Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001610

Physical Location & Seq

Genomic Coordinates Forward (+)

17014278 .. 17019396

Gene Structure 5119 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1191500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2487 bp

ATGCATCCTCGCCTGAAAAAACTAGGCTTGTCAAAACGACTTAGGGCAGAGTCGTCTGATTTCTGCAATTTGGATTCCACTGAAACACGAACTCCCTCTGCGCGCTCTCTCTCCGTAGCACACTACCTTTTCCCCACCAAAATCCCATCTCAAAGTCTCCGAATTTCGAACCCGAACATGCACGGAGAAGGCGGGGACCGTCGAAAGGAGAGGATGAAAAGCGACTGCAGGAAGATCATGGAGAGCATCAATGCAATGTGTTTCGAAAACCTGAACGACGAGCAGGTGCACTCGCTGGTGCTGATGCGGGAAATCGCGCGCGGAGTTTTGACTAATCGGGTCGAGAAGGTAACCTCCAGCGACGTCAAAAAGGCCTTGTCTTCGAGCATTGAGAAGATGGTGGATTCTTCTAGGGAGGTGGCGAACATTTTGGGTGGGATTTCGTGTGATGGGTTGGGTACGGACGATGTCAAAAAGGTGACGAGTATGAAGGAGATTGCAATGGGGATTGTGAGCAAACGACCCAGAGTTGTAACCATGCTGGGTTGTGAGCCTGGTGAACTGGGTGATGGAGTTGATGATTATGATGTAAGTGAAAAGAATGTGTGGAGTGGAGGGATAGAAAGCGAGTTTAGAAATGAGGATGGGACCCCAAGTGAGTTGGGGGAAAGCCCGGTTTATGGGGTAACACATGCGTCCAATGGGAATTCCCAGTTGGAAGGACTAGAGTCTGAGAGGACGATTGGTGAAGATATGTATGCAGCAGTGGAAGCAGTTTCCAAAGATGAAGACGGTTGGACAGCGATGGGAGGAAGTGTTGCGGACGCAATGATGAATGCTGGCCATGAGGAGCACTTTGGCAGGGTTGAAGGGTTGATGTTCGAAGATGATTGCCTCGTGGTGGTAGCTGATGAGTTTGGGAGTGAAGATTGTCAAGTTGGGGTGCAGAATTCTTTGTCACAGTCAACCCGTCAGCACCCTTTTTTGTGGCCTTCAGATGACGGGATAAACTTAGAGTGGGTTTGCGGCATGATGTCTGCTCTTGAGCAGTCACCAGAGAATTTTCAATCAGTGTTGTCAGTTTCTTTGGTGGATAAATTGATTGATACTGCTTCAAGTATCCTTTGCAAGGAGCCGAACTGTGTGGCAGTTGATTGTCATAAAGAGGATTCAAGAGTTGTTGTAGTTGGCGACATTCATGGACAGTTTCGTGATTTACTCCATCTCTTTGAACATGCTGGGTTTCCCTCTGACAACCAATTTTATGTTTTCAATGGAAATTATGTTAATAGAGGAGCATCGGGCTTAGATGTGTTTATGGTCTTGTTGGCTTGGAAGGTCTTGATGCCTTCTAGAGTATATCTACTTCGTGGGAACCAGGAGTCTAAATATTGCACATCTATTTGGGGTTTTGAGGCAGAGGTTAAGGCTAAAATCGGTGATCAAGGTGAATATGTCTATAATAAATGTCTTGACTGCTTTAAAGAGCTCCCTTTGGCTTCGATCATAGCCAATTGTGTATATACCACCCATGGAGGTCTTTTTCGGAGCATGTGTACTACCCCTTCGAGAAGATCCAAAAGAAAGAGGGAAGAAAAGCTGGAACTAGGTTGTTTAGAAGATTTATCTAAAGTTGAAAGATCTCTTATAGATGCTCCAGATGAAGGACCAAATATATTACTGACTGATGTGCTATGGTCTGATCCATCAAAGGAAGATGGTTTAGTTGAAAAAACATTTCGAGAACGGGGTCTTTGGTGGGGTCCTGATTGTACTGAGGCTTTTCTAAAACAATCCAAGTTAAAGTTGATCATTCGATCACATGAAGGCCCAGATGTAAGGGCGGATCAAGATGAATTTGGTGACATGCTAAGTGGGTACAGTTTGGATCATGCCGGAGACTCGGGGAAACTGTACACACTGTTTAGTGCTGCAAATTATTCACAGTTCGATAAGAAGAGGTATAACAATGAAGGGGTCTATGCTCTTTTGGAGCCTCCTAATTTTCAATCTCCTTCCTTCCACTCATTCATGGCTGCAGGGAGGCCAAAGGTAGATGCTTATGTTGATAATGGTGACAAAGGTTTGGTCGTTGTTGGTGAACCAGACTTGGCATCAGTGTCTGCAACTGGTGCCACCTCTTTGTCTCAAACATCTGTTCCACCTTGTAGTAGTTCTGAGGTTAACTTCGAGGCGTTGGGCATTTCTAATCCCCCTTCTTGCAGTGTTATGGTGGCAGATGATGCAGGTGGCACCCAACTTGTCCAAGTTCCAAAGGCTCCTGTGGTGGAAGGATTGCCTCTGCCTTCTGACTTACAGGAGCCGCACAAAGCTGCTTATAAGTATTTCTTGGAGCTAATTGCTGGTCTGAAGCATATGATTTCAACAAGGGAAGTTCAGACTGGGACGAATGAGTCAACCATGCAAAAGAGAGCTCGGCATCAAATAGAGACGCCACAAGAGGTCAACTTGAAACCTGAGGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009628 GO:0009637 GO:0009785 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0023052 GO:0030522 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0104004 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 map04010

Pfam Domains

Protein Families

Metallophos

Protein Analysis

829

Amino Acids

91.16

Weight (kDa)

4.99

Isoelectric Point (pI)

45.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Metallophos	PF00149	392 - 609	1.7e-25	Calcineurin-like phosphoesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001610)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720 FvH4_6g33720
malus_domestica	MD09G1191500.v1.1
prunus_persica	Prupe.3G047600_v2.0.a1
pyrus_communis	pycom09g10800
rosa_chinensis	RchiOBHm_Chr2g0143611
rosa_laevigata	RLG00000020051 RLG00000033709
rosa_multiflora	Rmu_co8237331.1_g000001 Rmu_sc0002412.1_g000001 Rmu_sc0004340.1_g000029 Rmu_sc0031780.1_g000001
rosa_roxburghii	Rroxscaffold_2G00101600 Rroxscaffold_4G00304410
rosa_rugosa	Rorug02G0384400 Rorug02G0384400 Rorug02G0384400 Rorug02G0384400
rosa_samantha	Rh1BG199600 Rh1DG227400 Rh2AG436200 Rh2BG443800 Rh2CG422400 Rh2DG454200
rosa_wichuraiana	Rw2G035550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2340
AarI	CACCTGC	2 cut(s)	274, 2237
AasI	GACNNNNNNGTC	1 cut(s)	2413
AatII	GACGTC	1 cut(s)	366
Acc36I	ACCTGC	2 cut(s)	274, 2237
AccB1I	GGYRCC	2 cut(s)	2132, 2252
AccII	CGCG	4 cut(s)	103, 317, 319, 321
AciI	CCGC	7 cut(s)	192, 307, 321, 821, 1026, 1844, 2324
AclWI	GGATC	4 cut(s)	1569, 1697, 1854, 1896
AcoI	YGGCCR	1 cut(s)	841
AcsI	RAATTY	5 cut(s)	162, 706, 949, 1060, 1856
AcuI	CTGAAG	2 cut(s)	978, 2390
AcyI	GRCGYC	2 cut(s)	363, 2453
AfaI	GTAC	5 cut(s)	460, 1558, 1774, 1880, 1916
AfiI	CCNNNNNNNGG	5 cut(s)	205, 680, 901, 1129, 1664
AjnI	CCWGG	2 cut(s)	553, 1377
AjuI	GAANNNNNNNTTGG	2 cut(s)	698, 730
AluBI	AGCT	6 cut(s)	908, 1489, 1600, 2333, 2356, 2435
AluI	AGCT	6 cut(s)	908, 1489, 1600, 2333, 2356, 2435
Alw21I	GWGCWC	4 cut(s)	291, 855, 1491, 2437
Alw26I	GTCTC	4 cut(s)	161, 1893, 2151, 2444
Alw44I	GTGCAC	1 cut(s)	287
AlwI	GGATC	4 cut(s)	1569, 1697, 1854, 1896
AlwNI	CAGNNNCTG	1 cut(s)	2123
Ama87I	CYCGRG	1 cut(s)	1903
AoxI	GGCC	5 cut(s)	372, 841, 989, 1828, 2045
ApaLI	GTGCAC	1 cut(s)	287
ApeKI	GCWGC	4 cut(s)	761, 1931, 2036, 2333
ApoI	RAATTY	5 cut(s)	162, 706, 949, 1060, 1856
ArsI	GACNNNNNNTTYG	2 cut(s)	2073, 2105
AspLEI	GCGC	4 cut(s)	103, 105, 319, 321
AspS9I	GGNCC	5 cut(s)	196, 648, 1667, 1763, 1829
AsuC2I	CCSGG	1 cut(s)	674
AsuII	TTCGAA	3 cut(s)	167, 264, 882
AvaI	CYCGRG	1 cut(s)	1903
AvaII	GGWCC	4 cut(s)	196, 648, 1667, 1763
AxyI	CCTNAGG	1 cut(s)	2478
BaeGI	GKGCMC	1 cut(s)	291
BalI	TGGCCA	1 cut(s)	843
BanI	GGYRCC	2 cut(s)	2132, 2252
BanII	GRGCYC	2 cut(s)	1491, 2437
BarI	GAAGNNNNNNTAC	4 cut(s)	1350, 1382, 1549, 1581
BauI	CACGAG	1 cut(s)	896
BbsI	GAAGAC	2 cut(s)	372, 795
Bbv12I	GWGCWC	4 cut(s)	291, 855, 1491, 2437
BbvI	GCAGC	4 cut(s)	773, 1918, 2023, 2320
BccI	CCATC	9 cut(s)	154, 391, 443, 563, 638, 799, 1230, 1712, 1714
BciT130I	CCWGG	2 cut(s)	555, 1379
BciVI	GTATCC	1 cut(s)	1130
BclI	TGATCA	2 cut(s)	1441, 1809
BcnI	CCSGG	1 cut(s)	674
BcoDI	GTCTC	4 cut(s)	161, 1893, 2151, 2444
BfaI	CTAG	5 cut(s)	23, 411, 725, 1353, 1607
BfmI	CTRYAG	2 cut(s)	226, 2037
BfuAI	ACCTGC	2 cut(s)	274, 2237
BfuI	GTATCC	1 cut(s)	1130
BglII	AGATCT	1 cut(s)	1640
BisI	GCNGC	6 cut(s)	762, 1027, 1932, 2037, 2324, 2334
BlsI	GCNGC	6 cut(s)	763, 1028, 1933, 2038, 2325, 2335
Bme1390I	CCNGG	3 cut(s)	555, 674, 1379
Bme18I	GGWCC	4 cut(s)	196, 648, 1667, 1763
BmeT110I	CYCGRG	1 cut(s)	1903
BmgT120I	GGNCC	5 cut(s)	196, 648, 1667, 1763, 1829
BmrFI	CCNGG	3 cut(s)	555, 674, 1379
BmrI	ACTGGG	3 cut(s)	572, 706, 2412
BmuI	ACTGGG	3 cut(s)	572, 706, 2412
BoxI	GACNNNNGTC	1 cut(s)	727
BpiI	GAAGAC	2 cut(s)	372, 795
BpmI	CTGGAG	2 cut(s)	340, 1641
Bpu14I	TTCGAA	3 cut(s)	167, 264, 882
BpuEI	CTTGAG	1 cut(s)	1064
BpuMI	CCSGG	1 cut(s)	674
BsaHI	GRCGYC	2 cut(s)	363, 2453
BsaJI	CCNNGG	1 cut(s)	1531
BsaXI	ACNNNNNCTCC	6 cut(s)	76, 106, 801, 831, 1540, 1570
Bsc4I	CCNNNNNNNGG	5 cut(s)	205, 680, 901, 1129, 1664
Bse1I	ACTGG	4 cut(s)	567, 712, 2134, 2407
Bse21I	CCTNAGG	1 cut(s)	2478
Bse3DI	GCAATG	3 cut(s)	261, 507, 834
BseBI	CCWGG	2 cut(s)	555, 1379
BseDI	CCNNGG	1 cut(s)	1531
BseGI	GGATG	3 cut(s)	4, 219, 649
BseLI	CCNNNNNNNGG	5 cut(s)	205, 680, 901, 1129, 1664
BseMI	GCAATG	3 cut(s)	261, 507, 834
BseMII	CTCAG	4 cut(s)	723, 1767, 2169, 2469
BseNI	ACTGG	4 cut(s)	567, 712, 2134, 2407
BsePI	GCGCGC	2 cut(s)	101, 317
BseRI	GAGGAG	2 cut(s)	863, 1308
BseSI	GKGCMC	1 cut(s)	291
BseXI	GCAGC	4 cut(s)	773, 1918, 2023, 2320
BseYI	CCCAGC	2 cut(s)	541, 1238
BsgI	GTGCAG	1 cut(s)	965
Bsh1236I	CGCG	4 cut(s)	103, 317, 319, 321
BshFI	GGCC	5 cut(s)	374, 843, 991, 1830, 2047
BshNI	GGYRCC	2 cut(s)	2132, 2252
BsiHKAI	GWGCWC	4 cut(s)	291, 855, 1491, 2437
BsiHKCI	CYCGRG	1 cut(s)	1903
BsiSI	CCGG	2 cut(s)	674, 1896
BslFI	GGGAC	3 cut(s)	209, 661, 2419
BslI	CCNNNNNNNGG	5 cut(s)	205, 680, 901, 1129, 1664
BsmAI	GTCTC	4 cut(s)	161, 1893, 2151, 2444
BsmBI	CGTCTC	1 cut(s)	2444
BsmFI	GGGAC	3 cut(s)	209, 661, 2419
BsmI	GAATGC	1 cut(s)	841
BsnI	GGCC	5 cut(s)	374, 843, 991, 1830, 2047
BsoBI	CYCGRG	1 cut(s)	1903
Bsp119I	TTCGAA	3 cut(s)	167, 264, 882
Bsp1286I	GDGCHC	4 cut(s)	291, 855, 1491, 2437
Bsp1407I	TGTACA	1 cut(s)	1914
Bsp19I	CCATGG	1 cut(s)	1531
BspACI	CCGC	7 cut(s)	192, 307, 321, 821, 1026, 1844, 2324
BspANI	GGCC	5 cut(s)	374, 843, 991, 1830, 2047
BspCNI	CTCAG	4 cut(s)	724, 1768, 2170, 2470
BspFNI	CGCG	4 cut(s)	103, 317, 319, 321
BspMAI	CTGCAG	2 cut(s)	230, 2041
BspMI	ACCTGC	2 cut(s)	274, 2237
BspPI	GGATC	4 cut(s)	1569, 1697, 1854, 1896
BspT104I	TTCGAA	3 cut(s)	167, 264, 882
BspT107I	GGYRCC	2 cut(s)	2132, 2252
BsrDI	GCAATG	3 cut(s)	261, 507, 834
BsrGI	TGTACA	1 cut(s)	1914
BsrI	ACTGG	4 cut(s)	567, 712, 2134, 2407
BssECI	CCNNGG	1 cut(s)	1531
BssHII	GCGCGC	2 cut(s)	101, 317
BssNI	GRCGYC	2 cut(s)	363, 2453
BssSI	CACGAG	1 cut(s)	896
BssT1I	CCWWGG	1 cut(s)	1531
Bst2BI	CACGAG	1 cut(s)	896
Bst2UI	CCWGG	2 cut(s)	555, 1379
Bst4CI	ACNGT	9 cut(s)	200, 794, 963, 1142, 1206, 1883, 1914, 1923, 1947
Bst6I	CTCTTC	1 cut(s)	1952
BstACI	GRCGYC	2 cut(s)	363, 2453
BstAPI	GCANNNNNTGC	2 cut(s)	2132, 2243
BstAUI	TGTACA	1 cut(s)	1914
BstBI	TTCGAA	3 cut(s)	167, 264, 882
BstC8I	GCNNGC	3 cut(s)	103, 319, 841
BstDEI	CTNAG	8 cut(s)	41, 732, 1012, 1306, 1776, 1871, 2178, 2478
BstDSI	CCRYGG	1 cut(s)	1531
BstEII	GGTNACC	1 cut(s)	349
BstENI	CCTNNNNNAGG	1 cut(s)	1127
BstF5I	GGATG	3 cut(s)	4, 219, 649
BstFNI	CGCG	4 cut(s)	103, 317, 319, 321
BstHHI	GCGC	4 cut(s)	103, 105, 319, 321
BstMAI	GTCTC	4 cut(s)	161, 1893, 2151, 2444
BstMWI	GCNNNNNNNGC	3 cut(s)	770, 2132, 2243
BstNI	CCWGG	2 cut(s)	555, 1379
BstNSI	RCATGY	5 cut(s)	181, 695, 1238, 1555, 1870
BstPAI	GACNNNNGTC	1 cut(s)	727
BstPI	GGTNACC	1 cut(s)	349
BstSCI	CCNGG	3 cut(s)	553, 672, 1377
BstSFI	CTRYAG	2 cut(s)	226, 2037
BstSLI	GKGCMC	1 cut(s)	291
BstUI	CGCG	4 cut(s)	103, 317, 319, 321
BstV1I	GCAGC	4 cut(s)	773, 1918, 2023, 2320
BstV2I	GAAGAC	2 cut(s)	372, 795
BstX2I	RGATCY	2 cut(s)	1574, 1640
BstYI	RGATCY	2 cut(s)	1574, 1640
Bsu36I	CCTNAGG	1 cut(s)	2478
BsuI	GTATCC	1 cut(s)	1130
BsuRI	GGCC	5 cut(s)	374, 843, 991, 1830, 2047
BtgI	CCRYGG	1 cut(s)	1531
BtgZI	GCGATG	1 cut(s)	818
BtsCI	GGATG	3 cut(s)	4, 219, 649
BtsI	GCAGTG	2 cut(s)	771, 2230
BtsIMutI	CAGTG	6 cut(s)	78, 771, 1077, 1919, 2124, 2230
BveI	ACCTGC	2 cut(s)	274, 2237
Cac8I	GCNNGC	3 cut(s)	103, 319, 841
CaiI	CAGNNNCTG	1 cut(s)	2123
CfoI	GCGC	4 cut(s)	103, 105, 319, 321
Cfr13I	GGNCC	5 cut(s)	196, 648, 1667, 1763, 1829
CseI	GACGC	3 cut(s)	684, 833, 2461
Csp6I	GTAC	5 cut(s)	459, 1557, 1773, 1879, 1915
CviQI	GTAC	5 cut(s)	459, 1557, 1773, 1879, 1915
DdeI	CTNAG	8 cut(s)	41, 732, 1012, 1306, 1776, 1871, 2178, 2478
DraI	TTTAAA	1 cut(s)	1483
DrdI	GACNNNNNNGTC	1 cut(s)	2413
DseDI	GACNNNNNNGTC	1 cut(s)	2413
EaeI	YGGCCR	1 cut(s)	841
Eam1104I	CTCTTC	1 cut(s)	1952
EarI	CTCTTC	1 cut(s)	1952
EciI	GGCGGA	1 cut(s)	1859
Ecl136II	GAGCTC	2 cut(s)	1489, 2435
Eco130I	CCWWGG	1 cut(s)	1531
Eco147I	AGGCCT	1 cut(s)	374
Eco24I	GRGCYC	2 cut(s)	1491, 2437
Eco47I	GGWCC	4 cut(s)	196, 648, 1667, 1763
Eco53kI	GAGCTC	2 cut(s)	1489, 2435
Eco57I	CTGAAG	2 cut(s)	978, 2390
Eco81I	CCTNAGG	1 cut(s)	2478
Eco88I	CYCGRG	1 cut(s)	1903
Eco91I	GGTNACC	1 cut(s)	349
EcoICRI	GAGCTC	2 cut(s)	1489, 2435
EcoNI	CCTNNNNNAGG	1 cut(s)	1127
EcoO109I	RGGNCCY	2 cut(s)	648, 1763
EcoO65I	GGTNACC	1 cut(s)	349
EcoRI	GAATTC	2 cut(s)	706, 949
EcoRII	CCWGG	2 cut(s)	553, 1377
EcoT14I	CCWWGG	1 cut(s)	1531
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	2 cut(s)	1491, 2437
ErhI	CCWWGG	1 cut(s)	1531
Esp3I	CGTCTC	1 cut(s)	2444
FalI	AAGNNNNNCTT	2 cut(s)	2333, 2365
FaqI	GGGAC	3 cut(s)	209, 661, 2419
FauI	CCCGC	2 cut(s)	185, 300
FauNDI	CATATG	1 cut(s)	2376
FbaI	TGATCA	2 cut(s)	1441, 1809
Fnu4HI	GCNGC	6 cut(s)	762, 1027, 1932, 2037, 2324, 2334
FokI	GGATG	2 cut(s)	226, 656
FriOI	GRGCYC	2 cut(s)	1491, 2437
Fsp4HI	GCNGC	6 cut(s)	762, 1027, 1932, 2037, 2324, 2334
FspBI	CTAG	5 cut(s)	23, 411, 725, 1353, 1607
GlaI	GCGC	4 cut(s)	102, 104, 318, 320
GluI	GCNGC	6 cut(s)	762, 1027, 1932, 2037, 2324, 2334
GsaI	CCCAGC	2 cut(s)	545, 1242
GsuI	CTGGAG	2 cut(s)	340, 1641
HaeIII	GGCC	5 cut(s)	374, 843, 991, 1830, 2047
HapII	CCGG	2 cut(s)	674, 1896
HgaI	GACGC	3 cut(s)	684, 833, 2461
HhaI	GCGC	4 cut(s)	103, 105, 319, 321
Hin1I	GRCGYC	2 cut(s)	363, 2453
Hin6I	GCGC	4 cut(s)	101, 103, 317, 319
HinP1I	GCGC	4 cut(s)	101, 103, 317, 319
HincII	GTYRAC	4 cut(s)	966, 2185, 2418, 2467
HindII	GTYRAC	4 cut(s)	966, 2185, 2418, 2467
HinfI	GANTC	8 cut(s)	50, 74, 404, 728, 1169, 1382, 1901, 2414
HpaI	GTTAAC	1 cut(s)	2185
HpaII	CCGG	2 cut(s)	674, 1896
Hpy166II	GTNNAC	9 cut(s)	289, 560, 966, 1557, 1917, 2102, 2185, 2418, 2467
Hpy8I	GTNNAC	9 cut(s)	289, 560, 966, 1557, 1917, 2102, 2185, 2418, 2467
Hpy99I	CGWCG	3 cut(s)	204, 281, 365
HpyCH4III	ACNGT	9 cut(s)	200, 794, 963, 1142, 1206, 1883, 1914, 1923, 1947
HpyCH4IV	ACGT	1 cut(s)	363
HpyF10VI	GCNNNNNNNGC	3 cut(s)	770, 2132, 2243
HpyF3I	CTNAG	8 cut(s)	41, 732, 1012, 1306, 1776, 1871, 2178, 2478
HpySE526I	ACGT	1 cut(s)	363
Hsp92I	GRCGYC	2 cut(s)	363, 2453
HspAI	GCGC	4 cut(s)	101, 103, 317, 319
KflI	GGGWCCC	1 cut(s)	648
Ksp22I	TGATCA	2 cut(s)	1441, 1809
KspAI	GTTAAC	1 cut(s)	2185
Lsp1109I	GCAGC	4 cut(s)	773, 1918, 2023, 2320
MaeI	CTAG	5 cut(s)	23, 411, 725, 1353, 1607
MaeII	ACGT	1 cut(s)	363
MaeIII	GTNAC	8 cut(s)	349, 478, 532, 685, 957, 1050, 1862, 2075
MauBI	CGCGCGCG	1 cut(s)	317
MfeI	CAATTG	1 cut(s)	1513
MflI	RGATCY	2 cut(s)	1574, 1640
MhlI	GDGCHC	4 cut(s)	291, 855, 1491, 2437
MlsI	TGGCCA	1 cut(s)	843
MluNI	TGGCCA	1 cut(s)	843
MlyI	GAGTC	5 cut(s)	59, 737, 1391, 1895, 2423
MmeI	TCCRAC	2 cut(s)	696, 776
Mox20I	TGGCCA	1 cut(s)	843
Mph1103I	ATGCAT	1 cut(s)	6
MscI	TGGCCA	1 cut(s)	843
MseI	TTAA	5 cut(s)	1287, 1425, 1482, 1802, 2184
Msp20I	TGGCCA	1 cut(s)	843
MspI	CCGG	2 cut(s)	674, 1896
MspR9I	CCNGG	3 cut(s)	555, 674, 1379
MunI	CAATTG	1 cut(s)	1513
Mva1269I	GAATGC	1 cut(s)	841
MvaI	CCWGG	2 cut(s)	555, 1379
MvnI	CGCG	4 cut(s)	103, 317, 319, 321
MwoI	GCNNNNNNNGC	3 cut(s)	770, 2132, 2243
NciI	CCSGG	1 cut(s)	674
NcoI	CCATGG	1 cut(s)	1531
NdeI	CATATG	1 cut(s)	2376
NmeAIII	GCCGAG	1 cut(s)	2416
NmuCI	GTSAC	5 cut(s)	478, 957, 1050, 1862, 2075
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	5 cut(s)	181, 695, 1238, 1555, 1870
NspV	TTCGAA	3 cut(s)	167, 264, 882
PaqCI	CACCTGC	2 cut(s)	274, 2237
PauI	GCGCGC	2 cut(s)	101, 317
PceI	AGGCCT	1 cut(s)	374
PctI	GAATGC	1 cut(s)	841
PfeI	GAWTC	3 cut(s)	74, 404, 1169
PflFI	GACNNNGTC	1 cut(s)	467
PkrI	GCNGC	6 cut(s)	763, 1028, 1933, 2038, 2325, 2335
PleI	GAGTC	5 cut(s)	58, 736, 1390, 1895, 2422
PpsI	GAGTC	5 cut(s)	58, 736, 1390, 1895, 2422
PpuMI	RGGWCCY	2 cut(s)	648, 1763
PshAI	GACNNNNGTC	1 cut(s)	727
PsiI	TTATAA	1 cut(s)	2340
Psp124BI	GAGCTC	2 cut(s)	1491, 2437
Psp5II	RGGWCCY	2 cut(s)	648, 1763
Psp6I	CCWGG	2 cut(s)	553, 1377
PspEI	GGTNACC	1 cut(s)	349
PspFI	CCCAGC	2 cut(s)	541, 1238
PspGI	CCWGG	2 cut(s)	553, 1377
PspPI	GGNCC	5 cut(s)	196, 648, 1667, 1763, 1829
PspPPI	RGGWCCY	2 cut(s)	648, 1763
PstI	CTGCAG	2 cut(s)	230, 2041
PstNI	CAGNNNCTG	1 cut(s)	2123
PsuI	RGATCY	2 cut(s)	1574, 1640
PsyI	GACNNNGTC	1 cut(s)	467
PteI	GCGCGC	2 cut(s)	101, 317
RsaI	GTAC	5 cut(s)	460, 1558, 1774, 1880, 1916
RsaNI	GTAC	5 cut(s)	459, 1557, 1773, 1879, 1915
SacI	GAGCTC	2 cut(s)	1491, 2437
SaqAI	TTAA	5 cut(s)	1287, 1425, 1482, 1802, 2184
SatI	GCNGC	6 cut(s)	762, 1027, 1932, 2037, 2324, 2334
Sau96I	GGNCC	5 cut(s)	196, 648, 1667, 1763, 1829
SchI	GAGTC	5 cut(s)	59, 737, 1391, 1895, 2423
ScrFI	CCNGG	3 cut(s)	555, 674, 1379
SduI	GDGCHC	4 cut(s)	291, 855, 1491, 2437
SfcI	CTRYAG	2 cut(s)	226, 2037
SfuI	TTCGAA	3 cut(s)	167, 264, 882
SinI	GGWCC	4 cut(s)	196, 648, 1667, 1763
SmlI	CTYRAG	1 cut(s)	1043
SmoI	CTYRAG	1 cut(s)	1043
SseBI	AGGCCT	1 cut(s)	374
SsiI	CCGC	7 cut(s)	192, 307, 321, 821, 1026, 1844, 2324
SspI	AATATT	1 cut(s)	1391
SspMI	CTAG	5 cut(s)	23, 411, 725, 1353, 1607
SstI	GAGCTC	2 cut(s)	1491, 2437
StuI	AGGCCT	1 cut(s)	374
StyD4I	CCNGG	3 cut(s)	553, 672, 1377
StyI	CCWWGG	1 cut(s)	1531
TaaI	ACNGT	9 cut(s)	200, 794, 963, 1142, 1206, 1883, 1914, 1923, 1947
TaiI	ACGT	1 cut(s)	366
TatI	WGTACW	3 cut(s)	1556, 1772, 1914
TauI	GCSGC	2 cut(s)	1029, 2326
TfiI	GAWTC	3 cut(s)	74, 404, 1169
Tru1I	TTAA	5 cut(s)	1287, 1425, 1482, 1802, 2184
Tru9I	TTAA	5 cut(s)	1287, 1425, 1482, 1802, 2184
TscAI	CASTG	6 cut(s)	85, 771, 1077, 1926, 2124, 2230
TseFI	GTSAC	5 cut(s)	478, 957, 1050, 1862, 2075
TseI	GCWGC	4 cut(s)	761, 1931, 2036, 2333
Tsp45I	GTSAC	5 cut(s)	478, 957, 1050, 1862, 2075
TspGWI	ACGGA	3 cut(s)	103, 198, 476
TspRI	CASTG	6 cut(s)	85, 771, 1077, 1926, 2124, 2230
Tth111I	GACNNNGTC	1 cut(s)	467
VneI	GTGCAC	1 cut(s)	287
VpaK11BI	GGWCC	4 cut(s)	196, 648, 1667, 1763
XagI	CCTNNNNNAGG	1 cut(s)	1127
XapI	RAATTY	5 cut(s)	162, 706, 949, 1060, 1856
XbaI	TCTAGA	1 cut(s)	1352
XceI	RCATGY	5 cut(s)	181, 695, 1238, 1555, 1870
XspI	CTAG	5 cut(s)	23, 411, 725, 1353, 1607
ZraI	GACGTC	1 cut(s)	364
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.