RLG00000020166

guanosine tetraphosphate biosynthetic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011437
Physical Location & Seq
Reverse (-)
61905983 .. 61909077
3095 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020166

Sequence Viewer

Length: 2199 bp
ATGACGGTCCCGATTGCTCTGTACGCAACACCGCCGAGCAGTGTGTGCTCGCACGCCACGTTTGATTTCGAATTGAGCTCCCGATCTTCGTCGTCCACGGCTTCGACGCCGTCCACGTCACAGAAGCCGGCGATGGGGGGCTTGTCGTGCTTGTTCTCGTCTCCGACGGTGAAGCATGCTTCGTCTTCTTCCAGCTTTTCGGGTGGAGGAGGTGAGGAATTGGGGTCGTTGTGGCCTGATAGGGGTGAAGAGTTGAAGGAACTGAGTAGCTCGTTCCGGTATTCGCAGAGCAAGTTCAGTGGGGCTTCAATGAATAGAGATCAGAGCCCGATTTCGGTGTTTCAGGGTCCGGTTTCGAGTAGCAGCAGTGGGGTTTCGAGCTCTGCGAGGAGTCCTCCGATGAGAATTGCGCGGGAAAGGAGTAGCAATGGAGATGTGAACTTGCGGTGCGGTAGTAATGGGTTGTTTAATGGCTTTGTGAGGGGTGCTTTGGGGTCTTCCTGTGTGGATTATGACTCGCCGAGCTTCGAGGTGCGAAATGATGCTTTGGAGATGAGATCATCAGCTGTGCTTGATGAATTGACTTTCAATATGGAGGATGGGTTTGTGGAGGGAAATTTCGAGCCTTATGCAAGTGCGTTGCTTGTAGGTGCGCAGTTGCAGCACAAGATTTTCTATGAAGAGTTTGTTATCAAGGCTTTCTATGAGGCTGAGAAAGCACACAGAGGGCAGATGCGAGCAAGTGGTGATCCGTATTTGCAGCATTGTGTAGAAACGGCGGTGCTGCTCGCATTGATTGGGGCTAATTCCACAGTTGTTGCTGCAGGGCTTTTGCATGACACGCTTGATGATTCTGTTATGAGTTATGACTATATATTTGGAAAATTTGGAGCAGGAGTTGCTGACTTAGTTGAAGGGGTGTCAAAGCTGAGTCATTTGAGCAAGCTTGCCCGTGATAATAATACTGCGTGCAAGACAGTTGAGGCAGACCGTTTGCATACCATGTTTCTTGCCATGGCAGATGCTCGAGCTGTTCTCATTAAATTGGCTGATCGATTGCATAATATGATGACACTGGATGCATTGCCCTTAACTAAACAGCAAAGGTTTGCAAAAGAGACTTTGGAGATCTTTGTGCCATTGGCCCACCGACTAGGAATCTCAAGCTGGAAGGTGCAGCTAGAAAATTTATGTTTTAAGCATCTCAATCCAGACCAACACAATGACCTATCGTCTAAGCTTGAAGATTCATTTGATGATGCAATGATTACTTCTGCCACAGTGAGATTAGACCAAGCTCTTAGAGAAAAAGCGATTTCCTACCATGTACTCTGTGGACGGCATAAGAGCTTGTATAGTATCTACTGCAAAATGTTAAAAAAAAAACTGAACATGAGTGAAATTCATGATATCCATGGGCTACGTTTGATTGTTGAGAAAGAGGAAGATTGCTATCAAGCACTGGAAGTTGTTCGTCAGCTATGGACTGAGGTTCCAGGGAAGTTCAAGGATTACATAACACAACCCAAGTGTAATGGGTATCAGTCACTTCACACTGTGGTCGTGGGTGAAGGCACGGTACCCTTAGAAGTGCAAATTCGAACAAAGGAGATGCATTTGCAAGCTGAATTTGGATTTGCTGCTCATTGGAGATATAAGGAAGGTGATTGTAAGCACCCATCGTTTGTGCTTCAGATGGTTGAGTGGGCAAGATGGGTGGTCACTTGGCAGTGCGAGGCAATGAGCAGAGACCGGTCGTCGATTACCTATGCTGACTCAATCAAGCCACCTTGCACATTTCCTTCTCATTCCGATGACTGCCCTTATTCTTACAAATCCCACTGTGGTGAAGATGAGCCAGTGTTCGTCATCTTGATAGAGAATGACAAGATGTCTGTGCAAGAATTTCCTGCAAACTCGACGATTATGGATCTGGTTGAAAAAGTTGGGCGTGGGAGCATGAGATGGACAACATATGGACTCCCATTGAAGGAAGAACTAAGGCCTAGGCTGAACAAAATGCCAGTGAGTGATCCTACATGCAAACTGCAGATGGGGGATGTAGTGGAGCTGACTCCAGCTATTCCTGACAAGTCATTAACAGAGTACAGGGAAGAGATTCAACGCATGTACGACCGGGGCAGGACTGTATCAAGTGTAGGGTCTCCTGCTAAAAGCATGGTAGGCTGGAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008728 GO:0008893 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009150 GO:0009152 GO:0009163 GO:0009165 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0009991 GO:0010033 GO:0010150 GO:0015969 GO:0015970 GO:0015979 GO:0016020 GO:0016740 GO:0016772 GO:0016778 GO:0016787 GO:0016788 GO:0016794 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0030312 GO:0031667 GO:0032501 GO:0032502 GO:0033865 GO:0033866 GO:0033875 GO:0033993 GO:0034030 GO:0034032 GO:0034033 GO:0034035 GO:0034036 GO:0034404 GO:0034641 GO:0034654 GO:0042221 GO:0042278 GO:0042451 GO:0042455 GO:0042578 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046128 GO:0046129 GO:0046390 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0090693 GO:0097305 GO:0099402 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00230 map00230
Pfam Domains
Protein Families
HD_4 RelA_SpoT

Protein Analysis

733

Amino Acids

81.38

Weight (kDa)

6.09

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD_4 PF13328 233 - 387 2.8e-39 HD domain
HD PF01966 252 - 356 1.4e-08 HD domain
RelA_SpoT PF04607 446 - 555 7.2e-42 Region found in RelA / SpoT proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14050
fragaria_vesca FvH4_6g34880 FvH4_6g34880 FvH4_6g34880
malus_domestica MD09G1181600.v1.1 MD17G1168000.v1.1
prunus_persica Prupe.3G039700_v2.0.a1
pyrus_communis pycom09g09930 pycom17g15960
rosa_chinensis RchiOBHm_Chr2g0145751
rosa_laevigata RLG00000010129 RLG00000020166
rosa_multiflora Rmu_sc0000473.1_g000007
rosa_roxburghii Rroxscaffold_152G00434730 Rroxscaffold_2G00100470
rosa_rugosa Rorug02G0393100
rosa_samantha Rh2AG447400 Rh2BG459700 Rh2CG434200
rosa_wichuraiana Rw2G036560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 654
Acc65I GGTACC 1 cut(s) 1579
AccB1I GGYRCC 1 cut(s) 1579
AccII CGCG 1 cut(s) 412
AciI CCGC 5 cut(s) 32, 412, 445, 450, 779
AclWI GGATC 3 cut(s) 743, 1938, 2027
AcsI RAATTY 7 cut(s) 616, 884, 1186, 1401, 1596, 1628, 1904
AcuI CTGAAG 1 cut(s) 1676
AcyI GRCGYC 1 cut(s) 107
AdeI CACNNNGTG 1 cut(s) 1558
AfaI GTAC 5 cut(s) 23, 1329, 1581, 2108, 2132
AfiI CCNNNNNNNGG 4 cut(s) 134, 242, 334, 1990
AgeI ACCGGT 1 cut(s) 1752
AgsI TTSAA 9 cut(s) 256, 309, 589, 914, 1244, 1507, 1940, 1990, 2123
AhdI GACNNNNNGTC 3 cut(s) 1231, 1756, 1891
AjiI CACGTC 1 cut(s) 117
AjnI CCWGG 1 cut(s) 1495
AleI CACNNNNGTG 1 cut(s) 1845
Alw21I GWGCWC 3 cut(s) 50, 80, 383
Alw26I GTCTC 4 cut(s) 165, 1112, 1743, 2169
AlwI GGATC 3 cut(s) 743, 1938, 2027
Ama87I CYCGRG 1 cut(s) 1026
AoxI GGCC 3 cut(s) 233, 1143, 2003
ApeKI GCWGC 7 cut(s) 363, 661, 760, 784, 821, 1177, 1640
ApoI RAATTY 7 cut(s) 616, 884, 1186, 1401, 1596, 1628, 1904
ArsI GACNNNNNNTTYG 2 cut(s) 860, 892
AsiGI ACCGGT 1 cut(s) 1752
Asp700I GAANNNNTTC 2 cut(s) 1470, 2118
Asp718I GGTACC 1 cut(s) 1579
AspA2I CCTAGG 1 cut(s) 2006
AspLEI GCGC 2 cut(s) 412, 655
AspS9I GGNCC 3 cut(s) 7, 347, 1144
AsuC2I CCSGG 1 cut(s) 2138
AsuHPI GGTGA 7 cut(s) 181, 224, 257, 758, 1580, 1676, 1859
AsuII TTCGAA 2 cut(s) 69, 1600
AvaI CYCGRG 1 cut(s) 1026
AvaII GGWCC 2 cut(s) 7, 347
AvrII CCTAGG 1 cut(s) 2006
BanI GGYRCC 1 cut(s) 1579
BanII GRGCYC 3 cut(s) 80, 329, 383
BarI GAAGNNNNNNTAC 2 cut(s) 1563, 1595
BbsI GAAGAC 2 cut(s) 177, 489
Bbv12I GWGCWC 3 cut(s) 50, 80, 383
BbvI GCAGC 7 cut(s) 375, 673, 771, 772, 808, 1189, 1627
BccI CCATC 7 cut(s) 127, 593, 1687, 1690, 1707, 1959, 2047
BceAI ACGGC 4 cut(s) 94, 114, 792, 1355
BcgI CGANNNNNNTGC 2 cut(s) 611, 645
BciT130I CCWGG 1 cut(s) 1497
BcnI CCSGG 1 cut(s) 2138
BcoDI GTCTC 4 cut(s) 165, 1112, 1743, 2169
BfaI CTAG 3 cut(s) 1154, 1181, 2007
BfmI CTRYAG 2 cut(s) 822, 2048
BglII AGATCT 1 cut(s) 1128
BisI GCNGC 7 cut(s) 364, 662, 761, 785, 822, 1178, 1641
BlnI CCTAGG 1 cut(s) 2006
BlsI GCNGC 7 cut(s) 365, 663, 762, 786, 823, 1179, 1642
Bme1390I CCNGG 2 cut(s) 1497, 2138
Bme18I GGWCC 2 cut(s) 7, 347
BmeRI GACNNNNNGTC 3 cut(s) 1231, 1756, 1891
BmeT110I CYCGRG 1 cut(s) 1026
BmgBI CACGTC 1 cut(s) 117
BmgT120I GGNCC 3 cut(s) 7, 347, 1144
BmiI GGNNCC 4 cut(s) 9, 348, 1494, 1581
BmrFI CCNGG 2 cut(s) 1497, 2138
BmsI GCATC 7 cut(s) 532, 723, 1012, 1069, 1210, 1249, 1602
BpiI GAAGAC 2 cut(s) 177, 489
BplI GAGNNNNNCTC 4 cut(s) 379, 411, 1020, 1052
BpmI CTGGAG 1 cut(s) 2061
Bpu14I TTCGAA 2 cut(s) 69, 1600
BpuEI CTTGAG 1 cut(s) 1147
BpuMI CCSGG 1 cut(s) 2138
Bsa29I ATCGAT 1 cut(s) 1054
BsaBI GATNNNNATC 1 cut(s) 1452
BsaHI GRCGYC 1 cut(s) 107
BsaI GGTCTC 2 cut(s) 1743, 2169
BsaJI CCNNGG 6 cut(s) 96, 1014, 1414, 1496, 2006, 2137
BsaWI WCCGGW 3 cut(s) 276, 349, 1752
BsaXI ACNNNNNCTCC 4 cut(s) 587, 617, 882, 912
Bsc4I CCNNNNNNNGG 4 cut(s) 134, 242, 334, 1990
Bse118I RCCGGY 2 cut(s) 127, 1752
Bse1I ACTGG 4 cut(s) 1080, 1467, 1859, 2024
Bse3DI GCAATG 4 cut(s) 433, 1082, 1269, 1746
Bse8I GATNNNNATC 1 cut(s) 1452
BseBI CCWGG 1 cut(s) 1497
BseCI ATCGAT 1 cut(s) 1054
BseDI CCNNGG 6 cut(s) 96, 1014, 1414, 1496, 2006, 2137
BseGI GGATG 3 cut(s) 604, 1084, 2065
BseJI GATNNNNATC 1 cut(s) 1452
BseLI CCNNNNNNNGG 4 cut(s) 134, 242, 334, 1990
BseMI GCAATG 4 cut(s) 433, 1082, 1269, 1746
BseMII CTCAG 4 cut(s) 254, 702, 920, 1479
BseNI ACTGG 4 cut(s) 1080, 1467, 1859, 2024
BseRI GAGGAG 2 cut(s) 222, 403
BseXI GCAGC 7 cut(s) 375, 673, 771, 772, 808, 1189, 1627
BsgI GTGCAG 1 cut(s) 1196
Bsh1236I CGCG 1 cut(s) 412
Bsh1285I CGRYCG 2 cut(s) 1757, 2137
BshFI GGCC 3 cut(s) 235, 1145, 2005
BshNI GGYRCC 1 cut(s) 1579
BshTI ACCGGT 1 cut(s) 1752
BshVI ATCGAT 1 cut(s) 1054
BsiEI CGRYCG 2 cut(s) 1757, 2137
BsiHKAI GWGCWC 3 cut(s) 50, 80, 383
BsiHKCI CYCGRG 1 cut(s) 1026
BsiSI CCGG 5 cut(s) 128, 277, 350, 1753, 2137
BslI CCNNNNNNNGG 4 cut(s) 134, 242, 334, 1990
BsmAI GTCTC 4 cut(s) 165, 1112, 1743, 2169
BsmBI CGTCTC 1 cut(s) 165
BsnI GGCC 3 cut(s) 235, 1145, 2005
Bso31I GGTCTC 2 cut(s) 1743, 2169
BsoBI CYCGRG 1 cut(s) 1026
Bsp119I TTCGAA 2 cut(s) 69, 1600
Bsp1286I GDGCHC 4 cut(s) 50, 80, 329, 383
Bsp143I GATC 8 cut(s) 83, 319, 557, 748, 1051, 1128, 1930, 2032
Bsp19I CCATGG 2 cut(s) 1014, 1414
BspACI CCGC 5 cut(s) 32, 412, 445, 450, 779
BspANI GGCC 3 cut(s) 235, 1145, 2005
BspCNI CTCAG 4 cut(s) 255, 703, 921, 1480
BspDI ATCGAT 1 cut(s) 1054
BspFNI CGCG 1 cut(s) 412
BspHI TCATGA 1 cut(s) 1405
BspLI GGNNCC 4 cut(s) 9, 348, 1494, 1581
BspMAI CTGCAG 2 cut(s) 826, 2052
BspPI GGATC 3 cut(s) 743, 1938, 2027
BspT104I TTCGAA 2 cut(s) 69, 1600
BspT107I GGYRCC 1 cut(s) 1579
BspTNI GGTCTC 2 cut(s) 1743, 2169
BsrDI GCAATG 4 cut(s) 433, 1082, 1269, 1746
BsrFI RCCGGY 2 cut(s) 127, 1752
BsrI ACTGG 4 cut(s) 1080, 1467, 1859, 2024
BssAI RCCGGY 2 cut(s) 127, 1752
BssECI CCNNGG 6 cut(s) 96, 1014, 1414, 1496, 2006, 2137
BssMI GATC 8 cut(s) 83, 319, 557, 748, 1051, 1128, 1930, 2032
BssNI GRCGYC 1 cut(s) 107
BssT1I CCWWGG 3 cut(s) 1014, 1414, 2006
Bst2UI CCWGG 1 cut(s) 1497
Bst6I CTCTTC 3 cut(s) 243, 675, 2109
BstACI GRCGYC 1 cut(s) 107
BstAPI GCANNNNNTGC 2 cut(s) 45, 899
BstBI TTCGAA 2 cut(s) 69, 1600
BstDEI CTNAG 9 cut(s) 263, 711, 907, 929, 1236, 1301, 1488, 1585, 2000
BstDSI CCRYGG 3 cut(s) 96, 1014, 1414
BstF5I GGATG 3 cut(s) 604, 1084, 2065
BstFNI CGCG 1 cut(s) 412
BstHHI GCGC 2 cut(s) 412, 655
BstKTI GATC 8 cut(s) 86, 322, 560, 751, 1054, 1131, 1933, 2035
BstMAI GTCTC 4 cut(s) 165, 1112, 1743, 2169
BstMBI GATC 8 cut(s) 83, 319, 557, 748, 1051, 1128, 1930, 2032
BstMCI CGRYCG 2 cut(s) 1757, 2137
BstMWI GCNNNNNNNGC 8 cut(s) 23, 45, 147, 661, 716, 841, 899, 2184
BstNI CCWGG 1 cut(s) 1497
BstNSI RCATGY 3 cut(s) 179, 2043, 2131
BstSCI CCNGG 2 cut(s) 1495, 2136
BstSFI CTRYAG 2 cut(s) 822, 2048
BstUI CGCG 1 cut(s) 412
BstV1I GCAGC 7 cut(s) 375, 673, 771, 772, 808, 1189, 1627
BstV2I GAAGAC 2 cut(s) 177, 489
BstX2I RGATCY 2 cut(s) 1128, 1930
BstYI RGATCY 2 cut(s) 1128, 1930
Bsu15I ATCGAT 1 cut(s) 1054
BsuRI GGCC 3 cut(s) 235, 1145, 2005
BsuTUI ATCGAT 1 cut(s) 1054
BtgI CCRYGG 3 cut(s) 96, 1014, 1414
BtgZI GCGATG 1 cut(s) 146
BtrI CACGTC 1 cut(s) 117
BtsCI GGATG 3 cut(s) 604, 1084, 2065
BtsI GCAGTG 3 cut(s) 46, 373, 1736
CciI TCATGA 1 cut(s) 1405
CfoI GCGC 2 cut(s) 412, 655
Cfr10I RCCGGY 2 cut(s) 127, 1752
Cfr13I GGNCC 3 cut(s) 7, 347, 1144
ClaI ATCGAT 1 cut(s) 1054
CseI GACGC 1 cut(s) 115
Csp6I GTAC 5 cut(s) 22, 1328, 1580, 2107, 2131
CspAI ACCGGT 1 cut(s) 1752
CspCI CAANNNNNGTGG 6 cut(s) 280, 315, 799, 834, 1698, 1733
CviQI GTAC 5 cut(s) 22, 1328, 1580, 2107, 2131
DdeI CTNAG 9 cut(s) 263, 711, 907, 929, 1236, 1301, 1488, 1585, 2000
DpnI GATC 8 cut(s) 85, 321, 559, 750, 1053, 1130, 1932, 2034
DpnII GATC 8 cut(s) 83, 319, 557, 748, 1051, 1128, 1930, 2032
DraIII CACNNNGTG 1 cut(s) 1558
DriI GACNNNNNGTC 3 cut(s) 1231, 1756, 1891
Eam1104I CTCTTC 3 cut(s) 243, 675, 2109
Eam1105I GACNNNNNGTC 3 cut(s) 1231, 1756, 1891
EarI CTCTTC 3 cut(s) 243, 675, 2109
Ecl136II GAGCTC 2 cut(s) 78, 381
Eco130I CCWWGG 3 cut(s) 1014, 1414, 2006
Eco147I AGGCCT 1 cut(s) 2005
Eco24I GRGCYC 3 cut(s) 80, 329, 383
Eco31I GGTCTC 2 cut(s) 1743, 2169
Eco32I GATATC 1 cut(s) 1411
Eco47I GGWCC 2 cut(s) 7, 347
Eco53kI GAGCTC 2 cut(s) 78, 381
Eco57I CTGAAG 1 cut(s) 1676
Eco88I CYCGRG 1 cut(s) 1026
EcoICRI GAGCTC 2 cut(s) 78, 381
EcoRII CCWGG 1 cut(s) 1495
EcoRV GATATC 1 cut(s) 1411
EcoT14I CCWWGG 3 cut(s) 1014, 1414, 2006
EcoT22I ATGCAT 2 cut(s) 1084, 1617
EcoT38I GRGCYC 3 cut(s) 80, 329, 383
ErhI CCWWGG 3 cut(s) 1014, 1414, 2006
Esp3I CGTCTC 1 cut(s) 165
FauI CCCGC 1 cut(s) 405
FauNDI CATATG 1 cut(s) 1975
Fnu4HI GCNGC 7 cut(s) 364, 662, 761, 785, 822, 1178, 1641
FokI GGATG 3 cut(s) 611, 1091, 2072
FriOI GRGCYC 3 cut(s) 80, 329, 383
Fsp4HI GCNGC 7 cut(s) 364, 662, 761, 785, 822, 1178, 1641
FspBI CTAG 3 cut(s) 1154, 1181, 2007
FspI TGCGCA 1 cut(s) 654
GlaI GCGC 2 cut(s) 411, 654
GluI GCNGC 7 cut(s) 364, 662, 761, 785, 822, 1178, 1641
GsuI CTGGAG 1 cut(s) 2061
HaeIII GGCC 3 cut(s) 235, 1145, 2005
HapII CCGG 5 cut(s) 128, 277, 350, 1753, 2137
HgaI GACGC 1 cut(s) 115
HhaI GCGC 2 cut(s) 412, 655
Hin1I GRCGYC 1 cut(s) 107
Hin6I GCGC 2 cut(s) 410, 653
HinP1I GCGC 2 cut(s) 410, 653
HindIII AAGCTT 2 cut(s) 944, 1238
HpaII CCGG 5 cut(s) 128, 277, 350, 1753, 2137
HphI GGTGA 7 cut(s) 181, 224, 257, 758, 1580, 1676, 1859
Hpy166II GTNNAC 4 cut(s) 96, 114, 439, 1337
Hpy188I TCNGA 5 cut(s) 165, 324, 399, 1695, 1813
Hpy188III TCNNGA 6 cut(s) 10, 81, 1211, 1406, 1873, 2087
Hpy8I GTNNAC 4 cut(s) 96, 114, 439, 1337
Hpy99I CGWCG 5 cut(s) 94, 109, 169, 1762, 1924
HpyAV CCTTC 7 cut(s) 250, 908, 1165, 1565, 1655, 1812, 1984
HpyCH4IV ACGT 3 cut(s) 59, 116, 1423
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 45, 147, 661, 716, 841, 899, 2184
HpyF3I CTNAG 9 cut(s) 263, 711, 907, 929, 1236, 1301, 1488, 1585, 2000
HpySE526I ACGT 3 cut(s) 59, 116, 1423
Hsp92I GRCGYC 1 cut(s) 107
HspAI GCGC 2 cut(s) 410, 653
KpnI GGTACC 1 cut(s) 1583
KroI GCCGGC 1 cut(s) 127
KroNI GCCGGC 1 cut(s) 129
Kzo9I GATC 8 cut(s) 83, 319, 557, 748, 1051, 1128, 1930, 2032
LmnI GCTCC 4 cut(s) 83, 890, 1956, 2068
Lsp1109I GCAGC 7 cut(s) 375, 673, 771, 772, 808, 1189, 1627
LweI GCATC 7 cut(s) 532, 723, 1012, 1069, 1210, 1249, 1602
MaeI CTAG 3 cut(s) 1154, 1181, 2007
MaeII ACGT 3 cut(s) 59, 116, 1423
MaeIII GTNAC 3 cut(s) 117, 1545, 1720
MalI GATC 8 cut(s) 85, 321, 559, 750, 1053, 1130, 1932, 2034
MboI GATC 8 cut(s) 83, 319, 557, 748, 1051, 1128, 1930, 2032
MflI RGATCY 2 cut(s) 1128, 1930
MhlI GDGCHC 4 cut(s) 50, 80, 329, 383
MlyI GAGTC 6 cut(s) 400, 509, 940, 1769, 1974, 2068
MmeI TCCRAC 1 cut(s) 188
Mph1103I ATGCAT 2 cut(s) 1084, 1617
MroNI GCCGGC 1 cut(s) 127
MroXI GAANNNNTTC 2 cut(s) 1470, 2118
MseI TTAA 6 cut(s) 468, 1041, 1091, 1197, 1376, 2099
MslI CAYNNNNRTG 2 cut(s) 1812, 1845
MspA1I CMGCKG 1 cut(s) 566
MspI CCGG 5 cut(s) 128, 277, 350, 1753, 2137
MspR9I CCNGG 2 cut(s) 1497, 2138
MvaI CCWGG 1 cut(s) 1497
MvnI CGCG 1 cut(s) 412
MwoI GCNNNNNNNGC 8 cut(s) 23, 45, 147, 661, 716, 841, 899, 2184
NaeI GCCGGC 1 cut(s) 129
NciI CCSGG 1 cut(s) 2138
NcoI CCATGG 2 cut(s) 1014, 1414
NdeI CATATG 1 cut(s) 1975
NdeII GATC 8 cut(s) 83, 319, 557, 748, 1051, 1128, 1930, 2032
NgoMIV GCCGGC 1 cut(s) 127
NlaIV GGNNCC 4 cut(s) 9, 348, 1494, 1581
NmeAIII GCCGAG 2 cut(s) 60, 546
NmuCI GTSAC 3 cut(s) 117, 1545, 1720
NsbI TGCGCA 1 cut(s) 654
NsiI ATGCAT 2 cut(s) 1084, 1617
NspI RCATGY 3 cut(s) 179, 2043, 2131
NspV TTCGAA 2 cut(s) 69, 1600
OliI CACNNNNGTG 1 cut(s) 1845
PaeI GCATGC 1 cut(s) 179
PaeR7I CTCGAG 1 cut(s) 1026
PagI TCATGA 1 cut(s) 1405
PceI AGGCCT 1 cut(s) 2005
PcsI WCGNNNNNNNCGW 3 cut(s) 56, 113, 383
PdiI GCCGGC 1 cut(s) 129
PdmI GAANNNNTTC 2 cut(s) 1470, 2118
PfeI GAWTC 4 cut(s) 851, 1158, 1247, 2119
PflFI GACNNNGTC 1 cut(s) 109
PinAI ACCGGT 1 cut(s) 1752
PkrI GCNGC 7 cut(s) 365, 663, 762, 786, 823, 1179, 1642
PleI GAGTC 6 cut(s) 399, 509, 939, 1769, 1974, 2068
PpsI GAGTC 6 cut(s) 399, 509, 939, 1769, 1974, 2068
Psp124BI GAGCTC 2 cut(s) 80, 383
Psp6I CCWGG 1 cut(s) 1495
PspGI CCWGG 1 cut(s) 1495
PspN4I GGNNCC 4 cut(s) 9, 348, 1494, 1581
PspPI GGNCC 3 cut(s) 7, 347, 1144
PspXI VCTCGAGB 1 cut(s) 1026
PstI CTGCAG 2 cut(s) 826, 2052
PsuI RGATCY 2 cut(s) 1128, 1930
PsyI GACNNNGTC 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 566
RsaI GTAC 5 cut(s) 23, 1329, 1581, 2108, 2132
RsaNI GTAC 5 cut(s) 22, 1328, 1580, 2107, 2131
RseI CAYNNNNRTG 2 cut(s) 1812, 1845
SacI GAGCTC 2 cut(s) 80, 383
SaqAI TTAA 6 cut(s) 468, 1041, 1091, 1197, 1376, 2099
SatI GCNGC 7 cut(s) 364, 662, 761, 785, 822, 1178, 1641
Sau3AI GATC 8 cut(s) 83, 319, 557, 748, 1051, 1128, 1930, 2032
Sau96I GGNCC 3 cut(s) 7, 347, 1144
SchI GAGTC 6 cut(s) 400, 509, 940, 1769, 1974, 2068
ScrFI CCNGG 2 cut(s) 1497, 2138
SduI GDGCHC 4 cut(s) 50, 80, 329, 383
SfaNI GCATC 7 cut(s) 532, 723, 1012, 1069, 1210, 1249, 1602
SfcI CTRYAG 2 cut(s) 822, 2048
Sfr274I CTCGAG 1 cut(s) 1026
SfuI TTCGAA 2 cut(s) 69, 1600
SinI GGWCC 2 cut(s) 7, 347
SlaI CTCGAG 1 cut(s) 1026
SmiMI CAYNNNNRTG 2 cut(s) 1812, 1845
SmlI CTYRAG 2 cut(s) 1026, 1162
SmoI CTYRAG 2 cut(s) 1026, 1162
SphI GCATGC 1 cut(s) 179
SseBI AGGCCT 1 cut(s) 2005
SsiI CCGC 5 cut(s) 32, 412, 445, 450, 779
SspMI CTAG 3 cut(s) 1154, 1181, 2007
SstI GAGCTC 2 cut(s) 80, 383
StuI AGGCCT 1 cut(s) 2005
StyD4I CCNGG 2 cut(s) 1495, 2136
StyI CCWWGG 3 cut(s) 1014, 1414, 2006
TaiI ACGT 3 cut(s) 62, 119, 1426
TatI WGTACW 2 cut(s) 1327, 2106
TfiI GAWTC 4 cut(s) 851, 1158, 1247, 2119
Tru1I TTAA 6 cut(s) 468, 1041, 1091, 1197, 1376, 2099
Tru9I TTAA 6 cut(s) 468, 1041, 1091, 1197, 1376, 2099
TseFI GTSAC 3 cut(s) 117, 1545, 1720
TseI GCWGC 7 cut(s) 363, 661, 760, 784, 821, 1177, 1640
Tsp45I GTSAC 3 cut(s) 117, 1545, 1720
TspDTI ATGAA 5 cut(s) 326, 591, 693, 1239, 1394
TspGWI ACGGA 1 cut(s) 741
Tth111I GACNNNGTC 1 cut(s) 109
VpaK11BI GGWCC 2 cut(s) 7, 347
XapI RAATTY 7 cut(s) 616, 884, 1186, 1401, 1596, 1628, 1904
XceI RCATGY 3 cut(s) 179, 2043, 2131
XcmI CCANNNNNNNNNTGG 1 cut(s) 1331
XhoI CTCGAG 1 cut(s) 1026
XmaJI CCTAGG 1 cut(s) 2006
XmnI GAANNNNTTC 2 cut(s) 1470, 2118
XspI CTAG 3 cut(s) 1154, 1181, 2007
Zsp2I ATGCAT 2 cut(s) 1084, 1617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.