Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0008228

Physical Location & Seq

Genomic Coordinates Reverse (-)

13184194 .. 13193442

Gene Structure 9249 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1146100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3312 bp

ATGGCCGCGTCGCGTGGTTCGTCGTCTTCCACGAACGGGTTGTTGCCGACGTCGTTGTCGGAGCGGAACCACCACGACATGGAGGCCGGGGGAAGGGGAGGAGGGGAAAGGGTGGGACTGGAGACCGAGTTCAACGACGACGACGCTGCCGCGGCGAACGACATGCCGACCGATCCCTTCGACATTGCCCACACCAAGAACGCGCCTCCCGCGACGCTCAGGAGATGGAGGCAAGCGGCACTAGTGTTAAATGCTTCCAGACGGTTCAGGTACACTATGGACTTGAAAGCGGAAGAAGAAAAAGAAAATAGCAGGCGGATGATTAGATCCCATGCACAAGTCATAAGAGCAGCATTGCTTTTCAGATTGGCAGGAGAACGGGAACTTGGGATTGGCACAACAGTTGCGCCCCCAACTCCCAGTGGTGATTATGGAATTGCACTAGACGAACTTGTTACATTGACCAGGGAGAACAACATTACCGCTTTACAGCAATATGGAGGGGTTAAAGGCATATCAGCGTTGCTAAAATCGAATGCGGAGAAGGGAATTGATGGGGTTGAAACAGATGTAGTAAAACGAAAGGAAGTGTTTGGATCAAATACATATCCTCGGAAAAAAGGGCGGAGTTTCTTGAGATTCCTTTGGGAAGCTTGGCAAGACTTAACTCTTATAATCTTGATCATAGCTGCAGTCGTGTCATTGGGACTCGGAATAAAAACTGAGGGTCTGGAGGAAGGATGGTATGATGGAGGAAGCATTTTCTTTGCTGTGTTTCTTGTTATAATTGTTACAGCTGTTAGTGATTATCGGCAATCTCTTCAGTTCCAAAATCTTAACTCGGAAAAACAAAATATACAACTGGAGGTCCTGAGAGGCGGTAGATCAGTAAAGATCTCAATATTTGATATTGTGGTTGGTGATGTTGTACCACTCAGAATAGGTGATCAAGTCCCTGCTGATGGAATCTTAGTCACTGGTCATTCACTTGCTATCGATGAATCTAGCATGACTGGTGAAAGCAAGATTGTTCACAAGGATCAGAAAGCACCTTTCTTAATGTCAGGTTGCAAAGTGGCTGATGGAGTGGGTACTATGCTGGTAACTGGTGTTGGGATCAATACTGAATGGGGATTGTTGATGGCAAGCATCTCAGAGGATACTGGTGAAGAAACTCCTTTACAGGTGCGTTTGAATGGAGTTGCAACTTTTATAGGCATAGTTGGGCTTTCAGTAGCTGTTTTGGTGCTTGCCGTCCTTTGGGCCAGATACTTCACTGGAAATACAAGAGATCTAGATGGAAAAGTCCAGTTTATAGCTGGTCAGACTAGAATTGGCAAAGCTTTTGATGGAGCAATAAAAATTTTCACGATTGCTGTCACCATTGTGGTTGTTGCAGTCCCTGAGGGGCTACCTTTGGCCGTTACCTTGACGCTAGCATACTCAATGAAGAAAATGATGGCAGATAAGGCATTGGTCCGTAGGCTGTCAGCCTGTGAAACTATGGGTTCAGCAACAACGATTTGCAGTGATAAAACTGGGACATTGACTTTAAATCAGATGACTGTGGTTGAGGCATATGTTGGGAGAAAGAAGATAAATCCACCAGATGATTCCTCACAGTTGCATCCACTGGTTTCCACTCTGTTAAGTGAGGGCGTTGCGCAGAATACCACTGGCAATGTATTTGAGCCAACGGGTGGTGAAGTAGAGATTTCTGGATCTCCTACGGAAAAGGCTATACTTTCTTGGGCATTCAAGTTGGGAATGAAGTTTGGTGTTATCAGATTGGAGTCTACAGTCCTTCATGTCTTCCCTTTCAACTCAGAGAAAAAGCGAGGTGGTGTTGCAGTAAAGCAGGCTGACTCTAATGTTCATATACATTGGAAAGGAGCAGCGGAGATACTTTTAGCCTCATGCACAGAGTACCTTGACTCAGATGGTTGCTTGCATAATATTAAAGATGACAAGGAATTTTTCGCGACAGCTATTAATGACATGGCAGAGAGCAGTTTGCGGTGTGTTGCCATTGCATATAGACCATATGGATTGGACAAAGTTCCTACCGATGAAGAGCAATTAAGTCAATGGGCATTACCAGAAGATAACCTTGTCCTGCTTGCTATTGTTGGCATAAAGGATCCTTGTCGTCCTGGTGTCAGAGATGCAGTGAAACAATGCACAGAAGCTGGTGTCAAGGTACGCATGGTCACTGGAGACAATCTTCAAACAGCAAAAGCGATCGCGTTAGAGTGTGGAATACTACTTTCACTTGAAGATGCTACTGAGCCTACTATCATTGAAGGAAAAGTATTCCGTGAATTGTCTGAAAGGGAAAGGGAACAACTCGCCAAGAAAATAACAGTAATGGGCAGATCTTCTCCCAATGACAAGTTGTTGCTTGTGCAAGCATTGCGTAAGGGAGGTGAAGTTGTTGCAGTAACAGGGGATGGCACTAATGATGCTCCGGCACTTCATGAGGCAGATATTGGTCTTTCTATGGGCATTCAAGGAACTGAAGTTGCTAAGGAAAGTTCGGATATCGTTATCTTGGATGATAATTTTGCGTCTGTTGTAAAGGTTGTTCGCTGGGGACGTTCTGTGTATGCAAATATTCAGAAATTTATCCAATTCCAACTTACTGTTAATGTTGCCGCTCTTGTAATTAATGTTGTGGCCGCAATCTCTTCTGGTCGTGTTCCTTTAAATGCAGTACAGCTTCTGTGGGTTAACTTGATCATGGATACTCTAGGAGCCCTTGCGTTGGCTACCGAACCACCTACAGACAACCTTATGCATAGAACACCAGTTGGCCGAAGGGCACCTCTTATAACGAACATCATGTGGAGGAACCTACTCATTCAGGCTATGTATCAAGTTACTGTCCTCCTTGTCCTCAACTTTTTGGGAAATAGCATTCTTGGTCTGCAGCAGGATGTCCCAAAGCATGCCGTTGGTGTGAAGAATACTGTCATATTCAATGCATTCGTCTTTTGTCAAATTTTCAACGAGTTCAATGCTCGAAAGCCTGAAGAAATAAATGTCTTCACTGGAGTGACCAAAAACTACCTCTTCATGGGAATAATAGGAATTACATTTGTACTTCAGATAATCATCGTCATGTTCCTCGGAAAATTTACAAAAACGGTTAGACTATCATGGCAGCAATGGCTCATATGCTTGGGAATTGCCATTGTCAGTTGGCCTCTTGCCATTATTGGAAAACTAATTCCGGTCCCAAAGACTCCGTTTTCCCAGTACTTCAGTCGGCCTATTCAGCGGTGCAGAAATTCTCGTAATCGTAATACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007275 GO:0007338 GO:0008150 GO:0008324 GO:0009555 GO:0009566 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0019953 GO:0022414 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044703 GO:0046873 GO:0048229 GO:0048856 GO:0051179 GO:0051234 GO:0051704 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1104

Amino Acids

120.13

Weight (kDa)

6.94

Isoelectric Point (pI)

37.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CaATP_NAI	PF12515	58 - 100	4e-14	Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N	PF00690	166 - 234	5.2e-15	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	287 - 383	4.8e-19	P-type ATPase actuator domain
Hydrolase	PF00702	507 - 829	1.1e-15	haloacid dehalogenase-like hydrolase
Cation_ATPase	PF13246	547 - 718	4.4e-28	P-type ATPase, cytoplasmic domain N
Cation_ATPase_C	PF00689	900 - 1077	3.1e-45	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008228)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G21180
fragaria_vesca	FvH4_6g37190 FvH4_6g37190 FvH4_6g37190 FvH4_6g37190
malus_domestica	MD09G1160300.v1.1 MD17G1146100.v1.1
prunus_persica	Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1 Prupe.3G018900_v2.0.a1
pyrus_communis	pycom09g07890 pycom17g13890
rosa_chinensis	RchiOBHm_Chr2g0149841
rosa_laevigata	RLG00000020450
rosa_multiflora	Rmu_ssc0000308.1_g000029
rosa_roxburghii	Rroxscaffold_2G00097600
rosa_rugosa	Rorug02G0417000
rosa_samantha	Rh2BG487700 Rh2CG461600 Rh2DG496500
rosa_wichuraiana	Rw2G038820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	674, 785, 2831
AasI	GACNNNNNNGTC	1 cut(s)	55
AatII	GACGTC	1 cut(s)	53
Acc16I	TGCGCA	1 cut(s)	1665
AccB1I	GGYRCC	1 cut(s)	2821
AccB7I	CCANNNNNTGG	2 cut(s)	79, 1700
AccBSI	CCGCTC	2 cut(s)	64, 2657
AccI	GTMKAC	1 cut(s)	1796
AccII	CGCG	7 cut(s)	8, 13, 152, 203, 212, 1982, 2246
AclWI	GGATC	8 cut(s)	167, 321, 604, 1047, 1124, 1729, 2135, 2148
AcoI	YGGCCR	4 cut(s)	3, 1419, 2676, 2812
AcsI	RAATTY	6 cut(s)	1362, 1973, 2621, 3000, 3134, 3289
AcuI	CTGAAG	5 cut(s)	806, 2538, 3051, 3089, 3247
AcyI	GRCGYC	1 cut(s)	50
AfaI	GTAC	8 cut(s)	272, 930, 1093, 1928, 2202, 2715, 3102, 3260
AfiI	CCNNNNNNNGG	8 cut(s)	36, 79, 93, 962, 1260, 1700, 2764, 3076
AhlI	ACTAGT	1 cut(s)	241
AjnI	CCWGG	2 cut(s)	464, 2152
AjuI	GAANNNNNNNTTGG	4 cut(s)	369, 375, 401, 407
AleI	CACNNNNGTG	2 cut(s)	1385, 3053
AloI	GAACNNNNNNTCC	2 cut(s)	113, 145
AluBI	AGCT	9 cut(s)	653, 689, 797, 1238, 1319, 1343, 1988, 2189, 2719
AluI	AGCT	9 cut(s)	653, 689, 797, 1238, 1319, 1343, 1988, 2189, 2719
Alw26I	GTCTC	2 cut(s)	116, 2211
AlwI	GGATC	8 cut(s)	167, 321, 604, 1047, 1124, 1729, 2135, 2148
AlwNI	CAGNNNCTG	4 cut(s)	1238, 1403, 2189, 2722
AoxI	GGCC	8 cut(s)	3, 84, 1263, 1419, 2676, 2812, 3203, 3269
ApeKI	GCWGC	6 cut(s)	146, 350, 689, 1895, 2929, 3163
ApoI	RAATTY	6 cut(s)	1362, 1973, 2621, 3000, 3134, 3289
AseI	ATTAAT	2 cut(s)	1992, 2667
AsiSI	GCGATCGC	1 cut(s)	2244
AspLEI	GCGC	3 cut(s)	205, 409, 1666
AspS9I	GGNCC	4 cut(s)	868, 1263, 1477, 3235
AsuC2I	CCSGG	1 cut(s)	88
AsuHPI	GGTGA	8 cut(s)	437, 932, 956, 1028, 1178, 1372, 1715, 2438
AsuNHI	GCTAGC	1 cut(s)	1435
AvaII	GGWCC	3 cut(s)	868, 1477, 3235
AxyI	CCTNAGG	1 cut(s)	1404
BaeGI	GKGCMC	1 cut(s)	2824
BamHI	GGATCC	1 cut(s)	2140
BanI	GGYRCC	1 cut(s)	2821
BanII	GRGCYC	1 cut(s)	2758
BbsI	GAAGAC	3 cut(s)	18, 1804, 3037
BbvI	GCAGC	6 cut(s)	133, 362, 676, 1907, 2941, 3175
BceAI	ACGGC	3 cut(s)	1238, 1406, 2936
BcgI	CGANNNNNNTGC	6 cut(s)	128, 145, 162, 179, 2999, 3033
BciT130I	CCWGG	2 cut(s)	466, 2154
BciVI	GTATCC	2 cut(s)	1153, 2737
BclI	TGATCA	3 cut(s)	681, 946, 2736
BcnI	CCSGG	1 cut(s)	88
BcoDI	GTCTC	2 cut(s)	116, 2211
BcuI	ACTAGT	1 cut(s)	241
BfaI	CTAG	7 cut(s)	242, 443, 1005, 1295, 1329, 1436, 2750
BfmI	CTRYAG	4 cut(s)	690, 1797, 2781, 2927
BfuI	GTATCC	2 cut(s)	1153, 2737
BglII	AGATCT	3 cut(s)	894, 1291, 2375
BmcAI	AGTACT	1 cut(s)	3260
Bme1390I	CCNGG	3 cut(s)	88, 466, 2154
Bme18I	GGWCC	3 cut(s)	868, 1477, 3235
BmgT120I	GGNCC	4 cut(s)	868, 1263, 1477, 3235
BmiI	GGNNCC	6 cut(s)	68, 2142, 2755, 2823, 2852, 3237
BmrFI	CCNGG	3 cut(s)	88, 466, 2154
BmrI	ACTGGG	3 cut(s)	414, 1548, 3250
BmsI	GCATC	5 cut(s)	1158, 1636, 2155, 2269, 2452
BmtI	GCTAGC	1 cut(s)	1439
BmuI	ACTGGG	3 cut(s)	414, 1548, 3250
BpiI	GAAGAC	3 cut(s)	18, 1804, 3037
BpmI	CTGGAG	5 cut(s)	140, 752, 884, 2235, 3072
Bpu10I	CCTNAGC	2 cut(s)	218, 2526
BpuEI	CTTGAG	1 cut(s)	655
BpuMI	CCSGG	1 cut(s)	88
Bsa29I	ATCGAT	1 cut(s)	996
BsaBI	GATNNNNATC	1 cut(s)	3113
BsaHI	GRCGYC	1 cut(s)	50
BsaI	GGTCTC	1 cut(s)	116
BsaJI	CCNNGG	5 cut(s)	87, 150, 465, 611, 3127
BsaWI	WCCGGW	1 cut(s)	3232
BsaXI	ACNNNNNCTCC	4 cut(s)	113, 143, 2415, 2445
Bsc4I	CCNNNNNNNGG	8 cut(s)	36, 79, 93, 962, 1260, 1700, 2764, 3076
Bse21I	CCTNAGG	1 cut(s)	1404
Bse3DI	GCAATG	6 cut(s)	183, 353, 1687, 2028, 2411, 3173
Bse8I	GATNNNNATC	1 cut(s)	3113
BseBI	CCWGG	2 cut(s)	466, 2154
BseCI	ATCGAT	1 cut(s)	996
BseDI	CCNNGG	5 cut(s)	87, 150, 465, 611, 3127
BseGI	GGATG	6 cut(s)	324, 746, 1627, 2455, 2560, 2941
BseJI	GATNNNNATC	1 cut(s)	3113
BseLI	CCNNNNNNNGG	8 cut(s)	36, 79, 93, 962, 1260, 1700, 2764, 3076
BseMI	GCAATG	6 cut(s)	183, 353, 1687, 2028, 2411, 3173
BseMII	CTCAG	9 cut(s)	232, 714, 863, 949, 1167, 1395, 1839, 1950, 2277
BseRI	GAGGAG	1 cut(s)	114
BseSI	GKGCMC	1 cut(s)	2824
BseXI	GCAGC	6 cut(s)	133, 362, 676, 1907, 2941, 3175
BseYI	CCCAGC	1 cut(s)	2589
BsgI	GTGCAG	1 cut(s)	3304
Bsh1236I	CGCG	7 cut(s)	8, 13, 152, 203, 212, 1982, 2246
Bsh1285I	CGRYCG	2 cut(s)	171, 2244
BshFI	GGCC	8 cut(s)	5, 86, 1265, 1421, 2678, 2814, 3205, 3271
BshNI	GGYRCC	1 cut(s)	2821
BshVI	ATCGAT	1 cut(s)	996
BsiEI	CGRYCG	2 cut(s)	171, 2244
BsiSI	CCGG	3 cut(s)	87, 2468, 3233
BslFI	GGGAC	8 cut(s)	129, 720, 938, 1385, 1555, 2607, 2924, 3221
BslI	CCNNNNNNNGG	8 cut(s)	36, 79, 93, 962, 1260, 1700, 2764, 3076
BsmAI	GTCTC	2 cut(s)	116, 2211
BsmFI	GGGAC	8 cut(s)	129, 720, 938, 1385, 1555, 2607, 2924, 3221
BsmI	GAATGC	5 cut(s)	541, 1754, 2505, 2916, 2984
BsnI	GGCC	8 cut(s)	5, 86, 1265, 1421, 2678, 2814, 3205, 3271
Bso31I	GGTCTC	1 cut(s)	116
Bsp1286I	GDGCHC	2 cut(s)	2758, 2824
Bsp68I	TCGCGA	1 cut(s)	1982
BspANI	GGCC	8 cut(s)	5, 86, 1265, 1421, 2678, 2814, 3205, 3271
BspCNI	CTCAG	9 cut(s)	231, 715, 864, 948, 1166, 1396, 1838, 1949, 2278
BspDI	ATCGAT	1 cut(s)	996
BspFNI	CGCG	7 cut(s)	8, 13, 152, 203, 212, 1982, 2246
BspHI	TCATGA	1 cut(s)	2476
BspLI	GGNNCC	6 cut(s)	68, 2142, 2755, 2823, 2852, 3237
BspMAI	CTGCAG	2 cut(s)	694, 2931
BspOI	GCTAGC	1 cut(s)	1439
BspPI	GGATC	8 cut(s)	167, 321, 604, 1047, 1124, 1729, 2135, 2148
BspQI	GCTCTTC	1 cut(s)	2067
BspT107I	GGYRCC	1 cut(s)	2821
BspTNI	GGTCTC	1 cut(s)	116
BsrBI	CCGCTC	2 cut(s)	64, 2657
BsrDI	GCAATG	6 cut(s)	183, 353, 1687, 2028, 2411, 3173
BssECI	CCNNGG	5 cut(s)	87, 150, 465, 611, 3127
BssNI	GRCGYC	1 cut(s)	50
Bst2UI	CCWGG	2 cut(s)	466, 2154
Bst6I	CTCTTC	4 cut(s)	825, 2067, 2692, 3077
BstACI	GRCGYC	1 cut(s)	50
BstAPI	GCANNNNNTGC	1 cut(s)	2413
BstDSI	CCRYGG	1 cut(s)	150
BstF5I	GGATG	6 cut(s)	324, 746, 1627, 2455, 2560, 2941
BstFNI	CGCG	7 cut(s)	8, 13, 152, 203, 212, 1982, 2246
BstHHI	GCGC	3 cut(s)	205, 409, 1666
BstMAI	GTCTC	2 cut(s)	116, 2211
BstMCI	CGRYCG	2 cut(s)	171, 2244
BstMWI	GCNNNNNNNGC	6 cut(s)	152, 209, 1469, 2413, 3169, 3277
BstNI	CCWGG	2 cut(s)	466, 2154
BstNSI	RCATGY	2 cut(s)	166, 2951
BstSCI	CCNGG	3 cut(s)	86, 464, 2152
BstSFI	CTRYAG	4 cut(s)	690, 1797, 2781, 2927
BstSLI	GKGCMC	1 cut(s)	2824
BstUI	CGCG	7 cut(s)	8, 13, 152, 203, 212, 1982, 2246
BstV1I	GCAGC	6 cut(s)	133, 362, 676, 1907, 2941, 3175
BstV2I	GAAGAC	3 cut(s)	18, 1804, 3037
BstX2I	RGATCY	6 cut(s)	326, 894, 1291, 1721, 2140, 2375
BstYI	RGATCY	6 cut(s)	326, 894, 1291, 1721, 2140, 2375
Bsu15I	ATCGAT	1 cut(s)	996
Bsu36I	CCTNAGG	1 cut(s)	1404
BsuI	GTATCC	2 cut(s)	1153, 2737
BsuRI	GGCC	8 cut(s)	5, 86, 1265, 1421, 2678, 2814, 3205, 3271
BsuTUI	ATCGAT	1 cut(s)	996
BtgI	CCRYGG	1 cut(s)	150
BtsCI	GGATG	6 cut(s)	324, 746, 1627, 2455, 2560, 2941
BtsI	GCAGTG	2 cut(s)	1534, 2175
BtsIMutI	CAGTG	9 cut(s)	427, 975, 1275, 1534, 1631, 1674, 2175, 2211, 3048
BtuMI	TCGCGA	1 cut(s)	1982
CaiI	CAGNNNCTG	4 cut(s)	1238, 1403, 2189, 2722
CciI	TCATGA	1 cut(s)	2476
CfoI	GCGC	3 cut(s)	205, 409, 1666
Cfr13I	GGNCC	4 cut(s)	868, 1263, 1477, 3235
Cfr42I	CCGCGG	1 cut(s)	153
ClaI	ATCGAT	1 cut(s)	996
CseI	GACGC	4 cut(s)	152, 223, 1441, 2556
Csp6I	GTAC	8 cut(s)	271, 929, 1092, 1927, 2201, 2714, 3101, 3259
CviQI	GTAC	8 cut(s)	271, 929, 1092, 1927, 2201, 2714, 3101, 3259
DraI	TTTAAA	2 cut(s)	1554, 2706
DrdI	GACNNNNNNGTC	1 cut(s)	55
DseDI	GACNNNNNNGTC	1 cut(s)	55
EaeI	YGGCCR	4 cut(s)	3, 1419, 2676, 2812
Eam1104I	CTCTTC	4 cut(s)	825, 2067, 2692, 3077
EarI	CTCTTC	4 cut(s)	825, 2067, 2692, 3077
EciI	GGCGGA	2 cut(s)	331, 640
Eco24I	GRGCYC	1 cut(s)	2758
Eco31I	GGTCTC	1 cut(s)	116
Eco32I	GATATC	1 cut(s)	2542
Eco47I	GGWCC	3 cut(s)	868, 1477, 3235
Eco57I	CTGAAG	5 cut(s)	806, 2538, 3051, 3089, 3247
Eco81I	CCTNAGG	1 cut(s)	1404
EcoO109I	RGGNCCY	1 cut(s)	868
EcoRII	CCWGG	2 cut(s)	464, 2152
EcoRV	GATATC	1 cut(s)	2542
EcoT22I	ATGCAT	2 cut(s)	2799, 2986
EcoT38I	GRGCYC	1 cut(s)	2758
FalI	AAGNNNNNCTT	2 cut(s)	2094, 2126
FaqI	GGGAC	8 cut(s)	129, 720, 938, 1385, 1555, 2607, 2924, 3221
FauI	CCCGC	1 cut(s)	217
FauNDI	CATATG	3 cut(s)	1579, 2044, 3176
FbaI	TGATCA	3 cut(s)	681, 946, 2736
FblI	GTMKAC	1 cut(s)	1796
FokI	GGATG	6 cut(s)	331, 753, 1614, 2462, 2567, 2948
FriOI	GRGCYC	1 cut(s)	2758
FspBI	CTAG	7 cut(s)	242, 443, 1005, 1295, 1329, 1436, 2750
FspI	TGCGCA	1 cut(s)	1665
GlaI	GCGC	3 cut(s)	204, 408, 1665
GsaI	CCCAGC	1 cut(s)	2593
GsuI	CTGGAG	5 cut(s)	140, 752, 884, 2235, 3072
HaeIII	GGCC	8 cut(s)	5, 86, 1265, 1421, 2678, 2814, 3205, 3271
HapII	CCGG	3 cut(s)	87, 2468, 3233
HgaI	GACGC	4 cut(s)	152, 223, 1441, 2556
HhaI	GCGC	3 cut(s)	205, 409, 1666
Hin1I	GRCGYC	1 cut(s)	50
Hin6I	GCGC	3 cut(s)	203, 407, 1664
HinP1I	GCGC	3 cut(s)	203, 407, 1664
HincII	GTYRAC	1 cut(s)	2731
HindII	GTYRAC	1 cut(s)	2731
HindIII	AAGCTT	2 cut(s)	651, 1341
HinfI	GANTC	9 cut(s)	639, 708, 966, 1001, 1613, 1793, 1865, 1934, 3244
HpaI	GTTAAC	1 cut(s)	2731
HpaII	CCGG	3 cut(s)	87, 2468, 3233
HphI	GGTGA	8 cut(s)	437, 932, 956, 1028, 1178, 1372, 1715, 2438
Hpy166II	GTNNAC	4 cut(s)	273, 1033, 1797, 2731
Hpy8I	GTNNAC	4 cut(s)	273, 1033, 1797, 2731
Hpy99I	CGWCG	8 cut(s)	13, 25, 52, 55, 140, 143, 146, 217
HpyAV	CCTTC	7 cut(s)	87, 187, 538, 731, 1814, 2297, 2811
HpyCH4IV	ACGT	2 cut(s)	50, 2596
HpyF10VI	GCNNNNNNNGC	6 cut(s)	152, 209, 1469, 2413, 3169, 3277
HpySE526I	ACGT	2 cut(s)	50, 2596
Hsp92I	GRCGYC	1 cut(s)	50
HspAI	GCGC	3 cut(s)	203, 407, 1664
Ksp22I	TGATCA	3 cut(s)	681, 946, 2736
KspAI	GTTAAC	1 cut(s)	2731
KspI	CCGCGG	1 cut(s)	153
LguI	GCTCTTC	1 cut(s)	2067
LmnI	GCTCC	5 cut(s)	61, 1352, 1892, 2470, 2753
Lsp1109I	GCAGC	6 cut(s)	133, 362, 676, 1907, 2941, 3175
LweI	GCATC	5 cut(s)	1158, 1636, 2155, 2269, 2452
MaeI	CTAG	7 cut(s)	242, 443, 1005, 1295, 1329, 1436, 2750
MaeII	ACGT	2 cut(s)	50, 2596
MbiI	CCGCTC	2 cut(s)	64, 2657
MflI	RGATCY	6 cut(s)	326, 894, 1291, 1721, 2140, 2375
MhlI	GDGCHC	2 cut(s)	2758, 2824
MlyI	GAGTC	5 cut(s)	702, 1802, 1859, 1928, 3238
MmeI	TCCRAC	2 cut(s)	39, 2659
Mph1103I	ATGCAT	2 cut(s)	2799, 2986
MslI	CAYNNNNRTG	3 cut(s)	1385, 3053, 3119
MspA1I	CMGCKG	4 cut(s)	152, 797, 1898, 3280
MspI	CCGG	3 cut(s)	87, 2468, 3233
MspR9I	CCNGG	3 cut(s)	88, 466, 2154
Mva1269I	GAATGC	5 cut(s)	541, 1754, 2505, 2916, 2984
MvaI	CCWGG	2 cut(s)	466, 2154
MvnI	CGCG	7 cut(s)	8, 13, 152, 203, 212, 1982, 2246
MwoI	GCNNNNNNNGC	6 cut(s)	152, 209, 1469, 2413, 3169, 3277
NciI	CCSGG	1 cut(s)	88
NdeI	CATATG	3 cut(s)	1579, 2044, 3176
NheI	GCTAGC	1 cut(s)	1435
NlaIV	GGNNCC	6 cut(s)	68, 2142, 2755, 2823, 2852, 3237
NmuCI	GTSAC	4 cut(s)	973, 1378, 2209, 3055
NruI	TCGCGA	1 cut(s)	1982
NsbI	TGCGCA	1 cut(s)	1665
NsiI	ATGCAT	2 cut(s)	2799, 2986
NspI	RCATGY	2 cut(s)	166, 2951
OliI	CACNNNNGTG	2 cut(s)	1385, 3053
PaeI	GCATGC	1 cut(s)	2951
PagI	TCATGA	1 cut(s)	2476
PciSI	GCTCTTC	1 cut(s)	2067
PcsI	WCGNNNNNNNCGW	3 cut(s)	17, 29, 2593
PctI	GAATGC	5 cut(s)	541, 1754, 2505, 2916, 2984
PfeI	GAWTC	4 cut(s)	639, 966, 1001, 1613
PflMI	CCANNNNNTGG	2 cut(s)	79, 1700
Ple19I	CGATCG	1 cut(s)	2244
PleI	GAGTC	5 cut(s)	702, 1801, 1859, 1928, 3238
PpsI	GAGTC	5 cut(s)	702, 1801, 1859, 1928, 3238
PpuMI	RGGWCCY	1 cut(s)	868
PshBI	ATTAAT	2 cut(s)	1992, 2667
PsiI	TTATAA	3 cut(s)	674, 785, 2831
Psp5II	RGGWCCY	1 cut(s)	868
Psp6I	CCWGG	2 cut(s)	464, 2152
PspFI	CCCAGC	1 cut(s)	2589
PspGI	CCWGG	2 cut(s)	464, 2152
PspN4I	GGNNCC	6 cut(s)	68, 2142, 2755, 2823, 2852, 3237
PspPI	GGNCC	4 cut(s)	868, 1263, 1477, 3235
PspPPI	RGGWCCY	1 cut(s)	868
PsrI	GAACNNNNNNTAC	2 cut(s)	2568, 2600
PstI	CTGCAG	2 cut(s)	694, 2931
PstNI	CAGNNNCTG	4 cut(s)	1238, 1403, 2189, 2722
PsuI	RGATCY	6 cut(s)	326, 894, 1291, 1721, 2140, 2375
PvuI	CGATCG	1 cut(s)	2244
PvuII	CAGCTG	1 cut(s)	797
RgaI	GCGATCGC	1 cut(s)	2244
RruI	TCGCGA	1 cut(s)	1982
RsaI	GTAC	8 cut(s)	272, 930, 1093, 1928, 2202, 2715, 3102, 3260
RsaNI	GTAC	8 cut(s)	271, 929, 1092, 1927, 2201, 2714, 3101, 3259
RseI	CAYNNNNRTG	3 cut(s)	1385, 3053, 3119
SacII	CCGCGG	1 cut(s)	153
SapI	GCTCTTC	1 cut(s)	2067
Sau96I	GGNCC	4 cut(s)	868, 1263, 1477, 3235
ScaI	AGTACT	1 cut(s)	3260
SchI	GAGTC	5 cut(s)	702, 1802, 1859, 1928, 3238
ScrFI	CCNGG	3 cut(s)	88, 466, 2154
SduI	GDGCHC	2 cut(s)	2758, 2824
SfaAI	GCGATCGC	1 cut(s)	2244
SfaNI	GCATC	5 cut(s)	1158, 1636, 2155, 2269, 2452
SfcI	CTRYAG	4 cut(s)	690, 1797, 2781, 2927
Sfr303I	CCGCGG	1 cut(s)	153
SgfI	GCGATCGC	1 cut(s)	2244
SgrBI	CCGCGG	1 cut(s)	153
SinI	GGWCC	3 cut(s)	868, 1477, 3235
SmiMI	CAYNNNNRTG	3 cut(s)	1385, 3053, 3119
SmlI	CTYRAG	1 cut(s)	634
SmoI	CTYRAG	1 cut(s)	634
SpeI	ACTAGT	1 cut(s)	241
SphI	GCATGC	1 cut(s)	2951
SspI	AATATT	3 cut(s)	903, 1957, 2614
SspMI	CTAG	7 cut(s)	242, 443, 1005, 1295, 1329, 1436, 2750
StyD4I	CCNGG	3 cut(s)	86, 464, 2152
TaiI	ACGT	2 cut(s)	53, 2599
TaqI	TCGA	4 cut(s)	180, 533, 996, 3022
TaqII	GACCGA	2 cut(s)	140, 185
TatI	WGTACW	3 cut(s)	2713, 3100, 3258
TauI	GCSGC	6 cut(s)	8, 152, 155, 239, 2657, 2681
TfiI	GAWTC	4 cut(s)	639, 966, 1001, 1613
TscAI	CASTG	9 cut(s)	427, 982, 1282, 1534, 1638, 1681, 2175, 2218, 3055
TseFI	GTSAC	4 cut(s)	973, 1378, 2209, 3055
TseI	GCWGC	6 cut(s)	146, 350, 689, 1895, 2929, 3163
Tsp45I	GTSAC	4 cut(s)	973, 1378, 2209, 3055
TspDTI	ATGAA	8 cut(s)	1014, 1463, 1784, 1796, 1865, 2085, 2465, 3064
TspGWI	ACGGA	4 cut(s)	1469, 1745, 2306, 3237
TspRI	CASTG	9 cut(s)	427, 982, 1282, 1534, 1638, 1681, 2175, 2218, 3055
Van91I	CCANNNNNTGG	2 cut(s)	79, 1700
VpaK11BI	GGWCC	3 cut(s)	868, 1477, 3235
VspI	ATTAAT	2 cut(s)	1992, 2667
XapI	RAATTY	6 cut(s)	1362, 1973, 2621, 3000, 3134, 3289
XbaI	TCTAGA	1 cut(s)	1294
XceI	RCATGY	2 cut(s)	166, 2951
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1316
XmiI	GTMKAC	1 cut(s)	1796
XspI	CTAG	7 cut(s)	242, 443, 1005, 1295, 1329, 1436, 2750
ZraI	GACGTC	1 cut(s)	51
ZrmI	AGTACT	1 cut(s)	3260
Zsp2I	ATGCAT	2 cut(s)	2799, 2986

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.