pycom09g09930

guanosine tetraphosphate biosynthetic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0011437
Physical Location & Seq
Forward (+)
8052213 .. 8055887
3675 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g09930.2

Sequence Viewer

Length: 2214 bp
ATGACGGTCCCCACAATAGCTCTGTACGCGAGCCCGCCGAGCAGTGTGTGCTCGACGGCGCACCCGTGCCAGATCAACGCGCACCCCTCACACGATTTCGAATTGAGCTCTCGATCTTCGTCGTCGACGGCGTCGCAGAAGCCGGTGGCTGGGGGGCTGTCGTGCCTGTTCTCGTCGCAGACGGTGAAGCACGCGTCATCGTCGTCGTCCAGCTTTTCGGGTGGAGGAGAGGAATTGGGTTCTCGGTGGCACGATAGAGGCGAGGAATTGAGCAGCTCGTTTCGATATTCGGGGAGTAAGTTCAATGGGGCTTCGTTGAACAGGGACCAGAGCCCGGTTTCGGTGTTTCAGGGTCCGGTCTCGTCTTCCAGTAGCGGGGTTTCGGGATCGGGGAGAAGCCCGCCGATGAGAATTGCAAGGGAAAGGTCTAGCAATGGAGATATTAGCTTGAATTCTTTTCGGTGTGGGAGTAATGGGTTGTTTAATGGGTTTGTGAGGGGTGCTTTGGGCTCTTCCTGCATAGATTACGACTCGGCGAGCTTCGAGGCTCAGACTGATGGTTTGGATGTGGGTTCTTCAGCTGTGCTTGATGAATTGACTTTCAACATGGAAGATGGGTTTTTGGAGGGAATTGCTGAGCCTTATGCTAAGGAGTTGCTAGTGGGTGCGCAGCTGCGGCACAAGATTTTCTATGAAGATTTCATTATCAAGGCCTTTTACAAGGCCGAAAAAGCGCATAGAGGGCAGATGCGAGCAAGTGGCGATCCTTATTTGCAGCATTGTGTGGAGACGGCGGTGTTGCTCGCATTGATCGGTGCTAATTCCACAGTTGTTGCTGCAGGGCTTTTGCATGACACGCTTGATGATTCTTTTATGTGTTATGACTACATATTTGGGAAATTTGGAGCTGGGGTTGCTGATTTAGTAGAAGGGGTGTCTAAGCTCAGTCATTTGAGCAAGCTTGCCCGTGATAATGATACAGCATGCAAAACAGTTGAGGCAGATCGCCTGCATACCATGTTCCTTGCCATGGCAGATGCCCGTGCTGTCCTCATTAAATTGGCAGACCGATTGCATAATATGATTACACTAGATGCATTACCCTTGGCTAAACAACAGAGGTTTGCAAAGGAGACTTTGGAGATTTTTGTGCCCTTGGCCAACAGACTTGGAATCTCTAGCTGGAAGGTGCAGCTGGAAAATTTATGTTTTAAGCATCTCAACCCAGATCAGCACAAAGAACTGTCCTCTAAGCTTGTAGATTCGTTTGACGAGGCAATGATTATTTCTGCCACGGAGACATTAGAGCGTGCTCTCAAGGATAAAGCCATTTCCTACCATGTTCTTTGTGGGCGGCATAAGAGCTTGTATAGCATCTACTCTAAAATGTTAAAGAAGAAGCTAAACATGGATGAAATACATGATATTCATGGGCTACGTTTGATTGTCGAAAATGAGCATGATTGTTATGAGGCACTTAAAGTTGTTCACCAGTTGTGGTCCGAGGTCCCAGGAAAGTTCAAGGACTACATAACTCAACCCAAGTGTAATGGGTATCAGTCATTGCACACTGTGGTGATGGGTGAAGGCATGATTCCGCTGGAAGTCCAAATTCGAACAAAGGAGATGCATTTGCAGGCTGAGTTTGGATTTGCAGCTCATTGGAGATACAAGGAAGGTGACTGTAAGCACCCTTCATTTGTGCTTCAAATGGTTGAGTGGGCCAGATGGGTGGTCAGTTGGCAGTGTGAGGCAATGAACAGAGACAGGTCCTCCGTTGGCTGTGACGATTCAATCAAGCCGCCGTGCACGTTTCCTACTCATTCTGATGACTGTCCATATTCTTACAAGCCCCACTGTGGGCAAGACGGGCCGGTGTTTGTCATCATGCTAGAGAATGATAAGATGTCTGTGCAAGAGTTTCCTGCAAACTCCACTGTTATGAATCTGCTGGAAAAAGCTGGGCGAGGGAGCTTGAGATGGACTCCGTACGGGTTCCCACTGAAGGAAGAACTGAGGCCGAGGCTGAACCATGCGCCAGTGAACGATCCAACATGCAAGCTGCAGATGGGGGATGTGGTGGAGCTGACTCCAGCTATTCCCGATAAGTCTTTGACAGAGTACAGAGAAGAGATTCAGCGCATGTACGACCGGGGCACAAGTGTATCGAGTGCTGGGCCTCCTGCTAACCGTATGGCGGGTTGGAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008728 GO:0008893 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009150 GO:0009152 GO:0009163 GO:0009165 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0009991 GO:0010033 GO:0010150 GO:0015969 GO:0015970 GO:0015979 GO:0016020 GO:0016740 GO:0016772 GO:0016778 GO:0016787 GO:0016788 GO:0016794 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0030312 GO:0031667 GO:0032501 GO:0032502 GO:0033865 GO:0033866 GO:0033875 GO:0033993 GO:0034030 GO:0034032 GO:0034033 GO:0034035 GO:0034036 GO:0034404 GO:0034641 GO:0034654 GO:0042221 GO:0042278 GO:0042451 GO:0042455 GO:0042578 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046128 GO:0046129 GO:0046390 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0090693 GO:0097305 GO:0099402 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00230 map00230
Pfam Domains
Protein Families
HD_4 RelA_SpoT

Protein Analysis

738

Amino Acids

81.49

Weight (kDa)

6.2

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14050
fragaria_vesca FvH4_6g34880 FvH4_6g34880 FvH4_6g34880
malus_domestica MD09G1181600.v1.1 MD17G1168000.v1.1
prunus_persica Prupe.3G039700_v2.0.a1
pyrus_communis pycom09g09930 pycom17g15960
rosa_chinensis RchiOBHm_Chr2g0145751
rosa_laevigata RLG00000010129 RLG00000020166
rosa_multiflora Rmu_sc0000473.1_g000007
rosa_roxburghii Rroxscaffold_152G00434730 Rroxscaffold_2G00100470
rosa_rugosa Rorug02G0393100
rosa_samantha Rh2AG447400 Rh2BG459700 Rh2CG434200
rosa_wichuraiana Rw2G036560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 669
AccI GTMKAC 1 cut(s) 125
AccII CGCG 3 cut(s) 29, 80, 194
AciI CCGC 9 cut(s) 35, 375, 401, 676, 794, 1354, 1598, 1802, 2198
AclWI GGATC 3 cut(s) 394, 758, 2042
AcoI YGGCCR 1 cut(s) 1158
AcsI RAATTY 4 cut(s) 451, 899, 1201, 1611
AcuI CTGAAG 2 cut(s) 561, 2024
AcyI GRCGYC 1 cut(s) 131
AdeI CACNNNGTG 1 cut(s) 1573
AfaI GTAC 4 cut(s) 26, 1991, 2123, 2147
AfiI CCNNNNNNNGG 9 cut(s) 149, 334, 340, 375, 1030, 1859, 1860, 2005, 2197
AflIII ACRYGT 1 cut(s) 192
AgsI TTSAA 7 cut(s) 304, 319, 451, 604, 1522, 1709, 1794
AjnI CCWGG 1 cut(s) 1510
AleI CACNNNNGTG 1 cut(s) 1574
Alw21I GWGCWC 4 cut(s) 53, 110, 1315, 1811
Alw26I GTCTC 5 cut(s) 364, 782, 1127, 1292, 1758
Alw44I GTGCAC 1 cut(s) 1807
AlwI GGATC 3 cut(s) 394, 758, 2042
AoxI GGCC 7 cut(s) 711, 723, 1158, 1722, 1871, 2018, 2177
ApaLI GTGCAC 1 cut(s) 1807
ApeKI GCWGC 8 cut(s) 273, 670, 673, 775, 836, 1192, 1655, 2062
ApoI RAATTY 4 cut(s) 451, 899, 1201, 1611
ArsI GACNNNNNNTTYG 4 cut(s) 544, 576, 875, 907
Asp700I GAANNNNTTC 1 cut(s) 2133
AspLEI GCGC 6 cut(s) 61, 82, 670, 736, 2038, 2142
AspS9I GGNCC 9 cut(s) 7, 325, 353, 1500, 1507, 1722, 1770, 1871, 2177
AsuC2I CCSGG 2 cut(s) 335, 2153
AsuHPI GGTGA 5 cut(s) 196, 1481, 1588, 1595, 1691
AsuII TTCGAA 2 cut(s) 99, 1615
AvaII GGWCC 6 cut(s) 7, 325, 353, 1500, 1507, 1770
BaeGI GKGCMC 3 cut(s) 1155, 1811, 2159
BaeI ACNNNNGTAYC 2 cut(s) 2148, 2181
BalI TGGCCA 1 cut(s) 1160
BanII GRGCYC 4 cut(s) 35, 110, 335, 512
BarI GAAGNNNNNNTAC 2 cut(s) 1678, 1710
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 4 cut(s) 53, 110, 1315, 1811
BbvI GCAGC 8 cut(s) 285, 660, 682, 787, 823, 1204, 1667, 2049
BccI CCATC 6 cut(s) 551, 608, 1573, 1722, 1974, 2062
BceAI ACGGC 4 cut(s) 72, 144, 807, 1789
BciT130I CCWGG 1 cut(s) 1512
BcnI CCSGG 2 cut(s) 335, 2153
BcoDI GTCTC 5 cut(s) 364, 782, 1127, 1292, 1758
BfaI CTAG 5 cut(s) 429, 659, 1091, 1179, 1892
BfmI CTRYAG 2 cut(s) 837, 2063
BlpI GCTNAGC 1 cut(s) 636
Bme1390I CCNGG 3 cut(s) 335, 1512, 2153
Bme18I GGWCC 6 cut(s) 7, 325, 353, 1500, 1507, 1770
BmgT120I GGNCC 9 cut(s) 7, 325, 353, 1500, 1507, 1722, 1770, 1871, 2177
BmiI GGNNCC 5 cut(s) 9, 326, 354, 1509, 1997
BmrFI CCNGG 3 cut(s) 335, 1512, 2153
BmsI GCATC 6 cut(s) 738, 1027, 1084, 1225, 1383, 1617
BpiI GAAGAC 1 cut(s) 357
BplI GAGNNNNNCTC 2 cut(s) 1969, 2001
BpmI CTGGAG 1 cut(s) 2076
Bpu10I CCTNAGC 1 cut(s) 648
Bpu1102I GCTNAGC 1 cut(s) 636
Bpu14I TTCGAA 2 cut(s) 99, 1615
BpuEI CTTGAG 2 cut(s) 1301, 1996
BpuMI CCSGG 2 cut(s) 335, 2153
BsaHI GRCGYC 1 cut(s) 131
BsaI GGTCTC 1 cut(s) 364
BsaJI CCNNGG 8 cut(s) 1029, 1104, 1155, 1293, 1503, 1510, 2021, 2152
BsaWI WCCGGW 1 cut(s) 355
BsaXI ACNNNNNCTCC 4 cut(s) 897, 927, 1757, 1787
Bsc4I CCNNNNNNNGG 9 cut(s) 149, 334, 340, 375, 1030, 1859, 1860, 2005, 2197
Bse118I RCCGGY 2 cut(s) 142, 1873
Bse1I ACTGG 3 cut(s) 369, 1492, 2039
Bse3DI GCAATG 4 cut(s) 439, 1284, 1562, 1761
BseBI CCWGG 1 cut(s) 1512
BseDI CCNNGG 8 cut(s) 1029, 1104, 1155, 1293, 1503, 1510, 2021, 2152
BseGI GGATG 3 cut(s) 571, 1417, 2080
BseLI CCNNNNNNNGG 9 cut(s) 149, 334, 340, 375, 1030, 1859, 1860, 2005, 2197
BseMI GCAATG 4 cut(s) 439, 1284, 1562, 1761
BseMII CTCAG 5 cut(s) 563, 627, 958, 1632, 2006
BseNI ACTGG 3 cut(s) 369, 1492, 2039
BseRI GAGGAG 1 cut(s) 240
BseSI GKGCMC 3 cut(s) 1155, 1811, 2159
BseXI GCAGC 8 cut(s) 285, 660, 682, 787, 823, 1204, 1667, 2049
BseYI CCCAGC 4 cut(s) 149, 908, 1961, 2174
BsgI GTGCAG 1 cut(s) 1211
Bsh1236I CGCG 3 cut(s) 29, 80, 194
Bsh1285I CGRYCG 1 cut(s) 2152
BshFI GGCC 7 cut(s) 713, 725, 1160, 1724, 1873, 2020, 2179
BsiEI CGRYCG 1 cut(s) 2152
BsiHKAI GWGCWC 4 cut(s) 53, 110, 1315, 1811
BsiSI CCGG 5 cut(s) 143, 335, 356, 1874, 2152
BsiWI CGTACG 1 cut(s) 1989
BslFI GGGAC 2 cut(s) 338, 1493
BslI CCNNNNNNNGG 9 cut(s) 149, 334, 340, 375, 1030, 1859, 1860, 2005, 2197
BsmAI GTCTC 5 cut(s) 364, 782, 1127, 1292, 1758
BsmBI CGTCTC 1 cut(s) 782
BsmFI GGGAC 2 cut(s) 338, 1493
BsnI GGCC 7 cut(s) 713, 725, 1160, 1724, 1873, 2020, 2179
Bso31I GGTCTC 1 cut(s) 364
Bsp119I TTCGAA 2 cut(s) 99, 1615
Bsp1286I GDGCHC 9 cut(s) 35, 53, 110, 335, 512, 1155, 1315, 1811, 2159
Bsp143I GATC 8 cut(s) 72, 113, 386, 763, 810, 1003, 1228, 2047
Bsp1720I GCTNAGC 1 cut(s) 636
Bsp19I CCATGG 1 cut(s) 1029
BspACI CCGC 9 cut(s) 35, 375, 401, 676, 794, 1354, 1598, 1802, 2198
BspANI GGCC 7 cut(s) 713, 725, 1160, 1724, 1873, 2020, 2179
BspCNI CTCAG 5 cut(s) 562, 628, 957, 1633, 2007
BspFNI CGCG 3 cut(s) 29, 80, 194
BspLI GGNNCC 5 cut(s) 9, 326, 354, 1509, 1997
BspMAI CTGCAG 2 cut(s) 841, 2067
BspPI GGATC 3 cut(s) 394, 758, 2042
BspQI GCTCTTC 1 cut(s) 517
BspT104I TTCGAA 2 cut(s) 99, 1615
BspTNI GGTCTC 1 cut(s) 364
BsrDI GCAATG 4 cut(s) 439, 1284, 1562, 1761
BsrFI RCCGGY 2 cut(s) 142, 1873
BsrI ACTGG 3 cut(s) 369, 1492, 2039
BssAI RCCGGY 2 cut(s) 142, 1873
BssECI CCNNGG 8 cut(s) 1029, 1104, 1155, 1293, 1503, 1510, 2021, 2152
BssMI GATC 8 cut(s) 72, 113, 386, 763, 810, 1003, 1228, 2047
BssNI GRCGYC 1 cut(s) 131
BssT1I CCWWGG 3 cut(s) 1029, 1104, 1155
Bst2UI CCWGG 1 cut(s) 1512
Bst6I CTCTTC 2 cut(s) 517, 2124
BstACI GRCGYC 1 cut(s) 131
BstAPI GCANNNNNTGC 1 cut(s) 48
BstBI TTCGAA 2 cut(s) 99, 1615
BstDEI CTNAG 8 cut(s) 549, 636, 648, 939, 944, 1251, 1641, 2015
BstDSI CCRYGG 2 cut(s) 1029, 1293
BstF5I GGATG 3 cut(s) 571, 1417, 2080
BstFNI CGCG 3 cut(s) 29, 80, 194
BstHHI GCGC 6 cut(s) 61, 82, 670, 736, 2038, 2142
BstKTI GATC 8 cut(s) 75, 116, 389, 766, 813, 1006, 1231, 2050
BstMAI GTCTC 5 cut(s) 364, 782, 1127, 1292, 1758
BstMBI GATC 8 cut(s) 72, 113, 386, 763, 810, 1003, 1228, 2047
BstMCI CGRYCG 1 cut(s) 2152
BstNI CCWGG 1 cut(s) 1512
BstNSI RCATGY 3 cut(s) 987, 2058, 2146
BstSCI CCNGG 3 cut(s) 333, 1510, 2151
BstSFI CTRYAG 2 cut(s) 837, 2063
BstSLI GKGCMC 3 cut(s) 1155, 1811, 2159
BstUI CGCG 3 cut(s) 29, 80, 194
BstV1I GCAGC 8 cut(s) 285, 660, 682, 787, 823, 1204, 1667, 2049
BstV2I GAAGAC 1 cut(s) 357
BstXI CCANNNNNNTGG 1 cut(s) 1732
BsuRI GGCC 7 cut(s) 713, 725, 1160, 1724, 1873, 2020, 2179
BtgI CCRYGG 2 cut(s) 1029, 1293
BtsCI GGATG 3 cut(s) 571, 1417, 2080
BtsI GCAGTG 2 cut(s) 49, 1751
BtsIMutI CAGTG 7 cut(s) 49, 1569, 1751, 1855, 1935, 2000, 2046
CfoI GCGC 6 cut(s) 61, 82, 670, 736, 2038, 2142
Cfr10I RCCGGY 2 cut(s) 142, 1873
Cfr13I GGNCC 9 cut(s) 7, 325, 353, 1500, 1507, 1722, 1770, 1871, 2177
CseI GACGC 2 cut(s) 120, 183
Csp6I GTAC 4 cut(s) 25, 1990, 2122, 2146
CspCI CAANNNNNGTGG 2 cut(s) 814, 849
CviQI GTAC 4 cut(s) 25, 1990, 2122, 2146
DdeI CTNAG 8 cut(s) 549, 636, 648, 939, 944, 1251, 1641, 2015
DpnI GATC 8 cut(s) 74, 115, 388, 765, 812, 1005, 1230, 2049
DpnII GATC 8 cut(s) 72, 113, 386, 763, 810, 1003, 1228, 2047
DraIII CACNNNGTG 1 cut(s) 1573
EaeI YGGCCR 1 cut(s) 1158
Eam1104I CTCTTC 2 cut(s) 517, 2124
EarI CTCTTC 2 cut(s) 517, 2124
Ecl136II GAGCTC 1 cut(s) 108
Eco130I CCWWGG 3 cut(s) 1029, 1104, 1155
Eco147I AGGCCT 1 cut(s) 713
Eco24I GRGCYC 4 cut(s) 35, 110, 335, 512
Eco31I GGTCTC 1 cut(s) 364
Eco47I GGWCC 6 cut(s) 7, 325, 353, 1500, 1507, 1770
Eco53kI GAGCTC 1 cut(s) 108
Eco57I CTGAAG 2 cut(s) 561, 2024
EcoICRI GAGCTC 1 cut(s) 108
EcoO109I RGGNCCY 2 cut(s) 1507, 1770
EcoRI GAATTC 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 1510
EcoT14I CCWWGG 3 cut(s) 1029, 1104, 1155
EcoT22I ATGCAT 2 cut(s) 1099, 1632
EcoT38I GRGCYC 4 cut(s) 35, 110, 335, 512
ErhI CCWWGG 3 cut(s) 1029, 1104, 1155
Esp3I CGTCTC 1 cut(s) 782
FaqI GGGAC 2 cut(s) 338, 1493
FauI CCCGC 4 cut(s) 42, 368, 408, 2191
FblI GTMKAC 1 cut(s) 125
FokI GGATG 3 cut(s) 578, 1424, 2087
FriOI GRGCYC 4 cut(s) 35, 110, 335, 512
FspBI CTAG 5 cut(s) 429, 659, 1091, 1179, 1892
FspI TGCGCA 1 cut(s) 669
GlaI GCGC 6 cut(s) 60, 81, 669, 735, 2037, 2141
GsaI CCCAGC 4 cut(s) 153, 912, 1965, 2178
GsuI CTGGAG 1 cut(s) 2076
HaeIII GGCC 7 cut(s) 713, 725, 1160, 1724, 1873, 2020, 2179
HapII CCGG 5 cut(s) 143, 335, 356, 1874, 2152
HgaI GACGC 2 cut(s) 120, 183
HhaI GCGC 6 cut(s) 61, 82, 670, 736, 2038, 2142
Hin1I GRCGYC 1 cut(s) 131
Hin6I GCGC 6 cut(s) 59, 80, 668, 734, 2036, 2140
HinP1I GCGC 6 cut(s) 59, 80, 668, 734, 2036, 2140
HincII GTYRAC 1 cut(s) 126
HindII GTYRAC 1 cut(s) 126
HindIII AAGCTT 2 cut(s) 959, 1253
HpaII CCGG 5 cut(s) 143, 335, 356, 1874, 2152
HphI GGTGA 5 cut(s) 196, 1481, 1588, 1595, 1691
Hpy166II GTNNAC 4 cut(s) 126, 1489, 1809, 2044
Hpy188I TCNGA 3 cut(s) 552, 1504, 1828
Hpy188III TCNNGA 3 cut(s) 111, 384, 2102
Hpy8I GTNNAC 4 cut(s) 126, 1489, 1809, 2044
Hpy99I CGWCG 8 cut(s) 58, 124, 127, 130, 136, 178, 205, 208
HpyAV CCTTC 6 cut(s) 923, 1180, 1580, 1670, 1704, 1999
HpyCH4IV ACGT 2 cut(s) 1438, 1811
HpyF3I CTNAG 8 cut(s) 549, 636, 648, 939, 944, 1251, 1641, 2015
HpySE526I ACGT 2 cut(s) 1438, 1811
Hsp92I GRCGYC 1 cut(s) 131
HspAI GCGC 6 cut(s) 59, 80, 668, 734, 2036, 2140
Kzo9I GATC 8 cut(s) 72, 113, 386, 763, 810, 1003, 1228, 2047
LguI GCTCTTC 1 cut(s) 517
LmnI GCTCC 3 cut(s) 905, 1971, 2083
Lsp1109I GCAGC 8 cut(s) 285, 660, 682, 787, 823, 1204, 1667, 2049
LweI GCATC 6 cut(s) 738, 1027, 1084, 1225, 1383, 1617
MaeI CTAG 5 cut(s) 429, 659, 1091, 1179, 1892
MaeII ACGT 2 cut(s) 1438, 1811
MaeIII GTNAC 2 cut(s) 1679, 1784
MalI GATC 8 cut(s) 74, 115, 388, 765, 812, 1005, 1230, 2049
MboI GATC 8 cut(s) 72, 113, 386, 763, 810, 1003, 1228, 2047
MboII GAAGA 9 cut(s) 108, 357, 504, 567, 623, 707, 1408, 2021, 2141
MhlI GDGCHC 9 cut(s) 35, 53, 110, 335, 512, 1155, 1315, 1811, 2159
MlsI TGGCCA 1 cut(s) 1160
MluI ACGCGT 1 cut(s) 192
MluNI TGGCCA 1 cut(s) 1160
MlyI GAGTC 3 cut(s) 524, 1978, 2083
MmeI TCCRAC 2 cut(s) 2075, 2183
Mox20I TGGCCA 1 cut(s) 1160
Mph1103I ATGCAT 2 cut(s) 1099, 1632
MroXI GAANNNNTTC 1 cut(s) 2133
MscI TGGCCA 1 cut(s) 1160
MseI TTAA 5 cut(s) 483, 1056, 1212, 1391, 1479
MslI CAYNNNNRTG 3 cut(s) 1574, 1827, 1940
Msp20I TGGCCA 1 cut(s) 1160
MspA1I CMGCKG 4 cut(s) 581, 673, 1195, 1600
MspI CCGG 5 cut(s) 143, 335, 356, 1874, 2152
MspR9I CCNGG 3 cut(s) 335, 1512, 2153
MvaI CCWGG 1 cut(s) 1512
MvnI CGCG 3 cut(s) 29, 80, 194
NciI CCSGG 2 cut(s) 335, 2153
NcoI CCATGG 1 cut(s) 1029
NdeII GATC 8 cut(s) 72, 113, 386, 763, 810, 1003, 1228, 2047
NlaIV GGNNCC 5 cut(s) 9, 326, 354, 1509, 1997
NmeAIII GCCGAG 3 cut(s) 63, 512, 2046
NmuCI GTSAC 2 cut(s) 1679, 1784
NsbI TGCGCA 1 cut(s) 669
NsiI ATGCAT 2 cut(s) 1099, 1632
NspI RCATGY 3 cut(s) 987, 2058, 2146
NspV TTCGAA 2 cut(s) 99, 1615
OliI CACNNNNGTG 1 cut(s) 1574
PaeI GCATGC 1 cut(s) 987
PceI AGGCCT 1 cut(s) 713
PciSI GCTCTTC 1 cut(s) 517
PcsI WCGNNNNNNNCGW 3 cut(s) 62, 128, 258
PdmI GAANNNNTTC 1 cut(s) 2133
PfeI GAWTC 7 cut(s) 866, 1173, 1262, 1594, 1790, 1945, 2134
Pfl23II CGTACG 1 cut(s) 1989
PflFI GACNNNGTC 1 cut(s) 130
PleI GAGTC 3 cut(s) 524, 1978, 2083
PpsI GAGTC 3 cut(s) 524, 1978, 2083
PpuMI RGGWCCY 2 cut(s) 1507, 1770
Psp124BI GAGCTC 1 cut(s) 110
Psp5II RGGWCCY 2 cut(s) 1507, 1770
Psp6I CCWGG 1 cut(s) 1510
PspFI CCCAGC 4 cut(s) 149, 908, 1961, 2174
PspGI CCWGG 1 cut(s) 1510
PspLI CGTACG 1 cut(s) 1989
PspN4I GGNNCC 5 cut(s) 9, 326, 354, 1509, 1997
PspPI GGNCC 9 cut(s) 7, 325, 353, 1500, 1507, 1722, 1770, 1871, 2177
PspPPI RGGWCCY 2 cut(s) 1507, 1770
PstI CTGCAG 2 cut(s) 841, 2067
PsyI GACNNNGTC 1 cut(s) 130
PvuII CAGCTG 3 cut(s) 581, 673, 1195
RsaI GTAC 4 cut(s) 26, 1991, 2123, 2147
RsaNI GTAC 4 cut(s) 25, 1990, 2122, 2146
RseI CAYNNNNRTG 3 cut(s) 1574, 1827, 1940
SacI GAGCTC 1 cut(s) 110
SalI GTCGAC 1 cut(s) 124
SapI GCTCTTC 1 cut(s) 517
SaqAI TTAA 5 cut(s) 483, 1056, 1212, 1391, 1479
Sau3AI GATC 8 cut(s) 72, 113, 386, 763, 810, 1003, 1228, 2047
Sau96I GGNCC 9 cut(s) 7, 325, 353, 1500, 1507, 1722, 1770, 1871, 2177
SchI GAGTC 3 cut(s) 524, 1978, 2083
ScrFI CCNGG 3 cut(s) 335, 1512, 2153
SduI GDGCHC 9 cut(s) 35, 53, 110, 335, 512, 1155, 1315, 1811, 2159
SfaNI GCATC 6 cut(s) 738, 1027, 1084, 1225, 1383, 1617
SfcI CTRYAG 2 cut(s) 837, 2063
SfuI TTCGAA 2 cut(s) 99, 1615
SgrDI CGTCGACG 1 cut(s) 124
SinI GGWCC 6 cut(s) 7, 325, 353, 1500, 1507, 1770
SmiMI CAYNNNNRTG 3 cut(s) 1574, 1827, 1940
SmlI CTYRAG 2 cut(s) 1316, 1975
SmoI CTYRAG 2 cut(s) 1316, 1975
SphI GCATGC 1 cut(s) 987
SseBI AGGCCT 1 cut(s) 713
SsiI CCGC 9 cut(s) 35, 375, 401, 676, 794, 1354, 1598, 1802, 2198
SspMI CTAG 5 cut(s) 429, 659, 1091, 1179, 1892
SstI GAGCTC 1 cut(s) 110
StuI AGGCCT 1 cut(s) 713
StyD4I CCNGG 3 cut(s) 333, 1510, 2151
StyI CCWWGG 3 cut(s) 1029, 1104, 1155
TaiI ACGT 2 cut(s) 1441, 1814
TaqI TCGA 9 cut(s) 53, 99, 112, 125, 283, 543, 1449, 1615, 2168
TaqII GACCGA 1 cut(s) 1083
TatI WGTACW 1 cut(s) 2121
TauI GCSGC 3 cut(s) 679, 1357, 1804
TfiI GAWTC 7 cut(s) 866, 1173, 1262, 1594, 1790, 1945, 2134
Tru1I TTAA 5 cut(s) 483, 1056, 1212, 1391, 1479
Tru9I TTAA 5 cut(s) 483, 1056, 1212, 1391, 1479
TscAI CASTG 7 cut(s) 49, 1576, 1751, 1862, 1942, 2007, 2046
TseFI GTSAC 2 cut(s) 1679, 1784
TseI GCWGC 8 cut(s) 273, 670, 673, 775, 836, 1192, 1655, 2062
Tsp45I GTSAC 2 cut(s) 1679, 1784
TspDTI ATGAA 8 cut(s) 606, 691, 708, 1418, 1428, 1686, 1772, 1958
TspGWI ACGGA 3 cut(s) 1310, 1765, 1977
TspRI CASTG 7 cut(s) 49, 1576, 1751, 1862, 1942, 2007, 2046
Tth111I GACNNNGTC 1 cut(s) 130
VneI GTGCAC 1 cut(s) 1807
VpaK11BI GGWCC 6 cut(s) 7, 325, 353, 1500, 1507, 1770
XapI RAATTY 4 cut(s) 451, 899, 1201, 1611
XceI RCATGY 3 cut(s) 987, 2058, 2146
XcmI CCANNNNNNNNNTGG 1 cut(s) 1346
XmiI GTMKAC 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 2133
XspI CTAG 5 cut(s) 429, 659, 1091, 1179, 1892
Zsp2I ATGCAT 2 cut(s) 1099, 1632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.