MD17G1168000.v1.1

guanosine tetraphosphate biosynthetic process

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011437
Physical Location & Seq
Forward (+)
16778081 .. 16786159
8079 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1168000.v1.1.491

Sequence Viewer

Length: 2211 bp
ATGACGGTCCCGACAATAGCTCTGTACGCGGCGCACCCGTGCCAGATCAACGCGCACACCTCACACGATTTCGAATTGGGCTCTCGATCTTCTTCGTCGACGGCGTCGACCCCCTCCACGTCGCAGAAGCCGGTGACTGGCGGGCTCTCGTGCCTATTCTCCTCGCCGACGGTGAAGCACGCGTCGTCCTCCTCGAGTTTTTCAGGCGGAGGAGAGGAATTGGGCTCTCTATGGCACGATAGAGGCGAGGAATTGAAGGAATTGAGCAGCTCGTTTCGGTATTCTGGGAGCAAGTTCAATGGGGCTTCGATGAACCGGGACCCGAGCCCGGTTTCAGTGTTTCAGGGTCCGGTTTCGTCTTCCAGTAGCGGTGTTTCAGGGTCGGTGAGGAGCCCACCAATGAGAATTACAAGGGAAAGGTCTGGCAATGGAGATATTAGCTTGAATTCGATTCGGTGTAGAAGTAATGGGTTGTTTAATGGGTTTGTGAGGGGTGCTTTGGGCTCTTCCTGCATAGATTACGACTCGCCGAGCTTCGAGGTTCAGACTGATGGTTTGGATGTGGGTTCATCAGCTGTGCTTGTTGATGATTTGACTTTCAACATGGAAGATGGGTTTTTGGAGGGAATTGCTGAGCCTTATGCTAAGGAGTTGCTTGTGGGTGCGCAGCTGAGGCACAAGATTTTCTATGAAGATTTTATTATCAAGGCCTTTTACGAGGCTGAGAAAGCGCATAGAGGGCAGATGCGAGCAAGTGGCGGTCCTTATTTGCAGCATTGTGTGGAGACGGCGGTGTTGCTCGCATTGATCGGTTCTAATTCGACAATTGTTGCTGCAGGGCTTTTGCATGACACGCTTGATGATTCTTTTATGTGTTATGACTACATATTTGGGAAATTTGGAGCCGGGGTTGCTGATTTAGTAGAAGGGGTGTCTAAGCTCAGTCATTTGAGCAAGCTTGCCCGTGATAATAATACAGCGTGCAAAACAGTTGAGGCAGATCGCCTGCATACCATGTTCCTAGCCATGGCAGATGCCCGTGCTGTCCTCATTAAATTGGCAGACCGATTGCATAATATGATGACACTAGATGCATTACCCTTGGCTAAACAACAGAGGTTTGCAAAGGAGACTTTAGAGATTTTTGTGCCCTTGGCCAACAGACTTGGAATCTCTAGCTGGAAGGTGCAGCTAGAAAATTTATGTTTTAAGCATCTCAACCCGGATCAGCACAATGAACTGTCCTCTAAGCTTGTAGATACGTTTGACGAGGCAATGATTACTTCTGCCACGGAGATATTAGAGCGCGCTCTCAAGGATAAAGCCATTTCCTACCATGTTCTTTGTGGGCGGCATAAGAGCTTGTATAGCATCTACTCCAAAATGTTAAAGAAGAAGCTAAACATGGATGAAATACATGATATTCATGGACTACGTTTGATTGTCGAAAGTGAGGAAGATTGTTACGAGGCACTTAAAGTTGTTCACCAGCTGTGGTTTGAGGTCCCAGGAAAGTTCAAGGACTACATAACTCAACCGAAGTTTAATGGGTATCAGTCGTTGCACACTGTGGTGATGGGTGAAGGCATGATTCCATTGGAAGTTCAAATTCGAACAAAGGAGATGCATTTGCAAGCTGAGTTTGGATTTGCAGCTCATTGGAGATACAAGGAAGGCGACTGTAAGCTCCCCTCGTTTGTGCTTCAGATGGTTGAGTGGGCCAGATGGGTGGTCACTTGGCAGTGTGAGGCAATGAACAGAGACCGCTCCTCCATTGGCTATGACGATTCAATCAAGCCACCCTGCACATTTCCTTCGCATTGTGTTGACTGTCCATATTCTTACAAGCCCCACTGTGGTCAAGACGGGCCGGTGTTTGTCATCATGATTGAGAATGATAAGATGTCTGTGCAAGAGTTTCCTGCAAACTCCACTGTTATGGATCTGCTGGAAAGAGCTGGGCGAGGGAGCTTGAGAAGGACACCATACGGGTTACCACTGAAGGAAGAACTGAGGCCCAGGCTGAATCATGTGCCAGTGAACGATCCTGCATGCGAGCTGCAGATGGGGGATGTGGTGGAACTGACTCCAGCTATTCCGGACAAGTCTTTGACAGAGTACAGAGAAGAGATTCAGCGCATGTACGACCGGGGCTTCAGTGTATCAAGTGCAGGGCCCGCTGCTAATAGTATGGCAGGTTGGAGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008728 GO:0008893 GO:0009058 GO:0009116 GO:0009117 GO:0009119 GO:0009150 GO:0009152 GO:0009163 GO:0009165 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009605 GO:0009611 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0009991 GO:0010033 GO:0010150 GO:0015969 GO:0015970 GO:0015979 GO:0016020 GO:0016740 GO:0016772 GO:0016778 GO:0016787 GO:0016788 GO:0016794 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0030312 GO:0031667 GO:0032501 GO:0032502 GO:0033865 GO:0033866 GO:0033875 GO:0033993 GO:0034030 GO:0034032 GO:0034033 GO:0034035 GO:0034036 GO:0034404 GO:0034641 GO:0034654 GO:0042221 GO:0042278 GO:0042451 GO:0042455 GO:0042578 GO:0042594 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046128 GO:0046129 GO:0046390 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0055086 GO:0071704 GO:0071944 GO:0072521 GO:0072522 GO:0090407 GO:0090693 GO:0097305 GO:0099402 GO:1901068 GO:1901070 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00230 map00230
Pfam Domains
Protein Families
HD_4 RelA_SpoT

Protein Analysis

737

Amino Acids

81.59

Weight (kDa)

5.94

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HD_4 PF13328 237 - 391 5.6e-39 HD domain
HD PF01966 256 - 360 4.7e-08 HD domain
RelA_SpoT PF04607 450 - 559 1.9e-41 Region found in RelA / SpoT proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011437)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14050
fragaria_vesca FvH4_6g34880 FvH4_6g34880 FvH4_6g34880
malus_domestica MD09G1181600.v1.1 MD17G1168000.v1.1
prunus_persica Prupe.3G039700_v2.0.a1
pyrus_communis pycom09g09930 pycom17g15960
rosa_chinensis RchiOBHm_Chr2g0145751
rosa_laevigata RLG00000010129 RLG00000020166
rosa_multiflora Rmu_sc0000473.1_g000007
rosa_roxburghii Rroxscaffold_152G00434730 Rroxscaffold_2G00100470
rosa_rugosa Rorug02G0393100
rosa_samantha Rh2AG447400 Rh2BG459700 Rh2CG434200
rosa_wichuraiana Rw2G036560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 666
Acc36I ACCTGC 1 cut(s) 2186
AccBSI CCGCTC 1 cut(s) 1767
AccI GTMKAC 2 cut(s) 98, 107
AccII CGCG 4 cut(s) 29, 53, 182, 1308
AccIII TCCGGA 1 cut(s) 2098
AciI CCGC 9 cut(s) 29, 141, 207, 369, 759, 791, 1351, 1765, 2178
AclWI GGATC 3 cut(s) 1233, 1950, 2039
AcoI YGGCCR 1 cut(s) 1155
AcsI RAATTY 4 cut(s) 445, 896, 1198, 1608
AcuI CTGAAG 3 cut(s) 1688, 2021, 2140
AcyI GRCGYC 1 cut(s) 104
AdeI CACNNNGTG 1 cut(s) 1570
AfaI GTAC 3 cut(s) 26, 2120, 2144
AfiI CCNNNNNNNGG 4 cut(s) 137, 328, 1027, 1856
AflIII ACRYGT 1 cut(s) 180
AgsI TTSAA 7 cut(s) 256, 298, 445, 601, 1519, 1607, 1791
AjiI CACGTC 1 cut(s) 120
AjnI CCWGG 2 cut(s) 1507, 2018
AleI CACNNNNGTG 1 cut(s) 1571
Alw26I GTCTC 3 cut(s) 779, 1124, 1755
AlwI GGATC 3 cut(s) 1233, 1950, 2039
Ama87I CYCGRG 2 cut(s) 193, 322
Aor13HI TCCGGA 1 cut(s) 2098
AoxI GGCC 6 cut(s) 708, 1155, 1719, 1868, 2015, 2174
ApaI GGGCCC 1 cut(s) 2178
ApeKI GCWGC 8 cut(s) 267, 667, 772, 833, 1189, 1652, 2059, 2180
ApoI RAATTY 4 cut(s) 445, 896, 1198, 1608
ArsI GACNNNNNNTTYG 4 cut(s) 538, 570, 872, 904
Asp700I GAANNNNTTC 1 cut(s) 2130
AspLEI GCGC 7 cut(s) 34, 55, 667, 733, 1308, 1310, 2139
AsuC2I CCSGG 5 cut(s) 317, 329, 907, 1223, 2150
AsuHPI GGTGA 6 cut(s) 145, 184, 397, 1478, 1585, 1592
AsuII TTCGAA 2 cut(s) 72, 1612
AvaI CYCGRG 2 cut(s) 193, 322
AvaII GGWCC 5 cut(s) 7, 319, 347, 761, 1504
BaeGI GKGCMC 2 cut(s) 1152, 2178
BalI TGGCCA 1 cut(s) 1157
BanII GRGCYC 7 cut(s) 83, 147, 227, 329, 395, 506, 2178
BauI CACGAG 1 cut(s) 148
BbsI GAAGAC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 671
BbvI GCAGC 8 cut(s) 279, 679, 784, 820, 1201, 1664, 2046, 2167
BccI CCATC 6 cut(s) 545, 605, 1570, 1702, 1719, 2059
BceAI ACGGC 2 cut(s) 117, 804
BciT130I CCWGG 2 cut(s) 1509, 2020
BcnI CCSGG 5 cut(s) 317, 329, 907, 1223, 2150
BcoDI GTCTC 3 cut(s) 779, 1124, 1755
BfaI CTAG 4 cut(s) 1022, 1088, 1176, 1193
BfmI CTRYAG 2 cut(s) 834, 2060
BfuAI ACCTGC 1 cut(s) 2186
BlpI GCTNAGC 1 cut(s) 633
Bme1390I CCNGG 7 cut(s) 317, 329, 907, 1223, 1509, 2020, 2150
Bme18I GGWCC 5 cut(s) 7, 319, 347, 761, 1504
BmeT110I CYCGRG 2 cut(s) 193, 322
BmgBI CACGTC 1 cut(s) 120
BmiI GGNNCC 8 cut(s) 9, 320, 321, 348, 392, 904, 1506, 2176
BmrFI CCNGG 7 cut(s) 317, 329, 907, 1223, 1509, 2020, 2150
BmsI GCATC 6 cut(s) 735, 1024, 1081, 1222, 1380, 1614
BpiI GAAGAC 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 2073
Bpu10I CCTNAGC 2 cut(s) 645, 671
Bpu1102I GCTNAGC 1 cut(s) 633
Bpu14I TTCGAA 2 cut(s) 72, 1612
BpuEI CTTGAG 2 cut(s) 1298, 1993
BpuMI CCSGG 5 cut(s) 317, 329, 907, 1223, 2150
BsaHI GRCGYC 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 1755
BsaJI CCNNGG 8 cut(s) 906, 1026, 1101, 1152, 1290, 1507, 2018, 2149
BsaWI WCCGGW 2 cut(s) 349, 2098
BsaXI ACNNNNNCTCC 4 cut(s) 894, 924, 1754, 1784
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 328, 1027, 1856
Bse118I RCCGGY 2 cut(s) 130, 1870
Bse1I ACTGG 3 cut(s) 142, 363, 2036
Bse3DI GCAATG 3 cut(s) 433, 1281, 1758
BseAI TCCGGA 1 cut(s) 2098
BseBI CCWGG 2 cut(s) 1509, 2020
BseDI CCNNGG 8 cut(s) 906, 1026, 1101, 1152, 1290, 1507, 2018, 2149
BseGI GGATG 3 cut(s) 565, 1414, 2077
BseLI CCNNNNNNNGG 4 cut(s) 137, 328, 1027, 1856
BseMI GCAATG 3 cut(s) 433, 1281, 1758
BseMII CTCAG 6 cut(s) 624, 662, 714, 955, 1629, 2003
BseNI ACTGG 3 cut(s) 142, 363, 2036
BsePI GCGCGC 1 cut(s) 1306
BseRI GAGGAG 5 cut(s) 151, 181, 225, 403, 1759
BseSI GKGCMC 2 cut(s) 1152, 2178
BseXI GCAGC 8 cut(s) 279, 679, 784, 820, 1201, 1664, 2046, 2167
BseYI CCCAGC 1 cut(s) 1958
BsgI GTGCAG 3 cut(s) 1208, 1789, 2190
Bsh1236I CGCG 4 cut(s) 29, 53, 182, 1308
Bsh1285I CGRYCG 1 cut(s) 2149
BshFI GGCC 6 cut(s) 710, 1157, 1721, 1870, 2017, 2176
BsiEI CGRYCG 1 cut(s) 2149
BsiHKCI CYCGRG 2 cut(s) 193, 322
BsiSI CCGG 9 cut(s) 131, 316, 329, 350, 906, 1223, 1871, 2099, 2149
BslFI GGGAC 2 cut(s) 332, 1490
BslI CCNNNNNNNGG 4 cut(s) 137, 328, 1027, 1856
BsmAI GTCTC 3 cut(s) 779, 1124, 1755
BsmBI CGTCTC 1 cut(s) 779
BsmFI GGGAC 2 cut(s) 332, 1490
BsnI GGCC 6 cut(s) 710, 1157, 1721, 1870, 2017, 2176
Bso31I GGTCTC 1 cut(s) 1755
BsoBI CYCGRG 2 cut(s) 193, 322
Bsp119I TTCGAA 2 cut(s) 72, 1612
Bsp120I GGGCCC 1 cut(s) 2174
Bsp1286I GDGCHC 8 cut(s) 83, 147, 227, 329, 395, 506, 1152, 2178
Bsp13I TCCGGA 1 cut(s) 2098
Bsp143I GATC 7 cut(s) 45, 86, 807, 1000, 1225, 1942, 2044
Bsp1720I GCTNAGC 1 cut(s) 633
Bsp19I CCATGG 1 cut(s) 1026
BspACI CCGC 9 cut(s) 29, 141, 207, 369, 759, 791, 1351, 1765, 2178
BspANI GGCC 6 cut(s) 710, 1157, 1721, 1870, 2017, 2176
BspCNI CTCAG 6 cut(s) 625, 663, 715, 954, 1630, 2004
BspEI TCCGGA 1 cut(s) 2098
BspFNI CGCG 4 cut(s) 29, 53, 182, 1308
BspHI TCATGA 1 cut(s) 1884
BspLI GGNNCC 8 cut(s) 9, 320, 321, 348, 392, 904, 1506, 2176
BspMAI CTGCAG 2 cut(s) 838, 2064
BspMI ACCTGC 1 cut(s) 2186
BspPI GGATC 3 cut(s) 1233, 1950, 2039
BspQI GCTCTTC 1 cut(s) 511
BspT104I TTCGAA 2 cut(s) 72, 1612
BspTNI GGTCTC 1 cut(s) 1755
BsrBI CCGCTC 1 cut(s) 1767
BsrDI GCAATG 3 cut(s) 433, 1281, 1758
BsrFI RCCGGY 2 cut(s) 130, 1870
BsrI ACTGG 3 cut(s) 142, 363, 2036
BssAI RCCGGY 2 cut(s) 130, 1870
BssECI CCNNGG 8 cut(s) 906, 1026, 1101, 1152, 1290, 1507, 2018, 2149
BssHII GCGCGC 1 cut(s) 1306
BssMI GATC 7 cut(s) 45, 86, 807, 1000, 1225, 1942, 2044
BssNI GRCGYC 1 cut(s) 104
BssSI CACGAG 1 cut(s) 148
BssT1I CCWWGG 3 cut(s) 1026, 1101, 1152
Bst2BI CACGAG 1 cut(s) 148
Bst2UI CCWGG 2 cut(s) 1509, 2020
Bst4CI ACNGT 9 cut(s) 7, 172, 991, 1242, 1570, 1682, 1832, 1856, 1936
Bst6I CTCTTC 2 cut(s) 511, 2121
BstACI GRCGYC 1 cut(s) 104
BstBI TTCGAA 2 cut(s) 72, 1612
BstDEI CTNAG 9 cut(s) 633, 645, 671, 723, 936, 941, 1248, 1638, 2012
BstDSI CCRYGG 2 cut(s) 1026, 1290
BstEII GGTNACC 1 cut(s) 1992
BstF5I GGATG 3 cut(s) 565, 1414, 2077
BstFNI CGCG 4 cut(s) 29, 53, 182, 1308
BstHHI GCGC 7 cut(s) 34, 55, 667, 733, 1308, 1310, 2139
BstKTI GATC 7 cut(s) 48, 89, 810, 1003, 1228, 1945, 2047
BstMAI GTCTC 3 cut(s) 779, 1124, 1755
BstMBI GATC 7 cut(s) 45, 86, 807, 1000, 1225, 1942, 2044
BstMCI CGRYCG 1 cut(s) 2149
BstMWI GCNNNNNNNGC 9 cut(s) 26, 510, 673, 728, 739, 853, 911, 1368, 2177
BstNI CCWGG 2 cut(s) 1509, 2020
BstNSI RCATGY 2 cut(s) 2055, 2143
BstPI GGTNACC 1 cut(s) 1992
BstSCI CCNGG 7 cut(s) 315, 327, 905, 1221, 1507, 2018, 2148
BstSFI CTRYAG 2 cut(s) 834, 2060
BstSLI GKGCMC 2 cut(s) 1152, 2178
BstUI CGCG 4 cut(s) 29, 53, 182, 1308
BstV1I GCAGC 8 cut(s) 279, 679, 784, 820, 1201, 1664, 2046, 2167
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 1942
BstXI CCANNNNNNTGG 2 cut(s) 1729, 1939
BstYI RGATCY 1 cut(s) 1942
BsuRI GGCC 6 cut(s) 710, 1157, 1721, 1870, 2017, 2176
BtgI CCRYGG 2 cut(s) 1026, 1290
BtrI CACGTC 1 cut(s) 120
BtsCI GGATG 3 cut(s) 565, 1414, 2077
BtsI GCAGTG 1 cut(s) 1748
BtsIMutI CAGTG 8 cut(s) 342, 1566, 1748, 1852, 1932, 1997, 2043, 2164
BveI ACCTGC 1 cut(s) 2186
CciI TCATGA 1 cut(s) 1884
CfoI GCGC 7 cut(s) 34, 55, 667, 733, 1308, 1310, 2139
Cfr10I RCCGGY 2 cut(s) 130, 1870
CseI GACGC 2 cut(s) 93, 171
Csp6I GTAC 3 cut(s) 25, 2119, 2143
CviQI GTAC 3 cut(s) 25, 2119, 2143
DdeI CTNAG 9 cut(s) 633, 645, 671, 723, 936, 941, 1248, 1638, 2012
DpnI GATC 7 cut(s) 47, 88, 809, 1002, 1227, 1944, 2046
DpnII GATC 7 cut(s) 45, 86, 807, 1000, 1225, 1942, 2044
DraIII CACNNNGTG 1 cut(s) 1570
EaeI YGGCCR 1 cut(s) 1155
Eam1104I CTCTTC 2 cut(s) 511, 2121
EarI CTCTTC 2 cut(s) 511, 2121
EciI GGCGGA 1 cut(s) 222
Eco130I CCWWGG 3 cut(s) 1026, 1101, 1152
Eco147I AGGCCT 1 cut(s) 710
Eco24I GRGCYC 7 cut(s) 83, 147, 227, 329, 395, 506, 2178
Eco31I GGTCTC 1 cut(s) 1755
Eco47I GGWCC 5 cut(s) 7, 319, 347, 761, 1504
Eco57I CTGAAG 3 cut(s) 1688, 2021, 2140
Eco88I CYCGRG 2 cut(s) 193, 322
Eco91I GGTNACC 1 cut(s) 1992
EcoO109I RGGNCCY 3 cut(s) 319, 1504, 2174
EcoO65I GGTNACC 1 cut(s) 1992
EcoRI GAATTC 1 cut(s) 445
EcoRII CCWGG 2 cut(s) 1507, 2018
EcoT14I CCWWGG 3 cut(s) 1026, 1101, 1152
EcoT22I ATGCAT 2 cut(s) 1096, 1629
EcoT38I GRGCYC 7 cut(s) 83, 147, 227, 329, 395, 506, 2178
ErhI CCWWGG 3 cut(s) 1026, 1101, 1152
Esp3I CGTCTC 1 cut(s) 779
FaqI GGGAC 2 cut(s) 332, 1490
FauI CCCGC 2 cut(s) 134, 2185
FblI GTMKAC 2 cut(s) 98, 107
FokI GGATG 3 cut(s) 572, 1421, 2084
FriOI GRGCYC 7 cut(s) 83, 147, 227, 329, 395, 506, 2178
FspBI CTAG 4 cut(s) 1022, 1088, 1176, 1193
FspI TGCGCA 1 cut(s) 666
GlaI GCGC 7 cut(s) 33, 54, 666, 732, 1307, 1309, 2138
GsaI CCCAGC 1 cut(s) 1962
GsuI CTGGAG 1 cut(s) 2073
HaeIII GGCC 6 cut(s) 710, 1157, 1721, 1870, 2017, 2176
HapII CCGG 9 cut(s) 131, 316, 329, 350, 906, 1223, 1871, 2099, 2149
HgaI GACGC 2 cut(s) 93, 171
HhaI GCGC 7 cut(s) 34, 55, 667, 733, 1308, 1310, 2139
Hin1I GRCGYC 1 cut(s) 104
Hin6I GCGC 7 cut(s) 32, 53, 665, 731, 1306, 1308, 2137
HinP1I GCGC 7 cut(s) 32, 53, 665, 731, 1306, 1308, 2137
HincII GTYRAC 3 cut(s) 99, 108, 1828
HindII GTYRAC 3 cut(s) 99, 108, 1828
HindIII AAGCTT 2 cut(s) 956, 1250
HinfI GANTC 9 cut(s) 451, 524, 863, 1170, 1591, 1787, 2026, 2086, 2131
HpaII CCGG 9 cut(s) 131, 316, 329, 350, 906, 1223, 1871, 2099, 2149
HphI GGTGA 6 cut(s) 145, 184, 397, 1478, 1585, 1592
Hpy166II GTNNAC 5 cut(s) 99, 108, 1486, 1828, 2041
Hpy188I TCNGA 2 cut(s) 546, 1707
Hpy188III TCNNGA 5 cut(s) 10, 84, 1862, 1885, 2099
Hpy8I GTNNAC 5 cut(s) 99, 108, 1486, 1828, 2041
Hpy99I CGWCG 6 cut(s) 100, 103, 109, 124, 172, 187
HpyAV CCTTC 8 cut(s) 250, 920, 1177, 1577, 1667, 1824, 1971, 1996
HpyCH4III ACNGT 9 cut(s) 7, 172, 991, 1242, 1570, 1682, 1832, 1856, 1936
HpyCH4IV ACGT 3 cut(s) 119, 1262, 1435
HpyF10VI GCNNNNNNNGC 9 cut(s) 26, 510, 673, 728, 739, 853, 911, 1368, 2177
HpyF3I CTNAG 9 cut(s) 633, 645, 671, 723, 936, 941, 1248, 1638, 2012
HpySE526I ACGT 3 cut(s) 119, 1262, 1435
Hsp92I GRCGYC 1 cut(s) 104
HspAI GCGC 7 cut(s) 32, 53, 665, 731, 1306, 1308, 2137
KflI GGGWCCC 1 cut(s) 319
Kpn2I TCCGGA 1 cut(s) 2098
Kzo9I GATC 7 cut(s) 45, 86, 807, 1000, 1225, 1942, 2044
LguI GCTCTTC 1 cut(s) 511
LmnI GCTCC 6 cut(s) 288, 390, 902, 1692, 1772, 1968
Lsp1109I GCAGC 8 cut(s) 279, 679, 784, 820, 1201, 1664, 2046, 2167
LweI GCATC 6 cut(s) 735, 1024, 1081, 1222, 1380, 1614
MaeI CTAG 4 cut(s) 1022, 1088, 1176, 1193
MaeII ACGT 3 cut(s) 119, 1262, 1435
MaeIII GTNAC 4 cut(s) 133, 1463, 1732, 1992
MalI GATC 7 cut(s) 47, 88, 809, 1002, 1227, 1944, 2046
MbiI CCGCTC 1 cut(s) 1767
MboI GATC 7 cut(s) 45, 86, 807, 1000, 1225, 1942, 2044
MfeI CAATTG 1 cut(s) 825
MflI RGATCY 1 cut(s) 1942
MhlI GDGCHC 8 cut(s) 83, 147, 227, 329, 395, 506, 1152, 2178
MlsI TGGCCA 1 cut(s) 1157
MluI ACGCGT 1 cut(s) 180
MluNI TGGCCA 1 cut(s) 1157
MlyI GAGTC 2 cut(s) 518, 2080
MmeI TCCRAC 1 cut(s) 2180
Mox20I TGGCCA 1 cut(s) 1157
Mph1103I ATGCAT 2 cut(s) 1096, 1629
MroI TCCGGA 1 cut(s) 2098
MroXI GAANNNNTTC 1 cut(s) 2130
MscI TGGCCA 1 cut(s) 1157
MseI TTAA 6 cut(s) 477, 1053, 1209, 1388, 1476, 1545
MslI CAYNNNNRTG 2 cut(s) 1571, 1937
Msp20I TGGCCA 1 cut(s) 1157
MspA1I CMGCKG 4 cut(s) 575, 670, 1492, 2180
MspI CCGG 9 cut(s) 131, 316, 329, 350, 906, 1223, 1871, 2099, 2149
MspR9I CCNGG 7 cut(s) 317, 329, 907, 1223, 1509, 2020, 2150
MunI CAATTG 1 cut(s) 825
MvaI CCWGG 2 cut(s) 1509, 2020
MvnI CGCG 4 cut(s) 29, 53, 182, 1308
MwoI GCNNNNNNNGC 9 cut(s) 26, 510, 673, 728, 739, 853, 911, 1368, 2177
NciI CCSGG 5 cut(s) 317, 329, 907, 1223, 2150
NcoI CCATGG 1 cut(s) 1026
NdeII GATC 7 cut(s) 45, 86, 807, 1000, 1225, 1942, 2044
NlaIV GGNNCC 8 cut(s) 9, 320, 321, 348, 392, 904, 1506, 2176
NmeAIII GCCGAG 1 cut(s) 555
NmuCI GTSAC 2 cut(s) 133, 1732
NsbI TGCGCA 1 cut(s) 666
NsiI ATGCAT 2 cut(s) 1096, 1629
NspI RCATGY 2 cut(s) 2055, 2143
NspV TTCGAA 2 cut(s) 72, 1612
OliI CACNNNNGTG 1 cut(s) 1571
PaeI GCATGC 1 cut(s) 2055
PaeR7I CTCGAG 1 cut(s) 193
PagI TCATGA 1 cut(s) 1884
PauI GCGCGC 1 cut(s) 1306
PceI AGGCCT 1 cut(s) 710
PciSI GCTCTTC 1 cut(s) 511
PcsI WCGNNNNNNNCGW 4 cut(s) 101, 104, 191, 243
PdmI GAANNNNTTC 1 cut(s) 2130
PfeI GAWTC 7 cut(s) 451, 863, 1170, 1591, 1787, 2026, 2131
PflFI GACNNNGTC 1 cut(s) 103
PleI GAGTC 2 cut(s) 518, 2080
PpsI GAGTC 2 cut(s) 518, 2080
PpuMI RGGWCCY 2 cut(s) 319, 1504
Psp5II RGGWCCY 2 cut(s) 319, 1504
Psp6I CCWGG 2 cut(s) 1507, 2018
PspEI GGTNACC 1 cut(s) 1992
PspFI CCCAGC 1 cut(s) 1958
PspGI CCWGG 2 cut(s) 1507, 2018
PspN4I GGNNCC 8 cut(s) 9, 320, 321, 348, 392, 904, 1506, 2176
PspOMI GGGCCC 1 cut(s) 2174
PspPPI RGGWCCY 2 cut(s) 319, 1504
PspXI VCTCGAGB 1 cut(s) 193
PstI CTGCAG 2 cut(s) 838, 2064
PsuI RGATCY 1 cut(s) 1942
PsyI GACNNNGTC 1 cut(s) 103
PteI GCGCGC 1 cut(s) 1306
PvuII CAGCTG 3 cut(s) 575, 670, 1492
RsaI GTAC 3 cut(s) 26, 2120, 2144
RsaNI GTAC 3 cut(s) 25, 2119, 2143
RseI CAYNNNNRTG 2 cut(s) 1571, 1937
SalI GTCGAC 2 cut(s) 97, 106
SapI GCTCTTC 1 cut(s) 511
SaqAI TTAA 6 cut(s) 477, 1053, 1209, 1388, 1476, 1545
Sau3AI GATC 7 cut(s) 45, 86, 807, 1000, 1225, 1942, 2044
SchI GAGTC 2 cut(s) 518, 2080
ScrFI CCNGG 7 cut(s) 317, 329, 907, 1223, 1509, 2020, 2150
SduI GDGCHC 8 cut(s) 83, 147, 227, 329, 395, 506, 1152, 2178
SfaNI GCATC 6 cut(s) 735, 1024, 1081, 1222, 1380, 1614
SfcI CTRYAG 2 cut(s) 834, 2060
Sfr274I CTCGAG 1 cut(s) 193
SfuI TTCGAA 2 cut(s) 72, 1612
SgrDI CGTCGACG 1 cut(s) 97
SinI GGWCC 5 cut(s) 7, 319, 347, 761, 1504
SlaI CTCGAG 1 cut(s) 193
SmiMI CAYNNNNRTG 2 cut(s) 1571, 1937
SmlI CTYRAG 3 cut(s) 193, 1313, 1972
SmoI CTYRAG 3 cut(s) 193, 1313, 1972
SphI GCATGC 1 cut(s) 2055
SseBI AGGCCT 1 cut(s) 710
SsiI CCGC 9 cut(s) 29, 141, 207, 369, 759, 791, 1351, 1765, 2178
SspMI CTAG 4 cut(s) 1022, 1088, 1176, 1193
StuI AGGCCT 1 cut(s) 710
StyD4I CCNGG 7 cut(s) 315, 327, 905, 1221, 1507, 2018, 2148
StyI CCWWGG 3 cut(s) 1026, 1101, 1152
TaaI ACNGT 9 cut(s) 7, 172, 991, 1242, 1570, 1682, 1832, 1856, 1936
TaiI ACGT 3 cut(s) 122, 1265, 1438
TaqII GACCGA 1 cut(s) 1080
TatI WGTACW 1 cut(s) 2118
TauI GCSGC 2 cut(s) 32, 1354
TfiI GAWTC 7 cut(s) 451, 863, 1170, 1591, 1787, 2026, 2131
Tru1I TTAA 6 cut(s) 477, 1053, 1209, 1388, 1476, 1545
Tru9I TTAA 6 cut(s) 477, 1053, 1209, 1388, 1476, 1545
TscAI CASTG 8 cut(s) 342, 1573, 1748, 1859, 1939, 2004, 2043, 2164
TseFI GTSAC 2 cut(s) 133, 1732
TseI GCWGC 8 cut(s) 267, 667, 772, 833, 1189, 1652, 2059, 2180
Tsp45I GTSAC 2 cut(s) 133, 1732
TspDTI ATGAA 7 cut(s) 326, 558, 705, 1251, 1415, 1425, 1769
TspGWI ACGGA 1 cut(s) 1307
TspRI CASTG 8 cut(s) 342, 1573, 1748, 1859, 1939, 2004, 2043, 2164
Tth111I GACNNNGTC 1 cut(s) 103
VpaK11BI GGWCC 5 cut(s) 7, 319, 347, 761, 1504
XapI RAATTY 4 cut(s) 445, 896, 1198, 1608
XceI RCATGY 2 cut(s) 2055, 2143
XcmI CCANNNNNNNNNTGG 1 cut(s) 1343
XhoI CTCGAG 1 cut(s) 193
XmiI GTMKAC 2 cut(s) 98, 107
XmnI GAANNNNTTC 1 cut(s) 2130
XspI CTAG 4 cut(s) 1022, 1088, 1176, 1193
Zsp2I ATGCAT 2 cut(s) 1096, 1629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.