Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0008228

Physical Location & Seq

Genomic Coordinates Forward (+)

65160847 .. 65174903

Gene Structure 14057 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020450

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3318 bp

ATGGCGTCCGGTGATTCCGACTCTTCCAAGAACGGCTTGCTGCCGATGTCGAGGTCCGGCCGCCAGTACCTCGATATGGAGTCCGGCGCCAAGGTCCGGCTATCCTCCGAGTTCGTCGGCGACAAGGCGTCCAAAAGCGACGATCACCACCACGACGACGACGACGACCCCTTCGACATTGCCCACACCAAGAACGCGCCTCCCGCCACGCTACGCCGATGGAGGCAAGCCGCGCTAGTGTTAAATGCTTCACGTCGATTTCGGTACACGTTGGACTTAAAAAAGGAAGAAGAGAAAGACAGTAGAAGGCGGATGATCAGATCCCATGCACAAGTCATAAGAGCCGCATTGCTTTTCAAATTGGCTGGAGAAAGGGAAATTGGGTTAGGCACAAAAGTCACACCTCCAACTCCAAGCGGTGATTATGGAATTGGACTTGAACCACTTGTTTCACTAACTAGGGAGCATAATACTTCCGCTCTACAGCAATATGGAGGGGTTAAAGGTATATCATCTTTGATAAAAACAAATTTGGAGAAGGGAATTGATGGGGATGAAGCAGATTTAATATATCGCAGAAATATGTTTGGATCAAATACATATCCTCAGAAAAAAGGGCGGAGTTTCTTGAGGTTCCTTTGGGAAGCATGGCAAGATTTAACTCTTATAATCTTGATAATAGCTGCAGCGGTGTCATTGGCACTTGGAATAAAAACAGAGGGTTTGGCTCACGGATGGTATGATGGAGGAAGCATTGCATTTGCTGTTTTTCTTGTTATCATTGTTACAGCTGTTAGTGATTATCGGCAATCTCTTCAGTTTCAAAATCTAAATGAGGAAAAGCAAAATATACAATTAGAGGTCATGAGAGGCGGTAGAACAGTAAAGATTTCAATATTTGATATTGTGGTTGGTGATGTTGTACCTCTCAGAATAGGTGATCAGGTCCCGGCTGATGGAATCTTAATCACCGGTCATTCTCTTGCGATTGATGAATCTAGTATGACCGGAGAGAGCAAAATTGTTCACAAGGATCAGAAAGCACCATTCTTAATGTCTGGCTGCAAAGTAGCTGATGGAGTGGGTACCATGCTGGTAACGAGTGTTGGAATCAATACAGAATGGGGATTGTTGATGGCAAGTATCTCTGAGGATAATGGTGAAGAAACTCCTTTACAGGTGCGCTTAAATGGACTTGCGACTTTTATAGGCATAGTTGGGCTTACAGTAGCTGCTTTGGTGCTTCTTGTTCTTTGGTTCAGATACTTTACTGGACATTCAAAAAATACAGATGGAAGTGTGGAATTTCAAGCGGGAAAGACTAGCCTTAGTAAGGCTGTAGATGGAGCTGTAAAAATTTTCACCATTGCTGTCACCATTGTCGTTGTTGCAGTGCCTGAAGGGCTACCTTTGGCTGTTACCTTGACGCTAGCATACTCGATGAGGAAAATGATGGCGGATAAGGCATTGGTGCGTAGACTTTCGGCTTGTGAAACTATGGGCTCAGCAACAACAATTTGCAGTGATAAAACTGGCACATTGACTTTAAATCAGATGACTGTGGTTGAGGCATATGTCGGGAGAAACAAGATACATCCACCAGATGATTCCTCACAGTTGCATCCACTGGTTTCCACTCTGCTAAGTGAGGGCATTGCACAGAATACCAGTGGGAATGTGTTTGAGCCTGAGGGCGGTGAAGTAGAGATCTCTGGATCTCCCACAGAAAAGGCTATACTTTCGTGGGCAGTGAAGTTGGGAATGAAGTTTAATGCTATCAGATCCGAGTCTATGATTCTTCATGTGTTCCCTTTCAACTCAGAGAAAAAGCGAGGTGGTGTTGCACTGAAAGGGTCTGACTCTGAAGTTCATATACATTGGAAGGGCGCTGCGGAGATAGTTTTAGATTCATGTACAGAGTATCTCGATTCAAATGGGTGTTCGCAGGACATTAATCAAGATAAGGATTTTTTCAAGGCAGCTATTGATGGCATGGCAGCAAGCAGTTTGCGATGCGTTGCCATTGCATACAGACCATATGAATTGGACAAAGTTCCTACTGAGGAAGAGAATTTAAGTCAATGGGCTTTACCTGAAGATAATCTAGTGTTGCTTGCTATCGTGGGTATAAAGGATCCTTGTCGCCTTGGGGTCAAAGATGCAGTGAGACTATGCACAGAGGCTGGTGTTAAGGTACGCATGGTCACTGGAGACAACCTTCAGACAGCAAAAGCAATCGCATTAGAGTGTGGAATTCTTTGTTCTGTTGAGGATGCTGCTGAGCCTAATATCATTGAAGGAAAAACATTCCGTGAATTATCTGAAAAAGAAAGAGAAGAAGTTGCCCAGAAAATAACGGTAATGGGGAGATCTTCCCCTAATGACAAGTTATTGCTTGTGCAAGCATTACGGAAGGGGGGAGATGTTGTTGCAGTAACAGGAGATGGCACTAATGATGCTCCTGCCCTTCACGAGGCAGATATTGGTCTATCTATGGGCATTCAAGGAACTGAAGTTGCAAAAGAAAGTTCGGATATCATTATCTTGGATGATAATTTTGCTTCTGTTGTAAAGGTTGTTCGCTGGGGACGTTCTGTGTATGCAAATATTCAGAAATTTATCCAATTCCAGCTTACGGTTAATGTTGCTGCTCTTACAATTAATGTTGTTGCGGCAATATCCTCTGGTAGTGTTCCTTTAAATGCTGTACAGCTTCTGTGGGTTAACTTGATTATGGATACTCTAGGAGCACTTGCATTGGCAACTGAACCACCTACGGACGACCTTATGCATAGAGCACCAGTTGGCAGAAGGGCACCGCTTATAACCAACATCATGTGGAGGAACCTACTAATTCAGGCTTTGTATCAAGTTTGTGTGCTCCTTGTCCTCAACTTTTTGGGTAATAGCATTCTTGGTCTGAAAGAGGACAACACACAAGCCGCTGGTGTGAAGAATACAATCATTTTCAATGCTTTTGTCTTCTGCCAAATTTTCAATGAGTTTAATGCTCGAAAGCCTGAAGAGCTGAATGTTTTCAGTGGAGTGACTAAGAACTACCTTTTCATGGGAATAATAGGAGTCACATTTGTACTTCAGATACTCATCATTCAGTTTCTTGGAACGTTCACAAAAACAGTGGTACTTGATTGGAAGCAATGGCTTATTTGCTTTGGTATTGCCATCATCAGCTGGCCTCTTGCTATTGTCGGCAAACTAATTCCGGTCCCTGAGACTCCATTCTCCCAGTACTGCAGGCGCGCATTTAAGCCATGCAGACAGCCATTTCAGCGAAGCAGAACTTCTCACAATACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007275 GO:0007338 GO:0008150 GO:0008324 GO:0009555 GO:0009566 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019953 GO:0022414 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044703 GO:0046873 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0051704 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1106

Amino Acids

120.6

Weight (kDa)

6.13

Isoelectric Point (pI)

39.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	55 - 98	8.5e-16	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	164 - 232	4.3e-13	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	285 - 381	2.7e-19	P-type ATPase actuator domain
Hydrolase	PF00702	505 - 827	6.1e-14	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	545 - 716	2.3e-30	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	898 - 1074	1.8e-45	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008228)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21180
fragaria_vesca	FvH4_6g37190 FvH4_6g37190 FvH4_6g37190 FvH4_6g37190
malus_domestica	MD09G1160300.v1.1 MD17G1146100.v1.1
prunus_persica	Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1
pyrus_communis	pycom09g07890 pycom17g13890
rosa_chinensis	RchiOBHm_Chr2g0149841
rosa_laevigata	RLG00000020450
rosa_multiflora	Rmu_ssc0000308.1_g000029
rosa_roxburghii	Rroxscaffold_2G00097600
rosa_rugosa	Rorug02G0417000
rosa_samantha	Rh2BG487700 Rh2CG461600 Rh2DG496500
rosa_wichuraiana	Rw2G038820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	668, 2825
Acc65I	GGTACC	1 cut(s)	1085
AccB1I	GGYRCC	3 cut(s)	86, 1085, 2815
AccBSI	CCGCTC	1 cut(s)	479
AccI	GTMKAC	1 cut(s)	1477
AccII	CGCG	3 cut(s)	197, 233, 3261
AclI	AACGTT	1 cut(s)	3125
AclWI	GGATC	7 cut(s)	315, 598, 1041, 1723, 1776, 2129, 2142
AcoI	YGGCCR	1 cut(s)	58
AcsI	RAATTY	7 cut(s)	529, 1304, 1356, 2071, 2253, 2615, 2991
AcuI	CTGAAG	8 cut(s)	800, 1419, 1884, 2115, 2204, 2532, 3042, 3080
AcyI	GRCGYC	3 cut(s)	5, 87, 128
AfiI	CCNNNNNNNGG	8 cut(s)	76, 96, 956, 1333, 1694, 2473, 2635, 3067
AflIII	ACRYGT	1 cut(s)	267
AgeI	ACCGGT	1 cut(s)	971
AhdI	GACNNNNNGTC	1 cut(s)	127
AjiI	CACGTC	1 cut(s)	254
AjuI	GAANNNNNNNTTGG	2 cut(s)	363, 395
Alw21I	GWGCWC	3 cut(s)	2752, 2800, 2883
Alw26I	GTCTC	3 cut(s)	2161, 2205, 3227
AlwI	GGATC	7 cut(s)	315, 598, 1041, 1723, 1776, 2129, 2142
AlwNI	CAGNNNCTG	4 cut(s)	1232, 1397, 2183, 2716
AoxI	GGCC	2 cut(s)	58, 3194
ApoI	RAATTY	7 cut(s)	529, 1304, 1356, 2071, 2253, 2615, 2991
ArsI	GACNNNNNNTTYG	2 cut(s)	155, 187
AseI	ATTAAT	2 cut(s)	1953, 2661
AsiGI	ACCGGT	1 cut(s)	971
Asp700I	GAANNNNTTC	1 cut(s)	3035
Asp718I	GGTACC	1 cut(s)	1085
AspLEI	GCGC	7 cut(s)	89, 199, 235, 1185, 1889, 3261, 3263
AspS9I	GGNCC	4 cut(s)	54, 94, 946, 3226
AsuC2I	CCSGG	1 cut(s)	950
AsuNHI	GCTAGC	1 cut(s)	1429
AvaII	GGWCC	4 cut(s)	54, 94, 946, 3226
AxyI	CCTNAGG	1 cut(s)	1689
BaeGI	GKGCMC	1 cut(s)	2818
BamHI	GGATCC	1 cut(s)	2134
BanI	GGYRCC	3 cut(s)	86, 1085, 2815
BanII	GRGCYC	1 cut(s)	1505
BarI	GAAGNNNNNNTAC	2 cut(s)	1857, 1889
BauI	CACGAG	1 cut(s)	2471
BbsI	GAAGAC	1 cut(s)	2974
Bbv12I	GWGCWC	3 cut(s)	2752, 2800, 2883
BceAI	ACGGC	1 cut(s)	49
BciVI	GTATCC	1 cut(s)	2731
BclI	TGATCA	2 cut(s)	315, 940
BcnI	CCSGG	1 cut(s)	950
BcoDI	GTCTC	3 cut(s)	2161, 2205, 3227
BfaI	CTAG	7 cut(s)	236, 459, 999, 1323, 1430, 2105, 2744
BfmI	CTRYAG	4 cut(s)	482, 684, 1338, 3253
BfoI	RGCGCY	2 cut(s)	90, 1890
BfuI	GTATCC	1 cut(s)	2731
BglI	GCCNNNNNGGC	1 cut(s)	1402
BglII	AGATCT	2 cut(s)	1707, 2369
BlpI	GCTNAGC	2 cut(s)	1504, 2280
BmcAI	AGTACT	1 cut(s)	3251
Bme1390I	CCNGG	1 cut(s)	950
Bme18I	GGWCC	4 cut(s)	54, 94, 946, 3226
BmeRI	GACNNNNNGTC	1 cut(s)	127
BmgBI	CACGTC	1 cut(s)	254
BmgT120I	GGNCC	4 cut(s)	54, 94, 946, 3226
BmiI	GGNNCC	8 cut(s)	88, 635, 948, 1087, 2136, 2817, 2846, 3228
BmrFI	CCNGG	1 cut(s)	950
BmrI	ACTGGG	1 cut(s)	3241
BmsI	GCATC	5 cut(s)	1630, 2003, 2149, 2263, 2446
BmtI	GCTAGC	1 cut(s)	1433
BmuI	ACTGGG	1 cut(s)	3241
BpiI	GAAGAC	1 cut(s)	2974
BpmI	CTGGAG	2 cut(s)	387, 2229
Bpu1102I	GCTNAGC	2 cut(s)	1504, 2280
BpuEI	CTTGAG	1 cut(s)	649
BpuMI	CCSGG	1 cut(s)	950
BsaBI	GATNNNNATC	1 cut(s)	3104
BsaHI	GRCGYC	3 cut(s)	5, 87, 128
BsaJI	CCNNGG	2 cut(s)	90, 2146
BsaWI	WCCGGW	4 cut(s)	8, 971, 1007, 3223
BsaXI	ACNNNNNCTCC	2 cut(s)	2412, 2442
Bsc4I	CCNNNNNNNGG	8 cut(s)	76, 96, 956, 1333, 1694, 2473, 2635, 3067
Bse118I	RCCGGY	1 cut(s)	971
Bse1I	ACTGG	8 cut(s)	64, 1276, 1537, 1632, 1668, 2212, 2801, 3247
Bse21I	CCTNAGG	1 cut(s)	1689
Bse3DI	GCAATG	7 cut(s)	177, 347, 753, 1365, 1653, 2022, 3164
Bse8I	GATNNNNATC	1 cut(s)	3104
BseDI	CCNNGG	2 cut(s)	90, 2146
BseGI	GGATG	7 cut(s)	318, 559, 740, 1594, 1621, 2278, 2554
BseJI	GATNNNNATC	1 cut(s)	3104
BseLI	CCNNNNNNNGG	8 cut(s)	76, 96, 956, 1333, 1694, 2473, 2635, 3067
BseMI	GCAATG	7 cut(s)	177, 347, 753, 1365, 1653, 2022, 3164
BseMII	CTCAG	9 cut(s)	620, 943, 1140, 1518, 1680, 1833, 2052, 2271, 3222
BseNI	ACTGG	8 cut(s)	64, 1276, 1537, 1632, 1668, 2212, 2801, 3247
BsePI	GCGCGC	1 cut(s)	3259
BseSI	GKGCMC	1 cut(s)	2818
BseX3I	CGGCCG	1 cut(s)	58
BseYI	CCCAGC	1 cut(s)	2583
Bsh1236I	CGCG	3 cut(s)	197, 233, 3261
Bsh1285I	CGRYCG	1 cut(s)	61
BshFI	GGCC	2 cut(s)	60, 3196
BshNI	GGYRCC	3 cut(s)	86, 1085, 2815
BshTI	ACCGGT	1 cut(s)	971
BsiEI	CGRYCG	1 cut(s)	61
BsiHKAI	GWGCWC	3 cut(s)	2752, 2800, 2883
BsiSI	CCGG	8 cut(s)	9, 57, 84, 97, 950, 972, 1008, 3224
BslFI	GGGAC	3 cut(s)	932, 2601, 3212
BslI	CCNNNNNNNGG	8 cut(s)	76, 96, 956, 1333, 1694, 2473, 2635, 3067
BsmAI	GTCTC	3 cut(s)	2161, 2205, 3227
BsmFI	GGGAC	3 cut(s)	932, 2601, 3212
BsmI	GAATGC	2 cut(s)	2499, 2910
BsnI	GGCC	2 cut(s)	60, 3196
Bsp1286I	GDGCHC	5 cut(s)	1505, 2752, 2800, 2818, 2883
Bsp1407I	TGTACA	2 cut(s)	1913, 2707
Bsp1720I	GCTNAGC	2 cut(s)	1504, 2280
BspANI	GGCC	2 cut(s)	60, 3196
BspCNI	CTCAG	9 cut(s)	619, 942, 1141, 1517, 1681, 1832, 2053, 2272, 3223
BspFNI	CGCG	3 cut(s)	197, 233, 3261
BspHI	TCATGA	1 cut(s)	864
BspLI	GGNNCC	8 cut(s)	88, 635, 948, 1087, 2136, 2817, 2846, 3228
BspMAI	CTGCAG	2 cut(s)	688, 3257
BspOI	GCTAGC	1 cut(s)	1433
BspPI	GGATC	7 cut(s)	315, 598, 1041, 1723, 1776, 2129, 2142
BspQI	GCTCTTC	1 cut(s)	3018
BspT107I	GGYRCC	3 cut(s)	86, 1085, 2815
BsrBI	CCGCTC	1 cut(s)	479
BsrDI	GCAATG	7 cut(s)	177, 347, 753, 1365, 1653, 2022, 3164
BsrFI	RCCGGY	1 cut(s)	971
BsrGI	TGTACA	2 cut(s)	1913, 2707
BsrI	ACTGG	8 cut(s)	64, 1276, 1537, 1632, 1668, 2212, 2801, 3247
BssAI	RCCGGY	1 cut(s)	971
BssECI	CCNNGG	2 cut(s)	90, 2146
BssHII	GCGCGC	1 cut(s)	3259
BssNI	GRCGYC	3 cut(s)	5, 87, 128
BssSI	CACGAG	1 cut(s)	2471
BssT1I	CCWWGG	2 cut(s)	90, 2146
Bst2BI	CACGAG	1 cut(s)	2471
Bst4CI	ACNGT	8 cut(s)	302, 883, 1228, 1561, 1617, 2359, 2638, 3139
Bst6I	CTCTTC	5 cut(s)	28, 285, 819, 2061, 3018
BstACI	GRCGYC	3 cut(s)	5, 87, 128
BstAUI	TGTACA	2 cut(s)	1913, 2707
BstC8I	GCNNGC	9 cut(s)	38, 228, 1431, 2002, 2115, 2403, 3194, 3257, 3261
BstENI	CCTNNNNNAGG	2 cut(s)	1331, 2471
BstF5I	GGATG	7 cut(s)	318, 559, 740, 1594, 1621, 2278, 2554
BstFNI	CGCG	3 cut(s)	197, 233, 3261
BstH2I	RGCGCY	2 cut(s)	90, 1890
BstHHI	GCGC	7 cut(s)	89, 199, 235, 1185, 1889, 3261, 3263
BstMAI	GTCTC	3 cut(s)	2161, 2205, 3227
BstMCI	CGRYCG	1 cut(s)	61
BstMWI	GCNNNNNNNGC	6 cut(s)	203, 232, 1402, 1463, 3025, 3289
BstSCI	CCNGG	1 cut(s)	948
BstSFI	CTRYAG	4 cut(s)	482, 684, 1338, 3253
BstSLI	GKGCMC	1 cut(s)	2818
BstUI	CGCG	3 cut(s)	197, 233, 3261
BstV2I	GAAGAC	1 cut(s)	2974
BstX2I	RGATCY	6 cut(s)	320, 1707, 1715, 1781, 2134, 2369
BstYI	RGATCY	6 cut(s)	320, 1707, 1715, 1781, 2134, 2369
BstZI	CGGCCG	1 cut(s)	58
Bsu36I	CCTNAGG	1 cut(s)	1689
BsuI	GTATCC	1 cut(s)	2731
BsuRI	GGCC	2 cut(s)	60, 3196
BtgZI	GCGATG	1 cut(s)	2026
BtrI	CACGTC	1 cut(s)	254
BtsCI	GGATG	7 cut(s)	318, 559, 740, 1594, 1621, 2278, 2554
BtsI	GCAGTG	4 cut(s)	1398, 1528, 1755, 2169
Cac8I	GCNNGC	9 cut(s)	38, 228, 1431, 2002, 2115, 2403, 3194, 3257, 3261
CaiI	CAGNNNCTG	4 cut(s)	1232, 1397, 2183, 2716
CciI	TCATGA	1 cut(s)	864
CfoI	GCGC	7 cut(s)	89, 199, 235, 1185, 1889, 3261, 3263
Cfr10I	RCCGGY	1 cut(s)	971
Cfr13I	GGNCC	4 cut(s)	54, 94, 946, 3226
CseI	GACGC	2 cut(s)	117, 1435
CspAI	ACCGGT	1 cut(s)	971
CspCI	CAANNNNNGTGG	2 cut(s)	3120, 3155
DinI	GGCGCC	1 cut(s)	88
DraI	TTTAAA	2 cut(s)	1548, 2700
DriI	GACNNNNNGTC	1 cut(s)	127
EaeI	YGGCCR	1 cut(s)	58
EagI	CGGCCG	1 cut(s)	58
Eam1104I	CTCTTC	5 cut(s)	28, 285, 819, 2061, 3018
Eam1105I	GACNNNNNGTC	1 cut(s)	127
EarI	CTCTTC	5 cut(s)	28, 285, 819, 2061, 3018
EciI	GGCGGA	3 cut(s)	325, 634, 1472
EclXI	CGGCCG	1 cut(s)	58
Eco130I	CCWWGG	2 cut(s)	90, 2146
Eco24I	GRGCYC	1 cut(s)	1505
Eco32I	GATATC	1 cut(s)	2536
Eco47I	GGWCC	4 cut(s)	54, 94, 946, 3226
Eco52I	CGGCCG	1 cut(s)	58
Eco57I	CTGAAG	8 cut(s)	800, 1419, 1884, 2115, 2204, 2532, 3042, 3080
Eco81I	CCTNAGG	1 cut(s)	1689
EcoNI	CCTNNNNNAGG	2 cut(s)	1331, 2471
EcoO109I	RGGNCCY	1 cut(s)	946
EcoRI	GAATTC	1 cut(s)	2253
EcoRV	GATATC	1 cut(s)	2536
EcoT14I	CCWWGG	2 cut(s)	90, 2146
EcoT22I	ATGCAT	1 cut(s)	2793
EcoT38I	GRGCYC	1 cut(s)	1505
EgeI	GGCGCC	1 cut(s)	88
EheI	GGCGCC	1 cut(s)	88
ErhI	CCWWGG	2 cut(s)	90, 2146
FalI	AAGNNNNNCTT	4 cut(s)	20, 52, 3286, 3318
FaqI	GGGAC	3 cut(s)	932, 2601, 3212
FauI	CCCGC	2 cut(s)	211, 1306
FauNDI	CATATG	2 cut(s)	1573, 2038
FbaI	TGATCA	2 cut(s)	315, 940
FblI	GTMKAC	1 cut(s)	1477
FokI	GGATG	7 cut(s)	325, 566, 747, 1581, 1608, 2285, 2561
FriOI	GRGCYC	1 cut(s)	1505
FspBI	CTAG	7 cut(s)	236, 459, 999, 1323, 1430, 2105, 2744
GlaI	GCGC	7 cut(s)	88, 198, 234, 1184, 1888, 3260, 3262
GsaI	CCCAGC	1 cut(s)	2587
GsuI	CTGGAG	2 cut(s)	387, 2229
HaeII	RGCGCY	2 cut(s)	90, 1890
HaeIII	GGCC	2 cut(s)	60, 3196
HapII	CCGG	8 cut(s)	9, 57, 84, 97, 950, 972, 1008, 3224
HgaI	GACGC	2 cut(s)	117, 1435
HhaI	GCGC	7 cut(s)	89, 199, 235, 1185, 1889, 3261, 3263
Hin1I	GRCGYC	3 cut(s)	5, 87, 128
Hin6I	GCGC	7 cut(s)	87, 197, 233, 1183, 1887, 3259, 3261
HinP1I	GCGC	7 cut(s)	87, 197, 233, 1183, 1887, 3259, 3261
HincII	GTYRAC	1 cut(s)	2725
HindII	GTYRAC	1 cut(s)	2725
HpaI	GTTAAC	1 cut(s)	2725
HpaII	CCGG	8 cut(s)	9, 57, 84, 97, 950, 972, 1008, 3224
Hpy166II	GTNNAC	5 cut(s)	267, 1027, 1478, 2725, 3129
Hpy188III	TCNNGA	8 cut(s)	628, 673, 865, 1579, 1713, 1925, 1958, 2471
Hpy8I	GTNNAC	5 cut(s)	267, 1027, 1478, 2725, 3129
Hpy99I	CGWCG	7 cut(s)	119, 143, 158, 161, 164, 167, 258
HpyCH4III	ACNGT	8 cut(s)	302, 883, 1228, 1561, 1617, 2359, 2638, 3139
HpyCH4IV	ACGT	4 cut(s)	253, 269, 2590, 3125
HpyF10VI	GCNNNNNNNGC	6 cut(s)	203, 232, 1402, 1463, 3025, 3289
HpySE526I	ACGT	4 cut(s)	253, 269, 2590, 3125
Hsp92I	GRCGYC	3 cut(s)	5, 87, 128
HspAI	GCGC	7 cut(s)	87, 197, 233, 1183, 1887, 3259, 3261
KasI	GGCGCC	1 cut(s)	86
KpnI	GGTACC	1 cut(s)	1089
Ksp22I	TGATCA	2 cut(s)	315, 940
KspAI	GTTAAC	1 cut(s)	2725
LguI	GCTCTTC	1 cut(s)	3018
LmnI	GCTCC	5 cut(s)	463, 1346, 2464, 2747, 2886
LweI	GCATC	5 cut(s)	1630, 2003, 2149, 2263, 2446
MaeI	CTAG	7 cut(s)	236, 459, 999, 1323, 1430, 2105, 2744
MaeII	ACGT	4 cut(s)	253, 269, 2590, 3125
MaeIII	GTNAC	9 cut(s)	397, 784, 1096, 1372, 1417, 2203, 2434, 3046, 3082
MbiI	CCGCTC	1 cut(s)	479
MflI	RGATCY	6 cut(s)	320, 1707, 1715, 1781, 2134, 2369
MhlI	GDGCHC	5 cut(s)	1505, 2752, 2800, 2818, 2883
Mly113I	GGCGCC	1 cut(s)	87
MlyI	GAGTC	6 cut(s)	14, 89, 1796, 1853, 3090, 3229
MmeI	TCCRAC	4 cut(s)	42, 252, 431, 1087
Mph1103I	ATGCAT	1 cut(s)	2793
MroXI	GAANNNNTTC	1 cut(s)	3035
MslI	CAYNNNNRTG	1 cut(s)	2245
MspA1I	CMGCKG	4 cut(s)	689, 791, 2945, 3192
MspI	CCGG	8 cut(s)	9, 57, 84, 97, 950, 972, 1008, 3224
MspR9I	CCNGG	1 cut(s)	950
Mva1269I	GAATGC	2 cut(s)	2499, 2910
MvnI	CGCG	3 cut(s)	197, 233, 3261
MwoI	GCNNNNNNNGC	6 cut(s)	203, 232, 1402, 1463, 3025, 3289
NarI	GGCGCC	1 cut(s)	87
NciI	CCSGG	1 cut(s)	950
NdeI	CATATG	2 cut(s)	1573, 2038
NheI	GCTAGC	1 cut(s)	1429
NlaIV	GGNNCC	8 cut(s)	88, 635, 948, 1087, 2136, 2817, 2846, 3228
NmuCI	GTSAC	5 cut(s)	397, 1372, 2203, 3046, 3082
NsiI	ATGCAT	1 cut(s)	2793
PagI	TCATGA	1 cut(s)	864
PauI	GCGCGC	1 cut(s)	3259
PciSI	GCTCTTC	1 cut(s)	3018
PcsI	WCGNNNNNNNCGW	4 cut(s)	159, 162, 171, 2587
PctI	GAATGC	2 cut(s)	2499, 2910
PdmI	GAANNNNTTC	1 cut(s)	3035
PfeI	GAWTC	8 cut(s)	14, 960, 995, 1110, 1607, 1795, 1907, 1928
PinAI	ACCGGT	1 cut(s)	971
PleI	GAGTC	6 cut(s)	14, 88, 1795, 1853, 3089, 3229
PluTI	GGCGCC	1 cut(s)	90
PpsI	GAGTC	6 cut(s)	14, 88, 1795, 1853, 3089, 3229
PpuMI	RGGWCCY	1 cut(s)	946
PshBI	ATTAAT	2 cut(s)	1953, 2661
PsiI	TTATAA	2 cut(s)	668, 2825
Psp1406I	AACGTT	1 cut(s)	3125
Psp5II	RGGWCCY	1 cut(s)	946
PspFI	CCCAGC	1 cut(s)	2583
PspN4I	GGNNCC	8 cut(s)	88, 635, 948, 1087, 2136, 2817, 2846, 3228
PspPI	GGNCC	4 cut(s)	54, 94, 946, 3226
PspPPI	RGGWCCY	1 cut(s)	946
PsrI	GAACNNNNNNTAC	2 cut(s)	2562, 2594
PstI	CTGCAG	2 cut(s)	688, 3257
PstNI	CAGNNNCTG	4 cut(s)	1232, 1397, 2183, 2716
PsuI	RGATCY	6 cut(s)	320, 1707, 1715, 1781, 2134, 2369
PteI	GCGCGC	1 cut(s)	3259
PvuII	CAGCTG	2 cut(s)	791, 3192
RseI	CAYNNNNRTG	1 cut(s)	2245
SapI	GCTCTTC	1 cut(s)	3018
Sau96I	GGNCC	4 cut(s)	54, 94, 946, 3226
ScaI	AGTACT	1 cut(s)	3251
SchI	GAGTC	6 cut(s)	14, 89, 1796, 1853, 3090, 3229
ScrFI	CCNGG	1 cut(s)	950
SduI	GDGCHC	5 cut(s)	1505, 2752, 2800, 2818, 2883
SfaNI	GCATC	5 cut(s)	1630, 2003, 2149, 2263, 2446
SfcI	CTRYAG	4 cut(s)	482, 684, 1338, 3253
SfoI	GGCGCC	1 cut(s)	88
SinI	GGWCC	4 cut(s)	54, 94, 946, 3226
SmiMI	CAYNNNNRTG	1 cut(s)	2245
SmlI	CTYRAG	1 cut(s)	628
SmoI	CTYRAG	1 cut(s)	628
SspDI	GGCGCC	1 cut(s)	86
SspI	AATATT	2 cut(s)	897, 2608
SspMI	CTAG	7 cut(s)	236, 459, 999, 1323, 1430, 2105, 2744
StyD4I	CCNGG	1 cut(s)	948
StyI	CCWWGG	2 cut(s)	90, 2146
TaaI	ACNGT	8 cut(s)	302, 883, 1228, 1561, 1617, 2359, 2638, 3139
TaiI	ACGT	4 cut(s)	256, 272, 2593, 3128
TaqI	TCGA	7 cut(s)	50, 72, 174, 256, 1439, 1926, 3013
TatI	WGTACW	4 cut(s)	1913, 2707, 3091, 3249
TauI	GCSGC	5 cut(s)	63, 233, 347, 2675, 2945
TfiI	GAWTC	8 cut(s)	14, 960, 995, 1110, 1607, 1795, 1907, 1928
TseFI	GTSAC	5 cut(s)	397, 1372, 2203, 3046, 3082
Tsp45I	GTSAC	5 cut(s)	397, 1372, 2203, 3046, 3082
TspDTI	ATGAA	8 cut(s)	570, 1008, 1778, 1790, 1859, 1899, 2055, 3055
TspGWI	ACGGA	4 cut(s)	747, 2300, 2425, 2792
VpaK11BI	GGWCC	4 cut(s)	54, 94, 946, 3226
VspI	ATTAAT	2 cut(s)	1953, 2661
XagI	CCTNNNNNAGG	2 cut(s)	1331, 2471
XapI	RAATTY	7 cut(s)	529, 1304, 1356, 2071, 2253, 2615, 2991
XmiI	GTMKAC	1 cut(s)	1477
XmnI	GAANNNNTTC	1 cut(s)	3035
XspI	CTAG	7 cut(s)	236, 459, 999, 1323, 1430, 2105, 2744
ZrmI	AGTACT	1 cut(s)	3251
Zsp2I	ATGCAT	1 cut(s)	2793

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.