RLG00000010129

guanosine tetraphosphate biosynthetic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011437
Physical Location & Seq
Forward (+)
63301018 .. 63304093
3076 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010129

Sequence Viewer

Length: 2103 bp
ATGACGGTCCTGATTGCTCTGTACGCAACGCCGCCGAGCAGTGTGTGCTCGCACGGCACGTTTGATTTTGAATTGAGCTCCAGATCTTCGTCGTCCACGGCTTCGACGCCATCCAAGTCACAAAAGCCGGCGATGGGGGGCTTGTCGTGCTTGTTCTCGTCTCCGACGGTGAAGCATGCTTCTTCTTCTTCTAGCTTTTCTGGTGGAGGAGGTGAGGAATTGGGGTCGATGTGGCCTGATACGGGTGAAGAGTTGAAGGAGTTGAGTAGCTCGTTCCGGTATTTGCAGAGCAAGTTCAGTAGGGCTTCAATGAATAGAGATCAGAGCCCGATTTCGGTGTTTCAGGGTCCGGTTTCGAGTAGCAGTAGTGGGGTTTCGAGCTCTGCTAGGAGTCCTCCGATGAGAATTGCGCGGGAAAGGAGTAGCAATGGAGATGTGAACTTGCGGTGCGGTAGTTATGGGTTGTTTAATGGCTTTGTGAGGGGTGCTTTGGGGTCTTCCTGTGTGGATTATGACTCGCCGAGCTTCGAGGTGCGAAATGATGCTCTAGAGATGAGATCATCAGCTGTGCTGGATGAATTGACTTTCAATATGGAGGATGGGTTTGTGGAGGGAAATTTCGAGCCTTATGCAAGCGAGTTGCTTGTAGGTGCGCAGTTGCAGCACAAGATTTTTTATGAAGAGTTTGTTATCAAGGCTTTCTATGAGGCTGAGAAAGCACACAGAGGGCAGATGCGAGCAAGTGGTGATCCGTATTTGCAGCATTGTGTAGAAACGGCGGTGCTGCTCGCAATGATCGGGGCTAATTCCACAGTTGTTGCTGCAGGGCTTTTGCATGACACGCTTGATGATTCTGTGTCAAAGCTAAGTCATTTGAGCAAGCTTGCCCGTGATAATAATACTGCGTGCAAGACAGTTGAGGCAGACCGTTTGCATACCATGTTTCTTGCCATGGCAGATGCTCGAGCTGTTCTCATTAAATTGGCTGATCGATTGCATAATATGATGACACTGGATGCATTGCCCTTAACTAAACAGCAAAGGTTTGCAAAAGAGACTTTGGAGATCTTTGTGCCATTGGCCCACCGACTAGGAATCTCAAGCTGGAAGGTGCAGCTAGAAAATTTATGTTTTAAGCATCTCAATCCAGACCAACCCAATGACCTATCGTCTAAGCTTGAAGATTCATTTGATGATGCAATGATTACTTCTGCCACAGCGAGATTAGACCAAGCTCTTAGAGAAAAAGCCATTTCCTACCATGTCCTCTGTGGACGGCATAAGAGCTTGTATAGTATCTACTGCAAAATGTTAAAAAAAAAACTGAACATGAGTGAAATTCATGATATCCATGGGCTACGTTTGATTGTTGAGAAAGAGGAAGATTGCTATCAAGCACTGGAAGTTGTTCGCCAGCTATGGACTGAGGTTCCAGGGAAGTTCAAGGACTACATAACACAACGCAAGTGTAATGGGTATCAGTCACTTCACACTGTGGTCATGGGTGAAGGCATGGTTCCCTTAGAAGTGCAAATTCGAACAAAGGAGATGCATCTGCAAGCTGAATTTGGATTTGCTGCTCATTGGAGATATAAGGAAGGTGATTGTAAGCACCCATCGTTTGTGCTTCAGATGGTTGAGTGGGCAAGATGGGTGGTCACTTGGCAGTGCGAGGCAATGAGCAGACCGCCACCTTGCACATTTCCTTCTCATTCCGATGACTGCCCTTATTCTTACAAATCCCACTGTGGTGAAGATGAGCCAGTGTTCGTCATCTTGATAGAGAATGACAAGATGTCTGTGCAAGAATTTCCTGCAAACTCGACAATTATGGATCTGGTTGAAAAAGTTGGGCGCGGGAGCATGAGATGGACAACGTATGGACTCCCATTGAAGGAAGAACTAAGGCCTAGGCTGAACCAAATGCCAGTGAGTGATCCTACATGCAAACTGCAGATGGGGGATGTAGTGGAGCTGACTCCAGCTATTCCTGACAAGTCACTAACAGAGTGCAGGGAAGAGATTCAACGCATGTACGACCGGGGCAGGACTGTATCAAGTGTGGGGTCTCCTGCTAAAAGCATGGTAGGCTGGAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008728 GO:0008893 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009150 GO:0009152 GO:0009163 GO:0009165 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0009991 GO:0010033 GO:0010150 GO:0015969 GO:0015970 GO:0015979 GO:0016020 GO:0016740 GO:0016772 GO:0016778 GO:0016787 GO:0016788 GO:0016794 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0030312 GO:0031667 GO:0032501 GO:0032502 GO:0033865 GO:0033866 GO:0033875 GO:0033993 GO:0034030 GO:0034032 GO:0034033 GO:0034035 GO:0034036 GO:0034404 GO:0034641 GO:0034654 GO:0042221 GO:0042278 GO:0042451 GO:0042455 GO:0042578 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046128 GO:0046129 GO:0046390 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0090693 GO:0097305 GO:0099402 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00230 map00230
Pfam Domains
Protein Families
HD_4 RelA_SpoT

Protein Analysis

701

Amino Acids

78.16

Weight (kDa)

6.19

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD_4 PF13328 233 - 288 5.6e-08 HD domain
HD_4 PF13328 285 - 366 5.5e-19 HD domain
RelA_SpoT PF04607 425 - 534 2.7e-41 Region found in RelA / SpoT proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14050
fragaria_vesca FvH4_6g34880 FvH4_6g34880 FvH4_6g34880
malus_domestica MD09G1181600.v1.1 MD17G1168000.v1.1
prunus_persica Prupe.3G039700_v2.0.a1
pyrus_communis pycom09g09930 pycom17g15960
rosa_chinensis RchiOBHm_Chr2g0145751
rosa_laevigata RLG00000010129 RLG00000020166
rosa_multiflora Rmu_sc0000473.1_g000007
rosa_roxburghii Rroxscaffold_152G00434730 Rroxscaffold_2G00100470
rosa_rugosa Rorug02G0393100
rosa_samantha Rh2AG447400 Rh2BG459700 Rh2CG434200
rosa_wichuraiana Rw2G036560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 654
AccII CGCG 2 cut(s) 412, 1857
AciI CCGC 7 cut(s) 32, 412, 445, 450, 779, 1688, 1857
AclWI GGATC 3 cut(s) 743, 1842, 1931
AcsI RAATTY 6 cut(s) 616, 1123, 1338, 1533, 1565, 1808
AcuI CTGAAG 1 cut(s) 1613
AcyI GRCGYC 1 cut(s) 107
AdeI CACNNNGTG 1 cut(s) 1495
AfaI GTAC 2 cut(s) 23, 2036
AfiI CCNNNNNNNGG 4 cut(s) 134, 242, 334, 1894
AgsI TTSAA 9 cut(s) 71, 256, 309, 589, 1181, 1444, 1844, 1894, 2027
AhdI GACNNNNNGTC 2 cut(s) 1168, 1795
AjnI CCWGG 1 cut(s) 1432
AleI CACNNNNGTG 1 cut(s) 1749
Alw21I GWGCWC 3 cut(s) 50, 80, 383
Alw26I GTCTC 3 cut(s) 165, 1049, 2073
AlwI GGATC 3 cut(s) 743, 1842, 1931
Ama87I CYCGRG 1 cut(s) 963
AoxI GGCC 3 cut(s) 233, 1080, 1907
ApeKI GCWGC 6 cut(s) 661, 760, 784, 821, 1114, 1577
ApoI RAATTY 6 cut(s) 616, 1123, 1338, 1533, 1565, 1808
Asp700I GAANNNNTTC 2 cut(s) 1407, 2022
AspA2I CCTAGG 1 cut(s) 1910
AspLEI GCGC 3 cut(s) 412, 655, 1857
AspS9I GGNCC 3 cut(s) 7, 347, 1081
AsuC2I CCSGG 1 cut(s) 2042
AsuHPI GGTGA 7 cut(s) 181, 224, 257, 758, 1517, 1613, 1763
AsuII TTCGAA 1 cut(s) 1537
AvaI CYCGRG 1 cut(s) 963
AvaII GGWCC 2 cut(s) 7, 347
AvrII CCTAGG 1 cut(s) 1910
BanII GRGCYC 3 cut(s) 80, 329, 383
BbsI GAAGAC 1 cut(s) 489
Bbv12I GWGCWC 3 cut(s) 50, 80, 383
BbvI GCAGC 6 cut(s) 673, 771, 772, 808, 1126, 1564
BccI CCATC 8 cut(s) 118, 127, 593, 1624, 1627, 1644, 1863, 1951
BceAI ACGGC 4 cut(s) 70, 114, 792, 1292
BcgI CGANNNNNNTGC 2 cut(s) 611, 645
BciT130I CCWGG 1 cut(s) 1434
BcnI CCSGG 1 cut(s) 2042
BcoDI GTCTC 3 cut(s) 165, 1049, 2073
BfaI CTAG 6 cut(s) 192, 387, 548, 1091, 1118, 1911
BfmI CTRYAG 2 cut(s) 822, 1952
BglII AGATCT 2 cut(s) 83, 1065
BisI GCNGC 7 cut(s) 32, 662, 761, 785, 822, 1115, 1578
BlnI CCTAGG 1 cut(s) 1910
BlsI GCNGC 7 cut(s) 33, 663, 762, 786, 823, 1116, 1579
Bme1390I CCNGG 2 cut(s) 1434, 2042
Bme18I GGWCC 2 cut(s) 7, 347
BmeRI GACNNNNNGTC 2 cut(s) 1168, 1795
BmeT110I CYCGRG 1 cut(s) 963
BmgT120I GGNCC 3 cut(s) 7, 347, 1081
BmiI GGNNCC 3 cut(s) 348, 1431, 1518
BmrFI CCNGG 2 cut(s) 1434, 2042
BmsI GCATC 8 cut(s) 532, 723, 949, 1006, 1147, 1186, 1539, 1561
BpiI GAAGAC 1 cut(s) 489
BplI GAGNNNNNCTC 2 cut(s) 957, 989
BpmI CTGGAG 2 cut(s) 64, 1965
Bpu14I TTCGAA 1 cut(s) 1537
BpuEI CTTGAG 1 cut(s) 1084
BpuMI CCSGG 1 cut(s) 2042
Bsa29I ATCGAT 1 cut(s) 991
BsaBI GATNNNNATC 1 cut(s) 1389
BsaHI GRCGYC 1 cut(s) 107
BsaI GGTCTC 1 cut(s) 2073
BsaJI CCNNGG 6 cut(s) 96, 951, 1351, 1433, 1910, 2041
BsaWI WCCGGW 2 cut(s) 276, 349
BsaXI ACNNNNNCTCC 2 cut(s) 587, 617
Bsc4I CCNNNNNNNGG 4 cut(s) 134, 242, 334, 1894
Bse118I RCCGGY 1 cut(s) 127
Bse1I ACTGG 4 cut(s) 1017, 1404, 1763, 1928
Bse3DI GCAATG 5 cut(s) 433, 798, 1019, 1206, 1683
Bse8I GATNNNNATC 1 cut(s) 1389
BseBI CCWGG 1 cut(s) 1434
BseCI ATCGAT 1 cut(s) 991
BseDI CCNNGG 6 cut(s) 96, 951, 1351, 1433, 1910, 2041
BseGI GGATG 5 cut(s) 110, 580, 604, 1021, 1969
BseJI GATNNNNATC 1 cut(s) 1389
BseLI CCNNNNNNNGG 4 cut(s) 134, 242, 334, 1894
BseMI GCAATG 5 cut(s) 433, 798, 1019, 1206, 1683
BseMII CTCAG 2 cut(s) 702, 1416
BseNI ACTGG 4 cut(s) 1017, 1404, 1763, 1928
BseRI GAGGAG 1 cut(s) 222
BseXI GCAGC 6 cut(s) 673, 771, 772, 808, 1126, 1564
BsgI GTGCAG 2 cut(s) 1133, 2032
Bsh1236I CGCG 2 cut(s) 412, 1857
Bsh1285I CGRYCG 1 cut(s) 2041
BshFI GGCC 3 cut(s) 235, 1082, 1909
BshVI ATCGAT 1 cut(s) 991
BsiEI CGRYCG 1 cut(s) 2041
BsiHKAI GWGCWC 3 cut(s) 50, 80, 383
BsiHKCI CYCGRG 1 cut(s) 963
BsiSI CCGG 4 cut(s) 128, 277, 350, 2041
BslI CCNNNNNNNGG 4 cut(s) 134, 242, 334, 1894
BsmAI GTCTC 3 cut(s) 165, 1049, 2073
BsmBI CGTCTC 1 cut(s) 165
BsnI GGCC 3 cut(s) 235, 1082, 1909
Bso31I GGTCTC 1 cut(s) 2073
BsoBI CYCGRG 1 cut(s) 963
Bsp119I TTCGAA 1 cut(s) 1537
Bsp1286I GDGCHC 4 cut(s) 50, 80, 329, 383
Bsp143I GATC 9 cut(s) 83, 319, 557, 748, 795, 988, 1065, 1834, 1936
Bsp19I CCATGG 2 cut(s) 951, 1351
BspACI CCGC 7 cut(s) 32, 412, 445, 450, 779, 1688, 1857
BspANI GGCC 3 cut(s) 235, 1082, 1909
BspCNI CTCAG 2 cut(s) 703, 1417
BspDI ATCGAT 1 cut(s) 991
BspFNI CGCG 2 cut(s) 412, 1857
BspHI TCATGA 1 cut(s) 1342
BspLI GGNNCC 3 cut(s) 348, 1431, 1518
BspMAI CTGCAG 2 cut(s) 826, 1956
BspPI GGATC 3 cut(s) 743, 1842, 1931
BspT104I TTCGAA 1 cut(s) 1537
BspTNI GGTCTC 1 cut(s) 2073
BsrDI GCAATG 5 cut(s) 433, 798, 1019, 1206, 1683
BsrFI RCCGGY 1 cut(s) 127
BsrI ACTGG 4 cut(s) 1017, 1404, 1763, 1928
BssAI RCCGGY 1 cut(s) 127
BssECI CCNNGG 6 cut(s) 96, 951, 1351, 1433, 1910, 2041
BssMI GATC 9 cut(s) 83, 319, 557, 748, 795, 988, 1065, 1834, 1936
BssNI GRCGYC 1 cut(s) 107
BssT1I CCWWGG 3 cut(s) 951, 1351, 1910
Bst2UI CCWGG 1 cut(s) 1434
Bst4CI ACNGT 8 cut(s) 7, 169, 814, 916, 929, 1495, 1748, 2053
Bst6I CTCTTC 3 cut(s) 243, 675, 2013
BstACI GRCGYC 1 cut(s) 107
BstAPI GCANNNNNTGC 1 cut(s) 45
BstBI TTCGAA 1 cut(s) 1537
BstDEI CTNAG 7 cut(s) 711, 866, 1173, 1238, 1425, 1522, 1904
BstDSI CCRYGG 3 cut(s) 96, 951, 1351
BstF5I GGATG 5 cut(s) 110, 580, 604, 1021, 1969
BstFNI CGCG 2 cut(s) 412, 1857
BstHHI GCGC 3 cut(s) 412, 655, 1857
BstKTI GATC 9 cut(s) 86, 322, 560, 751, 798, 991, 1068, 1837, 1939
BstMAI GTCTC 3 cut(s) 165, 1049, 2073
BstMBI GATC 9 cut(s) 83, 319, 557, 748, 795, 988, 1065, 1834, 1936
BstMCI CGRYCG 1 cut(s) 2041
BstMWI GCNNNNNNNGC 8 cut(s) 23, 45, 54, 147, 661, 716, 841, 2088
BstNI CCWGG 1 cut(s) 1434
BstNSI RCATGY 3 cut(s) 179, 1947, 2035
BstSCI CCNGG 2 cut(s) 1432, 2040
BstSFI CTRYAG 2 cut(s) 822, 1952
BstUI CGCG 2 cut(s) 412, 1857
BstV1I GCAGC 6 cut(s) 673, 771, 772, 808, 1126, 1564
BstV2I GAAGAC 1 cut(s) 489
BstX2I RGATCY 3 cut(s) 83, 1065, 1834
BstYI RGATCY 3 cut(s) 83, 1065, 1834
Bsu15I ATCGAT 1 cut(s) 991
BsuRI GGCC 3 cut(s) 235, 1082, 1909
BsuTUI ATCGAT 1 cut(s) 991
BtgI CCRYGG 3 cut(s) 96, 951, 1351
BtgZI GCGATG 1 cut(s) 146
BtsCI GGATG 5 cut(s) 110, 580, 604, 1021, 1969
BtsI GCAGTG 2 cut(s) 46, 1673
BtsIMutI CAGTG 8 cut(s) 46, 1010, 1397, 1491, 1673, 1744, 1770, 1935
CciI TCATGA 1 cut(s) 1342
CfoI GCGC 3 cut(s) 412, 655, 1857
Cfr10I RCCGGY 1 cut(s) 127
Cfr13I GGNCC 3 cut(s) 7, 347, 1081
ClaI ATCGAT 1 cut(s) 991
CseI GACGC 1 cut(s) 115
Csp6I GTAC 2 cut(s) 22, 2035
CspCI CAANNNNNGTGG 4 cut(s) 799, 834, 1635, 1670
CviQI GTAC 2 cut(s) 22, 2035
DdeI CTNAG 7 cut(s) 711, 866, 1173, 1238, 1425, 1522, 1904
DpnI GATC 9 cut(s) 85, 321, 559, 750, 797, 990, 1067, 1836, 1938
DpnII GATC 9 cut(s) 83, 319, 557, 748, 795, 988, 1065, 1834, 1936
DraIII CACNNNGTG 1 cut(s) 1495
DriI GACNNNNNGTC 2 cut(s) 1168, 1795
Eam1104I CTCTTC 3 cut(s) 243, 675, 2013
Eam1105I GACNNNNNGTC 2 cut(s) 1168, 1795
EarI CTCTTC 3 cut(s) 243, 675, 2013
Ecl136II GAGCTC 2 cut(s) 78, 381
Eco130I CCWWGG 3 cut(s) 951, 1351, 1910
Eco147I AGGCCT 1 cut(s) 1909
Eco24I GRGCYC 3 cut(s) 80, 329, 383
Eco31I GGTCTC 1 cut(s) 2073
Eco32I GATATC 1 cut(s) 1348
Eco47I GGWCC 2 cut(s) 7, 347
Eco53kI GAGCTC 2 cut(s) 78, 381
Eco57I CTGAAG 1 cut(s) 1613
Eco88I CYCGRG 1 cut(s) 963
EcoICRI GAGCTC 2 cut(s) 78, 381
EcoRII CCWGG 1 cut(s) 1432
EcoRV GATATC 1 cut(s) 1348
EcoT14I CCWWGG 3 cut(s) 951, 1351, 1910
EcoT22I ATGCAT 2 cut(s) 1021, 1554
EcoT38I GRGCYC 3 cut(s) 80, 329, 383
ErhI CCWWGG 3 cut(s) 951, 1351, 1910
Esp3I CGTCTC 1 cut(s) 165
FauI CCCGC 2 cut(s) 405, 1850
Fnu4HI GCNGC 7 cut(s) 32, 662, 761, 785, 822, 1115, 1578
FokI GGATG 5 cut(s) 97, 587, 611, 1028, 1976
FriOI GRGCYC 3 cut(s) 80, 329, 383
Fsp4HI GCNGC 7 cut(s) 32, 662, 761, 785, 822, 1115, 1578
FspBI CTAG 6 cut(s) 192, 387, 548, 1091, 1118, 1911
FspI TGCGCA 1 cut(s) 654
GlaI GCGC 3 cut(s) 411, 654, 1856
GluI GCNGC 7 cut(s) 32, 662, 761, 785, 822, 1115, 1578
GsuI CTGGAG 2 cut(s) 64, 1965
HaeIII GGCC 3 cut(s) 235, 1082, 1909
HapII CCGG 4 cut(s) 128, 277, 350, 2041
HgaI GACGC 1 cut(s) 115
HhaI GCGC 3 cut(s) 412, 655, 1857
Hin1I GRCGYC 1 cut(s) 107
Hin6I GCGC 3 cut(s) 410, 653, 1855
HinP1I GCGC 3 cut(s) 410, 653, 1855
HindIII AAGCTT 2 cut(s) 881, 1175
HinfI GANTC 8 cut(s) 391, 515, 851, 1095, 1184, 1884, 1978, 2023
HpaII CCGG 4 cut(s) 128, 277, 350, 2041
HphI GGTGA 7 cut(s) 181, 224, 257, 758, 1517, 1613, 1763
Hpy166II GTNNAC 3 cut(s) 96, 439, 1274
Hpy188I TCNGA 5 cut(s) 165, 324, 399, 1632, 1717
Hpy188III TCNNGA 7 cut(s) 10, 81, 548, 1148, 1343, 1777, 1991
Hpy8I GTNNAC 3 cut(s) 96, 439, 1274
Hpy99I CGWCG 3 cut(s) 94, 109, 169
HpyAV CCTTC 6 cut(s) 250, 1102, 1502, 1592, 1716, 1888
HpyCH4III ACNGT 8 cut(s) 7, 169, 814, 916, 929, 1495, 1748, 2053
HpyCH4IV ACGT 3 cut(s) 59, 1360, 1877
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 45, 54, 147, 661, 716, 841, 2088
HpyF3I CTNAG 7 cut(s) 711, 866, 1173, 1238, 1425, 1522, 1904
HpySE526I ACGT 3 cut(s) 59, 1360, 1877
Hsp92I GRCGYC 1 cut(s) 107
HspAI GCGC 3 cut(s) 410, 653, 1855
KroI GCCGGC 1 cut(s) 127
KroNI GCCGGC 1 cut(s) 129
Kzo9I GATC 9 cut(s) 83, 319, 557, 748, 795, 988, 1065, 1834, 1936
LmnI GCTCC 3 cut(s) 83, 1860, 1972
Lsp1109I GCAGC 6 cut(s) 673, 771, 772, 808, 1126, 1564
LweI GCATC 8 cut(s) 532, 723, 949, 1006, 1147, 1186, 1539, 1561
MaeI CTAG 6 cut(s) 192, 387, 548, 1091, 1118, 1911
MaeII ACGT 3 cut(s) 59, 1360, 1877
MaeIII GTNAC 4 cut(s) 117, 1482, 1657, 1998
MalI GATC 9 cut(s) 85, 321, 559, 750, 797, 990, 1067, 1836, 1938
MboI GATC 9 cut(s) 83, 319, 557, 748, 795, 988, 1065, 1834, 1936
MflI RGATCY 3 cut(s) 83, 1065, 1834
MhlI GDGCHC 4 cut(s) 50, 80, 329, 383
MlyI GAGTC 4 cut(s) 400, 509, 1878, 1972
MmeI TCCRAC 1 cut(s) 188
Mph1103I ATGCAT 2 cut(s) 1021, 1554
MroNI GCCGGC 1 cut(s) 127
MroXI GAANNNNTTC 2 cut(s) 1407, 2022
MseI TTAA 5 cut(s) 468, 978, 1028, 1134, 1313
MslI CAYNNNNRTG 2 cut(s) 1716, 1749
MspA1I CMGCKG 1 cut(s) 566
MspI CCGG 4 cut(s) 128, 277, 350, 2041
MspR9I CCNGG 2 cut(s) 1434, 2042
MvaI CCWGG 1 cut(s) 1434
MvnI CGCG 2 cut(s) 412, 1857
MwoI GCNNNNNNNGC 8 cut(s) 23, 45, 54, 147, 661, 716, 841, 2088
NaeI GCCGGC 1 cut(s) 129
NciI CCSGG 1 cut(s) 2042
NcoI CCATGG 2 cut(s) 951, 1351
NdeII GATC 9 cut(s) 83, 319, 557, 748, 795, 988, 1065, 1834, 1936
NgoMIV GCCGGC 1 cut(s) 127
NlaIV GGNNCC 3 cut(s) 348, 1431, 1518
NmeAIII GCCGAG 2 cut(s) 60, 546
NmuCI GTSAC 4 cut(s) 117, 1482, 1657, 1998
NsbI TGCGCA 1 cut(s) 654
NsiI ATGCAT 2 cut(s) 1021, 1554
NspI RCATGY 3 cut(s) 179, 1947, 2035
NspV TTCGAA 1 cut(s) 1537
OliI CACNNNNGTG 1 cut(s) 1749
PaeI GCATGC 1 cut(s) 179
PaeR7I CTCGAG 1 cut(s) 963
PagI TCATGA 1 cut(s) 1342
PceI AGGCCT 1 cut(s) 1909
PcsI WCGNNNNNNNCGW 1 cut(s) 56
PdiI GCCGGC 1 cut(s) 129
PdmI GAANNNNTTC 2 cut(s) 1407, 2022
PfeI GAWTC 4 cut(s) 851, 1095, 1184, 2023
PkrI GCNGC 7 cut(s) 33, 663, 762, 786, 823, 1116, 1579
PleI GAGTC 4 cut(s) 399, 509, 1878, 1972
PpsI GAGTC 4 cut(s) 399, 509, 1878, 1972
Psp124BI GAGCTC 2 cut(s) 80, 383
Psp6I CCWGG 1 cut(s) 1432
PspGI CCWGG 1 cut(s) 1432
PspN4I GGNNCC 3 cut(s) 348, 1431, 1518
PspPI GGNCC 3 cut(s) 7, 347, 1081
PspXI VCTCGAGB 1 cut(s) 963
PstI CTGCAG 2 cut(s) 826, 1956
PsuI RGATCY 3 cut(s) 83, 1065, 1834
PvuII CAGCTG 1 cut(s) 566
RsaI GTAC 2 cut(s) 23, 2036
RsaNI GTAC 2 cut(s) 22, 2035
RseI CAYNNNNRTG 2 cut(s) 1716, 1749
SacI GAGCTC 2 cut(s) 80, 383
SaqAI TTAA 5 cut(s) 468, 978, 1028, 1134, 1313
SatI GCNGC 7 cut(s) 32, 662, 761, 785, 822, 1115, 1578
Sau3AI GATC 9 cut(s) 83, 319, 557, 748, 795, 988, 1065, 1834, 1936
Sau96I GGNCC 3 cut(s) 7, 347, 1081
SchI GAGTC 4 cut(s) 400, 509, 1878, 1972
ScrFI CCNGG 2 cut(s) 1434, 2042
SduI GDGCHC 4 cut(s) 50, 80, 329, 383
SfaNI GCATC 8 cut(s) 532, 723, 949, 1006, 1147, 1186, 1539, 1561
SfcI CTRYAG 2 cut(s) 822, 1952
Sfr274I CTCGAG 1 cut(s) 963
SfuI TTCGAA 1 cut(s) 1537
SinI GGWCC 2 cut(s) 7, 347
SlaI CTCGAG 1 cut(s) 963
SmiMI CAYNNNNRTG 2 cut(s) 1716, 1749
SmlI CTYRAG 2 cut(s) 963, 1099
SmoI CTYRAG 2 cut(s) 963, 1099
SphI GCATGC 1 cut(s) 179
SseBI AGGCCT 1 cut(s) 1909
SsiI CCGC 7 cut(s) 32, 412, 445, 450, 779, 1688, 1857
SspMI CTAG 6 cut(s) 192, 387, 548, 1091, 1118, 1911
SstI GAGCTC 2 cut(s) 80, 383
StuI AGGCCT 1 cut(s) 1909
StyD4I CCNGG 2 cut(s) 1432, 2040
StyI CCWWGG 3 cut(s) 951, 1351, 1910
TaaI ACNGT 8 cut(s) 7, 169, 814, 916, 929, 1495, 1748, 2053
TaiI ACGT 3 cut(s) 62, 1363, 1880
TauI GCSGC 1 cut(s) 34
TfiI GAWTC 4 cut(s) 851, 1095, 1184, 2023
Tru1I TTAA 5 cut(s) 468, 978, 1028, 1134, 1313
Tru9I TTAA 5 cut(s) 468, 978, 1028, 1134, 1313
TscAI CASTG 8 cut(s) 46, 1017, 1404, 1498, 1673, 1751, 1770, 1935
TseFI GTSAC 4 cut(s) 117, 1482, 1657, 1998
TseI GCWGC 6 cut(s) 661, 760, 784, 821, 1114, 1577
Tsp45I GTSAC 4 cut(s) 117, 1482, 1657, 1998
TspDTI ATGAA 5 cut(s) 326, 591, 693, 1176, 1331
TspGWI ACGGA 1 cut(s) 741
TspRI CASTG 8 cut(s) 46, 1017, 1404, 1498, 1673, 1751, 1770, 1935
VpaK11BI GGWCC 2 cut(s) 7, 347
XapI RAATTY 6 cut(s) 616, 1123, 1338, 1533, 1565, 1808
XbaI TCTAGA 1 cut(s) 547
XceI RCATGY 3 cut(s) 179, 1947, 2035
XcmI CCANNNNNNNNNTGG 1 cut(s) 1268
XhoI CTCGAG 1 cut(s) 963
XmaJI CCTAGG 1 cut(s) 1910
XmnI GAANNNNTTC 2 cut(s) 1407, 2022
XspI CTAG 6 cut(s) 192, 387, 548, 1091, 1118, 1911
Zsp2I ATGCAT 2 cut(s) 1021, 1554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.