MD09G1181600.v1.1

guanosine tetraphosphate biosynthetic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011437
Physical Location & Seq
Forward (+)
15637485 .. 15641972
4488 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1181600.v1.1.491

Sequence Viewer

Length: 2238 bp
ATGACGGTCCCCACAATAGCTCTGTACGCGAGCCCGCCGAGCAGTGTGTGCTCCACGGCGCACCCGTGCCAGATCAACGCGCACGCCTCACACGATTTCGAATTGAGCTCTCGATCTTCGTCGTCGACGGCGTCGACGCCCTCCGCGTCGCAGAAGCCGGTGGCTGGGGGGCTGTCGTGCCTGTTCTCGTCGCAGACGGTGAAGCACGCGTCATCGTCGTCGTCCAGCTTTTCGGGTGGAGGAGAGGAATTGGGTTCTCGGTGGCACGATAGAGGCGAGGAATTGAAGGAATTGAGCAGCTCGTTTCGGTATTCGGGGAGTAAGTTCAATGGGGCTTCGTTGAACCGGGACCAGAGCCCGGTTTCGGTGTTTCAGGGTCCGGTCTCGTCTTCCAGTAGCGGGGTTTCGGGGTCGGCGAGAAGCCCGCCGATGAGAATTGCAAGGGAAAGGTCTAGCAATGGAGATATTAGCTTGAATTCTATTCGGTGTGGGAGTAATGGGTTGTTTAATGGGTTTGTGAGGGGTGCTTTGGGCTCTTCCTGCATAGATTACGCCTCGGCGAGCTTCGAGGCTCAGACTGATGGTTTGGATGTGGGTTCTTCAGCTGTGCTTGATGAATTGACTTTCAACATGGAAGATGGGTTTTTGGAGGGAATTGCTGAGCCTTATGCTAAGGAGTTGCTAGTGGGTGCGCAGCTGCGGCACAAGATTTTCTATGAAGATTTTATTATCAAGGCCTTTTACGAGGCCGAAAAAGCGCATAGAGGGCAGATGCGAGCAAGTGGCGATCCTTATTTGCAGCATTGTGTGGAGACGGCGGTGTTGCTCGCATTGATCGGTGCTAATTCCACAGTTGTTGCTGCAGGGCTTTTACATGACACGCTTGATGATTCTTTTATGTGTTATGACTACATATTTGGGAAATTTGGAGCTGGGGTTGCTGATTTAGTAGAAGGGGTGTCTAAGCTCAGTCATTTGAGCAAGCTTGCCCGTGATAATAATACAGCATGCAAAACAGTTGAGGCAGATCGCCTGCATACCATGTTCCTTGCCATGGCAGATGCCCGTGCTGTCCTCATTAAATTGGCAGACCGATTGCATAATATGATTACACTAGATGCATTACCCTTGGCTAAACAACAGAGGTTTGCAAAGGAGACTTTGGAGATTTTTGTGCCCTTGGCCAACAGACTTGGAATCTCTAGCTGGAAGGTGCAGCTGGAAAATTTATGTTTTAAGCATCTCAACCCGGATCAGCACAAAGAACTGTCCTCTAAGCTTGTAGATTCGTTTGACGAGGCAATGATTATTTCTGCCACGGAGACATTAGAGCGTGCTCTCAAGGATAAAGCCATTTCCTACCATGTTCTTTGTGGGCGGCATAAGAGCTTGTATAGCATCTACTCTAAAATGTTAAAGAAGAAGCTAAACATGGATGAAATACATGATATTCATGGGCTGCGTTTGATTGTCGAAAATGAGGATGATTGTTACGAGGCACTTAAAGTTGTTCACCAGTTGTGGTCTGAGGTCCCAGGGAAGTTCAAGGACTACATAACTCAACCCAAGTGTAATGGGTATCAGTCATTGCACACTGTGGTGATGGGTGAAGGCGTGATTCCACTGGAAGTCCAAATTCGAACAAAGGAGATGCATTTGCAGGCTGAGTTTGGATTTGCAGCTCATTGGAGATACAAGGAAGGTGACTGTAAGCACCCCTCATTTGTGCTTCAAATGGTTGAGTGGGCCAGATGGGTGGTCACTTGGCAGTGCGAGGCAATGAACAGAGACAGGTCCTCCGTTGGCTGTGACGATTCAATCAAGCCGCCATGCACGTTTCCTACCCATTCTGATGACTGTCCATATTCTTACAAGCCCCACTGTGGTCAAGACGGGCCGGTGTTTGTCATCATGCTAGAGAATGATAAGATGTCTGTGCAAGAGTATCCTGCAAACTCCACTGTTATGGATCTGCTGGAAAAAGCTGGGCGAGGGAGCTTGAGATGGACTCCGTACGGGTTCCCACTGAAGGAAGAACTGAGGCCGAGGCTGAACCATGCGCCAGTGAACGATCCTACATGCAAGCTGCAGATGGGGGATGTGGTGGAGCTGACTCCAGCTATTCCCGATAAGTCTTTGACAGAGTACAGAGAAGAGATTCAGCGCATGTACGACCGGGGCACAAGTGTATCGAGTGCAGGGCCTCCTGCTAACCGTATGGCGGGTTGGAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008728 GO:0008893 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009150 GO:0009152 GO:0009163 GO:0009165 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0009991 GO:0010033 GO:0010150 GO:0015969 GO:0015970 GO:0015979 GO:0016020 GO:0016740 GO:0016772 GO:0016778 GO:0016787 GO:0016788 GO:0016794 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0030312 GO:0031667 GO:0032501 GO:0032502 GO:0033865 GO:0033866 GO:0033875 GO:0033993 GO:0034030 GO:0034032 GO:0034033 GO:0034035 GO:0034036 GO:0034404 GO:0034641 GO:0034654 GO:0042221 GO:0042278 GO:0042451 GO:0042455 GO:0042578 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046128 GO:0046129 GO:0046390 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0090693 GO:0097305 GO:0099402 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00230 map00230
Pfam Domains
Protein Families
HD_4 RelA_SpoT

Protein Analysis

746

Amino Acids

82.2

Weight (kDa)

6.07

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD_4 PF13328 246 - 400 5.3e-39 HD domain
HD PF01966 265 - 369 3.6e-08 HD domain
RelA_SpoT PF04607 459 - 568 3e-42 Region found in RelA / SpoT proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14050
fragaria_vesca FvH4_6g34880 FvH4_6g34880 FvH4_6g34880
malus_domestica MD09G1181600.v1.1 MD17G1168000.v1.1
prunus_persica Prupe.3G039700_v2.0.a1
pyrus_communis pycom09g09930 pycom17g15960
rosa_chinensis RchiOBHm_Chr2g0145751
rosa_laevigata RLG00000010129 RLG00000020166
rosa_multiflora Rmu_sc0000473.1_g000007
rosa_roxburghii Rroxscaffold_152G00434730 Rroxscaffold_2G00100470
rosa_rugosa Rorug02G0393100
rosa_samantha Rh2AG447400 Rh2BG459700 Rh2CG434200
rosa_wichuraiana Rw2G036560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 693
AccI GTMKAC 2 cut(s) 125, 134
AccII CGCG 4 cut(s) 29, 80, 146, 209
AciI CCGC 9 cut(s) 35, 144, 399, 425, 700, 818, 1378, 1826, 2222
AclWI GGATC 4 cut(s) 782, 1260, 1977, 2066
AcoI YGGCCR 1 cut(s) 1182
AcsI RAATTY 4 cut(s) 475, 923, 1225, 1635
AcuI CTGAAG 2 cut(s) 585, 2048
AcyI GRCGYC 2 cut(s) 131, 137
AdeI CACNNNGTG 1 cut(s) 1597
AfaI GTAC 4 cut(s) 26, 2015, 2147, 2171
AfiI CCNNNNNNNGG 8 cut(s) 164, 358, 364, 399, 1054, 1883, 2029, 2221
AflIII ACRYGT 1 cut(s) 207
AgsI TTSAA 8 cut(s) 286, 328, 343, 475, 628, 1546, 1733, 1818
AjnI CCWGG 1 cut(s) 1534
AleI CACNNNNGTG 1 cut(s) 1598
Alw21I GWGCWC 3 cut(s) 53, 110, 1339
Alw26I GTCTC 5 cut(s) 388, 806, 1151, 1316, 1782
AlwI GGATC 4 cut(s) 782, 1260, 1977, 2066
AoxI GGCC 7 cut(s) 735, 747, 1182, 1746, 1895, 2042, 2201
ApeKI GCWGC 9 cut(s) 297, 694, 697, 799, 860, 1216, 1459, 1679, 2086
ApoI RAATTY 4 cut(s) 475, 923, 1225, 1635
ArsI GACNNNNNNTTYG 4 cut(s) 568, 600, 899, 931
Asp700I GAANNNNTTC 1 cut(s) 2157
AspLEI GCGC 6 cut(s) 61, 82, 694, 760, 2062, 2166
AspS9I GGNCC 8 cut(s) 7, 349, 377, 1531, 1746, 1794, 1895, 2201
AsuC2I CCSGG 4 cut(s) 347, 359, 1250, 2177
AsuHPI GGTGA 5 cut(s) 211, 1505, 1612, 1619, 1715
AsuII TTCGAA 2 cut(s) 99, 1639
AvaII GGWCC 5 cut(s) 7, 349, 377, 1531, 1794
BaeGI GKGCMC 2 cut(s) 1179, 2183
BaeI ACNNNNGTAYC 2 cut(s) 2172, 2205
BalI TGGCCA 1 cut(s) 1184
BanII GRGCYC 4 cut(s) 35, 110, 359, 536
BbsI GAAGAC 1 cut(s) 381
Bbv12I GWGCWC 3 cut(s) 53, 110, 1339
BbvI GCAGC 9 cut(s) 309, 684, 706, 811, 847, 1228, 1446, 1691, 2073
BccI CCATC 6 cut(s) 575, 632, 1597, 1746, 1998, 2086
BceAI ACGGC 3 cut(s) 72, 144, 831
BciT130I CCWGG 1 cut(s) 1536
BciVI GTATCC 1 cut(s) 1956
BcnI CCSGG 4 cut(s) 347, 359, 1250, 2177
BcoDI GTCTC 5 cut(s) 388, 806, 1151, 1316, 1782
BfaI CTAG 5 cut(s) 453, 683, 1115, 1203, 1916
BfmI CTRYAG 2 cut(s) 861, 2087
BfuI GTATCC 1 cut(s) 1956
BlpI GCTNAGC 1 cut(s) 660
Bme1390I CCNGG 5 cut(s) 347, 359, 1250, 1536, 2177
Bme18I GGWCC 5 cut(s) 7, 349, 377, 1531, 1794
BmgT120I GGNCC 8 cut(s) 7, 349, 377, 1531, 1746, 1794, 1895, 2201
BmiI GGNNCC 5 cut(s) 9, 350, 378, 1533, 2021
BmrFI CCNGG 5 cut(s) 347, 359, 1250, 1536, 2177
BmsI GCATC 6 cut(s) 762, 1051, 1108, 1249, 1407, 1641
BpiI GAAGAC 1 cut(s) 381
BplI GAGNNNNNCTC 2 cut(s) 1993, 2025
BpmI CTGGAG 1 cut(s) 2100
Bpu10I CCTNAGC 1 cut(s) 672
Bpu1102I GCTNAGC 1 cut(s) 660
Bpu14I TTCGAA 2 cut(s) 99, 1639
BpuEI CTTGAG 2 cut(s) 1325, 2020
BpuMI CCSGG 4 cut(s) 347, 359, 1250, 2177
BsaHI GRCGYC 2 cut(s) 131, 137
BsaI GGTCTC 1 cut(s) 388
BsaWI WCCGGW 1 cut(s) 379
BsaXI ACNNNNNCTCC 4 cut(s) 921, 951, 1781, 1811
Bsc4I CCNNNNNNNGG 8 cut(s) 164, 358, 364, 399, 1054, 1883, 2029, 2221
Bse118I RCCGGY 2 cut(s) 157, 1897
Bse1I ACTGG 4 cut(s) 393, 1516, 1629, 2063
Bse3DI GCAATG 4 cut(s) 463, 1308, 1586, 1785
BseBI CCWGG 1 cut(s) 1536
BseGI GGATG 4 cut(s) 595, 1441, 1489, 2104
BseLI CCNNNNNNNGG 8 cut(s) 164, 358, 364, 399, 1054, 1883, 2029, 2221
BseMI GCAATG 4 cut(s) 463, 1308, 1586, 1785
BseMII CTCAG 6 cut(s) 587, 651, 982, 1518, 1656, 2030
BseNI ACTGG 4 cut(s) 393, 1516, 1629, 2063
BseRI GAGGAG 1 cut(s) 255
BseSI GKGCMC 2 cut(s) 1179, 2183
BseXI GCAGC 9 cut(s) 309, 684, 706, 811, 847, 1228, 1446, 1691, 2073
BseYI CCCAGC 3 cut(s) 164, 932, 1985
BsgI GTGCAG 2 cut(s) 1235, 2217
Bsh1236I CGCG 4 cut(s) 29, 80, 146, 209
Bsh1285I CGRYCG 1 cut(s) 2176
BshFI GGCC 7 cut(s) 737, 749, 1184, 1748, 1897, 2044, 2203
BsiEI CGRYCG 1 cut(s) 2176
BsiHKAI GWGCWC 3 cut(s) 53, 110, 1339
BsiSI CCGG 7 cut(s) 158, 346, 359, 380, 1250, 1898, 2176
BsiWI CGTACG 1 cut(s) 2013
BslFI GGGAC 2 cut(s) 362, 1517
BslI CCNNNNNNNGG 8 cut(s) 164, 358, 364, 399, 1054, 1883, 2029, 2221
BsmAI GTCTC 5 cut(s) 388, 806, 1151, 1316, 1782
BsmBI CGTCTC 1 cut(s) 806
BsmFI GGGAC 2 cut(s) 362, 1517
BsnI GGCC 7 cut(s) 737, 749, 1184, 1748, 1897, 2044, 2203
Bso31I GGTCTC 1 cut(s) 388
Bsp119I TTCGAA 2 cut(s) 99, 1639
Bsp1286I GDGCHC 8 cut(s) 35, 53, 110, 359, 536, 1179, 1339, 2183
Bsp143I GATC 8 cut(s) 72, 113, 787, 834, 1027, 1252, 1969, 2071
Bsp1720I GCTNAGC 1 cut(s) 660
Bsp19I CCATGG 1 cut(s) 1053
BspACI CCGC 9 cut(s) 35, 144, 399, 425, 700, 818, 1378, 1826, 2222
BspANI GGCC 7 cut(s) 737, 749, 1184, 1748, 1897, 2044, 2203
BspCNI CTCAG 6 cut(s) 586, 652, 981, 1519, 1657, 2031
BspFNI CGCG 4 cut(s) 29, 80, 146, 209
BspLI GGNNCC 5 cut(s) 9, 350, 378, 1533, 2021
BspMAI CTGCAG 2 cut(s) 865, 2091
BspPI GGATC 4 cut(s) 782, 1260, 1977, 2066
BspQI GCTCTTC 1 cut(s) 541
BspT104I TTCGAA 2 cut(s) 99, 1639
BspTNI GGTCTC 1 cut(s) 388
BsrDI GCAATG 4 cut(s) 463, 1308, 1586, 1785
BsrFI RCCGGY 2 cut(s) 157, 1897
BsrI ACTGG 4 cut(s) 393, 1516, 1629, 2063
BssAI RCCGGY 2 cut(s) 157, 1897
BssMI GATC 8 cut(s) 72, 113, 787, 834, 1027, 1252, 1969, 2071
BssNI GRCGYC 2 cut(s) 131, 137
BssT1I CCWWGG 3 cut(s) 1053, 1128, 1179
Bst2UI CCWGG 1 cut(s) 1536
Bst6I CTCTTC 2 cut(s) 541, 2148
BstACI GRCGYC 2 cut(s) 131, 137
BstAPI GCANNNNNTGC 1 cut(s) 48
BstBI TTCGAA 2 cut(s) 99, 1639
BstDEI CTNAG 9 cut(s) 573, 660, 672, 963, 968, 1275, 1527, 1665, 2039
BstDSI CCRYGG 3 cut(s) 54, 1053, 1317
BstF5I GGATG 4 cut(s) 595, 1441, 1489, 2104
BstFNI CGCG 4 cut(s) 29, 80, 146, 209
BstHHI GCGC 6 cut(s) 61, 82, 694, 760, 2062, 2166
BstKTI GATC 8 cut(s) 75, 116, 790, 837, 1030, 1255, 1972, 2074
BstMAI GTCTC 5 cut(s) 388, 806, 1151, 1316, 1782
BstMBI GATC 8 cut(s) 72, 113, 787, 834, 1027, 1252, 1969, 2071
BstMCI CGRYCG 1 cut(s) 2176
BstMWI GCNNNNNNNGC 9 cut(s) 26, 39, 48, 540, 700, 755, 766, 938, 1395
BstNI CCWGG 1 cut(s) 1536
BstNSI RCATGY 3 cut(s) 1011, 2082, 2170
BstSCI CCNGG 5 cut(s) 345, 357, 1248, 1534, 2175
BstSFI CTRYAG 2 cut(s) 861, 2087
BstSLI GKGCMC 2 cut(s) 1179, 2183
BstUI CGCG 4 cut(s) 29, 80, 146, 209
BstV1I GCAGC 9 cut(s) 309, 684, 706, 811, 847, 1228, 1446, 1691, 2073
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 1 cut(s) 1969
BstXI CCANNNNNNTGG 2 cut(s) 1756, 1966
BstYI RGATCY 1 cut(s) 1969
BsuI GTATCC 1 cut(s) 1956
BsuRI GGCC 7 cut(s) 737, 749, 1184, 1748, 1897, 2044, 2203
BtgI CCRYGG 3 cut(s) 54, 1053, 1317
BtsCI GGATG 4 cut(s) 595, 1441, 1489, 2104
BtsI GCAGTG 2 cut(s) 49, 1775
BtsIMutI CAGTG 8 cut(s) 49, 1593, 1622, 1775, 1879, 1959, 2024, 2070
CfoI GCGC 6 cut(s) 61, 82, 694, 760, 2062, 2166
Cfr10I RCCGGY 2 cut(s) 157, 1897
Cfr13I GGNCC 8 cut(s) 7, 349, 377, 1531, 1746, 1794, 1895, 2201
CseI GACGC 4 cut(s) 120, 135, 145, 198
Csp6I GTAC 4 cut(s) 25, 2014, 2146, 2170
CspCI CAANNNNNGTGG 2 cut(s) 838, 873
CviQI GTAC 4 cut(s) 25, 2014, 2146, 2170
DdeI CTNAG 9 cut(s) 573, 660, 672, 963, 968, 1275, 1527, 1665, 2039
DpnI GATC 8 cut(s) 74, 115, 789, 836, 1029, 1254, 1971, 2073
DpnII GATC 8 cut(s) 72, 113, 787, 834, 1027, 1252, 1969, 2071
DraIII CACNNNGTG 1 cut(s) 1597
EaeI YGGCCR 1 cut(s) 1182
Eam1104I CTCTTC 2 cut(s) 541, 2148
EarI CTCTTC 2 cut(s) 541, 2148
Ecl136II GAGCTC 1 cut(s) 108
Eco130I CCWWGG 3 cut(s) 1053, 1128, 1179
Eco147I AGGCCT 1 cut(s) 737
Eco24I GRGCYC 4 cut(s) 35, 110, 359, 536
Eco31I GGTCTC 1 cut(s) 388
Eco47I GGWCC 5 cut(s) 7, 349, 377, 1531, 1794
Eco53kI GAGCTC 1 cut(s) 108
Eco57I CTGAAG 2 cut(s) 585, 2048
EcoICRI GAGCTC 1 cut(s) 108
EcoO109I RGGNCCY 3 cut(s) 1531, 1794, 2201
EcoRI GAATTC 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 1534
EcoT14I CCWWGG 3 cut(s) 1053, 1128, 1179
EcoT22I ATGCAT 2 cut(s) 1123, 1656
EcoT38I GRGCYC 4 cut(s) 35, 110, 359, 536
ErhI CCWWGG 3 cut(s) 1053, 1128, 1179
Esp3I CGTCTC 1 cut(s) 806
FaqI GGGAC 2 cut(s) 362, 1517
FauI CCCGC 4 cut(s) 42, 392, 432, 2215
FblI GTMKAC 2 cut(s) 125, 134
FokI GGATG 4 cut(s) 602, 1448, 1496, 2111
FriOI GRGCYC 4 cut(s) 35, 110, 359, 536
FspBI CTAG 5 cut(s) 453, 683, 1115, 1203, 1916
FspI TGCGCA 1 cut(s) 693
GlaI GCGC 6 cut(s) 60, 81, 693, 759, 2061, 2165
GsaI CCCAGC 3 cut(s) 168, 936, 1989
GsuI CTGGAG 1 cut(s) 2100
HaeIII GGCC 7 cut(s) 737, 749, 1184, 1748, 1897, 2044, 2203
HapII CCGG 7 cut(s) 158, 346, 359, 380, 1250, 1898, 2176
HgaI GACGC 4 cut(s) 120, 135, 145, 198
HhaI GCGC 6 cut(s) 61, 82, 694, 760, 2062, 2166
Hin1I GRCGYC 2 cut(s) 131, 137
Hin6I GCGC 6 cut(s) 59, 80, 692, 758, 2060, 2164
HinP1I GCGC 6 cut(s) 59, 80, 692, 758, 2060, 2164
HincII GTYRAC 2 cut(s) 126, 135
HindII GTYRAC 2 cut(s) 126, 135
HindIII AAGCTT 2 cut(s) 983, 1277
HinfI GANTC 8 cut(s) 890, 1197, 1286, 1618, 1814, 2008, 2113, 2158
HpaII CCGG 7 cut(s) 158, 346, 359, 380, 1250, 1898, 2176
HphI GGTGA 5 cut(s) 211, 1505, 1612, 1619, 1715
Hpy166II GTNNAC 4 cut(s) 126, 135, 1513, 2068
Hpy188I TCNGA 3 cut(s) 576, 1528, 1852
Hpy188III TCNNGA 3 cut(s) 111, 1889, 2126
Hpy8I GTNNAC 4 cut(s) 126, 135, 1513, 2068
Hpy99I CGWCG 9 cut(s) 124, 127, 130, 136, 139, 151, 193, 220, 223
HpyAV CCTTC 6 cut(s) 280, 947, 1204, 1604, 1694, 2023
HpyCH4IV ACGT 1 cut(s) 1835
HpyF10VI GCNNNNNNNGC 9 cut(s) 26, 39, 48, 540, 700, 755, 766, 938, 1395
HpyF3I CTNAG 9 cut(s) 573, 660, 672, 963, 968, 1275, 1527, 1665, 2039
HpySE526I ACGT 1 cut(s) 1835
Hsp92I GRCGYC 2 cut(s) 131, 137
HspAI GCGC 6 cut(s) 59, 80, 692, 758, 2060, 2164
Kzo9I GATC 8 cut(s) 72, 113, 787, 834, 1027, 1252, 1969, 2071
LguI GCTCTTC 1 cut(s) 541
LmnI GCTCC 4 cut(s) 56, 929, 1995, 2107
Lsp1109I GCAGC 9 cut(s) 309, 684, 706, 811, 847, 1228, 1446, 1691, 2073
LweI GCATC 6 cut(s) 762, 1051, 1108, 1249, 1407, 1641
MaeI CTAG 5 cut(s) 453, 683, 1115, 1203, 1916
MaeII ACGT 1 cut(s) 1835
MaeIII GTNAC 4 cut(s) 1490, 1703, 1759, 1808
MalI GATC 8 cut(s) 74, 115, 789, 836, 1029, 1254, 1971, 2073
MboI GATC 8 cut(s) 72, 113, 787, 834, 1027, 1252, 1969, 2071
MboII GAAGA 9 cut(s) 108, 381, 528, 591, 647, 731, 1432, 2045, 2165
MflI RGATCY 1 cut(s) 1969
MhlI GDGCHC 8 cut(s) 35, 53, 110, 359, 536, 1179, 1339, 2183
MlsI TGGCCA 1 cut(s) 1184
MluI ACGCGT 1 cut(s) 207
MluNI TGGCCA 1 cut(s) 1184
MlyI GAGTC 2 cut(s) 2002, 2107
MmeI TCCRAC 1 cut(s) 2207
Mox20I TGGCCA 1 cut(s) 1184
Mph1103I ATGCAT 2 cut(s) 1123, 1656
MroXI GAANNNNTTC 1 cut(s) 2157
MscI TGGCCA 1 cut(s) 1184
MseI TTAA 5 cut(s) 507, 1080, 1236, 1415, 1503
MslI CAYNNNNRTG 3 cut(s) 1598, 1851, 1964
Msp20I TGGCCA 1 cut(s) 1184
MspA1I CMGCKG 3 cut(s) 605, 697, 1219
MspI CCGG 7 cut(s) 158, 346, 359, 380, 1250, 1898, 2176
MspR9I CCNGG 5 cut(s) 347, 359, 1250, 1536, 2177
MvaI CCWGG 1 cut(s) 1536
MvnI CGCG 4 cut(s) 29, 80, 146, 209
MwoI GCNNNNNNNGC 9 cut(s) 26, 39, 48, 540, 700, 755, 766, 938, 1395
NciI CCSGG 4 cut(s) 347, 359, 1250, 2177
NcoI CCATGG 1 cut(s) 1053
NdeII GATC 8 cut(s) 72, 113, 787, 834, 1027, 1252, 1969, 2071
NlaIV GGNNCC 5 cut(s) 9, 350, 378, 1533, 2021
NmeAIII GCCGAG 3 cut(s) 63, 536, 2070
NmuCI GTSAC 3 cut(s) 1703, 1759, 1808
NsbI TGCGCA 1 cut(s) 693
NsiI ATGCAT 2 cut(s) 1123, 1656
NspI RCATGY 3 cut(s) 1011, 2082, 2170
NspV TTCGAA 2 cut(s) 99, 1639
OliI CACNNNNGTG 1 cut(s) 1598
PaeI GCATGC 1 cut(s) 1011
PasI CCCWGGG 1 cut(s) 1535
PceI AGGCCT 1 cut(s) 737
PciSI GCTCTTC 1 cut(s) 541
PcsI WCGNNNNNNNCGW 7 cut(s) 62, 90, 128, 131, 143, 273, 413
PdmI GAANNNNTTC 1 cut(s) 2157
PfeI GAWTC 6 cut(s) 890, 1197, 1286, 1618, 1814, 2158
Pfl23II CGTACG 1 cut(s) 2013
PflFI GACNNNGTC 1 cut(s) 130
PleI GAGTC 2 cut(s) 2002, 2107
PpsI GAGTC 2 cut(s) 2002, 2107
PpuMI RGGWCCY 2 cut(s) 1531, 1794
Psp124BI GAGCTC 1 cut(s) 110
Psp5II RGGWCCY 2 cut(s) 1531, 1794
Psp6I CCWGG 1 cut(s) 1534
PspFI CCCAGC 3 cut(s) 164, 932, 1985
PspGI CCWGG 1 cut(s) 1534
PspLI CGTACG 1 cut(s) 2013
PspN4I GGNNCC 5 cut(s) 9, 350, 378, 1533, 2021
PspPI GGNCC 8 cut(s) 7, 349, 377, 1531, 1746, 1794, 1895, 2201
PspPPI RGGWCCY 2 cut(s) 1531, 1794
PstI CTGCAG 2 cut(s) 865, 2091
PsuI RGATCY 1 cut(s) 1969
PsyI GACNNNGTC 1 cut(s) 130
PvuII CAGCTG 3 cut(s) 605, 697, 1219
RsaI GTAC 4 cut(s) 26, 2015, 2147, 2171
RsaNI GTAC 4 cut(s) 25, 2014, 2146, 2170
RseI CAYNNNNRTG 3 cut(s) 1598, 1851, 1964
SacI GAGCTC 1 cut(s) 110
SalI GTCGAC 2 cut(s) 124, 133
SapI GCTCTTC 1 cut(s) 541
SaqAI TTAA 5 cut(s) 507, 1080, 1236, 1415, 1503
Sau3AI GATC 8 cut(s) 72, 113, 787, 834, 1027, 1252, 1969, 2071
Sau96I GGNCC 8 cut(s) 7, 349, 377, 1531, 1746, 1794, 1895, 2201
SchI GAGTC 2 cut(s) 2002, 2107
ScrFI CCNGG 5 cut(s) 347, 359, 1250, 1536, 2177
SduI GDGCHC 8 cut(s) 35, 53, 110, 359, 536, 1179, 1339, 2183
SfaNI GCATC 6 cut(s) 762, 1051, 1108, 1249, 1407, 1641
SfcI CTRYAG 2 cut(s) 861, 2087
SfuI TTCGAA 2 cut(s) 99, 1639
SgrDI CGTCGACG 2 cut(s) 124, 133
SinI GGWCC 5 cut(s) 7, 349, 377, 1531, 1794
SmiMI CAYNNNNRTG 3 cut(s) 1598, 1851, 1964
SmlI CTYRAG 2 cut(s) 1340, 1999
SmoI CTYRAG 2 cut(s) 1340, 1999
SphI GCATGC 1 cut(s) 1011
SseBI AGGCCT 1 cut(s) 737
SsiI CCGC 9 cut(s) 35, 144, 399, 425, 700, 818, 1378, 1826, 2222
SspMI CTAG 5 cut(s) 453, 683, 1115, 1203, 1916
SstI GAGCTC 1 cut(s) 110
StuI AGGCCT 1 cut(s) 737
StyD4I CCNGG 5 cut(s) 345, 357, 1248, 1534, 2175
StyI CCWWGG 3 cut(s) 1053, 1128, 1179
TaiI ACGT 1 cut(s) 1838
TaqI TCGA 8 cut(s) 99, 112, 125, 134, 567, 1473, 1639, 2192
TaqII GACCGA 1 cut(s) 1107
TatI WGTACW 1 cut(s) 2145
TauI GCSGC 3 cut(s) 703, 1381, 1828
TfiI GAWTC 6 cut(s) 890, 1197, 1286, 1618, 1814, 2158
Tru1I TTAA 5 cut(s) 507, 1080, 1236, 1415, 1503
Tru9I TTAA 5 cut(s) 507, 1080, 1236, 1415, 1503
TscAI CASTG 8 cut(s) 49, 1600, 1629, 1775, 1886, 1966, 2031, 2070
TseFI GTSAC 3 cut(s) 1703, 1759, 1808
TseI GCWGC 9 cut(s) 297, 694, 697, 799, 860, 1216, 1459, 1679, 2086
Tsp45I GTSAC 3 cut(s) 1703, 1759, 1808
TspDTI ATGAA 5 cut(s) 630, 732, 1442, 1452, 1796
TspGWI ACGGA 3 cut(s) 1334, 1789, 2001
TspRI CASTG 8 cut(s) 49, 1600, 1629, 1775, 1886, 1966, 2031, 2070
Tth111I GACNNNGTC 1 cut(s) 130
VpaK11BI GGWCC 5 cut(s) 7, 349, 377, 1531, 1794
XapI RAATTY 4 cut(s) 475, 923, 1225, 1635
XceI RCATGY 3 cut(s) 1011, 2082, 2170
XcmI CCANNNNNNNNNTGG 1 cut(s) 1370
XmiI GTMKAC 2 cut(s) 125, 134
XmnI GAANNNNTTC 1 cut(s) 2157
XspI CTAG 5 cut(s) 453, 683, 1115, 1203, 1916
Zsp2I ATGCAT 2 cut(s) 1123, 1656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.