1 cut(s)	1501
BspANI	GGCC	4 cut(s)	1412, 2805, 3196, 3262
BspCNI	CTCAG	7 cut(s)	222, 706, 939, 1157, 1514, 1940, 2269
BspFNI	CGCG	3 cut(s)	143, 833, 2237
BspHI	TCATGA	2 cut(s)	861, 2467
BspLI	GGNNCC	7 cut(s)	19, 165, 632, 2133, 2814, 2843, 3228
BspMAI	CTGCAG	1 cut(s)	2922
BspMI	ACCTGC	1 cut(s)	1165
BspOI	GCTAGC	2 cut(s)	1244, 1430
BspPI	GGATC	8 cut(s)	158, 171, 312, 595, 1038, 1720, 2126, 2139
BspQI	GCTCTTC	1 cut(s)	2058
BspT107I	GGYRCC	1 cut(s)	2812
BspTNI	GGTCTC	1 cut(s)	110
BsrBI	CCGCTC	1 cut(s)	2648
BsrDI	GCAATG	5 cut(s)	174, 344, 1362, 2019, 3164
BssECI	CCNNGG	4 cut(s)	141, 456, 602, 3118
BssSI	CACGAG	2 cut(s)	200, 1906
Bst2BI	CACGAG	2 cut(s)	200, 1906
Bst2UI	CCWGG	2 cut(s)	457, 2145
Bst6I	CTCTTC	4 cut(s)	816, 2058, 2683, 3068
BstBAI	YACGTR	2 cut(s)	14, 2408
BstC8I	GCNNGC	9 cut(s)	225, 748, 1138, 1242, 1428, 1940, 2112, 2400, 2940
BstDEI	CTNAG	8 cut(s)	209, 714, 926, 1144, 1501, 1640, 1927, 2277
BstDSI	CCRYGG	1 cut(s)	141
BstENI	CCTNNNNNAGG	1 cut(s)	1643
BstF5I	GGATG	6 cut(s)	315, 737, 1618, 1764, 2551, 2932
BstFNI	CGCG	3 cut(s)	143, 833, 2237
BstHHI	GCGC	3 cut(s)	196, 400, 1657
BstMAI	GTCTC	4 cut(s)	110, 197, 2202, 3136
BstMWI	GCNNNNNNNGC	5 cut(s)	143, 1399, 1460, 3160, 3268
BstNI	CCWGG	2 cut(s)	457, 2145
BstNSI	RCATGY	2 cut(s)	157, 2942
BstSCI	CCNGG	2 cut(s)	455, 2143
BstSFI	CTRYAG	3 cut(s)	1788, 2772, 2918
BstSLI	GKGCMC	2 cut(s)	1913, 2815
BstSNI	TACGTA	1 cut(s)	2408
BstUI	CGCG	3 cut(s)	143, 833, 2237
BstV1I	GCAGC	7 cut(s)	124, 353, 692, 1216, 1898, 2656, 3166
BstV2I	GAAGAC	1 cut(s)	18
BstX2I	RGATCY	6 cut(s)	163, 317, 1282, 1712, 2131, 2366
BstYI	RGATCY	6 cut(s)	163, 317, 1282, 1712, 2131, 2366
BsuI	GTATCC	2 cut(s)	1144, 2728
BsuRI	GGCC	4 cut(s)	1412, 2805, 3196, 3262
BtgI	CCRYGG	1 cut(s)	141
BtgZI	GCGATG	2 cut(s)	661, 2218
BtsCI	GGATG	6 cut(s)	315, 737, 1618, 1764, 2551, 2932
BtsI	GCAGTG	5 cut(s)	690, 1353, 1395, 1525, 2166
BveI	ACCTGC	1 cut(s)	1165
Cac8I	GCNNGC	9 cut(s)	225, 748, 1138, 1242, 1428, 1940, 2112, 2400, 2940
CaiI	CAGNNNCTG	3 cut(s)	1229, 1394, 2713
CciI	TCATGA	2 cut(s)	861, 2467
CfoI	GCGC	3 cut(s)	196, 400, 1657
Cfr13I	GGNCC	1 cut(s)	3226
Cfr42I	CCGCGG	1 cut(s)	144
CseI	GACGC	3 cut(s)	143, 214, 1432
Csp6I	GTAC	9 cut(s)	262, 920, 1083, 1298, 2192, 2705, 3092, 3250, 3274
CviQI	GTAC	9 cut(s)	262, 920, 1083, 1298, 2192, 2705, 3092, 3250, 3274
DdeI	CTNAG	8 cut(s)	209, 714, 926, 1144, 1501, 1640, 1927, 2277
DraI	TTTAAA	2 cut(s)	1306, 1545
EaeI	YGGCCR	2 cut(s)	1410, 2803
Eam1104I	CTCTTC	4 cut(s)	816, 2058, 2683, 3068
EarI	CTCTTC	4 cut(s)	816, 2058, 2683, 3068
EciI	GGCGGA	3 cut(s)	322, 631, 1584
Eco105I	TACGTA	1 cut(s)	2408
Eco24I	GRGCYC	1 cut(s)	1502
Eco31I	GGTCTC	1 cut(s)	110
Eco32I	GATATC	1 cut(s)	2533
Eco47I	GGWCC	1 cut(s)	3226
Eco57I	CTGAAG	7 cut(s)	797, 1416, 2196, 2529, 3042, 3080, 3238
Eco72I	CACGTG	1 cut(s)	14
EcoNI	CCTNNNNNAGG	1 cut(s)	1643
EcoRI	GAATTC	1 cut(s)	540
EcoRII	CCWGG	2 cut(s)	455, 2143
EcoRV	GATATC	1 cut(s)	2533
EcoT22I	ATGCAT	1 cut(s)	2977
EcoT38I	GRGCYC	1 cut(s)	1502
Esp3I	CGTCTC	1 cut(s)	197
FalI	AAGNNNNNCTT	2 cut(s)	2085, 2117
FaqI	GGGAC	5 cut(s)	123, 929, 1546, 2598, 3212
FauNDI	CATATG	3 cut(s)	1570, 2035, 3167
FbaI	TGATCA	3 cut(s)	672, 937, 2727
FblI	GTMKAC	2 cut(s)	1474, 1787
FokI	GGATG	6 cut(s)	322, 744, 1605, 1771, 2558, 2939
FriOI	GRGCYC	1 cut(s)	1502
FspBI	CTAG	8 cut(s)	434, 996, 1241, 1286, 1320, 1427, 1901, 2741
FspI	TGCGCA	1 cut(s)	1656
GlaI	GCGC	3 cut(s)	195, 399, 1656
GsaI	CCCAGC	1 cut(s)	2584
GsuI	CTGGAG	4 cut(s)	134, 646, 2226, 2442
HaeIII	GGCC	4 cut(s)	1412, 2805, 3196, 3262
HapII	CCGG	3 cut(s)	1269, 2806, 3224
HgaI	GACGC	3 cut(s)	143, 214, 1432
HhaI	GCGC	3 cut(s)	196, 400, 1657
Hin6I	GCGC	3 cut(s)	194, 398, 1655
HinP1I	GCGC	3 cut(s)	194, 398, 1655
HincII	GTYRAC	1 cut(s)	2722
HindII	GTYRAC	1 cut(s)	2722
HindIII	AAGCTT	1 cut(s)	642
HinfI	GANTC	9 cut(s)	658, 957, 992, 1107, 1784, 1856, 1925, 3081, 3235
HpaI	GTTAAC	1 cut(s)	2722
HpaII	CCGG	3 cut(s)	1269, 2806, 3224
HphI	GGTGA	8 cut(s)	428, 923, 947, 1019, 1169, 1351, 1363, 2429
Hpy166II	GTNNAC	6 cut(s)	264, 1024, 1475, 1788, 1911, 2722
Hpy8I	GTNNAC	6 cut(s)	264, 1024, 1475, 1788, 1911, 2722
Hpy99I	CGWCG	3 cut(s)	55, 137, 163
HpyAV	CCTTC	7 cut(s)	178, 529, 722, 1391, 1805, 2288, 2917
HpyCH4IV	ACGT	7 cut(s)	13, 50, 528, 1296, 1674, 2407, 2587
HpyF10VI	GCNNNNNNNGC	5 cut(s)	143, 1399, 1460, 3160, 3268
HpyF3I	CTNAG	8 cut(s)	209, 714, 926, 1144, 1501, 1640, 1927, 2277
HpySE526I	ACGT	7 cut(s)	13, 50, 528, 1296, 1674, 2407, 2587
HspAI	GCGC	3 cut(s)	194, 398, 1655
Ksp22I	TGATCA	3 cut(s)	672, 937, 2727
KspAI	GTTAAC	1 cut(s)	2722
KspI	CCGCGG	1 cut(s)	144
LguI	GCTCTTC	1 cut(s)	2058
LmnI	GCTCC	6 cut(s)	61, 743, 1343, 1883, 2461, 2744
Lsp1109I	GCAGC	7 cut(s)	124, 353, 692, 1216, 1898, 2656, 3166
LweI	GCATC	5 cut(s)	1149, 1627, 2146, 2260, 2443
MaeI	CTAG	8 cut(s)	434, 996, 1241, 1286, 1320, 1427, 1901, 2741
MaeII	ACGT	7 cut(s)	13, 50, 528, 1296, 1674, 2407, 2587
MbiI	CCGCTC	1 cut(s)	2648
MfeI	CAATTG	1 cut(s)	851
MflI	RGATCY	6 cut(s)	163, 317, 1282, 1712, 2131, 2366
MhlI	GDGCHC	4 cut(s)	1502, 1913, 2749, 2815
MlyI	GAGTC	5 cut(s)	652, 1793, 1850, 1919, 3229
MmeI	TCCRAC	2 cut(s)	39, 1084
Mph1103I	ATGCAT	1 cut(s)	2977
MroXI	GAANNNNTTC	1 cut(s)	1355
MslI	CAYNNNNRTG	4 cut(s)	1376, 2948, 3044, 3110
MspA1I	CMGCKG	5 cut(s)	143, 788, 1624, 1889, 3271
MspI	CCGG	3 cut(s)	1269, 2806, 3224
MspR9I	CCNGG	2 cut(s)	457, 2145
MunI	CAATTG	1 cut(s)	851
Mva1269I	GAATGC	5 cut(s)	196, 1745, 2496, 2907, 2975
MvaI	CCWGG	2 cut(s)	457, 2145
MvnI	CGCG	3 cut(s)	143, 833, 2237
MwoI	GCNNNNNNNGC	5 cut(s)	143, 1399, 1460, 3160, 3268
NdeI	CATATG	3 cut(s)	1570, 2035, 3167
NheI	GCTAGC	2 cut(s)	1240, 1426
NlaIV	GGNNCC	7 cut(s)	19, 165, 632, 2133, 2814, 2843, 3228
NmuCI	GTSAC	5 cut(s)	9, 781, 1369, 2200, 3046
NsbI	TGCGCA	1 cut(s)	1656
NsiI	ATGCAT	1 cut(s)	2977
NspI	RCATGY	2 cut(s)	157, 2942
OliI	CACNNNNGTG	1 cut(s)	1376
PaeI	GCATGC	1 cut(s)	2942
PagI	TCATGA	2 cut(s)	861, 2467
PaqCI	CACCTGC	1 cut(s)	1165
PciSI	GCTCTTC	1 cut(s)	2058
PcsI	WCGNNNNNNNCGW	2 cut(s)	29, 2584
PctI	GAATGC	5 cut(s)	196, 1745, 2496, 2907, 2975
PdmI	GAANNNNTTC	1 cut(s)	1355
PfeI	GAWTC	4 cut(s)	957, 992, 1107, 3081
PflMI	CCANNNNNTGG	1 cut(s)	1691
PleI	GAGTC	5 cut(s)	652, 1792, 1850, 1919, 3229
PmaCI	CACGTG	1 cut(s)	14
PmlI	CACGTG	1 cut(s)	14
PpsI	GAGTC	5 cut(s)	652, 1792, 1850, 1919, 3229
Ppu21I	YACGTR	2 cut(s)	14, 2408
PshBI	ATTAAT	1 cut(s)	2658
PsiI	TTATAA	2 cut(s)	665, 2822
Psp6I	CCWGG	2 cut(s)	455, 2143
PspCI	CACGTG	1 cut(s)	14
PspFI	CCCAGC	1 cut(s)	2580
PspGI	CCWGG	2 cut(s)	455, 2143
PspN4I	GGNNCC	7 cut(s)	19, 165, 632, 2133, 2814, 2843, 3228
PspPI	GGNCC	1 cut(s)	3226
PsrI	GAACNNNNNNTAC	2 cut(s)	2559, 2591
PstI	CTGCAG	1 cut(s)	2922
PstNI	CAGNNNCTG	3 cut(s)	1229, 1394, 2713
PsuI	RGATCY	6 cut(s)	163, 317, 1282, 1712, 2131, 2366
PvuII	CAGCTG	1 cut(s)	788
RsaI	GTAC	9 cut(s)	263, 921, 1084, 1299, 2193, 2706, 3093, 3251, 3275
RsaNI	GTAC	9 cut(s)	262, 920, 1083, 1298, 2192, 2705, 3092, 3250, 3274
RseI	CAYNNNNRTG	4 cut(s)	1376, 2948, 3044, 3110
SacII	CCGCGG	1 cut(s)	144
SapI	GCTCTTC	1 cut(s)	2058
Sau96I	GGNCC	1 cut(s)	3226
ScaI	AGTACT	1 cut(s)	3251
SchI	GAGTC	5 cut(s)	652, 1793, 1850, 1919, 3229
ScrFI	CCNGG	2 cut(s)	457, 2145
SduI	GDGCHC	4 cut(s)	1502, 1913, 2749, 2815
SfaNI	GCATC	5 cut(s)	1149, 1627, 2146, 2260, 2443
SfcI	CTRYAG	3 cut(s)	1788, 2772, 2918
Sfr303I	CCGCGG	1 cut(s)	144
SgrBI	CCGCGG	1 cut(s)	144
SinI	GGWCC	1 cut(s)	3226
SmiMI	CAYNNNNRTG	4 cut(s)	1376, 2948, 3044, 3110
SnaBI	TACGTA	1 cut(s)	2408
SphI	GCATGC	1 cut(s)	2942
SspI	AATATT	4 cut(s)	469, 894, 1948, 2605
SspMI	CTAG	8 cut(s)	434, 996, 1241, 1286, 1320, 1427, 1901, 2741
StyD4I	CCNGG	2 cut(s)	455, 2143
TaiI	ACGT	7 cut(s)	16, 53, 531, 1299, 1677, 2410, 2590
TaqI	TCGA	7 cut(s)	126, 171, 524, 1701, 2265, 2292, 3013
TaqII	GACCGA	1 cut(s)	134
TatI	WGTACW	2 cut(s)	2704, 3249
TauI	GCSGC	4 cut(s)	143, 146, 230, 2648
TfiI	GAWTC	4 cut(s)	957, 992, 1107, 3081
TseFI	GTSAC	5 cut(s)	9, 781, 1369, 2200, 3046
TseI	GCWGC	8 cut(s)	5, 137, 341, 680, 1229, 1886, 2669, 3154
Tsp45I	GTSAC	5 cut(s)	9, 781, 1369, 2200, 3046
TspGWI	ACGGA	7 cut(s)	176, 438, 756, 1736, 2297, 2682, 3228
Van91I	CCANNNNNTGG	1 cut(s)	1691
VneI	GTGCAC	1 cut(s)	1909
VpaK11BI	GGWCC	1 cut(s)	3226
VspI	ATTAAT	1 cut(s)	2658
XagI	CCTNNNNNAGG	1 cut(s)	1643
XapI	RAATTY	6 cut(s)	540, 1353, 2612, 2991, 3125, 3280
XbaI	TCTAGA	1 cut(s)	1285
XceI	RCATGY	2 cut(s)	157, 2942
XmiI	GTMKAC	2 cut(s)	1474, 1787
XmnI	GAANNNNTTC	1 cut(s)	1355
XspI	CTAG	8 cut(s)	434, 996, 1241, 1286, 1320, 1427, 1901, 2741
ZrmI	AGTACT	1 cut(s)	3251
Zsp2I	ATGCAT	1 cut(s)	2977

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.