Rorug02G0393100

guanosine tetraphosphate biosynthetic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0011437
Physical Location & Seq
Reverse (-)
50362968 .. 50372389
9422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0393100.1

Sequence Viewer

Length: 2901 bp
ATGTGTTTACAGCCTCTCCGACTGGAAATCAAGAGGAAACTAGCTCAAAGATCAGAGAGAGTCAAATCTGTGGATCTACATCCAACAGAACCATGGATATTGGCGAGTCTTTATTCAGGAACAGTGTGTATTTGGAACTACCAGACTCAGACAATGGCCAAGTCATTTGAGGTCACTGAGTTGCCAGTTAGGTCAGCCAAATTTATATCACGCAAGCAATGGGTTGTTGCTGGAGCTGACGATATGTTTATTCGTGTATACAACTACAACACAATGGATAAGGTTAAAGTATTTGAGGCCCATACTGACTACATTAGATGTGTGGCTGTCCATCCTACGCTCCCATATGTGTTATCATCATCTGATGATATGCTCATCAAGCTATGGGATTGGGACAAGGGTTGGATTTGTACTCAAATATTTGAAGGACATTCGCATTATGTGATGCAAGTGACATTTAATCCTAAAGATACCAACACCTTTGCAAGTGCGTCTCTTGATCGCACCATAAAGATATGGAATCTAGGCTCCCCTGACCCAAATTTTACACTGGATGCCCACCAGAAAGGTGTAAATTGCGTTGATTACTTTACGGGTGGTGATAAACCTTATCTCATTACCGGTTCCGATGATCACACTGCTAAGGTGTGGGACTATCAAACAAAAGGTTGTGTCCAGACACTTGAAGGCCATACACACAATGTTTCTGCTGTATGTTTTCATCCTGAACTCCCTATAATAATAACAGGGTCAGAGGACGGAACAGTACGAATATGGCATTCAACCACTTACAGGCTTGAGAACACATTGAATTATGGGCTTGAAAGAGTTTGGGCCATTGGATACATGAAGGGCTCACGACGGGTTGTGATTGGTTATGATGAAGGAACCATTATGGTAAAACTTGGACGTGAAGTACCTGTTGCTAGTATGGACAATGGTGGAAAGATCATTTGGGCTAAGCACAATGAAATTCAAACAGTGAACATTAAGAGTGTAGGCGCTGATTATGAGGTTACTGATGGAGAGAGATTGCCATTGGCCGTCAAGGAGTTGGGCACATGTGATCTTTACCCCCAAAGCTTAAAGCACAACCCCAATGGGAGGTTTGTTGTAGTTTGTGGAGATGGAGAATACATTATATATACTGCTTTGGCCTGGAGAAATCGGTCGTTTGGTTCTGCTTTGGAATTTGCTTGGTCATCTGAAGGAGAGTATGCTGTTAGAGAGAGTACTTCAAAAATTAAAATTTTCACTAAGAACTTCCAGGAGAAAAGGAGTATTCGACCAACATTTTCAGCAGAACATATATATGGGGGAACTTTATTGGCGATGTGCTCAAATGATTTTATTTGCTTTTATGATTGGGCTGAGTGCAGATTGATTCGGAGAATAGATGTGAATGTGAAAAATCTCTATTGGGCTGATAGTGGCGATTTAGTTGCTATTGCCAGTGACGCATCATTCTACATCCTGAAGTACAATCGAGATGTAGTCTCTTCTTATTTGGATAGCGGAAGACCTGTAGATGAACAAGGTGTAGAAGATGCATTTGAGCTTCTCCATGAAATGAATGAACGGGTCAGGACTGGTTTATGGGTTGGGGATTGCTTCATTTACAACAACTCGTCTTGGAGGCTTAATTATTGTGTTGGTGGAGAGGTAACTACAATGTTCCATTTAGACCGGCCAATGTACTTGTTAGGCTATCTTGCCAATCAAAGTCGGGTGTATCTGATAGACAAAGAGTTCAATGTGATGGGGTACACACTACTTTTGAGTTTGATTGAGTATAAGACACTTGTTATGCGTGGAGACCTTGAAAGGGCCAATGAACTTTTACCTTCAATTCCTAAAGACCATCATAACAGTGTGGCTCGTTTCTTGGAATCACGTGGTATGATAGAGGATGCACTTGAAGTTGCTACAGATCCTGATTACAGATTTGAGCTAGCCATTCAGCTTGGTAGATTAGAAATTGCAAAGGAAATTGCCAGTGAGGTGCAGAGTGAATCTAAATGGAAACAGCTAGGTGAACTAGCCATGTCTACCGGAAAGTTGGAAATGGCTGAGCAATGTTTGAAGCATGCCATGGACTTGAGTGGTTTATTACTGCTTTATTCATCTCTTGGAGATGCTGAGGGAATCTCAGGACTTGCATCCCTTGCTAAAGAACAGGGGAAGAACAATGTTGCATTCCTTTGTTTATTCATGTTGGGTCGATTGGAAGAGTGCCTTGAGCTGTTAGTCGAAAGCAATCGGATACCTGAGGCTGCTTTGATGGCTCGATCATATCTTCCAAGCAAGGTCTCAGAAATAGTAGCTATTTGGAGAAAAGACCTTAGCAAGGTTAATCCAAAAGCTGCTGAATCTTTGGCTGATCCTGAAGAGTATCCTAATTTGTTTGAGGATTGGCAAGTTGCACTCTCGGTTGAATCTAGAGCTGCAGAGACAAGGGGTGTTTATCCTCCTGCAGAGGAGTACGTGAAACATGTTGATAAAACACACATGACCCTTGTGGAGGCTTTCAGGAACTTGCAAGTGGATGAAGAGAACTTGCAAATGGATGAAGAGCAGCCCTGTGAAAATGGAGACTCAACGCATGAGGTTTCTGAACTGAATGGGGAACAGACTGTAGAGGAACATGCAGCTGACCAGCACGAGGAAGGGAGCCAGGAGGAGGCTGTCGTGGTGGATGCTGATTCTACGGATGGCACTGTACTCATTAATGGTAACGAAGCTGAAGAAGACTGGGTTCATTGTGTATACCTTACTAGTCTGGATTCTTTTGATATCTCGATGAGTGCTTACACCACGTCACTAGGTTCCTATAAAGACTGCTGGTCCAGCTGTATGGATCCTTTATTAAAGGTCTCTTGCAAGAGGCTGACAAAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008728 GO:0008893 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009150 GO:0009152 GO:0009163 GO:0009165 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0009991 GO:0010033 GO:0010150 GO:0015969 GO:0015970 GO:0015979 GO:0016020 GO:0016740 GO:0016772 GO:0016778 GO:0016787 GO:0016788 GO:0016794 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0030312 GO:0031667 GO:0032501 GO:0032502 GO:0033865 GO:0033866 GO:0033875 GO:0033993 GO:0034030 GO:0034032 GO:0034033 GO:0034035 GO:0034036 GO:0034404 GO:0034641 GO:0034654 GO:0042221 GO:0042278 GO:0042451 GO:0042455 GO:0042578 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046128 GO:0046129 GO:0046390 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0090693 GO:0097305 GO:0099402 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00230 map00230
Pfam Domains
Protein Families
HD_4 RelA_SpoT

Protein Analysis

966

Amino Acids

109.41

Weight (kDa)

5.01

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 6 - 174 3.3e-17 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 10 - 94 4e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 12 - 160 3e-17 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 15 - 175 6.4e-27 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 19 - 177 2.5e-09 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 19 - 188 3.1e-20 Prp19 WD40 domain
WDR55 PF24796 19 - 202 6.5e-15 WDR55
Beta-prop_EML_2 PF23414 23 - 175 5.7e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 34 - 173 5.2e-06 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 35 - 220 3.9e-16 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 38 - 196 3.2e-17 CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_1st PF24780 42 - 176 7.3e-06 MABP1/WDR62 first WD40 domain
WD40_WDHD1_1st PF24817 61 - 134 5.4e-11 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 64 - 195 9.7e-21 THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 71 - 175 6.7e-08 MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd PF25047 72 - 263 6.6e-14 TEP-1 second beta-propeller
Beta-prop_EIPR1 PF23609 82 - 173 3.2e-06 EIPR1 beta-propeller
WD40 PF00400 94 - 130 4e-08 WD domain, G-beta repeat
WD40 PF00400 136 - 174 7.7e-07 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 138 - 299 3.3e-27 WDR3 first beta-propeller domain
WDR55 PF24796 141 - 299 7.7e-16 WDR55
EIF3I PF24805 141 - 232 8.2e-06 EIF3I
Beta-prop_RIG_2nd PF23775 142 - 249 9.1e-06 RIG second beta-propeller
Beta-prop_WDR3_2nd PF25172 143 - 263 3.1e-15 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 157 - 292 1.4e-29 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 159 - 260 3e-06 MABP1/WDR62 second WD40 domain
Beta-prop_EML PF23409 167 - 244 1.7e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 170 - 259 7.2e-06 WDR36/Utp21 first beta-propeller
WD40 PF00400 180 - 218 5.2e-07 WD domain, G-beta repeat
WD40_Prp19 PF24814 186 - 270 1.9e-15 Prp19 WD40 domain
Beta-prop_SCAP PF24017 186 - 262 4e-06 SCAP Beta-propeller
Beta-prop_THOC3 PF25174 187 - 298 8e-16 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 200 - 267 4.1e-06 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 201 - 287 5.6e-08 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 201 - 271 3.8e-11 WDHD1 first WD40 domain
Beta-prop_WDR75_1st PF23869 201 - 268 3.1e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_EML_2 PF23414 202 - 299 1.8e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta PF25391 202 - 295 5.4e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd PF23393 204 - 261 6e-07 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 209 - 264 1.9e-06 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 224 - 259 4.2e-11 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 237 - 301 2.5e-06 Echinoderm microtubule-associated protein second beta-propeller
B-prop_COPA_B_2nd PF04053 322 - 581 1.3e-89 COPA/B second beta-propeller
TPR_COPA_B PF23953 598 - 778 6.5e-82 COPA/B TPR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14050
fragaria_vesca FvH4_6g34880 FvH4_6g34880 FvH4_6g34880
malus_domestica MD09G1181600.v1.1 MD17G1168000.v1.1
prunus_persica Prupe.3G039700_v2.0.a1
pyrus_communis pycom09g09930 pycom17g15960
rosa_chinensis RchiOBHm_Chr2g0145751
rosa_laevigata RLG00000010129 RLG00000020166
rosa_multiflora Rmu_sc0000473.1_g000007
rosa_roxburghii Rroxscaffold_152G00434730 Rroxscaffold_2G00100470
rosa_rugosa Rorug02G0393100
rosa_samantha Rh2AG447400 Rh2BG459700 Rh2CG434200
rosa_wichuraiana Rw2G036560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 258, 2048, 2766
AciI CCGC 1 cut(s) 1515
AclWI GGATC 5 cut(s) 81, 1925, 2375, 2852, 2865
AcoI YGGCCR 3 cut(s) 156, 1041, 1688
AcsI RAATTY 5 cut(s) 200, 541, 972, 1190, 1248
AcuI CTGAAG 4 cut(s) 1227, 1496, 2406, 2763
AcvI CACGTG 1 cut(s) 1895
AfaI GTAC 9 cut(s) 412, 768, 918, 1234, 1481, 1697, 1766, 2483, 2721
AfiI CCNNNNNNNGG 7 cut(s) 792, 1104, 1825, 2347, 2521, 2662, 2680
AflIII ACRYGT 2 cut(s) 1061, 2491
AgeI ACCGGT 1 cut(s) 620
AhdI GACNNNNNGTC 1 cut(s) 2842
AhlI ACTAGT 1 cut(s) 2774
AjiI CACGTC 2 cut(s) 911, 2817
AjnI CCWGG 3 cut(s) 1157, 1266, 2673
AjuI GAANNNNNNNTTGG 2 cut(s) 937, 969
Alw21I GWGCWC 1 cut(s) 1340
Alw26I GTCTC 7 cut(s) 498, 1501, 1809, 2314, 2444, 2586, 2878
AlwI GGATC 5 cut(s) 81, 1925, 2375, 2852, 2865
AlwNI CAGNNNCTG 1 cut(s) 1934
AoxI GGCC 8 cut(s) 156, 297, 688, 834, 1041, 1155, 1688, 1827
ApeKI GCWGC 5 cut(s) 2273, 2363, 2444, 2575, 2648
ApoI RAATTY 5 cut(s) 200, 541, 972, 1190, 1248
AseI ATTAAT 1 cut(s) 2727
AsiGI ACCGGT 1 cut(s) 620
AspLEI GCGC 1 cut(s) 1004
AspS9I GGNCC 4 cut(s) 298, 834, 1827, 2844
AsuHPI GGTGA 2 cut(s) 611, 2045
AsuNHI GCTAGC 1 cut(s) 1951
AvaII GGWCC 1 cut(s) 2844
AxyI CCTNAGG 1 cut(s) 2268
BaeGI GKGCMC 1 cut(s) 1061
BalI TGGCCA 1 cut(s) 158
BamHI GGATCC 1 cut(s) 2857
BanII GRGCYC 1 cut(s) 857
BauI CACGAG 1 cut(s) 2660
BbrPI CACGTG 1 cut(s) 1895
BbsI GAAGAC 2 cut(s) 1525, 2754
Bbv12I GWGCWC 1 cut(s) 1340
BbvCI CCTCAGC 1 cut(s) 2139
BbvI GCAGC 5 cut(s) 2260, 2350, 2431, 2587, 2660
BccI CCATC 7 cut(s) 339, 1016, 1121, 1753, 1869, 2275, 2705
BceAI ACGGC 1 cut(s) 1028
BcgI CGANNNNNNTGC 2 cut(s) 1424, 1458
BciT130I CCWGG 3 cut(s) 1159, 1268, 2675
BciVI GTATCC 3 cut(s) 836, 2256, 2403
BclI TGATCA 1 cut(s) 631
BcoDI GTCTC 7 cut(s) 498, 1501, 1809, 2314, 2444, 2586, 2878
BcuI ACTAGT 1 cut(s) 2774
BfaI CTAG 9 cut(s) 41, 524, 927, 1952, 2030, 2039, 2439, 2775, 2822
BfmI CTRYAG 5 cut(s) 1524, 1926, 2445, 2472, 2634
BfoI RGCGCY 1 cut(s) 1005
BfuI GTATCC 3 cut(s) 836, 2256, 2403
BisI GCNGC 5 cut(s) 2274, 2364, 2445, 2576, 2649
BlpI GCTNAGC 2 cut(s) 960, 2070
BlsI GCNGC 5 cut(s) 2275, 2365, 2446, 2577, 2650
BmcAI AGTACT 1 cut(s) 1234
Bme1390I CCNGG 3 cut(s) 1159, 1268, 2675
Bme18I GGWCC 1 cut(s) 2844
BmeRI GACNNNNNGTC 1 cut(s) 2842
BmgBI CACGTC 2 cut(s) 911, 2817
BmgT120I GGNCC 4 cut(s) 298, 834, 1827, 2844
BmiI GGNNCC 6 cut(s) 529, 625, 889, 2672, 2827, 2859
BmrFI CCNGG 3 cut(s) 1159, 1268, 2675
BmrI ACTGGG 1 cut(s) 2761
BmsI GCATC 8 cut(s) 435, 544, 1469, 1537, 1900, 2125, 2168, 2686
BmtI GCTAGC 1 cut(s) 1955
BmuI ACTGGG 1 cut(s) 2761
BpiI GAAGAC 2 cut(s) 1525, 2754
BplI GAGNNNNNCTC 2 cut(s) 2132, 2164
BpmI CTGGAG 2 cut(s) 252, 1180
Bpu10I CCTNAGC 3 cut(s) 642, 2139, 2342
Bpu1102I GCTNAGC 2 cut(s) 960, 2070
BpuEI CTTGAG 3 cut(s) 818, 2119, 2258
BsaAI YACGTR 2 cut(s) 1895, 2485
BsaBI GATNNNNATC 1 cut(s) 78
BsaI GGTCTC 3 cut(s) 1809, 2314, 2878
BsaJI CCNNGG 2 cut(s) 92, 2091
BsaWI WCCGGW 2 cut(s) 620, 2051
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 7 cut(s) 792, 1104, 1825, 2347, 2521, 2662, 2680
Bse118I RCCGGY 2 cut(s) 620, 1686
Bse1I ACTGG 7 cut(s) 27, 185, 555, 1452, 1594, 1995, 2756
Bse21I CCTNAGG 1 cut(s) 2268
Bse3DI GCAATG 2 cut(s) 224, 2081
Bse8I GATNNNNATC 1 cut(s) 78
BseBI CCWGG 3 cut(s) 1159, 1268, 2675
BseDI CCNNGG 2 cut(s) 92, 2091
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 7 cut(s) 792, 1104, 1825, 2347, 2521, 2662, 2680
BseMI GCAATG 2 cut(s) 224, 2081
BseMII CTCAG 8 cut(s) 161, 168, 1362, 2061, 2130, 2163, 2259, 2325
BseNI ACTGG 7 cut(s) 27, 185, 555, 1452, 1594, 1995, 2756
BseRI GAGGAG 2 cut(s) 2492, 2693
BseSI GKGCMC 1 cut(s) 1061
BseXI GCAGC 5 cut(s) 2260, 2350, 2431, 2587, 2660
BsgI GTGCAG 2 cut(s) 1396, 2024
Bsh1285I CGRYCG 1 cut(s) 1172
BshFI GGCC 8 cut(s) 158, 299, 690, 836, 1043, 1157, 1690, 1829
BshTI ACCGGT 1 cut(s) 620
BsiEI CGRYCG 1 cut(s) 1172
BsiHKAI GWGCWC 1 cut(s) 1340
BsiSI CCGG 3 cut(s) 621, 1687, 2052
BslFI GGGAC 2 cut(s) 407, 665
BslI CCNNNNNNNGG 7 cut(s) 792, 1104, 1825, 2347, 2521, 2662, 2680
BsmAI GTCTC 7 cut(s) 498, 1501, 1809, 2314, 2444, 2586, 2878
BsmBI CGTCTC 1 cut(s) 498
BsmFI GGGAC 2 cut(s) 407, 665
BsmI GAATGC 2 cut(s) 778, 2195
BsnI GGCC 8 cut(s) 158, 299, 690, 836, 1043, 1157, 1690, 1829
Bso31I GGTCTC 3 cut(s) 1809, 2314, 2878
Bsp1286I GDGCHC 3 cut(s) 857, 1061, 1340
Bsp1720I GCTNAGC 2 cut(s) 960, 2070
Bsp19I CCATGG 2 cut(s) 92, 2091
BspACI CCGC 1 cut(s) 1515
BspANI GGCC 8 cut(s) 158, 299, 690, 836, 1043, 1157, 1690, 1829
BspCNI CTCAG 8 cut(s) 160, 169, 1363, 2062, 2131, 2162, 2260, 2324
BspLI GGNNCC 6 cut(s) 529, 625, 889, 2672, 2827, 2859
BspMAI CTGCAG 2 cut(s) 2449, 2476
BspOI GCTAGC 1 cut(s) 1955
BspPI GGATC 5 cut(s) 81, 1925, 2375, 2852, 2865
BspQI GCTCTTC 1 cut(s) 2565
BspTNI GGTCTC 3 cut(s) 1809, 2314, 2878
BsrDI GCAATG 2 cut(s) 224, 2081
BsrFI RCCGGY 2 cut(s) 620, 1686
BsrI ACTGG 7 cut(s) 27, 185, 555, 1452, 1594, 1995, 2756
BssAI RCCGGY 2 cut(s) 620, 1686
BssECI CCNNGG 2 cut(s) 92, 2091
BssNAI GTATAC 2 cut(s) 259, 2767
BssSI CACGAG 1 cut(s) 2660
BssT1I CCWWGG 2 cut(s) 92, 2091
Bst1107I GTATAC 2 cut(s) 259, 2767
Bst2BI CACGAG 1 cut(s) 2660
Bst2UI CCWGG 3 cut(s) 1159, 1268, 2675
Bst4CI ACNGT 6 cut(s) 124, 766, 982, 1871, 2635, 2719
Bst6I CTCTTC 5 cut(s) 1504, 2223, 2382, 2544, 2565
BstAPI GCANNNNNTGC 1 cut(s) 2165
BstBAI YACGTR 2 cut(s) 1895, 2485
BstC8I GCNNGC 3 cut(s) 215, 1953, 2088
BstDSI CCRYGG 2 cut(s) 92, 2091
BstENI CCTNNNNNAGG 2 cut(s) 2345, 2519
BstH2I RGCGCY 1 cut(s) 1005
BstHHI GCGC 1 cut(s) 1004
BstMAI GTCTC 7 cut(s) 498, 1501, 1809, 2314, 2444, 2586, 2878
BstMCI CGRYCG 1 cut(s) 1172
BstMWI GCNNNNNNNGC 5 cut(s) 379, 1457, 2165, 2282, 2847
BstNI CCWGG 3 cut(s) 1159, 1268, 2675
BstNSI RCATGY 4 cut(s) 1065, 2090, 2495, 2648
BstSCI CCNGG 3 cut(s) 1157, 1266, 2673
BstSFI CTRYAG 5 cut(s) 1524, 1926, 2445, 2472, 2634
BstSLI GKGCMC 1 cut(s) 1061
BstV1I GCAGC 5 cut(s) 2260, 2350, 2431, 2587, 2660
BstV2I GAAGAC 2 cut(s) 1525, 2754
BstX2I RGATCY 3 cut(s) 73, 1930, 2857
BstXI CCANNNNNNTGG 1 cut(s) 2854
BstYI RGATCY 3 cut(s) 73, 1930, 2857
BstZ17I GTATAC 2 cut(s) 259, 2767
Bsu36I CCTNAGG 1 cut(s) 2268
BsuI GTATCC 3 cut(s) 836, 2256, 2403
BsuRI GGCC 8 cut(s) 158, 299, 690, 836, 1043, 1157, 1690, 1829
BtgI CCRYGG 2 cut(s) 92, 2091
BtgZI GCGATG 1 cut(s) 1346
BtrI CACGTC 2 cut(s) 911, 2817
BtsI GCAGTG 1 cut(s) 636
BtsIMutI CAGTG 9 cut(s) 129, 174, 548, 636, 987, 1459, 1876, 2002, 2715
Cac8I GCNNGC 3 cut(s) 215, 1953, 2088
CaiI CAGNNNCTG 1 cut(s) 1934
CfoI GCGC 1 cut(s) 1004
Cfr10I RCCGGY 2 cut(s) 620, 1686
Cfr13I GGNCC 4 cut(s) 298, 834, 1827, 2844
CseI GACGC 2 cut(s) 480, 1466
Csp6I GTAC 9 cut(s) 411, 767, 917, 1233, 1480, 1696, 1765, 2482, 2720
CspAI ACCGGT 1 cut(s) 620
CviQI GTAC 9 cut(s) 411, 767, 917, 1233, 1480, 1696, 1765, 2482, 2720
DriI GACNNNNNGTC 1 cut(s) 2842
EaeI YGGCCR 3 cut(s) 156, 1041, 1688
Eam1104I CTCTTC 5 cut(s) 1504, 2223, 2382, 2544, 2565
Eam1105I GACNNNNNGTC 1 cut(s) 2842
EarI CTCTTC 5 cut(s) 1504, 2223, 2382, 2544, 2565
Eco130I CCWWGG 2 cut(s) 92, 2091
Eco24I GRGCYC 1 cut(s) 857
Eco31I GGTCTC 3 cut(s) 1809, 2314, 2878
Eco32I GATATC 1 cut(s) 2794
Eco47I GGWCC 1 cut(s) 2844
Eco57I CTGAAG 4 cut(s) 1227, 1496, 2406, 2763
Eco72I CACGTG 1 cut(s) 1895
Eco81I CCTNAGG 1 cut(s) 2268
EcoNI CCTNNNNNAGG 2 cut(s) 2345, 2519
EcoRII CCWGG 3 cut(s) 1157, 1266, 2673
EcoRV GATATC 1 cut(s) 2794
EcoT14I CCWWGG 2 cut(s) 92, 2091
EcoT22I ATGCAT 1 cut(s) 1552
EcoT38I GRGCYC 1 cut(s) 857
ErhI CCWWGG 2 cut(s) 92, 2091
Esp3I CGTCTC 1 cut(s) 498
FalI AAGNNNNNCTT 2 cut(s) 2220, 2252
FaqI GGGAC 2 cut(s) 407, 665
FauNDI CATATG 1 cut(s) 346
FbaI TGATCA 1 cut(s) 631
FblI GTMKAC 3 cut(s) 258, 2048, 2766
Fnu4HI GCNGC 5 cut(s) 2274, 2364, 2445, 2576, 2649
FriOI GRGCYC 1 cut(s) 857
Fsp4HI GCNGC 5 cut(s) 2274, 2364, 2445, 2576, 2649
FspBI CTAG 9 cut(s) 41, 524, 927, 1952, 2030, 2039, 2439, 2775, 2822
GlaI GCGC 1 cut(s) 1003
GluI GCNGC 5 cut(s) 2274, 2364, 2445, 2576, 2649
GsuI CTGGAG 2 cut(s) 252, 1180
HaeII RGCGCY 1 cut(s) 1005
HaeIII GGCC 8 cut(s) 158, 299, 690, 836, 1043, 1157, 1690, 1829
HapII CCGG 3 cut(s) 621, 1687, 2052
HgaI GACGC 2 cut(s) 480, 1466
HhaI GCGC 1 cut(s) 1004
Hin6I GCGC 1 cut(s) 1002
HinP1I GCGC 1 cut(s) 1002
HindIII AAGCTT 1 cut(s) 1081
HpaII CCGG 3 cut(s) 621, 1687, 2052
HphI GGTGA 2 cut(s) 611, 2045
Hpy166II GTNNAC 7 cut(s) 8, 259, 985, 1767, 2036, 2049, 2767
Hpy8I GTNNAC 7 cut(s) 8, 259, 985, 1767, 2036, 2049, 2767
Hpy99I CGWCG 1 cut(s) 864
HpyAV CCTTC 7 cut(s) 419, 680, 844, 878, 1202, 1854, 2660
HpyCH4III ACNGT 6 cut(s) 124, 766, 982, 1871, 2635, 2719
HpyCH4IV ACGT 4 cut(s) 910, 1894, 2484, 2816
HpyF10VI GCNNNNNNNGC 5 cut(s) 379, 1457, 2165, 2282, 2847
HpySE526I ACGT 4 cut(s) 910, 1894, 2484, 2816
HspAI GCGC 1 cut(s) 1002
Ksp22I TGATCA 1 cut(s) 631
LguI GCTCTTC 1 cut(s) 2565
LmnI GCTCC 4 cut(s) 233, 345, 533, 2670
Lsp1109I GCAGC 5 cut(s) 2260, 2350, 2431, 2587, 2660
LweI GCATC 8 cut(s) 435, 544, 1469, 1537, 1900, 2125, 2168, 2686
MaeI CTAG 9 cut(s) 41, 524, 927, 1952, 2030, 2039, 2439, 2775, 2822
MaeII ACGT 4 cut(s) 910, 1894, 2484, 2816
MaeIII GTNAC 7 cut(s) 172, 451, 1015, 1454, 1663, 2732, 2817
MflI RGATCY 3 cut(s) 73, 1930, 2857
MhlI GDGCHC 3 cut(s) 857, 1061, 1340
MlsI TGGCCA 1 cut(s) 158
MluNI TGGCCA 1 cut(s) 158
MlyI GAGTC 4 cut(s) 69, 115, 139, 2588
MmeI TCCRAC 4 cut(s) 43, 107, 383, 2040
Mox20I TGGCCA 1 cut(s) 158
Mph1103I ATGCAT 1 cut(s) 1552
MscI TGGCCA 1 cut(s) 158
MseI TTAA 9 cut(s) 285, 459, 990, 1085, 1245, 1641, 2352, 2727, 2867
MslI CAYNNNNRTG 2 cut(s) 1311, 1869
Msp20I TGGCCA 1 cut(s) 158
MspA1I CMGCKG 2 cut(s) 2651, 2850
MspI CCGG 3 cut(s) 621, 1687, 2052
MspR9I CCNGG 3 cut(s) 1159, 1268, 2675
Mva1269I GAATGC 2 cut(s) 778, 2195
MvaI CCWGG 3 cut(s) 1159, 1268, 2675
MwoI GCNNNNNNNGC 5 cut(s) 379, 1457, 2165, 2282, 2847
NcoI CCATGG 2 cut(s) 92, 2091
NdeI CATATG 1 cut(s) 346
NheI GCTAGC 1 cut(s) 1951
NlaIV GGNNCC 6 cut(s) 529, 625, 889, 2672, 2827, 2859
NmuCI GTSAC 4 cut(s) 172, 451, 1454, 2817
NsiI ATGCAT 1 cut(s) 1552
NspI RCATGY 4 cut(s) 1065, 2090, 2495, 2648
PaeI GCATGC 1 cut(s) 2090
PciI ACATGT 2 cut(s) 1061, 2491
PciSI GCTCTTC 1 cut(s) 2565
PctI GAATGC 2 cut(s) 778, 2195
PfeI GAWTC 9 cut(s) 520, 1384, 1889, 2012, 2145, 2369, 2435, 2702, 2783
PfoI TCCNGGA 1 cut(s) 1266
PinAI ACCGGT 1 cut(s) 620
PkrI GCNGC 5 cut(s) 2275, 2365, 2446, 2577, 2650
PleI GAGTC 4 cut(s) 68, 114, 139, 2588
PmaCI CACGTG 1 cut(s) 1895
PmlI CACGTG 1 cut(s) 1895
PpsI GAGTC 4 cut(s) 68, 114, 139, 2588
Ppu21I YACGTR 2 cut(s) 1895, 2485
PscI ACATGT 2 cut(s) 1061, 2491
PshBI ATTAAT 1 cut(s) 2727
Psp6I CCWGG 3 cut(s) 1157, 1266, 2673
PspCI CACGTG 1 cut(s) 1895
PspGI CCWGG 3 cut(s) 1157, 1266, 2673
PspN4I GGNNCC 6 cut(s) 529, 625, 889, 2672, 2827, 2859
PspPI GGNCC 4 cut(s) 298, 834, 1827, 2844
PstI CTGCAG 2 cut(s) 2449, 2476
PstNI CAGNNNCTG 1 cut(s) 1934
PsuI RGATCY 3 cut(s) 73, 1930, 2857
PvuII CAGCTG 2 cut(s) 2651, 2850
RsaI GTAC 9 cut(s) 412, 768, 918, 1234, 1481, 1697, 1766, 2483, 2721
RsaNI GTAC 9 cut(s) 411, 767, 917, 1233, 1480, 1696, 1765, 2482, 2720
RseI CAYNNNNRTG 2 cut(s) 1311, 1869
SapI GCTCTTC 1 cut(s) 2565
SaqAI TTAA 9 cut(s) 285, 459, 990, 1085, 1245, 1641, 2352, 2727, 2867
SatI GCNGC 5 cut(s) 2274, 2364, 2445, 2576, 2649
Sau96I GGNCC 4 cut(s) 298, 834, 1827, 2844
ScaI AGTACT 1 cut(s) 1234
SchI GAGTC 4 cut(s) 69, 115, 139, 2588
ScrFI CCNGG 3 cut(s) 1159, 1268, 2675
SduI GDGCHC 3 cut(s) 857, 1061, 1340
SfaNI GCATC 8 cut(s) 435, 544, 1469, 1537, 1900, 2125, 2168, 2686
SfcI CTRYAG 5 cut(s) 1524, 1926, 2445, 2472, 2634
SinI GGWCC 1 cut(s) 2844
SmiMI CAYNNNNRTG 2 cut(s) 1311, 1869
SmlI CTYRAG 3 cut(s) 797, 2098, 2237
SmoI CTYRAG 3 cut(s) 797, 2098, 2237
SpeI ACTAGT 1 cut(s) 2774
SphI GCATGC 1 cut(s) 2090
SsiI CCGC 1 cut(s) 1515
SspI AATATT 1 cut(s) 420
SspMI CTAG 9 cut(s) 41, 524, 927, 1952, 2030, 2039, 2439, 2775, 2822
StyD4I CCNGG 3 cut(s) 1157, 1266, 2673
StyI CCWWGG 2 cut(s) 92, 2091
TaaI ACNGT 6 cut(s) 124, 766, 982, 1871, 2635, 2719
TaiI ACGT 4 cut(s) 913, 1897, 2487, 2819
TaqI TCGA 6 cut(s) 1285, 1486, 2221, 2250, 2287, 2798
TaqII GACCGA 1 cut(s) 1158
TatI WGTACW 5 cut(s) 410, 1232, 1479, 1695, 2719
TfiI GAWTC 9 cut(s) 520, 1384, 1889, 2012, 2145, 2369, 2435, 2702, 2783
Tru1I TTAA 9 cut(s) 285, 459, 990, 1085, 1245, 1641, 2352, 2727, 2867
Tru9I TTAA 9 cut(s) 285, 459, 990, 1085, 1245, 1641, 2352, 2727, 2867
TscAI CASTG 9 cut(s) 129, 181, 555, 643, 987, 1459, 1876, 2002, 2722
TseFI GTSAC 4 cut(s) 172, 451, 1454, 2817
TseI GCWGC 5 cut(s) 2273, 2363, 2444, 2575, 2648
Tsp45I GTSAC 4 cut(s) 172, 451, 1454, 2817
TspGWI ACGGA 2 cut(s) 774, 2723
TspRI CASTG 9 cut(s) 129, 181, 555, 643, 987, 1459, 1876, 2002, 2722
VpaK11BI GGWCC 1 cut(s) 2844
VspI ATTAAT 1 cut(s) 2727
XagI CCTNNNNNAGG 2 cut(s) 2345, 2519
XapI RAATTY 5 cut(s) 200, 541, 972, 1190, 1248
XbaI TCTAGA 1 cut(s) 2438
XceI RCATGY 4 cut(s) 1065, 2090, 2495, 2648
XcmI CCANNNNNNNNNTGG 1 cut(s) 90
XmiI GTMKAC 3 cut(s) 258, 2048, 2766
XspI CTAG 9 cut(s) 41, 524, 927, 1952, 2030, 2039, 2439, 2775, 2822
ZrmI AGTACT 1 cut(s) 1234
Zsp2I ATGCAT 1 cut(s) 1552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.