Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0008228

Physical Location & Seq

Genomic Coordinates Reverse (-)

18824598 .. 18839250

Gene Structure 14653 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00097600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3372 bp

ATGGCGTCCGGTGATTCCGACTCTTCCAAGAACGGGTTGCTTCCGACGTCGAGGTCCGGCCGCCACCACCTCGACATGGAGTCCGGCGCCAAGGTCCGCCTCCCCGCCGAGTTCGTCGAAGGCAAGGCGTCCAAGAGCGACGATCACCACGACGACGATCCCTTCGACATTGCCCACACCAAGAACGCGCCTCCCGCCACGCTACGCCGATGGAGGAGGTCTGATTCTTTGCAGCAAGCCGCGCTAGTGTTAAATGCTTCACGTCGATTTCGGTACACGTTGGACTTAAAAAAGGAAGAAGAGAAAGACATTCAATTGATGATTACTTGTGATATTAGGCCGCATTGCTTTTCAAATTGGCTGGAGAACGGGAAACTGGTGCCATTCCAGTTCATTATTCTCATAATTCACTTATTTCCGGATTATATAGGGTTAGGCATAAAAGTAACACCTCCAACTCCAAGCGGTGATTATGGAATTGGACTTGAACCACTTGTTTCACTAACTAGGGAGCATAATACTTCATCTCTACAGCAATATGGAGGGGTTAAAGGTATATCATCTTTGATAAAAACAAATTTGGAGAAGGGAATTGATGGGGATGAAGCAGATTTAGTATATCGCAGAAATATGTTTGGATCAAATACATATCCTCAGAAAAAAGGGCGGAGTTTCTTGAGGTTCCTTTGGGAAGCATGGCAAGATTTAACTCTTATAATCTTGATAATAGCTGCAGCGGTGTCATTGGCACTTGGAATTAAAACAGAGGGTTTGGCTCACGGATGGTATGATGGAGGAAGCATTGCATTTGCTGTTTTTCTTGTTATCATTGTTACAGCTGTTAGTGATTATCGGCAATCTCTTCAGTTTCAAAATCTAAATGAGGAAAAGCAAAATATACAATTAGAGGTCATGAGAGGCGGTAGAACAGTAAAGATTTCAATATTTGATATTGTGGTTGGTGATGTTGTACCTCTCAGAATAGGTGATCAGGTCCCGGCTGATGGAATCTTAATCACCGGTCATTCTCTTGCGATTGATGAATCTAGTATGACCGGCGAGAGCAAAATTGTTCACAAGGATCAGAAAGCACCATTCTTAATGTCTGGCTGCAAAATAGCTGATGGAGTGGGTACCATGCTGGTAACGAGTGTTGGAATCAATACAGAATGGGGATTGTTGATGGCAAGTATCTCTGAGGATAATGGTGAAGAAACTCCTTTACAGGTGCGCTTAAATGGACTTGCGACTTTTATAGGCATAGTTGGGCTTACAGTAGCTGCTTCGGTGCTTCTTGTTCTTTGGTTCAGATACTTTACTGGACATTCAAAAAATACAGATGGAAGTGTGGAATTTGAAGCGGGAAAGACTAGCCTTAGTAAGGCTGTAGATGGAGCTGTAAAAATTTTCACCATTGCTGTCACCATTGTAGTTGTTGCAGTGCCTGAAGGGCTACCTTTGGCTGTTACCTTGACCATATGTGTGCAGGTGCGTAGACTGTCGGCTTGTGAAACTATGGGCTCAGCAACAACAATTTGCAGTGATAAAACTGGCACATTGACTTTAAATCAGATGACTGTGGTTGAGGCATATGTTGGGAGAAACAAGATACATCCACCAGATGATTCCTCTCAGTTGCATCAACAGGTTTCCACTCTGCTAAGTGAGGGCATTGCGCAGAATACCAGTGGAAATGTGTTTGAGCCAGAGGGTGGTGAAGTAGAGATCTCTGGATCTCCCACAGAAAAGGCTATACTTTCGTGGGCACTGAAGGTTCCATTTCAATCCGCTAAAGTTTTTACTTTCAGTTTCCTATATTTGAATATTTACTCAGCGATCAAATCTGCATTTCAGTTGGGAATGAAGTTTAATGCTATCAGATCCGAGTCCATGATTCTTCATGTGTTCCCTTTCAACTCAGAGAAAAAGCGAGGAGGTGTTGCACTGAAAGGGTCTGACTCCAAAGTTCATATACATTGGAAGGGCGCTGCGGAGATAGTTTTAGATTCATGTACAGAGTATCTCGATTCAAATGGGTGTTTGCAGGACATTAATCAAGATAAGGATTTTTTCAAGGCAGCTATCGATGGCATGGCAGCAAGCAGTTTGCGATGCGTTGCCATTGCATACAGACGATATGAATTGGACAAAGTTCCTACTGAGGAAGAGAATTTAAGTCAATGGGCTTTACCTGAAGATAATCTAGTGTTGCTTGCTATCGTTGGTATAAAGGATCCTTGTCGCCTTGGGGTCAAAGATGCAGTGAGATTATGCACAGAGGCTGGTGTTAAGGTACGCATGGTCACTGGAGACAACCTTCAGACAGCAAAAGCAATCGCGTTAGAGTGTGGAATTCTTTGTTCTGTTGAGGATGCTACGGAGCCTAATATCATTGAAGGAAAAACATTCCGTGAATTACCAGAAAAAGAAAGAGAAGAAGTTGCCCAGAAAATAACGTGTGAGTTGTACGTCGGCATTACGGAAGGGGGAGATGTTGTTGCAGTAACAGGAGATGGCACTAATGATGCTCCTGCCCTTCACGAGGCAGATATTGGTCTATCTATGGGCATTCAAGGAACTGAAGTTGCAAAAGAAAGTTCGGATATCATTATCTTGGATGATAATTTTGCTTCTGTTGTAAAGGTTGTTCGCTGGGGACGTTCTGTGTATGCAAATATTCAGAAATTTATCCAATTCCAGCTTACGGTTAATGTTGCTGCTCTTACAATTAATGTTGTTGCAGCAATATCCTCTGGTCATCTTCTGTGGGTTAACTTGATTATGGATACTCTAGGAGCACTTGCATTGGCAACTGAACCACCTACAGACGACCTTATGCATAGAGCACCAGTTGGCAGAAGGGCACCTCTTATAACCAACATCATGTGGAGGAACCTACTAATTCAGGCTTTGTATCAAGTTTGTGTGCTCCTTGTCCTCAACTTTTTGGGTAATAGCATTCTTGGTCTGAAAGAGGAGAAGACACAAGCCGCTGGTGTGAAGAATACAATCATATTCAATGCTTTTGTCTTCTGCCAAATTTTCAATGAGTTTAATGCTCGAAAGCCTGAAGAGCTGAATGTTTTCAGTGGAGTGACTAAGAACTACCTTTTTATGGGAATAATAGGAGTCACATTTGTACTTCAGATACTCATCATTCAGTTTCTTGGAACGTTCGCAAAAACAGTGGTACTTGATTGGAAGCAATGGCTTATTTGCTTTGGTATTGCCATCATCAGCTGGCCTCTTGCTATTGTCGGCAAACTAATTCCGGTCCCTAAGACTCCATTCTCCCAGTACTGCAGGCGCGCATTTCAGCCATGCAGACAGTCATTTCAGCGAAGCAGAACTTCTCACAATACCCAAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007275 GO:0007338 GO:0008150 GO:0008324 GO:0009555 GO:0009566 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019953 GO:0022414 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044703 GO:0046873 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0051704 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1123

Amino Acids

123.06

Weight (kDa)

5.92

Isoelectric Point (pI)

40.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	52 - 101	7.9e-12	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	180 - 248	3.9e-13	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	301 - 397	4.5e-19	P-type ATPase actuator domain
Hydrolase	PF00702	511 - 850	2.6e-10	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	550 - 749	2e-27	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	915 - 1090	4.1e-43	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008228)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21180
fragaria_vesca	FvH4_6g37190 FvH4_6g37190 FvH4_6g37190 FvH4_6g37190
malus_domestica	MD09G1160300.v1.1 MD17G1146100.v1.1
prunus_persica	Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1
pyrus_communis	pycom09g07890 pycom17g13890
rosa_chinensis	RchiOBHm_Chr2g0149841
rosa_laevigata	RLG00000020450
rosa_multiflora	Rmu_ssc0000308.1_g000029
rosa_roxburghii	Rroxscaffold_2G00097600
rosa_rugosa	Rorug02G0417000
rosa_samantha	Rh2BG487700 Rh2CG461600 Rh2DG496500
rosa_wichuraiana	Rw2G038820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	716, 2873
AarI	CACCTGC	1 cut(s)	1477
AasI	GACNNNNNNGTC	1 cut(s)	52
AatII	GACGTC	1 cut(s)	50
Acc16I	TGCGCA	1 cut(s)	1677
Acc36I	ACCTGC	1 cut(s)	1477
Acc65I	GGTACC	1 cut(s)	1133
AccB1I	GGYRCC	4 cut(s)	86, 379, 1133, 2863
AccB7I	CCANNNNNTGG	1 cut(s)	1712
AccI	GTMKAC	1 cut(s)	1495
AccII	CGCG	4 cut(s)	188, 242, 2339, 3309
AccIII	TCCGGA	1 cut(s)	418
AclI	AACGTT	1 cut(s)	3173
AclWI	GGATC	7 cut(s)	152, 646, 1089, 1741, 1875, 2228, 2241
AcoI	YGGCCR	1 cut(s)	58
AcsI	RAATTY	7 cut(s)	577, 1352, 1404, 2170, 2352, 2684, 3039
AcuI	CTGAAG	8 cut(s)	848, 1467, 1790, 2214, 2303, 2601, 3090, 3128
AcyI	GRCGYC	4 cut(s)	5, 47, 87, 128
AfaI	GTAC	9 cut(s)	275, 972, 1135, 2014, 2295, 2468, 3141, 3192, 3299
AfiI	CCNNNNNNNGG	8 cut(s)	33, 76, 1004, 1381, 1712, 2542, 2704, 3115
AflIII	ACRYGT	2 cut(s)	276, 2456
AgeI	ACCGGT	1 cut(s)	1019
AhdI	GACNNNNNGTC	1 cut(s)	79
AjiI	CACGTC	1 cut(s)	263
Alw21I	GWGCWC	3 cut(s)	2800, 2848, 2931
Alw26I	GTCTC	1 cut(s)	2304
AlwI	GGATC	7 cut(s)	152, 646, 1089, 1741, 1875, 2228, 2241
AlwNI	CAGNNNCTG	3 cut(s)	1280, 1445, 2282
Aor13HI	TCCGGA	1 cut(s)	418
AoxI	GGCC	3 cut(s)	58, 338, 3242
ApoI	RAATTY	7 cut(s)	577, 1352, 1404, 2170, 2352, 2684, 3039
ArsI	GACNNNNNNTTYG	2 cut(s)	146, 178
AseI	ATTAAT	2 cut(s)	2052, 2730
AsiGI	ACCGGT	1 cut(s)	1019
Asp700I	GAANNNNTTC	1 cut(s)	3083
Asp718I	GGTACC	1 cut(s)	1133
AspLEI	GCGC	8 cut(s)	89, 190, 244, 1233, 1678, 1988, 3309, 3311
AspS9I	GGNCC	4 cut(s)	54, 94, 994, 3274
AsuC2I	CCSGG	1 cut(s)	998
AvaII	GGWCC	4 cut(s)	54, 94, 994, 3274
BaeGI	GKGCMC	2 cut(s)	1768, 2866
BamHI	GGATCC	1 cut(s)	2233
BanI	GGYRCC	4 cut(s)	86, 379, 1133, 2863
BanII	GRGCYC	1 cut(s)	1523
BauI	CACGAG	1 cut(s)	2540
BbsI	GAAGAC	2 cut(s)	2987, 3022
Bbv12I	GWGCWC	3 cut(s)	2800, 2848, 2931
BciVI	GTATCC	1 cut(s)	2779
BclI	TGATCA	1 cut(s)	988
BcnI	CCSGG	1 cut(s)	998
BcoDI	GTCTC	1 cut(s)	2304
BfaI	CTAG	6 cut(s)	245, 507, 1047, 1371, 2204, 2792
BfmI	CTRYAG	5 cut(s)	530, 732, 1386, 2823, 3301
BfoI	RGCGCY	2 cut(s)	90, 1989
BfuAI	ACCTGC	1 cut(s)	1477
BfuI	GTATCC	1 cut(s)	2779
BglI	GCCNNNNNGGC	1 cut(s)	1450
BglII	AGATCT	1 cut(s)	1725
BlpI	GCTNAGC	1 cut(s)	1522
BmcAI	AGTACT	1 cut(s)	3299
Bme1390I	CCNGG	1 cut(s)	998
Bme18I	GGWCC	4 cut(s)	54, 94, 994, 3274
BmeRI	GACNNNNNGTC	1 cut(s)	79
BmgBI	CACGTC	1 cut(s)	263
BmgT120I	GGNCC	4 cut(s)	54, 94, 994, 3274
BmrFI	CCNGG	1 cut(s)	998
BmrI	ACTGGG	1 cut(s)	3289
BmsI	GCATC	5 cut(s)	1648, 2102, 2248, 2362, 2515
BmuI	ACTGGG	1 cut(s)	3289
BpiI	GAAGAC	2 cut(s)	2987, 3022
BpmI	CTGGAG	2 cut(s)	383, 2328
Bpu1102I	GCTNAGC	1 cut(s)	1522
BpuEI	CTTGAG	1 cut(s)	697
BpuMI	CCSGG	1 cut(s)	998
Bsa29I	ATCGAT	1 cut(s)	2085
BsaBI	GATNNNNATC	1 cut(s)	3152
BsaHI	GRCGYC	4 cut(s)	5, 47, 87, 128
BsaJI	CCNNGG	2 cut(s)	90, 2245
BsaWI	WCCGGW	4 cut(s)	8, 418, 1019, 3271
BsaXI	ACNNNNNCTCC	2 cut(s)	2481, 2511
Bsc4I	CCNNNNNNNGG	8 cut(s)	33, 76, 1004, 1381, 1712, 2542, 2704, 3115
Bse118I	RCCGGY	2 cut(s)	1019, 1055
Bse1I	ACTGG	8 cut(s)	381, 388, 1324, 1555, 1686, 2311, 2849, 3295
Bse3DI	GCAATG	7 cut(s)	168, 343, 801, 1413, 1671, 2121, 3212
Bse8I	GATNNNNATC	1 cut(s)	3152
BseAI	TCCGGA	1 cut(s)	418
BseCI	ATCGAT	1 cut(s)	2085
BseDI	CCNNGG	2 cut(s)	90, 2245
BseGI	GGATG	5 cut(s)	607, 788, 1612, 2377, 2623
BseJI	GATNNNNATC	1 cut(s)	3152
BseLI	CCNNNNNNNGG	8 cut(s)	33, 76, 1004, 1381, 1712, 2542, 2704, 3115
BseMI	GCAATG	7 cut(s)	168, 343, 801, 1413, 1671, 2121, 3212
BseMII	CTCAG	8 cut(s)	668, 991, 1188, 1536, 1646, 1845, 1932, 2151
BseNI	ACTGG	8 cut(s)	381, 388, 1324, 1555, 1686, 2311, 2849, 3295
BsePI	GCGCGC	1 cut(s)	3307
BseRI	GAGGAG	3 cut(s)	229, 1947, 2990
BseSI	GKGCMC	2 cut(s)	1768, 2866
BseX3I	CGGCCG	1 cut(s)	58
BseYI	CCCAGC	1 cut(s)	2652
BsgI	GTGCAG	1 cut(s)	1505
Bsh1236I	CGCG	4 cut(s)	188, 242, 2339, 3309
Bsh1285I	CGRYCG	1 cut(s)	61
BshFI	GGCC	3 cut(s)	60, 340, 3244
BshNI	GGYRCC	4 cut(s)	86, 379, 1133, 2863
BshTI	ACCGGT	1 cut(s)	1019
BshVI	ATCGAT	1 cut(s)	2085
BsiEI	CGRYCG	1 cut(s)	61
BsiHKAI	GWGCWC	3 cut(s)	2800, 2848, 2931
BsiSI	CCGG	8 cut(s)	9, 57, 84, 419, 998, 1020, 1056, 3272
BslFI	GGGAC	3 cut(s)	980, 2670, 3260
BslI	CCNNNNNNNGG	8 cut(s)	33, 76, 1004, 1381, 1712, 2542, 2704, 3115
BsmAI	GTCTC	1 cut(s)	2304
BsmFI	GGGAC	3 cut(s)	980, 2670, 3260
BsmI	GAATGC	2 cut(s)	2568, 2958
BsnI	GGCC	3 cut(s)	60, 340, 3244
Bsp1286I	GDGCHC	6 cut(s)	1523, 1768, 2800, 2848, 2866, 2931
Bsp13I	TCCGGA	1 cut(s)	418
Bsp1407I	TGTACA	1 cut(s)	2012
Bsp1720I	GCTNAGC	1 cut(s)	1522
BspANI	GGCC	3 cut(s)	60, 340, 3244
BspCNI	CTCAG	8 cut(s)	667, 990, 1189, 1535, 1645, 1844, 1931, 2152
BspDI	ATCGAT	1 cut(s)	2085
BspEI	TCCGGA	1 cut(s)	418
BspFNI	CGCG	4 cut(s)	188, 242, 2339, 3309
BspHI	TCATGA	1 cut(s)	912
BspMAI	CTGCAG	2 cut(s)	736, 3305
BspMI	ACCTGC	1 cut(s)	1477
BspPI	GGATC	7 cut(s)	152, 646, 1089, 1741, 1875, 2228, 2241
BspQI	GCTCTTC	1 cut(s)	3066
BspT107I	GGYRCC	4 cut(s)	86, 379, 1133, 2863
BsrDI	GCAATG	7 cut(s)	168, 343, 801, 1413, 1671, 2121, 3212
BsrFI	RCCGGY	2 cut(s)	1019, 1055
BsrGI	TGTACA	1 cut(s)	2012
BsrI	ACTGG	8 cut(s)	381, 388, 1324, 1555, 1686, 2311, 2849, 3295
BssAI	RCCGGY	2 cut(s)	1019, 1055
BssECI	CCNNGG	2 cut(s)	90, 2245
BssHII	GCGCGC	1 cut(s)	3307
BssNI	GRCGYC	4 cut(s)	5, 47, 87, 128
BssSI	CACGAG	1 cut(s)	2540
BssT1I	CCWWGG	2 cut(s)	90, 2245
Bst2BI	CACGAG	1 cut(s)	2540
Bst4CI	ACNGT	7 cut(s)	931, 1276, 1500, 1579, 2707, 3187, 3330
Bst6I	CTCTTC	5 cut(s)	28, 294, 867, 2160, 3066
BstACI	GRCGYC	4 cut(s)	5, 47, 87, 128
BstAUI	TGTACA	1 cut(s)	2012
BstC8I	GCNNGC	6 cut(s)	237, 2101, 2214, 3242, 3305, 3309
BstENI	CCTNNNNNAGG	2 cut(s)	1379, 2540
BstF5I	GGATG	5 cut(s)	607, 788, 1612, 2377, 2623
BstFNI	CGCG	4 cut(s)	188, 242, 2339, 3309
BstH2I	RGCGCY	2 cut(s)	90, 1989
BstHHI	GCGC	8 cut(s)	89, 190, 244, 1233, 1678, 1988, 3309, 3311
BstMAI	GTCTC	1 cut(s)	2304
BstMCI	CGRYCG	1 cut(s)	61
BstMWI	GCNNNNNNNGC	4 cut(s)	194, 241, 1450, 3073
BstSCI	CCNGG	1 cut(s)	996
BstSFI	CTRYAG	5 cut(s)	530, 732, 1386, 2823, 3301
BstSLI	GKGCMC	2 cut(s)	1768, 2866
BstUI	CGCG	4 cut(s)	188, 242, 2339, 3309
BstV2I	GAAGAC	2 cut(s)	2987, 3022
BstX2I	RGATCY	4 cut(s)	1725, 1733, 1880, 2233
BstYI	RGATCY	4 cut(s)	1725, 1733, 1880, 2233
BstZI	CGGCCG	1 cut(s)	58
Bsu15I	ATCGAT	1 cut(s)	2085
BsuI	GTATCC	1 cut(s)	2779
BsuRI	GGCC	3 cut(s)	60, 340, 3244
BsuTUI	ATCGAT	1 cut(s)	2085
BtgZI	GCGATG	1 cut(s)	2125
BtrI	CACGTC	1 cut(s)	263
BtsCI	GGATG	5 cut(s)	607, 788, 1612, 2377, 2623
BtsI	GCAGTG	3 cut(s)	1446, 1546, 2268
BtsIMutI	CAGTG	9 cut(s)	1446, 1546, 1693, 1766, 1943, 2268, 2304, 3094, 3192
BveI	ACCTGC	1 cut(s)	1477
Cac8I	GCNNGC	6 cut(s)	237, 2101, 2214, 3242, 3305, 3309
CaiI	CAGNNNCTG	3 cut(s)	1280, 1445, 2282
CciI	TCATGA	1 cut(s)	912
CfoI	GCGC	8 cut(s)	89, 190, 244, 1233, 1678, 1988, 3309, 3311
Cfr10I	RCCGGY	2 cut(s)	1019, 1055
Cfr13I	GGNCC	4 cut(s)	54, 94, 994, 3274
ClaI	ATCGAT	1 cut(s)	2085
CseI	GACGC	1 cut(s)	117
Csp6I	GTAC	9 cut(s)	274, 971, 1134, 2013, 2294, 2467, 3140, 3191, 3298
CspAI	ACCGGT	1 cut(s)	1019
CspCI	CAANNNNNGTGG	2 cut(s)	3168, 3203
CviQI	GTAC	9 cut(s)	274, 971, 1134, 2013, 2294, 2467, 3140, 3191, 3298
DinI	GGCGCC	1 cut(s)	88
DraI	TTTAAA	1 cut(s)	1566
DrdI	GACNNNNNNGTC	1 cut(s)	52
DriI	GACNNNNNGTC	1 cut(s)	79
DseDI	GACNNNNNNGTC	1 cut(s)	52
EaeI	YGGCCR	1 cut(s)	58
EagI	CGGCCG	1 cut(s)	58
Eam1104I	CTCTTC	5 cut(s)	28, 294, 867, 2160, 3066
Eam1105I	GACNNNNNGTC	1 cut(s)	79
EarI	CTCTTC	5 cut(s)	28, 294, 867, 2160, 3066
EciI	GGCGGA	2 cut(s)	86, 682
EclXI	CGGCCG	1 cut(s)	58
Eco130I	CCWWGG	2 cut(s)	90, 2245
Eco24I	GRGCYC	1 cut(s)	1523
Eco32I	GATATC	1 cut(s)	2605
Eco47I	GGWCC	4 cut(s)	54, 94, 994, 3274
Eco52I	CGGCCG	1 cut(s)	58
Eco57I	CTGAAG	8 cut(s)	848, 1467, 1790, 2214, 2303, 2601, 3090, 3128
EcoNI	CCTNNNNNAGG	2 cut(s)	1379, 2540
EcoO109I	RGGNCCY	1 cut(s)	994
EcoRI	GAATTC	1 cut(s)	2352
EcoRV	GATATC	1 cut(s)	2605
EcoT14I	CCWWGG	2 cut(s)	90, 2245
EcoT22I	ATGCAT	1 cut(s)	2841
EcoT38I	GRGCYC	1 cut(s)	1523
EgeI	GGCGCC	1 cut(s)	88
EheI	GGCGCC	1 cut(s)	88
ErhI	CCWWGG	2 cut(s)	90, 2245
FalI	AAGNNNNNCTT	2 cut(s)	3334, 3366
FaqI	GGGAC	3 cut(s)	980, 2670, 3260
FauI	CCCGC	3 cut(s)	112, 202, 1354
FauNDI	CATATG	2 cut(s)	1478, 1591
FbaI	TGATCA	1 cut(s)	988
FblI	GTMKAC	1 cut(s)	1495
FokI	GGATG	5 cut(s)	614, 795, 1599, 2384, 2630
FriOI	GRGCYC	1 cut(s)	1523
FspBI	CTAG	6 cut(s)	245, 507, 1047, 1371, 2204, 2792
FspI	TGCGCA	1 cut(s)	1677
GlaI	GCGC	8 cut(s)	88, 189, 243, 1232, 1677, 1987, 3308, 3310
GsaI	CCCAGC	1 cut(s)	2656
GsuI	CTGGAG	2 cut(s)	383, 2328
HaeII	RGCGCY	2 cut(s)	90, 1989
HaeIII	GGCC	3 cut(s)	60, 340, 3244
HapII	CCGG	8 cut(s)	9, 57, 84, 419, 998, 1020, 1056, 3272
HgaI	GACGC	1 cut(s)	117
HhaI	GCGC	8 cut(s)	89, 190, 244, 1233, 1678, 1988, 3309, 3311
Hin1I	GRCGYC	4 cut(s)	5, 47, 87, 128
Hin6I	GCGC	8 cut(s)	87, 188, 242, 1231, 1676, 1986, 3307, 3309
HinP1I	GCGC	8 cut(s)	87, 188, 242, 1231, 1676, 1986, 3307, 3309
HincII	GTYRAC	1 cut(s)	2773
HindII	GTYRAC	1 cut(s)	2773
HpaI	GTTAAC	1 cut(s)	2773
HpaII	CCGG	8 cut(s)	9, 57, 84, 419, 998, 1020, 1056, 3272
Hpy166II	GTNNAC	4 cut(s)	276, 1075, 1496, 2773
Hpy188III	TCNNGA	8 cut(s)	419, 676, 721, 913, 1731, 2024, 2057, 2540
Hpy8I	GTNNAC	4 cut(s)	276, 1075, 1496, 2773
Hpy99I	CGWCG	8 cut(s)	49, 52, 119, 143, 155, 158, 267, 2474
HpyCH4III	ACNGT	7 cut(s)	931, 1276, 1500, 1579, 2707, 3187, 3330
HpyCH4IV	ACGT	7 cut(s)	47, 262, 278, 2456, 2469, 2659, 3173
HpyF10VI	GCNNNNNNNGC	4 cut(s)	194, 241, 1450, 3073
HpySE526I	ACGT	7 cut(s)	47, 262, 278, 2456, 2469, 2659, 3173
Hsp92I	GRCGYC	4 cut(s)	5, 47, 87, 128
HspAI	GCGC	8 cut(s)	87, 188, 242, 1231, 1676, 1986, 3307, 3309
KasI	GGCGCC	1 cut(s)	86
Kpn2I	TCCGGA	1 cut(s)	418
KpnI	GGTACC	1 cut(s)	1137
Ksp22I	TGATCA	1 cut(s)	988
KspAI	GTTAAC	1 cut(s)	2773
LguI	GCTCTTC	1 cut(s)	3066
LmnI	GCTCC	6 cut(s)	511, 1394, 2380, 2533, 2795, 2934
LweI	GCATC	5 cut(s)	1648, 2102, 2248, 2362, 2515
MaeI	CTAG	6 cut(s)	245, 507, 1047, 1371, 2204, 2792
MaeII	ACGT	7 cut(s)	47, 262, 278, 2456, 2469, 2659, 3173
MaeIII	GTNAC	9 cut(s)	445, 832, 1144, 1420, 1465, 2302, 2503, 3094, 3130
MfeI	CAATTG	1 cut(s)	314
MflI	RGATCY	4 cut(s)	1725, 1733, 1880, 2233
MhlI	GDGCHC	6 cut(s)	1523, 1768, 2800, 2848, 2866, 2931
Mly113I	GGCGCC	1 cut(s)	87
MlyI	GAGTC	6 cut(s)	14, 89, 1895, 1952, 3138, 3277
MmeI	TCCRAC	5 cut(s)	42, 68, 261, 479, 1135
Mph1103I	ATGCAT	1 cut(s)	2841
MroI	TCCGGA	1 cut(s)	418
MroXI	GAANNNNTTC	1 cut(s)	3083
MslI	CAYNNNNRTG	1 cut(s)	1481
MspA1I	CMGCKG	4 cut(s)	737, 839, 2993, 3240
MspI	CCGG	8 cut(s)	9, 57, 84, 419, 998, 1020, 1056, 3272
MspR9I	CCNGG	1 cut(s)	998
MunI	CAATTG	1 cut(s)	314
Mva1269I	GAATGC	2 cut(s)	2568, 2958
MvnI	CGCG	4 cut(s)	188, 242, 2339, 3309
MwoI	GCNNNNNNNGC	4 cut(s)	194, 241, 1450, 3073
NarI	GGCGCC	1 cut(s)	87
NciI	CCSGG	1 cut(s)	998
NdeI	CATATG	2 cut(s)	1478, 1591
NmeAIII	GCCGAG	1 cut(s)	133
NmuCI	GTSAC	4 cut(s)	1420, 2302, 3094, 3130
NsbI	TGCGCA	1 cut(s)	1677
NsiI	ATGCAT	1 cut(s)	2841
PagI	TCATGA	1 cut(s)	912
PaqCI	CACCTGC	1 cut(s)	1477
PauI	GCGCGC	1 cut(s)	3307
PciSI	GCTCTTC	1 cut(s)	3066
PcsI	WCGNNNNNNNCGW	3 cut(s)	147, 162, 2656
PctI	GAATGC	2 cut(s)	2568, 2958
PdmI	GAANNNNTTC	1 cut(s)	3083
PfeI	GAWTC	9 cut(s)	14, 224, 1008, 1043, 1158, 1625, 1894, 2006, 2027
PflMI	CCANNNNNTGG	1 cut(s)	1712
PinAI	ACCGGT	1 cut(s)	1019
PleI	GAGTC	6 cut(s)	14, 88, 1894, 1952, 3137, 3277
PluTI	GGCGCC	1 cut(s)	90
PpsI	GAGTC	6 cut(s)	14, 88, 1894, 1952, 3137, 3277
PpuMI	RGGWCCY	1 cut(s)	994
PshBI	ATTAAT	2 cut(s)	2052, 2730
PsiI	TTATAA	2 cut(s)	716, 2873
Psp1406I	AACGTT	1 cut(s)	3173
Psp5II	RGGWCCY	1 cut(s)	994
PspFI	CCCAGC	1 cut(s)	2652
PspPI	GGNCC	4 cut(s)	54, 94, 994, 3274
PspPPI	RGGWCCY	1 cut(s)	994
PsrI	GAACNNNNNNTAC	2 cut(s)	2631, 2663
PstI	CTGCAG	2 cut(s)	736, 3305
PstNI	CAGNNNCTG	3 cut(s)	1280, 1445, 2282
PsuI	RGATCY	4 cut(s)	1725, 1733, 1880, 2233
PteI	GCGCGC	1 cut(s)	3307
PvuII	CAGCTG	2 cut(s)	839, 3240
RsaI	GTAC	9 cut(s)	275, 972, 1135, 2014, 2295, 2468, 3141, 3192, 3299
RsaNI	GTAC	9 cut(s)	274, 971, 1134, 2013, 2294, 2467, 3140, 3191, 3298
RseI	CAYNNNNRTG	1 cut(s)	1481
SapI	GCTCTTC	1 cut(s)	3066
Sau96I	GGNCC	4 cut(s)	54, 94, 994, 3274
ScaI	AGTACT	1 cut(s)	3299
SchI	GAGTC	6 cut(s)	14, 89, 1895, 1952, 3138, 3277
ScrFI	CCNGG	1 cut(s)	998
SduI	GDGCHC	6 cut(s)	1523, 1768, 2800, 2848, 2866, 2931
SfaNI	GCATC	5 cut(s)	1648, 2102, 2248, 2362, 2515
SfcI	CTRYAG	5 cut(s)	530, 732, 1386, 2823, 3301
SfoI	GGCGCC	1 cut(s)	88
SinI	GGWCC	4 cut(s)	54, 94, 994, 3274
SmiMI	CAYNNNNRTG	1 cut(s)	1481
SmlI	CTYRAG	1 cut(s)	676
SmoI	CTYRAG	1 cut(s)	676
SspDI	GGCGCC	1 cut(s)	86
SspI	AATATT	3 cut(s)	945, 1825, 2677
SspMI	CTAG	6 cut(s)	245, 507, 1047, 1371, 2204, 2792
StyD4I	CCNGG	1 cut(s)	996
StyI	CCWWGG	2 cut(s)	90, 2245
TaaI	ACNGT	7 cut(s)	931, 1276, 1500, 1579, 2707, 3187, 3330
TaiI	ACGT	7 cut(s)	50, 265, 281, 2459, 2472, 2662, 3176
TaqI	TCGA	8 cut(s)	50, 72, 117, 165, 265, 2025, 2085, 3061
TatI	WGTACW	3 cut(s)	2012, 3139, 3297
TauI	GCSGC	4 cut(s)	63, 242, 343, 2993
TfiI	GAWTC	9 cut(s)	14, 224, 1008, 1043, 1158, 1625, 1894, 2006, 2027
TscAI	CASTG	9 cut(s)	1446, 1546, 1693, 1773, 1950, 2268, 2311, 3094, 3192
TseFI	GTSAC	4 cut(s)	1420, 2302, 3094, 3130
Tsp45I	GTSAC	4 cut(s)	1420, 2302, 3094, 3130
TspDTI	ATGAA	9 cut(s)	382, 513, 618, 1056, 1877, 1889, 1958, 1998, 2154
TspGWI	ACGGA	4 cut(s)	795, 2393, 2399, 2495
TspRI	CASTG	9 cut(s)	1446, 1546, 1693, 1773, 1950, 2268, 2311, 3094, 3192
Van91I	CCANNNNNTGG	1 cut(s)	1712
VpaK11BI	GGWCC	4 cut(s)	54, 94, 994, 3274
VspI	ATTAAT	2 cut(s)	2052, 2730
XagI	CCTNNNNNAGG	2 cut(s)	1379, 2540
XapI	RAATTY	7 cut(s)	577, 1352, 1404, 2170, 2352, 2684, 3039
XmiI	GTMKAC	1 cut(s)	1495
XmnI	GAANNNNTTC	1 cut(s)	3083
XspI	CTAG	6 cut(s)	245, 507, 1047, 1371, 2204, 2792
ZraI	GACGTC	1 cut(s)	48
ZrmI	AGTACT	1 cut(s)	3299
Zsp2I	ATGCAT	1 cut(s)	2841

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.