MD09G1173200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012135
Physical Location & Seq
Forward (+)
14675627 .. 14676845
1219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1173200.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGCTGCAGCAACAGCATGTTGCTTTTCTCCTGCTAATTCTCTCTCCATCCAAAAATCTGGGCCAGTTTTAAAGCCTTTCAGCAGAGTACTGAGGCTGAAAAGCAGTCAACTAACAGTGAAGATCAAAAGCTCAACCCTCCAGATCAGATCCTCCTTGAGAGAAGAGGTTTTTGAGGATCGGTCCAATGGAATAATATGCTACAAGGATGACAGAGGGGAGATAATTTGTGAAGGGTTTGATGAGGGTCCTAGGTATCACCAACATAATCCAAGAACACCTTGGCAACCCAGAGACACAGAGATCTTTGATCATCTTCAACAGAGGTGGATTAATTTCATAACAGGCACAGAATTGAGCCGTGGCGACAAAGGCAGTGTTTTTCTGCAAGAGGACATAAAAAACTGCAATGGCTTCAACACTTTCCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

144

Amino Acids

16.35

Weight (kDa)

8.66

Isoelectric Point (pI)

51.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012135)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14280
fragaria_vesca FvH4_6g35780
malus_domestica MD09G1173200.v1.1 MD17G1154100.v1.1
prunus_persica Prupe.3G031300_v2.0.a1 Prupe.3G031300_v2.0.a1
pyrus_communis pycom09g09060 pycom17g14680
rosa_chinensis RchiOBHm_Chr2g0147821
rosa_laevigata RLG00000020281
rosa_multiflora Rmu_sc0000308.1_g000047
rosa_roxburghii Rroxscaffold_2G00099320
rosa_rugosa Rorug02G0402000 Rorug02G0402100
rosa_samantha Rh2AG459100 Rh2BG472400 Rh2CG446500 Rh2DG481400
rosa_wichuraiana Rw2G037550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 427
AclWI GGATC 2 cut(s) 144, 186
AfaI GTAC 1 cut(s) 90
AgsI TTSAA 2 cut(s) 320, 418
AluBI AGCT 1 cut(s) 132
AluI AGCT 1 cut(s) 132
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 2 cut(s) 144, 186
AoxI GGCC 1 cut(s) 62
ApeKI GCWGC 2 cut(s) 5, 8
AseI ATTAAT 1 cut(s) 333
AspA2I CCTAGG 1 cut(s) 251
AspS9I GGNCC 3 cut(s) 62, 183, 248
AsuHPI GGTGA 1 cut(s) 251
AvaII GGWCC 2 cut(s) 183, 248
AvrII CCTAGG 1 cut(s) 251
BbvI GCAGC 1 cut(s) 20
BccI CCATC 1 cut(s) 56
BceAI ACGGC 1 cut(s) 345
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 1 cut(s) 6
BglII AGATCT 1 cut(s) 303
BisI GCNGC 2 cut(s) 6, 9
BlnI CCTAGG 1 cut(s) 251
BlsI GCNGC 2 cut(s) 7, 10
BmcAI AGTACT 1 cut(s) 90
Bme18I GGWCC 2 cut(s) 183, 248
BmgT120I GGNCC 3 cut(s) 62, 183, 248
BmiI GGNNCC 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 178
BsaJI CCNNGG 3 cut(s) 251, 281, 361
Bse1I ACTGG 1 cut(s) 65
Bse3DI GCAATG 1 cut(s) 415
BseDI CCNNGG 3 cut(s) 251, 281, 361
BseGI GGATG 2 cut(s) 48, 214
BseMI GCAATG 1 cut(s) 415
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 1 cut(s) 65
BseXI GCAGC 1 cut(s) 20
BshFI GGCC 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 288
BsnI GGCC 1 cut(s) 64
Bsp143I GATC 6 cut(s) 123, 144, 149, 178, 303, 310
BspACI CCGC 1 cut(s) 427
BspANI GGCC 1 cut(s) 64
BspCNI CTCAG 1 cut(s) 84
BspLI GGNNCC 1 cut(s) 249
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 2 cut(s) 144, 186
BsrDI GCAATG 1 cut(s) 415
BsrI ACTGG 1 cut(s) 65
BssECI CCNNGG 3 cut(s) 251, 281, 361
BssMI GATC 6 cut(s) 123, 144, 149, 178, 303, 310
BssT1I CCWWGG 2 cut(s) 251, 281
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 1 cut(s) 159
BstDEI CTNAG 1 cut(s) 92
BstDSI CCRYGG 1 cut(s) 361
BstF5I GGATG 2 cut(s) 48, 214
BstKTI GATC 6 cut(s) 126, 147, 152, 181, 306, 313
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 6 cut(s) 123, 144, 149, 178, 303, 310
BstMWI GCNNNNNNNGC 2 cut(s) 14, 372
BstNSI RCATGY 1 cut(s) 21
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 1 cut(s) 20
BstX2I RGATCY 2 cut(s) 149, 303
BstXI CCANNNNNNTGG 1 cut(s) 59
BstYI RGATCY 2 cut(s) 149, 303
BsuRI GGCC 1 cut(s) 64
BtgI CCRYGG 1 cut(s) 361
BtsCI GGATG 2 cut(s) 48, 214
BtsI GCAGTG 1 cut(s) 382
BtsIMutI CAGTG 2 cut(s) 123, 382
Cfr13I GGNCC 3 cut(s) 62, 183, 248
Csp6I GTAC 1 cut(s) 89
CspCI CAANNNNNGTGG 2 cut(s) 308, 343
CviAII CATG 1 cut(s) 18
CviJI RGCY 7 cut(s) 5, 64, 76, 97, 132, 360, 414
CviKI_1 RGCY 7 cut(s) 5, 64, 76, 97, 132, 360, 414
CviQI GTAC 1 cut(s) 89
DdeI CTNAG 1 cut(s) 92
DpnI GATC 6 cut(s) 125, 146, 151, 180, 305, 312
DpnII GATC 6 cut(s) 123, 144, 149, 178, 303, 310
DraI TTTAAA 1 cut(s) 72
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco130I CCWWGG 2 cut(s) 251, 281
Eco47I GGWCC 2 cut(s) 183, 248
EcoO109I RGGNCCY 1 cut(s) 248
EcoT14I CCWWGG 2 cut(s) 251, 281
ErhI CCWWGG 2 cut(s) 251, 281
FaeI CATG 1 cut(s) 21
FaiI YATR 5 cut(s) 19, 199, 267, 341, 398
FalI AAGNNNNNCTT 2 cut(s) 265, 297
FatI CATG 1 cut(s) 17
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 2 cut(s) 6, 9
FokI GGATG 2 cut(s) 35, 221
Fsp4HI GCNGC 2 cut(s) 6, 9
FspBI CTAG 1 cut(s) 252
GluI GCNGC 2 cut(s) 6, 9
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 1 cut(s) 64
Hin1II CATG 1 cut(s) 21
HincII GTYRAC 1 cut(s) 110
HindII GTYRAC 1 cut(s) 110
HphI GGTGA 1 cut(s) 251
Hpy166II GTNNAC 1 cut(s) 110
Hpy188I TCNGA 1 cut(s) 149
Hpy188III TCNNGA 1 cut(s) 142
Hpy8I GTNNAC 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 227
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4V TGCA 3 cut(s) 8, 388, 408
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 372
HpyF3I CTNAG 1 cut(s) 92
Hsp92II CATG 1 cut(s) 21
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 6 cut(s) 123, 144, 149, 178, 303, 310
LpnPI CCDG 6 cut(s) 45, 45, 78, 155, 304, 330
Lsp1109I GCAGC 1 cut(s) 20
MaeI CTAG 1 cut(s) 252
MalI GATC 6 cut(s) 125, 146, 151, 180, 305, 312
MboI GATC 6 cut(s) 123, 144, 149, 178, 303, 310
MboII GAAGA 3 cut(s) 133, 176, 308
MflI RGATCY 2 cut(s) 149, 303
MluCI AATT 4 cut(s) 37, 225, 334, 353
MnlI CCTC 9 cut(s) 87, 149, 160, 163, 169, 209, 238, 318, 385
MseI TTAA 2 cut(s) 71, 333
MwoI GCNNNNNNNGC 2 cut(s) 14, 372
NdeII GATC 6 cut(s) 123, 144, 149, 178, 303, 310
NlaIII CATG 1 cut(s) 21
NlaIV GGNNCC 1 cut(s) 249
NspI RCATGY 1 cut(s) 21
PkrI GCNGC 2 cut(s) 7, 10
PpuMI RGGWCCY 1 cut(s) 248
PshBI ATTAAT 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 248
PspN4I GGNNCC 1 cut(s) 249
PspPI GGNCC 3 cut(s) 62, 183, 248
PspPPI RGGWCCY 1 cut(s) 248
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 2 cut(s) 149, 303
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
SaqAI TTAA 2 cut(s) 71, 333
SatI GCNGC 2 cut(s) 6, 9
Sau3AI GATC 6 cut(s) 123, 144, 149, 178, 303, 310
Sau96I GGNCC 3 cut(s) 62, 183, 248
ScaI AGTACT 1 cut(s) 90
SetI ASST 5 cut(s) 134, 171, 257, 283, 329
SfcI CTRYAG 1 cut(s) 6
SinI GGWCC 2 cut(s) 183, 248
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
Sse9I AATT 4 cut(s) 37, 225, 334, 353
SsiI CCGC 1 cut(s) 427
SspMI CTAG 1 cut(s) 252
StyI CCWWGG 2 cut(s) 251, 281
TaaI ACNGT 1 cut(s) 118
TaqII GACCGA 1 cut(s) 171
TasI AATT 4 cut(s) 37, 225, 334, 353
TatI WGTACW 1 cut(s) 88
Tru1I TTAA 2 cut(s) 71, 333
Tru9I TTAA 2 cut(s) 71, 333
TscAI CASTG 2 cut(s) 123, 382
TseI GCWGC 2 cut(s) 5, 8
TspDTI ATGAA 1 cut(s) 328
TspRI CASTG 2 cut(s) 123, 382
VpaK11BI GGWCC 2 cut(s) 183, 248
VspI ATTAAT 1 cut(s) 333
XceI RCATGY 1 cut(s) 21
XcmI CCANNNNNNNNNTGG 1 cut(s) 279
XmaJI CCTAGG 1 cut(s) 251
XspI CTAG 1 cut(s) 252
ZrmI AGTACT 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.